An anti-wrinkle composition and its preparation method and application

Abstract

The present disclosure provides an anti-wrinkle composition and a preparation method and application thereof, belonging to the field of cosmetic technology. The anti-wrinkle composition of the present disclosure comprises the following components in parts by weight: 0.1-1.5 parts of polysaccharide from Laveria sutchuenensis, 0.1-70 parts of extract from Heliothis radiata, 10-230 parts of butylene glycol, and 50-600 parts of water. The anti-wrinkle composition of the present disclosure improves the effects of hydration, moisturizing, improving skin elasticity and anti-wrinkle of the anti-wrinkle composition through the synergistic effect of polysaccharide from Laveria sutchuenensis and extract from Heliothis radiata.

Description

Technical Field

[0001] The present disclosure relates to the technical field of cosmetics, and in particular to an anti-wrinkle composition and a preparation method and application thereof. Background Art

[0002] As we age, our metabolism slows down, our water content and subcutaneous fat decrease, elastic fibers become thicker, our skin wrinkles, and our luster fades. Skin aging is a prominent manifestation of aging of the entire body, especially on the face. The number of fibroblasts in the body decreases, and the collagen and elastin they secrete also decrease and break due to oxidation, which causes the epidermis to lose its support, resulting in uneven collapse. The dermis begins to thin, the skin's elasticity deteriorates, and wrinkles begin to appear. In addition to natural physiological factors, the aging of human skin is also related to factors such as long-term sleep quality, ultraviolet radiation, working environment, living habits, nutritional status, and skin care.

[0003] Although skin aging is an irreversible physiological phenomenon, the aging process can be slowed down through medical beauty and the use of cosmetics. The current wrinkle removal methods on the market are as follows: (1) By injecting a small amount of botulinum toxin type A, this method may cause serious complications and pose a serious threat to skin health; (2) Using peptides and peptide combinations to achieve firming and anti-wrinkle effects, but the active ingredients cannot be well absorbed by the skin and only stay on the surface, resulting in a relatively poor anti-wrinkle effect; (3) Using polydimethylsiloxane to fill the pores and gaps in the skin to achieve an immediate wrinkle removal effect, but this method only treats the symptoms and not the root cause. Long-term use will clog the pores, irritate the skin, and cause acne or allergies; (4) Using retinol products for anti-wrinkle, although the effect is relatively prominent, high concentrations of retinol will irritate the skin, and its stability is poor, and its bioavailability will be limited. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and provide an anti-wrinkle composition and a preparation method and application thereof.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: an anti-wrinkle composition, comprising the following components in parts by weight: 0.1-1.5 parts of Mizuchiya polysaccharide, 0.1-70 parts of Haworthia extract, 10-230 parts of butylene glycol, and 50-600 parts of water.

[0006] In one embodiment, the mass of the Porphyra sutchuenensis polysaccharide is 0.05-0.5% of the mass of the anti-wrinkle composition.

[0007] In one embodiment, the mass ratio of the Porphyra aquatica polysaccharide to the Halocynthia sutchuenensis extract is 1:(1-9).

[0008] In one embodiment, the weight average molecular weight of the polysaccharide of the water-front temple laver is (1.2-2.0)×107 g / mol; and / or the weight average molecular weight of the seaweed extract is (4.5-5.5)×10 4 g / mol.

[0009] In one embodiment, based on 100% by weight of the anti-wrinkle composition, the mass percentage of the butylene glycol is less than 77%.

[0010] In one embodiment, the total sugar content of the anti-wrinkle composition is 0.5-0.7%.

[0011] In another aspect, the present disclosure provides use of the anti-wrinkle composition in preparing cosmetics.

[0012] In yet another aspect, the present disclosure provides a cosmetic comprising the anti-wrinkle composition.

[0013] In one embodiment, the weight percentage of the anti-wrinkle composition in the cosmetic is 0.5-10%.

[0014] In one embodiment, the cosmetic further comprises at least one auxiliary agent acceptable in the cosmetic field.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention uses Mizuzenji Porphyra polysaccharide and Halocynthia extract as active ingredients, and the two active ingredients are combined with each other to produce a sugar unit structure of N-acetylgalactosamine (GalNac) and galactose (α / βGal), and the produced sugar unit structure improves the moisturizing, hydrating, UV damage prevention and anti-wrinkle effects of the anti-wrinkle composition. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The state diagram of the anti-wrinkle composition of comparative example 2 after being placed under different conditions for different time periods;

[0017] Figure 2 The figure is a graph showing the inhibitory effect of the anti-wrinkle composition of Comparative Examples 3-5 on different lectins;

[0018] Figure 3 The graph is a graph showing the inhibitory effect of the anti-wrinkle composition of Comparative Examples 6-8 on different lectins;

[0019] Figure 4 Figure 1 is a graph showing the inhibitory effect of the anti-wrinkle composition of Examples 15-17 on different lectins;

[0020] Figure 5 The immunoblotting results of different concentrations of anti-wrinkle compositions on UVB-induced HS68 cells;

[0021] Figure 6 This is a graph showing the change in wrinkle depth after one of the volunteers used the eye cream in Application Example 2;

[0022] Figure 7 This is a picture of the changes in crow's feet after one of the volunteers used the eye cream in Application Example 2;

[0023] Figure 8 This is a picture of the changes in crow's feet of one of the volunteers before and after using the cream in Application Example 3;

[0024] Fig. 9 This is a picture of the changes in crow's feet of one of the volunteers before and after using the cream in Application Example 3;

[0025] Fig.10 This is a picture of the changes in under-eye wrinkles of one of the volunteers before and after using the cream in Application Example 3;

[0026] Fig.11 This is a picture of the changes in under-eye wrinkles of one of the volunteers before and after using the cream of Application Example 3. DETAILED DESCRIPTION

[0027] With reference to the detailed description of the following embodiments, it will be easier to understand the advantages and features of the present disclosure and the methods for achieving the advantages and features. However, the present disclosure can be implemented in various other forms and should not be construed as being limited to the embodiments set forth herein. The embodiments disclosed herein are provided so that the disclosure of the present disclosure will be thorough and complete, and the scope of the present disclosure will be fully conveyed to those skilled in the art.

[0028] The terms used in the specification are intended to describe certain embodiments only and should by no means limit the present disclosure.Expressions in the singular include the meaning of the plural form unless clearly used otherwise.

[0029] Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as those commonly understood by ordinary technicians in the field to which the present disclosure belongs. Any term defined in a comprehensive dictionary should be interpreted as having the same meaning in the context of the relevant field, and, unless explicitly defined otherwise, should not be interpreted as having an idealistic or overly formalistic meaning.

[0030] Hereinafter, the anti-wrinkle composition according to the present disclosure and the cosmetics containing the anti-wrinkle composition for skin moisturizing, hydrating, anti-wrinkle and prevention of ultraviolet damage will be described in detail.

[0031] The term "skin wrinkles" used herein refers to fine lines formed on the skin due to weakening of the skin, which may be caused by genetic factors, a decrease in collagen and elastin present in the dermis of the skin, the external environment, and the like.

[0032] As used herein, the term "anti-wrinkle / anti-wrinkle" refers to inhibiting or suppressing the generation of wrinkles on the skin or reducing wrinkles that have already occurred.

[0033] The term "UV damage" used in this article refers to the skin being exposed to ultraviolet rays, which causes massive necrosis of dermal cells and metabolic disorders, making it impossible for impurities in the cells to be metabolized away, causing the skin to develop sun spots, loss of elasticity, premature aging, sensitivity, wrinkles and other symptoms.

[0034] The term "preventing UV damage" as used herein refers to preventing or inhibiting the occurrence of various skin symptoms caused by skin exposure to UV rays.

[0035] As used herein, the term "safe and effective amount" means an amount of a compound or composition that is sufficient to induce the positive benefits as described herein, but low enough to avoid any serious side effects in the judgment of the skilled artisan.

[0036] The present disclosure provides an anti-wrinkle composition, comprising the following components in parts by weight: 0.1-1.5 parts of Porphyra sutchuenensis polysaccharide, 0.2-70 parts of Haworthia lappa extract, 10-230 parts of butylene glycol, and 50-600 parts of water.

[0037] The anti-wrinkle composition disclosed in the present invention uses APHANOTHECE SACRUM polysaccharide and GLOIOPELTIS FURCATA extract as active ingredients. After APHANOTHECE SACRUM polysaccharide and GLOIOPELTIS FURCATA extract are combined with each other, the anti-wrinkle composition contains sugar unit structures such as mannose, glucose, galactose, N-acetylgalactosamine, fucose, rhamnose and xylitol. On the one hand, N-acetylgalactosamine and galactose attract and retain moisture in the skin, so that the skin remains moisturized and soft, thereby improving the moisturizing and skin barrier effects of the anti-wrinkle composition. In addition, N-acetylgalactosamine and galactose can also improve the soothing and anti-inflammatory effects of the anti-wrinkle composition. On the second hand, On the one hand, N-acetylgalactosamine can promote the shedding of dead skin cells and exfoliation, making the skin smooth and delicate, and improving the anti-wrinkle effect of the anti-wrinkle composition; on the third hand, the anti-wrinkle composition can synergistically enhance the Langerin lectin of Langerhans cells, regulate immune function, inhibit the production of reactive oxygen species (ROS) and matrix metalloproteinase (MMP1), enhance cell viability and proliferation, promote the expression of COL1A1 and COL1A2, and promote the production of hyaluronic acid synthase (HAS1), further improving the anti-wrinkle effect of the anti-wrinkle composition.

[0038] Illustratively, the weight proportion of Mizuzenji Porphyra polysaccharide can be 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, or 1.5, but is not limited to the listed values, and other values ​​within the scope of this article are equally applicable.

[0039] Illustratively, the weight parts of the sea urchin extract can be 0.2 parts, 1 parts, 5 parts, 10 parts, 13 parts, 17 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 33 parts, 35 parts, 37 parts, 40 parts, 42 parts, 45 parts, 47 parts, 50 parts, 53 parts, 55 parts, 58 parts, 60 parts, 62 parts, 65 parts, 67 parts, and 70 parts, but are not limited to the listed values, and other values ​​within the scope of this article are equally applicable.

[0040] Exemplarily, the weight parts of water can be 50 parts, 70 parts, 90 parts, 110 parts, 130 parts, 150 parts, 170 parts, 190 parts, 200 parts, 240 parts, 280 parts, 300 parts, 330 parts, 360 parts, 400 parts, 450 parts, 500 parts, 550 parts, 600 parts, but are not limited to the listed values, and other values ​​within the scope of this article are equally applicable.

[0041] Illustratively, the weight parts of butanediol can be 10 parts, 20 parts, 30 parts, 40 parts, 50 parts, 60 parts, 70 parts, 80 parts, 90 parts, 100 parts, 110 parts, 120 parts, 130 parts, 140 parts, 150 parts, 160 parts, 170 parts, 180 parts, 190 parts, 200 parts, 210 parts, 220 parts, 230 parts, but are not limited to the listed values, and other values ​​within the scope of this article are equally applicable.

[0042] In one embodiment, the mass of the Mizuzenji Porphyra polysaccharide is 0.05-0.5% of the mass of the anti-wrinkle composition, for example, it can be 0.05%, 0.08%, 0.1%, 0.12%, 0.15%, 0.18%, 0.2%, 0.23%, 0.25%, 0.27%, 0.3%, 0.33%, 0.35%, 0.38%, 0.4%, 0.42%, 0.45%, 0.47%, 0.5%, but is not limited to the listed values, and other values ​​within the scope of this article are also applicable.

[0043] The content of Mizuchiensis laver polysaccharide in the anti-wrinkle composition will not only affect the stability of the anti-wrinkle composition, but also affect the moisturizing, anti-wrinkle and ultraviolet damage prevention effects of the anti-wrinkle composition. If the content of Mizuchiensis laver polysaccharide is too little, the binding sites of Mizuchiensis laver polysaccharide and sea urchin extract are few, which reduces the moisturizing, anti-wrinkle and ultraviolet damage prevention effects of the anti-wrinkle composition; if the content of Mizuchiensis laver polysaccharide is too much, some insoluble matter will appear in the anti-wrinkle composition, resulting in reduced stability, moisturizing, anti-wrinkle and ultraviolet damage prevention effects of the anti-wrinkle composition; when the mass of Mizuchiensis laver polysaccharide is 0.05-0.5% of the mass of the anti-wrinkle composition, the stability, moisturizing, anti-wrinkle and ultraviolet damage prevention effects of the anti-wrinkle composition are high.

[0044] In one embodiment, the mass ratio of the Mizuzenji Porphyra polysaccharide and the Halobium extract is 1:(1-9), for example, it can be 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5, 5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, but is not limited to the listed values, and other values ​​within the scope of this article are also applicable.

[0045] When the mass ratio of Mizuzenji Porphyra polysaccharide and the seaweed extract is within the range of 1:(1-9), the moisturizing and anti-wrinkle effects of the obtained anti-wrinkle composition are increased by 5-10%, compared with the mass ratio of Mizuzenji Porphyra polysaccharide and the seaweed extract outside the range of 1:(1-9).

[0046] In one embodiment, the weight average molecular weight of the Mizuzenji Porphyra polysaccharide is (1.2-2.0)×107 g / mol; for example, it can be 1.2×107 g / mol, 1.4×107 g / mol, 1.6×107 g / mol, 1.8×107 g / mol, 2.0×107 g / mol, but is not limited to the listed values, and other values ​​within the scope of this article are also applicable.

[0047] In one embodiment, the weight average molecular weight of the seaweed extract is (4.5-5.5)×10 4 g / mol, for example, it can be but not limited to 4.5×10 4 g / mol, 4.7×10 4 g / mol, 4.9×10 4 g / mol, 5.1×10 4 g / mol, 5.3×10 4 g / mol, 5.5×10 4 g / mol.

[0048] In one embodiment, based on 100% by weight of the anti-wrinkle composition, the weight percent of butylene glycol is less than 77%, preferably less than 65%, further preferably less than 55%, and more preferably 25-30%.

[0049] The mass percentage of butanediol in the anti-wrinkle composition affects the stability and antiseptic effect of the anti-wrinkle composition. The anti-wrinkle composition disclosed herein does not contain a preservative. Butanediol can be used as a preservative on the one hand, and can also be used as a solvent for the anti-wrinkle composition on the other hand. If the mass percentage of butanediol is too low, the antiseptic effect of the anti-wrinkle composition will be reduced, that is, the service life of the anti-wrinkle composition will be greatly shortened; if the mass percentage of butanediol is too high, the polysaccharide in the anti-wrinkle composition will precipitate, thereby reducing the stability of the anti-wrinkle composition; the inventors have found through research that when the mass percentage of butanediol is within a preferred range, an anti-wrinkle composition with good stability and antiseptic effect can be obtained.

[0050] In one embodiment, the total sugar content of the anti-wrinkle composition is 0.5-0.7%, for example, it can be 0.5%, 0.52%, 0.55%, 0.57%, 0.6%, 0.63%, 0.65%, 0.68%, 0.7%, but is not limited to the listed values, and other values ​​within the scope of this article are also applicable.

[0051] In one embodiment, the average molecular weight of the polysaccharide in the anti-wrinkle composition is 7.8-8.2 MDa.

[0052] The anti-wrinkle composition of the present invention has a high molecular weight, and the high molecular weight anti-wrinkle composition has good moisturizing, anti-wrinkle and UV damage prevention effects. Those skilled in the art know that high molecular weight substances are difficult to dissolve, while the anti-wrinkle composition of the present invention contains a wide variety of sugar unit structures. Due to the heterogeneity of the various sugar unit structures, the anti-wrinkle composition has high solubility.

[0053] The present disclosure also relates to the extraction steps of the polysaccharide of Mizuzenji Porphyra and the extract of Haworthia lappa.

[0054] In one embodiment, plant components (plant materials or plant ingredients (plant funish)) such as stems, fragments, leaves, fine powders, dust and / or coarse particles (shorts) are initially mixed with a solvent (e.g., water and / or other compounds) at an elevated temperature. For example, a variety of water-miscible solvents (e.g., alcohols, such as ethanol) can be combined with water to form an aqueous solvent. In some cases, the water content of the aqueous solvent can be greater than 50% by weight of the solvent. In one embodiment, the water content is at least about 70% by weight of the solvent, or at least about 80% by weight, or at least about 90% or about 100%. Deionized water, distilled water or tap water can be used. The amount of solvent in the suspension can vary widely, but the usual addition is about 75% to about 99% by weight of the suspension. However, the amount of solvent can vary with the nature of the solvent, the temperature at which the extraction is performed, and the type of plant components.

[0055] After forming the solvent / plant ingredient mixture, some or all of the soluble extract fraction of the ingredient mixture can be optionally separated (e.g., extracted) from the mixture. If desired, the aqueous solvent / plant ingredient mixture can be agitated during the extraction process by stirring, vibrating, or otherwise mixing the mixture to increase the extraction rate. Typically, the extraction is performed for about 0.5 hours to about 6 hours.

[0056] Prior to the extraction step, an optional grinding or cutting step may be used to break up the plant or plant parts and thus disrupt the cell walls of the plant. Once separated from the insoluble residue fraction of the plant solution, the soluble extract fraction may optionally be concentrated using any known type of concentrator (e.g., vacuum evaporator). In one embodiment, the soluble components may be highly concentrated. In addition, the concentrated or unconcentrated soluble extract fraction may be used in any desired manner.

[0057] In one embodiment, extraction can also be performed by a method other than using hot water, i.e., by extracting with a supercritical gas (e.g., carbon dioxide) or by using, for example, ethanol, hexane, acetone, R134a (1,1,1,2-tetrafluoroethane), carbon dioxide, and hydrofluorocarbons. In one embodiment, extraction can be performed by using at least one solvent at room temperature and atmospheric pressure. Extraction can also be performed using a mixture of different solvents. In another embodiment, extraction can be performed using at least one solvent (e.g., R134a or carbon dioxide) at different temperatures and different pressures and in different states (liquid or gaseous). For example, extraction can be performed using a solvent in a liquid state (e.g., a solvent that is volatile or non-volatile at room temperature), a subcritical state (e.g., water at a temperature higher than 100° C. and a pressure higher than 1 bar), or a supercritical state (e.g., carbon dioxide at a temperature higher than 31° C. and a pressure higher than 73 bar).

[0058] In conjunction with the present disclosure, processing aids may be used to improve extraction, such as pH adjusters (e.g., NaOH or organic acids), microwaves, pressure, ultrasound, enzymes (e.g., proteases, amylases, celluloses and / or pectinases). When "extraction" is mentioned herein, the term includes the aforementioned alternative extraction means. The extraction used in conjunction with the present disclosure may be performed in a continuous or discontinuous manner.

[0059] In one embodiment, at least a portion of fresh, frozen or dried plant material may be used for extraction and / or pressing, the portion selected from one or more of roots, stems, trunks, main stems, leaves, blades, fruits, flowers, seeds or bark.

[0060] The soluble part (plant extract) and the insoluble part (solid plant particles) can be separated by separating the liquid and solid phases, for example by filtration (with or without pressure), by centrifugation or other methods commonly used in the laboratory and known to the skilled person.

[0061] Those skilled in the art can use means known in the art to evenly mix the polysaccharide of Porphyra sutchuenensis, the extract of Haworthia sutchuenensis, butylene glycol and water to obtain an anti-wrinkle composition, such as mechanical stirring, homogenization, or ultrasound, etc. After mixing, a uniform and stable anti-wrinkle composition is obtained, and the active ingredients are fully mixed, which is conducive to the full release of the active ingredients.

[0062] In one embodiment, the preparation method of the Shuiqiansi Porphyra polysaccharide is:

[0063] (1) removing the water-soluble pigment of the polysaccharide of the water-front temple laver by freezing and thawing, and then removing the fat-soluble pigment of the polysaccharide of the water-front temple laver by hypochlorous acid or activated carbon to obtain a decolorized solid;

[0064] (2) dissolving the decolorized solid obtained in step (1) with a sodium hydroxide solution, then adjusting the pH, filtering, washing, and drying to obtain the Mizuzenji Porphyra polysaccharide.

[0065] Specifically, the freezing and thawing are performed 2-5 times.

[0066] Specifically, the mass percentage of sodium hydroxide in the sodium hydroxide aqueous solution is 10%-18%.

[0067] Specifically, the end point of pH adjustment is to adjust pH to 6.0-7.0.

[0068] Specifically, the washing solvents are water and isopropanol.

[0069] Specifically, there is no specific method for drying, and it can be, for example, air drying, oven drying, freeze drying, etc.

[0070] In one embodiment, the preparation method of the extract of Haworthia lappa is:

[0071] After grinding and defatting the seaweed at room temperature, the obtained first product is stirred with water, and then filtered, washed and dried to obtain the seaweed extract.

[0072] Specifically, before grinding and defatting, the seaweed needs to be pre-treated, and the pre-treatment is washing and drying. The pre-treatment can remove impurities such as sand, dust, etc. on the seaweed.

[0073] Specifically, the defatting step is: placing the crushed seaweed powder in ethanol for reflux extraction for 2-3 hours to remove the fat components in the algae; the defatted seaweed powder is centrifuged and dried at 50-65°C to constant weight.

[0074] Specifically, the mass ratio of the first product to water is 1:5-20.

[0075] Specifically, the washing solvents are water and isopropanol.

[0076] In a second aspect, the present disclosure provides use of the anti-wrinkle composition in preparing cosmetics.

[0077] In a third aspect, the present disclosure provides a cosmetic comprising the anti-wrinkle composition.

[0078] In one embodiment, the weight percentage of the anti-wrinkle composition in the cosmetic is 0.5-10%, for example, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, but is not limited to the listed values, and other values ​​within the scope of this document are also applicable.

[0079] Adding a specific proportion of the anti-wrinkle composition to cosmetics can bring good anti-wrinkle effects to the cosmetics. When the cosmetics of the present application contain less than 0.5% by weight of the anti-wrinkle composition of the present application, it is not possible to expect sufficient effects of enhancing skin elasticity or improving wrinkles; or moisturizing the skin, and when it contains more than 10% by weight, allergic reactions or skin safety problems may occur.

[0080] In one embodiment, the cosmetics may be in the form of a general emulsified dosage form and a soluble dosage form. For example, the cosmetics may have a toner such as a skin softener or a nutrient toner, an emulsion such as a facial lotion or a body lotion, a cream such as a nutrient cream, a moisturizing cream, an eye cream, an essence, a cosmetic ointment, a balm, a spray, a gel, a mask, a sunscreen, a primer, a liquid, solid or spray-type foundation, a powder, a makeup remover such as a cleansing cream, a cleansing lotion, a cleansing oil, a cleansing foam, a soap, a shower gel, and the like.

[0081] The cosmetic may further comprise at least one cosmetically acceptable auxiliary; for example, fatty substances, moisturizers, emollients, conditioners, emollients, thickeners, organic solvents, suspending agents, solubilizers, concentrates and gelling agents, antioxidants, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion blocking agents and chelating agents, preservatives, vitamins, blocking agents, moisturizers, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics.

[0082] Each of the above-mentioned ingredients contained in the composition according to the present application may preferably be contained in the composition according to the present application within a range not exceeding the maximum usage amount indicated in the specifications stipulated by various governments.

[0083] In one embodiment, the cosmetic is an eye cream, and the eye cream comprises the following components in weight percentage: 0.5-10% anti-wrinkle composition, 0.3-1% parahydroxyacetophenone, 1-6% butylene glycol, 0.05-0.3% xanthan gum, 0.5-1.5% emulsifier, 0.5-10% polydimethylsiloxane, 0.5-10% caprylic / capric triglyceride, 0.3-1% 1,2-hexanediol, and the balance deionized water; wherein the emulsifier is composed of sodium polyacrylate, ethylhexyl stearate, and trideceth-6 in a mass ratio of 62:34:4.

[0084] In one embodiment, the cosmetic is a gel, and the gel comprises the following components in weight percentage: 0.5-10% of an anti-wrinkle composition, 0.3-1.0% of parahydroxyacetophenone, 0.5-1.5% of an emulsifier, 0.3-1.0% of 1,2-hexanediol, and the balance of deionized water; wherein the emulsifier is composed of sodium polyacrylate, ethylhexyl stearate, and trideceth-6 in a mass ratio of 62:34:4.

[0085] In one embodiment, the cosmetic is a facial cream, and the facial cream comprises the following components in weight percentage: 0.5-10% of an anti-wrinkle composition, 0.5-1% of parahydroxyacetophenone, 0.5-5% of butylene glycol, 0.05-0.2% of xanthan gum, 0.02-0.5% of sodium stearoyl glutamate, 0.3-1.0% of ammonium acryloyldimethyltaurate / VP copolymer, 1.0-2.5% of a mixture of cetearyl olive oil ester and sorbitan olive oil ester, 0.5-3.0% of cetearyl alcohol, 0.5-5.0% of caprylic / capric triglyceride, 0.5-5.0% of hydrogenated polydecene, 0.5-5.0% of polydimethylsiloxane with a kinematic viscosity of 100 cSt, 0.5-5.0% of polydimethylsiloxane with a kinematic viscosity of 5 cSt, LIMNANTHES ALBA) seed oil 0.5-5.0%, 1,2-hexanediol 0.5-1.0%, and deionized water the rest; wherein the mass ratio of cetearyl olive oil ester to sorbitan olive oil ester is 5-7:3-5.

[0086] The cosmetic "includes" means that in addition to the raw materials, it can also include other raw materials, which give the cosmetic different characteristics. In addition, the "includes" described in the present disclosure can also be replaced by the closed "is" or "consisting of..."

[0087] It should be noted that the balance is water, which means that the eye cream raw materials of the present application exclude the anti-wrinkle composition, p-hydroxyacetophenone, butylene glycol, xanthan gum, emulsifier, polydimethylsiloxane, caprylic / capric triglyceride, 1,2-hexanediol, and optionally other components, and the balance is water; the sum of the weight percentages of the anti-wrinkle composition, p-hydroxyacetophenone, butylene glycol, xanthan gum, emulsifier, polydimethylsiloxane, caprylic / capric triglyceride, 1,2-hexanediol, and optionally other components is 100%;

[0088] Or it means that the anti-wrinkle composition, p-hydroxyacetophenone, emulsifier, 1,2-hexanediol, and optionally other components are removed from the gel raw materials of the present application, and the balance is water; the sum of the weight percentages of the anti-wrinkle composition, p-hydroxyacetophenone, emulsifier, 1,2-hexanediol, and optionally other components is 100%;

[0089] Or it refers to the raw materials of the facial cream of the present application except the anti-wrinkle composition, p-hydroxyacetophenone, butylene glycol, xanthan gum, sodium stearoyl glutamate, ammonium acryloyldimethyltaurate / VP copolymer, a mixture of cetearyl olive oil ester and sorbitan olive oil ester, cetearyl alcohol, caprylic / capric triglyceride, hydrogenated polydecene, polydimethylsiloxane with a kinematic viscosity of 100 cSt, polydimethylsiloxane with a kinematic viscosity of 5 cSt, LIMNANTHES ALBA seed oil, 1,2-hexanediol and optionally other components, and the balance is water.

[0090] Another aspect of the present disclosure also relates to a method for preparing the gel, comprising the following steps: adding deionized water, parahydroxyacetophenone and an emulsifier into a container, heating to 75-80° C. and mixing evenly, homogenizing at a speed of 8000-100000 rpm for 2-3 minutes, then cooling to 45° C., adding an anti-wrinkle composition and 1,2-hexanediol in sequence, and stirring evenly.

[0091] Another aspect of the present disclosure also relates to a method for preparing the eye cream comprising the following steps:

[0092] (1) adding deionized water, p-hydroxyacetophenone, butylene glycol and xanthan gum into a container, heating to 75-80° C. and mixing evenly to obtain phase A, and keeping the temperature for later use;

[0093] (2) Add polydimethylsiloxane and caprylic / capric triglyceride into a container, heat to 75-80° C. and mix well to obtain phase B, which is kept warm for later use;

[0094] (3) Add the phase B obtained in step (2) and the emulsifier to the phase A obtained in step (1), homogenize at a speed of 8000-100000 rpm for 2-3 minutes, then cool to 45° C., add the anti-wrinkle composition and 1,2-hexanediol in sequence, and stir evenly.

[0095] Another aspect of the present disclosure also relates to a method for preparing the facial cream comprising the following steps:

[0096] (1) adding deionized water, p-hydroxyacetophenone, butylene glycol, xanthan gum and sodium stearoyl glutamate into a container, heating to 80-85° C. and mixing evenly to obtain phase A, and keeping the temperature for later use;

[0097] (2) adding a mixture of cetearyl olivate and sorbitan olivate, cetearyl alcohol, caprylic / capric triglyceride, hydrogenated polydecene, polydimethylsiloxane with a kinematic viscosity of 100 cSt, polydimethylsiloxane with a kinematic viscosity of 5 cSt, and Limnanthes alba seed oil into a container, heating to 80-85° C. and mixing evenly, adding ammonium acryloyldimethyltaurate / VP copolymer, mixing evenly, obtaining phase B, and keeping warm for later use;

[0098] (3) Add the phase B obtained in step (2) to the phase A obtained in step (1), homogenize at a speed of 8000-100000 rpm for 2-3 minutes, then cool to 45° C., add the anti-wrinkle composition and 1,2-hexanediol in sequence, and stir evenly.

[0099] In order to further understand the present application, the anti-wrinkle composition of the present application, its preparation method and effects are further described in detail below in conjunction with specific examples.

[0100] Shuiqiansi laver polysaccharide is homemade, and its preparation method is as follows:

[0101] (1) removing the water-soluble pigment of the polysaccharide of the water-front temple laver by freezing and thawing, and then removing the fat-soluble pigment of the polysaccharide of the water-front temple laver by hypochlorous acid to obtain a decolorized solid;

[0102] (2) dissolving the decolorized solid obtained in step (1) with a sodium hydroxide solution having a mass percentage content of 10%, wherein the mass ratio of the sodium hydroxide solution to the decolorized solid is 20:1, and then adjusting the pH to pH = 6.0-6.5, filtering, and washing the obtained filtrate with water for 3 times, and drying to obtain the polysaccharide of Mizukienji Porphyra, wherein the molecular weight of the polysaccharide of Mizukienji Porphyra is 1.6×107 g / mol.

[0103] The extract of the seaweed was prepared in-house. The preparation method thereof was as follows: fresh seaweed was washed and dried, then ground and defatted at room temperature to obtain a first product, the first product and water were mixed evenly at a mass ratio of 1:10, stirred at room temperature for 24 hours, filtered, the obtained filtrate was washed with water three times, and dried to obtain the seaweed extract. The weight average molecular weight of the seaweed extract was 4.9×10 4 g / mol.

[0104] Examples and Comparative Examples

[0105] The components and weight portions of the anti-wrinkle composition of the embodiment and comparative example are shown in Table 1. The preparation method of the anti-wrinkle composition of the embodiment and comparative example comprises the following steps: starting stirring, adding each component according to the weight portion in Table 1 into a container at a rotation speed of 800 rpm, stirring for 30 minutes, and obtaining the anti-wrinkle composition.

[0106] Table 1

[0107]

[0108]

[0109] Test Example 1

[0110] This test example tests the stability of the anti-wrinkle compositions obtained in various examples and comparative examples.

[0111] Test method: The anti-wrinkle compositions obtained in the examples and comparative examples were placed under -18°C, 5°C, RT, 40°C, 50°C, UV and -10 / 45°C cycle conditions, and taken out at different time points to restore to room temperature, and then the sensory properties were evaluated and the physicochemical properties were measured. Judgment rules: ○: normal; ×: abnormal; +: degree of discoloration.

[0112] Overall determination requirements: 1. UV requirement D 14 Stable, less than 14 days is considered unqualified; 2. Temperature stability requirement D 1M Stable, below D 1M Judged as unqualified.

[0113] The color test results of the anti-wrinkle composition at 50° C. and UV conditions are shown in Table 2.

[0114] Table 2

[0115]

[0116]

[0117]

[0118] After testing, it can be known that the appearance of Examples 1-17 and Comparative Examples 3-8 is a transparent and / or translucent liquid. After being placed under the cycle conditions of -18°C, 5°C, RT, 40°C, 50°C, UV and -10 / 45°C for 3 months, the appearance of Examples 1-17 and Comparative Examples 3-8 did not change significantly; the appearance of Comparative Example 1 is a liquid containing flocs. After being placed under the cycle conditions of -18°C, 5°C, RT, 40°C, 50°C, UV and -10 / 45°C for 3 months, the flocs increased significantly; the appearance of Comparative Example 2 is a liquid containing insoluble matter. After being placed under the cycle conditions of -18°C, 5°C, RT, 40°C, 50°C, UV and -10 / 45°C for 3 months, the appearance of Comparative Example 2 did not change significantly.

[0119] After the anti-wrinkle compositions of Examples 1-17 and Comparative Examples 1-8 were placed under -18°C, 5°C, RT, 40°C, 50°C, UV and -10 / 45°C cycle conditions for 3 months, there was no significant change in odor.

[0120] After being placed under -18°C, 5°C, RT, 40°C and -10 / 45°C cyclic conditions for 3 months, the anti-wrinkle compositions of Examples 1-17 and Comparative Examples 1-8 showed no obvious color change; when placed under 50°C and UV conditions, the color changes of the anti-wrinkle compositions of Examples 1-17 and Comparative Examples 1-8 at different time points are shown in Table 2. As can be seen from Table 2, the color changes of the anti-wrinkle compositions of the present invention all meet the stability requirements, while the content of butanediol in Comparative Example 1 is too much, and after being placed under UV conditions for 2 months, it begins to turn brown, indicating that the anti-wrinkle composition of Comparative Example 2 has poor stability; the content of Mizukienji Porphyra polysaccharide in Comparative Example 2 is too much, and after being placed under UV conditions for 7 days, it begins to turn brown, indicating that the anti-wrinkle composition of Comparative Example 2 has poor stability.

[0121] Figure 1The state diagram of the anti-wrinkle composition of comparative example 2 after being placed under different conditions for different time periods, from Figure 1 It can be seen that the anti-wrinkle composition of Comparative Example 2 begins to turn brown after being placed under UV conditions for 7 days, and the color further deepens as the placement time increases; after being placed under 50° C. conditions for 1 month, the color turns yellow.

[0122] Test Example 2

[0123] This test example tests the sugar unit structures contained in the anti-wrinkle compositions of Examples 15-17 and Comparative Examples 3-8.

[0124] This test example determines the interaction of the anti-wrinkle compositions of Examples 15-17 and Comparative Examples 3-8 with 25 lectins, thereby identifying the sugar unit structure contained in the anti-wrinkle compositions. By obtaining sugar spectra from different samples, the ability of the samples to be recognized by lectins can be evaluated. The results are shown in FIG. Figure 2-4 And as shown in Table 3.

[0125] Table 3 Inhibition rate of each lectin by the anti-wrinkle composition of Examples 15-17 and Comparative Examples 3-8 (in %)

[0126]

[0127]

[0128] Note: Fuc: fucose, Glc: glucose, Gal: galactose, Man: mannose, GalNAc: n-acetylgalactosamine, GlcNAc: n-acetylglucosamine, GlcU: glucuronic acid, NeuAc: neuraminic acid, Rha: rhamnose, Xyl: xylitol, βglucane: β-glucan.

[0129] Figure 2 The inhibitory effects of the anti-wrinkle compositions of comparative examples 3-5 on different lectins are shown in FIG. Figure 3 The inhibitory effects of the anti-wrinkle compositions of Comparative Examples 6-8 on different lectins are shown in FIG. Figure 4 The inhibitory effects of the anti-wrinkle compositions of Examples 15-17 on different lectins. Figure 2-4 From the experimental data in Table 3, it can be seen that the anti-wrinkle composition containing only Mizukiensis polysaccharide or Henia algae extract has no or almost no inhibitory effect on DBA and AIA at low concentrations, while the anti-wrinkle composition containing both Mizukiensis polysaccharide and Henia algae extract can synergistically enhance the effect of the anti-wrinkle composition on lectins, such as DBA, AIA, Langerin, Gal3, etc.; This indicates that the Mizukiensis polysaccharide and Henia algae extract in the anti-wrinkle composition of the present application have a synergistic effect.

[0130] Test Example 3

[0131] This test example tests the effect of the anti-wrinkle composition of Example 16 in preventing ultraviolet damage.

[0132] 1. Materials

[0133] HS68 (CRL-1635) human dermal fibroblasts were obtained from the American Type Culture Collection (ATCC); DMEM medium, penicillin, streptomycin, cinnamyl alcohol, HEPES, phosphatase inhibitor cocktail, and Triton X-100 were purchased from Nacalai Tesque (Kyoto, Japan); ethylenediaminetetraacetic acid (EDTA), skim milk powder, Tween 20, and dithiothreitol (DTT) were purchased from Wako Pure Chemical (Osaka, Japan); IGF-1 was purchased from Abcam (Tokyo, Japan); Hybond-ECL membrane was obtained from GE Healthcare UK, Ltd (Buckinghamshire, UK), and protease inhibitor cocktail was obtained from Roche Applied Science (Mannheim, Germany); fetal bovine serum (FBS) was purchased from Invitrogen (Carlsbad, CA, USA); and the EZ-ECL chemiluminescent detection kit for chemiluminescent detection was obtained from Israel Bioindustry (Kibbutz Beit Haemek, Israel), Example 16 Anti-wrinkle composition.

[0134] 2. Cell culture

[0135] HS68 (CRL-1635) human dermal fibroblasts were cultured in DMEM medium containing 10% (V / V) FBS, 100 U / mL penicillin, and 100 μg / mL streptomycin. The cells were incubated in 5% CO 2 , cultured at 37°C.

[0136] 3. Experimental methods

[0137] 3.1 Toxicity of the anti-wrinkle composition to HS68 cells

[0138] A normal control group and different doses of irradiation groups (the irradiation group was further divided into groups irradiated with 0.01, 0.1, 1, 5, and 10 μg / mL of the anti-wrinkle composition, respectively) were set up in a 96-well plate. HS68 cells in the logarithmic growth phase were taken and cultured at 1×10 4 The cells were plated in a 96-well plate at a density of 100 μg / well. Each group had 6 experimental wells. The edge wells were filled with sterile PBS and incubated in 5% CO 2, cultured at 37°C for 24h, and when the cells are completely attached, the above-mentioned anti-wrinkle composition is added to the irradiation group of the 96-well plate at 50μL per well, and cultured for 24h, the culture medium is discarded, and an equal amount of growth medium and 20μL MTT solution (5mg / mL) are added to each well and cultured for 4h, the culture medium containing MTT is discarded, and 200μL DMSO is added to each well to dissolve the crystallized product, and the absorbance value is measured at a wavelength of 570nm using an enzyme marker. Cell survival rate (%) = absorbance value of irradiation group / absorbance value of control group × 100%.

[0139] 3.2 Effects of anti-wrinkle composition on UVB-induced HS68 cells

[0140] A negative control group (not treated with the anti-wrinkle composition and UVB) and different doses of irradiation groups (the irradiation group was further divided into groups irradiated with 0, 0.01, 0.1, 1, 5, and 10 μg / mL of the anti-wrinkle composition) were set up in a 96-well plate. HS68 cells in the logarithmic growth phase were taken and cultured at 1×10 4 The cells were plated in a 96-well plate at a density of 100 μg / well. Each group had 6 experimental wells. The edge wells were filled with sterile PBS and incubated in 5% CO 2 The cells were cultured at 37°C for 24 h. When the cells were completely attached to the wall, 50 μL of the above-mentioned anti-wrinkle composition was added to the irradiation group of the 96-well plate at each well. After culturing for 24 h, the culture medium was discarded, and the cells were washed with PBS. The cells were treated with UVB using an ultraviolet crosslinker (UVP, Upland, CA, USA) at a dose of 30 mJ / cm 2 After the treatment, the MTT test was performed. The specific method of the MTT test was the same as that in "3.1".

[0141] 3.3DCF fluorescence measurement of ROS levels in HS68 cells

[0142] After inoculation and treatment of HS68 cells according to the "3.2" method, they were stained with 10 μM DCFH-DA and analyzed using an Axiovert200M fluorescence spectrophotometer (Zeiss, Oberkochen, Germany). The level of intercellular ROS was displayed by DCFH-DA fluorescence and measured on a microplate reader (Infinite M200 Pro, Tecan, Switzerland) with an excitation wavelength of 485 nm and an emission wavelength of 530 nm.

[0143] 3.4 SDS-PAGE and immunoblotting analysis

[0144] After inoculating and treating HS68 cells according to the method of "3.2", take the cell suspension of each group, centrifuge to remove the supernatant, and then add 100 μL cell lysis solution (protease inhibitor: 1×RIPA buffer (10mM Tris·HCl, pH 7.4, 150mMNaCl, 1% sodium deoxycholate, 1% Triton X-100, 0.1% SDS and 1mM EDTA)) to lyse the cells, and then sonicate for 10 seconds. The protein concentration of each lysate was determined using BCA protein assay reagent.

[0145] After reduction with 50mM DTT, the protein was run on an 8% SDS-polyacrylamide gel, transferred to a nitrocellulose membrane, incubated with 1×TBST buffer solution containing 5% skim milk at room temperature for 1h, and washed 3 times in 1×TBST buffer solution for 10 minutes. The membrane where the target protein is located was added with the corresponding primary antibody solution and incubated overnight on a shaker at 4°C. After overnight, it was washed 3 times with 1×TBST at room temperature for 20-30min each time, and the corresponding secondary antibody solution was added, incubated on a shaker at room temperature for 2h in a dark place, and then washed 3 times with 1×TBST at room temperature for 30min each time. Incubated with horseradish peroxidase (HRP)-conjugated secondary antibody at room temperature for 1 hour.

[0146] Antibodies against MMP1, HAS1, COL1A1, COL1A2 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Cellsignaling Technologies (Danver, USA). The protein levels of MMP-1, HAS1, COLa1 and COLa2 were determined by scanning the membrane using a chemiluminescent imaging system (Bio-Rad).

[0147] 3.5 Real-time qPCR quantification

[0148] A negative control group (not treated with the anti-wrinkle composition and UVB) and different doses of irradiation groups (the irradiation group was further divided into 0, 0.01, 0.1, 1, and 5 μg / mL anti-wrinkle composition intervention groups) were set up in a 6-well plate. HS68 cells in the logarithmic growth phase were taken and incubated in 5% CO 2 The cells were cultured at 37°C for 24 h. When the cells were completely attached to the wall, 50 μL of the above-mentioned anti-wrinkle composition was added to the irradiation group of the 6-well plate at each well. After culturing for 24 h, the culture medium was discarded, and the cells were washed with PBS. The cells were treated with UVB using an ultraviolet crosslinker (UVP, Upland, CA, USA) at a dose of 30 mJ / cm 2At the end of treatment, total RNA was collected using the RNeasy Mini RNA isolation kit (Qiagen, Valencia, CA, USA). RNA was eluted into RNase-free water, quantified by UV absorbance, and stored at −80°C until use. Reverse transcription (RT)-PCR was performed on an Applied Biosystems 7500 Real-Time PCR system using the following amplification conditions: preincubation at 95°C for 10 min, 40 cycles at 95°C for 10 s, annealing at 60°C for 15 s, and extension at 72°C for 15 s. Gene expression levels were normalized to GAPDH and calculated using the comparative ΔΔCT method.

[0149] 3.6 Statistical methods

[0150] All experiments were performed in parallel for 3 times, and the results are expressed as mean ± standard deviation (SD). SPSS software (SPSS, Inc., Tokyo, Japan) was used for statistical analysis. Student's t-test, analysis of variance, and appropriate post hoc tests (Tukey's) were performed according to the nature and distribution of the data. p < 0.05 was considered statistically significant. The results are shown in Tables 4-7 and Figure 5 shown.

[0151] 4. Results and Analysis

[0152] 4.1 Toxicity of the anti-wrinkle composition on HS68 cells

[0153] Table 4 Toxicity effects of different concentrations of anti-wrinkle compositions on HS68 cells

[0154]

[0155] As can be seen from Table 4, different concentrations of anti-wrinkle composition intervention had no obvious toxic effect on cells after 24 hours. Compared with the normal control group, with the increase of the concentration of anti-wrinkle composition, the cell survival rate did not change significantly, and the difference was not obvious and statistically significant (P>0.05). Therefore, different concentrations of anti-wrinkle composition pretreatment had no obvious cytotoxic effect on HS68 cell viability, and had little effect on HS68 cell survival rate.

[0156] 4.2 Effect of anti-wrinkle composition on UVB-induced HS68 cell survival

[0157] Table 5 Effects of different concentrations of anti-wrinkle compositions on the survival of HS68 cells induced by UVB

[0158]

[0159] As can be seen from Table 5, compared with the negative control group, the survival rate of HS68 cells after UVB irradiation was 73.43%. When the concentration of the anti-wrinkle composition was in the range of 0.01-0.1 μg / mL, the protective effect on HS68 cells increased in a dose-dependent manner. When the concentration of the anti-wrinkle composition was 0.1-5 μg / mL, it had a better proliferation effect on HS68 cells, which was significantly different from the group not treated with the anti-wrinkle composition (P<0.01).

[0160] 4.3 Effects of anti-wrinkle composition on ROS levels in UVB-induced HS68 cells

[0161] Table 6 Effects of different concentrations of anti-wrinkle compositions on ROS levels in HS68 cells induced by UVB

[0162]

[0163] As shown in Table 6, UVB irradiation increased the ROS content in HS68 cells to 175.9%. As the concentration of the anti-wrinkle composition increased, the anti-wrinkle composition gradually enhanced its scavenging effect on ROS produced by UVB damage, and it was dose-dependent. Among them, when 0.01-5μg / mL of the anti-wrinkle composition acted on HS68 cells irradiated by UVB, the ROS in the cells decreased significantly compared with those not treated with the anti-wrinkle composition, and the decrease increased with the increase of the dose (P<0.01).

[0164] 4.4 Effect of anti-wrinkle composition on UVB-induced expression of related proteins in HS68 cells

[0165] Table 7 Effects of different concentrations of anti-wrinkle compositions on the expression of related proteins in HS68 cells induced by UVB

[0166]

[0167] (1) The anti-wrinkle composition inhibits UVB-induced MMP1 production in HS68 cells

[0168] The experimental results are shown in Table 7. UVB irradiation promotes DNA damage of skin cells by generating oxidative stress and ROS, leading to further structural and functional damage. The increased expression of MMP1 caused by UVB irradiation is one of the key factors leading to ECM protein degradation. 0.01-5 μg / mL anti-wrinkle composition can reduce the expression of MMP1 in a dose-dependent manner. Compared with the irradiation group without anti-wrinkle composition intervention, even with the lowest dose of 0.01 μg / mL anti-wrinkle composition intervention, the reduction in MMP1 production was statistically significant (P<0.01).

[0169] (2) The anti-wrinkle composition promotes the production of HAS1 in HS68 cells induced by UVB

[0170] Hyaluronan synthase 1 (HAS1) is an enzyme that plays a major role in the production of hyaluronic acid in the skin; the experimental results are shown in Table 7. HAS1 in HS68 cells was significantly reduced after UVB irradiation, and the anti-wrinkle composition was able to increase UVB-induced HAS1 production. Compared with the irradiated group without anti-wrinkle composition intervention, the production of HAS1 was statistically significant after intervention with 0.01-5 μg / mL anti-wrinkle composition (P<0.01).

[0171] (2) The anti-wrinkle composition promotes the production of COL1A1 and COL1A2 in HS68 cells induced by UVB

[0172] Collagen is an important ECM protein, and type 1 collagen accounts for about 80% of dermal collagen. Type 1 collagen is composed of two α1(I) chains and one α2(I) chain, which are encoded by two genes, COL1A1 and COL1A2, respectively. The protein expression levels of COL1A1 and COL1A2 were evaluated by immunoblotting and Q-PCR. The experimental results are shown in Figure 5 As shown in Table 7, UVB irradiation reduced the production and expression of type 1 collagen subunits. Pretreatment with the anti-wrinkle composition effectively resisted this reduction at all tested concentrations. Compared with the irradiated group without anti-wrinkle composition intervention, the production of COL1A1 and COL1A2 was statistically significant after intervention with 0.01-5 μg / mL anti-wrinkle composition (P<0.01).

[0173] In summary, the anti-wrinkle composition disclosed herein can effectively prevent ultraviolet damage.

[0174] Application Example 1

[0175] This application example provides a gel comprising the following components in percentage by weight: 1% of the anti-wrinkle composition of Example 6, 0.5% of parahydroxyacetophenone, 0.8% of an emulsifier, 0.5% of 1,2-hexanediol, and the remainder of deionized water; wherein the emulsifier is composed of sodium polyacrylate, ethylhexyl stearate, and trideceth-6 in a mass ratio of 62:34:4.

[0176] This application example also provides a method for preparing the gel, comprising the following steps:

[0177] Deionized water, p-hydroxyacetophenone and emulsifier were added to a container, heated to 78°C and mixed evenly, homogenized at 9000 rpm for 3 minutes, then cooled to 45°C, and the anti-wrinkle composition of Example 6 and 1,2-hexanediol were added in sequence, and stirred evenly.

[0178] Application Example 2

[0179] This application example provides an eye cream, comprising the following components in weight percentage: 5% of the anti-wrinkle composition of Example 6, 0.5% of parahydroxyacetophenone, 3% of butylene glycol, 0.2% of xanthan gum, 1% of an emulsifier, 5% of polydimethylsiloxane, 3% of caprylic / capric triglyceride, 0.5% of 1,2-hexanediol, and the balance of deionized water; wherein the emulsifier is composed of sodium polyacrylate, ethylhexyl stearate, and trideceth-6 in a mass ratio of 62:34:4.

[0180] This application example also provides a method for preparing the eye cream, comprising the following steps:

[0181] (1) adding deionized water, p-hydroxyacetophenone, butylene glycol and xanthan gum into a container, heating to 78° C. and mixing evenly to obtain phase A, and keeping the temperature for later use;

[0182] (2) adding polydimethylsiloxane and caprylic / capric triglyceride into a container, heating to 78° C. and mixing evenly to obtain phase B, and keeping the mixture warm for later use;

[0183] (3) Add the phase B obtained in step (2) and the emulsifier to the phase A obtained in step (1), homogenize at a speed of 9000 rpm for 3 minutes, then cool to 45° C., add the anti-wrinkle composition of Example 6 and 1,2-hexanediol in sequence, and stir evenly.

[0184] Application Example 3

[0185] This application example provides a facial cream, comprising the following components in weight percentage: 1% anti-wrinkle composition, 0.5% parahydroxyacetophenone, 3% butylene glycol, 0.12% xanthan gum, 0.05% sodium stearoyl glutamate, 0.6% ammonium acryloyldimethyltaurate / VP copolymer, 1.8% mixture of cetearyl olivate and sorbitan olivate, 2% cetearyl alcohol, 4% caprylic / capric triglyceride, 2% hydrogenated polydecene, 2% polydimethylsiloxane with a kinematic viscosity of 100 cSt, 2% polydimethylsiloxane with a kinematic viscosity of 5 cSt, 2% LIMNANTHES ALBA seed oil, and 1% 1,2-hexanediol; wherein the mass ratio of cetearyl olivate to sorbitan olivate is 6:4.

[0186] This application example also provides a method for preparing the facial cream, comprising the following steps:

[0187] (1) adding deionized water, p-hydroxyacetophenone, butylene glycol, xanthan gum and sodium stearoyl glutamate into a container, heating to 82° C. and mixing evenly to obtain phase A, and keeping the temperature for later use;

[0188] (2) adding a mixture of cetearyl olivate and sorbitan olivate, cetearyl alcohol, caprylic / capric triglyceride, hydrogenated polydecene, polydimethylsiloxane with a kinematic viscosity of 100 cSt, polydimethylsiloxane with a kinematic viscosity of 5 cSt, and Limnanthes alba seed oil into a container, heating to 80° C. and mixing well, adding ammonium acryloyldimethyltaurate / VP copolymer, mixing well, obtaining phase B, and keeping warm for later use;

[0189] (3) Add the phase B obtained in step (2) and the emulsifier to the phase A obtained in step (1), homogenize at a speed of 9000 rpm for 3 minutes, then cool to 45° C., add the anti-wrinkle composition of Example 6 and 1,2-hexanediol in sequence, and stir evenly.

[0190] Comparative application example 1

[0191] This comparative application example provides a gel, which differs from Application Example 1 only in that the anti-wrinkle composition of Example 6 is replaced by deionized water, that is, this comparative application example does not contain the anti-wrinkle composition of Example 6.

[0192] The preparation method of the gel described in this comparative application example is the same as that of application example 1.

[0193] Comparative Application Example 2

[0194] This comparative application example provides an eye cream, which differs from Application Example 2 only in that the anti-wrinkle composition of Example 6 is replaced by water, that is, this comparative application example does not contain the anti-wrinkle composition of Example 6.

[0195] The preparation method of the eye cream described in this comparative application example is the same as that of application example 2.

[0196] Comparative Application Example 3

[0197] This comparative application example provides a facial cream, which differs from Application Example 3 only in that the anti-wrinkle composition of Example 6 is replaced by water, that is, this comparative application example does not contain the anti-wrinkle composition of Example 6.

[0198] The preparation method of the facial cream described in this comparative application example is the same as that of application example 3.

[0199] Test Example 4

[0200] This effect example tests the moisturizing effect of the gel obtained in Application Example 1 and Comparative Application Example 1.

[0201] Test method: According to QB / T 4256-2011 "Guidelines for Evaluation of Moisturizing Efficacy of Cosmetics", 5 volunteers aged 18-65 were included in the test, and all volunteers signed informed consent. The smear area and blank control area were marked on the inner forearms of the subjects (size: 3cm×3cm). No substance was smeared in the blank control area. The test sample was measured at (2.0±0.1)mg / cm 2 The amount of the sample was applied once, and applied to the application area respectively; the transepidermal water loss of the skin before and after use of the sample was measured by a transepidermal water loss measuring instrument VapoMeter, and the transepidermal water loss change rate (%) = (data after use - data before use) / data before use × 100%; the test results are shown in Table 8. It can be seen from the experimental data in Table 8 that the anti-wrinkle composition disclosed in the present invention has a moisturizing effect.

[0202] Table 8 Transepidermal water loss change rate

[0203] time Blank control group Comparative application example 1 Application Example 1 1h -12.50％ -16.02％ -6.09％

[0204] Test Example 5

[0205] This effect example tests the anti-wrinkle effect of the eye cream obtained in Application Example 2 and Comparative Application Example 2.

[0206] Test method: 9 volunteers aged 35-45 were included in the test, and all volunteers signed informed consent. The volunteers were arranged to use the eye cream obtained in Application Example 2 and Comparative Application Example 2 on half of their face once a day in the morning and evening for a period of 14 days. They were revisited on the first day (initial value), 7 days and 14 days, and the skin analysis system CANFIELD VISIA 7 was used to detect the proportion of wrinkle area to regional area in the selected area. The skin detector Miravex ANTERA 3D was used to detect the maximum depth of wrinkles in the selected area (maximum depression depth), the regional volume of depressions formed by wrinkles in the selected area (depression volume), and the overall evaluation of the degree of wrinkle depression in the selected area (depression index / depression score). All tests were conducted after cleaning the face with a unified cleanser and sitting for 30 minutes in an experimental environment (temperature 20±1℃, humidity 50±10%). This test example used IPP software and ANTERA CS software to analyze the data. The test results are shown in Table 9 and Figure 6-7 shown.

[0207] Table 9

[0208]

[0209] From Table 9 and Figure 6-7 It can be seen that the anti-wrinkle composition can effectively improve the depth, depression index and depression volume of wrinkles in the eye skin, and has a slight improvement on the area of ​​the eye skin.

[0210] Test Example 6

[0211] This effect example tests the moisturizing and anti-wrinkle effects of the facial cream obtained in Application Example 3 and Comparative Application Example 3.

[0212] Test method: 32 volunteers aged 35-55 were included in the test, and all volunteers signed the informed consent form. The volunteers were arranged to use the cream obtained in Application Example 3 and Comparative Application Example 3 on half of their face once a day in the morning and evening for 28 days, and were revisited on the 1st day (initial value), 14th day and 28th day. The skin moisture content was tested by the skin moisture measurement probe Corneometer@CM 825 (Courage & Khazaka, Germany), the TEWL value of the skin was tested by the transepidermal water loss probe Vapometer (Delfin, Finland), and the skin elasticity probe was used to measure the skin moisture content. Dual MPA 580 (Courage + Khazaka) detects skin elasticity, and the skin color probe Colorimeter CL400 detects skin color content. -CR (Canfield) and PRIMOS were used to detect the improvement of fine lines and crow's feet under the eyes, and the level of crow's feet and eye wrinkles was evaluated through clinical evaluation according to the "Skin Aging Atlas Volume 2 Asian Edition"; all tests were conducted after cleaning the face with a unified cleanser and sitting for 30 minutes in an experimental environment (temperature 20±1°C, humidity 50±10%). The improvement rate was used to express the efficacy of the essence, and the change rate (%) = (data after use-data before use) / data before use × 100%; this effect example used Office-EXCEL software to process and plot the data, and SPSS25.0 software was used for one-way analysis of variance (P<0.005), and all experiments were repeated 3 times. The test results are shown in Table 10 and Figure 8-11 shown.

[0213] Table 10

[0214]

[0215]

[0216] Figure 8 and Fig. 9 These are the changes in crow's feet of one of the volunteers before and after using the cream in Application Example 3; Fig.10 and Fig.11 The following are the changes in the wrinkles under the eyes of one of the volunteers before and after using the cream of Application Example 3. Figure 8-11It can be seen from the records that after using the facial cream containing the anti-wrinkle composition of the present invention, the skin water content, the skin TEWL value and the skin elasticity can be improved, while the crow's feet and wrinkles under the eyes of the skin can be reduced, indicating that the anti-wrinkle composition of the present invention has excellent moisturizing, hydrating, improving skin elasticity and anti-wrinkle effects.

[0217] Finally, it should be noted that the above embodiments are used to illustrate the technical solution of the present disclosure rather than to limit the protection scope of the present disclosure. Although the present disclosure has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present disclosure can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present disclosure.

Claims

1. An anti-wrinkle composition, characterized in that: The invention comprises the following components in parts by weight: 0.1-1.5 parts of suizenji laver polysaccharide, 0.1-70 parts of suizenji laver extract, 10-230 parts of butylene glycol, and 50-600 parts of water; The mass of the Shuiqiansi Porphyra polysaccharide is 0.05-0.5% of the mass of the anti-wrinkle composition; Taking the weight percentage of the anti-wrinkle composition as 100%, the weight percentage of the butanediol is less than 77%.

2. The anti-wrinkle composition according to claim 1, characterized in that The mass ratio of the Mizuqiansi laver polysaccharide to the Haworthia lappa extract is 1:(1-9).

3. The anti-wrinkle composition according to claim 1, characterized in that The weight average molecular weight of the Shuiqiansi laver polysaccharide is (1.2-2.0)×10 7 g / mol; and / or, the weight average molecular weight of the seaweed extract is (4.5-5.5)×10 4 g / mol.

4. The anti-wrinkle composition according to claim 1, characterized in that The total sugar content of the anti-wrinkle composition is 0.5-0.7%.

5. Use of the anti-wrinkle composition according to any one of claims 1 to 4 in the preparation of cosmetics.

6. A cosmetic, characterized in that: The cosmetic comprises the anti-wrinkle composition according to any one of claims 1 to 4.

7. The cosmetic according to claim 6, characterized in that The weight percentage of the anti-wrinkle composition in the cosmetic is 0.5-10%.

8. The cosmetic according to claim 6, characterized in that The cosmetic further comprises at least one auxiliary agent acceptable in the cosmetic field.

Images