PCR automation all-in-one machine

Abstract

The application relates to a PCR automation all-in-one machine, which comprises a rack, a nucleic acid amplification module and a nucleic acid detection module arranged in the rack, the nucleic acid amplification module is used for amplifying a reaction sample, and the nucleic acid detection module is used for detecting the reaction sample. A kit carrying the reaction sample is placed in the nucleic acid amplification module for amplification, and after amplification, detection is carried out in the nucleic acid detection module, and the detection process is simple. One kit can enter the PCR automation all-in-one machine for detection, and a large number of samples do not need to be collected for unified detection, and a detection result can be quickly given. The nucleic acid amplification module and the nucleic acid detection module occupy space, thereby improving the compactness of the structure of the PCR automation all-in-one machine and reducing the space occupied by the equipment. The configuration, transfer, amplification and detection of the reaction sample can be automatically completed, so that professional testers are not needed to operate, the operation is simple, and the professional requirement degree of PCR detection is low. Moreover, all the modules are arranged in the machine shell, so that aerosols and the like are prevented from escaping to cause pollution.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of in vitro diagnosis, and particularly relates to a PCR automatic integrated machine. BACKGROUND

[0002] PCR refers to a molecular biology experimental method for synthesizing specific DNA fragments in vitro, which is mainly composed of three steps of high-temperature denaturation, low-temperature annealing and appropriate temperature extension, which are repeatedly cycled. Before PCR amplification, the reaction sample needs to be placed in a carrier, wherein the reaction sample is composed of collected throat swabs or nasal swabs and reagents for PCR amplification. During PCR amplification, the reaction sample needs to be heated by a heater and cooled by a cooling mechanism, so that the reaction sample is cycled in the stages of high-temperature denaturation, low-temperature annealing and appropriate temperature extension.

[0003] The PCR detection equipment in the prior art needs to be operated by professional testers to form a reaction sample, and the operation is complex, and the PCR detection requires high professional degree.

[0004] In order to avoid aerosol pollution, the equipment needs to be placed in a special room, and the PCR detection equipment has high environmental requirements.

[0005] The structure of the PCR detection equipment is not compact, and the equipment occupies a large space. In addition, a large number of samples need to be collected and detected uniformly, and the detection result cannot be given quickly. SUMMARY

[0006] The present application aims to provide a PCR automatic integrated machine to solve one of the above technical problems.

[0007] To achieve the above purpose, the present application provides a PCR automatic integrated machine, which comprises a rack, a nucleic acid amplification module and a nucleic acid detection module arranged on the rack, the nucleic acid amplification module is used for amplifying a reaction sample, and the nucleic acid detection module is used for detecting the reaction sample.

[0008] Optionally, the PCR automatic integrated machine further comprises a preparation module, the preparation module is arranged on the rack and is used for preparing a sample and a reagent into a reaction sample.

[0009] Optionally, the preparation module comprises a carrying sub-module and a pipetting sub-module arranged on the upper side of the carrying sub-module, the carrying sub-module and the pipetting sub-module can move relative to each other, so that the pipetting sub-module transfers the solution on the carrying sub-module and injects the reaction sample into a reagent box.

[0010] Optionally, the pipetting sub-module comprises a pipette, and at least one of the pipette and the carrying sub-module is movable in a horizontal plane, and at least one of the pipette and the carrying sub-module is movable in a vertical direction.

[0011] Optionally, the carrying sub-module is capable of carrying a reagent box, and the pipetting sub-module is used to transfer a solution between cavities of the reagent box.

[0012] Optionally, the carrying sub-module comprises a bracket, and the bracket is provided with a receiving portion for receiving the reagent box.

[0013] Optionally, the receiving portion comprises a plurality of receiving grooves, and a reagent carrying portion of the reagent box is capable of being inserted into the receiving grooves.

[0014] Optionally, at least one of the plurality of receiving grooves is provided with a heating structure for heating a reaction sample in the reagent carrying portion.

[0015] Optionally, the PCR automation all-in-one machine further comprises a transfer module arranged in the rack, and the transfer module is used to transfer the reagent box or the carrier between the carrying sub-module and the nucleic acid amplification module, or between the carrying sub-module, the nucleic acid amplification module and the nucleic acid detection module.

[0016] Optionally, the PCR automation all-in-one machine further comprises a cutting module arranged in the rack, and the cutting module is used to cut the reagent box between the carrier of the reagent box and the reagent carrying portion of the reagent box.

[0017] Optionally, the PCR automation all-in-one machine further comprises a turnover module arranged in the rack, and the turnover module is used to turn over the carrier of the reagent box by a preset angle.

[0018] Optionally, the PCR automation all-in-one machine further comprises a bending module arranged in the rack, and the bending module is used to bend one end of the reagent box by a preset angle, or the bending module is used to bend the carrier of the reagent box by a preset angle and break the reagent box between the carrier and the reagent carrying portion.

[0019] Optionally, the reaction sample is contained in the carrier, and the carrier is a flat structure, and the nucleic acid amplification module and / or the nucleic acid detection module vertically insert the carrier.

[0020] Optionally, the PCR automation all-in-one machine further comprises a control module for controlling the nucleic acid amplification module and the nucleic acid detection module.

[0021] Optionally, the nucleic acid detection module comprises a fluorescence detection optical path system, and the fluorescence detection optical path system comprises:

[0022] at least one fluorescence emission unit, configured to emit excitation light.

[0023] Optionally, the number of the fluorescence emission units is at least two, and the control module controls the at least two fluorescence emission units to emit excitation light in different time periods respectively.

[0024] Optionally, the fluorescence detection optical path system further comprises:

[0025] a fluorescence detection unit, comprising at least two fluorescence transmission optical paths, and the at least two fluorescence emission units correspond to the at least two fluorescence transmission optical paths one by one.

[0026] Optionally, the fluorescence detection unit further comprises a detector, and the plurality of fluorescence transmission optical paths are connected to the detector.

[0027] Optionally, the number of the detector is one, and the detector is electrically connected to the control module to record the intensity of the fluorescence signal in time periods.

[0028] Optionally, the fluorescence detection optical path system further comprises a fiber seat, the fluorescence transmission optical path comprises a collection optical fiber, the emission end of the excitation optical fiber emits the excitation light, and the emission end of the collection optical fiber emits the fluorescence signal to form an optical fiber group, the optical fiber group is arranged in the fiber seat, the optical fibers in the fiber seat are arranged along a preset direction, the preset direction is the radial direction of the optical fibers, so that the emission end of the collection optical fiber and the emission end of the excitation optical fiber are arranged flatly in the fiber seat.

[0029] Optionally, the at least two fluorescence transmission optical paths and the at least two fluorescence emission units form at least two groups of the optical fiber group, and the at least two groups of the optical fiber group are arranged along the preset direction in sequence.

[0030] Optionally, one optical fiber group comprises at least two collection optical fibers, and at least one collection optical fiber is arranged on both sides of the preset direction of the excitation optical fiber in one optical fiber group.

[0031] Optionally, the nucleic acid amplification module comprises at least one temperature adjusting mechanism, and the temperature adjusting mechanism can change the temperature of the reaction sample in the carrier.

[0032] Optionally, the nucleic acid amplification module comprises two groups of temperature adjusting mechanisms, and the two groups of temperature adjusting mechanisms are a first temperature adjusting mechanism and a second temperature adjusting mechanism respectively, the positions of the first temperature adjusting mechanism and / or the second temperature adjusting mechanism are adjustable, so as to be close to or away from each other, and the carrier is clamped between the first temperature adjusting mechanism and the second temperature adjusting mechanism.

[0033] Optionally, the nucleic acid amplification module comprises a driving mechanism, which drives the first temperature adjusting mechanism and / or the second temperature adjusting mechanism to move closer to or away from each other.

[0034] Optionally, a plurality of elastic leveling components are arranged between the driving mechanism and the first temperature adjusting mechanism and / or the second temperature adjusting mechanism.

[0035] Optionally, the nucleic acid amplification module further comprises a pressing mechanism, and the first temperature adjusting mechanism and / or the second temperature adjusting mechanism is connected with the pressing mechanism to press the pressing cavity of the carrier clamped between the first temperature adjusting mechanism and the second temperature adjusting mechanism.

[0036] Optionally, a stepped mounting hole is arranged on the first temperature adjusting mechanism and / or the second temperature adjusting mechanism, the pressing mechanism comprises a pressing component and a buffer, the pressing component is connected to the stepped mounting hole, and the buffer is connected between the pressing component and the stepped mounting hole to enable the pressing component to elastically contact the carrier.

[0037] Optionally, the first temperature adjusting mechanism and the second temperature adjusting mechanism each comprise a cooling component for cooling the carrier.

[0038] Optionally, the first temperature adjusting mechanism and the second temperature adjusting mechanism each further comprise a heater, and the heater is arranged on the side of the cooling component of the first temperature adjusting mechanism and the second temperature adjusting mechanism that is closer to each other.

[0039] Optionally, the heater comprises a heating component, and the PCR automatic integrated machine further comprises a resistance detection component for detecting the resistance of the heating component.

[0040] Optionally, the PCR automatic integrated machine further comprises a first temperature detection unit for detecting the temperature of the heater.

[0041] Optionally, the nucleic acid amplification module further comprises a positioning mechanism for positioning the carrier, and the positioning mechanism is connected to the first temperature adjusting mechanism or the second temperature adjusting mechanism.

[0042] Optionally, the flat structure refers to that the ratio of the size of the carrier in the direction perpendicular to the thickness direction of the carrier to the size of the carrier in the thickness direction is greater than 5:1.

[0043] Optionally, the ratio is 50:1 to 100:1.

[0044] As can be seen from the above, the technical solution provided by this invention involves placing a reagent kit containing the reaction sample into the nucleic acid amplification module for amplification, followed by detection in the nucleic acid detection module, resulting in a simple detection process. A single reagent kit can be used in an automated PCR machine, eliminating the need for collecting large quantities of samples for unified testing and providing rapid results. The nucleic acid amplification and detection modules occupy little space, thus improving the compactness of the automated PCR machine and reducing its footprint. The system determines the type of fluorescence signal based on the time period, resulting in low equipment cost, a simple structure, no mechanical switching, and fast detection speed. The preparation, transfer, amplification, and detection of the reaction sample can all be completed automatically, eliminating the need for professional testing personnel. The operation is simple, and the professional requirements for PCR detection are low. Furthermore, all modules are housed within the machine casing, preventing the escape of aerosols and other contaminants. Therefore, even if the automated PCR machine is not placed in a dedicated room, it will not cause contamination. Attached Figure Description

[0045] Figure 1a This is a front view of the PCR automated integrated machine provided in this embodiment of the invention;

[0046] Figure 1b This is a perspective view of the PCR automated integrated machine provided in the embodiments of the present invention;

[0047] Figure 2a This is a schematic diagram of the structure of the reagent kit provided in an embodiment of the present invention;

[0048] Figure 2b This is a cross-sectional view of the carrier provided in an embodiment of the present invention;

[0049] Figure 2c This is a structural schematic diagram of the carrier provided in another embodiment of the present invention;

[0050] Figure 3 This is a schematic diagram of the structure of a partially automated PCR integrated machine provided in an embodiment of the present invention;

[0051] Figure 4 This is a schematic diagram of the PCR automated integrated machine for preparing reaction samples provided in this embodiment of the invention;

[0052] Figure 5a This is a schematic diagram of the bracket provided in an embodiment of the present invention;

[0053] Figure 5b This is a schematic diagram of another bracket provided in an embodiment of the present invention;

[0054] Figure 6a This is a schematic diagram of the structure of the vector inserted into the nucleic acid amplification module according to an embodiment of the present invention;

[0055] Figure 6bis another structure schematic view of the nucleic acid amplification module when the carrier is inserted according to the embodiment of the present application;

[0056] Figure 7a is a structure schematic view of another cutting module according to the embodiment of the present application;

[0057] Figure 7b is a perspective view of another PCR automatic integrated machine according to the embodiment of the present application;

[0058] Figure 7c is Figure 7b is an enlarged view of B in figure 16;

[0059] Figure 8a is a structure schematic view of a first bending module according to the embodiment of the present application;

[0060] Figure 8b is a structure schematic view of a second bending module according to the embodiment of the present application;

[0061] Figure 8c is a front view of another PCR automatic integrated machine according to the embodiment of the present application;

[0062] Figure 9 is a structure schematic view of the carrier, the nucleic acid amplification module and the nucleic acid detection module according to the embodiment of the present application;

[0063] Figure 10 is a structure schematic view of a nucleic acid detection module and a nucleic acid amplification module (the first temperature adjusting mechanism and the second temperature adjusting mechanism do not clamp the carrier) according to the embodiment of the present application;

[0064] Figure 11 is a top view of a nucleic acid detection module and a nucleic acid amplification module in a normal use state according to the embodiment of the present application;

[0065] Figure 12 is a structure schematic view of another nucleic acid amplification module according to the embodiment of the present application;

[0066] Figure 13 is Figure 12 is an exploded view of the nucleic acid amplification module in figure 18;

[0067] Figure 14 is Figure 12 is a sectional view of the first position of the nucleic acid amplification module in figure 19;

[0068] Figure 15 is a structure schematic view of the cooling main body according to the embodiment of the present application;

[0069] Figure 16 is Figure 12 is a sectional view of the second position of the nucleic acid amplification module in figure 20;

[0070] Figure 17 is Figure 12 is a sectional view of a third position of the nucleic acid amplification module;

[0071] Figure 18 is Figure 12 is a sectional view of a fourth position of the nucleic acid amplification module;

[0072] Figure 19 is Figure 18 is a partial enlarged view of A;

[0073] Figure 20 is a structural schematic diagram of another nucleic acid amplification module provided by an embodiment of the present application;

[0074] Figure 21 is a structural schematic diagram of another nucleic acid amplification module provided by an embodiment of the present application;

[0075] Figure 22 is a structural schematic diagram of a carrier, a bearing plate and a positioning mechanism provided by an embodiment of the present application;

[0076] Figure 23 is a structural schematic diagram of a bearing plate and a positioning mechanism provided by an embodiment of the present application;

[0077] Figure 24a is a structural schematic diagram of a nucleic acid amplification module provided by an embodiment of the present application;

[0078] Figure 24b is a structural schematic diagram of a bearing plate provided by an embodiment of the present application;

[0079] Figure 25 is a temperature-time curve of a carrier provided by an embodiment of the present application;

[0080] Figure 26 is a structural schematic diagram of a heater provided by an embodiment of the present application;

[0081] Figure 27 is a structural schematic diagram of a nucleic acid detection module and a carrier provided by an embodiment of the present application;

[0082] Figure 28 is a structural schematic diagram of a fluorescence detection light path in the prior art;

[0083] Figure 29 is a structural schematic diagram of a fluorescence detection unit provided by the present application;

[0084] Figure 30 is a structural schematic diagram of another fluorescence detection unit provided by an embodiment of the present application;

[0085] Figure 31is a structural schematic view of a fiber seat provided by an embodiment of the present application;

[0086] Figure 32 is a structural schematic view of another view of a fiber seat provided by an embodiment of the present application;

[0087] Figure 33 is a structural schematic view of a nucleic acid detection module detecting from one side of a carrier provided by an embodiment of the present application;

[0088] Figure 34 is a structural schematic view of a nucleic acid detection module detecting from two sides of a carrier provided by an embodiment of the present application.

[0089] In the figure:

[0090] 1, fluorescence emission unit; 11, excitation optical fiber; 12, light source;

[0091] 2, fluorescence detection unit; 21, fluorescence transmission optical path; 211, optical filter; 212, collection optical fiber; 213, collimating lens; 22, detector; 23, turntable;

[0092] 4, carrier; 41, amplification cavity; 42, side wall; 43, first wall; 44, second wall; 45, bending notch; 46, extrusion cavity;

[0093] 51, elastic leveling assembly; 511, connecting rod; 512, elastic member; 513, first flange;

[0094] 52, first temperature adjusting mechanism;

[0095] 521, cooling assembly; 5211, cooling main body; 52111, cooling shell; 52112, cooling fin; 52113, cooling groove; 52116, medium port; 52117, temperature measurement hole; 52118, temperature measurement protrusion; 5212, base; 5214, electric connection line; 5215, heat preservation layer; 5216, second temperature detection unit; 5217, cover body; 5218, first sealing ring; 5219, second sealing ring; 2013, seat body; 2014, flat groove;

[0096] 522, heater; 523, stepped mounting hole; 5231, first stepped surface; 5232, second stepped surface; 524, adapter plate;

[0097] 53, driving mechanism; 531, driving member; 532, mounting frame; 533, screw rod; 534, nut; 535, driving gear; 536, driven gear; 537, bearing;

[0098] 54, guide mechanism; 541, guide rail; 542, sliding block;

[0099] 55, support seat;

[0100] 56. The second temperature adjusting mechanism; 561. The bearing plate; 5611. The through hole;

[0101] 57. The positioning mechanism; 5711. The first positioning part; 5712. The second positioning part; 5713. The first plate; 5714. The second plate; 572. The second positioning assembly; 5721. The fixing part; 5722. The elastic adjusting part; 5723. The abutting part; 5724. The chamfer;

[0102] 58. The extruding mechanism; 581. The buffer; 582. The mounting rod; 583. The pressure head; 584. The second flange;

[0103] 91. The heating member; 92. The upper conducting assembly; 921. The uniform heating layer; 922. The insulation layer; 93. The temperature calibration part; 94. The rapid conducting part; 941. The patch; 942. The guide column; 95. The lower conducting assembly; 951. The insulation thermal resistance layer; 952. The thermal conducting layer; 96. The wiring port; 97. The external electrical connection contact; 98. The electrical connection lead; 99. The first temperature detecting unit;

[0104] 10. The nucleic acid amplification module;

[0105] 20. The nucleic acid detection module; 201. The fluorescence detection optical path system; 2011. The optical fiber set; 2012. The optical fiber seat;

[0106] 30. The preparation module; 31. The bearing sub-module; 311. The bracket; 3111. The accommodating groove; 3112. The heating structure; 3113. The groove wall;

[0107] 32. The pipetting sub-module; 321. The pipetting gun; 322. The vertical driving member; 323. The horizontal driving member; 324. The pipetting connecting member; 325. The pipetting gear; 326. The rack;

[0108] 40. The machine frame;

[0109] 50. The control module;

[0110] 60. The transfer module; 61. The taking and placing member; 66. The vertical transfer driving member; 67. The horizontal transfer driving member; 68. The transfer gear; 69. The transfer connecting member;

[0111] 70. The cutting module; 701. The cutting driving assembly; 702. The second cutter; 703. The first cutter;

[0112] 80. The bending module; 801. The pressing column; 802. The horizontal driving member; 803. The vertical driving member; 804. The second roller; 805. The first roller; 806. The handle; 807. The pressing column lifting driving part; 808. The bending driving structure;

[0113] 90, recovery module;

[0114] 7, turnover module; 71, turnover driving member; 72, first plug-in member; 73, second plug-in member;

[0115] 100, kit; 1001, reagent carrying part; 1011, pre-set reagent cavity; 1012, sample inlet cavity; 1013, empty cavity; 1014, sealing film;

[0116] 300, nucleic acid amplification detection device.

[0117] Figure 28 In the present application,

[0118] 200, excitation light path; 201, fluorescent signal light path; 203, dichroic mirror; 204, filter; 205, light source; 206, detection circuit. DETAILED DESCRIPTION

[0119] The technical solutions of the present application will be further described below in conjunction with the accompanying drawings and through specific embodiments. It can be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application. In addition, it should be noted that, for the convenience of description, only the parts related to the present application are shown in the drawings, but not all.

[0120] In the present application, some orientation words are defined, and the orientation words such as "upper", "lower", "left", "right", "inner", "outer" are used for the convenience of understanding, and thus do not constitute a limitation on the protection scope of the present application.

[0121] In the present application, unless otherwise explicitly specified and limited, the "upper" or "lower" of the first feature to the second feature can include that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, the "upper", "above" and "on" of the first feature to the second feature includes that the first feature is directly above and obliquely above the second feature, or only means that the horizontal height of the first feature is higher than that of the second feature. The "under", "below" and "under" of the first feature to the second feature includes that the first feature is directly below and obliquely below the second feature, or only means that the horizontal height of the first feature is less than that of the second feature.

[0122] In the description of the present application, unless specifically defined and limited otherwise, the terms "connected", "connected", "fixed" should be understood broadly, for example, it can be fixed connection, or detachable connection, or integral; can be mechanical connection, or electrical connection; can be directly connected, or indirectly connected through intermediate media, can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0123] The technical solutions of the present application will be further described below in conjunction with the drawings and through specific embodiments. It can be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application. In addition, it should be noted that, in order to facilitate the description, only the parts related to the present application are shown in the drawings, not all.

[0124] Some orientation words are defined in the present application, and unless otherwise stated, the orientation words used such as "up", "down", "left", "right", "in", "out" are adopted for easy understanding, and therefore do not constitute a limitation on the scope of protection of the present application.

[0125] In the present application, unless otherwise specifically defined and limited, the "upper" or "lower" of the first feature to the second feature can include the direct contact of the first and second features, or can include the contact of the first and second features through another feature between them. Moreover, the "upper", "upper" and "upper" of the first feature to the second feature include the vertical and oblique above of the first feature to the second feature, or only indicate that the horizontal height of the first feature is higher than that of the second feature. The "below", "below" and "below" of the first feature to the second feature include the vertical and oblique below of the first feature to the second feature, or only indicate that the horizontal height of the first feature is less than that of the second feature.

[0126] In the description of the present application, unless specifically defined and limited otherwise, the terms "connected", "connected", "fixed" should be understood broadly, for example, it can be fixed connection, or detachable connection, or integral; can be mechanical connection, or electrical connection; can be directly connected, or indirectly connected through intermediate media, can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0127] As shown in Figure 1a and Figure 1b , the PCR automation all-in-one machine provided by the embodiment comprises a nucleic acid amplification detection device 300, the nucleic acid amplification detection device 300 comprises a nucleic acid amplification module 10 and a nucleic acid detection module 20, the nucleic acid amplification module 10 is used for amplifying a reaction sample, and the nucleic acid detection module 20 is used for detecting the reaction sample. The PCR automation all-in-one machine can also comprise a rack 40, and the nucleic acid amplification module 10 and the nucleic acid detection module 20 are both arranged in the rack 40.

[0128] The kit 100 carrying the reaction sample is placed into the nucleic acid amplification module 10 for amplification, and after amplification, detection is performed in the nucleic acid detection module 20, and the detection process is simple.

[0129] As shown in Figure 1a and Figure 1b , for example, the nucleic acid amplification detection device 300 can be two, and the nucleic acid amplification module 10 and the nucleic acid detection module 20 are arranged one by one and equal in number. Of course, the number of nucleic acid amplification detection devices 300 is not limited to two, and can also be one or more. After the nucleic acid amplification module 10 realizes amplification of the reaction sample, the carrier 4 is continuously clamped to enable the nucleic acid detection module 20 to detect the reaction sample.

[0130] In other optional embodiments, as shown in Figure 3 , the nucleic acid amplification module 10 and the nucleic acid detection module 20 are arranged separately, and after the nucleic acid amplification module 10 realizes amplification of the reaction sample, the carrier 4 of the nucleic acid amplification module 10 needs to be transferred to the nucleic acid detection module 20 to enable the nucleic acid detection module 20 to detect the reaction sample. The number of nucleic acid amplification modules 10 or nucleic acid detection modules 20 can be unequal, and the number can be one or more. Preferably, the number of nucleic acid amplification modules 10 and nucleic acid detection modules 20 can be proportionally arranged according to the time required for completing nucleic acid amplification and nucleic acid detection of the reaction sample in a single carrier 4, and the number of nucleic acid amplification modules 10 and nucleic acid detection modules 20 are matched, and the number ratio of the nucleic acid amplification module 10 to the nucleic acid detection module 20 can be no less than 1, so as to avoid insufficient utilization of the nucleic acid amplification module 10 and the nucleic acid detection module 20. If the nucleic acid amplification module 10 takes 8 minutes to complete one amplification, and the nucleic acid detection module 20 takes 2 minutes to complete one detection, then the number of nucleic acid amplification modules 10 can be 3-4 times that of the nucleic acid detection module 20.

[0131] Optionally, the PCR automation all-in-one machine can also comprise a machine shell (not shown in the figure), and the nucleic acid amplification module 10 and the nucleic acid detection module 20 are both arranged in the machine shell, so as to avoid the escape of aerosols and the like, and thus the PCR automation all-in-one machine does not need to be placed in a special room to cause pollution.

[0132] As shown in Figure 2aAs shown, the present disclosure also provides a kit 100 used with the PCR automation machine. The kit 100 is consumable, i.e. disposable. The kit 100 includes a reagent carrying part 1001 and a carrier 4. The reagent carrying part 1001 is used to carry reagents, including but not limited to sample processing liquid, PCR reaction buffer and enzyme system, etc. The carrier 4 is used to carry the reaction sample. It can be understood that the reaction sample is formed by mixing the collected sample such as throat swab or nasal swab and the reagents in the reagent carrying part 1001. After the sample is collected, the reagents in the reagent carrying part 1001 can be used to configure the reaction sample, which improves the detection efficiency. At the same time, the reagents in the reagent carrying part 1001 can be placed according to the actual amount required, and can be operated without professional test personnel, which has universality. In addition, the PCR equipment does not need to be specially provided with a container for carrying reagents and configuring reagents, and reagents do not need to be repeatedly supplemented into the PCR automation machine, thereby simplifying the structure and operation of the PCR automation machine.

[0133] The reagent carrying part 1001 can include at least one pre-set reagent cavity 1011 in which reagents are placed. The reagent carrying part 1001 can also include at least one sample inlet cavity 1012 and / or at least one empty cavity 1013. The sample inlet cavity 1012 can place throat swabs or liquid samples, and the empty cavity 1013 can be used to mix reagents. The sample inlet cavity 1012, the pre-set reagent cavity 1011 and the empty cavity 1013 can be co-arranged in five, of course, more than five or less than five according to the needs. In an optional embodiment, the sample inlet cavity 1012 is used to contain sample processing liquid, and the sample such as throat swab or nasal swab collected by the user can be placed in the sample inlet cavity 1012. The pre-set reagent cavity 1011 is provided with two and is used to contain PCR reaction buffer and enzyme system respectively.

[0134] At least one sample inlet cavity 1012, at least one pre-set reagent cavity 1011 and at least one empty cavity 1013 are provided with openings for reagents or samples to enter or exit the cavity. The opening is provided with a sealing film 1014. Before the sample is placed into the kit 100, the sealing film 1014 is opened, and before that, the cavity is isolated from the outside world, which can ensure the cleanliness of the cavity.

[0135] As shown, Figures 2a-2c The carrier 4 includes oppositely arranged first and second walls 43 and 44, and a side wall 42 arranged between the first and second walls 43 and 44, the first and second walls 43 and 44 and the side wall 42 form an amplification cavity 41, the carrier 4 is a flat structure, at least part of the side wall 42 is light transmissive, especially, the fluorescence can pass through the light transmissive side wall 42 of the amplification cavity 41, so that the nucleic acid detection module 20 can detect through the side wall 42. Preferably, the amplification cavity 41 is also a flat structure.

[0136] It can be understood that the flat structure can mean that the thickness direction of the carrier 4 or the amplification cavity 41 (that is, the direction in which the first wall 43 and the second wall 44 are arranged) has a size much smaller than the size of the direction perpendicular to the thickness direction. As an example, the ratio of the size perpendicular to the thickness direction to the size of the thickness direction is greater than 5:1, such as 50:1~100:1, and as an example, the size ratio is 90:1. As an example, the amplification cavity 41 is a cuboid, and the length and thickness of the cuboid can be greater than 5:1, such as 90:1. As an example, the thickness of the amplification cavity 41 can be 0.3-1.0mm, and the width and length of the amplification cavity 41 are about 10mm and 20mm respectively. As an example, the amplification cavity 41 can also be a cylindrical structure, and the diameter and thickness ratio is greater than 5:1, such as the thickness is 0.3-1.0mm, and the diameter is 5-20mm. Of course, the cross section of the amplification cavity 41 can be polygonal or elliptical, etc.

[0137] Optionally, the first wall 43 and the second wall 44 of the carrier 4 are made of a heat-conducting material, such as an aluminum film, or an aluminum film and a separation film, and optionally, the separation film is a polypropylene film (i.e. pp film). The separation film is in direct contact with the reaction sample, which can prevent the aluminum film from affecting the reaction sample, thereby facilitating rapid heat conduction between the reaction sample in the carrier 4 and the temperature adjusting mechanism.

[0138] As shown in Figure 2c As a preferred embodiment, the carrier 4 can further include an extrusion cavity 46, the extrusion cavity 46 is in communication with the amplification cavity 41, and the extrusion cavity 46 deforms under an external force F to extrude the amplification cavity 41, thereby enabling the extrusion cavity 46 to control the pressure in the amplification cavity 41. As in PCR amplification, the extrusion cavity 46 is extruded, and the gas and / or liquid (the liquid can be a reaction sample) in the extrusion cavity 46 pressurizes the reaction sample in the amplification cavity 41, and then the reaction sample extrudes the corresponding cavity wall of the amplification cavity 41, so that the corresponding cavity wall of the amplification cavity 41 expands outward, so that the outside of the corresponding cavity wall of the amplification cavity 41 is in full contact with the nucleic acid amplification module 10. At the same time, the reaction sample is in contact with the inside of the cavity wall of the amplification cavity 41 under the pressure of the extrusion cavity 46, and the outside of the cavity wall of the amplification cavity 41 is in full contact with the nucleic acid amplification module 10, thereby greatly improving the amplification efficiency.

[0139] As shown in Figure 1a and Figure 1b Optionally, the PCR automatic integrated machine can further include a preparation module 30, and the preparation module 30 is arranged in the rack 40. The preparation module 30 is used to prepare the reaction sample by mixing the sample and the reagent. Specifically, the preparation module 30 mixes the reagent in the reagent carrying part 1001 with the sample to form a reaction sample, and injects the reaction sample into the carrier 4. As an example, the preparation module 30 is arranged in the cabinet.

[0140] AsFigure 1a As shown, the preparation module 30 comprises a carrying sub-module 31 and a pipetting sub-module 32 arranged on the upper side of the carrying sub-module 31. The carrying sub-module 31 and the pipetting sub-module 32 are capable of relative movement, so that the pipetting sub-module 32 transfers the solution on the carrying sub-module 31, mixes the sample and the reagent to form a reaction sample, and the carrying sub-module 31 and the pipetting sub-module 32 are capable of relative movement, and the reaction sample in the reagent carrying part 1001 can be injected into the carrier 4. The carrying sub-module 31 and the pipetting sub-module 32 cooperate to complete the preparation of the reaction sample and place the reaction sample into the carrier 4, so as to prepare for nucleic acid amplification.

[0141] As shown in the specific embodiment, the carrying sub-module 31 is used to carry the reagent box 100, and the pipetting sub-module 32 is used to transfer the solution between the cavities of the reagent box 100. That is, the pipetting sub-module 32 can suck the sample, the reagent and the reaction sample in the reagent box 100, and transfer the sample, the reagent and the reaction sample. Figure 1b

[0142] Optionally, the pipetting sub-module 32 comprises a pipetting gun 321, which can suck the reagent and discharge the reagent to the reagent box 100. At least one of the pipetting gun 321 and the carrying sub-module 31 is capable of moving in the horizontal plane, and at least one of the pipetting gun 321 and the carrying sub-module 31 is capable of moving in the vertical direction.

[0143] For example, the carrying sub-module 31 is located below the pipetting sub-module 32. The carrier 4, the sample inlet cavity 1012, the pre-reagent cavity 1011 and the empty cavity 1013 are arranged along the first direction (such as the X direction shown in FIG. 2), and the pipetting gun 321 of the pipetting sub-module 32 reciprocates relative to the rack 40 along the first direction and the vertical direction (such as the Z direction shown in FIG. 1 and FIG. 2) to realize liquid preparation. The first direction is perpendicular to the vertical direction, and the first direction is the horizontal direction. Figure 1a

[0144] The pipetting sub-module 32 further comprises a vertical driving member 322 and a horizontal driving assembly. The vertical driving member 322 is connected with the pipetting gun 321 to drive the pipetting gun 321 to reciprocate in the vertical direction, and the horizontal driving assembly is connected with the vertical driving member 322 to drive the vertical driving member 322 to reciprocate in the first direction, thereby driving the pipetting gun 321 to reciprocate in the first direction. The horizontal driving assembly and the vertical driving member 322 can be connected through a pipetting connecting member 324. Optionally, the vertical driving member 322 can be a pneumatic cylinder or an electric cylinder, which can drive the pipetting gun 321 to reciprocate in the vertical direction.

[0145] ​​For example, the horizontal drive assembly includes a horizontal drive member 323, a pipetting gear 325, and a rack 326. The horizontal drive member 323 can be a servo motor, etc. The pipetting gear 325 is connected to the output end of the horizontal drive member 323 and meshes with the rack 326 for transmission. The rack 326 is connected to the frame 40 and extends along the first direction. The horizontal drive member 323, such as the servo motor, rotates forward and reverse, thereby driving the pipetting gear 325 to rotate forward and reverse. The pipetting gear 325 meshes with the rack 326 for transmission, causing the horizontal drive member 323 to reciprocate along the first direction, thereby driving the vertical drive member 322 and the pipette 321 to reciprocate along the first direction.

[0146] like Figure 1b As shown, when pipette 321 needs to aspirate reagents from reagent kit 100, vertical drive 322 drives pipette 321 to move downwards and insert into the reagent to aspirate the reagent. After aspiration, vertical drive 322 drives pipette 321 to move upwards, positioning it above reagent kit 100. Horizontal drive component drives reagent kit 100 to translate, so that pipette 321 is vertically aligned with the chamber requiring liquid injection. Then, vertical drive 322 drives pipette 321 to move downwards, injecting the reagent into another chamber of reagent kit 100.

[0147] like Figure 4 The diagram illustrates the process of preparing reaction samples and injecting them into vector 4 using a fully automated PCR machine:

[0148] Step 1: Manually or automatically tear open the sealing film 1014 outside the PCR automated integrated machine;

[0149] Step 2: The collected sample is placed into the injection chamber 1012 by manual or automated equipment outside the PCR automated integrated machine;

[0150] Step 3: Place the reagent kit 100 into the carrier submodule 31. The vertical drive component 322 and the horizontal drive component drive the pipette 321 to move. The pipette 321 transfers the PCR reaction buffer in one of the pre-placed reagent chambers 1011 into the empty chamber 1013.

[0151] Step 4: The vertical drive component 322 and the horizontal drive component drive the pipette 321 to move, and the pipette 321 transfers the enzyme system in another pre-placed reagent chamber 1011 to the cavity 1013.

[0152] Step 5: The vertical drive component 322 and the horizontal drive assembly drive the pipette 321 to move, and the pipette 321 transfers the sample processing liquid in the injection chamber 1012 to the cavity 1013.

[0153] Step 6: Mix the reaction sample in cavity 1013 using pipette 321;

[0154] Step 7, the vertical driving member 322 and the horizontal driving assembly drive the pipette 321 to move, and the pipette 321 transfers the reaction sample in the cavity 1013 to the carrier 4;

[0155] Step 8, the liquid injection is completed.

[0156] As shown in Figure 1a and Figure 1b Optionally, the PCR automation all-in-one machine further comprises a transfer module 60 arranged on the rack 40, and the transfer module 60 is used to transfer the reagent box 100 or the carrier 4 between the carrying sub-module 31 and the nucleic acid amplification module 10, or as shown in Figure 3 , the transfer module 60 is used to transfer the reagent box 100 or the carrier 4 between the carrying sub-module 31, the nucleic acid amplification module 10 and the nucleic acid detection module 20. As an example, the transfer module 60 is arranged above the nucleic acid amplification module 10 and the carrying sub-module 31. The configuration, transfer, amplification and detection of the reaction sample can be automatically completed, so that professional testers are not required, and the operation is simple, and the requirement of PCR detection professionals is low. As an example, the transfer module 60 is arranged in the cabinet, and preferably, each module is arranged in the cabinet, and an air purification module can be arranged in the cabinet, so as to avoid pollution caused by the escape of aerosol and the like.

[0157] As shown in Figure 1a and Figure 1b , the transfer module 60 provided by the present disclosure comprises a horizontal transfer driving assembly, a vertical transfer driving member 66 and a pick-and-place member 61, the pick-and-place member 61 is used to grab and place the carrier 4, the reagent carrying part 1001 or the reagent box 100, the vertical transfer driving member 66 is connected with the pick-and-place member 61 to drive the pick-and-place member 61 to reciprocate in the vertical direction, the horizontal transfer driving assembly is connected with the vertical transfer driving member 66 to drive the vertical transfer driving member 66 to reciprocate in the first direction, thereby driving the pick-and-place member 61 to reciprocate in the first direction. Wherein, the horizontal transfer driving assembly and the vertical transfer driving member 66 can be connected through a transfer connecting member 69. Optionally, the vertical transfer driving member 66 can be a pneumatic cylinder or an electric cylinder, which can drive the pick-and-place member 61 to reciprocate in the vertical direction.

[0158] The pick-and-place member 61 can transfer the carrier 4, the reagent carrying part 1001 or the reagent box 100 through vacuum adsorption or clamping. As an example, when the pick-and-place member 61 transfers the carrier 4, the reagent carrying part 1001 or the reagent box 100 in the clamping mode, the pick-and-place member 61 can be as shown in Figure 1a and Figure 1bThe air-fingered device, etc. shown, when the taking and placing member 61 adopts the vacuum suction mode to transfer the carrier 4, the reagent carrying part 1001 or the reagent box 100, the taking and placing member 61 can include a suction cup connected to a vacuum pump, etc. to generate a vacuum environment between the suction cup and the suction reagent box 100 or the carrier 4, and then to suck the reagent carrying part 1001, the reagent box 100 or the carrier 4.

[0159] For example, the horizontal transfer driving assembly includes a horizontal transfer driving member 67, which can be a servo motor, etc., a transfer gear 68 connected to the output end of the horizontal transfer driving member 67, and a transfer rack. The transfer gear 68 is in meshing transmission with the transfer rack. Optionally, the transfer rack is the above-mentioned rack 326, that is, the pipetting sub-module 32 and the transfer module 60 share the same rack 326, that is, as shown in Figure 1b The horizontal driving assembly and the horizontal transfer driving assembly are connected to the rack 326 in the first direction, and the pipetting gear 325 of the horizontal driving assembly and the transfer gear 68 of the horizontal transfer driving assembly are respectively in meshing transmission with the rack 326. When the transfer module 60 picks up the carrier 4 located on the carrying sub-module 31, the pipetting sub-module 32 can be located at one end of the rack 326, which is the end of the rack 326 close to the carrying sub-module 31, that is, as shown in Figure 1b The position shown to avoid interference with the transfer module 60. The horizontal transfer driving member 67, such as a servo motor, rotates forward and reverses, thereby driving the transfer gear 68 to rotate forward and reverse. The transfer gear 68 is in meshing transmission with the rack 326, so that the horizontal transfer driving member 67 moves back and forth in the first direction, thereby driving the vertical transfer driving member 66 and the taking and placing member 61 to move back and forth in the first direction.

[0160] The working process of the transfer module 60 is described below by taking the transfer of the carrier 4 from the carrying sub-module 31 to the nucleic acid amplification module 10 as an example:

[0161] The horizontal transfer driving assembly drives the taking and placing member 61 to be located directly above the carrier 4 on the carrying sub-module 31;

[0162] The vertical transfer driving member 66 drives the taking and placing member 61 to move downward to pick up the carrier 4;

[0163] The vertical transfer driving member 66 drives the taking and placing member 61 to move upward to avoid collision between the carrier 4 and other structures during the transfer of the carrier 4;

[0164] The horizontal transfer driving assembly drives the taking and placing member 61 to move to the side where the nucleic acid amplification module 10 is located until it is directly above the nucleic acid amplification module 10;

[0165] The vertical transfer driving member 66 drives the taking and placing member 61 to move downward to insert the carrier 4 into the nucleic acid amplification module 10.

[0166] It can be understood that the carrier 4 corresponding to the transfer module 60 is vertically placed when being placed into the shell, so that the carrier 4 does not need to be rotated, and the transfer module 60 can only drive the carrier 4 to translate to be vertically inserted into the nucleic acid amplification module 10. As shown in Figure 7a the thickness direction of the carrier 4 is perpendicular to the depth direction of the cavity of the reagent carrying part 1001, so as to ensure that the carrier 4 is not rotated and can be vertically inserted into the nucleic acid detection module 20, and meanwhile, the opening of the cavity of the reagent carrying part 1001 faces upward. Of course, the carrier 4 and the reagent carrying part 1001 can also be separately provided, and at this time, the transfer module 60 only drives the carrier 4 to translate to vertically insert the carrier 4 into the nucleic acid amplification module 10.

[0167] As shown in Figure 1a and Figure 1b Optionally, the PCR automatic integrated machine further comprises a recovery module 90 arranged in the shell and used for recovering the reagent box 100. The recovery module 90 is connected to the rack 40 and located below the transfer module 60.

[0168] The transfer module 60 can transfer the reagent box 100 in the shell to the recovery module 90. Specifically, the transfer module 60 can grasp the reagent box 100, the reagent carrying part 1001 or the detection-completed carrier 4 and transfer them to the recovery module 90.

[0169] As shown in FIG. 1, the carrier sub-module 31, the nucleic acid amplification detection device 300 and the recovery module 90 are sequentially arranged along the first direction, so as to facilitate the arrangement of the transfer module 60 and improve the compactness of the structure of the PCR automatic integrated machine.

[0170] As shown in Figure 3 and Figure 5a To facilitate stable placement of the reagent box 100, the carrier sub-module 31 optionally comprises a bracket 311, and the bracket 311 is provided with a containing part for containing the reagent box 100. The containing part can limit the reagent box 100 on one hand, so as to ensure that each reagent box 100 is placed at a fixed position on the carrier sub-module 31; on the other hand, the reagent box 100 can be limited from moving relative to the bracket 311 during the entire pipetting process, so as to ensure that the pipetting gun 321 can accurately enter the cavity of the reagent box 100.

[0171] Optionally, the containing part comprises a plurality of containing grooves 3111, and the reagent carrying part 1001 of the reagent box 100 can be at least partially inserted into the containing grooves 3111. Further, the containing grooves 3111 correspond to the cavities of the reagent box 100, and each cavity is inserted into a containing groove 3111. The groove wall 3113 between adjacent two containing grooves 3111 can support the reagent box 100, so as to improve the stability of the reagent box 100.

[0172] At least one of the plurality of accommodation grooves 3111 is provided with a heating structure 3112. The heating structure 3112 can preheat the reagent in the reagent kit 100, so that the reagent is preheated to a suitable temperature, thereby reducing the time required for the reaction sample to be amplified in the nucleic acid amplification module 10. For example, the heating structure 3112 can heat the reagent to 25-80°C ± 1°C. It can be understood that the heating structure 3112 can be a heating element such as a heating wire, as long as it can be built into the accommodation groove 3111 and heat the solution. The heating structure 3112 is a prior art, and thus will not be described here. At the same time, the accommodation groove 3111 corresponds to each cavity of the reagent kit 100, and the environmental temperature of each cavity of the reagent kit 100 can also be controlled separately.

[0173] As shown in Figure 5a , the bracket 311 can support the carrier 4 and the reagent carrying part 1001. One of the accommodation grooves 3111 can accommodate the carrier 4, and the carrier 4 can be placed horizontally on the bracket 311, that is, the thickness direction of the carrier 4 is perpendicular to the vertical direction. Figure 5b As shown in , the bracket 311 can also be used to support only the reagent carrying part 1001, and not the carrier 4, and the carrier 4 is placed in the air.

[0174] Figure 6a In this embodiment, preferably, the nucleic acid amplification module 10 and / or the nucleic acid detection module 20 vertically insert the carrier 4, that is, the thickness direction of the carrier 4 is perpendicular to the vertical direction. When performing pcr amplification and / or detection, the carrier 4 is placed vertically, that is, the carrier 4 is inserted into the nucleic acid amplification module 10 and / or the nucleic acid detection module 20 in the direction indicated by the arrow in Figure 6b and Figure 6a , and the thickness direction of the carrier 4 (the X direction shown in Figure 6b and Figure 6a ) is perpendicular to the vertical direction (the Z direction shown in Figure 6b and , and the X, Y and Z directions are perpendicular to each other). In this way, when the carrier 4 is inserted into the nucleic acid amplification module 10 and / or the nucleic acid detection module 20, the bubbles can float to the top of the carrier 4, avoiding the bubbles being dispersed everywhere on the side wall 42 of the carrier 4, thereby ensuring the accuracy of the nucleic acid detection module 20 and avoiding the problem of uneven temperature of the reaction sample. In the heating process, the gas in the reaction sample is precipitated to form bubbles, and when the carrier 4 is vertically inserted, the bubbles can flow upward to the top end of the carrier 4, thereby not affecting the detection and amplification. At the same time, the convection generated by the gravity of the reaction sample and the disturbance of the reaction sample by the movement of the bubbles can further increase the consistency of the temperature of the reaction sample.

[0175] 7aUsing the PCR automation all-in-one machine provided in the embodiment, one reagent box 100 can enter the PCR automation all-in-one machine for detection, without the need to collect a large number of samples for unified detection, and the detection result can be quickly given.

[0176] As shown in Figure 1b , optionally, the PCR automation all-in-one machine further comprises a cutting module 70 (a first cutter 703 as shown in Figure 1b ), which is used to cut the reagent box 100 between the carrier 4 and the reagent carrying part 1001. The cutting module 70 can cut the reagent box 100 before the transfer module 60 transfers the carrier 4 to the nucleic acid amplification module 10. After the reagent box 100 is cut, the transfer module 60 can only transfer the carrier 4 to the nucleic acid amplification module 10 and the nucleic acid detection module 20, so that the reagent carrying part 1001 can avoid occupying space in the nucleic acid amplification module 10 and the nucleic acid detection module 20, thereby improving the compactness of the structure of the PCR automation all-in-one machine and reducing the space occupied by the equipment.

[0177] Of course, in other alternative embodiments, the carrier 4 and the reagent carrying part 1001 can be provided separately, so that the reagent box 100 does not need to be cut and the cutting module 70 does not need to be provided.

[0178] As shown in Figure 1b , the first cutting module 70 is exemplarily disclosed to include the first cutter 703, a storage groove can be formed on the carrying sub-module 31, the first cutter 703 can be placed in or taken out of the storage groove, and after the first cutter 703 is placed in the storage groove, one end thereof extends out of the storage groove. The taking and placing part 61 of the transfer module 60 (at this time, the taking and placing part 61 transfers the carrier 4 by clamping to facilitate clamping the first cutter 703) clamps one end of the first cutter 703 extending out of the storage groove to cut the reagent box 100.

[0179] Exemplarily, the process of cutting the reagent box 100 can be as follows:

[0180] The horizontal transfer driving assembly drives the taking and placing part 61 to be located directly above the first cutter 703;

[0181] The vertical transfer driving part 66 drives the taking and placing part 61 to move downward to pick up the first cutter 703;

[0182] The vertical transfer driving part 66 drives the taking and placing part 61 to move upward to take the first cutter 703 out of the storage groove;

[0183] The horizontal transfer driving assembly drives the taking and placing part 61 to move to the side where the reagent box 100 is located until it is directly above the position where the reagent box 100 needs to be cut;

[0184] The vertical transfer drive 66 drives the pick-and-place component 61 to move downward to cut the reagent kit 100;

[0185] The horizontal transfer drive assembly drives the pick-and-place component 61 to move the first cutter 703 toward the side where the storage slot is located, until it is directly above the storage slot;

[0186] The vertical transfer drive 66 drives the pick-and-place member 61 to move downwards so as to place the first cutter 703 into the storage slot.

[0187] like Figure 7a As shown, an exemplary second cutting module 70 is disclosed. The cutting module 70 includes a cutting drive component 701 and a second cutter 702. The cutting drive component 701 is used to drive the second cutter 702 to approach and cut the reagent kit 100, and to drive the second cutter 702 to avoid the reagent kit 100, so that the pipette 321 pipettes liquid, and the transfer module 60 transfers the reagent kit 100.

[0188] Specifically, the cutting drive assembly 701 includes a cutting rotation drive component and a crank-connecting rod-slider mechanism. The cutting rotation drive component can be a motor or the like, to drive one end of the crank-connecting rod-slider mechanism to rotate. The second cutter 702 is connected to the other end of the crank-connecting rod-slider mechanism to enable the second cutter 702 to move up and down.

[0189] Optionally, the cutting drive assembly 701 can be connected to the frame 40 via a drive structure (not shown in the figure). The drive structure drives the cutting drive assembly 701 to move along a first direction, so as to avoid the transfer module 60 and the pipetting submodule 32 when they move along the first direction. The drive structure can be the same as the structure of the horizontal drive assembly and the horizontal transfer drive assembly, and share the rack 326 with the horizontal drive assembly and the horizontal transfer drive assembly.

[0190] like Figure 7b and Figure 7c As shown, optionally, the PCR automation integrated machine also includes a flipping module 7 disposed on the rack. The flipping module 7 is used to flip the carrier of the reagent kit by a preset angle. When the carrier 4 is placed on the carrier submodule 31, and the thickness direction of the carrier 4 is parallel to the vertical direction, the flipping module 7 can flip the carrier 4 by 90°, so that the thickness direction of the carrier 4 is perpendicular to the vertical direction. It can be understood that the reagent carrier part 1001 and the carrier 4 of the reagent kit 100 can be separate structures, and the flipping module 7 only flips the carrier 4 by 90°, or the reagent carrier part 1001 and the carrier 4 of the reagent kit 100 can be a single structure, and the reagent carrier part 1001 and the carrier 4 can be separated by the cutting module 70, so that the flipping module 7 only flips the carrier 4 by 90°.

[0191] like Figure 7cAs shown, the turnover module 7 can be arranged on the bearing sub-module 31, and the turnover module 7 can be located at one side of the first cutter 703. The turnover module 7 can include a turnover driving member 71 and a first plug-in member 72. The turnover driving member 71 can be a stepper motor or the like. The output end of the turnover driving member 71 is connected with the first plug-in member 72, so as to drive the first plug-in member 72 to turn over. The first plug-in member 72 can include a first plug-in slot, and the carrier 4 can be plugged into the plug-in slot. The turnover driving member 71 drives the first plug-in member 72 to turn over by 90°, and the carrier 4 in the first plug-in slot turns over by 90°. When the first plug-in member 72 is not turned over, the first plug-in slot can extend in the horizontal direction, so as to facilitate plugging of the carrier 4, and when the carrier 4 is turned over, the carrier 4 is prevented from being separated from the first plug-in slot.

[0192] Optionally, the turnover module 7 can further include a second plug-in member 73. The second plug-in member 73 can include a second plug-in slot, and the reagent bearing part 1001 can be arranged in the second plug-in slot. The second plug-in slot can extend in the horizontal direction, so as to facilitate plugging of the reagent bearing part 1001.

[0193] The turnover module 7 provided in the embodiment has simple structure and convenient operation, and can improve the compactness of the PCR automatic integrated machine.

[0194] As shown in Figs. 1 and 2, Figure 1b and Figure 8a in other optional embodiments, the turnover module 7 can not be arranged, but the PCR automatic integrated machine further includes a bending module 80 arranged on the rack 40. The bending module 80 is used to bend one end of the reagent box 100 by a preset angle. Specifically, the bending module 80 bends the carrier 4 of the reagent box 100 by a preset angle, such as 90°. Figure 8a As shown, the bending module 80 bends the horizontally placed carrier 4 to be placed vertically. In order to facilitate bending of the carrier 4, optionally, a bending gap 45 is arranged at the connection position between the carrier 4 and the reagent bearing part 1001.

[0195] Optionally, the reagent box 100 is arranged on the bearing sub-module 31, and one end of the reagent box 100 to be bent is suspended. In the embodiment, the end to be bent is the carrier 4. The reagent bearing part 1001 can be placed on the bracket 311 as shown in Fig. 3, and the bracket 311 can suspend the carrier 4. Figure 5b

[0196] As shown in Figs. 1 and 2, Figure 1b and Figure 8a ​, as shown, the first bending module 80 includes a first roller 805 and a handle 806 connected to each other, a storage groove can be formed on the carrier sub-module 31, and the first roller 805 and the handle 806 can be placed in or taken out of the storage groove. After the bending module 80 is placed in the storage groove, one end of the handle 806 extends out of the storage groove. The transfer module 60 drives the taking and placing part 61 to take and place the end of the handle 806 extending out of the storage groove, and the reagent box 100 is bent.

[0197] , as shown, the first bending module 80 includes a first roller 805 and a handle 806 connected to each other, a storage groove can be formed on the carrier sub-module 31, and the first roller 805 and the handle 806 can be placed in or taken out of the storage groove. After the bending module 80 is placed in the storage groove, one end of the handle 806 extends out of the storage groove. The transfer module 60 drives the taking and placing part 61 to take and place the end of the handle 806 extending out of the storage groove, and the reagent box 100 is bent.

[0198] The horizontal transfer driving assembly drives the taking and placing part 61 to be located directly above the handle 806;

[0199] The vertical transfer driving part 66 drives the taking and placing part 61 to move downward to pick up the bending module 80; (Since the vertical transfer driving part 66 can be a pneumatic cylinder or an electric cylinder, etc., when the vertical transfer driving part 66 drives the taking and placing part 61 to be located at a higher position, the lower end of the vertical transfer driving part 66 is also at a higher position. Therefore, when the horizontal transfer driving assembly drives the vertical transfer driving part 66 to move in the first direction, it will not interfere with other modules.)

[0200] The vertical transfer driving part 66 drives the taking and placing part 61 to move upward to take out the bending module 80 from the storage groove;

[0201] The horizontal transfer driving assembly drives the taking and placing part 61 to move to the side of the reagent box 100, until the first roller 805 moves to the upper side of the carrier 4;

[0202] The vertical transfer driving part 66 drives the taking and placing part 61 to move downward until the first roller 805 abuts to the carrier 4;

[0203] Bend the reagent box 100: the vertical transfer driving part 66 drives the taking and placing part 61 to continue to move downward (since the vertical transfer driving part 66 can be a pneumatic cylinder or an electric cylinder, etc., it can drive the taking and placing part 61 to move downward, and in turn drive the bending module 80 to move downward), and the horizontal transfer driving assembly drives the taking and placing part 61 to the reagent carrier 1001, so that the second roller 804 moves in a circular arc track to always abut to the surface of the carrier 4, and in turn pushes the carrier 4 to bend. The second roller 804 can reduce the friction between it and the surface of the carrier 4, and in turn protect the carrier 4 from being scratched. Figure 8a , as shown, the carrier 4 is in the initial state, the intermediate state and the final state. During the bending process, the pipette 321 can abut to the reagent carrier 1001 to prevent the reagent carrier 1001 from being raised upward;

[0204] Returning the bending module 80 to the storage slot: The horizontal transfer drive component drives the pick-and-place component 61 to move the bending module 80 toward the side where the storage slot is located until it is directly above the storage slot; the vertical transfer drive component 66 drives the pick-and-place component 61 to move downward to place the bending module 80 into the storage slot.

[0205] The pick-and-place component 61 of the transfer module 60 returns and picks up the carrier 4 to transfer the carrier 4 to the nucleic acid amplification module 10.

[0206] like Figure 8b As shown, optionally, this disclosure also provides a structural schematic diagram of a second type of bending module 80, in which the bending module 80 is movably connected to the frame 40 along the first direction.

[0207] The bending module 80 includes a dual-axis module. The lower end of the dual-axis module can move along a first direction and a vertical direction to always press against the upper surface of the carrier 4 and bend the carrier 4 at a preset angle. Figure 8b The diagram shows the positions of carrier 4 in the initial, intermediate, and final states.

[0208] Optionally, the dual-axis module includes a horizontal drive component 802, a vertical drive component 803, and a second roller 804. The vertical drive component 803 is connected to the output end of the horizontal drive component 802, and the second roller 804 is connected to the output end of the vertical drive component 803. The horizontal drive component 802 is used to drive the vertical drive component 803 and the second roller 804 to move along a first direction, that is, to realize the movement of the bending module 80 relative to the frame 40 along the first direction. The horizontal drive component 802 can have the same structure as the horizontal drive assembly and the horizontal transfer drive assembly, or it can share the rack 326 with the horizontal drive assembly and the horizontal transfer drive assembly. In this case, the horizontal drive component 802 is connected to the frame 40 through the rack 326, and the horizontal drive component 802, the horizontal drive assembly, and the horizontal transfer drive assembly are spaced apart on the frame 40 along the first direction. The vertical drive component 803 is used to drive the second roller 804 to move in the vertical direction, and the structure of the vertical drive component 803 can be the same as the structure of the vertical transfer drive component 66. The second roller 804 can press against the upper surface of the carrier 4.

[0209] During the bending process of the carrier 4, the lateral drive member 802 and the vertical drive member 803 cooperate to move the second roller 804 along an arc trajectory, ensuring it always presses against the surface of the carrier 4, thereby pushing the carrier 4 to bend. The second roller 804 can reduce the friction between itself and the surface of the carrier 4, thus protecting the carrier 4 from scratches. On the other hand, as... Figure 8b and Figure 8cAs shown, the horizontal driving member 802 drives the vertical driving member 803 and the second roller 804 to move in the first direction, so as to avoid the transfer module 60 and the pipetting sub-module 32 when the transfer module 60 and the pipetting sub-module 32 move in the first direction. For example, Figure 8c As shown, the right end of the rack 326 extends out of the right end of the carrying sub-module 31, and the bending module 80 and the pipetting sub-module 32 can move to the right end of the rack 326 to avoid the transfer module 60, thereby avoiding interference with the transfer module 60.

[0210] Optionally, the bending module 80 can further include a pressing column 801 capable of pressing against the upper surface of the reagent carrying portion 1001, and the pressing column 801 can be connected with a cylinder or other pressing column lifting driving member 807, which can drive the pressing column 801 to reciprocate in the vertical direction to press against the carrier 4, so as to prevent the reagent carrying portion 1001 from being raised upward during the process of bending the carrier 4. The pressing column lifting driving member 807 can be further connected with a bending driving structure 808, which drives the pressing column lifting driving member 807 and the pressing column 801 to move in the first direction to avoid the transfer module 60 and the pipetting sub-module 32 when the transfer module 60 and the pipetting sub-module 32 move in the first direction. The bending driving structure 808 can have the same structure as the horizontal driving assembly and the horizontal transfer driving assembly, and can also share the rack 326 with the horizontal driving assembly and the horizontal transfer driving assembly.

[0211] Of course, the bending module 80 can also not include the pressing column 801, the pressing column lifting driving member 807 and the bending driving structure 808, but can be the same as the first bending module 80, that is, by making the pipetting gun 321 abut against the reagent carrying portion 1001 to prevent the reagent carrying portion 1001 from being raised upward.

[0212] In other optional embodiments, the bending module 80 bends the carrier 4 of the reagent box 100 by a preset angle, such as 90°, and breaks the reagent box 100, such as breaking the reagent box 100 between the carrier 4 and the reagent carrying portion 1001. For example, at least the connection between the carrier 4 and the reagent carrying portion 1001 is made of a brittle material, such as acrylic (i.e., PMMA), polystyrene (i.e., PS) or the like, so as to facilitate the reagent box 100 to be broken after the reagent box 100 is bent. The connection between the carrier 4 and the reagent carrying portion 1001 can be provided with a stamp hole or other breaking opening which is easy to break the reagent box 100. In addition, the carrying sub-module 31 can be provided with a guide groove which is adapted to the contour of the carrier 4, so as to be able to keep the carrier 4 in the vertical state when the carrier 4 is bent to the vertical state.

[0213] For example, Figure 1b and Figure 9 For the convenience of showing the structure of the nucleic acid amplification module 10, Figure 9As shown in the figure (the placement direction of the nucleic acid amplification module 10 is not the placement direction when it is in use), in this embodiment, the nucleic acid amplification module 10 includes at least one set of temperature regulation mechanisms. The temperature regulation mechanisms can change the temperature of the reaction sample in the carrier 4 so that the temperature of the reaction sample can be repeatedly thermally cycled in three stages: high temperature denaturation, low temperature annealing and suitable temperature extension.

[0214] For example, the nucleic acid amplification module 10 includes two sets of temperature regulation mechanisms, such as... Figure 9 The two sets of temperature regulating mechanisms can be moved closer or further apart to allow the carrier 4 to be sandwiched between them. Specifically, the two sets of temperature regulating mechanisms are a first temperature regulating mechanism 52 and a second temperature regulating mechanism 56. The positions of the first temperature regulating mechanism 52 and / or the second temperature regulating mechanism 56 are adjustable to move closer or further away from the second temperature regulating mechanism 56, thus sandwiching the carrier 4 between the first temperature regulating mechanism 52 and the second temperature regulating mechanism 56. For example, the first temperature regulating mechanism 52 abuts against one of the first wall 43 and the second wall 44 of the carrier 4, and the second temperature regulating mechanism 56 abuts against the other of the first wall 43 and the second wall 44 of the carrier 4, thereby clamping the carrier 4, while the side wall 42 of the carrier 4 is exposed for detection by the nucleic acid detection module 20.

[0215] like Figure 1b As shown, the first temperature regulating mechanism 52 and the second temperature regulating mechanism 56 are arranged along the first direction, and the nucleic acid detection module 20 can be arranged in the second direction of the nucleic acid amplification module 10 (e.g., ...). Figure 1b The nucleic acid detection module 20 can be positioned on one or both sides of the carrier 4 (as shown in the Y direction) so that when the nucleic acid amplification module 10 clamps the carrier 4, the nucleic acid detection module 20 can detect the reaction sample inside the carrier 4 through the sidewall 42 of the carrier 4. The first direction, the second direction, and the vertical direction are mutually perpendicular. Alternatively, in other optional embodiments, the first temperature regulating mechanism 52 and the second temperature regulating mechanism 56 are arranged along the second direction, and the nucleic acid detection module 20 can be positioned on one or both sides of the nucleic acid amplification module 10 in the first direction.

[0216] Specifically, such as Figure 10 As shown (for ease of demonstrating the structure of nucleic acid amplification module 10), Figure 10 (The placement orientation of the nucleic acid amplification module 10 is not the orientation during its use.) When the first temperature regulating mechanism 52 moves away from the second temperature regulating mechanism 56, the carrier 4 can be removed from between the first temperature regulating mechanism 52 and the second temperature regulating mechanism 56, or the carrier 4 can be placed between the first temperature regulating mechanism 52 and the second temperature regulating mechanism 56.

[0217] like Figure 11 and Figure 9As shown, when the first temperature regulating mechanism 52 approaches the second temperature regulating mechanism 56, the first temperature regulating mechanism 52 and the second temperature regulating mechanism 56 can clamp the carrier 4, thereby heating or cooling the carrier 4. At this time, the fluorescence detection optical path system 201 performs fluorescence detection through the side wall 42 of the carrier 4. The fiber optic seat 2012 is set on one side of the carrier 4 to fix the fiber optic cable, so that the fiber optic cable performs fluorescence detection through the side wall 42 of the carrier 4.

[0218] like Figure 11 The diagram shows a top view of the nucleic acid detection module 20 and the nucleic acid amplification module 10 in normal use. This arrangement allows the carrier 4 to be inserted between the first temperature regulating mechanism 52 and the second temperature regulating mechanism 56, with the carrier 4 placed vertically. At this time, the carrier submodule 31 can be positioned between the nucleic acid detection module 20 and the nucleic acid amplification module 10 as shown. Figure 11 The side shown (in) Figure 11 The carrier submodule 31 is disposed on the upper side of the nucleic acid detection module 20 and the nucleic acid amplification module 10. In other optional embodiments, the carrier submodule 31 may be disposed on the lower side of the nucleic acid detection module 20 and the nucleic acid amplification module 10. It should be understood that the upper and lower sides refer to the directions shown in the figure, not the actual directions in use.

[0219] like Figure 9 As shown, the nucleic acid amplification module 10 includes a driving mechanism 53, which drives a first temperature regulating mechanism 52 and / or a second temperature regulating mechanism 56 to move closer to or further away from each other. The following description uses the example of the driving mechanism 53 driving the first temperature regulating mechanism 52 to move closer to or further away from the second temperature regulating mechanism 56 as an example.

[0220] like Figure 12 and Figure 13 As shown, optionally, the nucleic acid amplification module 10 may include a support base 55, and the driving mechanism 53 and the second temperature adjustment mechanism 56 may be disposed on the support base 55.

[0221] like Figure 12 As shown, to ensure stable movement of the first temperature regulating mechanism 52, the nucleic acid amplification module 10 may optionally include a guide mechanism 54 for guiding the temperature regulating mechanism. The guide mechanism 54 may be mounted on a support base 55, which may be connected to the frame 40.

[0222] Specifically, the guide mechanism 54 includes a guide rail 541 and a slider 542. The guide rail 541 is mounted on the support base 55, and the slider 542 is slidably connected to the guide rail 541. The first temperature regulating mechanism 52 is connected to the slider 542. The slider 542 slides along the guide rail 541, thereby driving the first temperature regulating mechanism 52 to slide.

[0223] As shown in Figure 12 and Figure 13 , the driving mechanism 53 comprises a driving assembly and a mounting frame 532 connected to an output end of the driving assembly, specifically, the first temperature adjusting mechanism 52 is connected to the sliding block 542 through the mounting frame 532.

[0224] As shown in Figure 13 , and in combination with Figure 12 and Figure 14 , the driving assembly can comprise a driving member 531 such as a servo motor and a transmission assembly, the transmission assembly can comprise a driving gear 535 (as shown in Figure 18 ), a belt (not shown in the figure), a driven gear 536, a screw rod 533 and a nut 534, wherein the driving member 531 can be fixed on the support base 55 (as shown in Figure 12 ), and the screw rod 533 is rotatably connected to the support base 55 through a bearing 537. The output end of the driving member 531 can be connected to the driving gear 535, one end of the screw rod 533 is connected to the driven gear 536, and the driven gear 536 and the driving gear 535 are drivingly connected through the belt. The nut 534 is threadedly connected to the screw rod 533, and the mounting frame 532 is connected to the nut 534.

[0225] The driving member 531 drives the driving gear 535 to rotate, the driving gear 535 drives the belt to rotate, and the belt drives the driven gear 536 to rotate. Since the driven gear 536 is connected to the screw rod 533, the screw rod 533 can be driven to rotate. The nut 534 is threadedly connected to the screw rod 533, so that the nut 534 moves along the extending direction of the screw rod 533, and drives the mounting frame 532 to move, and the mounting frame 532 drives the first temperature adjusting mechanism 52 to move. Alternatively, the screw rod 533 extends in a first direction, and the extending direction of the screw rod 533 is consistent with the direction in which the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 are arranged in sequence, so that when the nut 534 moves along the screw rod 533, the first temperature adjusting mechanism 52 is driven to move close to or away from the second temperature adjusting mechanism 56.

[0226] Of course, in other alternative embodiments, the driving mechanism 53 can also drive the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 to move towards or away from each other at the same time. At this time, the screw rod 533 can be a bidirectional screw rod 533 with positive and reverse threads, and the two groups of temperature adjusting mechanisms are connected with the mounting frame 532, and the mounting frame 532 is connected with the nut 534. The nut 534 connected with the first temperature adjusting mechanism 52 is connected to the positive thread of the screw rod 533, and the nut 534 connected with the second temperature adjusting mechanism 56 is connected to the reverse thread of the screw rod 533. When the driving part 531 drives the screw rod 533 to rotate, the nut 534 on the positive thread and the nut 534 on the reverse thread move towards each other, so that the driving mechanism 53 drives the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 to move towards or away from each other at the same time.

[0227] As shown in Figure 13 and Figure 14 , the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 each include a cooling assembly 521 for cooling the carrier 4. Optionally, the cooling assemblies 521 of the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 are symmetrically arranged to cool both sides of the carrier 4 at the same time.

[0228] Optionally, the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 each further include a heater 522 arranged on the side of the cooling assembly 521 of the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 close to each other. The heater 522 is used to heat the carrier 4. In this embodiment, one side of the heater 522 is in direct contact with the carrier 4, and the side of the heater 522 away from the carrier 4 is in direct contact with the cooling assembly 521, i.e., the cooling assembly 521, the heater 522 and the carrier 4 are in contact in turn. When the cooling assembly 521 cools the carrier 4, it first cools the heater 522 and then cools the carrier 4.

[0229] The nucleic acid amplification using the nucleic acid amplification module 10 provided in this embodiment includes the following steps:

[0230] The carrier 4 is clamped between the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56;

[0231] The cooling assembly 521 maintains continuous refrigeration to continuously cool the reaction sample by the cooling assembly 521;

[0232] The temperature increasing process increases the power of the heater 522 to heat the reaction sample to the denaturation temperature or the extension temperature;

[0233] The temperature decreasing process reduces the power of the heater 522 to cool the reaction sample to the annealing temperature

[0234] or includes the following steps:

[0235] The carrier 4 is arranged between the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56;

[0236] The cooling assembly 521 keeps cooling continuously to keep the reaction sample being cooled continuously by the cooling assembly 521;

[0237] The power of the heater 522 is increased to heat the reaction sample to the denaturation temperature;

[0238] The power of the heater 522 is controlled to keep the reaction sample at the denaturation temperature for a first preset time;

[0239] The power of the heater 522 is decreased to cool the reaction sample to the annealing temperature;

[0240] The power of the heater 522 is adjusted to keep the reaction sample at the annealing temperature for a second preset time in the low-temperature annealing stage;

[0241] The above steps are repeated for a plurality of times until a preset cycle number or a preset amplification level is reached.

[0242] The power of the heater 522 is adjusted to heat the reaction sample to the denaturation temperature or the extension temperature in the heating process.

[0243] The cooling assembly 521 keeps cooling continuously to keep the part of the heater 522 close to the cooling assembly 521 at a lower temperature all the time. When the heat of the reaction sample is transferred through the heater 522 to realize the heating or cooling of the reaction sample, since the part of the heater 522 close to the cooling assembly 521 is kept at a lower temperature all the time, the cooling assembly 521 only needs to cool another part of the heater 522 and the reaction sample when the reaction sample needs to be cooled. That is, since the volume of the heater 522 that needs to be cooled is reduced, the whole volume that needs to be cooled is reduced, thereby shortening the time required for cooling.

[0244] In the cooling, heating, low-temperature annealing, high-temperature denaturation or extension stages, the heater 522 is controlled to keep the other part of the heater 522 close to the reaction sample at a required temperature, and the reaction sample is kept, rapidly cooled or rapidly heated by controlling the heater 522, so that the process of lifting and cooling the reaction sample is controlled within 2.5s, thereby greatly shortening the detection time.

[0245] In addition, the cooling assembly 521 keeps cooling continuously, and the cooling assembly 521 has been cooled in advance and has cooled the heater 522 before the reaction sample needs to be cooled, so as to seamlessly connect with the cooling demand of the reaction sample, thereby especially improving the cooling speed.

[0246] As Figure 13 , Figure 14and Figure 15 As shown in FIG. 6, specifically, the cooling assembly 521 comprises a cooling body 5211 in which cooling liquid can flow through, and a base 5212 provided with a mounting groove in which the cooling body 5211 is mounted, and the heater 522 is in contact with the end face of the cooling body 5211.

[0247] As shown in FIG. 6, the cooling body 5211 further comprises cooling fins 52112, and a flow channel for flowing the liquid cooling medium is formed between two adjacent cooling fins 52112, and the cooling medium flows continuously to take away the heat conducted to the cooling body 5211 by the carrier 4 and the heater 522. Figure 15

[0248] Optionally, the cooling body 5211 further comprises a cooling shell 52111 provided with a cooling groove 52113, and the cooling fins 52112 are connected to the cooling shell 52111, which can be integrally formed with the cooling fins 52112 by casting or welding, and the cooling shell 52111 is arranged in the cooling groove 52113. The cooling shell 52111 can be further provided with medium openings 52116 in communication with the cooling groove 52113, and the number of the medium openings 52116 can be two, and the cooling medium enters the cooling groove 52113 through one medium opening 52116 and flows out of the cooling groove 52113 through the other medium opening 52116.

[0249] As shown in FIG. 6, the cooling assembly 521 further comprises a cover body 5217 covering the groove opening of the cooling groove 52113, and a first sealing ring 5218 can be arranged between the cover body 5217 and the cooling shell 52111 to prevent the cooling liquid in the cooling groove 52113 from leaking. Figure 13 Figure 14 As shown in FIG. 6, the opening direction of the groove opening of the cooling groove 52113 is towards the groove bottom of the mounting groove of the base 5212, so that the groove bottom of the mounting groove of the base 5212 can support the cover body 5217 after the cover body 5217 covers the groove opening of the cooling groove 52113. The end of the cooling shell 52111 away from the cover body 5217 is in contact with the heater 522. Figure 14 Figure 15 As shown in FIG. 6, the second temperature adjusting mechanism 56 further comprises a bearing plate 561 provided with a through hole 5611, and one end of the cooling assembly 521 of the second temperature adjusting mechanism 56 is arranged in the through hole 5611 (i.e. the end of the cooling shell 52111 away from the cover body 5217 is arranged in the through hole 5611), so that the end face of the cooling body 5211 extends out of the through hole 5611.

[0250] As shown in FIG. 6, the second temperature adjusting mechanism 56 further comprises a bearing plate 561 provided with a through hole 5611, and one end of the cooling assembly 521 of the second temperature adjusting mechanism 56 is arranged in the through hole 5611 (i.e. the end of the cooling shell 52111 away from the cover body 5217 is arranged in the through hole 5611), so that the end face of the cooling body 5211 extends out of the through hole 5611. Figure 13 Figure 14 ​​​​As shown, the end face of the cooling body 5211 is located on the upper side of the bearing plate 561, and it can be understood that the upper side, lower side, left side or right side and the like in the embodiment refer to the relative drawings, and not the actual use direction), and protrudes from the bearing plate 561, so that the heater 522 is placed on the end face of the cooling body 5211 of the second temperature adjusting mechanism 56, so that the cooling body 5211 can cool the heater 522.

[0251] Optionally, the cooling assembly 521 further comprises a heat preservation layer 5215, which is arranged on the cooling body 5211 and located on the side where the two cooling bodies 5211 are close to each other. The heat preservation layer 5215 can separate the cooling body 5211 from the bearing plate 561, thereby avoiding heat exchange between the cooling body 5211 and the bearing plate 561, and avoiding heat loss of the cooling body 5211.

[0252] As shown in Figures 13-15 Preferably, the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 each further comprise a second temperature detection unit 5216. A temperature measurement hole 52117 is formed in the cooling assembly 521, and the second temperature detection unit 5216 is arranged in the temperature measurement hole 52117, thereby detecting the temperature of the cooling assembly 521. The second temperature detection unit 5216 can be electrically connected to the control module, so that the control module adjusts the temperature of the cooling liquid entering the cooling groove 52113 according to the temperature of the cooling assembly 521.

[0253] As shown in Figure 15 and Figure 16 Optionally, the cooling groove 52113 of the cooling shell 52111 is connected with a temperature measurement protrusion 52118. The temperature measurement hole 52117 is formed in the cover 5217 and the temperature measurement protrusion 52118, and a second sealing ring 5219 is arranged between the temperature measurement protrusion 52118 and the cover 5217, thereby preventing the cooling liquid from entering the temperature measurement hole 52117, and thereby protecting the second temperature detection unit 5216. Optionally, the second temperature detection unit 5216 comprises a temperature sensor, and the second temperature detection unit 5216 is electrically connected to the control module 50 through the electric connection line 5214.

[0254] As shown in Figure 14As shown, optionally, multiple sets of elastic leveling components 51 are spaced apart between the drive mechanism 53 and the first temperature regulating mechanism 52. The elastic leveling components 51 are elastic to level the first temperature regulating mechanism 52, ensuring that the first temperature regulating mechanism 52 is in complete contact with the carrier 4, and preventing the first temperature regulating mechanism 52 from making hard contact with the carrier 4 and damaging the carrier 4. Of course, when the drive mechanism 53 only drives the second temperature regulating mechanism 56 to move, multiple sets of elastic leveling components 51 are spaced apart between the drive mechanism 53 and the second temperature regulating mechanism 56; or when the drive mechanism 53 drives the first temperature regulating mechanism 52 and the second temperature regulating mechanism 56 to move, multiple sets of elastic leveling components 51 are spaced apart between the drive mechanism 53 and the first temperature regulating mechanism 52, and between the drive mechanism 53 and the second temperature regulating mechanism 56.

[0255] like Figure 17 As shown, specifically, the elastic leveling component 51 is connected between the mounting bracket 532 and the first temperature regulating mechanism 52. When the first temperature regulating mechanism 52 contacts the carrier 4, if the first temperature regulating mechanism 52 is tilted relative to the carrier 4, the portion of the first temperature regulating mechanism 52 closer to the carrier 4 will contact the carrier 4 first. The portion of the first temperature regulating mechanism 52 that is not in contact with the carrier 4 can continue to move, while the portion of the first temperature regulating mechanism 52 in contact with the carrier 4 will have its corresponding elastic leveling component 51 compressed until the first temperature regulating mechanism 52 is fully in contact with the carrier 4. Thus, before the first temperature regulating mechanism 52 and the second temperature regulating mechanism 56 approach each other, whether the mounting bracket 532 is parallel to the surface of the carrier 4, and whether the first temperature regulating mechanism 52 is parallel to the surface of the carrier 4, will not affect the final contact between the first temperature regulating mechanism 52 and the surface of the carrier 4, thereby reducing the precision requirements for the mounting bracket 532, the first temperature regulating mechanism 52, and the connection between them.

[0256] like Figure 17 As shown, the elastic leveling assembly 51 includes a connecting rod 511 and an elastic element 512. The connecting rod 511 slides through the mounting frame 532, and one end of the connecting rod 511 is connected to the first temperature regulating mechanism 52. The elastic element 512 is sleeved on the connecting rod 511 and located between the mounting frame 532 and the first temperature regulating mechanism 52. The elastic element 512 can be a spring or the like. When the elastic leveling assembly 51 is compressed, the connecting rod 511 slides relative to the mounting frame 532 to reduce the length of the connecting rod 511 between the first temperature regulating mechanism 52 and the mounting frame 532. At the same time, the elastic element 512 is compressed until the first temperature regulating mechanism 52 is in complete contact with the carrier 4.

[0257] Optionally, a hole is formed on the mounting frame 532, and the end of the connecting rod 511 can be connected with the first flange 513, which abuts against the surface of the mounting frame 532 away from the first temperature adjusting mechanism 52, so as to avoid the connecting rod 511 from falling off when the first temperature adjusting mechanism 52 is away from the second temperature adjusting mechanism 56.

[0258] As shown in Figures 18-20 , a conversion plate 524 can also be arranged between the elastic leveling assembly 51 and the cooling assembly 521 of the first temperature adjusting mechanism 52, and the cooling assembly 521 and the conversion plate 524 can be fixedly connected by bolts or the like, and the elastic leveling assembly 51 and the first temperature adjusting mechanism 52 are connected through the conversion plate 524, so as to facilitate the connection of the elastic leveling assembly 51.

[0259] As shown in Figure 22 and Figure 18 , the nucleic acid amplification module 10 further comprises a pressing mechanism 58, and the first temperature adjusting mechanism 52 and / or the second temperature adjusting mechanism 56 is connected with the pressing mechanism 58 to press the pressing cavity 46 of the carrier 4 clamped on the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56. That is, by applying an external force F to the pressing cavity 46 through the pressing mechanism 58, the pressing cavity 46 is deformed, and then the pressing cavity 46 controls the pressure in the amplification cavity 41.

[0260] Specifically, as shown in Figure 20 , the first temperature adjusting mechanism 52 is connected with the pressing mechanism 58, and as shown in Figure 21 and Figure 18 , the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 are both connected with the pressing mechanism 58. Of course, in other optional embodiments, the second temperature adjusting mechanism 56 can also be connected with the pressing mechanism 58.

[0261] As shown in Figure 20 , optionally, when the first temperature adjusting mechanism 52 is connected with the pressing mechanism 58, and the carrier 4 is placed on the second temperature adjusting mechanism 56, the pressing cavity 46 can be opposite to the bearing plate 561, so that the bearing plate 561 supports the pressing cavity 46. As shown in Figure 21 and Figure 14 , when the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 are both connected with the pressing mechanism 58, the two pressing mechanisms 58 are arranged opposite to each other to press the pressing cavity 46.

[0262] As shown in Figure 18 , Figure 22 and Figure 19As shown, the following is described by way of example that the pressing mechanism 58 is arranged on the first temperature adjusting mechanism 52, and it can be understood that the connection mode of the second temperature adjusting mechanism 56 and the pressing mechanism 58 is substantially the same as the connection mode of the first temperature adjusting mechanism 52 and the pressing mechanism 58, and thus, no longer be described. Optionally, the base 5212 of the first temperature adjusting mechanism 52 is provided with a stepped mounting hole 523, the pressing mechanism 58 includes a pressing assembly and a buffer 581, the pressing assembly is connected to the stepped mounting hole 523, and the buffer 581 is connected between the pressing assembly and the stepped mounting hole 523, so that the pressing assembly is in elastic contact with the carrier 4, thereby avoiding the carrier 4 from being damaged. Optionally, the buffer 581 is a spring.

[0263] As shown in the drawings, Figure 22 Further, the pressing assembly includes a mounting rod 582 and a pressure head 583, the mounting rod 582 is slidably arranged in the stepped mounting hole 523, and a first stepped surface 5231 of the stepped mounting hole 523 can limit one end of the mounting rod 582, so as to avoid the mounting rod 582 from being separated from the stepped mounting hole 523. Specifically, one end of the mounting rod 582 can be connected to a second flange 584, so that the second flange 584 is in abutment with the first stepped surface 5231, thereby limiting the mounting rod 582. At the same time, the end of the second flange 584 away from the second stepped surface 5232 can be in abutment with the adapter plate 524, so as to limit the extreme position of the mounting rod 582 through the adapter plate 524 and the first stepped surface 5231.

[0264] The pressure head 583 is connected to the other end of the mounting rod 582 and can extend out of the stepped mounting hole 523 to press against the carrier 4, and optionally, the mounting rod 582 and the pressure head 583 are threadedly connected.

[0265] The buffer 581 is sleeved on the mounting rod 582, one end of the buffer 581 is in abutment with the second stepped surface 5232 of the stepped mounting hole 523, and the other end of the buffer 581 is in abutment with the pressure head 583, so that when the pressure head 583 presses against the carrier 4, the pressure head 583 is in elastic contact with the carrier 4, thereby avoiding the pressure head 583 from damaging the carrier 4.

[0266] The structure of the pressing mechanism 58 and the mounting manner of the pressing mechanism 58 with the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 are not limited to this, and in other optional embodiments, a through hole can be formed on the base 5212, the pressing mechanism 58 is arranged in the through hole, the pressing mechanism 58 can be connected with a cylinder or an electric cylinder or the like to drive a pressing driving part (not shown in the figure) to move the pressing mechanism 58, and optionally, the through hole extends in the first direction, and the pressing driving part drives the pressing mechanism 58 to reciprocate in the first direction. In use, before the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 clamp the carrier 4, the end of the pressing mechanism 58 close to the carrier 4 is located in the through hole, so as to facilitate the carrier 5 to be placed on the nucleic acid amplification module 10; after the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 clamp the carrier 4, the pressing driving part drives the pressing mechanism 58 to extend out of the through hole, and then the pressing cavity 46 is pressed. By pressing the pressing cavity 46 by the pressing mechanism 58, the gas and / or liquid (the liquid can be a reaction sample) in the pressing cavity 46 press the reaction sample in the amplification cavity 41, and then the reaction sample presses the corresponding cavity wall of the amplification cavity 41, so that the corresponding cavity wall of the amplification cavity 41 expands outward, so that the outside of the corresponding cavity wall of the amplification cavity 41 is in full contact with the nucleic acid amplification module 10. At the same time, the reaction sample is attached to the inner side of the cavity wall of the amplification cavity 41 under the pressure of the pressing cavity 46, and the outer side of the cavity wall of the amplification cavity 41 is in full contact with the nucleic acid amplification module 10, thereby greatly improving the amplification efficiency.

[0267] As shown in Figure 23 and Figure 22 , the nucleic acid amplification module 10 further comprises a positioning mechanism 57 connected to the first temperature adjusting mechanism 52 or the second temperature adjusting mechanism 56 and used for positioning the carrier 4. Illustratively, the positioning mechanism 57 is arranged on the bearing plate 561, and the positioning mechanism 57 is used for keeping the side wall 42 of the carrier 4 at a predetermined distance from the nucleic acid detection module 20, so as to ensure the accuracy of the fluorescence detection result.

[0268] The positioning mechanism 57 comprises a first positioning assembly used for positioning in a direction perpendicular to the insertion direction of the carrier 4 into the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56, that is, as shown in Figure 22 , in this embodiment, the first positioning assembly positions the carrier 4 in the first direction and the second direction, and the first direction and the second direction are perpendicular to the insertion direction of the carrier 4, and the insertion direction is consistent with the vertical direction.

[0269] Optionally, the first positioning assembly comprises a first positioning part 5711 and a second positioning part 5712, the first positioning part 5711 and the second positioning part 5712 are arranged in the second direction and are spaced apart from each other, so as to insert the carrier 4 between the first positioning part 5711 and the second positioning part 5712 and limit the carrier 4 between the first positioning part 5711 and the second positioning part 5712. When the carrier 4 is placed on the bearing plate 561, the position of the carrier 4 provided with the extrusion cavity 46 is located between the first positioning part 5711 and the second positioning part 5712, and the extrusion mechanism 58 is also located between the first positioning part 5711 and the second positioning part 5712, that is, the first positioning part 5711 and the second positioning part 5712 do not block the extrusion mechanism 58 from pressing against the carrier 4, so that the first positioning assembly does not interfere with the extrusion mechanism 58.

[0270] Further, the first positioning part 5711 and the second positioning part 5712 are symmetrically arranged and each comprise a first plate 5713 and a second plate 5714, the first plate 5713 and the second plate 5714 are connected to form an L-shaped structure, one end of the first plate 5713 is connected to the bearing plate 561, and the other end is connected to the second plate 5714, one second plate 5714 extends in the direction of the other first plate 5713 and is arranged in parallel with the bearing plate 561. The two first plates 5713 limit the carrier 4 in the second direction, and the two second plates 5714 and the bearing plate 561 limit the carrier 4 in the first direction.

[0271] The positioning mechanism 57 further comprises a second positioning assembly 572, the second positioning assembly 572 is arranged opposite to the nucleic acid detection module 20, and the second positioning assembly 572 and the nucleic acid detection module 20 abut the opposite two side walls 42 of the carrier 4 respectively.

[0272] Optionally, the second positioning assembly 572 comprises a fixing part 5721, an elastic adjusting part 5722 and an abutting part 5723. The fixing part 5721 is connected to the bearing plate 561, one end of the elastic adjusting part 5722 is connected to the fixing part 5721, and the other end is connected to the abutting part 5723, the abutting part 5723 is used for abutting the side wall 42 of the carrier 4, and the elastic adjusting part 5722 can be a spring or the like. Optionally, the carrier 4 is inserted between the abutting part 5723 and the fiber seat 2012 from the front end of the abutting part 5723, and the front end of the abutting part 5723 is provided with a chamfer to smoothly enter the carrier 4 between the abutting part 5723 and the fiber seat 2012. When the carrier 4 is located between the abutting part 5723 and the fiber seat 2012, the elastic adjusting part 5722 is compressed to push the abutting part 5723 to continuously abut the carrier 4.

[0273] As Figure 18As shown, optionally, the thickness of the side of the carrier 4 near the abutment portion 5723 is greater, so that the abutment portion 5723 can stably abut the carrier 4, and prevent the carrier 4 from deforming when the abutment portion 5723 abuts the carrier 4. For this purpose, the distance between the second plate 5714 and the support plate 561 needs to allow the thicker side of the carrier 4 to pass through.

[0274] The thickness of the position on the carrier 4 where the extrusion cavity 46 is located is greater than that of the position where the amplification cavity 41 is located, and is approximately the same as the thickness of the thicker side of the carrier 4. On the one hand, this allows the carrier 4 to have greater strength at the position where it is extruded by the extrusion mechanism 58, thus preventing damage to the cavity wall corresponding to the extrusion cavity 46, and also allows the extrusion cavity 46 to have greater thickness, thereby providing sufficient deformation space. On the other hand, this allows the second plate 5714 and the carrier plate 561 to position the carrier 4.

[0275] like Figure 24a and Figure 24a As shown, in order to reduce the time required to cool the heater 522, the heater 522 may optionally be a thin-layer structure with a thickness of approximately a few tenths of a millimeter to several millimeters.

[0276] like Figure 24a As shown, heater 522 includes heating element 91. A power supply is connected to heating element 91, which is a controllable heating source inside heater 522. Heating element 91 can be a resistor, such as a thin resistance wire structure made of copper. The heating power is controlled by controlling the current flowing through the resistor, thereby achieving temperature control. In other optional embodiments, heating element 91 can also be a coil structure or use ferromagnetic materials for electromagnetic induction heating.

[0277] like Figure 24a As shown, preferably, the heater 522 includes at least two independently controlled heating elements 91. The heating elements 91 can be independently controlled to improve the uniformity of the reaction sample temperature. For example, if the temperature of one heating element 91 does not reach the preset temperature (how to detect the temperature of the heating element 91 is described in detail below), the current to that heating element 91 is increased, causing the reaction sample to quickly rise to the preset temperature. In this embodiment, since the heater 522 is in direct contact with the reaction sample within the carrier 4, the heater 522 has a small thickness and high thermal conductivity with the reaction sample. The temperature of the heating elements 91 in the heater 522 can be equivalent to the temperature of the reaction sample. Therefore, controlling the temperature of each heating element 91 to reach the preset temperature ensures that the reaction sample is at the preset temperature everywhere, thereby guaranteeing the uniformity of the reaction sample temperature.

[0278] like Figure 24aAs shown, the heater 522 can further include an upper conducting assembly 92 and a lower conducting assembly 95, and the heating element 91 is clamped between the upper conducting assembly 92 and the lower conducting assembly 95. The upper conducting assembly 92 and the lower conducting assembly 95 have the functions of conducting heat and insulation.

[0279] The heater 522 includes a uniform heating layer 921, and specifically, the upper conducting assembly 92 can further include the uniform heating layer 921. The uniform heating layer 921 is in contact with the first wall 43 or the second wall 44 of the carrier 4, and the uniform heating layer 921 can ensure uniform heat conduction in the longitudinal direction and the transverse direction (i.e., the thickness direction of the reaction sample and the direction perpendicular to the thickness direction), and ensure the temperature uniformity of the sample liquid. Optionally, the upper conducting assembly 92 can further include an insulation layer 922, which can be made of high-thermal-conductivity ceramic or other insulating materials, and the uniform heating layer 921 can be made of copper or aluminum.

[0280] The lower conducting assembly 95 further includes an insulating thermal resistance layer 951. The insulating thermal resistance layer 951 has certain thermal resistance and insulation properties. In addition to insulating the heating element 91, the insulating thermal resistance layer 951 can also form a longitudinal thermal resistance. The size of the thermal resistance can be designed by selecting the material and the thickness, and can meet different design requirements, such as a thin layer with a thickness of 0.1-0.3 mm, and the material thermal conductivity is selected in the range of 0.2-0.5 W / mK.

[0281] Optionally, the lower conducting assembly 95 further includes a thermal conducting layer 952 made of copper or other thermal conducting materials, and the thermal conducting layer 952 is located on the side of the insulating thermal resistance layer 951 away from the heating element 91. Further, the thermal conducting layer 952 is the outermost layer of the lower conducting assembly 95, and directly contacts the cooling assembly 521. The thermal conducting layer 952 is made of copper or other materials with high thermal conductivity. Due to cost control or processing technology limitations, it is difficult to avoid point contact on the surface of the lower conducting assembly 95 in contact with the cooling assembly 521. When the outermost layer of the lower conducting assembly 95 is the thermal conducting layer 952, even if there is point contact between the thermal conducting layer 952 and the cooling assembly 521, the thermal conducting layer 952 can uniformly distribute heat on the entire thermal conducting layer 952 due to its good conductivity, and thus uniformly distribute heat on other layers of the lower conducting assembly 95.

[0282] Preferably, the heating element 91 of the present embodiment is a resistance wire, and there is a specific relationship between the resistance wire and its temperature. Therefore, the real-time resistance value change of the heating element 91 can be measured while heating, and the average temperature of the heating element 91 can be derived through the resistance temperature coefficient and the nominal resistance value. The temperature in real time without delay reflects the current temperature of the heater 522, and thus can be used for fast feedback control of the heater 522 and the reaction sample temperature. Compared with the prior art, the sample temperature can be more accurately controlled, and the overall reaction speed of the temperature control system can be improved.

[0283] In order to detect the resistance of the heating element 91, the heater 522 can optionally further comprise a wiring port 96 for a resistance detecting element to detect the resistance of the heating element 91 to measure the temperature of the heating element 91 by resistance temperature measurement method. The resistance detecting element can comprise a horizontal paster connector (not shown in the figure) having a plurality of connection terminals which are inserted into the wiring port 96 and electrically connected with the wiring port 96, so as to detect the voltage U and the current I of the heating element 91 through the wiring port 96, and then obtain the resistance R (R=U / I). Meanwhile, the connection terminals are electrically connected with the wiring port 96, and the heating element 91 can be powered through the wiring port 96.

[0284] Although the resistance temperature measurement method can measure the temperature of the carrier 4 without delay, the resistance temperature measurement method has a disadvantage that for the same type of resistance wire (heating element 91), such as copper resistance wire, the nominal resistance value and the resistance temperature coefficient (the resistance value at the nominal temperature is referred to as the nominal resistance value) of the resistance wires are slightly different, which may cause the real resistance temperature coefficient of a single heating element 91 to be slightly different from the nominal resistance value, which may cause temperature measurement error. Therefore, preferably, Figure 24b As shown, the heater 522 further comprises a first temperature detecting unit 99 for detecting the temperature of the heater 522, which can comprise a contact type temperature sensor or a non-contact type temperature sensor, etc. The non-contact type temperature sensor is an infrared sensor or the like which can not contact the heater 522 when measuring the temperature, such as an infrared sensor. Figure 25 As shown, the non-contact type temperature sensor can be located in the through hole 5611 to detect the temperature of the heater 522, and meanwhile, the structural compactness of the nucleic acid amplification module 10 can be improved; the contact type temperature sensor is a sensor which needs to contact the heater 522 when measuring the temperature. Although the first temperature detecting unit 99 can detect the temperature of the heater 522, since the temperature of the reaction sample changes rapidly during the amplification stage, when the first temperature detecting unit 99 detects the temperature of the heater 522, the first temperature detecting unit 99 needs a certain reaction time to measure the temperature, so that the detection result measured by the first temperature detecting unit 99 will normally have a temperature measurement delay of 1-2s. During the rapid temperature rising and falling process, the temperature change of the heater 522 can reach more than 30℃ in 1-2s, so that it is relatively difficult to control the heater 522 by the first temperature detecting unit 99 during the rapid temperature rising and falling process. The present embodiment controls the heater 522 by the double temperature measurement method of resistance temperature measurement method and temperature calibration of the heater 522 by the first temperature detecting unit 99.

[0285] This embodiment neither relies entirely on the temperature value measured by the uncalibrated resistance thermometry nor entirely on the temperature control heater 522 detected by the first temperature detection unit 99. Instead, it combines the two, using the first temperature detection unit 99 to measure the temperature of the heater 522 and the resistance thermometry to measure the temperature of the heating element 91. This allows for rapid and accurate temperature control of the heater 522, achieving the goal of accurate temperature control. This overcomes the problems of temperature detection delay and large temperature measurement error caused by commonly used temperature detection methods in the prior art.

[0286] To more clearly illustrate how the first temperature detection unit 99 calibrates the temperature detected by the resistance detection element in this embodiment, combined with... Figure 24a The diagram illustrates the calibration process of resistance thermometry using the first temperature detection unit 99 in an actual test. Before calibrating the temperature value, an initial RT temperature curve, i.e., a preset temperature curve, is established. Then, a very small current, such as less than 1 mA, is applied to the heating element 91 of the heater 522. The purpose of applying such a small current is to read the resistance of the heating element 91 without causing it to overheat.

[0287] First calibration: The first temperature detection unit 99 measures the first temperature calibration value T, and the resistance detection unit detects the first voltage U1 and the first current I1 of the heating element 91 at temperature T1. According to R=U / I, the resistance R1 of the heating element 91 at temperature T1 can be obtained.

[0288] Second calibration: Subsequently, the first temperature detection unit 99 measures the second temperature calibration value T2, and the resistance detection unit detects the second voltage U2 and the second current I2 of the heating element 91 at temperature T2. According to R=U / I, the resistance R2 of the heating element 91 at temperature T2 can be obtained.

[0289] Finally, based on the two sets of linear equations in two variables: ❑�1=❑�0(1+ 𝛼 𝑇1) and 𝑅2=𝑅0(1+𝛼 (2) 𝑇1=𝑇1-T0, 𝑇2=𝑇2-T0, 𝑅1 is the resistance value of heating element 91 at temperature T1, 𝑅2 is the resistance value of heating element 91 at temperature T2, 𝛼 is the temperature coefficient of resistance of the material, T0 is the nominal temperature, 𝑅0 is the nominal resistance value), and by obtaining the specific values ​​of 𝑅0 and 𝛼, an accurate RT curve is obtained. Subsequently, the temperature of heating element 91 measured by the resistance thermometry method can be used as feedback for accurate temperature control.

[0290] The temperature calibration value can be detected during the whole nucleic acid amplification process, and the temperature can be calibrated multiple times in the subsequent process to further improve the detection accuracy.

[0291] As shown in Figure 26 The heater 522 further comprises a temperature calibration part 93 for embodying the temperature of the heating element 91, and the first temperature detection unit 99 detects the temperature of the temperature calibration part 93. It can be understood that when the plurality of heating elements 91 are independently controlled, each heating element 91 is correspondingly provided with a temperature calibration part 93, and each heating element 91 can be detected by the resistance detection element to calibrate the heating element 91 respectively. The temperature calibration part 93 can facilitate the measurement of the temperature of the carrier 4.

[0292] As shown in Figure 24a When the first temperature detection unit 99 is a contact temperature sensor, in order to facilitate the temperature measurement of the first temperature detection unit 99, two first contacts of the first temperature detection unit 99 are in contact with two temperature calibration parts 93 respectively, and the two temperature calibration parts 93 are not conductive to each other. At this time, the heater 522 can further comprise external electrical connection contacts 97 and electrical connection leads 98, the number of the external electrical connection contacts 97 and the electrical connection leads 98 can be two, two external electrical connection contacts 97 are located on the sides away from each other of the two temperature calibration parts 93, one external connection contact is electrically connected to one temperature calibration part 93 through one electrical connection lead 98, and the other external connection contact is electrically connected to the other temperature calibration part 93 through the other electrical connection lead 98.

[0293] As shown in Figure 20 The conduction of the heat equalizing layer 921 of the upper conduction assembly 92 to the temperature calibration part 93, the temperature calibration part 93 is electrically connected to the external resistance detection element at the external electrical connection contacts 97 through the electrical connection leads 98. For example, the external resistance detection element can detect the resistance of the first temperature detection unit 99 through the external electrical connection contacts 97, and then obtain the temperature of the first temperature detection unit 99 according to the resistance. Optionally, the diameter of the electrical connection lead 98 is smaller than that of the temperature calibration part 93 and the external electrical connection contact 97, so that the heat loss of the temperature calibration part 93 through the electrical connection lead 98 is reduced, and the temperature calibration part 93 can better embody the temperature of the upper conduction assembly 92, such as the heat equalizing layer 921 of the upper conduction assembly 92. The first temperature detection unit 99 realizes good electrical and thermal contact with the temperature calibration part 93 through the welding point. When the temperature of the upper conduction assembly 92, such as the heat equalizing layer 921 of the upper conduction assembly 92, changes, the first temperature detection unit 99 can quickly and accurately perceive the temperature change. The change of the resistance of the first temperature detection unit 99 caused by the temperature change can be detected in real time at the external electrical connection contacts 97, so that real-time temperature detection can be realized.

[0294] AsFigure 2b As shown, optionally, to shorten the time for the temperature of the temperature calibration portion 93 to be consistent with the temperature of the heating element 91, the heater 522 can further comprise a rapid conduction portion 94 for conducting the heat of the heating element 91 to the temperature calibration portion 93. Specifically, in the present embodiment, the heat of the heating element 91 is indirectly conducted to the temperature calibration portion 93, for example, the heating element 91 heats the heat-distribution layer 921, and the heat of the heat-distribution layer 921 is conducted to the temperature calibration portion 93 through the rapid conduction portion 94, so that the temperature calibration portion 93 accurately reflects the temperature of the heat-distribution layer 921, and the first temperature detection unit 99 can accurately measure the temperature of the heat-distribution layer 921. Since the thickness of the reaction sample is very small, the temperature of the reaction sample is basically consistent with the temperature of the heat-distribution layer 921, so the temperature of the reaction sample can be obtained by detecting the temperature of the temperature calibration portion 93.

[0295] Preferably, one side of the rapid conduction portion 94 is connected to the side of the upper conduction assembly 92 close to the heating element 91 or to the side of the lower conduction assembly 95 close to the heating element 91, and the other side is connected to the temperature calibration portion 93. The lower surface of the upper conduction assembly 92 and the upper surface of the lower conduction assembly 95 are closest to the heating element 91, and their temperatures first approach the temperature of the heating element 91, so the arrangement of the rapid conduction portion 94 can make the temperature of the rapid conduction portion 94 consistent with the temperature of the heating element 91 in the shortest time. Optionally, the rapid conduction portion 94 is made of a material with high thermal conductivity, such as metal materials such as copper or aluminum, or thermally conductive ceramics, etc. The thermal conductivity of the rapid conduction portion 94 is particularly superior to that of the lower conduction assembly 95, so as to quickly transfer heat to the temperature calibration portion 93.

[0296] The rapid conduction portion 94 comprises a patch 941 and one or more guide columns 942. The patch 941 is attached to the side of the upper conduction assembly 92 close to the heating element 91 or to the side of the lower conduction assembly 95 close to the heating element 91. One end of the one or more guide columns 942 is connected to the patch 941, and the other end is arranged in the lower conduction assembly 95 and connected to the temperature calibration portion 93. The lower surface of the upper conduction assembly 92 and the upper surface of the lower conduction assembly 95 are closest to the heating element 91, and their temperatures first approach the temperature of the heating element 91, so the arrangement of the patch 941 can make the temperature of the rapid conduction portion 94 consistent with the temperature of the heating element 91 in the shortest time. The patch 941 can increase the contact area of the rapid conduction portion 94 with the upper conduction assembly 92 or the lower conduction assembly 95, and improve the conduction efficiency. The cross-sectional area of the guide column 942 can be smaller than the cross-sectional area of the patch 941, so as to quickly conduct the temperature of the patch 941 to the temperature calibration portion 93. Optionally, the patch 941 and the guide column 942 are made of a material with high thermal conductivity, such as copper. When the patch 941 and the guide column 942 need to be made of an insulating material to avoid short circuit of the heater 522, the patch 941 or the guide column 942 can be made of a material with high thermal conductivity, such as high-thermal-conductivity ceramics.

[0297] It can be understood that the temperature calibration part 93 can be arranged one by one with the patch 941, and two temperature calibration parts 93 can also be connected on one patch 941. One temperature calibration part 93 can be connected with one guide column 942, and in order to improve the temperature uniformity of the temperature calibration part 93, the temperature calibration part 93 can also be connected with multiple guide columns 942.

[0298] In the embodiment, the wiring port 96 enables the heater 522 to realize its own temperature measurement function. Compared with the conventional structure which can only measure the temperature through an external temperature measurement unit, the embodiment can directly measure the temperature of the heater 522 itself, so that the temperature measurement is more accurate and fast, and the accuracy and control speed of the temperature control system can be improved.

[0299] The working process of the nucleic acid amplification module 10 provided in the embodiment is as follows: the transfer module 60 inserts the carrier 4 into the positioning mechanism 57, the driving mechanism 53 drives the first temperature adjusting mechanism 52 to move to the side where the second temperature adjusting mechanism 56 is located, until the first temperature adjusting mechanism 52 and the second temperature adjusting mechanism 56 clamp the carrier 4, at this time, the extrusion mechanism 58 is pressed to the position where the carrier 4 is provided with the extrusion cavity 46, and then the pressure in the amplification cavity 41 is adjusted. The heater 522 and the cooling assembly 521 are turned on and off under the control of the control module 50, so as to realize the amplification of the reaction sample in the carrier 4, the first temperature adjusting mechanism 52 moves away from the second temperature adjusting mechanism 56, and the transfer module 60 takes out the carrier 4. If the PCR automation all-in-one machine is used for the first time or multiple times, the heater 522 can also be calibrated in temperature.

[0300] As shown in Figure 27 and Figure 28 Optionally, at least part of the side wall 42 of the amplification cavity 41 is light-transmitting, and the nucleic acid detection module 20 detects the reaction sample through the light-transmitting side wall 42 of the carrier 4. Optionally, the material of the light-transmitting side wall 42 can be polydimethylsiloxane (i.e., PDMS), polypropylene (i.e., PP) or polycarbonate (i.e., PC), and PDMS, PP and PC are optical transparent materials with good biocompatibility.

[0301] The existing PCR automation machine often chooses a larger area for nucleic acid detection, so that the reaction sample area receiving excitation light is large, the generated fluorescence signal is strong, and accurate detection results can be easily obtained. According to the above thinking habit, since the area of the first wall 43 or the second wall 44 in the embodiment is large, it is often considered to perform fluorescence detection through the first wall 43 or the second wall 44. However, in the embodiment, the temperature adjusting mechanism is arranged on both sides of the carrier 4 to realize rapid temperature rise and fall, and the nucleic acid detection module 20 performs fluorescence detection on the side of the carrier 4. While realizing rapid temperature rise and fall and fluorescence detection, compared with the way of performing fluorescence detection through the first wall 43 or the second wall 44, the structure of the PCR automation machine is more compact, the occupied space is reduced, and the detection efficiency is further improved.

[0302] As shown in Figure 27 The fluorescence detection light path in the prior art is a whole structure, that is, the excitation light path 200 and the fluorescence signal light path 201 of the fluorescence detection light path are transmitted through one optical fiber, so that the excitation light emitted by the light source 205 enters the fluorescence signal light path 201 after being reflected by the optical fiber. The dichroic mirror 203 and the optical filter 204 are arranged on the path of the optical fiber transmission, and the dichroic mirror 203 and the optical filter 204 both allow the fluorescence signal to pass through and filter the excitation light in the fluorescence signal light path 201. However, since the dichroic mirror 203 and the optical filter 204 cannot filter 100% of the excitation light and cannot allow 100% of the fluorescence signal to pass through, that is, the fluorescence signal will be attenuated when passing through the dichroic mirror 203 and the optical filter 204, and part of the filtered excitation light will enter the detection circuit 206 for detecting the fluorescence signal. In the prior art, since the area of the reaction sample excited by the excitation light is large, the number of generated fluorescence signals is also large, therefore, the excitation light and the attenuated fluorescence signal that pass through the optical filter have little effect on the detection results, and relatively accurate detection results can be obtained.

[0303] However, the thickness of the amplification cavity 41 in the embodiment is thin (0.3-0.6 mm), and when the fluorescence detection is performed through the side wall 42 of the amplification cavity 41, the reaction sample that can receive excitation light is greatly reduced, so the number of fluorescence signals excited each time is about one order of magnitude less. If the existing fluorescence detection light path is used for detection, the signal detected by the detection circuit is low, the excitation light that passes through the optical filter will drown out the fluorescence signal, and thus the error of the detection will be large, and even the fluorescence detection circuit cannot detect the fluorescence signal.

[0304] As shown in Figure 27The nucleic acid detection module 20 provided in the embodiment comprises a fluorescence detection light path system 201, and the fluorescence detection light path system 201 comprises at least one fluorescence emission unit 1, which is used to emit excitation light. Optionally, in the embodiment, at least two fluorescence emission units 1 are arranged, and the at least two fluorescence emission units 1 are respectively used to emit excitation light. The control module 50 controls the at least two fluorescence emission units 1 to respectively emit excitation light in different time periods. Of course, the number of the fluorescence emission units 1 can also be one. For example, as shown in Figure 27 the drawing, four fluorescence emission units 1 can be arranged. Of course, the number of the fluorescence emission units 1 is not limited to four, and can be less than four or more than four.

[0305] Optionally, the control module 50 is arranged on the rack 40. The control module 50 is also electrically connected with the nucleic acid amplification module 10 and the nucleic acid detection module 20, so as to control the nucleic acid amplification module 10 and the nucleic acid detection module 20. The control module 50 can also be electrically connected with other modules, so as to respectively control the modules. It can be understood that the control module 50 can be a centralized or distributed control module 50. For example, the control module 50 can be a single microcontroller, or can be composed of multiple distributed microcontrollers. The microcontroller can run a control program, so as to control components to realize their functions. The control module 50 can be connected with the modules through a line or through wireless connection such as Bluetooth.

[0306] The fluorescence emission unit 1 is independently arranged, and the excitation light transmitted in the fluorescence emission unit 1 will not be reflected into the light path for transmitting fluorescence signals, so that the amount of excitation light in the light path of the fluorescence signals is greatly reduced, the background of the fluorescence signals is greatly reduced, and the detection accuracy is improved. Meanwhile, when the reaction sample in the carrier 4 has multiple fluorescence probes / dyes, the controller can control different fluorescence emission units 1 to be sequentially turned on in different time periods. For example, the first fluorescence emission unit 1 works in the first to second seconds after detection starts, the second fluorescence emission unit 1 works in the second to third seconds after detection starts, and so on. Only one fluorescence emission unit 1 works in one time period, and one fluorescence emission unit 1 only needs to emit one kind of excitation light. Therefore, only one kind of excitation light is excited in one time period, and only one kind of fluorescence signal is generated. When the fluorescence signal is detected, the noise generated by the excitation light is small, and the accuracy of the detection result can be further improved. In addition, the controller controls the conduction of the fluorescence emission units 1 in each channel, so that only one excitation light exists at the same time, the excitation light channel is switched in millisecond level, and the reaction sample can be quickly detected.

[0307] Optionally, the wavelengths of the excitation light emitted by the at least two fluorescence emission units 1 are different from each other, so as to improve the utilization rate of the fluorescence emission units 1. Of course, in order to avoid the inaccuracy of the detection result caused by the failure of the fluorescence emission units 1, a standby fluorescence emission unit 1 can also be provided, for example, the wavelengths of the excitation light emitted by two fluorescence emission units 1 among the at least two fluorescence emission units 1 are the same.

[0308] As shown in Figure 27 , the fluorescence emission unit 1 comprises a light source 12 and an excitation light fiber 11. The light source 12 can be an LED or the like, and the light source 12 is used to emit excitation light. The light source 12 is electrically connected with the control module 50. The excitation light fiber 11 is used to transmit the excitation light emitted by the light source 12, so as to transmit the excitation light to the reaction sample. The light source 12 and the reaction sample are connected through the excitation light fiber 11. The excitation light fiber 11 facilitates the isolation of the heat source, so that the experimental data is more stable. In addition, the connection between the excitation light fiber 11 and the light source 12 is simple, which is beneficial to the anti-vibration. At the same time, since the size of the optical fiber itself is small, it can meet the needs of the flat structure of the carrier 4. In addition, one light source 12 corresponds to one excitation light fiber 11, and each excitation channel has a separate optical fiber, which is easy to couple and has small light loss.

[0309] As shown in Figure 29 , the fluorescence detection light path system 201 further comprises a fluorescence detection unit 2. The fluorescence detection unit 2 is used to detect the fluorescence signal. The fluorescence emission unit 1 and the fluorescence detection unit 2 receiving the excitation signal are completely separated, which reduces the background of the fluorescence signal and improves the detection sensitivity of the fluorescence detection light path system 201. At the same time, the fluorescence emission unit 1 and the fluorescence detection unit 2 are completely separated, which reduces the optical elements such as dichroic mirrors in the fluorescence detection unit 2, reduces the cost, and improves the detection efficiency of the fluorescence.

[0310] As shown in Figure 27 , when the number of the fluorescence detection unit 2 is one, the fluorescence detection unit 2 comprises one detector 22, one fluorescence transmission light path 21 and a turntable 23. A plurality of optical filters 211 are arranged on the turntable 23. The fluorescence transmission light path 21 comprises one collection optical fiber 212. A plurality of fluorescence signals are transmitted to the optical filters 211 through the one collection optical fiber 212. The plurality of optical filters 211 can pass one kind of fluorescence signal respectively. The channels are switched by rotating the turntable 23, so that different fluorescence signals pass through and are accepted by the detector 22.

[0311] At the same time, a more preferred scheme of the fluorescence detection unit 2 is provided in the embodiment, as shown in Figure 30 and Figure 27As shown, the fluorescence detection unit 2 includes at least two fluorescence transmission light paths 21, and the at least two fluorescence transmission light paths 21 correspond to the at least two fluorescence emission units 1 one by one, that is, one fluorescence emission unit 1 can correspond to one fluorescence transmission light path 21. The reaction sample at the opposite position of the excitation fiber 11 of the fluorescence emission unit 1 can receive stronger excitation light, and thus the reaction sample at the position can generate higher fluorescence signals. The at least two fluorescence transmission light paths 21 correspond to the at least two fluorescence emission units 1 one by one, so that the fluorescence transmission light path 21 can be arranged close to the reaction sample excited by the fluorescence emission unit 1 corresponding to the fluorescence transmission light path 21, and thus more fluorescence signals enter the fluorescence transmission light path 21, further improving the accuracy of the detection result.

[0312] Each fluorescence transmission light path 21 includes a filter 211 allowing a preset fluorescence signal to pass, that is, only a specific fluorescence signal can pass in each fluorescence transmission light path 21. Compared with the above-mentioned arrangement of only one fluorescence transmission light path 21, the arrangement of the filter 211 in each fluorescence transmission light path 21 can reduce the time required for mechanical switching when the fluorescence signal passes through the light path, realize rapid detection of the fluorescence signal, and also eliminate the influence of mechanical vibration caused by mechanical switching on the detection result.

[0313] As shown in Figure 30 and Figure 29 The fluorescence detection unit 2 further includes a detector 22, and the plurality of fluorescence transmission light paths 21 are connected to the detector 22, and the detector 22 is used for detecting fluorescence signals. The detector 22 includes a silicon photomultiplier (SiPM), a photon type detector (PD), or a photomultiplier (PMT). The fluorescence detector 22 using the silicon photomultiplier, the photon type detector, or the photomultiplier has high sensitivity, and realizes millisecond-level ultrafast and high-sensitivity detection of fluorescence signals during fluorescence detection.

[0314] Preferably, the number of the detector 22 is one, and the detector 22 is electrically connected to the control module 50 to record the intensity of the fluorescence signal in time periods. Different fluorescence transmission light paths 21 pass through different filters 211 and converge on one detector 22. Different fluorescence emission units 1 are opened in time periods by the fluorescence emission unit 1, so as to realize switching of the excitation channel, thereby exciting different signal fluorescence in time periods. The corresponding signal fluorescence can pass through the filter 211 corresponding thereto. Although the fluorescence detector 22 can only detect the intensity of the signal fluorescence and cannot detect the type of the signal fluorescence, the type of the signal fluorescence can be determined according to the acceptance time period, and thus the reaction sample corresponding to the signal fluorescence is determined. Figure 27Compared with the scheme, the above technical scheme does not need a motor to drive the rotating disc 23 to rotate, has no mechanical switching, avoids vibration caused by the rotation of the rotating disc 23, and improves the accuracy of the detection result. According to the time period, the signal fluorescence is determined, the switching between the fluorescence channels is fast, the detection speed is fast, the detection efficiency is high, the equipment cost is low, and the equipment structure is simple.

[0315] As shown in Figure 23 and in combination with Figure 31 , the fluorescence transmission light path 21 includes a collection optical fiber 212, an emission end of the excitation optical fiber 11 for emitting excitation light, and an emission end of the collection optical fiber 212 for emitting fluorescence signals to form an optical fiber group 2011.

[0316] As shown in Figure 32 and Figure 23 , and in combination with Figure 31 , the fluorescence detection light path system 201 further includes an optical fiber seat 2012, and the optical fiber group 2011 is arranged in the optical fiber seat 2012. As shown in Figure 32 and Figure 23 , the optical fiber seat 2012 includes a seat body 2013 and a flat groove 2014 formed on one side of the seat body 2013, and the optical fiber group 2011 is arranged in the flat groove 2014. In combination with Figure 27 , the second positioning assembly 572 is arranged opposite to the optical fiber seat 2012, and the second positioning assembly 572 and the optical fiber seat 2012 respectively abut against opposite two side walls 42 of the carrier 4.

[0317] The optical fibers in the optical fiber group 2011 are arranged along a preset direction, and the preset direction is the radial direction of the optical fibers (as shown by the arrow R in Figure 23 , the direction of the radial direction of the optical fibers), and in this embodiment, the preset direction is also the vertical direction (as shown in Figure 23 ), so that the emission end of the collection optical fiber 212 and the emission end of the excitation optical fiber 11 are arranged flat in the optical fiber seat 2012, as shown in Figure 33 , that is, the optical fibers (the collection optical fiber 212 and the excitation optical fiber 11) are arranged flat at one end close to the side wall 42 of the carrier 4, and the preset direction is the length direction of the side wall 42, and in the thickness direction of the carrier 4, the optical fibers in the optical fiber seat 2012 are arranged in a single layer, thereby adapting to the flat structure of the carrier 4. It can be understood that the diameter of the optical fibers can be smaller than the thickness of the carrier 4, so that the optical fibers in the optical fiber seat 2012 do not protrude from the carrier 4 in the thickness direction of the carrier 4, and therefore, the excitation light emitted by the collection optical fiber 212 can all enter the amplification cavity 41, and the entire excitation optical fiber 11 can collect fluorescence. The excitation optical fiber 11 and the collection optical fiber 212 are arranged in parallel, so that the optimal sensitivity and signal-to-noise ratio can be obtained. At the same time, when the optical fiber group 2011 is coupled with the carrier 4, the preset direction of the arrangement of the optical fibers is perpendicular to the thickness direction of the carrier 4, and the optical fiber group 2011 and the optical fiber seat 2012 are both flat structures, thereby cooperating with the carrier 4.

[0318] As shown in Figure 33 , when at least two fluorescent transmission light paths 21 and at least two fluorescent emission units 1 are provided, at least two groups of optical fiber groups 2011 are formed in correspondence, and the at least two groups of optical fiber groups 2011 in the optical fiber seat 2012 are arranged in sequence along the preset direction, that is, when a plurality of optical fiber groups 2011 are provided, the plurality of optical fiber groups 2011 are also arranged in a flat manner, and the optical fibers in the optical fiber seat 2012 are arranged in a single layer in the thickness direction of the carrier 4, so as to adapt to the flat structure of the carrier 4, and the optical fibers in the optical fiber seat 2012 do not protrude from the carrier 4 in the thickness direction of the carrier 4, so that the excitation light emitted by the collection optical fiber 212 can all enter the amplification cavity 41, and the whole excitation optical fiber 11 can collect the fluorescent light.

[0319] At the same time, the plurality of optical fiber groups 2011 are arranged in sequence, the collection optical fiber 212 and the excitation optical fiber 11 in the same group of optical fiber groups 2011 are adjacent, and the reaction sample corresponding to the excitation optical fiber 11 can generate more fluorescent signals, and the collection optical fiber 212 is close to the excitation optical fiber 11 corresponding thereto, so that more fluorescent signals enter the fluorescent transmission light path 21, and the accuracy of the detection result is further improved.

[0320] As shown in Figure 33 , one optical fiber group 2011 includes at least two collection optical fibers 212, and at least one collection optical fiber 212 is provided on both sides of the excitation optical fiber 11 in the preset direction in one optical fiber group 2011. In one optical fiber group 2011, the number of collection optical fibers 212 is increased to at least two, and the reaction sample is detected at multiple points, so as to effectively improve the detection efficiency of the fluorescent light, solve the problem of low signal, and reduce the requirement for the sensitivity of the detector 22. At the same time, when one of the collection optical fibers 212 is affected by the bubbles in the reaction sample, the detection result of the other collection optical fiber 212 can be used for correction. For example, one collection optical fiber 212 is provided on each side of the excitation optical fiber 11, and one optical fiber group 2011 is formed, that is, each fluorescent light can be collected by two collection optical fibers 212. Of course, only one collection optical fiber 212 or more than two collection optical fibers 212 can be provided in one optical fiber group 2011.

[0321] As shown in Figure 34 , the fluorescent emission unit 1 can be provided only on one side of the carrier 4, or, as shown in ​As shown, the fluorescent emission unit 1 is arranged on both sides of the carrier 4, and the excitation optical fiber 11 of the fluorescent emission unit 1 is correspondingly provided with a collection optical fiber 212. Each collection optical fiber 212 on both sides of the carrier 4 is preferably connected to a detector 22, and of course, the collection optical fiber 212 on each side can be connected to a detector 2222, respectively. Detecting both sides of the reaction sample can effectively improve the detection efficiency of fluorescence, solve the problem of low signal, and reduce the requirement for the sensitivity of the detector 22. Moreover, when the detection result of one side of the collection optical fiber 212 is inaccurate due to the shielding of bubbles in the reaction sample, the detection result of the other side can be used for correction. Of course, detection can also be performed on three or more sides of the carrier 4, and preferably, fluorescence detection is performed through the light-transmitting side wall 42 of the carrier 4.

[0322] For example, when the carrier 4 and the reagent carrying part 1001 are in an integrated structure, and the thickness direction of the carrier 4 is consistent with the vertical direction, the working process of the PCR automatic integrated machine provided in the embodiment is as follows:

[0323] The artificial or automated equipment places the reagent box 100 on the bracket 311;

[0324] The control module 50 controls the pipetting sub-module 32 to configure the sample and the reagent in the reagent box 100 into a reaction sample;

[0325] The cutting module 70 cuts the reagent box 100 to separate the carrier 4 and the reagent carrying part 1001, the turning module 7 turns the carrier 4 by 90°, or the bending module 80 bends and breaks the reagent box 100;

[0326] The control module 50 controls the transfer module 60 to transfer the carrier 4 to the nucleic acid amplification module 10, and the nucleic acid amplification module 10 works to realize amplification of the reaction sample;

[0327] The control module 50 controls the nucleic acid detection module 20 to detect the reaction sample;

[0328] The control module 50 controls the transfer module 60 to transfer the reagent carrying part 1001 to the recycling module 90;

[0329] The control module 50 controls the transfer module 60 to take out the carrier 4 from the nucleic acid amplification module 10, and transfer the carrier 4 to the recycling module 90.

[0330] For example, when the carrier 4 and the reagent carrying part 1001 are in an integrated structure, and the thickness direction of the carrier 4 is consistent with the vertical direction, the working process of the PCR automatic integrated machine provided in the embodiment can also be as follows:

[0331] The artificial or automated equipment places the reagent box 100 on the bracket 311;

[0332] The control module 50 controls the pipetting sub-module 32 to configure the sample and reagent in the reagent box 100 into a reaction sample;

[0333] The folding module 80 folds the carrier 4 by 90°, but the reagent box 100 is not broken;

[0334] The control module 50 controls the transfer module 60 to transfer the reagent box 100 to the nucleic acid amplification module 10, and the nucleic acid amplification module 10 works to realize amplification of the reaction sample;

[0335] The control module 50 controls the nucleic acid detection module 20 to detect the reaction sample;

[0336] The control module 50 controls the transfer module 60 to take out the carrier 4 from the nucleic acid amplification module 10, and transfer the carrier 4 to the recovery module 90.

[0337] For example, when the carrier 4 and the reagent carrying part 1001 are in a split structure, the working process of the PCR automatic integrated machine provided by the embodiment is as follows:

[0338] The artificial or automatic equipment places the reagent box 100 on the bracket 311;

[0339] The control module 50 controls the pipetting sub-module 32 to configure the sample and reagent in the reagent box 100 into a reaction sample;

[0340] The turning module 7 turns the carrier 4 by 90° (when the thickness direction of the carrier 4 is consistent with the vertical direction, the step is included; when the thickness direction of the carrier 4 is perpendicular to the vertical direction, the step is not included);

[0341] The control module 50 controls the transfer module 60 to transfer the carrier 4 to the nucleic acid amplification module 10, and the nucleic acid amplification module 10 works to realize amplification of the reaction sample;

[0342] The control module 50 controls the nucleic acid detection module 20 to detect the reaction sample;

[0343] The control module 50 controls the transfer module 60 to transfer the reagent carrying part 1001 to the recovery module 90;

[0344] The control module 50 controls the transfer module 60 to take out the carrier 4 from the nucleic acid amplification module 10, and transfer the carrier 4 to the recovery module 90.

[0345] Although the present application has been described in detail with general description, specific embodiments and experiments, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, are within the scope of the present application.

Claims

1. A PCR automation all-in-one machine, characterized in that, The PCR automatic machine comprises a rack, a nucleic acid amplification module and a nucleic acid detection module arranged on the rack, the nucleic acid amplification module is used for amplifying a reaction sample, and the nucleic acid detection module is used for detecting the reaction sample. The PCR automatic machine further comprises a preparation module arranged on the rack and used for preparing the sample and the reagent into the reaction sample, and the preparation module injects the reaction sample into the carrier. The preparation module comprises a carrying sub-module. The PCR automatic machine further comprises a transfer module arranged on the rack and used for transferring the carrier between the carrying sub-module and the nucleic acid amplification module, the reaction sample is contained in the carrier, the carrier has a flat structure, and the nucleic acid amplification module and / or the nucleic acid detection module vertically inserts the carrier. The PCR automatic machine further comprises a cutting module arranged on the rack and used for cutting the reagent box between the carrier of the reagent box and the reagent carrying part of the reagent box. The PCR automatic machine further comprises a control module used for controlling the nucleic acid amplification module and the nucleic acid detection module. The nucleic acid detection module comprises a fluorescence detection light path system, and the fluorescence detection light path system comprises: At least one fluorescence emission unit used for emitting excitation light. The nucleic acid amplification module comprises at least one set of temperature adjusting mechanisms capable of changing the temperature of the reaction sample in the carrier. The nucleic acid amplification module comprises two sets of temperature adjusting mechanisms, which are a first temperature adjusting mechanism and a second temperature adjusting mechanism, respectively, the positions of the first temperature adjusting mechanism and / or the second temperature adjusting mechanism are adjustable, and the first temperature adjusting mechanism and / or the second temperature adjusting mechanism are adjusted to be close to or away from each other and to clamp the carrier between the first temperature adjusting mechanism and the second temperature adjusting mechanism. The nucleic acid amplification module further comprises an extrusion mechanism connected with the first temperature adjusting mechanism and / or the second temperature adjusting mechanism to extrude the extrusion cavity of the carrier clamped on the first temperature adjusting mechanism and the second temperature adjusting mechanism.

2. The PCR automation system of claim 1, wherein, The preparation module comprises a pipetting sub-module arranged on the upper side of the carrying sub-module, and the carrying sub-module and the pipetting sub-module are relatively movable, so that the pipetting sub-module transfers the solution on the carrying sub-module and injects the reaction sample into the reagent box.

3. The PCR automation system of claim 2, wherein, The pipetting sub-module comprises a pipetting gun, and at least one of the pipetting gun and the carrying sub-module is movable in a horizontal plane and at least one of the pipetting gun and the carrying sub-module is movable in a vertical direction.

4. The PCR automation system of claim 2, wherein, The carrying sub-module can carry the reagent box, and the pipetting sub-module is used for transferring the solution between the cavities of the reagent box.

5. The PCR automation system of claim 2, wherein, The carrying sub-module comprises a bracket, and a containing part is arranged on the bracket.

6. The PCR automation system of claim 5, wherein, The containing part comprises a plurality of containing grooves, and the reagent carrying part of the reagent box can be inserted into the containing grooves.

7. The PCR automation system of claim 6, wherein, At least one of the containing grooves is provided with a heating structure for heating the reaction sample in the reagent carrying part.

8. The PCR automation all-in-one machine according to claim 2, wherein, The transfer module is used for transferring the reagent box between the carrier submodule and the nucleic acid amplification module, or the transfer module is used for transferring the reagent box or carrier between the carrier submodule, the nucleic acid amplification module and the nucleic acid detection module.

9. The PCR automation system of claim 1, wherein, The PCR automatic integrated machine further comprises a turnover module arranged in the rack, and the turnover module is used for turning over the carrier of the reagent box by a preset angle.

10. The PCR automation system of claim 1, wherein, The PCR automatic integrated machine further comprises a bending module arranged in the rack, and the bending module is used for bending one end of the reagent box by a preset angle, or the bending module is used for bending the carrier of the reagent box by a preset angle and breaking the reagent box between the carrier and the reagent carrying part.

11. The PCR automation system of claim 1, wherein, The number of the fluorescence emission units is at least two, and the control module controls at least two fluorescence emission units to emit excitation light at different time periods, respectively.

12. The PCR automation system of claim 11, wherein, The fluorescence detection optical path system further comprises: The fluorescence detection optical path system further comprises:

13. The PCR automation system of claim 12, wherein, The fluorescence detection optical path system further comprises:

14. The PCR automation system of claim 13, wherein, The number of the detector is one, and the detector is electrically connected with the control module to record the intensity of the fluorescence signal in time periods.

15. The PCR automation system of claim 14, wherein, The fluorescence emission unit comprises a light source and an excitation optical fiber, the light source is used for emitting excitation light, the light source is electrically connected with the control module, and the excitation optical fiber is used for transmitting the excitation light emitted by the light source to transmit the excitation light to the reaction sample.

16. The PCR automation system of claim 12, wherein, The fluorescence detection optical path system further comprises an optical fiber seat, the fluorescence transmission optical path comprises a collection optical fiber, an emission end of the excitation optical fiber emitting the excitation light, and an emission end of the collection optical fiber emitting the fluorescence signal forming an optical fiber group, the optical fiber group is arranged in the optical fiber seat, the optical fibers in the optical fiber seat are arranged along a preset direction, the preset direction is the radial direction of the optical fibers, so that the emission end of the collection optical fiber and the emission end of the excitation optical fiber are arranged flatly in the optical fiber seat.

17. The PCR automation system of claim 16, wherein, At least two fluorescence transmission optical paths and at least two fluorescence emission units form at least two groups of optical fiber groups, and at least two groups of optical fiber groups are arranged in sequence along the preset direction.

18. The PCR automation system of claim 17, wherein, One optical fiber group comprises at least two collection optical fibers, and at least one collection optical fiber is arranged on both sides of the preset direction of the excitation optical fiber in one optical fiber group.

19. The PCR automation system of claim 1, wherein, The nucleic acid amplification module comprises a driving mechanism, and the driving mechanism drives the first temperature adjusting mechanism and / or the second temperature adjusting mechanism to move close to or away from each other.

20. The PCR automation system of claim 19, wherein, A plurality of elastic leveling assemblies are arranged between the driving mechanism and the first temperature adjusting mechanism and / or the second temperature adjusting mechanism.

21. The PCR automation system of claim 1, wherein, The first temperature adjusting mechanism and / or the second temperature adjusting mechanism are provided with a stepped mounting hole, the extrusion mechanism comprises an extrusion assembly and a buffer, the extrusion assembly is connected to the stepped mounting hole, and the buffer is connected between the extrusion assembly and the stepped mounting hole, so that the extrusion assembly is in elastic contact with the carrier.

22. The PCR automation system of claim 1, wherein, The first temperature adjusting mechanism and the second temperature adjusting mechanism each comprise a cooling assembly for cooling the carrier.

23. The PCR automation system of claim 22, wherein, The first temperature adjusting mechanism and the second temperature adjusting mechanism each further comprise a heater arranged on a side of the cooling assembly of the first temperature adjusting mechanism and the second temperature adjusting mechanism which are close to each other.

24. The PCR automation system of claim 23, wherein, The heater comprises a heating piece, and the PCR automation all-in-one machine further comprises a resistance detection piece for detecting the resistance of the heating piece.

25. The PCR automation system of claim 24, wherein, The PCR automation all-in-one machine further comprises a first temperature detection unit for detecting the temperature of the heater.

26. The PCR automation system of claim 1, wherein, The nucleic acid amplification module further comprises a positioning mechanism for positioning the carrier, and the positioning mechanism is connected to the first temperature adjusting mechanism or the second temperature adjusting mechanism.

27. The PCR automation system of claim 1, wherein, The flat structure means that the ratio of the size of the carrier in the direction perpendicular to the thickness direction to the size in the thickness direction is greater than 5:

1.

28. The PCR automation system of claim 27, wherein, The ratio of the size is 50:1~100:1.

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