Control methods for biological sample storage systems and biological sample storage systems

Abstract

The present application relates to the technical field of biological sample storage, and specifically provides a control method of a biological sample storage system and the biological sample storage system. The present application aims to solve the problem that the existing cryopreservation equipment has safety hazards when a fault occurs. To this end, the biological sample storage system comprises a plurality of storage libraries spaced apart along a linear structure, and two adjacent storage libraries are connected by a connecting channel and can transfer sample boxes to each other. The control method comprises: acquiring the temperature of each storage library in real time to obtain a plurality of actual temperatures; determining whether a storage library has a temperature imbalance fault according to the plurality of actual temperatures; and in the case that a storage library has a temperature imbalance fault, selectively performing an auxiliary cooling operation or a sample transfer operation according to the actual temperature of the storage library having the temperature imbalance fault. The control method of the present application can effectively ensure the safety of the samples and avoid economic losses when a storage library has a temperature imbalance fault.

Description

Technical Field

[0001] This invention relates to the field of sample storage technology, and specifically provides a control method for a biological sample storage system and a biological sample storage system. Background Technology

[0002] In the biomedical industry, automated ultra-low temperature equipment is needed to store biological samples. When the number of samples is large or to mitigate storage risks, multiple automated storage devices are usually required. Each automated storage device is relatively independent. When it is necessary to transfer a sample from one device to another for storage, the sample must first be removed from the first device and then stored in the second device. This transfer process can easily cause sample inactivation.

[0003] Furthermore, when equipment malfunctions and causes changes in the storage environment, it is difficult to transfer samples from the equipment, posing a high security risk.

[0004] Accordingly, a new technical solution is needed in this field to solve the above-mentioned technical problems. Summary of the Invention

[0005] The present invention aims to solve the above-mentioned technical problems, namely, to solve the problem that there are safety hazards to samples when existing cryopreservation equipment malfunctions.

[0006] In a first aspect, the present invention provides a control method for a biological sample storage system, the biological sample storage system comprising multiple storage containers spaced apart along a linear structure, adjacent storage containers being sealed and connected by a connecting channel and capable of transferring sample boxes between them; the control method comprising: acquiring the temperature of each storage container in real time to obtain multiple actual temperatures; determining, based on the multiple actual temperatures, whether any storage container has a temperature imbalance fault; in the case of a storage container having a temperature imbalance fault, selectively performing an auxiliary cooling operation or a sample transfer operation based on the actual temperature of the storage container with the temperature imbalance fault; wherein, the auxiliary cooling operation specifically involves providing liquid nitrogen to the storage container for auxiliary cooling; the sample transfer operation specifically involves transferring a sample stored in a storage container to be transferred to a target storage container, wherein the storage container to be transferred is the storage container with the temperature imbalance fault, and the target storage container is the storage container without the temperature imbalance fault.

[0007] In the preferred embodiment of the control method for the above-mentioned biological sample storage system, the step of "determining whether the storage container has a temperature imbalance fault based on multiple actual temperatures" specifically includes: comparing each actual temperature with a first preset temperature; if the actual temperature is higher than the first preset temperature, then determining that the storage container corresponding to the actual temperature has a temperature imbalance fault; if the actual temperature is not higher than the first preset temperature, then determining that the storage container corresponding to the actual temperature has not a temperature imbalance fault.

[0008] In a preferred embodiment of the control method for the aforementioned biological sample storage system, the step of "selectively performing an auxiliary cooling operation or a sample transfer operation based on the actual temperature of the storage container where the temperature imbalance fault occurs" specifically includes: comparing the actual temperature of the storage container where the temperature imbalance fault occurs with a second preset temperature; if the actual temperature is not higher than the second preset temperature, then performing the auxiliary cooling operation; if the actual temperature is higher than the second preset temperature, then performing the sample transfer operation; wherein, the second preset temperature is higher than the first preset temperature.

[0009] In the preferred embodiment of the control method for the above-mentioned biological sample storage system, the step of "performing auxiliary cooling operation" specifically includes: calculating the difference between the actual temperature and the first preset temperature; determining the amount of liquid nitrogen introduced based on the difference; and introducing liquid nitrogen into the storage chamber where the temperature imbalance fault occurs according to the amount of liquid nitrogen introduced.

[0010] In the preferred embodiment of the control method for the above-mentioned biological sample storage system, the step of "performing a sample transfer operation" specifically includes: obtaining the total number of sample boxes occupied by the samples to be transferred, denoted as the number of sample boxes to be transferred; obtaining the number of empty sample boxes in each target storage repository, resulting in a plurality of empty sample box numbers; adding up all the empty sample box numbers to obtain the total number of empty sample boxes; selectively executing a sample box exchange mode or a sample box exchange combined tube picking mode according to the number of sample boxes to be transferred and the total number of empty sample boxes; wherein, the sample box exchange mode specifically includes exchanging the storage positions of the sample boxes to be transferred with the empty sample boxes; the sample box exchange combined tube picking mode specifically includes exchanging the storage positions of the sample boxes to be transferred containing whole boxes of samples with the empty sample boxes, and storing the sample picking tubes to be transferred from the sample boxes to be transferred that do not contain whole boxes of samples into the empty sample boxes and / or storing the sample picking tubes to be transferred from the sample boxes to be transferred that do not contain whole boxes of samples into the half-storage sample boxes in the target storage repository.

[0011] In the preferred embodiment of the control method for the aforementioned biological sample storage system, the step of "selectively executing a sample box exchange mode or a sample box exchange combined tube picking mode based on the number of sample boxes to be transferred and the total number of empty sample boxes" specifically includes: comparing the number of sample boxes to be transferred with the total number of empty sample boxes; if the number of sample boxes to be transferred is not greater than the total number of empty sample boxes, then executing the sample box exchange mode; if the number of sample boxes to be transferred is greater than the total number of empty sample boxes, then executing the sample box exchange combined tube picking mode.

[0012] In the preferred embodiment of the control method for the above-mentioned biological sample storage system, the step of "executing the sample box exchange mode" specifically includes: determining the number of first target boxes that each target repository needs to receive for the sample boxes to be transferred, based on the number of sample boxes to be transferred, the number of multiple free sample boxes, and the distance relationship between each target repository and the sample box to be transferred; determining the first target free sample box that each target repository needs to exchange positions with the sample boxes to be transferred, based on the number of first target boxes that each target repository needs to receive; matching and binding each sample box to be transferred with one of the first target free sample boxes; and exchanging the storage positions of the bound sample boxes to be transferred and the first target free sample boxes.

[0013] In the preferred embodiment of the control method for the above-mentioned biological sample storage system, the sample box exchange combined tube picking mode includes a sample box exchange operation and a tube picking operation. The steps of "executing the sample box exchange combined tube picking mode" specifically include: executing the sample box exchange operation; and executing the tube picking operation.

[0014] In the preferred embodiment of the control method for the above-mentioned biological sample storage system, the step of "performing the sample box exchange operation" specifically includes: obtaining the number of second sample boxes to be transferred in the sample boxes to be transferred, denoted as the number of second sample boxes to be transferred, wherein the second sample box to be transferred is the sample box to be transferred containing a whole box of sample tubes to be transferred; determining the number of second target boxes that each target repository needs to receive for the second sample boxes to be transferred based on the number of second sample boxes to be transferred, the number of multiple free sample boxes, and the distance relationship between each target repository and the repository to be transferred; determining the number of second target free sample boxes that need to be exchanged with the second sample boxes to be transferred in each target repository based on the number of second target boxes that each target repository needs to receive; matching and binding each second sample box to be transferred with one second target free sample box; and exchanging the storage locations of the bound second sample boxes to be transferred and the second target free sample boxes.

[0015] In the preferred embodiment of the control method for the above-mentioned biological sample storage system, the step of "performing the tube picking operation" specifically includes: obtaining the total number of tubes to be picked and transferred in all the sample boxes to be transferred, denoted as the total number of tubes picked, wherein the tubes to be picked and transferred are the samples to be transferred stored in the third sample box to be transferred, and the third sample box to be transferred is the sample box to be transferred that stores the tubes to be transferred but is not full; obtaining again the number of empty sample boxes in each target storage area to obtain multiple empty sample boxes for tube picking; and determining the multiple empty sample boxes for tube picking and the storage capacity of each sample box according to the storage capacity of each sample box. The quantity is determined by selecting the number of samples to be picked. Based on the number of samples to be picked and the total number of samples to be picked, a first pairing picking operation or a second pairing picking operation is selectively performed. Specifically, the first pairing picking operation includes picking all the samples to be picked and transferred into the empty sample box. Specifically, the second pairing picking operation includes dividing all the samples to be picked and transferred into a first picking part and a second picking part. First, the samples to be picked and transferred from the first picking part are picked into the empty sample box. Then, the samples to be picked and transferred from the second picking part are picked into the semi-storage sample box in the target storage repository.

[0016] In the preferred embodiment of the control method for the above-mentioned biological sample storage system, the step of "selectively performing a first paired tube picking operation or a second paired tube picking operation according to the tube receiving quantity and the total number of tubes" specifically includes: comparing the tube receiving quantity with the total number of tubes; if the tube receiving quantity is not less than the total number of tubes, then performing the first paired tube picking operation; if the tube receiving quantity is less than the total number of tubes, then performing the second paired tube picking operation.

[0017] In a preferred embodiment of the control method for the above-mentioned biological sample storage system, during the execution of the second pairing tube picking operation, the control method further includes: obtaining the total number of samples to be transferred in the second tube picking section, denoted as the second total tube picking amount; obtaining the total number of semi-storage sample boxes received in all the target storage containers, denoted as the second tube picking received amount; and selectively issuing an emergency alarm based on the second total tube picking amount and the second tube picking received amount.

[0018] In a second aspect, the present invention provides a biological sample storage system, the biological sample storage system including a controller configured to execute the control method of the biological sample storage system described above.

[0019] When the above technical solution is adopted, the control method of the present invention determines whether there is a temperature imbalance fault in the storage warehouse based on multiple actual temperatures. If a temperature imbalance fault occurs in the storage warehouse, auxiliary cooling operation or sample transfer operation is selectively executed according to the actual temperature of the storage warehouse with the temperature imbalance fault. By setting it up in this way, auxiliary cooling operation or sample transfer operation is executed according to the actual temperature, so as to cool down the storage warehouse in time or transfer the sample to a safe environment, effectively ensuring the safety of the sample and avoiding economic losses when a temperature imbalance fault occurs in the storage warehouse.

[0020] Furthermore, the step of "determining whether a storage unit has a temperature imbalance fault based on multiple actual temperatures" specifically includes: comparing each actual temperature with a first preset temperature; if the actual temperature is higher than the first preset temperature, it is determined that the storage unit corresponding to that actual temperature has a temperature imbalance fault; if the actual temperature is not higher than the first preset temperature, it is determined that the storage unit corresponding to that actual temperature has not a temperature imbalance fault. This setting method is simple and fast.

[0021] Furthermore, the step of "selectively performing auxiliary cooling operations or sample transfer operations based on the actual temperature of the storage unit where the temperature imbalance fault has occurred" specifically includes: comparing the actual temperature of the storage unit where the temperature imbalance fault has occurred with a second preset temperature; if the actual temperature is not higher than the second preset temperature, then performing auxiliary cooling operations; if the actual temperature is higher than the second preset temperature, then performing sample transfer operations. With this setting method, the judgment method is simple, the response is fast, and it is convenient to control.

[0022] Furthermore, based on the number of sample boxes to be transferred and the total number of available sample boxes, a sample box exchange mode or a sample box exchange combined tube picking mode can be selectively executed. This allows for the selection of the fastest transfer scheme based on the storage conditions in the storage repository where temperature imbalance failure has occurred, thereby saving transfer time and ensuring the safety of most samples.

[0023] Furthermore, the steps of "executing the sample box exchange mode" specifically include: determining the number of first target boxes that each target repository needs to receive based on the number of sample boxes to be transferred, the number of multiple spare sample boxes, and the distance relationship between each target repository and the repository to be transferred; determining the first target spare sample box that each target repository needs to exchange positions with the sample boxes to be transferred based on the number of first target boxes that each target repository needs to receive; matching and binding each sample box to be transferred with one first target spare sample box; and exchanging the storage locations of the bound sample boxes to be transferred and the first target spare sample boxes. This setting prioritizes transferring to intact repositories that are closer to the repository that experienced the temperature imbalance failure, thereby saving transfer time.

[0024] Furthermore, the sample box exchange combined tube picking mode includes a sample box exchange operation and a tube picking operation. The specific steps of "executing the sample box exchange combined tube picking mode" include: performing the sample box exchange operation; performing the tube picking operation. This setting method, which performs the sample box exchange operation first and then the tube picking operation, can save the time required for sample transfer and ensure sample safety to the greatest extent.

[0025] Furthermore, during the tube picking operation, the first or second pairing tube picking operation is selectively performed based on the number of tubes received and the total number of tubes picked. This setting method determines the appropriate tube picking mode for transfer based on the total number of tubes picked and the number of tubes received, thereby saving the time required for transfer and ensuring sample safety.

[0026] Furthermore, during the second pairing tube picking operation, an emergency alarm is selectively triggered based on the total number of tubes picked and the number of tubes received. When the total number of tubes picked exceeds the number of tubes received, an alarm is triggered to remind the user to transfer excess samples in a timely manner and minimize losses. Attached Figure Description

[0027] The preferred embodiments of the present invention are described below with reference to the accompanying drawings, in which:

[0028] Figure 1 This is a schematic diagram of the biological sample storage system of the present invention;

[0029] Figure 2 This is a flowchart of the main steps of the control method for the biological sample storage system of the present invention;

[0030] Figure 3 This is a flowchart of an embodiment of the control method for the biological sample storage system of the present invention;

[0031] Figure 4 This is a flowchart illustrating the specific execution steps of the control method for the biological sample storage system of the present invention, specifically the step of "performing a sample transfer operation".

[0032] Figure 5 This is a flowchart illustrating the specific execution steps of the "executing sample box exchange mode" step in the control method of the biological sample storage system of the present invention.

[0033] Figure 6 This is a flowchart illustrating the specific execution steps of the control method for the biological sample storage system of the present invention, specifically the step of "executing the sample and exchange combined tube picking mode".

[0034] Figure 7This is a flowchart illustrating the specific execution steps of the control method for the biological sample storage system of the present invention, specifically the step of "performing a sample box exchange operation".

[0035] Figure 8 This is a flowchart illustrating the specific execution steps of the "pipe picking operation" step in the control method of the biological sample storage system of the present invention.

[0036] List of reference numerals in the attached diagram:

[0037] 1. Repository; 11. Cache area; 12. Storage area;

[0038] 2. Connecting channel; 3. Pipe picking equipment; 4. Storage rack; 5. Frozen storage area transfer robot; 6. First conveying mechanism; 7. Second conveying mechanism; 8. Transmission mechanism. Detailed Implementation

[0039] Preferred embodiments of the present invention will now be described with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are merely illustrative of the technical principles of the present invention and are not intended to limit the scope of protection of the present invention.

[0040] It should be noted that in the description of this invention, terms such as "inner" and "outer" indicating directional or positional relationships are based on the directional or positional relationships shown in the accompanying drawings. This is merely for ease of description and does not indicate or imply that the device or element must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0041] Furthermore, it should be noted that, in the description of this invention, unless otherwise explicitly specified and limited, the terms "set" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through other components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0042] Based on the issue of sample safety risks associated with existing storage devices malfunctioning as mentioned in the background section, the control method of this invention, in the event of a temperature imbalance failure in a storage storage unit, selectively performs auxiliary cooling operations or sample transfer operations according to the actual temperature of the storage unit experiencing the temperature imbalance failure. This allows for timely remedial action by cooling the storage unit or transferring the samples to a safe environment, effectively ensuring sample safety and preventing economic losses when a storage unit experiences a temperature imbalance failure.

[0043] Specifically, please refer to Figure 1The biological sample storage system of the present invention includes multiple storage containers 1, which are distributed at intervals along a linear structure. Adjacent storage containers 1 are sealed and connected by a connecting channel 2, and sample boxes can be transferred between adjacent storage containers 1. Each storage container 1 is provided with an entrance / exit that connects to the external environment, enabling the transfer of sample boxes between the storage container 1 and the outside world. Each storage container 1 is equipped with an electric door to seal and open the entrance / exit, preventing the storage container 1 from connecting to the outside world when sealed.

[0044] In practical applications, the linear structure can be a straight line, a curved line, or a broken line. There are no restrictions on the shape of the linear structure. Those skilled in the art can set the shape of the linear structure according to actual needs, as long as the adjacent two storage boxes 1 are sealed through the connecting channel 2 along the extension direction of the linear structure and can transfer sample boxes to each other.

[0045] Preferably, please continue reading. Figure 1 Each storage repository 1 includes an independent storage area 12 and a buffer area 11. Samples are stored in storage area 12. Storage areas 12 and buffer areas 11 within the same storage repository 1 can transfer sample boxes between each other. Specifically, storage areas 12 and buffer areas 11 are connected via a second electric door, allowing selective connection of storage areas 12 and buffer areas 11 for sample box transfer. A connecting channel 2 connects the buffer areas 11 of two adjacent storage repositories 1, enabling the transfer of sample boxes between the buffer areas 11 of adjacent storage repositories 1. Specifically, the storage temperature in storage area 12 is -80°C, and the storage temperature in buffer area 11 is -25°C.

[0046] Connection channel 2 connects the buffer areas 11 of two adjacent storage tanks 1, so that both connection channel 2 and buffer area 11 are at -25°C. When the sample box is transferred through connection channel 2, the sample box can be kept at -25°C, effectively ensuring the safety of the sample.

[0047] Preferably, please continue reading. Figure 1 The biological sample storage system of the present invention also includes a transmission mechanism 8, which passes through the connecting channel 2 and connects multiple buffer areas 11. The transmission mechanism 8 is configured to transmit sample boxes along its length direction, thereby enabling the transmission of sample boxes within multiple buffer areas 11 and thus realizing the transfer of sample boxes between different storage storage areas 1.

[0048] Specifically, the transmission mechanism 8 includes a carrier stage that moves along the linear structural direction and a drive mechanism that drives the carrier stage to move along the linear structural direction. The carrier stage can fix and support the sample box.

[0049] Preferably, please continue reading. Figure 1Each storage unit 1 is equipped with a storage rack 4, a cryopreservation area transfer robot 5, a first conveying mechanism 6, and a second conveying mechanism 7. The storage rack 4 is located in the storage area 12 and is used to store sample boxes. The cryopreservation area transfer robot 5 and the first conveying mechanism 6 are located in the storage area 12. The first conveying mechanism 6 is configured to transfer sample boxes between the storage area 12 and the buffer area 11. Specifically, the first conveying mechanism 6 can dock with the cryopreservation area transfer robot 5 to transfer sample boxes, and the first conveying mechanism 6 can also dock with the transmission mechanism 8 to transfer sample boxes. The cryopreservation area transfer robot 5 is configured to transfer sample boxes between the storage rack 4 and the first conveying mechanism 6. The second conveying mechanism 7 is configured to transfer sample boxes between the buffer area 11 and the outside. Specifically, the second conveying mechanism 7 can dock with the transmission mechanism 8 to transfer sample boxes, and the second conveying mechanism 7 can also extend out of the storage unit through an inlet / outlet to transfer sample boxes.

[0050] Specifically, the first conveying mechanism 6 is a first linear conveying mechanism with a tray platform, which can drive the tray platform to move in a straight line; the second conveying mechanism 7 is a second linear conveying mechanism with a shovel mechanism, which can drive the shovel mechanism to move in a straight line, and the shovel mechanism can rotate to receive and transfer sample boxes. The structures of the linear conveying mechanism and the shovel mechanism can adopt conventional structures in this field, and their structures will not be described in detail here.

[0051] Preferably, the biological sample storage system of the present invention further includes at least one tube-picking device 3, which is disposed within the buffer area 11 of one of the storage containers 1. More preferably, the tube-picking device 3 is disposed within the buffer area 11 of one of the storage containers 1 located at the end of the linear structure, which effectively saves space and facilitates assembly and use. The tube-picking device 3 can adopt a conventional structure in the art, and its structure will not be described in detail here.

[0052] Preferably, each storage repository 1 has a barcode scanner in its buffer area 11 to scan sample boxes and cryopreservation tubes during entry and exit. This barcode scanner can be linked to the first conveying mechanism 6 or the second conveying mechanism 7 to perform the scanning operation during sample box transfer, or it can be a standalone device. The barcode scanner can use a conventional structure in the art, and its structure will not be described in detail here. Alternatively, a single barcode scanner can be provided, linked to the transmission mechanism 8 to perform the scanning operation during transmission, and one scanner can serve multiple storage repositories 1 simultaneously, effectively saving costs.

[0053] Furthermore, the biological sample storage system of the present invention also includes a controller capable of executing the control method of the biological sample storage system of the present invention. The controller may be a single unit that simultaneously controls the operation of devices within multiple storage repositories 1 and manages information within multiple storage repositories 1; alternatively, multiple controllers may be configured and interconnected to exchange information, with each controller controlling the operation of devices within one storage repositories 1 and managing information within its corresponding storage repositories 1.

[0054] Furthermore, based on the structure of the aforementioned biological sample storage system, this invention also provides a control method for the biological sample storage system, so as to promptly remedy the situation in the event of a temperature imbalance failure in a storage cell, and transfer the sample to another storage cell when the temperature drop is severe, thereby effectively ensuring the safety of the sample and improving the safety performance of the entire biological sample storage system.

[0055] Specifically, please refer to Figure 2 The control method of the present invention includes the following steps:

[0056] S1: Get the temperature of each repository in real time to obtain multiple actual temperatures.

[0057] Specifically, each storage unit is equipped with a temperature sensor, which is located within the storage area to detect the temperature within the storage area.

[0058] S2: Based on multiple actual temperatures, determine if there is a temperature imbalance fault in the storage warehouse.

[0059] Based on the actual temperature of each storage unit, determine whether the storage unit has experienced a temperature imbalance failure. If no storage unit has experienced a temperature imbalance failure, return to step S1. If a storage unit has experienced a temperature imbalance failure, proceed to step S3.

[0060] S3: In the event of a temperature imbalance failure in a storage unit, selectively perform auxiliary cooling operations or sample transfer operations based on the actual temperature of the storage unit experiencing the temperature imbalance failure.

[0061] The auxiliary cooling operation specifically involves supplying liquid nitrogen into the storage warehouse to maintain the temperature within the warehouse's storage area within its set range. This ensures sample safety and prevents damage due to temperature fluctuations. Simultaneously, during the liquid nitrogen supply process, the system can issue an alarm to remind the user to promptly investigate the cause of the malfunction and carry out repairs, enabling the cooling system of the storage warehouse experiencing temperature imbalance to resume operation in a timely manner.

[0062] The sample transfer operation specifically involves transferring samples stored in a storage repository to a target storage repository. The storage repository to be transferred is one experiencing a temperature imbalance malfunction, while the target storage repository is one that is not experiencing a temperature imbalance malfunction and is therefore a safe storage repository. When the backup liquid nitrogen is depleted and the actual temperature is too high, the sample transfer operation is performed to move the sample device from the temperature imbalance malfunctioning storage repository to a safe storage repository, thus preventing sample deactivation and ensuring sample safety.

[0063] The control method of the present invention performs auxiliary cooling operations or sample transfer operations according to the actual temperature, which can provide timely remedial measures to cool down the storage warehouse or transfer the samples to a safe environment, effectively ensuring sample safety and avoiding economic losses when a storage warehouse experiences temperature imbalance failure.

[0064] Preferably, please refer to Figure 3 Step S2, "Determine whether there is a temperature imbalance fault in the storage warehouse based on multiple actual temperatures," specifically includes:

[0065] S21: Compare each actual temperature with the first preset temperature.

[0066] It determines whether the actual temperature of each storage unit is higher than the first preset temperature, and then, based on the comparison results, determines whether the storage unit corresponding to each actual temperature has experienced a temperature imbalance fault.

[0067] Specifically, this invention does not impose any limitations on the first preset temperature. The first preset temperature can be slightly higher than the target storage temperature of the storage warehouse and within the adjustment range of the refrigeration system of the storage warehouse. For example, if the target storage temperature of the storage warehouse is -80°C and the adjustment range of the refrigeration system of the storage warehouse is ±5°C, then the first preset temperature can be set to -75°C. Adjustments to the specific value of the first preset temperature do not depart from the basic principles of this invention and should be limited to the protection scope of this invention.

[0068] Specifically, the step of "determining whether the storage unit corresponding to each actual temperature has a temperature imbalance fault based on the comparison results" includes steps S22 and S23.

[0069] S22: If the actual temperature is not higher than the first preset temperature, it is determined that the storage unit corresponding to the actual temperature has not experienced a temperature imbalance fault.

[0070] S23: If the actual temperature is higher than the first preset temperature, it is determined that the storage unit corresponding to the actual temperature has a temperature imbalance fault.

[0071] The determination method of this preferred embodiment is simple, easy to execute, and has a fast response speed.

[0072] It should be noted that in practical applications, step S2 can also be set as other execution steps. For example, the judgment can be made directly based on the actual temperature. If the actual temperature is within a first preset range, it is determined that the storage unit corresponding to that actual temperature has no temperature imbalance fault; if the actual temperature is within a second preset range, it is determined that the storage unit corresponding to that actual temperature has a temperature imbalance fault. Alternatively, the difference between the actual temperature and the first preset temperature can be calculated, and the magnitude of the difference can be used to determine whether the storage unit corresponding to the actual temperature has a temperature imbalance fault. And so on. Such adjustments and changes to the specific execution steps of step S2 do not deviate from the basic principles of the present invention and should all be limited to the protection scope of the present invention.

[0073] Preferably, please refer to Figure 3 Step S3, "In the event of a temperature imbalance failure in a storage warehouse, selectively perform auxiliary cooling operations or sample transfer operations based on the actual temperature of the storage warehouse experiencing the temperature imbalance failure," specifically includes:

[0074] S31: Compare the actual temperature of the storage unit where the temperature imbalance fault occurred with a second preset temperature. The second preset temperature is higher than the first preset temperature.

[0075] Determine whether the actual temperature of the storage unit with the temperature imbalance fault is higher than a second preset temperature, and then selectively perform auxiliary cooling operations or sample transfer operations based on the comparison results.

[0076] Specifically, this invention does not impose any restrictions on the specific value of the second preset temperature, as long as the second preset temperature is higher than the first preset temperature. For example, the first preset temperature is set to -75℃, and the second preset temperature can be set to -60℃. Adjustments to the specific value of the second preset temperature do not deviate from the basic principles of this invention and should be limited to the scope of protection of this invention.

[0077] Specifically, the step of "selectively performing auxiliary cooling operations or sample transfer operations based on the comparison results" includes steps S32 and S33.

[0078] S32: If the actual temperature is higher than the second preset temperature, perform a sample transfer operation.

[0079] S33: If the actual temperature is not higher than the second preset temperature, then perform auxiliary cooling operation.

[0080] The determination method of this preferred embodiment is simple, easy to execute, and has a fast response speed.

[0081] It should be noted that in practical applications, step S3 can also be set as other execution steps. For example, the judgment can be made directly based on the actual temperature. When the actual temperature is within the third preset range, an auxiliary cooling operation is performed; when the actual temperature is within the fourth preset range, a sample transfer operation is performed. Alternatively, the difference between the actual temperature and the second preset temperature can be calculated, and the auxiliary cooling operation or the sample transfer operation can be selectively performed based on the magnitude of the difference. Such adjustments and changes to the specific execution steps of step S3 do not deviate from the basic principles of this invention and should all be limited to the protection scope of this invention.

[0082] It should be noted that this invention does not impose any restrictions on the specific execution steps of "performing sample transfer operations". As long as the samples in the storage container with temperature imbalance failure are transferred to a safe storage container, it is acceptable. In practical applications, samples can be transferred by picking tubes or by swapping sample boxes in two storage containers. Any adjustments and changes to the specific transfer process of the sample transfer operation do not deviate from the basic principles of this invention and should be limited to the protection scope of this invention.

[0083] Preferably, please refer to Figure 4 The specific steps for "performing a sample transfer operation" include:

[0084] S321: Obtain the total number of sample boxes occupied by the sample to be transferred, denoted as the number of sample boxes to be transferred.

[0085] Among them, the sample to be transferred refers to the sample stored in the transfer repository. Any sample box in the transfer repository that stores the sample to be transferred is a sample box to be transferred. The sample boxes to be transferred include sample boxes that store a whole box of samples and sample boxes that store a small number of samples (at least one sample).

[0086] S322: Obtain the number of free sample boxes in each target repository to get multiple free sample box counts.

[0087] Among them, the empty sample boxes are the sample boxes that do not store samples in the target storage repository.

[0088] S323: Add up the number of all empty sample boxes to get the total number of empty sample boxes.

[0089] S324: Based on the number of sample boxes to be transferred and the total number of available sample boxes, selectively execute the sample box exchange mode or the sample box exchange combined tube picking mode.

[0090] Specifically, the sample box exchange mode includes exchanging the storage locations of the sample boxes to be transferred with the empty sample boxes.

[0091] The sample box exchange and sample picking mode specifically includes: exchanging the storage locations of sample boxes containing complete boxes of samples with empty sample boxes; storing sample picking tubes from sample boxes not containing complete boxes of samples into empty sample boxes; and storing sample picking tubes from sample boxes not containing complete boxes of samples into half-stored sample boxes in the target storage repository. Here, a half-stored sample box is a sample box in the target storage repository that is not full of complete boxes of samples.

[0092] This setup allows for the selection of the fastest transfer method based on the sample box storage situation in the repository to be transferred, thereby saving transfer time and ensuring the safety of most samples.

[0093] Preferably, please continue reading. Figure 4 Step S324, "Selectively execute the sample box exchange mode or the sample box exchange combined tube picking mode based on the number of sample boxes to be transferred and the total number of empty sample boxes," specifically includes:

[0094] S3241: Determine whether the number of sample boxes to be transferred is greater than the total number of empty sample boxes.

[0095] The number of sample boxes to be transferred is compared with the total number of available sample boxes so that, based on the comparison results, either the sample box exchange mode or the sample box exchange combined pick-up mode can be selectively executed.

[0096] Specifically, the step of "selectively executing the sample box exchange mode or the sample box exchange combined tube picking mode based on the comparison results" includes steps S3242 and S3243.

[0097] S3242: If the number of sample boxes to be transferred is not greater than the total number of empty sample boxes, then execute the sample box exchange mode.

[0098] If the number of sample boxes to be transferred is not greater than the total number of free sample boxes, it means that all sample boxes to be transferred can be transferred to a safe repository, and then the sample box exchange mode is executed.

[0099] S3243: If the number of sample boxes to be transferred is greater than the total number of empty sample boxes, then execute the sample box exchange combined tube picking mode.

[0100] If the number of sample boxes to be transferred is greater than the total number of available sample boxes, it means that not all sample boxes to be transferred can be transferred to a safe repository. In this case, the sample box exchange composite pick-and-roll mode is executed.

[0101] This setup simplifies the comparison process, allows for faster determination of specific transfer plans, facilitates application, and saves time.

[0102] Preferably, please refer to Figure 5The specific steps for “executing the sample box exchange mode” include:

[0103] S32421: Based on the number of sample boxes to be transferred, the number of multiple spare sample boxes, and the distance relationship between each target repository and the repository to be transferred, determine the first target box number of sample boxes to be transferred that each target repository needs to receive.

[0104] Specifically, the system prioritizes selecting the target repository closest to the repository to be transferred, and only selects the next closest target repository after the nearest target repository can no longer receive the data. This setting can save the time required for the transfer of sample boxes during the transfer process, thereby improving the transfer efficiency.

[0105] S32422: Based on the number of first target boxes that each target repository needs to receive, determine the first target spare sample box that each target repository needs to swap positions with the sample box to be transferred.

[0106] S32423: Match and bind each sample box to be transferred with a first target empty sample box.

[0107] Specifically, binding can be done based on the location information of each sample box in the original repository, or it can be done based on the box code information of each sample box.

[0108] S32424: Swap the storage locations of the bound sample box to be transferred and the first target empty sample box.

[0109] In addition, during the exchange process, both sample boxes are scanned to determine if the exchange is successful, ensuring the accuracy of the exchange and timely updating of management information within the control system.

[0110] This setup binds each sample box to be transferred to a first target empty sample box and allows for their interchange, enabling orderly transfer and facilitating control and updating of stored information.

[0111] Preferably, please refer to Figure 6 The sample box exchange and tube picking mode includes sample box exchange operation and tube picking operation. The specific steps for "executing the sample box exchange and tube picking mode" include:

[0112] S32431: Perform a sample box exchange operation. Exchange the storage locations of the sample boxes to be transferred that contain complete boxes of samples with the empty sample boxes.

[0113] S32432: Perform the sample picking operation. Pick the sample tubes from the sample box to be transferred that do not contain a full box of samples and store them in an empty sample box and / or store the sample tubes from the sample box to be transferred that do not contain a full box of samples in a half-stored sample box in the target storage repository.

[0114] This setup, which involves performing the sample box exchange operation first and then the tube picking operation, can save the time required for sample transfer and ensure sample safety to the greatest extent possible.

[0115] Preferably, please refer to Figure 7 The specific steps for "performing a sample box exchange operation" include:

[0116] S324311: Obtain the number of second sample boxes to be transferred in the sample boxes to be transferred, denoted as the number of second sample boxes to be transferred, where the second sample box to be transferred is a sample box to be transferred that stores a whole box of sample tubes to be transferred.

[0117] S324312: Based on the number of second sample boxes to be transferred, the number of multiple spare sample boxes, and the distance relationship between each target repository and the repository to be transferred, determine the number of second target boxes that each target repository needs to receive.

[0118] Specifically, the system prioritizes selecting the target repository closest to the repository to be transferred, and only selects the next closest target repository after the nearest target repository can no longer receive the data. This setting can save the time required for the transfer of sample boxes during the transfer process, thereby improving the transfer efficiency.

[0119] S324313: Based on the number of second target boxes that each target repository needs to receive, determine the second target spare sample boxes in each target repository that need to be swapped with the second sample boxes to be transferred.

[0120] S324314: Match and bind each second sample box to be transferred with a second target empty sample box.

[0121] Specifically, the binding can be based on the location information of each second sample box to be transferred and each second target empty sample box, or it can be based on the box code information of the second sample box to be transferred and the second target empty sample box.

[0122] S324315: Swap the storage locations of the bound second sample box to be transferred and the second target empty sample box.

[0123] In addition, during the exchange process, both sample boxes are scanned to determine if the exchange is successful, ensuring the accuracy of the exchange and timely updating of management information within the control system.

[0124] This setup binds each second sample box to be transferred to a second target empty sample box and allows for their interchange, enabling orderly transfer and facilitating control and updating of stored information.

[0125] Preferably, please refer to Figure 8The specific steps for "performing the pipe-picking operation" include:

[0126] S324321: Obtain the total number of samples to be transferred in all the sample boxes to be transferred, denoted as the total number of samples to be transferred. The samples to be transferred are the samples to be transferred stored in the third sample box to be transferred. The third sample box to be transferred is a sample box to be transferred that contains sample tubes to be transferred but is not full.

[0127] S324322: Obtain the number of empty sample boxes in each target repository again to get the number of multiple empty sample boxes for picking tubes.

[0128] S324323: Determine the number of tubes to be received based on the number of available sample boxes for multiple tube picking and the storage capacity of each sample box.

[0129] S324324: Selectively perform either the first paired tube picking operation or the second paired tube picking operation based on the tube picking quantity and the total number of tubes picked.

[0130] The first pairing tube picking operation specifically includes picking all the samples to be picked into the empty sample box; and returning the empty sample box to its original storage location when it is full.

[0131] The second pairing and picking operation specifically includes dividing all samples to be picked into a first picking section and a second picking section. First, the samples to be picked from the first picking section are picked into empty sample boxes. Specifically, when an empty sample box is full, it is returned to its original storage location. Then, the samples to be picked from the second picking section are picked into half-storage sample boxes in the target storage box. Specifically, when a half-storage sample box is full, it is returned to its original storage location. This process continues until all half-storage sample boxes are full or all samples to be picked have been transferred.

[0132] With this setup, during the tube picking operation, the first or second pairing tube picking operation can be selectively performed based on the number of tubes received and the total number of tubes picked. This setup determines the appropriate tube picking mode for transfer based on the total number of tubes picked and the number of tubes received, thereby saving transfer time and ensuring sample safety.

[0133] Preferably, step S324324, "selectively performing a first pairing tube picking operation or a second pairing tube picking operation based on the tube receiving quantity and the total number of tubes picked," specifically includes:

[0134] S3243241: Compare the number of tubes received with the total number of tubes picked.

[0135] S3243242: If the number of tubes received is not less than the total number of tubes to be picked, then the first pairing tube picking operation is performed.

[0136] S3243243: If the number of tubes received is less than the total number of tubes to be picked, then perform the second pairing tube picking operation.

[0137] This setup makes the judgment process simple, easy to execute, and fast.

[0138] Furthermore, during the second pairing and picking operation, the control method of the present invention further includes:

[0139] S4: Obtain the total number of samples to be transferred in the second tube-picking section, denoted as the second tube-picking total.

[0140] S5: Obtain the total number of semi-storage sample boxes received in all target repositories, denoted as the second pick-and-pick received number.

[0141] S6: Selectively trigger an emergency alarm based on the total amount of the second pick-up tube and the amount received by the second pick-up tube.

[0142] During the second pairing tube picking operation, an emergency alarm is selectively triggered based on the total number of tubes picked and the number of tubes received. An alarm is triggered when the total number of tubes picked exceeds the number of tubes received, so as to remind the user to transfer the excess samples in time and minimize losses.

[0143] Preferably, step S6, "selectively triggering an emergency alarm based on the total amount of the second pick-up tube and the amount of the second pick-up tube received," specifically includes:

[0144] S61: Compare the total amount of the second pick-up tube and the amount received by the second pick-up tube.

[0145] S62: If the total amount of the second pick tube is not greater than the amount received by the second pick tube, no emergency alarm will be triggered.

[0146] S63: If the total amount of the second pick tube exceeds the amount received by the second pick tube, an emergency alarm will be triggered.

[0147] Preferably, the steps of "performing auxiliary cooling operations" specifically include:

[0148] S331: Calculate the difference between the actual temperature and the first preset temperature.

[0149] S332: Determine the amount of liquid nitrogen introduced based on the difference.

[0150] S333: Liquid nitrogen is introduced into the storage unit experiencing temperature imbalance faults according to the injection rate to lower the temperature inside the storage unit.

[0151] By setting it up in this way, the amount of liquid nitrogen introduced is determined based on the difference, and liquid nitrogen is introduced into the storage tank with temperature imbalance according to this amount. This can ensure a good cooling effect while saving liquid nitrogen consumption and reducing costs.

[0152] The technical solution of the present invention has been described above with reference to the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the scope of protection of the present invention is obviously not limited to these specific embodiments. Without departing from the principles of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after such changes or substitutions will all fall within the scope of protection of the present invention.

Claims

1. A control method of a biological sample storage system, characterized by, The biological sample storage system comprises a plurality of storage banks, the plurality of storage banks are spaced apart along a linear structure, two adjacent storage banks are connected by a connecting channel and can transfer sample boxes to each other; The control method comprises: Real-time acquisition of the temperature of each storage bank, obtaining a plurality of actual temperatures; According to the plurality of actual temperatures, it is judged whether there is a temperature imbalance fault in the storage bank; In the case where the storage bank has the temperature imbalance fault, according to the actual temperature of the storage bank that has the temperature imbalance fault, selectively execute auxiliary cooling operation or sample transfer operation; Wherein, the auxiliary cooling operation is specifically through the way of providing liquid nitrogen into the storage bank to assist in cooling; the sample transfer operation is specifically to transfer the sample stored in the storage bank to be transferred to the target storage bank, wherein the storage bank to be transferred is the storage bank that has the temperature imbalance fault, and the target storage bank is the storage bank that does not have the temperature imbalance fault; The step of "executing sample transfer operation" specifically comprises: Obtaining the total number of the sample boxes to be transferred occupied by the samples to be transferred, denoted as the number of sample boxes to be transferred; Obtaining the number of empty sample boxes in each target storage bank, obtaining a plurality of empty sample box numbers; Adding up all the empty sample box numbers to obtain the total number of empty sample boxes; According to the number of sample boxes to be transferred and the total number of empty sample boxes, selectively execute sample box exchange mode or sample box exchange composite pick tube mode; Wherein, the sample box exchange mode specifically includes exchanging the storage location of the sample box to be transferred and the empty sample box; the sample box exchange composite pick tube mode specifically includes exchanging the storage location of the sample box to be transferred and the empty sample box which stores the whole box of samples, and picking and storing the sample to be transferred in the sample box to be transferred which does not store the whole box of samples into the empty sample box and / or picking and storing the sample to be transferred in the sample box to be transferred which does not store the whole box of samples into the half storage sample box in the target storage.

2. The control method of the biological sample storage system according to claim 1, wherein The step of "judging whether there is a temperature imbalance fault in the storage bank according to the plurality of actual temperatures" specifically comprises: Comparing each actual temperature with a first preset temperature; If the actual temperature is higher than the first preset temperature, it is determined that the storage bank corresponding to the actual temperature has the temperature imbalance fault; If the actual temperature is not higher than the first preset temperature, it is determined that the storage bank corresponding to the actual temperature does not have the temperature imbalance fault.

3. The control method of the biological sample storage system according to claim 2, wherein The step of "selectively executing auxiliary cooling operation or sample transfer operation according to the actual temperature of the storage bank that has the temperature imbalance fault" specifically comprises: Comparing the actual temperature of the storage bank that has the temperature imbalance fault with a second preset temperature; If the actual temperature is not higher than the second preset temperature, execute the auxiliary cooling operation; If the actual temperature is higher than the second preset temperature, execute the sample transfer operation; The second preset temperature is higher than the first preset temperature.

4. The control method of the biological sample storage system according to claim 1, wherein The step of "executing the sample box exchange mode" specifically comprises: According to the number of the sample boxes to be transferred, the number of the empty sample boxes, and the distance relationship of each target storage relative to the sample box to be transferred, the first target number of sample boxes to be transferred that each target storage needs to receive is determined; According to the first target number of sample boxes to be transferred that each target storage needs to receive, the first target empty sample box that each target storage needs to exchange positions with the sample box to be transferred is determined; Each sample box to be transferred is matched and bound with a first target empty sample box; The bound sample box to be transferred and the first target empty sample box are exchanged in storage position.

5. The control method of the biological sample storage system according to claim 1, wherein The sample box exchange composite pipetting mode comprises a sample box exchange operation and a pipetting operation, and the step of "executing the sample box exchange composite pipetting mode" specifically comprises: The sample box exchange operation is executed; The pipetting operation is executed.

6. The control method of a biological sample storage system according to claim 5, wherein The step of "executing the sample box exchange operation" specifically comprises: The number of the second sample boxes to be transferred in the sample boxes to be transferred is obtained, denoted as the second number of sample boxes to be transferred, wherein the second sample box to be transferred is the sample box to be transferred that stores a whole set of sample tubes to be transferred; According to the second number of sample boxes to be transferred, the number of the empty sample boxes, and the distance relationship of each target storage relative to the sample box to be transferred, the second target number of sample boxes to be transferred that each target storage needs to receive is determined; According to the second target number of sample boxes to be transferred that each target storage needs to receive, the second target empty sample box that each target storage needs to exchange positions with the second sample box to be transferred is determined; Each second sample box to be transferred is matched and bound with a second target empty sample box; The bound second sample box to be transferred and the second target empty sample box are exchanged in storage position.

7. The control method of the biological sample storage system according to claim 5, wherein The step of "executing the pipetting operation" specifically comprises: The total number of samples to be pipetted in the sample boxes to be transferred is obtained, denoted as the total number of samples to be pipetted, wherein the sample to be pipetted is the sample to be transferred stored in a third sample box to be transferred, and the third sample box to be transferred is the sample box to be transferred that stores sample tubes to be transferred but is not full of a whole set; The number of empty sample boxes in each target storage is obtained again, obtaining a plurality of numbers of empty sample boxes for pipetting; According to the numbers of empty sample boxes for pipetting and the storage amount of each sample box, the pipetting receiving amount is determined; According to the pipetting receiving amount and the total number of samples to be pipetted, the first paired pipetting operation or the second paired pipetting operation is selectively executed; The first paired pipetting operation specifically comprises pipetting all the samples to be pipetted into the empty sample boxes; The second paired pipetting operation specifically includes: dividing all the to-be-pipetted transfer samples into a first pipetting part and a second pipetting part, and first pipetting the to-be-pipetted transfer samples in the first pipetting part into the empty sample box; and then pipetting the to-be-pipetted transfer samples in the second pipetting part into the semi-storage sample box in the target storage library.

8. The control method of the biological sample storage system according to claim 7, wherein In the process of performing the second paired pipetting operation, the control method further includes: obtaining the total number of the to-be-pipetted transfer samples in the second pipetting part, denoted as a second pipetting total amount; obtaining the total receiving amount of the semi-storage sample boxes in all the target storage libraries, denoted as a second pipetting receiving amount; selectively performing an emergency alarm according to the second pipetting total amount and the second pipetting receiving amount.

9. A biological sample storage system comprising a controller, characterized in that, The controller is configured to perform the control method of the biological sample storage system according to any one of claims 1 to 8.

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