A method for artificially collecting reproductives of a fungus-growing termite
By simulating the principles of termite swarming and microenvironment ecology, and combining changes in temperature, humidity, light, and air pressure, the method utilizes ultraviolet light traps and collection trays to collect adult termites. This solves the problems of narrow time windows and infection risks associated with collecting adult termites under natural conditions, and achieves safe and efficient collection of adult termites and seedling cultivation.
Patent Information
- Application Number
- CN202410649288.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-23
- Publication Date
- 2025-11-28
- Estimated Expiration
- 2044-05-23
AI Technical Summary
Under natural conditions, termite adults swarm in large numbers, but the time window is narrow, making efficient collection difficult. They are also easily preyed upon by natural enemies or infected by pathogenic microorganisms, resulting in a low reproductive success rate. Therefore, they are not suitable for termite insect protein production and seedling cultivation.
By simulating the principles of termite swarming and microenvironment ecology, and using artificial stimuli such as changes in temperature, humidity, light, and air pressure, combined with disinfectant treatment, adult termites are captured using ultraviolet light traps and collection trays, achieving safe and efficient collection.
It enables safe and efficient collection of adult termites with long working cycles, reduces the risk of pathogenic microorganism infection, and improves the reproduction success rate. It is suitable for termite insect protein production and seedling cultivation.
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of termite cultivation, and particularly relates to a method for artificially collecting alates of cultivated termites. BACKGROUND
[0002] Cultivated termites are important ecological engineers in the subtropical and tropical regions of Asia and Africa, and are important soil animals that play a key role in stabilizing the ecological system of the distribution area. The mass alate behavior of the termites provides a rich source of nitrogen nutrients in the form of prey and dead carcasses for the environment, including a rich source of food for other predatory animals, including birds, predatory ants, bats and other ant-eating animals. The nitrogen nutrients produced by the decomposition of dead termite adults provide fertilizer for various shallow-rooted plants and seedlings. However, in recent years, with the changes in the environment and the excessive use of agricultural chemicals, the number of cultivated termite nests has been rapidly decreasing year by year, affecting the stability and safety of the agroforestry ecosystem.
[0003] In order to protect and develop the ecological service function of cultivated termites, the efficient cultivation of cultivated termites has been achieved through research and development, and the production technology of termite seedlings is a key link in achieving efficient cultivation. The alate female and male adults of termites are the future queen and king of the termite nest, and need to survive in the nest for decades to maintain the reproductive and productive capacity of the nest. Under natural conditions, although the alate adults of termites have a large amount of alates, they usually only fly out of the nest as a whole under specific meteorological conditions, which makes the time window for natural utilization narrow, only a few hours, and it is not possible to efficiently and completely collect and utilize them naturally. The alate adults that fly out of the nest are often preyed upon by natural enemies and infected with pathogenic microorganisms, and are not suitable for the production of termite insect protein, let alone the production of termite seedlings. The reproductive success rate of alate adults that fly out of the nest is extremely low, usually around one in ten thousand, and is not suitable for the protection and breeding of cultivated termite nests.
[0004] In view of the above, the present application is proposed. SUMMARY
[0005] The purpose of the present application is to provide a method for artificially collecting alate adults of cultivated termites. The present application realizes the safe, efficient and clean collection of various types of alate adults of cultivated termites through the combination of termite alate behavior and microenvironment ecology.
[0006] In order to achieve the above-mentioned purpose of the present application, the following technical solutions are adopted:
[0007] The present application provides a method for artificially collecting alate adults of cultivated termites, which comprises the following steps:
[0008] (a) Determination of the alate area:
[0009] During the swarming period of the rearing termites, the mud line of termite activity is found around the location of the rearing termite nest growth area, the surface soil is removed, the area of flat round and fan-shaped holes is found, and the swarming area of the rearing termites is determined;
[0010] (b) Humidity adjustment:
[0011] Within 1-3 days of the swarming period of the rearing termites, 0.5-2 t of clean water is used to soak the soil within 2-10 m 2 around the swarming area;
[0012] (c) Temperature and light control:
[0013] When the humidity is adjusted, the swarming area is found to have termite activity and build a swarming hole, a heat preservation shed is built to cover the swarming area, and the ground surface temperature in the heat preservation shed is controlled at 17-29℃;
[0014] (d) Swarming stimulation:
[0015] When the rearing termites start to build swarming holes to prepare for swarming, the rearing termites swarming after rain and after the rain are collected, a light rain is simulated in the heat preservation shed to stimulate the swarming; the pressure-sensitive rearing termites swarming after the rain clears are collected, and a sealed shed is used for sealing and pressurization to stimulate the swarming;
[0016] (e) Lamp collection:
[0017] When the rearing termites swarm at night, a purple light collection tray is set on one side of the swarming area before the adults appear at the swarming hole and start to swarm out of the swarming hole to collect the swarming adults;
[0018] When the rearing termites swarm during the day, a collection net is set on the top of the heat preservation shed to collect the swarming adults by taking advantage of the habit of the adults flying upward toward the light.
[0019] Preferably, in step (d), a disinfectant is also sprayed on the entire swarming area.
[0020] Preferably, the disinfectant is 0.05%-0.5% sodium hypochlorite disinfectant, saturated ozone water disinfectant, and 0.5% hydrogen peroxide disinfectant.
[0021] Preferably, in step (a), the swarming period of the rearing termites is as follows:
[0022] The swarming period of the blackish-winged soil termites, Hainan soil termites, and slender-jawed soil termites in tropical climates is from February to April; the swarming period of the blackish-winged soil termites and slender-jawed soil termites in temperate regions is from April to May; the swarming period of the Yunnan soil termites in tropical climates is from May to July; the swarming period of the yellow-winged big termites, Yunnan big termites, and soil ridge big termites in tropical climates is from May to July; the swarming period of the yellow-winged big termites in temperate climates is from June to July; and the swarming period of the small-headed hook termites and small termites in tropical climates is from April to May.
[0023] Preferably, the nest site of the whole-underground nest rearing termite is located at the high-illumination slope ditch side; the nest site of the above-ground earthen mound nest rearing termite is located on or around the earthen mound; the small nest rearing termite nest can produce a mature nest with a flying hole, which is often scattered around the large nest rearing termite nest.
[0024] Preferably, the soil humidity is measured by a soil humidity measuring device arranged on site.
[0025] Preferably, when adjusting the humidity, a water-saving sprinkler is used to spray the flying area, or a 1-2 meter long water accumulation groove is arranged on the top of the slope within 1 meter above the flying area, and clear water is slowly poured; when pouring wet soil, the speed is the best flow rate without forming a surface water flow.
[0026] Preferably, in step (b), the soil humidity after soaking is 12%-18%.
[0027] Preferably, in step (c), the incubation shed for the daytime flying rearing termite is a transparent shed; the incubation shed for the nighttime and dawn flying rearing termite is a semi-transparent or opaque shed.
[0028] Preferably, in step (d), the rain-following flying rearing termite includes the black-winged soil termite, the fine-jawed soil termite, the Yunnan soil termite, the earthen mound large termite and the Hainan soil termite.
[0029] The pressure-sensitive rain-following flying rearing termite includes the Yunnan large termite and the yellow-winged large termite.
[0030] Preferably, in step (d), it further includes pouring water through the flying area 1-2 days before flying after the rain, and then increasing the temperature to promote flying after the flying hole behavior appears.
[0031] Preferably, in step (e), the spectrum wavelength of the purple light lamp is 360-395 nm.
[0032] Preferably, the purple light lamp is an LED light source, the light is directly collected on the collection disc, and the surrounding scattered light is collected to induce the termite to crawl into the collection disc.
[0033] Preferably, a skirt around the collection disc is arranged to facilitate the termite to enter the collection disc.
[0034] Preferably, a clean paper crawling area is arranged around the collection disc to remove the soil and dust carried by the adult's feet and abdomen.
[0035] Compared with the prior art, the beneficial effects of the present application at least include:
[0036] The present application realizes artificial collection of reared termites by combining termite swarming behavior, microenvironment ecology principles, artificial simulation of temperature, humidity, light and pressure changes, and using the strong phototaxis of termite swarming adults to trap the flying termites through light rays. In addition, since termites have the risk of being infected with various pathogenic microorganisms during the process of flying out of the ground, ground crawling and flying, the collection method of the present application reduces the risk of pathogenic infection by disinfecting the swarming area, and finally achieves safe, efficient and clean collection of various reared termite adults. DETAILED DESCRIPTION
[0037] The technical solutions of the present application will be described in detail below with reference to the embodiments. The following embodiments are only used to more clearly illustrate the technical solutions of the present application, and therefore only serve as examples, but cannot limit the protection scope of the present application.
[0038] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in the present application should be understood as the usual meaning understood by the skilled person in the field to which the present application belongs.
[0039] Embodiment 1
[0040] The present embodiment is an artificial collection method for swarming adults of Odontotermes formosanus Shiraki, comprising the following steps:
[0041] In the Nanhua test base in Chuxiong, Yunnan, in April and May of each year, which is dry and less rainy every year, the ground slope ridge of the plot with rich termite nests is selected, the surface soil is removed, and the swarming area with 5-10 swarming holes is found 2-5m 2 A 1.5m long water tank is dug out on the top of the 0.5m high slope, 1 ton of clean water is slowly injected into the tank with a bucket within 2 days, and the soil area of 4m 2 When the swarming holes where there is no termite activity begin to appear termite activity and build swarming holes, a semi-transparent tarpaulin is used to build a 1m high temperature-increasing shed to cover the entire swarming hole area. When the termites start to open the top of the swarming hole, 0.05% sodium hypochlorite disinfectant is sprayed in the entire swarming hole area, a 25cm long and 35cm wide collection tray is buried, a 15W 395nm LED purple light lamp is erected directly above the tray, the position is such that the light can directly irradiate the collection tray, and a paper crawling belt is installed at the edge of the collection tray. The collection tray and the crawling belt are sprayed with the aforementioned disinfectant. When the termites completely open the swarming hole and the workers and soldiers appear, 5-30 minutes of water spraying is started to simulate light rain. When the termite adults appear and flow out of the swarming hole, the water spraying is stopped, the purple light lamp is turned on, and the artificially promoted termites are collected. A total of 100 nests of swarming areas are treated, and 0.1-2.5kg of adults per nest is obtained, which is 1-30 days earlier than the natural swarming period, 100% of the artificially promoted termites are collected in dry and hot sunny days, the collection period is 40 days, and the infection rate of the seedling is less than 0.1%.
[0042] Example 2
[0043] The present embodiment is a collection method of black-winged termites, comprising the following steps:
[0044] In Sichuan Neijiang, in the dry and rainy months of April and May every year, the ground slope ridge of the land block with abundant nests is selected, the surface soil is removed, and the number of flying holes in the flying area of 1-5m 2 , 0.5m above the top of the slope, a 1.5m long water tank is dug out, and 0.1 tons of water are slowly injected into the water tank within 2 days to soak the soil area of 4-8m 2 , the original flying hole without termite activity starts to appear termite activity and build flying holes, a semi-transparent shed cloth is built into a 1m high warming shed to cover the entire flying hole area, when the termites start to open the top of the flying hole, 0.05% sodium hypochlorite disinfectant is sprayed in the entire flying hole area, a 25cm long and 35cm wide collection tray is buried, a 15W 395nm LED purple light lamp is erected above the collection tray, the position is straightly irradiated by the light to collect the tray, and a paper crawling belt is installed at the edge of the collection tray. Spray the collection tray and the crawling belt with the disinfectant; when the termites completely open the flying hole and the workers and soldiers appear, start to spray water to simulate light rain for 5-10 minutes; when the adult termites flow out of the flying hole, stop spraying water, turn on the purple light, and start to collect the artificially promoted flying termites. A total of 100 nests of flying areas are treated, and 0.5-3.5kg of adult termites are obtained per nest, which is 1-30 days earlier than the natural flying period, 100% of the nests are artificially promoted to fly in dry and hot sunny days; the collection period is realized for 40 days, and the infection rate of the seedling production is less than 1%.
[0045] Example 3
[0046] The present embodiment is a collection method of black-winged termites, comprising the following steps:
[0047] In Yunnan Honghe, in the dry and rainy months of April and May every year, the ground slope ridge of the land block with abundant nests is selected, the surface soil is removed, and the number of flying holes in the flying area of 1-5m 2A 1.5-meter-long water trough was dug on the 0.5-meter-high slope above the site. 0.1 tons of clean water were slowly poured in over two days using buckets, soaking an area of 4-8 square meters. When termites began to appear and build swarming holes that were previously uninhabited, a 1-meter-high heating shed was erected using a semi-transparent cloth to cover the entire swarming hole area. When the termites began to open the top of the swarming holes, a 0.05% sodium hypochlorite disinfectant solution was sprayed throughout the swarming hole area. A 25cm-long and 35cm-wide collection tray was buried, and a 15W 395nm LED ultraviolet light was installed directly above the tray, positioned so that the light could directly illuminate the collection tray. Paper crawling strips were also installed along the edges of the collection tray. Spray the collection trays and crawling belts with the aforementioned disinfectant solution; when the termites have fully opened their swarming holes and worker and soldier termites appear to escort them, begin spraying water to simulate light rain for 5-10 minutes; when adult termites begin to emerge from the swarming holes, stop spraying water, turn on the ultraviolet light, and begin collecting the artificially induced winged adult termites. A total of 100 swarming areas were treated, yielding 0.5-3.5 kg of adult termites per nest, averaging 1-40 days earlier than the natural swarming period, achieving 100% artificial swarming during dry, hot, and sunny days without rain; achieving a 40-day collection period, with a seedling infection rate of <1%.
[0048] Example 4
[0049] This embodiment describes a method for collecting swarming adult black-winged subterranean termites, comprising the following steps:
[0050] In Honghe, Yunnan, during the dry months of April and May each year, the slopes and ridges of areas rich in ant nests are selected. The topsoil is removed, and swarming zones with 5-10 swarming holes are identified within a 1-5 meter radius. 2 A 1.5-meter-long water trough was dug on the top of a slope 0.5 meters above the slope. Then, 0.1 tons of clean water were slowly poured in using buckets over two days, soaking an area of 4-8 square meters of soil. 2 When termites begin to appear and construct swarming holes that were previously uninhabited, a 1-meter-high warming shed is erected using semi-transparent fabric, covering the entire swarming hole area. When the termites begin to open the top of the swarming holes, a 0.05% sodium hypochlorite disinfectant solution is sprayed throughout the area. A 25cm long and 35cm wide collection tray is buried, with a 15W 395nm LED ultraviolet light positioned directly above it, ensuring the light shines on the tray and its edge. A paper crawling strip is also installed. The collection tray and crawling strip are sprayed with the aforementioned disinfectant solution. When the termites have fully opened the swarming holes and worker and soldier termites appear to escort them, water is sprayed to simulate light rain for 5-10 minutes. When adult termites begin to emerge from the swarming holes, water spraying is stopped, the ultraviolet light is turned on, and the artificially induced winged adult termites are collected. A total of 100 swarming areas were treated, yielding 0.5-3.5 kg of adults per swarm, averaging 1-40 days earlier than the natural swarming period. Artificial swarming was achieved 100% under dry, hot, and sunny conditions without rain. The collection process took 40 days, with an infection rate of <1% in the produced seedlings.
[0051] Example 5
[0052] The present embodiment is a collection method of Yunnan soil termite alates, comprising the following steps:
[0053] In Xishuangbanna, Yunnan, in May, one month before the alate flight period, the ground slope ridge of the area with abundant nests is selected, the surface soil is removed, and the number of alate flight holes in the alate flight area of 2-5m 2 A 1.5-meter-long water groove is excavated on the top of the slope 0.5 meters above, and then 0.5 tons of water are injected into the water groove by the water injection method, and the soil area of 3m 2 When the alate flight holes originally without termite activity start to appear termite activity and build alate flight holes, a semi-transparent shed cloth is used to build a 1-meter-high temperature-increasing shed to cover the entire alate flight hole area. When the termites start to open the top of the alate flight hole, 0.05% sodium hypochlorite disinfectant is sprayed in the entire alate flight hole area, and a collection tray with a length of 25cm and a width of 35cm is buried. A 15W 395nm LED purple light lamp is installed above the collection tray, and the position is such that the light can directly shine on the collection tray. A paper crawling belt is installed at the edge of the collection tray. The collection tray and the crawling belt are sprayed with the aforementioned disinfectant. When the termites completely open the alate flight holes and the workers and soldiers appear, start to spray water to simulate light rain for 5-30 minutes. When the adult termites start to flow out of the alate flight holes, stop spraying water and turn on the purple light to start collecting artificially promoted alate termites. A total of 10 nests in the alate flight area are treated, and 2.0-10.5kg of adult termites per nest are obtained, which is 1-30 days earlier than the natural alate flight period, and 100% of the adult termites are artificially promoted in sunny days without rain. The collection period is 30 days, and the infection rate of the seedling is less than 1%.
[0054] Example 6
[0055] The present embodiment is a collection method of Yunnan soil termite alates, comprising the following steps:
[0056] In Xishuangbanna, Yunnan, in May, one month before the alate flight period, the ground slope ridge of the area with abundant nests is selected, the surface soil is removed, and the number of alate flight holes in the alate flight area of 2-5m 2When the originally inactive flier holes begin to show termite activity and build flier holes, a 1-meter-high temperature-increasing tent is built with a semi-transparent tent cloth covering the entire flier hole area. The next day after treatment, the tent is sealed and the air pump is appropriately pressurized. When the termites begin to open the top of the flier hole, 0.05% sodium hypochlorite disinfectant is sprayed in the entire flier hole area. A 25cm long and 35cm wide collection tray is buried, and a 15W 395nm LED purple light lamp is installed directly above the collection tray. The light is directed to the collection tray, and a paper crawling belt is installed at the edge of the collection tray. The collection tray and the crawling belt are sprayed with the aforementioned disinfectant. When the termites completely open the flier holes and worker ants and soldiers appear, the air pump is appropriately pressurized. When the adult termites emerge from the flier holes, the purple light is turned on, and the artificially promoted flying termites are collected. A total of 10 nests of flier areas were treated, and 4.0-11.5kg of adult termites were obtained per nest, which was 1-30 days earlier than the natural flier period, and 100% of the Yunnan Macrotermes termites were artificially promoted to fly on sunny days without rain. The collection period was 30 days, and the infection rate of the seedling production was less than 1%.
[0057] Example 7
[0058] The present embodiment is a method for collecting flying adult Macrotermes barneyi termites, comprising the following steps:
[0059] In Yunnan, one month before the flier period in May, the slope ridge of an area with abundant termite nests is selected, and the surface soil is removed. The number of flier holes is 5-30 per flier area of 2-5m 2 A 1.5m long water tank is dug on the top of the 0.5m high slope, and 1.0 tons of water are injected into the tank by spraying water to soak the soil area of 3m 2 When the originally inactive flier holes begin to show termite activity and build flier holes, a 1-meter-high temperature-increasing tent is built with a semi-transparent tent cloth covering the entire flier hole area. The next day after treatment, the tent is sealed and the air pump is appropriately pressurized. When the termites begin to open the top of the flier hole, 0.05% sodium hypochlorite disinfectant is sprayed in the entire flier hole area. A collection net bag is installed on the top of the temperature-increasing tent. The collection net bag is sprayed with the aforementioned disinfectant. When the termites completely open the flier holes and worker ants and soldiers appear, artificial rain is simulated by spraying water for 5-30 minutes, and the air pump is appropriately pressurized. When the adult termites emerge from the flier holes, the collection of artificially promoted flying termites is started. A total of 10 nests of flier areas were treated, and 1.0-2.5kg of adult termites were obtained per nest, which was 1-30 days earlier than the natural flier period, and 100% of the termites were artificially promoted to fly on sunny days without rain. The collection period was 30 days, and the infection rate of the seedling production was less than 1%.
[0060] Example 8
[0061] The present embodiment is a method for collecting flying adult Macrotermes barneyi termites, comprising the following steps:
[0062] At the Nanhua Experimental Base in Chuxiong, Yunnan, during the dry season of April and May each year, the slopes of areas rich in ant nests are selected, the topsoil is removed, and swarming zones with 5-10 swarming holes are identified within a 2-5 meter radius. 2 A 1.5-meter-long water trough was dug on the 0.5-meter-high slope above the site. One ton of clean water was slowly poured in over two days using buckets, soaking a 4-square-meter area of soil. When termites began to appear and construct swarming holes that were previously uninhabited, a 1-meter-high, semi-transparent shed was erected to cover the entire swarming hole area. When the termites began to open the top of the swarming holes, a 0.05% saturated ozone solution was sprayed throughout the area. A 25cm-long, 35cm-wide collection tray was buried, with a 15W 395nm LED UV lamp positioned directly above it, ensuring the light could directly illuminate the tray and its edges. A paper crawling strip was also installed. The collection tray and crawling strip were sprayed with the aforementioned disinfectant. When the termites had fully opened the swarming holes and worker and soldier termites appeared to escort them, simulated light rain was sprayed for 5-30 minutes. When adult termites emerge from the swarming holes, stop spraying water, turn on the UV light, and begin collecting the artificially induced winged adult termites. A total of 100 swarming areas were treated, yielding 0.1-2.5 kg of adults per nest, averaging 1-30 days earlier than the natural swarming period. Artificial swarming was achieved 100% on dry, hot, sunny days without rain. The collection process took 40 days, with an infection rate of <0.1% in the resulting seedlings.
[0063] Example 9
[0064] This embodiment describes a method for collecting swarming adults of the South China black-winged subterranean termite, including the following steps:
[0065] At the Nanhua Experimental Base in Chuxiong, Yunnan, during the dry season of April and May each year, the slopes of areas rich in ant nests are selected, the topsoil is removed, and swarming zones with 5-10 swarming holes are identified within a 2-5 meter radius. 2 A 1.5-meter-long water trough was dug at the top of the slope, which was 0.5 meters above the ground. Then, 1 ton of clean water was slowly poured into the trough over two days using buckets, soaking an area of 4 square meters of soil. 2When the originally inactive split flight holes begin to show termite activity and build split flight holes, a 1-meter-high temperature-increasing tent is set up with a semi-transparent tent cloth covering the entire split flight hole area. When the termites begin to open the top of the split flight hole, start spraying saturated ozone water disinfectant in the entire split flight hole area. A 25 cm long and 35 cm wide collection tray is buried, and a 15W 395nm LED ultraviolet light is installed directly above the tray. The light can directly irradiate the collection tray, and a paper crawling belt is installed on the edge of the collection tray. Spray the collection tray and the crawling belt with the aforementioned disinfectant. When the termites completely open the split flight hole and appear to be protecting the flight, start spraying water to simulate light rain for 5-30 minutes. When the adult termites appear to be flowing out of the split flight hole, stop spraying water and turn on the ultraviolet light to start collecting artificially promoted termites. A total of 100 nests of split flight areas were treated, and adult termites weighing 0.1-2.5 kg per nest were obtained, with an average of 1-30 days earlier than natural split flight period, 100% artificial promotion in dry and hot sunny days without rain. The collection period is 40 days, and the infection rate of seedling production is less than 0.1%.
[0066] Example 10
[0067] This embodiment is a method for collecting split flight adult termites of Odontotermes formosanus, comprising the following steps:
[0068] In the Yunnan Wenshan test base, in May of each year, select termite mounds of Odontotermes formosanus, remove the topsoil, and find split flight nests with 5-10 split flight holes. Slowly inject 1 ton of clean water into the entire nest area with a sprinkler, and soak the soil area of 4m 2 When the originally inactive split flight holes begin to show termite activity and build split flight holes, a 1-meter-high temperature-increasing tent is set up with a semi-transparent tent cloth covering the entire split flight hole area. When the termites begin to open the top of the split flight hole, start spraying 0.05% sodium hypochlorite disinfectant in the entire split flight hole area. A 25 cm long and 35 cm wide collection tray is buried, and a 15W 395nm LED ultraviolet light is installed directly above the tray. The light can directly irradiate the collection tray, and a paper crawling belt is installed on the edge of the collection tray. Spray the collection tray and the crawling belt with the aforementioned disinfectant. When the termites completely open the split flight hole and appear to be protecting the flight, start spraying water to simulate light rain for 5-30 minutes. When the adult termites appear to be flowing out of the split flight hole, stop spraying water and turn on the ultraviolet light to start collecting artificially promoted termites. A total of 10 nests of split flight areas were treated, and adult termites weighing 0.5-2.5 kg per nest were obtained, with an average of 1-30 days earlier than natural split flight period, 100% artificial promotion in dry and hot sunny days without rain. The collection period is 20 days, and the infection rate of seedling production is less than 0.1%.
[0069] Example 11
[0070] This embodiment is a method for collecting split flight adult termites of Hainan termite, comprising the following steps:
[0071] In the Yunnan Honghe test base, in each year of drought and little rain in each year from April to May, the ground side slope ridge of the ant nest rich plot is selected, the surface soil is removed, the number of 2-5m 2 , the top of 0.5m above is excavated to 1.5m long water tank, then 1 ton of water is slowly injected into the water bucket within 2 days, the area of 4m2 of the soil is infiltrated, the ant activity starts to appear in the original no-termite activity of the flying hole and the flying hole is built, the semi-transparent shed cloth is built to 1m high warming shed, covering the entire flying hole area, when the termite starts to open the flying hole top, the 0.05% sodium hypochlorite disinfectant is sprayed in the entire flying hole area, the 25cm long and 35cm wide collection tray is buried, the 15W 365nm LED purple light lamp is erected above the collection tray, the position is straight to the collection tray, the paper crawling belt is installed at the edge of the collection tray. The collection tray and the crawling belt are sprayed with the disinfectant. When the termite completely opens the flying hole and the worker and soldier ants appear, the water is sprayed to simulate light rain for 5-30 minutes. When the adult termites appear to flow out of the flying hole, stop spraying water, turn on the purple light, and start collecting artificially promoted flying termites. A total of 30 nests of flying holes are treated, and 0.5-1.5kg of adult insects per nest is obtained, which is 1-30 days earlier than the natural flying period, and 100% of the artificially promoted flying is realized in the rainless dry and hot sunny days. The collection period is realized for 40 days, and the seedling infection rate is less than 0.1%.
[0072] Example 12
[0073] The present embodiment is a method for collecting flying adult termites of Microcerotermes diversus, comprising the following steps:
[0074] In Xishuangbanna, Yunnan, in April one month before the flying period, the ground side slope ridge of the ant nest rich plot is selected, the surface soil is removed, the number of 2-5m 2 , the top of 0.5m above is excavated to 1.5m long water tank, then 0.1 ton of water is slowly injected into the water bucket within 2 days, the area of 3m 2When the termite activity begins to appear in the original non-termite activity branch hole and the branch hole is built, a 1-meter-high temperature shed is built with a translucent shed cloth, covering the entire branch hole area. When the termite begins to open the top of the branch hole, 0.05% sodium hypochlorite disinfectant is sprayed in the entire branch hole area. A 25cm long and 35cm wide collection tray is buried, and a 15W 395nm LED purple light lamp is installed above the tray. The light can directly irradiate the collection tray. A paper crawling belt is installed at the edge of the collection tray. The collection tray and the crawling belt are sprayed with the aforementioned disinfectant. When the termite completely opens the branch hole and the worker and soldier ants appear, start spraying water to simulate light rain for 5-30 minutes. When the adult termites appear and flow out of the branch hole, stop spraying water and turn on the purple light to start collecting artificially promoted termites. A total of 10 nests of branch holes are treated, and 0.01-0.2kg of adult termites are obtained per nest, which is 1-30 days earlier than the natural branch flying period, and 100% of the artificial promotion is achieved in sunny days without rain. The collection period is 30 days, and the infection rate of the seedling production is less than 1%.
[0075] Example 13
[0076] The present embodiment is a method for collecting termite branch flying adults, comprising the following steps:
[0077] In Xishuangbanna, Yunnan, in April, one month before the branch flying period, choose an area with abundant termite nests, remove the surface soil, and find 2-5m 2 A 1.0-meter-long water tank is dug on the top of the 0.5-meter-high slope, and 0.1 tons of water are injected into the tank by spraying water, soaking an area of 2m 2 When the termite activity begins to appear in the original non-termite activity branch hole and the branch hole is built, a 1-meter-high temperature shed is built with a translucent shed cloth, covering the entire branch hole area. When the termite begins to open the top of the branch hole, 0.05% sodium hypochlorite disinfectant is sprayed in the entire branch hole area. A 25cm long and 35cm wide collection tray is buried, and a 15W 395nm LED purple light lamp is installed above the tray. The light can directly irradiate the collection tray. A paper crawling belt is installed at the edge of the collection tray. The collection tray and the crawling belt are sprayed with the aforementioned disinfectant. When the termite completely opens the branch hole and the worker and soldier ants appear, start spraying water to simulate light rain for 5-30 minutes. When the adult termites appear and flow out of the branch hole, stop spraying water and turn on the purple light to start collecting artificially promoted termites. A total of 10 nests of branch holes are treated, and 0.01-0.2kg of adult termites are obtained per nest, which is 1-30 days earlier than the natural branch flying period, and 100% of the artificial promotion is achieved in sunny days without rain. The collection period is 30 days, and the infection rate of the seedling production is less than 1%.
[0078] Example 14
[0079] The present embodiment is a method for collecting branch flying adults of tropical yellow-winged termites, comprising the following steps:
[0080] In Xishuangbanna, Yunnan, in May, one month before the flying period, select the nest flying area, remove the topsoil, find the flying hole number in the flying nest of 5-30, then inject 0.5 tons of clean water into the soil, and immerse the soil area of 4m 2 When the flying hole starts to appear termite activity and build flying holes, build a 1-meter-high temperature-increasing shed with a translucent shed cloth covering the entire flying hole area. The next day after treatment, the shed is sealed and the air pump is appropriately pressurized. When the termites start to open the flying hole top, start spraying 0.05% sodium hypochlorite disinfectant in the entire flying hole area. A 25cm long and 35cm wide collection tray is buried, with a 15W 395nm LED ultraviolet light lamp installed directly above the collection tray. The light can be directly collected in the tray, and a paper crawling belt is installed on the edge of the collection tray. Spray the collection tray and crawling belt with the disinfectant. When the termites completely open the flying hole and appear worker ants and soldiers, continue to seal the shed and appropriately pressurize the air pump. When adult termites appear outside the flying hole, stop spraying water and turn on the ultraviolet light to start collecting artificially promoted flying termites. A total of 10 nests of flying area were treated, and 4.0-11.5kg of adult termites were obtained per nest, with an average of 1-30 days earlier than the natural flying period, and 100% of the Yunnan Macrotermes realized artificial flying in sunny days. A 30-day collection period was realized, and the seedling infection rate was <0.1%.
[0081] Example 15
[0082] The present embodiment is a method for collecting flying termites of tropical Macrotermes, comprising the following steps:
[0083] In Xishuangbanna, Yunnan, in May, one month before the flying period, select the nest flying area, remove the topsoil, find the flying hole number in the flying nest of 5-30, then inject 0.5 tons of clean water into the soil, and immerse the soil area of 4m 2, originally no termite activity starts to appear when the half-transparent shed cloth is built 1 meter high, and the shed cloth is covered with the whole area of the termite nest. The next day, the shed is sealed with mud, and the air pump is appropriately pressurized. When the termites start to open the top of the nest, start spraying 0.05% sodium hypochlorite disinfectant in the whole area of the nest. A 25cm long and 35cm wide collection tray is buried, and a 15W 395nm LED ultraviolet light is installed above the tray. The light can directly irradiate the collection tray, and a paper crawling belt is installed around the edge of the collection tray. Spray the collection tray and the crawling belt with the disinfectant. When the termites completely open the nest and appear worker ants and soldiers, continue to seal the shed and appropriately pressurize the air pump. When the adult termites appear outside the nest, stop spraying water and turn on the ultraviolet light to start collecting artificially promoted flying termites. A total of 10 nests were treated, and 4.0-11.5kg of adult termites were obtained per nest, which was 1-30 days earlier than the natural flying period, and 100% of the Yunnan Macrotermes natalensis termites were artificially promoted to fly on sunny days without rain. The collection period was 30 days, and the infection rate of the seedling was less than 0.1%.
[0084] Example 16
[0085] The present embodiment is a method for collecting flying adult termites for food, comprising the following steps:
[0086] The large-scale termites of the genus Odontotermes and Macrotermes were collected by the high-efficiency flying method of termites in the foregoing Example 10, and environmental disinfection measures were used throughout the collection process. A total of 200 nests were treated, and 1.0-10.5kg of adult termites were obtained per nest, which was 1-30 days earlier than the natural flying period, and 100% of the Yunnan Macrotermes natalensis termites were artificially promoted to fly on sunny days without rain. The collection period was 30 days, and the infection rate of the seedling was less than 0.1%.
[0087] Comparative Example 1
[0088] The present embodiment is a method for collecting flying adult termites for food, comprising the following steps:
[0089] The large-scale termites of the genus Odontotermes and Macrotermes were collected by the high-efficiency flying method of termites in the foregoing Example 10, and environmental disinfection measures were used throughout the collection process. A total of 200 nests were treated, and 1.0-10.5kg of adult termites were obtained per nest, which was 1-30 days earlier than the natural flying period, and 100% of the Yunnan Macrotermes natalensis termites were artificially promoted to fly on sunny days without rain. The collection period was 30 days, and the infection rate of the seedling was less than 0.1%.
[0090] Comparative Example 2
[0091] The present comparative example is a collection method of dispersing adults of Odontotermes formosanus, comprising the following steps:
[0092] In Honghe, Yunnan, in the dry and rainy months of April to May every year, the ground slope ridge of the land block with rich ant nests is selected, the surface soil is removed, and the dispersing area of 1-5 m 2 , 0.5 meters above the top of the slope is excavated to form a 1.5-meter-long water tank, and 0.1 tons of clean water are slowly injected into the tank within 2 days to infiltrate the soil area of 4-8 m 2 , When the dispersing hole originally without termite activity starts to appear termite activity and build dispersing hole, a semi-transparent shed cloth is used to build a 1-meter-high temperature-increasing shed to cover the entire dispersing hole area. When the termites start to open the top of the dispersing hole, stop spraying disinfectant liquid in the entire dispersing hole area, bury a collection tray with a length of 25 cm and a width of 35 cm, and a 15W 395nm LED purple light lamp is installed above the collection tray. The light can directly irradiate the collection tray, and a paper crawling belt is installed at the edge of the collection tray. When the termites completely open the dispersing hole and appear worker ants and soldier ants, start to spray water to simulate light rain for 5-10 minutes. When the adult termites appear to flow out of the dispersing hole, stop spraying water and turn on the purple light to start collecting artificially promoted termite alates. A total of 100 nests of dispersing areas are treated, and 0.5-3.5 kg of adult termites are obtained per nest, which is 1-40 days earlier than the natural dispersing period, and 100% of the artificially promoted flying is realized in dry and hot sunny days without rain. The collection period is 40 days, and the infection rate of seedling production is 30%.
[0093] Comparative Example 3
[0094] The present comparative example is a collection method of dispersing adults of Odontotermes formosanus, comprising the following steps:
[0095] In Nanhua, Yunnan, in the dry and rainy months of April to May every year, the ground slope ridge of the land block with rich ant nests is selected, the surface soil is removed, and the dispersing area of 1-5 m 2 , without water injection, and water spraying to simulate light rain for 180 minutes. A total of 10 nests of dispersing areas are treated, and no adult termites are obtained.
[0096] Comparative Example 4
[0097] The present comparative example is a collection method of dispersing adults of Odontotermes formosanus, comprising the following steps:
[0098] In Honghe, Yunnan, in the dry and rainy months of April to May every year, the ground slope ridge of the land block with rich ant nests is selected, the surface soil is removed, and the dispersing area of 1-5 m 2 , without water injection, and water spraying to simulate light rain for 360 minutes. A total of 10 nests of dispersing areas are treated, and no adult termites are obtained.
[0099] Comparative Example 5
[0100] The present comparative example is a collection method of flying adults of black-winged soil termites, comprising the following steps:
[0101] In Neijiang, Sichuan, in the dry and less rainy months of April and May each year, the ground slopes of plots with abundant termite nests are selected, the surface soil is removed, and the number of flying holes of 1-5m 2 , without water, water is sprayed to simulate light rain for 360 minutes. A total of 10 nests of flying areas are treated, and 0.5kg of any adult per nest is obtained. The average is 1-40 days earlier than the natural flying period, and 100% of the artificial flying is achieved in dry and hot sunny days. The collection period is realized for 40 days, and the infection rate of seedling production is 70%.
[0102] Comparative Example 6
[0103] The present comparative example is a collection method of flying adults of edible termites, comprising the following steps:
[0104] In the natural flying season of termites, soil termites and large termite flying adults are captured by light and picked up in the environment, a total of 20 nests of flying areas are treated, and 0.1-1.5kg of adults per nest is obtained. Using edible termite raw materials, according to the standard pathogen detection method, the detection indexes of various pathogens are much higher than the food safety standard, and it is not suitable for eating.
[0105] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application, and they should be covered in the scope of the claims and the specification of the present application.
Claims
1. A method for artificially collecting reproductives of a reared termite, characterized by, It comprises the following steps: (a) determination of the swarming area: During the swarming period of the reared termites, the mud nests of the termites are found around the swarming area, the surface soil is removed, the flat round and fan-shaped holes are found, and the swarming area of the reared termites is determined; (b) humidity adjustment: In 1~3 days of the swarming period of the breeding termite, 0.5~2t of clean water is used to soak the soil within 2~10m around the swarming area 2 range; (c) temperature and light control: After the humidity is adjusted, when the termites are found to be active and build swarming holes in the swarming area, a heat preservation shed is built to cover the swarming area, and the ground surface temperature in the heat preservation shed is controlled to be 17-29℃; (d) swarming stimulation: When the reared termites start to build swarming holes and prepare to swarm, the reared termites swarming after rain and after the rain are collected, light rain is simulated in the heat preservation shed to stimulate swarming; the reared termites swarming after the rain and after the pressure-sensitive weather clear up are collected, the heat preservation shed is sealed and pressurized to stimulate swarming; (e) light-induced collection: When the reared termites swarm at night, a purple light collecting tray is set on one side of the swarming area before the adult termites appear in the swarming holes and start to swarm out of the swarming holes, so as to collect the swarming adult termites; When the reared termites swarm during the day, a collecting net is set on the top of the heat preservation shed to collect the swarming adult termites according to the habit of the adult termites flying upward to light; In the step (d), disinfectant is also sprayed on the whole swarming area; In the step (a), the swarming period of the reared termites is as follows: The swarming period of the blackish-winged soil termites, Hainan soil termites and fine-jawed soil termites in tropical climate is from February to April; the swarming period of the blackish-winged soil termites and fine-jawed soil termites in temperate climate is from April to May; the swarming period of the Yunnan soil termites in tropical climate is from May to July; the swarming period of the yellow-winged big termites, Yunnan big termites and soil ridge big termites in tropical climate is from May to July; the swarming period of the yellow-winged big termites in temperate climate is from June to July; the swarming period of the small-headed hook termites and small termites in tropical climate is from April to May; In the step (c), the heat preservation shed for the reared termites swarming during the day is a transparent shed; the heat preservation shed for the reared termites swarming at night and during the dawn and dusk is a semi-transparent or opaque shed; In the step (d), the reared termites swarming after rain and after the rain include the blackish-winged soil termites, fine-jawed soil termites, Yunnan soil termites, soil ridge big termites and Hainan soil termites; The reared termites swarming after the pressure-sensitive weather clear up include the Yunnan big termites and yellow-winged big termites.
2. The method for artificial collection of reared termite alates according to claim 1, characterized in that, In the step (d), when the reared termites swarming after the weather clear up are collected, the swarming area is poured thoroughly 1-2 days before swarming, and then the temperature is increased to stimulate swarming after the swarming holes are built.
3. The method for artificial collection of reared termite alates according to claim 1, characterized in that, In the step (e), the spectrum wavelength of the purple light is 360-395nm.
Citation Information
Patent Citations
Method for collecting winged adult termites
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Termite flying winged adult collecting device
CN210901042U