Composition containing osmanthus extract with whitening and soothing effects, facial mask and preparation method

Abstract

The present invention relates to the technical field of daily chemical products, and specifically provides a composition containing osmanthus extract with whitening and soothing effects, a facial mask, and a preparation method. The composition of the present invention comprises, by mass, 10 to 30 parts of osmanthus extract, 10 to 30 parts of white tea polysaccharides, and 10 to 30 parts of ceramide; the solid content of the osmanthus extract is 3 to 5 mg / mL. Compared with the prior art, the present invention creatively combines osmanthus extract, white tea polysaccharides and ceramide, and achieves better antioxidant and anti-inflammatory effects through the synergistic effect of each component, solving skin problems caused by ultraviolet rays and blue light.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemical products, and more specifically to a composition containing osmanthus extract and having whitening and soothing effects, a facial mask and a preparation method thereof. Background Art

[0002] The skin is the outermost defense organ of the human body, with functions such as moisturizing and defense. However, the skin is constantly attacked by ultraviolet rays and blue light. If it is not protected, serious changes in the skin appearance may occur, such as dull skin color, pigmentation, rough skin, sagging, wrinkles, etc.

[0003] Ultraviolet rays are an important factor that causes photoaging of the skin. Ultraviolet rays can be divided into three types: UVA, UVB and UVC. Among them, UVA and UVB have the most significant effects on the skin. UVB mainly affects the surface of the skin, causing sunburn, redness, swelling, peeling, etc.; while UVA has stronger penetrating power and can penetrate deep into the skin, destroying collagen and elastic fibers, which in turn causes skin aging. UVA also stimulates melanocytes to produce more melanin, causing spots and pigmentation to worsen. In addition, exposure to UVA and UVB will cause the skin to produce a large number of free radicals, which may damage cell membranes and proteins, accelerating the process of skin aging.

[0004] Blue light is a high-energy visible light with a wavelength of 400-500nm. In addition to being present in sunlight, it is also widely present in the light emitted by computer screens, e-books, tablets, smart phones, fluorescent lamps, incandescent lamps, LEDs, etc. in daily life. Related studies have shown that when human skin is exposed to a blue light environment, the production of reactive oxygen species (ROS) will increase. Excessive reactive oxygen species will promote the production of melanin, leading to skin sagging, wrinkles, excessive pigmentation and other problems. In addition, excessive exposure to blue light can also induce skin inflammation, causing discomfort symptoms such as redness of the skin.

[0005] Therefore, in order to deal with the photoaging problem caused by ultraviolet rays and blue light, there is an urgent need for a cosmetic that can effectively repair photoaged skin. Summary of the invention

[0006] In view of this, the purpose of the present invention is to provide a composition and mask containing osmanthus extract with whitening and soothing effects, and a preparation method. The present invention creatively combines osmanthus extract, white tea polysaccharides and ceramide, and through the synergistic effect of each component, achieves better antioxidant and anti-inflammatory effects, and solves skin problems caused by ultraviolet rays and blue light.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] A composition containing osmanthus extract with whitening and soothing effects comprises, by weight: 10 to 30 parts of osmanthus extract, 10 to 30 parts of white tea polysaccharides and 10 to 30 parts of ceramide; the solid content of the osmanthus extract is 3-5 mg / mL.

[0009] Osmanthus fragrans (OSMANTHUS FRAGRANS FLOWER) is a common flower plant, also known as osmanthus fragrans and rock osmanthus. The flowers of osmanthus are small and exquisite, the petals are golden yellow, the fragrance is rich, and it has a unique aroma. In addition to its beautiful flowers, osmanthus also has certain medicinal value. Osmanthus has the effects of promoting blood circulation and removing blood stasis, harmonizing qi and blood, soothing the liver and relieving depression, and antibacterial. In traditional Chinese medicine, it is often used to regulate qi and blood, soothe emotions, and improve appetite. Osmanthus contains rich antioxidants, which can protect the skin from free radical damage and delay the aging process of the skin; osmanthus extract can be used in cosmetics as a moisturizing ingredient to help maintain the water and oil balance of the skin and keep the skin soft and moisturized.

[0010] Tea polysaccharide is a natural polysaccharide in white tea. As a natural active substance with great development potential, the functional research of white tea polysaccharide has always been the focus of researchers. A large number of functional research experiments have confirmed that white tea polysaccharide has multiple biological activities such as anti-oxidation, anti-thrombosis, hypoglycemic, hypolipidemic, immune regulation, anticoagulation, etc. In addition, white tea polysaccharide can also slow down the heart rate, increase coronary flow and resist hypoxia. Due to its various benefits, white tea polysaccharide is often used as an ingredient in health care products or an important component of traditional Chinese medicine formulas.

[0011] Ceramide is an important lipid molecule existing in mammalian cells, mainly composed of long-chain groups and fatty acids. Ceramide helps to repair and maintain the skin's natural barrier, reduce water loss, and protect the skin from external irritation and pollution; ceramide can also help the skin lock in moisture, improve the skin's moisturizing ability, and keep the skin soft and moisturized; by enhancing the skin's barrier and moisturizing function, ceramide can help slow down the skin's aging process and reduce the formation of fine lines and wrinkles; for sensitive or damaged skin, ceramide helps reduce redness and irritation and promotes skin repair. Therefore, ceramide plays an indispensable role in the skin's barrier function, helping the skin maintain moisture and protect the skin from the external environment. It is especially suitable for dry, sensitive or aging skin.

[0012] Preferably, the composition containing osmanthus extract with whitening and soothing effects comprises, by weight: 10 to 12 parts of osmanthus extract, 10 to 15 parts of white tea polysaccharides and 10 to 19 parts of ceramide.

[0013] Preferably, the CAS number of the ceramide is 100403-19-8.

[0014] Preferably, the osmanthus is golden osmanthus (Laurus nobilis L.).

[0015] Preferably, the osmanthus fragrans is flowers and / or leaves of osmanthus fragrans.

[0016] Preferably, the preparation method of the osmanthus extract comprises:

[0017] The osmanthus flowers are crushed, mixed with water, and distilled under reduced pressure to obtain a distillate, and the distillate is concentrated to a solid content of 3 to 5 mg / mL to obtain an osmanthus flower extract;

[0018] The material-liquid ratio of the osmanthus to water is 1:(10-20) g / mL; the temperature of the reduced pressure distillation is 80-90° C., the time is 2-3 hours, and the pressure is -0.09--0.1 MPa.

[0019] In one embodiment of the present invention, the preparation method of the osmanthus extract comprises:

[0020] (1) Take fresh osmanthus flowers, clean the dust on the surface of the petals, crush them, place them in a steam generator, and heat them under 90° C. steam for 10 min.

[0021] (2) placing the osmanthus powder obtained in step (1) in a distillation flask of a rotary evaporator, adding deionized water to the distillation flask, the solid-liquid ratio of osmanthus powder to deionized water being 1:10 g / mL, and then performing reduced pressure distillation at 80° C. and −0.09 MPa for 3 h, controlling the speed of the rotary evaporator to be 70 r / min; repeating the above operation, performing secondary distillation, mixing the distillates of the two distillations to obtain a mixed solution, sterilizing, concentrating, and controlling the solid content of the mixed solution to be 5 mg / mL, to obtain an osmanthus extract.

[0022] The osmanthus extract of the present invention can be used as an odor inhibitor and added into subsequent cosmetics.

[0023] Preferably, the preparation method of white tea polysaccharide comprises:

[0024] The osmanthus is crushed, mixed with water, and extracted to obtain an extract solution, wherein the solid-liquid ratio of osmanthus to water is 1: (10-15) g / mL; the extract solution is subjected to alcohol precipitation treatment and dried to obtain crude white tea polysaccharide; the crude white tea polysaccharide is mixed with water, deproteinized, and dried to obtain white tea polysaccharide.

[0025] In one embodiment of the present invention, the preparation method of white tea polysaccharide comprises: crushing white tea, adding pure water, and the solid-liquid ratio of powder to pure water is 1:10 g / mL; then, extracting at 50°C for 30 minutes; repeating the extraction 3 times to obtain an extract, adding anhydrous ethanol 4 times the volume of the extract, precipitating at 4°C for 24 hours, collecting the precipitate by centrifugation, drying to obtain crude white tea polysaccharide, redissolving in water, adding Sevage solvent 3 times the volume of the polysaccharide aqueous solution, mixing evenly, standing to separate layers, collecting the supernatant, concentrating by a rotary evaporator, and drying to obtain white tea polysaccharide.

[0026] Preferably, the whitening and soothing composition containing osmanthus extract comprises, by weight: 10 parts of osmanthus extract, 15 parts of white tea polysaccharides and 10 parts of ceramide;

[0027] Alternatively, the composition containing osmanthus extract with whitening and soothing effects comprises, by weight: 10 parts of osmanthus extract, 10 parts of white tea polysaccharide and 19 parts of ceramide;

[0028] Alternatively, the composition containing osmanthus extract with whitening and soothing effects comprises, by weight: 12 parts of osmanthus extract, 10 parts of white tea polysaccharides and 10 parts of ceramide.

[0029] The present invention also provides a method for preparing the composition containing osmanthus extract having whitening and soothing effects, comprising:

[0030] By weight, 10 to 30 parts of osmanthus extract, 10 to 30 parts of white tea polysaccharide and 10 to 30 parts of ceramide are mixed to obtain a composition containing osmanthus extract with whitening and soothing effects.

[0031] The present invention also provides the use of the composition containing osmanthus extract with whitening and soothing effects in anti-oxidation and / or melanin inhibition.

[0032] Preferably, the anti-oxidation comprises scavenging reactive oxygen species (ROS).

[0033] The present invention also provides application of the composition containing osmanthus extract and having whitening and soothing effects in inhibiting inflammatory factors.

[0034] Preferably, the inflammatory factor includes IL-18.

[0035] Preferably, the present invention also provides the use of the composition containing osmanthus extract with whitening and soothing effects in inhibiting the release of inflammatory factors by cells; the cells include RAW264.7 cells.

[0036] The present invention also provides application of the composition containing osmanthus extract and having whitening and soothing effects in improving the activity of keratinocytes.

[0037] Preferably, the concentration of the composition containing osmanthus extract having whitening and soothing effects is 0.6-2.0 mg / ml, preferably 1.0 mg / ml.

[0038] The present invention also provides a facial mask, comprising a mask cloth and the composition containing osmanthus extract having whitening and soothing effects, as well as acceptable auxiliary materials in the facial mask.

[0039] Preferably, the membrane cloth is a non-woven membrane cloth; the facial mask is composed of the membrane cloth and an essence comprising a composition containing osmanthus extract having whitening and soothing effects; the content of the composition containing osmanthus extract having whitening and soothing effects in the essence is 3-5wt%, preferably 3wt%; the auxiliary materials of the essence include one or more of a solvent, a moisturizer, and a skin conditioner.

[0040] Preferably, the solvent includes water and PEG-40 hydrogenated castor oil; the moisturizer includes one or more of betaine, acetyl glucosamine, and sodium hyaluronate.

[0041] Preferably, the skin conditioning agent includes one or more of ethyl lauroyl arginine hydrochloride (ETHYL LAUROYLARGINATE HCL), ethylhexylglycerin (ETHYLHEXYLGLYCERIN), 1,2-hexanediol (1,2-HEXANEDIOL), butylene glycol (BUTYLENE GLYCOL), p-hydroxyacetophenone (HYDROXYACETOPHENONE), allantoin (ALLANTOIN), hydroxyethyl cellulose (HYDROXYETHYLCELLULOSE), and dipotassium glycyrrhizate (DIPOTASSIUM GLYCYRRHIZATE).

[0042] Compared with the prior art, the present invention creatively combines osmanthus extract, white tea polysaccharides and ceramide to achieve better antioxidant and anti-inflammatory effects, solve chronic skin problems caused by ultraviolet rays and blue light, prevent pigmentation and photoaging, and bring multi-dimensional improvements to skin color and skin quality; moreover, the whitening and soothing composition containing osmanthus extract of the present invention has lower irritation and better soothing effect, will not cause skin allergies, and can reduce consumers' adverse reactions during use.

[0043] It has been verified by the embodiments and test examples of the present invention that the composition provided by the present invention can significantly remove active oxygen, with a removal rate of up to 70.8% to 71.9%; the composition of the present invention can also inhibit inflammatory factors, reduce the secretion of IL-18 from 2.1 ng / mL to 0.62 to 0.69 ng / mL, and significantly alleviate the problem of accelerated aging of the skin after external stimulation; the composition of the present invention can also increase the activity of keratinocytes, with a relative cell activity of up to 96.7% to 115.2%; the effect of the composition provided by the present invention when any component is missing is significantly lower than the effect when the four components are used in combination, which shows that the three components of osmanthus extract, white tea polysaccharide and ceramide are indispensable. DETAILED DESCRIPTION

[0044] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] In order to further illustrate the present invention, the following examples are used to explain it in detail. The raw materials used in the following examples and comparative examples of the present invention are all commercially available products.

[0046] The preparation method of the osmanthus extract used in the examples and comparative examples of the present invention comprises the following steps:

[0047] (1) Take fresh osmanthus fragrans (Laurus nobilis L.) flowers, clean the dust on the surface of the petals, crush them, place them in a steam generator, and heat them under 90°C steam for 10 minutes.

[0048] (2) placing the osmanthus powder obtained in step (1) in a distillation flask of a rotary evaporator, adding deionized water to the distillation flask, the solid-liquid ratio of osmanthus powder to deionized water being 1:10 g / mL, and then performing reduced pressure distillation at 80° C. and −0.09 MPa for 3 h, controlling the speed of the rotary evaporator to be 70 r / min; repeating the above operation, performing secondary distillation, mixing the distillates of the two distillations to obtain a mixed solution, sterilizing, concentrating, and controlling the solid content of the mixed solution to be 5 mg / mL, to obtain an osmanthus extract.

[0049] The preparation method of the white tea polysaccharide used in the examples and comparative examples of the present invention comprises the following steps:

[0050] The white tea was crushed and added into pure water with a solid-liquid ratio of powder to pure water of 1:10 g / mL; then, the mixture was extracted at 50°C for 30 min; the extraction was repeated 3 times to obtain an extract, anhydrous ethanol 4 times the volume of the extract was added, and the mixture was precipitated at 4°C for 24 h. The precipitate was collected by centrifugation and dried to obtain crude white tea polysaccharide, which was redissolved in water, and Sevage solvent (chloroform-water) 3 times the volume of the polysaccharide aqueous solution was added, mixed evenly, allowed to stand for stratification, the supernatant was collected, concentrated by a rotary evaporator, and dried to obtain white tea polysaccharide.

[0051] The preparation method of the white tea extract used in the comparative example of the present invention comprises the following steps:

[0052] The white tea was crushed, immersed in pure water, and refluxed for extraction, with the solid-liquid ratio of white tea powder to pure water being 1:10 g / mL; reflux extraction was performed 3 times, with each extraction time being 30 min and the temperature being 50°C to obtain an extract, which was concentrated under reduced pressure and dried to obtain a white tea extract.

[0053] The yellow tea polysaccharide used in the comparative example of the present invention is prepared by the following steps:

[0054] The yellow tea was crushed and added into pure water with a solid-liquid ratio of powder to pure water of 1:10 g / mL; then, the mixture was extracted at 50°C for 30 min; the extraction was repeated 3 times to obtain an extract, 4 times the volume of anhydrous ethanol was added into the extract, and the mixture was precipitated at 4°C for 24 h. The precipitate was collected by centrifugation and dried to obtain crude yellow tea polysaccharide, which was redissolved in water, and 3 times the volume of Sevage solvent (chloroform-water) of the polysaccharide aqueous solution was added, the mixture was mixed evenly, and the mixture was allowed to stand for stratification. The supernatant was collected and concentrated by a rotary evaporator and dried to obtain yellow tea polysaccharide.

[0055] Example 1

[0056] The composition containing osmanthus extract with whitening and soothing effects of this embodiment is composed of the following components: 10 g of osmanthus extract, 15 g of white tea polysaccharide, and 10 g of ceramide (CAS No. 100403-19-8).

[0057] The preparation method of the above-mentioned composition containing osmanthus extract with whitening and soothing effects comprises: mixing 10g of osmanthus extract, 15g of white tea polysaccharide and 10g of ceramide, and stirring evenly to obtain the composition containing osmanthus extract with whitening and soothing effects.

[0058] Example 2

[0059] The composition containing osmanthus extract with whitening and soothing effects of this embodiment is composed of the following components: 10 g of osmanthus extract, 10 g of white tea polysaccharide, and 19 g of ceramide.

[0060] Example 3

[0061] The composition containing osmanthus extract with whitening and soothing effects of this embodiment is composed of the following components: 12g of osmanthus extract, 10g of white tea polysaccharide, and 10g of ceramide.

[0062] Comparative Example 1

[0063] The composition of this comparative example is different from that of Example 1 in that this comparative example only includes osmanthus extract and white tea polysaccharide, and the missing amount of ceramide is distributed to the corresponding components according to the ratio of the other two substances.

[0064] Comparative Example 2

[0065] The composition of this comparative example is different from that of Example 1 in that this comparative example only includes white tea polysaccharides and ceramide, and the missing amount of osmanthus extract is distributed to the corresponding components according to the ratio of the other two substances.

[0066] Comparative Example 3

[0067] The composition of this comparative example is different from that of Example 1 in that this comparative example only includes osmanthus extract and ceramide, and the missing amount of white tea polysaccharide is distributed to the corresponding components according to the ratio of the other two substances.

[0068] Comparative Example 4

[0069] The composition of this comparative example is different from that of Example 1 in that 15 g of white tea polysaccharide is replaced by 15 g of white tea extract.

[0070] The other components and contents remained unchanged.

[0071] Comparative Example 5

[0072] The composition of this comparative example is different from that of Example 1 in that 15 g of white tea polysaccharide is replaced by 15 g of yellow tea polysaccharide.

[0073] The other components and contents remained unchanged.

[0074] Application Examples 1-3

[0075] The composition prepared in Examples 1-3 was made into a facial mask, which consisted of a non-woven membrane cloth and an essence. The raw material formula of the essence was as shown in Table 1.

[0076] Table 1

[0077]

[0078]

[0079] Test Example 1

[0080] Reactive oxygen species (ROS) refers to a general term for oxygen-containing free radicals and peroxides that are easily formed into free radicals and are related to oxygen metabolism in the body. This test example explores the inhibitory effect of the composition obtained in the embodiment and the comparative example on ROS, specifically comprising the following steps:

[0081] (1) Cell seeding: Cells were seeded into 6-well plates at a density of 1.8E5 / well and incubated in an incubator (5% CO 2 , 37°C) for 24 h.

[0082] (2) Experimental grouping: The experiment set up a blank control group (BC), a negative control group (NC) and a sample group, and each group was set up with 3 replicate wells.

[0083] (3) Liquid preparation: The compositions of the examples and comparative examples were added into the cell culture medium to prepare the sample group working solutions.

[0084] (4) Administration: Administration was performed when the cell plating rate in the 6-well plate reached 40%. 2 mL of cell culture medium was added to each well of the blank control group and the negative control group; 2 mL of culture medium containing the test substance diluted 4 times was added to each well of the sample group; after administration, the 6-well plate was cultured in an incubator.

[0085] (5) After the cells were cultured for 24 h, they were washed with PBS, 1 mL of the probe DCFH-DA was added to each well, and the cells were incubated in a cell culture incubator for 20 min. The culture medium containing DCFH-DA was discarded, and the cells were washed three times with PBS.

[0086] (6) Blue light irradiation: After washing the cells with PBS, each group received a total dose of 10 J / cm 2 At the same time, the blank control group was placed in the same environment without blue light irradiation. After the irradiation, fresh culture was replaced and the 6-well plate was incubated in a cell culture incubator for 30 minutes.

[0087] (7) Detection: After the cells have been incubated for 30 minutes, the supernatant is discarded, the cells are digested with trypsin and collected, the cells are washed once with PBS, and 500 μL of PBS is added to resuspend the cells for testing. Turn on the flow cytometer, establish a FSC / SSC dual-parameter scatter plot to select cells, and at the same time, establish a FITC single-parameter histogram; the resuspended cells of different groups of samples are tested on the machine, and the FITC channel fluorescence signal intensity (MFI) of each group of samples is recorded.

[0088] ROS clearance rate of each sample group = (MFI value of NC group - MFI value of sample group) / MFI value of NC group * 100%

[0089] The test results are shown in Table 2.

[0090] Table 2

[0091] Group ROS clearance rate (%) Example 1 70.8 Example 2 71.5 Example 3 71.9 Comparative Example 1 53.6 Comparative Example 2 58.7 Comparative Example 3 54.3 Comparative Example 4 62.1 Comparative Example 5 63.5

[0092] It can be seen from Table 2 that, compared with the comparative example, the composition of the embodiment of the present invention can significantly inhibit ROS.

[0093] Test Example 2

[0094] This test example explores the inhibitory effect of the composition obtained in the embodiment and the comparative example on melanin, and specifically includes the following steps:

[0095] (1) The compositions of the examples and comparative examples were respectively used as sample groups, and physiological saline was used as a blank group.

[0096] (2) B-16 melanoma cells were cultured with 0.1 wt% glucosamine, and theophylline was added to promote the cells to recover to the state of melanin synthesis. The composition of the embodiment and the comparative example was added to the culture medium, and the color tone of the cell particles was examined under a microscope to determine the inhibitory effect of the samples on the new melanin.

[0097] (3) The cell particles were centrifuged to release the intracellular particles, and the absorbance was measured at 420 nm. The calculation formula for the inhibition rate of melanin was as follows. The results are shown in Table 3.

[0098] Inhibition rate*100%=100%-absorbance of sample intervention / absorbance of sample without intervention*100%

[0099] Table 3

[0100]

[0101]

[0102] It can be seen from Table 3 that, compared with the comparative example, the composition of the embodiment of the present invention has a higher suppression rate on the black rate.

[0103] Test Example 3

[0104] This test example explores the inhibitory effect of the composition obtained in the embodiment and the comparative example on the inflammatory factor IL-18, and specifically includes the following steps:

[0105] (1) Cell seeding: RAW264.7 cells were seeded at 4500 cells / well in a 96-well plate and placed at 37°C and 5% CO 2 Culture in an incubator for 24 h.

[0106] (2) Experimental grouping: a blank group and a sample group were set up, and the test substances in the sample group were the compositions prepared in the examples and comparative examples.

[0107] (3) LPS-induced inflammatory response: 1 mg / mL LPS solution (solvent: PBS buffer) was diluted to 2 μg / mL, added to the well plate at 50 μL / well, and placed in an incubator for culture.

[0108] (4) Sample addition: Prepare sample working solution using complete culture medium as diluent, 100 μL / well. Add equal amount of complete culture medium to negative control group and positive control group and culture in an incubator for 24 h.

[0109] (5) Take the supernatant from the well plate and detect the IL-18 content by ELISA method. The operation steps are as follows:

[0110] A. Calculate the required enzyme label strips in advance, take out the kit and restore it to room temperature before the experiment;

[0111] B. Serial dilution of standard: dilute the standard with standard & sample diluent to 500, 250, 125, 62.5, 0 ng / mL;

[0112] C. Collect the cell culture supernatant into a sterile centrifuge tube and centrifuge to obtain the supernatant;

[0113] D. Add standard working solution and test samples to each reaction well, set up 2 replicate wells for each group, and incubate;

[0114] E. Discard the liquid, spin dry, add biotin-labeled antibody working solution to each reaction well, and incubate;

[0115] F. Discard the liquid, spin dry, add washing solution to each reaction well, soak and spin dry;

[0116] G. Add HRP-labeled streptavidin working solution to each reaction well and incubate;

[0117] H. Add washing solution to each reaction well, and spin dry the washing solution after 30 seconds.

[0118] I. Add the color developing agent to each reaction well and develop the color at 37°C in the dark for 15 minutes;

[0119] J. Add stop solution to each reaction well and measure the OD value at 450nm using a microplate reader;

[0120] K. Calculate the standard curve regression equation (R) based on the known concentration of the standard and the measured OD value. 2 >0.99), the OD value of the sample well was substituted into the concentration of the sample to be measured, and then multiplied by the dilution factor to obtain the actual IL-18 concentration of the original sample. The test results are shown in Table 4.

[0121] Table 4

[0122] Group IL-18 secretion (ng / mL) Blank Group 2.1 Example 1 0.64 Example 2 0.62 Example 3 0.69 Comparative Example 1 1.59 Comparative Example 2 1.64 Comparative Example 3 1.61 Comparative Example 4 1.32 Comparative Example 5 1.04

[0123] It can be seen from Table 4 that, compared with the comparative example, the composition provided in the example has a significant inhibitory effect on the inflammatory factor IL-18.

[0124] Test Example 4

[0125] Keratinocytes (KC) are the main functional and structural components of the epidermis, which is the outermost layer of the skin and is specifically used to defend against external factors, prevent body fluid leakage, and maintain water in cells. This test example explores the effect of the composition obtained in the examples and comparative examples on the promotion of keratinocyte activity, specifically comprising the following steps:

[0126] (1) Test method: Calcein-AM fluorescent probe was used to label living cells for in vitro keratinocyte activity detection;

[0127] (2) Test steps: After the keratinocytes were counted, they were diluted to 5×10 4 100 μL was inoculated into a 96-well cell culture plate and incubated in 5% CO 2 , incubate in a 37°C incubator in the dark for 12 hours, and the blank group does not need to add keratinocytes; after absorbing and discarding the culture medium, the experimental group adds 100 μL of the composition of the embodiment and the comparative example at different concentrations, and the blank group and the control group add 100 μL of culture medium, and incubate in a 37°C incubator in the dark for 2 hours; add 100 μL of 3×Calcein-AM solution, and incubate in the incubator in the dark for 30 minutes. After the incubation, the fluorescence is detected and the cell activity is calculated using a fluorescence microplate reader (excitation wavelength 494nm, emission wavelength 517nm), and the cell activity is proportional to the fluorescence intensity.

[0128] (3) Calculate the keratinocyte activity rate using the following formula:

[0129] Keratinocyte activity rate = [(OD 实验组 -OD 空白组 ) / (OD 对照组 -OD 空白组 )]×100%

[0130] The test results are shown in Table 5.

[0131] Table 5

[0132] Group Concentration (mg / ml) Relative cell activity (%) Example 1 0.6 96.7 Example 1 0.8 104.3 Example 1 1.0 115.2 Example 1 2.0 102.1 Comparative Example 1 1.0 82.6 Comparative Example 2 1.0 79.3 Comparative Example 3 1.0 80.4 Comparative Example 4 1.0 89.2 Comparative Example 5 1.0 86.5

[0133] It can be seen from Table 5 that, compared with the comparative example, the composition provided in Example 1 has higher activity on keratinocytes at a concentration of 0.6-2.0 mg / ml, among which the composition of Example 1 at a concentration of 1.0 mg / ml has the best effect.

[0134] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A composition containing osmanthus extract having whitening and soothing effects, characterized in that: The composition comprises, by weight: 10 to 30 parts of osmanthus extract, 10 to 30 parts of white tea polysaccharide and 10 to 30 parts of ceramide; the solid content of the osmanthus extract is 3 to 5 mg / mL; The preparation method of the osmanthus extract comprises: The osmanthus flowers are crushed, mixed with water, and distilled under reduced pressure to obtain a distillate, and the distillate is concentrated to a solid content of 3 to 5 mg / mL to obtain an osmanthus flower extract; The material-liquid ratio of the osmanthus to water is 1:(10-20) g / mL; the temperature of the reduced pressure distillation is 80-90° C., the time is 2-3 hours, and the pressure is -0.09--0.1 MPa.

2. The composition containing osmanthus fragrans extract with whitening and soothing effects according to claim 1, characterized in that: The invention comprises, by weight: 10 to 12 parts of osmanthus extract, 10 to 15 parts of white tea polysaccharide and 10 to 19 parts of ceramide.

3. The composition containing osmanthus fragrans extract with whitening and soothing effects according to claim 1, characterized in that: In parts by mass, it comprises: 10 parts of osmanthus extract, 15 parts of white tea polysaccharide and 10 parts of ceramide; Alternatively, the composition containing osmanthus extract with whitening and soothing effects comprises, by weight: 10 parts of osmanthus extract, 10 parts of white tea polysaccharide and 19 parts of ceramide; Alternatively, the composition containing osmanthus extract with whitening and soothing effects comprises, by weight: 12 parts of osmanthus extract, 10 parts of white tea polysaccharides and 10 parts of ceramide.

4. The method for preparing the composition containing osmanthus extract with whitening and soothing effects as claimed in claim 1, characterized in that: include: By weight, 10 to 30 parts of osmanthus extract, 10 to 30 parts of white tea polysaccharide and 10 to 30 parts of ceramide are mixed to obtain a composition containing osmanthus extract with whitening and soothing effects.

5. Use of the composition containing osmanthus extract with whitening and soothing effects as claimed in any one of claims 1 to 3 in the preparation of antioxidant and / or melanin inhibition products.

6. Use of the composition containing osmanthus extract with whitening and soothing effects as claimed in any one of claims 1 to 3 in the preparation of products for inhibiting inflammatory factors.

7. The use according to claim 6, characterized in that: The inflammatory factors include IL-18.

8. Use of the composition containing osmanthus extract with whitening and soothing effects as claimed in any one of claims 1 to 3 in the preparation of a product for improving the activity of keratinocytes.

9. A facial mask, characterized in that: The invention comprises the composition containing osmanthus extract and having whitening and soothing effects as described in any one of claims 1 to 3, and an auxiliary material acceptable in a facial mask.

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