An experimental apparatus for anesthetizing fruit flies

Abstract

This invention discloses an experimental apparatus for anesthetizing fruit flies, belonging to the field of instrumentation. The apparatus includes a transparent box with an internal partition dividing the interior into a purification chamber and an operating chamber. The partition has several absorption holes and a removable hole-covering plate. Embedded gloves are located on the left and right sides of the box. A removable absorption box is located at the top of the purification chamber. The operating chamber has a fly inlet tube and an anesthesia tube at the top, and a fly outlet tube at the bottom. The fly inlet tube also contains a culture bottle inlet mechanism. This experimental apparatus can create an ether environment for anesthetizing fruit flies, keeping them in a low-anesthesia state and preventing them from waking up and flying away during the fly selection / sorting process. The absorption box absorbs the ether after the experiment, preventing direct release into the atmosphere and avoiding direct inhalation of toxic gases by the experimenter, thus protecting the physical and mental health of the experimenter.

Description

Technical Field

[0001] This invention relates to the field of fruit fly experimental equipment, and more particularly to an experimental apparatus for anesthetizing fruit flies. Background Technology

[0002] In experimental teaching, fruit flies are widely used as a classic model organism in various experiments. During teaching, when selecting fruit fly parents for hybridization or observing fruit fly traits, the flies must first be anesthetized to render them inactive. Carbon dioxide and ether are the most commonly used anesthetics for fruit flies. However, the equipment for preparing carbon dioxide is expensive and not easily adopted in student experiments; therefore, ether remains the most widely used anesthetic. Ether is widely used in experiments as an anesthetic for collecting fruit flies for microscopic observation. Current methods of fruit fly anesthesia have the following problems:

[0003] 1. Ether is a toxic substance, and the teaching venue lacks effective ventilation equipment. Experimenters observe and select fruit flies while ether is continuously evaporating, which seriously affects their physical and mental health.

[0004] 2. During the teaching process, before anesthetizing fruit flies, it is necessary to transfer the fruit flies to a culture medium-free bottle. The transfer of fruit flies between different containers not only requires a high degree of alignment of the bottle mouths, but also a high degree of coordination between the operator's left and right hands. During the transfer of fruit flies, they are also prone to "escape" and fly away when "pulling the stopper, closing the bottle, and turning it over". Summary of the Invention

[0005] In view of the above-mentioned shortcomings of the prior art, the present invention provides an experimental device for anesthetizing fruit flies.

[0006] To achieve the above-mentioned objectives, the technical solution adopted by this invention is as follows:

[0007] An experimental apparatus for anesthetizing fruit flies is provided, comprising a transparent box with an internal partition dividing the box into a purification chamber and an operating chamber. The partition has several first absorption holes and a removable hole-blocking plate magnetically connected to it. Operating openings are located on the left and right sides of the box, each with an embedded glove that is sealed to the opening. A strip-shaped opening at the top of the purification chamber houses a removable absorption box. The operating chamber has an inlet tube and an anesthesia tube at its top, and an outlet tube at its bottom. The inlet and outlet tubes have the same diameter, and the inlet tube contains a culture bottle inlet mechanism.

[0008] Furthermore, the culture bottle inlet mechanism includes a lower limiting ring, an upper movable ring, a spring, a straight bracket, a support rod, and an umbrella-shaped through-and-off part; the lower limiting ring is fixedly connected to the fly inlet tube, the bottom of the spring is fixedly connected to the lower limiting ring, the top of the spring is fixedly connected to the upper movable ring, the upper movable ring slides inside the fly inlet tube, the straight bracket is fixed inside the upper movable ring, the top of the support rod is fixedly connected to the straight bracket, and the bottom of the support rod is fixedly connected to the umbrella-shaped through-and-off part; when the spring is in its normal state, the umbrella-shaped through-and-off part connects the fly inlet tube to the purification chamber.

[0009] Furthermore, the rubber material of the umbrella-shaped through-hole has a projected area that is larger than the projected area of ​​the fly inlet tube when viewed from above.

[0010] Furthermore, the shielding plate is made of iron, and a rubber layer is provided on the side of the shielding plate that is in contact with the first absorption hole. Four magnets are evenly distributed on the outside of the several first absorption holes. The magnets are embedded in the partition plate. The shielding plate is attracted to the partition plate by the magnets to block the several first absorption holes.

[0011] Furthermore, the absorption box includes an absorption box body and a cover body. The top of the absorption box body is provided with a cover opening that mates with the cover body. The cover body is inserted and fixed to the absorption box body through the cover opening. The cover body and the cover opening are sealed together. Several second absorption holes are provided on both the front and rear sides of the absorption box body.

[0012] Furthermore, a sealing rubber ring is provided between the absorption box and the strip opening, and the sealing rubber ring is sealed to both the strip opening and the absorption box.

[0013] Furthermore, the fly inlet tube is provided with a first cap, and the fly inlet tube is threadedly connected to the first cap; the anesthesia tube is provided with a second cap, and the anesthesia tube is threadedly connected to the second cap; the fly outlet tube is provided with a third cap, and the fly outlet tube is threadedly connected to the third cap; and the inner top of the first cap, the second cap, and the third cap are all provided with sealing rubber gaskets.

[0014] Furthermore, the absorption box contains an absorbent, which is activated carbon.

[0015] Furthermore, a bottom plate is provided at the bottom of the box, and a guide slope is provided on the side of the bottom plate near the fly outlet tube. The fly outlet tube is set at an angle downward, and the bottom of the guide slope is connected to the inlet side of the fly outlet tube.

[0016] Furthermore, the front of the container is provided with a circular front opening, and a front hatch is provided on the front opening. The front opening and the front hatch are sealed together. One end of the front hatch is hinged to the container. A rubber sealing ring is provided on the outside of the front hatch to engage with the front opening.

[0017] The beneficial effects of this invention are as follows:

[0018] The operating chamber of this invention is equipped with a fly inlet tube, an anesthesia tube, and a fly outlet tube. A culture bottle connection mechanism is installed inside the fly inlet tube, allowing the fly inlet tube to connect with a fruit fly culture bottle. This mechanism also allows the fly culture bottle to be used to disconnect the fly inlet tube from the operating chamber, facilitating the transfer of fruit flies from the culture bottle into the operating chamber. Using a dropper and anesthesia tube, ether can be dripped or atomized into the operating chamber to anesthetize the fruit flies inside. Once the fruit flies are anesthetized, the base plate, guide slope, and fly outlet tube facilitate the transfer of the fruit flies. Throughout the entire process of introducing, anesthetizing, and extracting fruit flies, the operating chamber has a small area of ​​contact with the outside, effectively preventing ether leakage.

[0019] The box of the present invention is provided with a partition, which divides the interior of the box into a purification chamber and an operating chamber. The partition is provided with a number of absorption holes. The absorption holes can be blocked by a hole-blocking plate. After the hole-blocking plate is removed from the absorption holes, the ether in the operating chamber can be absorbed by the absorption box in the purification chamber, thereby facilitating the treatment of the ether in the box after the experiment.

[0020] The experimental apparatus of this invention does not require operations such as "squeezing the interface and flipping the two culture bottles upside down", which saves the use of many culture bottles during the transfer process and reduces the difficulty of transfer and anesthesia operations.

[0021] The experimental apparatus of this invention can create an ether environment that anesthetizes fruit flies, keeping them in a state of low anesthesia and preventing them from waking up and flying away during the process of distinguishing / selecting fruit flies.

[0022] The absorption box of this invention can absorb ether after the experiment, avoiding direct release into the atmosphere and preventing toxic gases from being directly inhaled by the experimenters, thus protecting the physical and mental health of the experimenters.

[0023] This invention is easy to move and has good portability. It can be placed on the laboratory table when in use and can be easily stored when not in use, effectively avoiding waste of the laboratory table.

[0024] The present invention has high operational feasibility and adaptability. It can be directly installed on a common dissecting endoscope for combined use, thereby improving the utilization rate of experimental equipment and promoting the transformation of experimental equipment functions from single to multiple.

[0025] This invention is simple to operate and relatively inexpensive, and can effectively solve the problem of some teaching laboratories not having fume hoods or having a serious shortage of them. It can be widely used in undergraduate teaching to improve teaching effectiveness.

[0026] The anesthetic tube of this invention can drip anesthetic in multiple doses to ensure that the anesthetic is used appropriately and in the right amount, so as not to over-anesthetize the fruit flies and to ensure that the fruit flies do not wake up prematurely before the experiment is completed, thereby ensuring the stability of the experimental samples and the accuracy of the experimental results.

[0027] This invention features a left and a right embedded glove, both of which are embedded acid and alkali resistant gloves. When using the device, students can wear an additional layer of ordinary gloves before inserting their hands into the device for operation. The embedded gloves prevent direct contact between the skin and the anesthetic, facilitate the use of both hands in the high-permeability chamber for related experimental operations, and also prevent excessive ether gas from diffusing and evaporating into the atmosphere. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the overall structure of the present invention viewed from above;

[0029] Figure 2 This is a schematic diagram of the overall structure of the present invention viewed from below;

[0030] Figure 3 This is a front view of the present invention;

[0031] Figure 4 for Figure 3 AA section view in the middle;

[0032] Figure 5 This is a top view of the present invention;

[0033] Figure 6 for Figure 4 BB section view in the middle;

[0034] Figure 7 This is a schematic diagram of the structure of the culture bottle inlet mechanism;

[0035] Figure 8 Top view of the culture bottle inlet mechanism;

[0036] Figure 9 for Figure 8 CC section view in the middle;

[0037] Figure 10 This is a top view of the anesthesia tube;

[0038] Figure 11 for Figure 10 DD section view in the middle;

[0039] Figure 12 This is a schematic diagram of the absorption box structure;

[0040] The symbols for the main components in the diagram are explained below:

[0041] 1. Box body; 11. Partition; 111. First absorption hole; 112. Shielding plate; 12. Purification chamber; 121. Strip opening;

[0042] 13. Operating chamber; 131. Fly inlet tube; 132. Anesthesia tube; 133. Fly outlet tube; 134. First cap; 135. Second cap; 136. Third cap; 137. Conical liquid inlet section;

[0043] 14. Left embedded glove; 15. Right embedded glove; 16. Front opening; 17. Front hatch; 18. Floor plate; 19. Guide ramp;

[0044] 2. Culture bottle inlet mechanism; 21. Lower limit ring; 22. Upper movable ring; 23. Spring; 24. I-shaped bracket; 25. Support rod; 26. Umbrella-shaped through-stop section;

[0045] 3. Absorption box; 31. Absorption box body; 32. Cover; 33. Second absorption hole; 34. Sealing rubber ring. Detailed Implementation

[0046] The specific embodiments of the present invention are described below to enable those skilled in the art to understand the present invention. However, it should be understood that the present invention is not limited to the scope of the specific embodiments. For those skilled in the art, various changes are obvious as long as they are within the spirit and scope of the present invention as defined and determined by the appended claims. All inventions utilizing the concept of the present invention are protected.

[0047] like Figure 1 , 2As shown in Figures 3, 4, 5, and 6, the experimental apparatus for anesthetizing fruit flies includes a transparent box 1. Inside the box 1, a partition 11 divides the interior into a purification chamber 12 and an operating chamber 13. A strip-shaped opening 121 is located at the top of the purification chamber 12, and a removable absorption box 3 is housed within this opening. The purification chamber 12 accommodates the absorption box 3, which contains an absorbent, preferably sand, inert fiber material, or activated carbon. After the fruit flies are anesthetized, the absorption box 3, in conjunction with the absorbent, adsorbs the ether within the box 1, thus achieving the adsorption treatment of the ether in the box 1 after the experiment. The partition 11 is provided with a plurality of first absorption holes 111, and a detachable hole-blocking plate 112 is provided on the partition 11. The hole-blocking plate 112 is magnetically connected to the partition 11. Through the detachable hole-blocking plate 112 and the partition 11, the purification chamber 12 and the operating chamber 13 can be connected or disconnected, which facilitates the adsorption of ether by the absorbent in the purification chamber 12 after the fruit fly anesthesia operation. The left and right sides of the box body 1 are provided with operating openings, and both operating openings are provided with embedded gloves. The operating opening on the left side is provided with a left embedded glove 14, and the operating opening on the right side is provided with a right embedded glove 15. Both the left embedded glove 14 and the right embedded glove 15 are sealed to the operating openings. The embedded gloves facilitate the operation of the experimenter inside the box body 1 and facilitate the transfer of the anesthetized fruit flies. The operating chamber 13 has a fly inlet tube 131 and an anesthesia tube 132 at its top and a fly outlet tube 133 at its bottom. The fly inlet tube 131 and the fly outlet tube 133 have the same diameter. A culture bottle connection mechanism 2 is also provided inside the fly inlet tube 131. The culture bottle connection mechanism 2 connects the fly inlet tube 131 to a fruit fly culture bottle. Simultaneously, the fruit fly culture bottle, in conjunction with the culture bottle connection mechanism 2, allows for the connection and disconnection between the fly inlet tube 131 and the operating chamber 13, thereby transferring fruit flies from the fruit fly culture bottle to the operating chamber 13 for anesthesia. A limiting ring is provided on the inner wall of the outlet side of the fly outlet tube 133. The limiting ring restricts the insertion depth of the fruit fly culture bottle, thus allowing the anesthetized fruit flies to be transferred from the operating chamber 13 via the fruit fly culture bottle connected to the fly outlet tube 133.

[0048] like Figure 7 , 8As shown in Figure 9, the culture bottle inlet mechanism 2 includes a lower limiting ring 21, an upper movable ring 22, a spring 23, a straight bracket 24, a support rod 25, and an umbrella-shaped switching part 26. The lower limiting ring 21 is fixedly connected to the fly inlet tube 131. The bottom of the spring 23 is fixedly connected to the lower limiting ring 21, and the top of the spring 23 is fixedly connected to the upper movable ring 22. The upper movable ring 22 can slide within the fly inlet tube 131. The straight bracket 24 is fixed within the upper movable ring 22. The top of the support rod 25 is fixedly connected to the straight bracket 24, and the bottom of the support rod 25 is fixedly connected to the umbrella-shaped switching part 26. When the spring 23 is in its normal state, the umbrella-shaped switching part 26 connects the fly inlet tube 131 to the purification chamber 12. When the mouth of the fruit fly culture bottle is inserted into the fly inlet tube 131, the mouth of the fruit fly culture bottle slides downwards within the fly inlet tube 131. The mouth of the fruit fly culture bottle drives the upper movable ring 22 to move downwards. The upper movable ring 22 drives the straight bracket 24, the support rod 25, and the umbrella-shaped cut-off part 26 to move downwards together. During the downward movement of the upper movable ring 22, the gap between the umbrella-shaped cut-off part 26 and the fly inlet tube 131 gradually increases. When the upper movable ring 22 is pressed to the bottom, the gap between the umbrella-shaped cut-off part 26 and the fly inlet tube 131 reaches its maximum. At this gap, it is easier for the fruit flies to move into the operating chamber 13. After all the fruit flies in the fruit fly culture bottle have been transferred into the operating chamber 13, the fruit fly culture bottle is removed from the fly inlet tube 131. The upper movable ring 22 returns to its highest position under the action of the spring 23. The upper movable ring 22 drives the straight bracket 24, the support rod 25, and the umbrella-shaped switch 26 to move together to the highest position. Thus, the reset action of the spring 23 causes the umbrella-shaped switch 26 to cover the bottom of the fly inlet tube 131, preventing the fruit flies in the operating chamber 13 from flying out of the fly inlet tube 131. The culture bottle connection mechanism 2 can both connect the fruit fly culture bottle to the fly inlet tube 131 and use the movement of the fruit fly culture bottle to open and close the connection between the fly inlet tube 131 and the operating chamber 13, thus facilitating the transfer of fruit flies from the fruit fly culture bottle into the operating chamber 13.

[0049] The umbrella-shaped through-hole 26 is preferably made of rubber, and its projected area in the top view is larger than that of the fly inlet tube 131. The rubber material allows the umbrella-shaped through-hole 26 to better fit the bottom of the fly inlet tube 131. The hole-blocking plate 112 is an iron plate, with a rubber layer on the side that contacts the first absorption holes 111. Four magnets are evenly distributed on the outside of the first absorption holes 111, and the magnets are embedded in the partition plate 11. The hole-blocking plate 112 is attached to the partition plate 11 by the magnets, thus blocking the first absorption holes 111.

[0050] like Figure 10 and 11As shown, a conical liquid inlet 137 can also be provided at the bottom of the anesthesia tube 132. The anesthesia tube 132 and the conical liquid inlet 137 are designed as a single unit. A liquid inlet hole is provided at the bottom of the conical liquid inlet 137, and an ultrasonic atomizing plate can also be provided at the liquid inlet hole. The ultrasonic atomizing plate is used to rapidly atomize the ether into the chamber 1, thereby evenly dispersing the ether throughout the chamber 1, which facilitates the anesthesia of fruit flies. The conical liquid inlet 137 prevents the evaporation and overflow of ether, thus providing a better ether environment within the operating chamber 13.

[0051] like Figure 12 As shown, the absorption box 3 includes an absorption box body 31 and a cover body 32. The top of the absorption box body 31 is provided with a cover opening that cooperates with the cover body 32. The cover body 32 is inserted and fixed to the absorption box body 31 through the cover opening. The cover body 32 and the cover opening are sealed together. Several second absorption holes 33 are provided on the front and rear sides of the absorption box body 31.

[0052] A sealing rubber ring 34 is provided between the absorption box 3 and the strip opening 121, and the sealing rubber ring 34 is sealed to both the strip opening 121 and the absorption box 3. The sealing rubber ring 34 is U-shaped, and both the inner and outer rings of the sealing rubber ring 34 are provided with upper and lower opening edges. The upper and lower opening edges further improve the sealing performance between the sealing rubber ring 34, the strip opening 121, and the absorption box 3, thereby ensuring the airtightness of the purification chamber 12.

[0053] A first cap 134 is provided on the fly inlet tube 131, and the fly inlet tube 131 is threadedly connected to the first cap 134; a second cap 135 is provided on the anesthesia tube 132, and the anesthesia tube 132 is threadedly connected to the second cap 135; a third cap 136 is provided on the fly outlet tube 133, and the fly outlet tube 133 is threadedly connected to the third cap 136. Sealing rubber gaskets are provided on the inner top of the first cap 134, the second cap 135, and the third cap 136. Through the cooperation of the fly inlet tube 131, the anesthesia tube 132, the fly outlet tube 133, and the first cap 134, the second cap 135, and the third cap 136, a sealed connection is achieved within the operating chamber 13, simultaneously allowing the insertion of fruit flies and the introduction of ether, ensuring a sealed anesthesia space within the operating chamber 13.

[0054] The bottom of the box 1 is provided with a base plate 18, and a guide slope 19 is provided on the side of the base plate 18 near the fly outlet tube 133. The fly outlet tube 133 is set at an angle downwards, and the bottom of the guide slope 19 is connected to the inlet side of the fly outlet tube 133. After the fruit fly is anesthetized in the operating chamber 13, a silicone sheet or a brush is used to push the fruit fly to the guide slope 19 of the base plate 18, so as to facilitate the transfer of the anesthetized fruit fly by using the guide slope 19 in conjunction with the fly outlet tube 133.

[0055] The front of the housing 1 has a circular front opening 16, and a front cover 17 is mounted on the front opening 16. The front opening 16 and the front cover 17 are sealed together. One end of the front cover 17 is hinged to the housing 1, and a rubber sealing ring is provided on the outside of the front cover 17 to engage with the front opening 16. Opening the front cover 17 allows for cleaning and disinfection of the interior of the housing 1, enabling reuse. The front cover 17 and the front opening 16 provide a good seal, ensuring a sealed anesthesia environment within the operating chamber 13. Opening the front cover 17 also facilitates the pre-insertion of operating tools (such as fruit fly tweezers, paintbrushes, etc.), and the tools can be easily removed after use.

[0056] After use, the fly outlet tube 133 can be connected to a filter device to prevent the volatilization of ether gas from causing air pollution and to protect the health of the operators.

[0057] Working process and principle: Before performing anesthesia using the experimental device for anesthetizing fruit flies, the second cap 135 and the third cap 136 are first fixed to the anesthesia tube 132 and the fly exit tube 133, respectively; then the occluder plate 112 is attached to the partition plate 11 to cover several first absorbers 111. When performing anesthesia using the experimental apparatus for anesthetizing fruit flies, remove the first cap 134 from the fly inlet tube 131, invert the mouth of the fruit fly culture bottle and insert it into the top of the fly inlet tube 131, then move the fruit fly culture bottle downwards. Use the mouth of the fruit fly culture bottle to move the upper movable ring 22 until the upper movable ring 22 moves to the bottom position. At this time, the upper movable ring 22 moves the straight bracket 24, support rod 25, and umbrella-shaped cut-off part 26 to the bottom. The bottom of the fly inlet tube 131 and the umbrella-shaped cut-off part 26 leave the largest gap. After the fruit flies in the fruit fly culture bottle enter the fly inlet tube 131, they can continue to enter the operating chamber 13. When the fruit fly culture bottle can no longer move down, it can be driven into the operating chamber 13 by patting or applying a hot towel to the outside of the fruit fly culture bottle, thereby improving the efficiency of driving the fruit flies in the fruit fly culture bottle. Once there are no more fruit flies in the fruit fly culture bottle, the bottle can be moved upwards to remove the previous section. After the upper movable ring 22 removes the squeezing force from the fruit fly culture bottle, the spring 23 resets the upper movable ring 22, causing the straight bracket 24, support rod 25, and umbrella-shaped cut-off part 26 to move to the highest point. At this time, the umbrella-shaped cut-off part 26 covers the bottom of the fly inlet tube 131, thereby preventing fruit flies that have entered the operating chamber 13 from escaping from the fly inlet tube 131. After the fruit flies requiring anesthesia are introduced into the operating chamber 13 through the fruit fly culture bottle, a small amount of ether is adsorbed by a dropper and dripped into the operating chamber 13 through the anesthesia tube 132. The ether enters the operating chamber 13 through the anesthesia tube 132, thereby anesthetizing the fruit flies in the operating chamber 13. After the ether is dripped into the anesthesia tube 132, the anesthesia tube 132 is sealed with the second tube cap 135 to prevent the ether from overflowing from the anesthesia tube 132. If it is necessary to increase the dosage of ether during the process, the second tube cap 135 is opened and ether is dripped into the anesthesia tube 132. Through the transparent box 1, when all the fruit flies in the operating chamber 13 are anesthetized, the experimenter uses the left inner glove 14 and the right inner glove 15 to push the anesthetized fruit flies in the operating chamber 13 to the guide slope 19 of the base plate 18. The anesthetized fruit flies are pushed into the fly outlet tube 133 through the guide slope 19. The fly outlet tube 133 is then inserted into the fruit fly culture bottle, thereby transferring the anesthetized fruit flies into the fruit fly culture bottle.After all fruit flies have been removed from the operating chamber 13, the perforation shield 112 on the partition 11 is removed using the left inner glove 14 and the right inner glove 15, connecting the purification chamber 12 and the operating chamber 13. This allows the absorption box 3 within the purification chamber 12 to absorb the ether in the operating chamber 13. Once the ether in the operating chamber 13 has been largely absorbed, the perforation shield 112 can be used again to cover the first absorption hole 111, disconnecting the purification chamber 12 from the operating chamber 13. After the experiment is completed, the front cover 17 can be opened to clean and disinfect the inside of the chamber 1.

Claims

1. An experimental device for fruit fly anesthesia, characterized by, Including transparent box (1), the box (1) inside is provided with partition (11), the partition (11) will the inside of box (1) divide into purification cavity (12) and operation cavity (13), be provided with several first absorption hole (111) on the partition (11), be provided with detachable hole cover plate (112) on the partition (11), the hole cover plate (112) with partition (11) magnetically connected; The left side and the right side of the box (1) are provided with operation openings, and the operation openings are sealingly connected with the built-in gloves. The top of the purification cavity (12) is provided with a strip-shaped opening (121), and the strip-shaped opening (121) is provided with a detachable absorption box (3). The top of the operation cavity (13) is provided with an inlet tube (131) and an anesthesia tube (132), and the bottom of the operation cavity (13) is provided with an outlet tube (133). The diameter of the inlet tube (131) is the same as that of the outlet tube (133). The inlet tube (131) is further provided with a culture bottle access mechanism (2). The culture bottle access mechanism (2) comprises a lower limit ring (21), an upper movable ring (22), a spring (23), a one-word-shaped support (24), a support rod (25) and an umbrella-shaped on-off part (26). The lower limit ring (21) is fixedly connected with the inlet tube (131). The bottom of the spring (23) is fixedly connected with the lower limit ring (21). The top of the spring (23) is fixedly connected with the upper movable ring (22). The upper movable ring (22) slides in the inlet tube (131). The one-word-shaped support (24) is fixed in the upper movable ring (22). The top of the support rod (25) is fixedly connected with the one-word-shaped support (24). The bottom of the support rod (25) is fixedly connected with the umbrella-shaped on-off part (26). When the spring (23) is in a normal state, the umbrella-shaped on-off part (26) communicates the inlet tube (131) with the purification cavity (12). The hole cover plate (112) is an iron plate. The hole cover plate (112) is provided with a rubber layer on the side abutting against the first absorption hole (111). Four magnets are uniformly arranged outside the first absorption hole (111). The magnets are inlaid on the partition (11). The hole cover plate (112) is adsorbed on the partition (11) by the magnets to shield the first absorption hole (111). The absorption box (3) comprises an absorption box body (31) and a cover body (32). The top of the absorption box body (31) is provided with a cover body opening matched with the cover body (32). The cover body (32) is inserted and fixed with the absorption box body (31) through the cover body opening. The cover body (32) is sealingly connected with the cover body opening. The front and rear sides of the absorption box body (31) are provided with a plurality of second absorption holes (33).

2. The experimental set-up for Drosophila anesthesia according to claim 1, characterized in that, The umbrella-shaped on-off part (26) is made of rubber. The projection area of the umbrella-shaped on-off part (26) in the top view is greater than the projection area of the inlet tube (131).

3. The experimental set-up for Drosophila anesthesia according to claim 1, characterized in that, The sealing rubber ring (34) is sealingly connected with the strip-shaped opening (121) and the absorption box (3).

4. The experimental apparatus for Drosophila anesthesia according to claim 1, wherein The first pipe cap (134) is provided on the fly inlet pipe (131) and is threadedly connected with the fly inlet pipe (131); the second pipe cap (135) is provided on the anesthesia pipe (132) and is threadedly connected with the anesthesia pipe (132); the third pipe cap (136) is provided on the fly outlet pipe (133) and is threadedly connected with the fly outlet pipe (133), and the inner top of each of the first pipe cap (134), the second pipe cap (135) and the third pipe cap (136) is provided with a sealing rubber pad.

5. The experimental set-up for Drosophila anesthesia according to claim 1, characterized in that, The absorption box (3) is provided with an absorbent, and the absorbent is activated carbon.

6. The experimental set-up for Drosophila anesthesia according to claim 1, characterized in that, The bottom of the box body (1) is provided with a bottom plate (18), the bottom plate (18) is provided with a guide inclined surface (19) on the side close to the fly outlet pipe (133), the fly outlet pipe (133) is obliquely arranged downward, and the bottom of the guide inclined surface (19) is connected with the inlet side of the fly outlet pipe (133).

7. The experimental set-up for Drosophila anesthesia according to claim 1, characterized by, The front side of the box body (1) is provided with a circular front side opening (16), the front side opening (16) is provided with a front hatch (17), the front side opening (16) is sealingly connected with the front hatch (17), one end of the front hatch (17) is hingedly connected with the box body (1), and the outer portion of the front hatch (17) is provided with a rubber sealing ring matched with the front side opening (16).

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