A method of laser regulation of plant growth trends

By regulating plant growth with low-energy lasers and combining this with microclimate factors in photovoltaic agriculture, specific wavelengths and ratios of red, blue, and green lasers are used to supplement light, solving the problem of insufficient light caused by shading from photovoltaic panels and achieving efficient regulation of plant growth and enhanced nutritional value.

CN118592226BActive Publication Date: 2025-11-21ZHEJIANG CHANGXIN OPTOELECTRONIC TECH CO LTD

Patent Information

Application Number
CN202410700510.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Priority Date
2023-06-02
Filing Date
2024-05-31
Publication Date
2025-11-21
Estimated Expiration
2044-05-31

AI Technical Summary

Technical Problem

In photovoltaic agriculture, shading problems caused by photovoltaic panels lead to insufficient light inside the greenhouse or under the panels, affecting plant growth. Existing technologies mainly focus on supplemental lighting to solve the problem of insufficient light, but have failed to effectively coordinate the synergistic effects of microclimate environmental factors.

Method used

Low-energy lasers are used to regulate plant growth. By adjusting the light quality and intensity of the laser field, combined with microclimate factors such as ambient temperature, humidity, and light intensity, the growth process of plants can be regulated and promoted. Specific wavelengths and ratios of red, blue, and green lasers are used for supplemental lighting.

Benefits of technology

Under insufficient light conditions, it can effectively improve the growth efficiency and nutritional value of plants, synergistically regulate the influence of multiple microclimate environmental factors, and improve the planting effect under photovoltaics.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present application belongs to the field of agriculture and forestry planting, and particularly relates to a method for laser regulation and control of plant growth trend. The method comprises the following steps: 1) forming a uniform laser light field by arranging laser light sources in a certain array distribution; and 2) adjusting the light quality and intensity of the laser light field according to the influencing factors of the microclimate environment of the planting site; the influencing factors of the microclimate environment include environmental temperature, relative humidity and environmental light intensity. The present application realizes the combination of water, light and heat by coordinating the characteristics of planting under photovoltaics, and can realize the growth regulation and control of plants planted in the shed or under the board by combining the low-energy-consumption laser with the microenvironment characteristics in the shed or under the board, thereby effectively improving the planting effect.
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Description

TECHNICAL FIELD

[0001] The present application belongs to the field of agriculture and forestry planting, and particularly relates to a method for laser regulation of plant growth trend. BACKGROUND

[0002] In recent years, the construction of traditional photovoltaic power stations in near-load areas is faced with the situation of insufficient construction land. This provides an opportunity for the cross-border integration of the photovoltaic industry and agricultural planting. The combination of photovoltaic power generation and efficient agriculture realizes the cross-border integration of photovoltaic and agriculture, and photovoltaic agriculture emerges as the times require.

[0003] As a new cross-border business model, photovoltaic agriculture adopts different light transmittance photovoltaic panels or staggered laying, expands the gap between photovoltaic panel installation and deployment, and meets the sunlight demand of different plant photosynthesis to realize the double benefits of "shed roof power generation and shed planting". Through land transfer and other means, land resources are scaled and gathered, and in the case of not changing the nature and use of the land, photovoltaic power stations and agriculture realize facility sharing, cost sharing, and win-win situation of increasing yield and efficiency, which can solve the problem of insufficient land for photovoltaic power generation and crack the problem of agricultural land occupation, making it possible for photovoltaic power generation to develop on a large scale in population-dense areas in China.

[0004] However, while photovoltaic agriculture has obvious advantages, the problems are also obvious, and the most core problem is the shading problem of photovoltaic panels. The existing photovoltaic panels generally shade 30-90%, resulting in insufficient light in the shed or under the panel, affecting the growth of plants. At present, the problem is still generally solved by supplementing light to make plants obtain sufficient light to maintain their growth. For example, a kind of laser photovoltaic planting method CN116369083A researched by our company, but as the research deepens, our company finds that there is a very significant feature in planting under photovoltaic, that is, planting under photovoltaic is essentially a kind of reduced light planting in the shed or under the panel for plants, and it has two significant features: 1. Weak ambient light; 2. Stable environment in the shed or under the panel. At this time, it is believed that the main factor affecting plant growth is light, but our R&D personnel believe that insufficient light is one aspect, and more importantly, the coordination of light environment and other microclimate conditions factors affects the plant internal reaction process and efficiency. Therefore, in the process of in-depth research, the present application tends to coordinate the synergistic effect of various factors of the microclimate environment, and utilize the advantage of laser planting, i.e. high energy, to realize low-energy-consumption laser coordination with the microenvironment in the shed or under the panel to regulate plant growth, thereby maintaining efficient cultivation of plants under photovoltaic.

[0005] And on this basis, through more detailed research, the present application also extends the technology to a more extensive application field, so that it can be effectively applied not only to planting under photovoltaic, but also to almost all semi-open, closed or even insufficiently lighted open planting environments. Summary of the Invention

[0006] To address the problems of insufficient light, restricted or even stagnant plant growth, or death in many semi-open or closed planting conditions, and the fact that current research generally only addresses the superficial aspects of light, this invention provides a method for laser-controlled plant growth.

[0007] The main objective of this invention is:

[0008] First, it can ensure effective laser supplemental lighting to regulate plant growth with low-energy laser consumption;

[0009] II. To study the impact of microclimate on plant growth and to regulate and promote plant growth processes by using low-energy-consumption, high-energy-density lasers in conjunction with microclimate.

[0010] Third, it can coordinate the effects of multiple major microclimate environments on plants.

[0011] A method for controlling plant growth trends using lasers.

[0012] The method includes:

[0013] 1) Form a uniform laser light field by distributing the laser light source in a certain array;

[0014] In the laser source:

[0015] The center wavelength of the red laser is 645–665 nm;

[0016] The central wavelength of the blue laser is 435–455 nm;

[0017] The center wavelength of the green laser is 535–565 nm.

[0018] 2) Adjust the light quality and intensity of the laser field according to the influencing factors of the microclimate environment of the planting site;

[0019] The influencing factors of the microclimate environment include ambient temperature, relative humidity, and ambient light intensity.

[0020] As a preferred option

[0021] The influencing factors of the microclimate environment are determined as non-influencing factors, secondary influencing factors, or primary influencing factors based on the needs of the planted plants and the on-site test values.

[0022] As a preferred option

[0023] At the current ambient temperature T am If the plant can survive, combined with the ambient temperature T am, the minimum temperature for plant growth T min , the minimum temperature for plant growth T sui-min , the maximum temperature for plant growth T sui-max , and the maximum temperature for plant growth T max determination:

[0024] When T sui-min < T am < T sui-max , it is determined that the environmental temperature is a non-influencing factor at this time;

[0025] When T min < T am < T sui-min or T sui-max < T am < T max , it is determined that the environmental temperature is a secondary influencing factor;

[0026] When T am < T min or T max < T am , it is determined that the environmental temperature is a primary influencing factor;

[0027] The relative humidity RH am under which the plant can maintain survival, combined with the relative humidity RH am , the minimum humidity for plant growth RH min , the minimum humidity for plant growth RH sui-min , the maximum humidity for plant growth RH sui-max , and the maximum humidity for plant growth RH max determination:

[0028] When RH sui-min < RH am < RH sui-max , it is determined that the relative humidity is a non-influencing factor at this time;

[0029] When RH min < RH am < RH sui-min or RH sui-max < RH am < RH max , it is determined that the relative humidity is a secondary influencing factor;

[0030] When RH am < RH min or RH max < RH am , it is determined that the relative humidity is a primary influencing factor;

[0031] The current environmental light intensity L amWhen the environmental light intensity L am When the environmental light intensity L am When the environmental light intensity L

[0032] As a preferred,

[0033] After the three influencing factors of the microclimate environment are determined, when adjusting the laser light field, first, the suitable laser light quality and laser light intensity range are selected as the first range according to the main influencing factor, and then the suitable laser light quality and laser light intensity are selected as the second range according to the secondary influencing factor;

[0034] When there is only one first range and at least one second range: if there is an overlapping interval between the first range and the second range, the laser light quality and laser light intensity are set according to the overlapping interval; if there is no overlapping interval, the end value of the first range closest to the second range is taken;

[0035] When there are two first ranges: the overlapping interval of the two first ranges is taken, and the laser light quality and laser light intensity are set according to the overlapping interval; if there is no overlapping interval, the end value of any one of the two first ranges closest to the other first range is taken; if there is still a second range at this time, the end value of the first range closest to the other first range in the first range closest to or having the highest degree of overlap with the second range is taken;

[0036] When there is no first range and there are multiple second ranges: the overlapping interval of the second ranges is taken, and the laser light quality and laser light intensity are set according to the overlapping interval; if there is no overlapping interval, the end value of any one of the second ranges closest to the other second ranges is taken.

[0037] As a preferred,

[0038] When the environmental temperature satisfies T min <T am <T sui-min , red and blue laser light with a total light intensity of 0.5-3.5 μmol / (m 2 ·s) is used for light supplementing treatment, wherein the light intensity ratio of red laser light to blue laser light is (1.0-3.0):1;

[0039] When the environmental temperature satisfies T sui-max <T am <T max , red and blue laser light with a total light intensity of 0.5-2.0 μmol / (m 2 ·s) is used for light supplementing treatment, wherein the light intensity ratio of red laser light to blue laser light is 1:(1.0-3.0);

[0040] When the environmental temperature satisfies Tam <T min At that time, a total light intensity of 0.1–1.5 μmol / (m²) was used. 2 The red and blue lasers are used for supplementary lighting, wherein the intensity ratio of the red laser to the blue laser is (2.0~10.0):1;

[0041] The ambient temperature satisfies T max <T am At that time, a total light intensity of 0.1–0.5 μmol / (m²) was used. 2 The red and blue lasers are used for supplementary lighting, wherein the intensity ratio of the red laser to the blue laser is 1:(3.0~5.0).

[0042] The relative humidity satisfies RH min <RH am <RH sui-min At that time, a total light intensity of 0.5–3.0 μmol / (m²) was used. 2 The red and blue lasers are used for supplementary lighting, wherein the intensity ratio of the red laser to the blue laser is (2.0~5.0):1;

[0043] The relative humidity satisfies RH sui-max <RH am <RH max At that time, a total light intensity of 2.0–5.0 μmol / (m²) was used. 2 The red and blue lasers are used for supplementary lighting, wherein the intensity ratio of the red laser to the blue laser is 1:(1.0~3.0).

[0044] The relative humidity satisfies RH am <RH min At that time, a total light intensity of 0.1–1.0 μmol / (m²) was used. 2 The red and blue lasers are used for supplementary lighting, wherein the intensity ratio of the red laser to the blue laser is (3.0~10.0):1;

[0045] The relative humidity satisfies RH max <RH am At that time, a total light intensity of 0.2–1.5 μmol / (m²) was used. 2 The red and blue lasers are used for supplementary lighting, wherein the intensity ratio of the red laser to the blue laser is 1:(2.5~5.0).

[0046] The ambient light intensity L am When the light intensity is ≤L-PPFD, a total light intensity of 0.5~5.0μmol / (m²) is used. 2 The laser light is supplemented by a red laser (s), wherein the intensity ratio of the red laser to the blue laser is 1:(0.1~5.0).

[0047] As preferred,

[0048] According to the environmental condition setting the laser light field, the first first range is preferentially met with light quality as the main control parameter, and the end value is preferentially met with light quality light intensity ratio parameter meeting other first range and / or second range, and the end value light intensity of the first first range is taken, and adjustment is performed in cooperation with green light laser;

[0049] The green light laser and the red light laser are converted according to the light intensity ratio 1:0.80.

[0050] The green light laser and the blue light laser are converted according to the light intensity ratio 1:1.18.

[0051] In the technical scheme of the present application, the most important core is to first confirm how the plant develops under the influence of different microclimate environmental factors, and what kind of environmental inhibition effect the plant is subjected to when inhibited by the environment.

[0052] For this, the present application first selects three important microclimate environmental parameters that have an important influence on plant growth, temperature, humidity and light intensity, and the three factors are relatively constant factors in the microclimate environment, and the fluctuation rate is relatively low over time. In the research process of the present application, in order to improve the research and development efficiency, three economic plants with short growth cycle are selected as the research objects, namely: bean sprouts, leeks and fine onions.

[0053] In the microclimate environment, the first important environmental parameter is temperature, which has a wide range of tolerance growth temperature interval and a small range of suitable growth temperature interval for plant growth. In the two intervals, taking bean sprouts as an example, under the condition of suitable humidity (RH = 90%), the tolerance growth temperature interval of the selected bean sprout species in the development process of the application is about 10-32 DEG C, and the suitable growth temperature interval is 21-27 DEG C. In the interval of 21-27 DEG C, the bean sprouts grow faster and better, the general growth period is 6-7 days (harvest time, not maximum growth time), the length is 3-5 cm, and the nutritional value is relatively high. When the temperature drops to 10-21 DEG C, the growth rate decreases, the nutrient accumulation is slow, the growth period is extended to 8-11 days, and the length of the harvested bean sprouts is slightly shortened, between 2-5 cm, and the nutritional value is changed, slightly reduced in some nutrients. When the temperature rises to 27-30 DEG C, the growth rate of bean sprouts is significantly accelerated, and the growth period can be shortened to 5-6 days, but the nutrient accumulation is less, and the nutritional value is lower than that of 10-21 DEG C. With the temperature rising to 30-32 DEG C, not only the nutritional value decreases more, but also the rotten sprouts and other conditions are easily found. For this, the inventors of the application find that under the condition of low temperature, red and blue mixed laser irradiation and red laser as the relative main part can effectively promote the metabolic efficiency and effect of plants under relatively low temperature. For example, bean sprouts under the condition of 10-21 DEG C environment temperature, the respiration and photosynthesis are weakened, leading to the growth and development of bean sprouts to slow down, and even to stop. Laser has the characteristics of high energy density, high coherence and high penetration, which can more effectively penetrate the cells and affect the deep-acting organelles. As for the light quality studied at present, blue light has the relatively optimal effect because of its large energy density without causing damage to cells and organelles. The photosensitive organs of plants, such as cryptochrome, are generally sensitive to red and blue light. LED red light has the effect of promoting adventitious bud growth and inducing callus development, but the effect of laser is obviously different from that of incandescent light, LED light and other general light sources. The high energy, high penetration and high coherence of laser make it more directly and effectively act on deep cells. Red laser promotes the production of plant hormones, shortens the light reaction period, improves water use efficiency, and achieves the effect of inhibiting transpiration, so that the "heat dissipation" process of plants under low temperature is inhibited, the nutrient utilization rate is improved, and the overgrowth of plants is inhibited to some extent, so that the nutrient utilization efficiency is improved, the ability of nutrient and energy allocation is improved, the synthesis and enrichment of nutrients are improved, the influence of low temperature is weakened by internal reaction, and the content and activity of chlorophyll and other components are effectively maintained. Blue laser is beneficial to improve respiration and improve the internal reaction heat production capacity of plants. From the perspective of total light intensity,When the ambient temperature can still meet its tolerance growth range, a relatively large light intensity can be used, and when the actual temperature further decreases, such as the temperature of bean sprouts decreases to <10°C, in general, the survival rate of bean sprouts has decreased significantly, and in this case, the total light intensity is reduced, because the bean sprouts are very fragile at this time, and if a larger light intensity is used, it will easily lead to adverse effects, and a larger proportion of red light is needed at this time to enhance the red-blue laser cooperation effect, such as the research process of the present application found that the light intensity of the bean sprouts decreased significantly and some completely dried out, in this case, the proportion of red light in the red-blue laser needs to be further increased to improve the survival rate of bean sprouts, because a high proportion of red light can further improve the respiration / photosynthesis ratio excited by light, thereby adjusting and ensuring that bean sprouts have enough energy and nutrients to maintain survival and can grow at a relatively slow rate and accumulate nutrients. High temperature is the opposite, and high blue light and low red light mixed laser can inhibit respiration to some extent and speed up transpiration to cool down, thereby achieving self-control and speeding up energy consumption and cooling.

[0054] Humidity is another important environmental parameter for plants, and when the humidity is too high or too low, the stomata of the plants will close, affecting their respiration and photosynthesis. Taking bean sprouts as an example, the suitable relative humidity for bean sprouts is about RH=88-92%, and the tolerance relative humidity is generally in the range of RH=60-95%. When the relative humidity of the bean sprouts used in the present application is between 88-92%, the ambient temperature is 21-27°C, which is in the suitable range and is not considered an influencing factor. When the relative humidity decreases to 60-85%, a red-blue light mixture with slightly stronger red light is needed to stimulate the respiration of bean sprouts and / or form a certain degree of stomatal expansion, thereby making it easier to capture water vapor from the environment. When the humidity further decreases to RH < 60%, a lower blue light strategy is needed, because low blue light limits transpiration and respiration, thereby reducing water loss and inhibiting respiration to reduce the consumption of water for energy conversion and more inclined to synthesize effective nutrients, limiting stomatal closure and promoting stomatal expansion to some extent. Therefore, the stomata expanded by the enhanced transpiration are also beneficial to obtaining water from the environment, which has shown better results for humidity-sensitive plants such as bean sprouts. In the case of high humidity, high blue light is needed to enhance respiration and transpiration to avoid rotting and mold of bean sprouts.

[0055] Compared with the technical scheme, the environmental light intensity is a relatively less important influencing parameter, and for photovoltaic planting, basically due to the limited light transmittance of photovoltaic panels and / or photovoltaic films, the normal light requirement of plants cannot be met in the shed or under the panel, and many existing studies are in pursuit of sufficient light intensity for plants, but the present application is completely different, which directly intervenes in the internal reaction process of plants through extremely low energy consumption laser, so as to ensure the normal development of plants in the case of insufficient light and even more efficient development in combination with the microenvironment characteristics of the shed or under the panel, and therefore for the present application, only the light intensity L am whether L am ≤L-PPFD is met, that is, whether the light intensity in the shed or under the panel reaches the light compensation point (L-PPFD), because there is a certain difference in the influence of respiration and photosynthesis of plants before and after reaching the light compensation point, of course, the present technical scheme is macroscopically reflected in the influence of the photosynthesis-respiration ratio of plants under different microenvironment conditions, so it is not a major influencing factor, and when some plants need to be cultivated in the dark, such as bean sprouts (which can be cultivated in the dark or under light), the light absorption rate of photovoltaic panels and / or photovoltaic films can be increased to generate more power for photovoltaic power generation and further reduce the environmental light intensity in the shed or under the panel, so that the photovoltaic power generation has higher flexibility, and the light transmittance of the photovoltaic module is no longer an important factor affecting photovoltaic planting.

[0056] In addition, it is also necessary to note that the present application has also found that laser light compensation has a certain influence on the light compensation point of plants, which can reduce the light compensation point of plants, but at present, when the laser light with the three light qualities is used for compensation, the main laser parameter affecting the light compensation point is the appropriate light intensity ratio of the light quality, so when calculating the light compensation point L-PPFD of plants, the actual light compensation point (L-PPFD-real) of plants under the actual total light intensity of the laser used in the experiment under the condition of gradually reducing the environmental light intensity is not judged by the existing light compensation point, so as to ensure better results, if L am whether L am ≤L-PPFD-real is met through pre-experiment, the actual light compensation effect will be better and the actual effect on plants will be better.

[0057] The beneficial effects of the present application are:

[0058] The application realizes the combination of water, light and heat by coordinating the characteristics of photovoltaic planting, and can realize the growth regulation of the plants in the shed or under the board by the low-energy-consumption laser combined with the micro-environment characteristics in the shed or under the board, thereby effectively improving the planting effect. BRIEF DESCRIPTION OF DRAWINGS

[0059] Figure 1 It is the entity photo of the mung bean sprouts picked after the CK group;

[0060] Figure 2 It is the entity photo of the mung bean sprouts picked after the G1-1 and G1-2 experimental groups;

[0061] Figure 3 It is the entity photo of the mung bean sprouts picked after the CG1-1 experimental group;

[0062] Figure 4 It is the entity photo of the mung bean sprouts picked after the CG1-2 experimental group;

[0063] Figure 5 It is the entity photo of the grafted watermelon seedlings before transplanting;

[0064] Figure 6 It is the entity photo of the tomato love jolie experimental group when the fruit is swelling and turning color. DETAILED DESCRIPTION

[0065] The application will be further described and illustrated in detail in the following combined with specific embodiments and the drawings. The person skilled in the art can implement the application based on these descriptions. In addition, the embodiments of the application involved in the following description are generally only a part of the embodiments of the application, not all the embodiments. Therefore, all other embodiments obtained by the person skilled in the art based on the embodiments in the application without creative labor should belong to the protection scope of the application.

[0066] In the description of the application, it should be understood that the terms "thickness", "upper", "lower", "horizontal", "top", "bottom", "inner", "outer", "circumferential" and the like indicate the orientation or positional relationship shown in the drawings, which are only for the convenience of describing the application and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the application. In the description of the application, the meaning of "a plurality of" is at least two, such as two, three, etc., and the meaning of "several" is one or more, unless otherwise explicitly and specifically limited.

[0067] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting", "fixing" and the like should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected or in communication with each other; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0068] Unless otherwise specified, the raw materials used in the embodiments of the present application are commercially available or available to those skilled in the art. Unless otherwise specified, the methods used in the embodiments of the present application are methods mastered by those skilled in the art.

[0069] Unless otherwise specified, in the laser light source used in the present application, the central wavelength of the red laser is 650 nm, the central wavelength of the blue laser is 445 nm, and the central wavelength of the green laser is 550 nm.

[0070] Example 1

[0071] A method for regulating the growth trend of plants by laser, the cultivation object used in this example is bean sprouts (tolerance temperature 10-32℃, suitable temperature 21-27℃, tolerance humidity RH=60-95%, suitable humidity RH=88-92%, light compensation point L-PPFD about 220lux, harvesting period 6-7d), cultivation is indoor cultivation (under photovoltaic panel, sealed shed), the method comprises:

[0072] 1) Distribute the laser light source according to a certain array to form a uniform laser light field, so that the irradiation intensity of the plant planting area is maintained at an average, and the absolute value of the difference between the minimum value and the maximum value of the light compensation laser intensity in the planting area is ≤2% of the minimum value of the light intensity;

[0073] 2) Adjust the light quality and intensity of the laser light field according to the influencing factors of the microclimate environment of the planting area;

[0074] The influencing factors of the microclimate environment include environmental temperature, relative humidity and environmental light intensity;

[0075] The current environmental temperature T am = 17℃, the current relative humidity RH am = 76%, and the environmental light intensity is about 6720lux;

[0076] After judgment:

[0077] The above environmental temperature condition satisfies T min <T am <T sui-minTherefore, the ambient temperature is a secondary influencing factor;

[0078] The above relative humidity condition satisfies RH min <RH am <RH sui-min Therefore, the relative humidity is a secondary influencing factor;

[0079] The above ambient light intensity satisfies L am ≥L-PPFD, therefore the ambient light intensity is a non-influencing factor;

[0080] Based on the above determination results, the following range intervals are formed:

[0081] The ambient temperature forms a second range interval: a second range interval in which the light intensity ratio of red laser and blue laser is (1.0-3.0):1 and the total light intensity is 0.5-3.5 μmol / (m 2 ·s);

[0082] The relative humidity forms a second range interval: a second range interval in which the light intensity ratio of red laser and blue laser is (2.0-5.0):1 and the total light intensity is 0.5-3.0 μmol / (m 2 ·s);

[0083] The ambient light intensity does not form a second range interval;

[0084] Based on the above determination results, and the interval selection criteria, the present application respectively selects red laser and blue laser light intensity ratio of 1.0:1 and total light intensity of 2.5 μmol / (m 2 ·s) red-blue laser mixed light for light supplementing treatment as an experimental group G1-1, selects red laser and blue laser light intensity ratio of 2.5:1 and total light intensity of 2.5 μmol / (m 2 ·s) red-blue laser mixed light for light supplementing treatment as an experimental group G1-2, and an experimental group grown under suitable conditions (ambient temperature 25℃, relative humidity RH=90%, ambient light intensity about 6700 lux) as a blank control CK, an experimental group without light supplementing as a control group CG1-1, a red-blue LED light supplementing lamp with red-blue light intensity ratio of 1:1.0 and total light intensity of 2.5 μmol / (m 2 ·s) for light supplementing treatment as a control group CG1-2, and a red-blue LED light supplementing lamp with red-blue light intensity ratio of 1:1.0 and total light intensity of 280 μmol / (m 2 ·s) for light supplementing treatment as a control group CG1-3;

[0085] After 7d cultivation and harvesting, the harvested bean sprouts are characterized for physical and chemical properties. The data recorded during the experiment are summarized as shown in the following table.

[0086] CK G1-1 G1-2 CG1-1 CG1-2 CG1-3 Sprout average length (mm) 59 57 59 49 50 55 Water content (g / 100g) 91.8 90.6 92.0 89.6 89.5 90.2 Protein content (g / 100g) 3.1 3.3 3.1 2.8 2.9 2.9 Carbohydrate content (g / 100g) 2.7 2.9 2.6 2.2 2.1 2.3 Insoluble dietary fiber content (g / 100g) 0.9 1.0 1.0 3.7 3.6 2.8 Total FFA content (g / 100g dry matter) 13.1 11.3 14.8 8.6 8.5 9.7

[0087] Note: g / 100g in the table represents water content, g / 100g dry matter represents dry matter content.

[0088] Among them, the solid photos of mung bean sprouts after picking in the CK group are as shown in Figure 1 The solid photos of mung bean sprouts after picking in the G1-1 and G1-2 experimental groups are as shown in Figure 2 The left two in the figure are the G1-1 experimental group, and the right three are the G1-2 experimental group, the solid photos of mung bean sprouts after picking in the CG1-1 experimental group are as shown in Figure 3 The solid photos of mung bean sprouts after picking in the CG1-2 experimental group are as shown in Figure 4 .

[0089] From the above characterization results and solid photos Figures 1-4 It can be seen that under the condition that temperature and humidity are insufficient to meet the optimal growth conditions of bean sprouts, the development of bean sprouts is actually affected very obviously. First, as in the CG1-1, CG1-2 experimental groups and Figures 3-4As shown, the bean sprouts are significantly shortened, and the root curling is very obvious. In terms of nutrition, the bean sprouts have relatively serious water loss, resulting in a significant decrease in water content, and the synthesis and retention of nutrients are also significantly hindered. In order to adapt to the low-temperature environment, plants will increase the synthesis of insoluble cellulose to enhance the stability and cold resistance of cell walls. Although the metabolic activity of the plant body will slow down under low temperature and low humidity, the activity of cellulose synthase may still remain at a high level, resulting in a relatively increased rate of cellulose synthesis, so that the insoluble dietary fiber content is obviously increased. In fact, for the overall nutritional content of the plant, the fresh weight protein content and the carbohydrate content have decreased, and due to the increase in insoluble dietary fiber content, the decrease in dry weight content of protein and carbohydrate is greater than the results shown in the table above, which has a huge impact on the nutritional value of bean sprouts. On the other hand, for food crops, the total FFA dry matter content also decreases significantly, by as much as 35% or more. It can be seen that the unsuitable growth environment has a destructive effect on the synthesis of nutrients in bean sprouts, and it can be seen that under the effect of weak white light (G1-2 experimental group), the actual growth conditions have not improved, indicating that low-intensity white light has little effect on regulating plant growth. For the G1-1, G1-2 and CG1-3 experimental groups, compared with the CG1-1 experimental group, obvious gain effect is produced. Although the water content of the CG1-1 experimental group has decreased, the actual protein content and carbohydrate content have increased, and although the insoluble dietary fiber content has also increased slightly, the overall quality is very close to that of the CK control group. Especially for the G1-2 experimental group, although the protein content and carbohydrate content are lower than those of the G1-1 experimental group, the actual fresh weight content of the total FFA content is much higher than that of the CG1-1 experimental group, even higher than that of the G1-1 experimental group. It can be seen that different light quality has a significant difference in the regulation of plant growth in adverse environments. The CG1-3 experimental group uses LED light with a intensity ten times higher than that of G1-1 and G1-2, and uses the same light quality as the G1-1 experimental group, but the results are very different. The form of light supplementation in the CG1-3 experimental group simply provides more energy for the growth of bean sprouts, but this energy is still limited for the synthesis of effective nutrients and respiration in order for the plant to adapt to the adverse environment. The LED red light itself has the effect of inducing callus development, resulting in the synthesis of more insoluble dietary fiber in order to resist the adverse environment, which also leads to the fact that although it can enhance the self-resistance of plants to the adverse environment to a certain extent, it cannot obtain excellent cultivation results.

[0090] Moreover, on this basis, the wavelength of the red and blue laser used in the research and development process of the application is verified by test, because the wavelength of light has a close relationship with its penetration and energy density, and therefore according to the research results at the present stage, not all red light with a wavelength of 622-760 nm can produce obvious effects, and the effect is more remarkable when red light laser with a wavelength of 645-665 nm is used, and similarly, not all blue light laser with a wavelength of 400-500 nm can produce corresponding effects, but the blue light laser with a wavelength of 435-455 nm is preferably selected, and the effect of the red light laser with a wavelength of 645-665 nm is better.

[0091] Example 2

[0092] Further based on Example 1, different microclimate environments are constructed in the photovoltaic shed to verify the growth regulation effect of different light supplement conditions on plants in different microclimate environments.

[0093] In order to save space and form a more direct contrast effect, the examples and subsequent examples and comparative examples are only judged by the length of the entity (unit: mm), fresh water content (g / 100g) and dry weight relative nutritional value (score system) to judge the cultivation effect, wherein the dry weight relative nutritional value is calculated by the following form, and the total score is 90 points, wherein the dry matter content of protein, carbohydrate and FFA is 30 points respectively, and the CK experimental group (Example 1, the same below) is taken as a control, if the content of several substances is less than or equal to the corresponding dry matter content of the CK group, the score = (actual dry matter content average / CK group corresponding dry matter content) x 30, if the content of the substance is greater than the corresponding dry matter content of the CK group, it is marked as 30 points and marked with an asterisk, i.e. in Example 1, the G1-1 experimental group, wherein the protein score and the carbohydrate score are both 30, and the FFA score is 29.7, and since the protein and carbohydrate contents are greater than the corresponding dry matter content of the CK group, the dry weight relative nutritional value score is 89.7**.

[0094] Microclimate environment A:

[0095] The influencing factors of the microclimate environment include environmental temperature, relative humidity and environmental light intensity;

[0096] The current environmental temperature T am = 19℃, the current relative humidity RH am = 55%, and the environmental light intensity is about 6720 lux;

[0097] After judgment:

[0098] The above environmental temperature condition satisfies T am < T min , so the environmental temperature is the main influencing factor;

[0099] The above relative humidity condition satisfies RH min < RH am < RH sui-min Therefore, the relative humidity is a secondary influencing factor;

[0100] The above ambient light intensity satisfies L am ≥ L-PPFD, therefore the ambient light intensity is a non-influencing factor;

[0101] Based on the above determination results, the following range intervals are formed:

[0102] The ambient temperature forms a first range interval: a first range interval in which the intensity ratio of red laser light and blue laser light is (2.0-10.0): 1 and the total light intensity is 0.1-1.5 μmol / (m 2 ·s);

[0103] The relative humidity forms a second range interval: a second range interval in which the intensity ratio of red laser light and blue laser light is (2.0-5.0): 1 and the total light intensity is 0.5-3.0 μmol / (m 2 ·s);

[0104] The ambient light intensity does not form a second range interval;

[0105] Based on the above determination results, and the interval selection criteria, the optimal effective interval is:

[0106] The intensity ratio of red laser light and blue laser light is (2.0-5.0): 1 and the total light intensity is 0.5-1.5 μmol / (m 2 ·s), that is, the overlapping interval of the above first range interval and the second range interval.

[0107] Thus, this example sets up a single-factor variable experiment series, in which in the G2-1 series experiment group, the intensity ratio of red laser light and blue laser light is taken as the variable, and the total light intensity is controlled to always remain 1.0 μmol / (m 2 ·s), and the same 7d cultivation results as in Example 1 are shown in the following table.

[0108] RB ratio (red to blue laser intensity ratio) 1:1 2:1 3:1 4:1 5:1 7:1 10:1 12:1 Physical average length (mm) 52 57 56 57 55 56 53 49 Fresh weight water content (g / 100g) 87.3 89.1 90.1 89.6 89.3 88.9 89.0 88.2 Dry weight relative nutritional value degree 82.1 87.6* 87.9* 88.3* 89.0** 86.3* 83.7 71.1*

[0109] From the results of the above table, when the RB ratio is (2.0-5.0):1, the laser light supplementing effect is obviously better than that of other RB ratios, and when the RB ratio is 1:1, the average length and fresh weight water content decrease significantly and approach the minimum value, especially the fresh weight water content, which also shows that high blue light proportion laser light supplementing will trigger the internal reaction of the plant to cause the water consumption of the plant to increase or the water retention capacity to weaken, thereby causing the water content to decrease significantly, and with the relative proportion of red light laser increasing, the average length and fresh weight water content of the entity both basically show a trend of first increasing and then decreasing, and the trend of the average length is more significant, while the change of the fresh weight water content is relatively small, so it can be seen that red light has a good effect on water retention of the plant in the extremely low temperature and low humidity environment. However, the data in the table is relatively special, that is, when the RB ratio is 12:1, the dry weight relative nutritional value is 71.1, which indicates that one of the nutrient components is even higher than that of the CK group. Actually, from the original data, it can be seen that the FFA dry matter content in the experimental group with an RB value of 12:1 reaches 13.2 g / 100 g of dry matter, and the FFA dry matter content in the experimental group with an RB value of 10:1 also reaches 13.0 g / 100 g of dry matter, so it can be seen that relatively high-intensity red light laser has a relatively obvious effect on the accumulation of FFA in plants.

[0110] Further, the G2-2 series experimental groups are set, the total light intensity is taken as a variable, and the RB value is controlled to be 2.0:1, and the same 7d cultivation as in Example 1 is carried out, and the results are shown in the following table.

[0111] Total light intensity (pmol m -2 ·s -1 )]]> 0.1 0.5 1.0 1.5 2.0 2.5 3.0 3.5 Physical average length (mm) 56 58 57 57 55 53 46 39 Fresh weight water content (g / 100g) 87.6 88.6 89.1 89.1 89.0 89.3 90.9 92.2 Dry weight relative nutritional value degree 83.1* 86.9* 87.6* 88.1* 85.2* 81.3* 73.2 57.7

[0112] From the results of the above table, in the case of low total light intensity, it can be seen that the plants all show good coordinated growth, and with the total light intensity gradually increasing, the fresh weight water content first shows a slow increase and then an explosive increase in the case of high-intensity red light, and the dry weight relative nutritional value score also rapidly decreases accordingly, so it can be seen that appropriate light intensity ratio and appropriate light quality ratio can play a good growth regulation role, especially in combination with the G2-1 and G2-2 series experimental groups, it can be seen that according to the selection criteria of the present application, the light intensity ratio of red laser and blue laser is (2.0-5.0):1 and the total light intensity is 0.5-1.5 μmol / (m 2 ·s) as the laser light supplementing parameter interval, good light supplementing and growth regulation effects can be achieved, but the microclimate environment A in this example is in an extremely low temperature environment, which is lower than the minimum tolerance growth temperature of the bean sprouts, so if a laser with too high intensity is used, such as 3.5 μmol / (m 2The total light intensity of s) is far higher than that of other groups, and the fresh weight water content is far lower than that of other groups, and the length and dry weight of the entity are far lower than the relative nutritional value of other groups. Although high light intensity is beneficial to providing more energy / heat to the plant, due to the characteristics of laser, the actual high coherence and penetration can easily cause serious and irreversible damage to the plant itself which is fragile. Under the action of high light intensity laser, the internal reaction activity of the actual plant is increased, and the water absorption and transportation system is also enhanced, but the extreme low temperature condition inhibits the material synthesis process, and affects the internal and external osmotic pressure of the plant cell, so that the phenomenon of rapid increase of water and rapid decrease of nutrient content appears. Therefore, for the laser light supplementing of the plant-environment coordination, the light supplementing laser is more important than "moderate" rather than the higher the better.

[0113] Microclimate environment B:

[0114] The influencing factors of the microclimate environment include environmental temperature, relative humidity and environmental light intensity;

[0115] The current environmental temperature T am = 18℃, the current relative humidity RH am = 55%, and the environmental light intensity is about 6720 lux;

[0116] After judgment:

[0117] The above environmental temperature condition satisfies T min <T am <T sui-min , so the environmental temperature is a secondary influencing factor;

[0118] The above relative humidity condition satisfies RH am <RH min , so the relative humidity is a main influencing factor;

[0119] The above environmental light intensity satisfies L am ≥ L-PPFD, so the environmental light intensity is a non-influencing factor;

[0120] Based on the above judgment results, the following range intervals are formed:

[0121] The environmental temperature forms a second range interval: the light intensity ratio of red laser and blue laser is (1.0-3.0):1 and the total light intensity is 0.5-3.5 μmol / (m 2 ·s) of the second range interval;

[0122] The relative humidity forms a first range interval: the light intensity ratio of red laser and blue laser is (3.0-10.0):1 and the total light intensity is 0.1-1.0 μmol / (m 2 ·s) of the first range interval;

[0123] The environmental light intensity does not form the second range interval;

[0124] Based on the above determination results, and interval selection criteria, the optimal effective interval is:

[0125] The red laser and blue laser light intensity ratio is 3.0:1 and the total light intensity is 0.5-1.0 μmol / (m 2 ·s), that is, the overlap interval of the above first range interval and the second range interval.

[0126] In this example, a single factor variable experiment series is set. In the G2-3 series experiment group, the red laser and blue laser light intensity ratio is taken as the variable, and the total light intensity is controlled to always remain 0.75 μmol / (m 2 ·s), and the same 7d cultivation results as in Example 1 are shown in the following table.

[0127] RB ratio (red to blue laser intensity ratio) 1.5:1 2.0:1 2.5:1 3.0:1 3.5:1 4.0:1 Physical average length (mm) 51 52 56 58 57 57 Fresh weight water content (g / 100g) 86.1 86.3 86.8 87.6 86.1 85.9 Dry weight relative nutritional value degree 73.2 75.7 81.2 85.9* 83.1 82.7*

[0128] From the above table results, combined with the results of the G2-1 and G2-2 series experiment groups, it can be seen that the parameter influence of the first range interval is obviously greater than that of the second range interval. For example, the bean sprout quality of the RB ratio of (1.5-2.5):1 is much worse than that of the experiment group with a red and blue laser light intensity ratio of (3.0-4.0):1. As mentioned earlier, in the case of extremely low humidity and dryness and relatively low temperature, a very high red light intensity ratio is required to improve the plant's water utilization rate and retention rate, improve the fresh weight water content of the bean sprouts, and improve the water utilization rate and retention rate, which also directly affects the synthesis and retention of nutrients by the plant in the extremely small climate environment.

[0129] Further, the total light intensity is taken as the variable as the G2-4 experiment group, and the RB value is controlled to be 3.0:1, and the same 7d cultivation results as in Example 1 are shown in the following table.

[0130] Total light intensity (pmol m -2 ·s -1 )]]> 0.25 0.50 0.75 1.00 1.25 1.50 Physical average length (mm) 57 58 58 57 55 50 Fresh weight water content (g / 100g) 87.0 87.2 87.6 87.1 86.2 82.1 Dry weight relative nutritional value degree 82.6* 86.1* 85.9* 85.5* 83.6 76.7

[0131] From the above table results, the conclusion of the G2-2 series experiment group is also confirmed. For extremely extreme environments, light intensity has a more direct and significant impact on plants. Because in extremely extreme conditions, the plant itself is very fragile, and excessive light intensity will cause the laser light supplement to have a beneficial effect on the plant while also causing some harm. For example, in the case of extremely low humidity and dryness, excessive light intensity will cause the plant to dehydrate and rot. This is different from the case of extremely low temperature, because the impact of extremely low temperature and low humidity on plants is not the same, and therefore the laser light supplement treatment conditions need to be adjusted appropriately according to the plant's internal reaction characteristics.

[0132] Example 3

[0133] Further based on the embodiment 1, different microclimate environments are built in the photovoltaic shed to verify the growth regulation effect of different light supplement conditions on plants in different microclimate environments.

[0134] Microclimate environment A:

[0135] The influencing factors of the microclimate environment include environmental temperature, relative humidity and environmental light intensity;

[0136] The current environmental temperature T am = 30℃, the current relative humidity RH am = 93%, and the environmental light intensity is about 6720 lux;

[0137] Judgment:

[0138] The above environmental temperature condition satisfies T sui-max <T am <T max , so the environmental temperature is a secondary influencing factor;

[0139] The above relative humidity condition satisfies RH sui-max <RH am <RH max , so the relative humidity is a secondary influencing factor;

[0140] The above environmental light intensity satisfies L am ≥ L-PPFD, so the environmental light intensity is a non-influencing factor;

[0141] Based on the above judgment results, the following range intervals are formed:

[0142] The environmental temperature forms a second range interval: the light intensity ratio of red laser and blue laser is 1:(1.0-3.0) and the total light intensity is 0.5-2.0 μmol / (m 2 ·s) of the second range interval;

[0143] The relative humidity forms a second range interval: the light intensity ratio of red laser and blue laser is 1:(1.0-3.0) and the total light intensity is 2.0-5.0 μmol / (m 2 ·s) of the second range interval;

[0144] The environmental light intensity does not form a second range interval;

[0145] Based on the above determination results, and the interval selection criteria, the optimal effective interval is:

[0146] The light intensity ratio of red laser and blue laser is 1:(1.0-3.0) and the total light intensity is 2.0 μmol / (m 2 ·s), which is the overlapping range of the above intervals.

[0147] Set single factor variable experiment series, in G3-1 series experiment group, when the light intensity ratio of red laser and blue laser is taken as a variable and the total light intensity is controlled to remain 2.0 μmol / (m 2 ·s) at all times, the RB value is controlled to remain 1:2.0 when the total light intensity is taken as a variable, and the same 7d cultivation as in Example 1 is carried out, and the results are shown in the following table.

[0148] RB ratio (red to blue laser intensity ratio) 1:1.25 1:1.5 1:2.0 1:2.25 1:2.5 Physical average length (mm) 53 57 58 58 57 Fresh weight water content (g / 100g) 90.6 90.8 91.0 91.1 90.6 Dry weight relative nutritional value degree 83.7 85.6 86.9* 85.8 85.3 Total light intensity (pmol m -2 ·s -1 )]]> 1.0 1.5 2.0 2.5 3.0 Physical average length (mm) 52 53 58 56 53 Fresh weight water content (g / 100g) 90.1 90.6 91.0 90.2 87.3 Dry weight relative nutritional value degree 83.1 85.6 86.9* 83.9 81.2

[0149] From the results in the above table, under the relatively high temperature and high humidity conditions, the general effect of laser light supplementation is relatively weaker than that of light supplementation under relatively low temperature and low humidity conditions. This may be that the study has not yet obtained the optimal light supplementation conditions, or it may be that under high temperature and high humidity conditions, the effects of water loss, transpiration and respiration are enhanced, and the accumulation of nutrients and the retention of water in plants are directly affected. As for the fresh weight water content, the influence of the RB ratio is relatively small compared to the total light intensity, because under the condition that the RB ratio does not change much, the plant changes the photosynthesis-respiration ratio and the internal reaction process, and the absorption, utilization and loss of water are still in a relatively balanced state. However, if the total light intensity is too high, the effect will be further enhanced, which will amplify the photosynthesis-respiration ratio and change the osmotic pressure of plant cells to some extent, so that the fresh weight water content will decrease significantly under high humidity conditions, especially under high light intensity conditions. As for the synthesis and retention of nutrients, the influence of the total light intensity is also significantly greater than that of the RB ratio, which is also due to the blockage of nutrients caused by water loss.

[0150] Microclimate environment B:

[0151] The influencing factors of the microclimate environment include environmental temperature, relative humidity and environmental light intensity;

[0152] The current environmental temperature T am = 35℃, the current relative humidity RH am = 93%, and the environmental light intensity is about 6720 lux;

[0153] After judgment:

[0154] The above environmental temperature condition satisfies T max <T am , so the environmental temperature is the main influencing factor;

[0155] The above relative humidity condition satisfies RH sui-max <RH am <RH max , so the relative humidity is a secondary influencing factor;

[0156] The ambient light intensity satisfies L am ≥ L-PPFD, so the ambient light intensity is a non-influencing factor;

[0157] Based on the above determination results, the following range intervals are formed:

[0158] The ambient temperature forms a first range interval: a light intensity ratio of red laser and blue laser of 1:(3.0-5.0) and a total light intensity of 0.1-0.5 μmol / (m 2 ·s) in the first range interval;

[0159] The relative humidity forms a second range interval: a light intensity ratio of red laser and blue laser of 1:(1.0-3.0) and a total light intensity of 2.0-5.0 μmol / (m 2 ·s) in the second range interval;

[0160] The ambient light intensity does not form the second range interval;

[0161] Based on the above determination results, and the interval selection criteria, the optimal effective interval is:

[0162] A light intensity ratio of red laser and blue laser of 1:3.0 and a total light intensity of 0.5 μmol / (m 2 ·s), that is, the first range interval is closest to the end value of the second range interval.

[0163] A single-factor variable experiment series is set, in the G3-2 series experiment group, when the light intensity ratio of red laser and blue laser is used as a variable and the total light intensity is controlled to remain 0.5 μmol / (m 2 ·s), when the total light intensity is used as a variable, the RB value is controlled to remain 1:3.0, the same 7d cultivation as in Example 1 is performed, and the results are shown in the following table.

[0164] RB ratio (red to blue laser intensity ratio) 1:2.0 1:2.5 1:3.0 1:3.5 1:4.0 Physical average length (mm) 56 57 59 58 57 Fresh weight water content (g / 100g) 87.6 88.3 89.1 88.7 87.9 Dry weight relative nutritional value degree 83.5 83.9* 85.9* 84.1 83.2 Total light intensity (pmol m -2 ·s -1 )]]> 0.1 0.3 0.5 1.0 2.0 Physical average length (mm) 55 58 59 56 43 Fresh weight water content (g / 100g) 83.3 85.6 89.1 86.1 81.0 Dry weight relative nutritional value degree 79.5 82.1 85.9* 81.6 77.7

[0165] From the results of the above table, in the environment of extremely high temperature and relatively high humidity, the phenomenon of the G3-1 experimental group is further amplified, and the total light intensity has a more significant effect on plants than the RB ratio. When the total light intensity is too low or too high, it will cause a significant decrease in dry weight relative nutritional value. This is mainly due to the effect of low light intensity on the relatively high humidity condition, especially in this case, the first range interval end value light intensity is used for adjustment, which is farther than the second range interval. In the case of high light intensity, the effect is the weakest because the plant loses water severely under high temperature conditions, the internal reaction is active but not balanced. In addition, the use of high light intensity can meet the adjustment needs of plants in the relatively high humidity microclimate environment, but it is far higher than the adjustment needs of plants under extreme high temperature conditions, which leads to a serious imbalance in internal reaction and a very serious water loss.

[0166] On the basis of the above experimental groups, the RB value is kept at 1:3.0, and further 0.5 μmol / (m 2 ·s) red and blue mixed laser is used to cooperate with 1.5 μmol / (m 2 ·s) green laser to cultivate bean sprouts under the same conditions. The cultivation results show that the average length of bean sprouts is 58 mm, the fresh weight water content is 89.3 g / 100 g, and the dry weight relative nutritional value is even as high as 86.2*. This shows that green laser will not produce adverse effects on plants as red and blue light does. Instead, when there is a large difference in light intensity between the first and second range intervals, green laser can be used to supplement relatively mild and less side effects of light intensity, thereby further optimizing the effect of laser light supplement.

[0167] Similarly, on the basis of the above experimental groups, 0.5 μmol / (m 2 ·s) red and blue mixed laser (RB ratio 1:4) is used to cooperate with 0.08 μmol / (m 2The green laser of s) is used to cultivate bean sprouts under the same condition, and the cultivation results show that the average length of bean sprout entity reaches 57mm, the fresh weight water content reaches 88.6g / 100g, and the relative nutritional value of dry weight is even as high as 84.3. In view of the effect, it can be seen that the green laser still has a certain role in regulating balance under the condition of imbalance of red and blue light ratio (RB ratio), but the actual effect is much weaker than the direct effect of red and blue laser. In combination with the foregoing green laser light compensation, the green laser light compensation has the effect of repairing the RB ratio, but due to its weak effect and weak side effect, the RB ratio imbalance can be avoided in actual use. After the green laser and the red laser are converted according to the light intensity ratio of 1:0.80, and the green laser and the blue laser are converted according to the light intensity ratio of 1:1.18, the RB ratio is actually repaired from 1:4 to 1:3, and the balance of the foregoing RB ratio 1:3 is not destroyed.

[0168] In the above-mentioned test, the researchers further verified the effective wavelength range of the green laser. The results show that not all green lasers in the wavelength range of 492-577nm can produce the corresponding effect of repairing imbalance, and the effect is better when the green laser with a wavelength of 535-565nm is used. Because the green laser with too small wavelength has weak penetration and weak imbalance repairing ability, it is easy to cause side effects. Although the green laser with too long wavelength has strong penetration and low energy, it does not have negative effects, but because the energy density is too low, its effect of repairing imbalance is also relatively limited.

[0169] Microclimate environment C:

[0170] The influencing factors of the microclimate environment include environmental temperature, relative humidity and environmental light intensity;

[0171] The current environmental temperature T am = 30℃, the current relative humidity RH am = 96%, and the environmental light intensity is about 6720lux;

[0172] After judgment:

[0173] The above environmental temperature condition satisfies T sui-max <T am <T max , so the environmental temperature is a secondary influencing factor;

[0174] The above relative humidity condition satisfies RH max <RH am , so the relative humidity is a main influencing factor;

[0175] The above environmental light intensity satisfies L am ≥ L-PPFD, so the environmental light intensity is a non-influencing factor;

[0176] Based on the above determination results, the following range intervals are formed:

[0177] The ambient temperature forms a second range interval: the light intensity ratio of red laser and blue laser is 1:(1.0-3.0) and the total light intensity is 0.5-2.0 μmol / (m 2 ·s) of the second range interval;

[0178] The relative humidity forms a first range interval: the light intensity ratio of red laser and blue laser is 1:(2.5-5.0) and the total light intensity is 0.2-1.5 μmol / (m 2 ·s) of the first range interval;

[0179] The ambient light intensity does not form a second range interval;

[0180] Based on the above determination results, and the interval selection criteria, the optimal effective interval is:

[0181] The light intensity ratio of red laser and blue laser is 1:(2.5-3.0) and the total light intensity is 0.5-1.5 μmol / (m 2 ·s), that is, the overlapping range of the above intervals.

[0182] A single-factor variable experiment series is set, in the G3-3 series experiment group, when the light intensity ratio of red laser and blue laser is used as a variable and the total light intensity is controlled to remain 1.0 μmol / (m 2 ·s), when the total light intensity is used as a variable and the RB value is controlled to remain 1:2.5, the same 7d cultivation as in Example 1 is carried out, and the results are shown in the following table.

[0183] RB ratio (red to blue laser intensity ratio) 1:2.0 1:2.5 1:3.0 1:3.5 1:4.0 Physical average length (mm) 58 59 58 59 58 Fresh weight water content (g / 100g) 91.9 91.1 90.9 90.7 90.8 Dry weight relative nutritional value degree 83.9 88.6** 87.9** 85.2 84.7* Total light intensity (pmol m -2 ·s -1 )]]> 0.2 0.5 1.0 1.5 2.0 Physical average length (mm) 59 58 59 57 58 Fresh weight water content (g / 100g) 90.5 90.8 91.1 91.0 90.1 Dry weight relative nutritional value degree RB ratio (red to blue laser intensity ratio) Physical average length (mm) Fresh weight water content (g / 100g) Dry weight relative nutritional value degree Physical average length (mm) Fresh weight water content (g / 100g) Dry weight relative nutritional value degree 87.2* 88.1** 88.6** 88.2** 85.7

[0184] From the results in the above table, the impact of extremely high humidity on bean sprouts is slightly smaller than that of extremely high temperature, which may also be related to the fact that bean sprouts are a kind of plants that like humidity and have a very high tolerance to high humidity. However, as can be seen from the above characterization data, the impact of the first range interval on plants is still higher than that of the second range interval, which verifies the effectiveness of the interval selection criteria and the effectiveness of the light quality and light intensity range set by the present application for different temperature and humidity conditions.

[0185] Example 4

[0186] Based on the above research results, the company also carried out multi-crop tests in many provinces and regions of Jilin Province, Zhejiang Province, Hainan Province, Jiangxi Province and other provinces. In the multi-crop test, the growth trend improvement (Gt value, full score 100) and the dry weight relative nutritional value degree (Dv) were evaluated. The evaluation standard of the dry weight relative nutritional value degree was the same as the aforementioned standard, and the evaluation standard of the growth trend improvement was based on whether the crop was harvested or not, and the yield or the number of results was selected for evaluation. And the artificial greenhouse was used to construct the microclimate environment, and the greenhouse was an open type greenhouse.

[0187] The Gt value calculation is also based on the same plant as the control group under normal cultivation and development, and the same microclimate conditions but without laser light supplementing treatment as the comparison group. Gt value = (yield or number of results of the experimental group / yield or number of results of the control group) * 100. In order to save space, only some typical crop experimental results are displayed, and for the convenience of data observation and display:

[0188] When the Dv value is less than 80, it is represented by ×, when the Dv value reaches 80 or more, it is represented by the symbol When the Dv value reaches 85 or more, it is represented by the symbol ○, and when the DV value reaches 88 or more, it is represented by the symbol

[0189] When the Gt value is less than 85, it is represented by ×, when the Gt value reaches 90 or more, it is represented by the symbol When the Gt value reaches 95 or more, it is represented by the symbol ○, and when the Gt value reaches 98 or more, it is represented by the symbol

[0190] The experimental results are shown in the following table.

[0191]

[0192]

[0193] In the table, the Dv value evaluation standard of cotton is not the dry matter content of protein, carbohydrate and FFA, but the fiber length, fiber fineness and fiber strength are replaced, and the calculation method is similar.

[0194] From the above experimental results, it can be seen that the technical scheme of the present application can be applied to most plants, and can produce relatively significant growth regulation effect.

Claims

1. A method for regulating plant growth trend by laser, characterized in that, the method comprises: 1) forming a uniform laser light field by distributing laser light sources in an array; in the laser light sources: the central wavelength of red laser is 645-665 nm; the central wavelength of blue laser is 435-455 nm; the central wavelength of green laser is 535-565 nm; 2) adjusting the light quality and intensity of the laser light field according to the influencing factors of the microclimate environment of the planting site; the influencing factors of the microclimate environment include environmental temperature, relative humidity and ambient light intensity; the influencing factors of the microclimate environment are respectively determined as non-influencing factors, secondary influencing factors or main influencing factors according to the needs of the planted plants and the detected values; after the determination of the three influencing factors of the microclimate environment, when adjusting the laser light field, first, according to the main influencing factors, select the appropriate laser light quality and laser intensity range as the first range, and then according to the secondary influencing factors, select the appropriate laser light quality and laser intensity as the second range; when there is only one first range and at least one second range: if there is an overlapping interval between the first range and the second range, set the laser light quality and laser intensity according to the overlapping interval, if there is no overlapping interval, take the end value closest to the second range in the first range; when there are two first ranges: take the overlapping interval of the two first ranges and set the laser light quality and laser intensity according to the overlapping interval, if there is no overlapping interval, take the end value of any one of the two first ranges closest to the other first range, if there is a second range at this time, take the end value of the first range closest to the other first range with the highest overlapping degree or the second range; when there is no first range and there are multiple second ranges: take the overlapping interval of several second ranges and set the laser light quality and laser intensity according to the overlapping interval, if there is no overlapping interval, take the end value of any one of the several second ranges closest to the other second ranges.

2. The method for regulating plant growth trend by laser according to claim 1, characterized in that, 3. The method for regulating plant growth trend by laser according to claim 2, characterized in that, when setting the laser light field according to the environmental conditions, the light quality is taken as the main control parameter when the first first range is preferentially met, the end value intensity of the first first range is taken when the light quality intensity ratio parameter meets the other first range and / or the second range, and the green laser is adjusted; the green laser and the red laser are converted according to the intensity ratio of 1:0.80; the green laser and the blue laser are converted according to the intensity ratio of 1:1.

18. ​ ​ ​ ​ ​ ​ ​ At the current environmental temperature T am In the case that the plant can remain alive, the environmental temperature T am , the minimum temperature T min , the minimum temperature T sui-min , the maximum temperature T sui-max , and the maximum temperature T max are determined as follows: When T sui-min When T am When T sui-max the environmental temperature at this time is determined to be a non-influencing factor. When T min <T am <T sui-min or T sui-max <T am <T max the ambient temperature is determined to be a minor influencing factor; When T am <T min or T max <T am , the ambient temperature is determined as the main influencing factor. Current relative humidity RH am In case the lower plant can remain alive, the relative humidity RH am , the minimum humidity RH min , the minimum humidity RH sui-min , the maximum humidity RH sui-max , the maximum humidity RH max judgment: When RH sui-min < RH am < RH sui-max is determined that the relative humidity at this time is a non-influencing factor; When RH min <RH am <RH sui-min or RH sui-max <RH am <RH max At that time, relative humidity was determined to be a secondary influencing factor; When RH am <RH min or RH max <RH am At that time, relative humidity was determined to be the main influencing factor; Current ambient light intensity L am is a non-influencing factor or a minor influencing factor when the ambient light intensity L am is a non-influencing factor when the ambient light intensity L am is a minor influencing factor when the ambient light intensity L ​ ​ ​ ​ ​ The ambient temperature satisfies T min <T am <T sui-min When the ambient temperature satisfies T 2 The red and blue laser light has a total light intensity of 0.5-3.5 μmol / (m 2 ·s), and the intensity ratio of the red laser light to the blue laser light is (1.0-3.0):

1. The ambient temperature satisfies T sui-max <T am <T max When the ambient temperature satisfies T 2 0.5-2.0 μmol / (m 2 ·s), red and blue laser light with a light intensity ratio of 1:(1.0-3.0) is used for light supplementing treatment. The ambient temperature satisfies T am <T min When the ambient temperature satisfies T 2 The red and blue laser light has a total light intensity of 0.1-1.5 μmol / (m 2 ·s), and the intensity ratio of the red laser light to the blue laser light is (2.0-10.0):

1. The ambient temperature satisfies T max <T am When T 2 The red and blue laser light has a total light intensity of 0.1-0.5 μmol / (m 2 ·s), and the intensity ratio of the red laser light to the blue laser light is 1:(3.0-5.0). The relative humidity satisfies RH min < RH am < RH sui-min When the relative humidity satisfies RH 2 0.5-3.0 μmol / (m ·s) of red and blue laser light, wherein the intensity ratio of red laser light to blue laser light is (2.0-5.0):

1. The relative humidity satisfies RH sui-max < RH am < RH max When the relative humidity satisfies RH 2 The red and blue laser light has a total light intensity of 2.0-5.0 μmol / (m ·s), and the intensity ratio of the red laser light to the blue laser light is 1:(1.0-3.0). The relative humidity satisfies RH am <RH min When the relative humidity satisfies RH 2 The red and blue laser light has a total light intensity of 0.1-1.0 μmol / (m 2 ·s), and the intensity ratio of the red laser light to the blue laser light is (3.0-10.0):

1. The relative humidity satisfies RH max <RH am When the relative humidity satisfies RH 2 The red and blue laser light has a total light intensity of 0.2-1.5 μmol / (m ·s), and the intensity ratio of the red laser light to the blue laser light is 1:(2.5-5.0). The ambient light intensity L am ≤L-PPFD, the total light intensity is 0.5-5.0 μmol / (m 2 ·s) laser light is used for light supplementing treatment, wherein the intensity ratio of red laser light and blue laser light is 1: (0.1-5.0). ​ ​ ​ ​

Citation Information

Patent Citations

  • Artificial light supplementing method, system and equipment for plant cultivation

    CN115067097A

  • Plant factory operating system of mixed light type

    CN207284620U

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