A composition for improving sports myocardial injury

Abstract

The invention discloses a composition for improving sports myocardial injury, which mainly comprises: soybean peptide powder, branched-chain amino acids, taurine, L-arginine, glutamine, medium-chain triglycerides, maltooligosaccharides, glucose, edible flavors and vitamins. The composition is prepared by mixing a plurality of different raw materials in different proportions, can significantly reduce the concentrations of myocardial troponin T, serum creatine kinase and superoxide dismutase after high-load exercise, and improves myocardial cell damage, with significant effects, and can be used as a protective agent for sports myocardial injury.

Description

Technical Field

[0001] The invention relates to the field of health, and in particular to a composition for improving exercise-induced myocardial injury. Background Art

[0002] Athletes and military operators usually face high-intensity exercise loads. Continuous high-intensity loads usually bring a series of adverse effects on human organs, among which the heart is the most affected, usually damaging the heart's tissue structure and causing myocardial damage, thereby affecting heart function, leading to serious arrhythmias, heart failure, decreased exercise capacity, and even increased risk of death. In recent years, research on how to effectively improve myocardial damage caused by various reasons has been in the ascendant, and a variety of treatment methods have emerged, such as drug intervention, supersaturated oxygen therapy, and ultra-low temperature therapy, but these methods can be truly effectively applied in clinical practice.

[0003] Chinese invention patent CN113230356A discloses the use of kudzu root and celery soup in the preparation of an anti-septicemia and myocardial injury composition, which uses kudzu root and celery as the main therapeutic substances, supplemented by hawthorn, cassia seed, yam, wolfberry, corn silk, and oligofructose to effectively improve myocardial tissue damage caused by sepsis;

[0004] CN101766590A discloses a composition for protecting myocardial peroxidation damage. The composition includes coenzyme Q, L-carnitine, and octacosanol, which can reduce lipid peroxidation reaction of myocardial cell membrane and increase the activity of antioxidant enzymes SOD, GSH-Px, and CAT, thereby protecting myocardial peroxidation damage and delaying or eliminating the occurrence of sports fatigue.

[0005] The above-mentioned existing technologies can improve the condition of myocardial tissue damage, but currently people tend to seek a quick and convenient way to improve sports myocardial damage, and there is still a need to propose new compositions that can help improve sports myocardial damage. Summary of the invention

[0006] In view of the deficiencies of the prior art, the present invention aims to provide a composition for improving sports myocardial injury, which is mainly used in high-load exercise groups, especially military operators before preparing for long-term, high-intensity exercise. By simply taking the composition orally, the repair rate in the body can be accelerated, the activity of superoxide dismutase SOD can be increased, and the increase of troponin T can be inhibited, thereby protecting the myocardium from peroxidation damage.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] The invention provides a composition for improving sports myocardial injury, comprising the following raw materials in parts by weight: 15-25 parts of soybean peptide powder, 10-15 parts of branched-chain amino acids, 8-16 parts of taurine, 15-25 parts of L-arginine, 20-25 parts of glutamine, 10-20 parts of medium-chain triglycerides and 35-40 parts of auxiliary materials.

[0009] The auxiliary materials include 10-25 parts of maltooligosaccharide, 10-25 parts of glucose, 2-6 parts of edible flavors, and 6-18 parts of vitamins, wherein the vitamins include 6-10 parts of vitamin B1, 2-4 parts of vitamin B2, and 2-4 parts of vitamin B6.

[0010] As a further preferred embodiment of the present invention, the composition for improving sports myocardial injury comprises the following raw materials in parts by weight: 25 parts of soybean peptide powder, 15 parts of branched-chain amino acids, 10 parts of taurine, 20 parts of L-arginine, 25 parts of glutamine, 20 parts of medium-chain triglycerides, and 35 parts of auxiliary materials.

[0011] The auxiliary materials include 25 parts of maltooligosaccharide, 25 parts of glucose, 6 parts of edible flavors, and 18 parts of vitamins, wherein the vitamins include 8 parts of vitamin B1, 2 parts of vitamin B2, and 2 parts of vitamin B6.

[0012] Furthermore, the myocardial injury refers to myocardial disorder, muscle fiber swelling, and myocardial cell degeneration and necrosis caused by high exercise load.

[0013] Furthermore, the preparation method of soybean peptide powder is as follows: soybean protein powder and deionized water are mixed in a weight ratio of 1:10, heated to 90°C for 30 minutes, the pH is adjusted to 7.0, and when the temperature drops to 50°C, 2% of the complex enzyme solution of alkaline protease and neutral protease is added, and enzymolysis is carried out at a constant temperature for 5 hours. After the reaction is completed, the reaction is filtered, the filtrate is vacuum concentrated, and finally the soybean peptide powder is obtained by spray drying.

[0014] Furthermore, the composition for improving exercise-induced myocardial injury is in the form of capsules, tablets, pills, powders, oral liquids, granules or beverages.

[0015] Furthermore, the above components are weighed according to the proportions, mixed and stirred evenly, and taken by the operator before high-intensity exercise.

[0016] The glutamine used in the present invention can provide the body with an essential nitrogen source, promote protein synthesis in muscle cells, promote the growth and differentiation of muscle cells through cell volume expansion, and stimulate the secretion of growth hormone, insulin and testosterone, so that the body is in a synthetic state.

[0017] The arginine used in the present invention can effectively improve immunity, promote the immune system to secrete endogenous substances such as natural killer cells, phagocytes, and interleukin-1, which is beneficial for fighting cancer cells and preventing viral infections. In addition, arginine is a precursor of ornithine (L-ornithine) and proline (L-proline), and proline is an important element in the formation of collagen. Supplementing arginine is significantly helpful for the care of severe trauma, burns, etc. that require a lot of tissue repair, and it also has the effect of reducing infection and inflammation.

[0018] Vitamin B1 used in the present invention, also known as thiamine, is the earliest discovered water-soluble vitamin. It mainly acts as a coenzyme to maintain normal glucose metabolism in the human body, converts glucose into heat, provides energy for the body, has a protective and regulatory effect on the nervous system, improves mental state, regulates water and salt metabolism, maintains heart function, promotes gastrointestinal motility, increases appetite, and improves indigestion.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) The composition is formulated into tablets or capsules with a reasonable ratio of soybean peptide powder, branched-chain amino acids, taurine, L-arginine, glutamine, medium-chain triglycerides, oligomaltose, glucose, edible flavors, vitamin B1, vitamin B2, and vitamin B6, aiming at the characteristics of athletes and military operators who have vigorous metabolism, great physical exertion, and myocardial damage. The composition can effectively improve muscle fiber tissue and protect important tissues such as the heart and muscles of such people.

[0021] (2) The present composition can significantly reduce the levels of serum myocardial injury markers (cardiac troponin T, serum creatine kinase, superoxide dismutase) and alleviate the level of oxidative stress to achieve the purpose of protecting the myocardium. In pathological examinations, the protective effects of low, medium and high doses of the composition on the exercising myocardium can also be observed.

[0022] (3) The various raw materials and auxiliary materials used in the composition are widely available and can be produced by mature methods, making the pharmaceutical composition of the present invention convenient for industrial large-scale production. In addition, by changing the loading mode of the composition, it can be made into capsules, tablets, oral liquids, beverages or other dosage forms, making the composition stable in efficacy and easy to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The effect of the composition on serum cardiac troponin T and serum creatine kinase.

[0024] Figure 2 The effect of the composition on lactate dehydrogenase.

[0025] Figure 3The invention discloses an effect of the composition on the oxidative stress level of myocardial tissue.

[0026] Figure 4 The effect of the composition on myocardial morphology. DETAILED DESCRIPTION

[0027] The technical solution of this patent is further described in detail below in conjunction with the specific implementation methods. It should be pointed out that the following specific implementation methods are detailed descriptions of the preferred implementation methods of this patent, but this patent is not limited to the above implementation methods. Within the knowledge scope of ordinary technicians in this field, various changes can be made without departing from the technical scope of this patent. The following components are calculated by weight unless otherwise specified.

[0028] In the embodiments of the present invention, words such as "exemplary" or "for example" are used to indicate examples, illustrations or explanations. Any embodiment or design described as "exemplary" or "for example" in the embodiments of the present invention should not be interpreted as being more preferred or more advantageous than other embodiments or designs. Specifically, the use of words such as "exemplary" or "for example" is intended to present related concepts in a specific way.

[0029] It should be noted that the mice used in the following examples are male, purchased from Shanghai Bikekeyi Biotechnology Co., Ltd. (animal license number SCXK (Shanghai) 2023-0009), and kept in the animal room of the Navy Special Medical Center. The raw materials and reagents used were purchased from the market. Unless otherwise specified, the experimental methods or related detection methods used in the following examples adopt methods known in the art.

[0030] It should be noted that cardiac troponin T (cTnT), serum creatine kinase (CK), and lactate dehydrogenase (LDH) are markers of myocardial injury, among which troponin is currently the most sensitive and specific biochemical marker for the diagnosis of myocardial injury. Since myocardial cell damage releases troponin, plasma troponin levels can rise as early as 3-4 hours after myocardial injury and can continue to rise for several days; creatine kinase can be detected in the blood after myocardial injury, usually rising within 6 hours after injury, peaking at 12-24 hours, and falling to baseline levels within 24-48 hours.

[0031] Example 1

[0032] Weigh the following raw materials separately: 25g soy peptide powder, 15g branched-chain amino acids, 10g taurine, 20g L-arginine, 25g glutamine, 20g medium-chain triglycerides, 25g maltooligosaccharides, 25g glucose, 6g edible flavor, 8g vitamin B1, 2g vitamin B2, and 2g vitamin B6, and stir well.

[0033] The preparation method of soybean peptide powder is as follows:

[0034] Soy protein powder and deionized water were mixed in a weight ratio of 1:10, heated to 90°C for 30 minutes, the pH was adjusted to 7.0, and when the temperature dropped to 50°C, 2% alkaline protease and neutral protease complex enzyme solution were added, and the enzymatic hydrolysis was carried out at a constant temperature for 5 hours. After the reaction was completed, the filtrate was filtered, and the filtrate was vacuum concentrated. Finally, the soybean peptide powder was obtained by spray drying.

[0035] Example 2

[0036] Weigh the following raw materials respectively: soybean peptide powder 15g, branched-chain amino acids 15g, taurine 16g, L-arginine 15g, glutamine 25g, medium-chain triglycerides 15g, maltooligosaccharides 25g, glucose 25g, edible flavor 6g, vitamin B1 10g, vitamin B2 4g, vitamin B6 4g;

[0037] Wherein, the preparation method of soybean peptide powder is the same as that of Example 1.

[0038] Example 3

[0039] Weigh the following raw materials respectively: 25g soybean peptide powder, 15g branched-chain amino acids, 10g taurine, 25g L-arginine, 25g glutamine, 20g medium-chain triglycerides, 25g maltooligosaccharides, 25g glucose, 6g edible flavor, 6g vitamin B1, 2g vitamin B2, and 2g vitamin B6;

[0040] Wherein, the preparation method of soybean peptide powder is the same as that of Example 1.

[0041] Example 4

[0042] Weigh the following raw materials respectively: 25g soybean peptide powder, 15g branched-chain amino acids, 10g taurine, 15g L-arginine, 25g glutamine, 20g medium-chain triglycerides, 25g maltooligosaccharide, 25g glucose, 6g edible flavor, 10g vitamin B1, 2g vitamin B2, and 2g vitamin B6;

[0043] Wherein, the preparation method of soybean peptide powder is the same as that of Example 1.

[0044] Example 5

[0045] The difference from Example 1 is that the raw material formula contains 10 g of maltooligosaccharide, 10 g of glucose and 2 g of edible flavor.

[0046] Effect test:

[0047] (1) Experimental groups:

[0048] The mice were divided into 5 groups according to body weight, with 10 mice in each group, namely a normal group, a model group, and low-, medium- and high-dose groups of the composition (using the raw material ratio of Example 1), with 5 replicates in each group.

[0049] (2) Experimental methods:

[0050] The experimental mice were first subjected to adaptive feeding, and the experiment was started after 7 days.

[0051] Except for the normal group, all mice were subjected to running adaptation training on the treadmill from 1 to 7 days, with a treadmill speed of 15 m / min and a current stimulation intensity of 4.5 mA for 30 minutes each time. For the formal experiment on days 8 to 28, all mice except the normal group were subjected to exhaustion experiments, first exercising at a speed of 10 m / min for 10 min, then at 15 m / min for 10 min, and then at 20 m / min until exhaustion, with a current stimulation intensity of 4.5 mA.

[0052] Administration time: 1 hour before each training; dosage: high-dose group 3g / kg bw, medium-dose group 1.5g / kgbw, low-dose group 0.75g / kg bw.

[0053] Exhaustion criteria: stimulation and expulsion are ineffective, and after running stops, physical signs are manifested as inability to turn over freely in prone position, rapid breathing, fatigue, slow or even no response to physiological stimulation, etc. On the last day, all animals were killed, and blood was collected from the eye plexus vein of mice to measure biochemical, myocardial enzyme and hematological indicators.

[0054] Heart specimens were obtained from mice and cut along the longitudinal sagittal plane. Half of the heart was fixed, embedded in paraffin, and sliced ​​to observe pathological changes. The other half was homogenized on ice with PBS precooled at 4 degrees, centrifuged at 12000rpm at 4 degrees for 15 minutes, and the supernatant was collected. The total protein concentration was determined by the BCA method. The SOD activity and MDA level in the supernatant were also determined and divided by the total protein concentration for standardization to obtain the relative SOD activity and MDA concentration.

[0055] (3) Experimental results:

[0056] A. Effects of the composition on serum myocardial injury markers (e.g. Figure 1 and Figure 2 ):

[0057] After long-term high-load exercise, the levels of cardiac troponin T (cTnT) and serum creatine kinase (CK) in the mouse model group were significantly increased (P<0.01), while the low-, medium- and high-dose groups of the composition were significantly reduced compared with the model group (P<0.01), and there was no significant difference compared with the normal group, indicating that the composition can significantly inhibit the increase in the levels of troponin T and serum creatine kinase caused by exercise, and has a significant improvement effect on exercise-induced myocardial damage;

[0058] For lactate dehydrogenase (LDH), after long-term high-load exercise, the lactate dehydrogenase content in the mouse model group increased, which was not significantly different from the normal group. The low, medium and high dose groups of the combination decreased compared with the model group, but the difference was not significant.

[0059] B. Effects of the composition on oxidative stress level in myocardial tissue (e.g. Figure 3 ):

[0060] Compared with the normal group, the superoxide dismutase (SOD) level in the myocardial tissue of the mice in the model group was significantly reduced (P<0.01), and the low, medium and high dose groups of the combination could significantly increase the reduced SOD level caused by exercise.

[0061] Compared with the normal group, the malondialdehyde (MDA) level in the myocardial tissue of the mice in the model group was significantly increased (P<0.01), and the low, medium and high dose groups of the combination could significantly reduce the increase in MDA level caused by exercise.

[0062] C. Effects of the composition on myocardial morphology (e.g. Figure 4 ):

[0063] After the experiment, the myocardium of the normal control group had intact structure, neat arrangement, clear striations, and no vascular dilation and inflammatory cell infiltration in the interstitium;

[0064] In the model group, the myocardium was disordered, a large number of muscle fibers were swollen and the structure was blurred, myocardial cell degeneration and necrosis were observed locally, and hemorrhage and a small amount of inflammatory cell infiltration were observed in the interstitium;

[0065] Low-dose group: Some muscle fiber swelling, myocardial hemorrhage, local myocardial inflammatory cell infiltration and mild fibrosis were still observed;

[0066] Myocardial hemorrhage, myocardial fibrosis, and inflammatory cell infiltration were observed in the medium-dose group;

[0067] Except for a small amount of myocardial cell swelling, no other obvious abnormalities were found in the high-dose group.

[0068] According to the above experimental results, the composition can significantly reduce the level of serum myocardial injury markers and alleviate the level of oxidative stress to achieve the purpose of protecting the myocardium. In pathological examinations, the improvement effects of low, medium and high doses of the composition on the exercising myocardium can also be observed.

[0069] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. If these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. Use of a composition in the preparation of a drug for improving exercise myocardial injury, wherein The feature is that the composition comprises the following raw materials in parts by weight: Soy peptide powder 25 parts, branched chain amino acids 15 parts, taurine 10 parts, L-arginine 20 parts 25 parts of glutamine, 20 parts of medium chain triglycerides, 35 parts of auxiliary materials, The auxiliary materials include 25 parts of oligomaltose, 25 parts of glucose, 6 parts of edible flavor, Vitamins 18 parts, including 8 parts of vitamin B1, 2 parts of vitamin B2, Vitamin B6 2 parts; Myocardial injury refers to the disorder of myocardial arrangement and muscle fiber swelling caused by high exercise load. Swelling, myocardial cell degeneration and necrosis; The myocardial injury improving drug prepared by the composition is in the form of capsules, tablets, pills, powders, It is available in the form of tablets, oral liquids, granules or beverages; The myocardial injury improving drug prepared by the composition is taken 1 hour before exercise; The preparation method of the soybean peptide powder is as follows: soybean protein powder and deionized water are mixed according to weight. Mix in a ratio of 1:10, heat to 90℃ for 30 minutes, adjust pH to 7.0, and wait until the temperature drops. When the temperature reaches 50℃, add 2% alkaline protease and neutral protease complex enzyme solution, and keep the enzyme at constant temperature. After the reaction is completed, filter and concentrate the filtrate in vacuum. The soybean peptide powder was obtained by spray drying.

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