On-orbit microbial culture fluid circuit and control method and control device thereof

Abstract

The present invention relates to the field of space science experiment technology, and provides an on-orbit microbial culture liquid circuit, a control method thereof, and a control device therefor. The on-orbit microbial culture liquid circuit comprises: a culture tank having a liquid inlet and an air outlet; a storage device, formed with a liquid storage cavity and an air storage cavity, the liquid storage cavity and the air storage cavity being isolated by a flip membrane, a liquid outlet being formed on the liquid storage cavity, and an air inlet being formed on the air storage cavity; wherein the liquid outlet of the liquid storage cavity is connected to the liquid inlet of the culture tank through a liquid pipeline, the air outlet of the culture tank is connected to the air inlet of the air storage cavity through a gas pipeline, and a pump body is provided on at least one of the liquid pipeline and the gas pipeline. The on-orbit microbial culture liquid circuit, the control method thereof, and the control device thereof balance the internal pressure by the deformation of the flip membrane, ensure the stability of the culture environment, and provide stable growth conditions for microorganisms. It can also be applied to special environments with microgravity or zero gravity, such as space stations, outer space, and on-orbit environments.

Description

Technical Field

[0001] The present invention relates to the technical field of space science experiments, and in particular to an on-orbit microbial culture fluid circuit and a control method and a control device thereof. Background Art

[0002] The space station provides an irreplaceable and unique environment for conducting microbial experiments. The stable microgravity, vacuum, and radiation conditions provided by space can bring new and significant breakthroughs to microbial science experiments.

[0003] Due to the particularity of conducting microbial experiments on orbit, microbial cultivation on orbit faces a series of problems. First of all, all test devices must be closed structures, and there are strict requirements for the sealing of the devices. This requires a complete liquid control system and gas-liquid circulation structure inside the device. Currently, the commonly used gas-liquid circulation system often requires additional waste gas bags and micro pumps to actively discharge waste gas and waste liquid. This method increases the size of the test device and requires additional operations. On-orbit testing has high requirements for the weight, size, power consumption, and ease of operation for astronauts. Therefore, it is necessary to use smaller and more automated test devices to improve test efficiency and space utilization. Summary of the Invention

[0004] The present invention provides an on-orbit microbial culture fluid circuit and its control method and control device, which are used to address the defects existing in the existing technology and achieve the following effects: balancing the internal pressure by flipping the deformation of the membrane, ensuring the stability of the culture environment, and providing stable growth conditions for microorganisms. It can also be applied to special environments such as microgravity or zero gravity, such as space stations, outer space, and on-orbit environments.

[0005] An on-orbit microbial culture fluid circuit according to an embodiment of the first aspect of the present invention comprises:

[0006] a culture tank having a liquid inlet and a gas outlet;

[0007] A storage device is formed with a liquid storage cavity and an air storage cavity, wherein the liquid storage cavity and the air storage cavity are isolated from each other by a flip membrane, a liquid outlet is formed on the liquid storage cavity, and an air inlet is formed on the air storage cavity;

[0008] The liquid outlet of the liquid storage cavity is connected to the liquid inlet of the culture tank through a liquid pipe, and the gas outlet of the culture tank is connected to the gas inlet of the gas storage cavity through a gas pipe, wherein at least one of the liquid pipe and the gas pipe is provided with a pump body.

[0009] According to one embodiment of the present invention, a water pump is provided on the liquid pipeline.

[0010] According to one embodiment of the present invention, a one-way valve is provided between the water pump and the liquid inlet of the culture tank, and the valve direction of the one-way valve is oriented along the direction of the water pump toward the liquid inlet.

[0011] According to one embodiment of the present invention, a temperature regulating module and a temperature detecting module are provided outside the culture tank.

[0012] According to one embodiment of the present invention, a flow meter is provided on the liquid pipeline, and / or an air pressure detection module is provided outside the culture tank.

[0013] According to the first embodiment of the present invention, a method for controlling an on-orbit microbial culture fluid circuit based on the first embodiment of the present invention includes:

[0014] Upon receiving a signal for starting microbial culture, the pump body is controlled to start so that the on-orbit microbial culture fluid path enters a culture mode;

[0015] In the culture mode, the culture parameter information in the culture pool is monitored, and the working parameters of the pump body are adjusted in real time according to the culture parameter information.

[0016] According to one embodiment of the present invention, in the step of monitoring the culture parameter information in the culture pool in the microorganism culture mode: the culture parameter information in the culture pool includes culture environment information and / or microorganism information in the culture pool.

[0017] According to one embodiment of the present invention, the culture environment information includes the size of the air pressure in the pool, and the step of adjusting the working parameters of the pump body in real time according to the culture parameter information specifically includes:

[0018] When the air pressure in the pool exceeds the maximum set air pressure, the power of the pump body is controlled to decrease until the air pressure in the pool is less than the maximum set air pressure;

[0019] When the air pressure in the pool is lower than the minimum set air pressure, the power of the pump body is controlled to increase until the air pressure in the pool is greater than the minimum set air pressure.

[0020] According to one embodiment of the present invention, the microbial information includes microbial growth rate, and the step of adjusting the working parameters of the pump body in real time according to the culture parameter information specifically includes:

[0021] When the microbial growth rate is lower than the target growth rate, the power of the pump is controlled to increase until the microbial growth rate reaches the target growth rate;

[0022] When the growth rate of the microorganisms reaches or exceeds the target growth rate, the power of the pump body is controlled to remain unchanged.

[0023] According to one embodiment of the present invention, the culture environment information includes the temperature in the pool, and the microbial information includes the microbial growth rate. After receiving the signal to start microbial culture, and controlling the pump to start so that the on-track microbial culture fluid circuit enters the culture mode, the following steps are further included:

[0024] In the culture mode, the temperature in the culture tank is adjusted by the temperature adjustment module according to the range of the growth rate of the microorganisms.

[0025] According to one embodiment of the present invention, after the step of adjusting the working parameters of the pump in real time according to the culture parameter information, the method further includes:

[0026] When the culturing time of the microorganisms in the culturing tank reaches the target culturing period, controlling the pump body to shut down;

[0027] The target cultivation period is obtained as follows:

[0028] Obtaining the types of microorganisms in the culture tank and the target culture quantity of the microorganisms;

[0029] Calculating the total amount of culture solution required for the microorganisms in the culture tank based on the microorganism species and the target culture quantity;

[0030] The target culture period required for the microorganisms in the culture tank is calculated based on the total amount of the culture solution and the power of the pump.

[0031] According to a third aspect of the present invention, a control device for an on-orbit microbial culture fluid circuit based on the first aspect of the present invention includes:

[0032] a first control module, configured to receive a microorganism culture start signal and control the pump body to start so that the on-orbit microorganism culture liquid circuit enters a culture mode;

[0033] The second control module is used to monitor the culture parameter information in the culture pool in the culture mode, and adjust the working parameters of the pump body in real time according to the culture parameter information.

[0034] According to the on-orbit microbial culture fluid circuit of the embodiment of the present invention, the system can automatically adjust the volume of the liquid and gas storage chambers, balancing internal pressure through the deformation of the flip membrane, ensuring a stable culture environment. It can also maintain a suitable environment within the culture tank, providing stable growth conditions for microorganisms and helping to improve the success rate of experiments. Furthermore, this fluid circuit system reduces the risk of microbial contamination and cross-infection through physical isolation and pressure balancing mechanisms.

[0035] Furthermore, the on-orbit microbial culture fluid circuit of the present invention can also be applied to special environments with microgravity or zero gravity, such as space stations, outer space, and on-orbit environments. This fluid circuit system achieves automatic pressure balance through the design of a flip membrane and a water pump. This ensures that the liquid and gas inside the culture tank remain properly separated and circulated even in the absence of gravity, thereby maintaining the environment required for microbial growth. Furthermore, because this fluid circuit system utilizes a closed circulation system, all liquids and gases circulate within the system, reducing dependence on the external environment. This prevents leakage of liquids and gases in a microgravity environment, ensuring the safety and reliability of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0037] Figure 1 This is a schematic diagram of the structure of the on-orbit microbial culture fluid circuit provided by the present invention;

[0038] Figure 2 This is a schematic diagram of the steps of the method for controlling the on-orbit microbial culture fluid circuit provided by the present invention;

[0039] Figure 3 This is a schematic structural diagram of the control device for the on-orbit microbial culture fluid circuit provided by the present invention;

[0040] Figure 4 It is a structural schematic diagram of the electronic device provided by the present invention.

[0041] Reference numerals:

[0042] 1. Culture tank; 2. Storage device; 21. Liquid storage chamber; 22. Gas storage chamber; 23. Flip membrane; 41. Liquid pipeline; 42. Gas pipeline; 5. Water pump; 6. One-way valve; 7. Flow meter; 8. Temperature detection module; 9. Air pressure detection module; 110. First control module; 120. Second control module. DETAILED DESCRIPTION

[0043] To make the objectives, technical solutions, and advantages of the present invention more clear, the technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0044] In the description of the embodiments of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore should not be understood as limiting the embodiments of the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and should not be understood as indicating or implying relative importance.

[0045] The following describes an on-orbit microbial culture fluid circuit, a control method and a control device for an on-orbit microbial culture fluid circuit provided by the present invention with reference to the accompanying drawings. It should be noted that the control method introduced in the embodiment of the second aspect of the present invention and the control device introduced in the embodiment of the third aspect of the present invention are both implemented based on the on-orbit microbial culture fluid circuit introduced in the embodiment of the first aspect of the present invention.

[0046] like Figure 1 As shown, the on-orbit microbial culture fluid circuit according to the first embodiment of the present invention includes a culture tank 1, a storage device 2, a gas pipeline 42 and a liquid pipeline 41.

[0047] The culture tank 1 has a liquid inlet and an air outlet, and is used to provide a closed culture environment for microbial cultivation. The storage device 2 is formed with a liquid storage chamber 21 and an air storage chamber 22, which are separated by a reversible membrane 23. The liquid storage chamber 21 has a liquid outlet, and the air storage chamber 22 has an air inlet. The liquid storage chamber 21 stores the culture fluid required for microbial cultivation.

[0048] The liquid outlet of the liquid storage chamber 21 is connected to the liquid inlet of the culture tank 1 through the liquid pipe 41, and the gas outlet of the culture tank 1 is connected to the gas inlet of the gas storage chamber 22 through the gas pipe 42, wherein at least one of the liquid pipe 41 and the gas pipe 42 is provided with a pump body.

[0049] It can be understood that for the on-orbit microbial culture fluid circuit, the main function of the culture tank 1 is to provide a closed culture environment for microorganisms to ensure their growth under sterile conditions. The storage device 2 consists of two main parts: a liquid storage chamber 21 and an air storage chamber 22. These two chambers are isolated from each other by a flip membrane 23 to maintain the pressure balance inside each chamber. The liquid storage chamber 21 stores the culture fluid for microbial culture, while the air storage chamber 22 is used to store gas. The flip membrane 23 is a key component of the storage device 2. It separates the liquid storage chamber 21 and the air storage chamber 22, allowing liquid and gas to circulate independently in their respective chambers while maintaining the internal pressure balance of the entire system. The flip membrane 23 is provided with a protrusion protruding toward the air storage chamber. When the gas in the air storage chamber 22 presses the protrusion, it can be deformed and flipped, completely recessed to the side of the liquid storage chamber 21.

[0050] During microbial cultivation, the liquid conduit 41 allows the culture fluid to flow from the storage device 2 into the culture tank 1, providing the microorganisms with necessary nutrients. The gas conduit 42 allows gas to be discharged from the culture tank 1 into the gas storage chamber 22, thereby maintaining the pressure within the culture tank 1. The pump body is used to control the flow of liquid or gas, ensuring that the culture fluid and gas can enter and leave the culture tank 1 at a predetermined rate and volume.

[0051] According to the on-orbit microbial culture liquid circuit of an embodiment of the present invention, its specific working process is as follows: at the beginning of the experiment, the pump body is started, and the culture liquid in the liquid storage chamber 21 is transported to the liquid inlet of the culture tank 1 through the liquid pipe 41 to start microbial culture. Microorganisms grow in the culture tank 1 and produce waste gas (such as carbon dioxide) during metabolism. These waste gases accumulate in the culture tank 1, causing the internal pressure of the culture tank 1 to rise. As the internal pressure of the culture tank 1 increases, the gas pipeline will transport the excess gas inside the culture tank 1 to the gas storage chamber 22. At this time, the volume of the gas storage chamber 22 will increase accordingly to accommodate more gas. At the same time, the volume of the liquid storage chamber 21 decreases, and the flip membrane 23 deforms to adapt to the change in the volume of the two cavities, thereby maintaining the pressure balance of the entire system. In some cases, if the pressure in the gas storage chamber 22 is too high, the excess gas can be discharged through a specific discharge mechanism to prevent excessive pressure from causing damage to the system. After the culture is completed, the pump body is closed and the liquid delivery stops. The flip membrane 23 returns to its initial state according to the volume changes of the liquid storage chamber 21 and the gas storage chamber 22, and the system is ready for the next experiment or maintenance.

[0052] According to the on-orbit microbial culture fluid circuit of the embodiment of the present invention, the system can automatically adjust the volume of the liquid storage chamber 21 and the air storage chamber 22, balancing the internal pressure by deforming the inverting membrane 23, ensuring a stable culture environment. It can also maintain a suitable environment within the culture tank 1, providing stable growth conditions for microorganisms and helping to improve the success rate of experiments. Furthermore, this fluid circuit system reduces the risk of microbial contamination and cross-infection through physical isolation and pressure balancing mechanisms.

[0053] Furthermore, the on-orbit microbial culture fluid circuit of the present invention can also be applied to special environments with microgravity or zero gravity, such as space stations, outer space, and on-orbit environments. It is understood that in microgravity or zero gravity environments, liquids and gases behave differently than on Earth and do not naturally separate. However, this fluid circuit system achieves automatic pressure balancing through the design of the inverted membrane 23 and the water pump 5. This ensures that the liquid and gas within the culture tank 1 remain properly separated and circulated, even in the absence of gravity, thereby maintaining the environment required for microbial growth.

[0054] On the one hand, because this fluid circuit system utilizes a closed circulation system, all liquids and gases circulate within the system, reducing reliance on the external environment. This prevents leakage of liquids and gases in microgravity, ensuring the safety and reliability of experiments. On the other hand, because manual operations in microgravity are more difficult than on Earth, the design of this fluid circuit system reduces reliance on astronauts' operations. Therefore, the automated control system of the present invention simplifies the experimental process and reduces operational complexity.

[0055] Furthermore, applying the present invention's on-orbit microbial culture fluid circuit to a microgravity environment offers the following additional advantages: First, given the limited space within a space station, the system's compact design helps maximize space utilization while reducing the spacecraft's payload. Second, the system design takes into account the particularities of the on-orbit environment, such as temperature, radiation, and vacuum conditions, ensuring stable operation even under these extreme conditions. Third, energy consumption is a consideration for on-orbit equipment, and the system's low-power design helps extend the spacecraft's operating time and reduce its reliance on energy supplies.

[0056] like Figure 1 As shown, according to some embodiments of the present invention, a water pump 5 is provided on the liquid pipeline 41. In the embodiment, the water pump 5 on the liquid pipeline 41 can effectively transport liquid in a microgravity environment.

[0057] Furthermore, the water pump 5 is a peristaltic pump. A peristaltic pump periodically squeezes a pipe, generating pressure waves that propel the liquid through the pipe. This type of pump is suitable for transporting various types of liquids, including culture fluids, and can function normally even in a microgravity environment.

[0058] In this way, by using a peristaltic pump as a liquid delivery power source, precise flow control can be provided. Moreover, since microbial growth requires a precise supply of nutrients, by adjusting the operating parameters of the pump, it is possible to ensure that the culture fluid enters the culture tank 1 at a constant rate, thereby ensuring precise control of the culture fluid supply.

[0059] In addition, a notable feature of peristaltic pumps is that there is no direct contact between the pump body and the liquid, which reduces the risk of contamination, especially in sterile microbial culture environments.

[0060] Furthermore, peristaltic pumps have the following advantages: low power consumption, simple structure, relatively easy maintenance and replacement of pump tubes, and the ability to operate under various temperature and pressure conditions, thus ensuring that the pump body can operate stably in extreme environments such as space stations.

[0061] like Figure 1 As shown, according to some embodiments of the present invention, a one-way valve 6 is provided between the water pump 5 and the liquid inlet of the culture tank 1 , and the valve direction of the one-way valve 6 is oriented along the direction of the water pump 5 toward the liquid inlet.

[0062] In this embodiment, the design of the one-way valve 6 ensures that liquid can only flow from the water pump 5 to the liquid inlet of the culture tank 1, preventing the liquid from flowing back when the pump stops working, thereby helping to maintain a stable pressure inside the culture tank 1. In addition, the addition of the one-way valve 6 can improve the reliability of the entire liquid circuit control system and reduce the risk of liquid leakage due to improper pump operation or system failure. It will be understood that when the pump stops working, the one-way valve 6 needs to be closed to prevent the pressure inside the culture tank 1 from changing.

[0063] According to some embodiments of the present invention, the culture tank 1 cavity is a sealed chamber having a liquid inlet and an air outlet. Preferably, the culture tank 1 cavity is made of an aluminum alloy or other metal material. Such metal materials have high strength and good corrosion resistance, capable of maintaining structural integrity and stability in the harsh space environment. Furthermore, the thermal conductivity of the metal material helps maintain temperature uniformity within the culture tank 1.

[0064] According to some embodiments of the present invention, both the gas pipeline 42 and the liquid pipeline 41 are made of latex tubes or soft silicone tubes. It is understandable that latex tubes or soft silicone tubes have good flexibility, can adapt to the layout and movement of pipelines in a microgravity environment, and reduce the risk of rupture due to hardening or embrittlement of the pipeline. In addition, these soft materials generally have good chemical stability and will not react with the culture medium or gas, thereby ensuring the accuracy of the experiment. In addition, latex tubes and silicone tubes generally have good biocompatibility and will not have a negative impact on the growth of microorganisms. Furthermore, pipelines made of soft materials are more convenient to install and maintain, especially in a small and operationally restricted environment such as a space station.

[0065] like Figure 1 As shown, according to some embodiments of the present invention, a temperature regulating module and a temperature detecting module 8 are provided outside the culture pool 1 .

[0066] In this embodiment, the temperature detection module 8 is used to monitor the temperature within the culture tank 1 in real time, ensuring that the temperature remains within a set range. Furthermore, the temperature data detected by the temperature detection module 8 serves as a feedback signal to adjust the output of the temperature regulation module, achieving closed-loop control and ensuring temperature stability. Furthermore, the temperature regulation module can precisely control the temperature within the culture tank 1 and, by maintaining a suitable temperature, ensure that microorganisms are cultured under optimal growth conditions, thereby improving experimental success rates and data accuracy.

[0067] In this way, the temperature detection module 8 and the temperature adjustment module are combined with the automatic control system to achieve unattended continuous temperature monitoring and adjustment, reducing the operating burden.

[0068] like Figure 1 As shown, according to some embodiments of the present invention, a flow meter 7 is provided on the liquid pipeline 41 , and an air pressure detection module 9 is provided outside the culture tank 1 .

[0069] Flow meter 7 accurately measures the flow rate of liquid through the infusion pipeline, providing real-time flow data to help researchers monitor and adjust the culture process to optimize microbial growth conditions. This flow data also serves as system feedback, automatically adjusting pump operating parameters and achieving precise control of the culture fluid supply.

[0070] The air pressure detection module 9 is used to monitor the air pressure changes inside the culture tank 1. By monitoring the air pressure, possible pressure problems, such as excessively high or low pressure, can be discovered and resolved in a timely manner, helping to ensure the safe operation of the entire culture system and prevent potential risks caused by abnormal pressure.

[0071] The control method and control device for the on-orbit microbial culture fluid circuit proposed by the present invention will be described below with reference to the accompanying drawings. Before describing the embodiments of the present invention in detail, the entire application scenario will be described first. The control method, control device, electronic device and computer-readable storage medium for the on-orbit microbial culture fluid circuit of the embodiments of the present invention can be applied locally to the on-orbit microbial culture fluid circuit, can be applied to cloud platforms in the Internet field, or other types of cloud platforms in the Internet field, or can also be applied to third-party devices. Among them, third-party devices may include various types such as mobile phones, tablet computers, notebooks, vehicle-mounted computers and other smart terminals.

[0072] The following description only uses the control method applicable to the on-orbit microbial culture fluid circuit as an example. It should be understood that the control method of the embodiment of the present invention can also be applied to cloud platforms and third-party devices.

[0073] like Figure 2 As shown, the control method of the on-orbit microorganism culture fluid circuit according to the second embodiment of the present invention includes:

[0074] Step S1, receiving a signal for starting microbial culture, controlling the pump to start so that the on-track microbial culture fluid path enters a culture mode;

[0075] Step S2: In the culture mode, the culture parameter information in the culture pool 1 is monitored, and the working parameters of the pump body are adjusted in real time according to the culture parameter information.

[0076] According to an embodiment of the present invention, the control method for controlling an on-orbit microbial culture fluid circuit operates as follows: upon receiving a signal to initiate microbial culture, the control system activates and the pump is started, which then transports the culture fluid from storage device 2 to culture tank 1. The pump can be activated manually or automatically, for example, by a sensor detecting the initial level of the culture fluid in culture tank 1 or by a preset schedule.

[0077] In the culture mode, the system will continuously monitor the culture parameters in the culture pool 1, such as temperature, pH value, oxygen concentration, etc. At this time, the temperature detection module 8 and the air pressure detection module 9 will collect data in real time and transmit the data to the control system.

[0078] Based on the collected culture parameter information, the control system uses an algorithm to analyze and determine whether adjustments to the pump's operating parameters are necessary. For example, if the temperature is below a set point, the system might increase the pump flow rate to raise the culture fluid temperature. If the oxygen concentration is insufficient, the system might adjust the flow rate in gas pipeline 42 to increase the oxygen supply. It should be noted that these adjustments can be continuous or periodic, depending on the microbial growth stage and experimental design.

[0079] Furthermore, in some cases, the system may also need to monitor and adjust the nutritional composition of the culture medium, such as by adding specific nutrients or adjusting the concentration of the culture medium. The system can also integrate an automated sample collection mechanism to regularly sample from the culture tank 1 for analysis, thereby providing more comprehensive monitoring of the culture process.

[0080] According to the on-orbit microbial culture fluid control method of the present invention, through real-time monitoring and adjustment, the system can ensure that culture conditions are always optimal, thereby improving the efficiency of microbial growth and the quality of experimental results. Furthermore, automated control reduces the need for manual intervention, reduces the risk of operational errors, and improves experimental repeatability.

[0081] In addition, the system can adjust culture conditions according to different microbial species and growth stages, showing high flexibility and adaptability. Automated monitoring and adjustments within the system help maintain long-term culture stability and facilitate the long-term conduct of on-orbit experiments.

[0082] According to some embodiments of the present invention, in the step of monitoring the culture parameter information in the culture tank 1 in the microorganism culture mode: the culture parameter information in the culture tank 1 includes culture environment information and / or microorganism information in the culture tank 1.

[0083] Culture environment information can include parameters such as temperature, pH, oxygen concentration, carbon dioxide concentration, and humidity. It is understood that these environmental factors have a direct impact on microbial growth and metabolic activity. Microbial information can include microbial growth rate, biomass, and metabolite production. It is understood that this information helps assess the health of microorganisms and the effectiveness of culture.

[0084] In this way, by monitoring the culture environment and microbial information, the system can provide a comprehensive assessment of the culture status and ensure the accuracy of the experiment.

[0085] In some specific embodiments of the present invention, the culture environment information includes the size of the air pressure in the pool, and the step of adjusting the working parameters of the pump body in real time according to the culture parameter information specifically includes:

[0086] When the air pressure in the pool exceeds the maximum set pressure, the power of the control pump body is reduced until the air pressure in the pool is lower than the maximum set pressure;

[0087] When the air pressure in the pool is lower than the minimum set pressure, the power of the pump body is controlled to increase until the air pressure in the pool is greater than the minimum set pressure.

[0088] In this embodiment, the air pressure detection module 9 monitors the air pressure in the culture tank 1 in real time.

[0089] At some point during the experiment, the gas produced by microbial metabolism accumulates, causing the air pressure in culture tank 1 to reach the set maximum. Upon receiving this information, the control system automatically reduces the pump power, reducing the amount of liquid delivered, thereby reducing the amount of gas entering culture tank 1. This, in turn, lowers the air pressure in culture tank 1, preventing it from exceeding a safe range. As the pump power decreases, the air pressure in culture tank 1 begins to drop. The control system continues to monitor the air pressure until it drops below the maximum set value. At this point, the pump power remains at a low level to maintain stable air pressure.

[0090] If, at other times, the air pressure detection module 9 indicates that the air pressure in culture tank 1 is below the minimum set value, this indicates that the amount of gas in culture tank 1 may be insufficient, potentially affecting microbial growth. In this case, the control system will immediately respond by increasing the pump power, increasing the liquid delivery rate, and thereby increasing the amount of gas entering culture tank 1 and raising the air pressure. As the air pressure gradually rises, the control system will continue to monitor and adjust the pump power until the air pressure returns to above the minimum set value.

[0091] In other embodiments of the present invention, the microbial information includes the microbial growth rate, and the step of adjusting the operating parameters of the pump in real time according to the culture parameter information specifically includes:

[0092] When the microbial growth rate is lower than the target growth rate, the power of the pump body is controlled to increase until the microbial growth rate reaches the target growth rate;

[0093] When the growth rate of the microorganisms reaches or exceeds the target growth rate, the power of the pump body is controlled to remain unchanged.

[0094] In this embodiment, the monitoring of the microorganism growth rate and the adjustment of the pump operating parameters are to ensure that the microorganisms grow at a predetermined rate in the culture tank 1 .

[0095] For example, the specific working process is as follows: using a microscope or other imaging technology to regularly observe and record the morphology and quantity of microorganisms in the culture tank 1 to calculate the growth rate, wherein the growth rate can be determined by comparing the number or biomass of microorganisms at different time points.

[0096] When the monitored microbial growth rate falls below the preset target, the control system receives this information. To promote growth, the control system adjusts the pump power, increasing the volume of culture fluid delivered, thereby providing more nutrients and possibly oxygen. This adjustment continues until the microbial growth rate reaches or approaches the target.

[0097] When the microbial growth rate reaches or exceeds the target growth rate, the control system will keep the pump power constant to maintain the current growth conditions. This ensures that the microorganisms continue to grow at the optimal growth rate while avoiding the waste of resources or deterioration of the growth environment that may result from excessive growth.

[0098] According to some embodiments of the present invention, the culture environment information includes the temperature in the pool, and the microbial information includes the microbial growth rate. After receiving the microbial culture start signal and controlling the pump to start so that the on-track microbial culture fluid circuit enters the culture mode, the following steps are further included:

[0099] In the culture mode, the temperature in the culture tank 1 is adjusted using the temperature adjustment module according to the range of the microbial growth rate.

[0100] In this embodiment, if the growth rate is lower than the target range, the system will instruct the temperature control module to increase the temperature in the culture tank 1. In this way, increasing the temperature can promote the metabolic activity of the microorganisms, thereby accelerating growth; if the growth rate is within the target range, the system will maintain the current temperature setting to maintain stable growth of the microorganisms; if the growth rate exceeds the target range, the system may instruct the temperature control module to lower the temperature to slow down the growth rate of the microorganisms and prevent excessive growth.

[0101] In this way, by adjusting the temperature according to the growth rate, the system can optimize the growth conditions of microorganisms and ensure the accuracy and reproducibility of experimental results.

[0102] For example, after the culture begins, the temperature detection module 8 continuously monitors the temperature in the culture tank 1 to ensure that it remains within a suitable range for microbial growth (e.g., 37° C.). The growth rate monitoring module (e.g., an automatic imaging system) regularly records the growth of the microorganisms and calculates the growth rate.

[0103] At a certain stage in the experiment, monitoring data indicates that the microbial growth rate is lower than the target (for example, the target growth rate is 0.5% per hour, and the actual growth rate is 0.3%). After analyzing the growth rate data, the control system determines that the temperature needs to be increased to promote growth. The system instructs the temperature regulation module to gradually increase the temperature in culture tank 1 from 37°C to 38°C. This temperature increase is gradual to avoid heat stress on the microorganisms.

[0104] At the new temperature setting, the system continues to monitor the growth rate of the microorganisms. If the growth rate reaches or exceeds the target value, the system will maintain the current temperature. If the growth rate is still below the target, the system may further adjust the temperature or consider other factors that may affect the growth rate, such as nutrient availability.

[0105] According to some embodiments of the present invention, after the step of adjusting the operating parameters of the pump in real time according to the culture parameter information, the method for controlling the on-orbit microbial culture fluid circuit further includes:

[0106] When the culture time of the microorganisms in the culture tank 1 reaches the target culture period, the pump body is controlled to be closed.

[0107] The target training cycle is obtained as follows:

[0108] Obtaining the microbial species and target culture quantity of the microorganisms in the culture tank 1;

[0109] Calculate the total amount of culture fluid required for the microorganisms in culture tank 1 based on the microorganism species and target culture quantity;

[0110] The target culture period required for the microorganisms in the culture tank 1 is calculated based on the total amount of culture liquid and the power of the pump.

[0111] In this embodiment, the specific process for obtaining the target culture period is as follows: It will be appreciated that since each microorganism has its own specific growth rate and growth period, the total amount of culture medium required to achieve the target culture count for that type of microorganism can be estimated based on the growth characteristics of the microorganism. This typically requires reference to growth data for the microorganism under similar conditions or through preliminary laboratory experiments.

[0112] Furthermore, after obtaining the total amount of culture fluid required for microbial culture, the target culture cycle can be calculated based on the pump power. The target culture cycle is inversely correlated with the pump power, while it is positively correlated with the total amount of culture fluid.

[0113] In practical applications, the microbial growth rate and growth efficiency also need to be considered. Growth rate refers to the rate at which a microbial biomass increases per unit time, while growth efficiency refers to the microbial ability to convert nutrients into biomass. If the microbial growth rate is slow or the growth efficiency is low, it may take longer to reach the target culture count, requiring the target culture cycle to be adjusted accordingly.

[0114] Finally, in actual on-orbit experiments, it may be necessary to verify the accuracy of the calculated target culture period through experiments. This may involve conducting pilot experiments in a simulated microgravity environment on the ground, or conducting preliminary experiments on the space station.

[0115] The control device for an on-orbit microorganism culture fluid circuit provided by the present invention is described below. The control device for an on-orbit microorganism culture fluid circuit described below and the control method for an on-orbit microorganism culture fluid circuit described above can be referenced to each other.

[0116] like Figure 3As shown, the control device for the on-orbit microorganism culture fluid circuit according to the second embodiment of the present invention includes:

[0117] The first control module 110 is configured to receive a microorganism culture start signal and control the pump to start so that the on-track microorganism culture fluid path enters a culture mode;

[0118] The second control module 120 is used to monitor the culture parameter information in the culture tank 1 in the culture mode, and adjust the working parameters of the pump body in real time according to the culture parameter information.

[0119] Figure 4 An example of a physical structure diagram of an electronic device is shown below. Figure 4 As shown, the electronic device may include: a processor (Processor) 810, a communication interface (Communications Interface) 820, a memory (Memory) 830, and a communication bus 840, wherein the processor 810, the communication interface 820, and the memory 830 communicate with each other via the communication bus 840. The processor 810 can call the logic instructions in the memory 830 to execute the control method of the on-track microorganism culture fluid circuit, including: receiving a signal to start microorganism culture, controlling the pump body to start so that the on-track microorganism culture fluid circuit enters the culture mode; in the culture mode, monitoring the culture parameter information in the culture tank 1 and adjusting the operating parameters of the pump body in real time according to the culture parameter information.

[0120] In addition, the logic instructions in the above-mentioned memory 830 can be implemented in the form of a software functional unit and can be stored in a computer-readable storage medium when sold or used as an independent product. Based on this understanding, the technical solution of the present invention, or the part that contributes to the prior art, or the part of the technical solution, can be embodied in the form of a software product. The computer software product is stored in a storage medium and includes several instructions for enabling a computer device (which can be a personal computer, a server, or a network device, etc.) to perform all or part of the steps of the various embodiments of the present invention. The aforementioned storage medium includes: various media that can store program codes, such as a USB flash drive, a mobile hard disk, a read-only memory (ROM), a random access memory (RAM), a magnetic disk, or an optical disk.

[0121] On the other hand, the present invention also provides a computer program product, which includes a computer program. The computer program can be stored on a non-transitory computer-readable storage medium. When the computer program is executed by a processor, the computer can execute the control method of the on-orbit microbial culture fluid circuit provided by the above methods, including: receiving a signal to start microbial culture, controlling the pump body to start so that the on-orbit microbial culture fluid circuit enters the culture mode; in the culture mode, monitoring the culture parameter information in the culture tank 1, and adjusting the working parameters of the pump body in real time according to the culture parameter information.

[0122] On the other hand, the present invention also provides a non-transitory computer-readable storage medium having a computer program stored thereon. When the computer program is executed by a processor, it is implemented to execute the control method of the on-orbit microbial culture liquid circuit provided by the above-mentioned methods, including: receiving a signal to start microbial culture, controlling the pump body to start so that the on-orbit microbial culture liquid circuit enters the culture mode; in the culture mode, monitoring the culture parameter information in the culture tank 1, and adjusting the working parameters of the pump body in real time according to the culture parameter information.

[0123] The device embodiments described above are merely illustrative. The units described as separate components may or may not be physically separate, and the components shown as units may or may not be physical units. That is, they may be located in one place or distributed across multiple network units. Some or all of the modules may be selected based on actual needs to achieve the objectives of the present embodiment. Persons of ordinary skill in the art will be able to understand and implement the present invention without inventive effort.

[0124] Through the description of the above embodiments, those skilled in the art can clearly understand that each embodiment can be implemented by means of software plus the necessary general hardware platform, or of course, by hardware. Based on this understanding, the essence of the above technical solution or the part that contributes to the existing technology can be embodied in the form of a software product. The computer software product can be stored in a computer-readable storage medium, such as ROM / RAM, a magnetic disk, an optical disk, etc., and includes a number of instructions for causing a computer device (which can be a personal computer, a server, or a network device, etc.) to execute the methods of each embodiment or certain parts of the embodiment.

[0125] In the description of this specification, the reference terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0126] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. An on-orbit microbial culture fluid circuit, characterized in that: include: A culture tank having a liquid inlet and an air outlet, and the culture tank is used to provide a closed culture environment for microbial culture; A storage device is formed with a liquid storage cavity and an air storage cavity, wherein the liquid storage cavity and the air storage cavity are isolated from each other by a flip membrane, a liquid outlet is formed on the liquid storage cavity, and an air inlet is formed on the air storage cavity; The liquid storage chamber stores a culture solution for microbial culture, and the gas storage chamber is used to store gas; The reversible membrane is provided with a protrusion protruding toward the gas storage cavity; when the gas in the gas storage cavity presses the protrusion, it can be deformed and reversed, and completely sunken to one side of the liquid storage cavity; The liquid outlet of the liquid storage cavity is connected to the liquid inlet of the culture tank through a liquid pipeline, and the gas outlet of the culture tank is connected to the gas inlet of the gas storage cavity through a gas pipeline, wherein at least one of the liquid pipeline and the gas pipeline is provided with a pump body; During the microbial cultivation process, the liquid pipeline allows the culture liquid to flow from the storage device into the culture tank to provide necessary nutrients for the microorganisms, and the gas pipeline allows gas to be discharged from the culture tank and enter the gas storage cavity, thereby maintaining the pressure inside the culture tank.

2. The on-orbit microbial culture fluid circuit according to claim 1, characterized in that: A water pump is provided on the liquid pipeline.

3. The on-orbit microbial culture fluid circuit according to claim 2, characterized in that: A one-way valve is provided between the water pump and the liquid inlet of the culture tank, and the valve direction of the one-way valve is oriented along the direction of the water pump toward the liquid inlet.

4. The on-orbit microbial culture fluid circuit according to any one of claims 1 to 3, characterized in that: A temperature regulating module and a temperature detecting module are arranged outside the culture pool.

5. The on-orbit microbial culture fluid circuit according to any one of claims 1 to 3, characterized in that: The liquid pipeline is provided with a flow meter, and / or an air pressure detection module is provided outside the culture tank.

6. A method for controlling an on-orbit microbial culture fluid circuit according to any one of claims 1 to 5, characterized in that: include: Upon receiving a signal for starting microbial culture, the pump body is controlled to start so that the on-orbit microbial culture fluid path enters a culture mode; In the culture mode, the culture parameter information in the culture pool is monitored, and the working parameters of the pump body are adjusted in real time according to the culture parameter information.

7. The method for controlling an on-orbit microbial culture fluid circuit according to claim 6, characterized in that: In the step of monitoring the culture parameter information in the culture pool in the microorganism culture mode, the culture parameter information in the culture pool includes culture environment information and / or microorganism information in the culture pool.

8. The method for controlling an on-orbit microbial culture fluid circuit according to claim 7, characterized in that: The culture environment information includes the size of the air pressure in the pool, and the step of adjusting the working parameters of the pump body in real time according to the culture parameter information specifically includes: When the air pressure in the pool exceeds the maximum set air pressure, the power of the pump body is controlled to decrease until the air pressure in the pool is less than the maximum set air pressure; When the air pressure in the pool is lower than the minimum set air pressure, the power of the pump body is controlled to increase until the air pressure in the pool is greater than the minimum set air pressure.

9. The method for controlling an on-orbit microbial culture fluid circuit according to claim 7, characterized in that: The microbial information includes the microbial growth rate, and the step of adjusting the working parameters of the pump body in real time according to the culture parameter information specifically includes: When the microbial growth rate is lower than the target growth rate, the power of the pump is controlled to increase until the microbial growth rate reaches the target growth rate; When the growth rate of the microorganisms reaches or exceeds the target growth rate, the power of the pump body is controlled to remain unchanged.

10. The method for controlling an on-orbit microorganism culture fluid circuit according to any one of claims 7 to 9, characterized in that: The culture environment information includes the temperature in the pool, and the microbial information includes the microbial growth rate. After receiving the signal to start microbial culture, and controlling the pump to start so that the on-track microbial culture liquid circuit enters the culture mode, the method further includes: In the culture mode, the temperature in the culture tank is adjusted by the temperature adjustment module according to the range of the growth rate of the microorganisms.

11. The method for controlling an on-orbit microorganism culture fluid circuit according to any one of claims 7 to 9, characterized in that: After the step of adjusting the working parameters of the pump in real time according to the culture parameter information, the method further includes: When the culturing time of the microorganisms in the culturing tank reaches the target culturing period, controlling the pump body to shut down; The target cultivation period is obtained as follows: Obtaining the types of microorganisms in the culture tank and the target culture quantity of the microorganisms; Calculating the total amount of culture solution required for the microorganisms in the culture tank based on the microorganism species and the target culture quantity; The target culture period required for the microorganisms in the culture tank is calculated based on the total amount of the culture solution and the power of the pump.

12. A control device for an on-orbit microorganism culture fluid circuit according to any one of claims 1 to 5, characterized in that: include: a first control module, configured to receive a microorganism culture start signal and control the pump body to start so that the on-orbit microorganism culture liquid circuit enters a culture mode; The second control module is used to monitor the culture parameter information in the culture pool in the culture mode, and adjust the working parameters of the pump body in real time according to the culture parameter information.

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