An agricultural composition containing phyllotretol and brassinolide

Abstract

The application discloses an agricultural composition containing hemipholine and brassinosteroid, and belongs to the technical field of agricultural plant growth regulators. The effective component of the agricultural composition provided by the application is composed of hemipholine and brassinosteroid, and the mass ratio of the hemipholine to the brassinosteroid is 2.5-5.0:0.1-1.5. After the hemipholine and the brassinosteroid are compounded and applied to corn, the quality and yield of the corn are improved, and the agricultural composition has a synergistic effect on the yield improvement of the corn within the ratio range provided by the application. After the hemipholine and the brassinosteroid are compounded and applied to wheat, the yield of the wheat can be improved and the synergistic effect is also achieved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of plant growth regulators, and relates to an agricultural composition containing thidiazuron and brassinolide. BACKGROUND

[0002] Thidiazuron, also known as N-acetylthioproline, is a plant growth regulator, which mainly acts on the cell division and growth process of plant growth, promotes the synthesis and accumulation of substances such as protein, nucleic acid and alkaloid in the plant body, and also affects the hormone balance in the plant body, improves the adaptability of the plant to the environment, and enhances the drought resistance, cold resistance and disease resistance of the plant. Thidiazuron can regulate the osmotic pressure of plant cells, maintain the balance of water and nutrient transportation, promote seed germination and plant cell division and growth, keep chlorophyll from being lost, and improve the fruit setting rate and fruit yield.

[0003] Brassinolide is a plant growth regulator, which widely exists in the plant body, and can promote plant nutrient absorption in various stages of plant growth and development. The main effects include improving enzyme activity, improving seed vigor, promoting early development; enhancing photosynthetic efficiency, improving crop yield and quality; improving the cold resistance, drought resistance, waterlogging resistance and salt-tolerant ability of crops, and improving the stress resistance of crops; reducing pesticide damage and chemical pollution; resisting the damage of multiple viruses, bacteria and fungi, and enhancing the disease resistance of crops.

[0004] Since thidiazuron and brassinolide have different action directions, there is no report on the combination of thidiazuron and brassinolide for regulating crop growth in crop production practice. Therefore, it is of great significance to develop an agricultural composition containing thidiazuron and brassinolide as effective components, which can promote the growth and development of crops and improve the yield and quality of crops. SUMMARY

[0005] In order to develop an agricultural composition containing thidiazuron and brassinolide as effective components, which can promote the growth and development of corn and improve the yield and quality of corn, the present application provides an agricultural composition containing thidiazuron and brassinolide. The mass ratio of thidiazuron and brassinolide in the agricultural composition is 2.5-5.0:0.1-1.5. Within the ratio range provided by the present application, the agricultural composition has the effects of improving the quality and increasing the yield of crops, and the agricultural composition provided by the present application also has a synergistic effect on the yield improvement of crops.

[0006] To achieve the technical purposes of the present application, in one aspect, the present application provides an agricultural composition, the effective component of which is composed of semileaf and brassinolide. The mass ratio of semileaf and brassinolide in the agricultural composition is 2.5-5.0:0.1-1.5; preferably, the mass ratio of semileaf and brassinolide in the agricultural composition is 3.0-4.5:0.1-1.1; further preferably, the mass ratio of semileaf and brassinolide in the agricultural composition is 4.0:0.7.

[0007] In another aspect, the present application claims the use of the above-mentioned agricultural composition in improving the quality of corn, which includes improving the plant height, ear grain number and hundred-grain weight of corn.

[0008] Further, after the agricultural composition is applied to corn, it is found that, after semileaf and brassinolide are compounded at a mass ratio of 2.5-5.0:0.1-1.5, the plant height, ear grain number and hundred-grain weight of corn are all improved well, the plant height of corn is increased by 9.49-13.02%, the ear grain number of corn is increased by 7.19-16.50%, and the hundred-grain weight of corn is increased by 1.44-2.30%.

[0009] In addition, the present application claims the use of the above-mentioned agricultural composition in increasing the yield of crops, which are corn and / or wheat.

[0010] Further, after the agricultural composition is applied to corn, it is found that the agricultural composition containing semileaf and brassinolide of the present application has the effect of increasing the yield of corn, and also has a synergistic effect within the ratio range provided in the present application. Semileaf and brassinolide compounded at a mass ratio of 2.5-5.0:0.1-1.5 have the effect of increasing the yield of corn, and the yield increase rate is 8.83-19.66%; when the mass ratio of semileaf and brassinolide is 3.0-4.5:0.1-1.1, the agricultural composition has a better effect of increasing the yield of corn, and the yield increase rate is 15.92-19.66%, and the difference between the actual yield increase rate and the theoretical yield increase rate is greater than 10%, showing a synergistic effect; when the mass ratio of semileaf and brassinolide is 4.0:0.7, the synergistic effect is best, and the difference between the actual yield increase rate and the theoretical yield increase rate is 11.73%.

[0011] Further, the application found that the agricultural composition containing semileaf and brassinosteroid has the effect of increasing the yield of wheat after the application of the agricultural composition to wheat, and has a synergistic effect within the ratio range provided in the application. The semileaf and brassinosteroid have the effect of increasing the yield of wheat within the ratio range of 2.5-5.0:0.1-1.5, and the yield of the agricultural composition to wheat is 9.33-14.58%; when the ratio of semileaf and brassinosteroid is 3.0-4.5:0.1-1.1, the yield of the agricultural composition to wheat is better, the yield is 13.21-14.58%, and the difference between the actual yield and the theoretical yield is greater than 10%, showing a synergistic effect; when the ratio of semileaf and brassinosteroid is 4.0:0.7, the synergistic effect is best, and the difference between the actual yield and the theoretical yield is 10.77%.

[0012] Compared with the prior art, the technical scheme provided by the application has at least the following beneficial effects or advantages:

[0013] The agricultural composition containing semileaf and brassinosteroid of the application has the effect of improving the quality of corn. After the semileaf and brassinosteroid are compounded in a mass ratio of 2.5-5.0:0.1-1.5, the plant height, ear grain number and hundred-grain weight of corn are all improved, the plant height of corn is increased by 9.49-13.02%, the ear grain number of corn is increased by 7.19-16.50%, and the hundred-grain weight of corn is increased by 1.44-2.30%. The application of semileaf or brassinosteroid alone also has the effect of promoting the improvement of corn quality, but the promoting effect is poorer than that of the agricultural composition compounded by the application; when the ratio exceeds the ratio range provided in the application, the effect of the agricultural composition on improving the quality of corn is poor; when the semileaf is replaced by gibberellin or the brassinosteroid is replaced by ethylene, the effect of the agricultural composition on improving the quality of corn is reduced.

[0014] The agricultural composition containing semileaf and brassinosteroid of the application has the effect of increasing the yield of corn, and has a synergistic effect within the ratio range provided in the application. The semileaf and brassinosteroid have the effect of increasing the yield of corn within the mass ratio range of 2.5-5.0:0.1-1.5, and the yield is 8.83-19.66%; when the mass ratio of semileaf and brassinosteroid is 3.0-4.5:0.1-1.1, the yield of the agricultural composition to corn is better, the yield is 15.92-19.66%, and the difference between the actual yield and the theoretical yield is greater than 10%, showing a synergistic effect; when the mass ratio of semileaf and brassinosteroid is 4.0:0.7, the synergistic effect is best, and the difference between the actual yield and the theoretical yield is 11.73%. When the ratio exceeds the ratio range provided in the application or the components of the agricultural composition of the application are changed, the yield of corn shows an additive effect, which is poorer than that of the agricultural composition of the application.

[0015] The agricultural composition containing hemipholine and brassinolide of the present application has the effect of increasing the yield of wheat, and has a synergistic effect within the ratio range provided in the present application. The hemipholine and brassinolide have the effect of increasing the yield of wheat within the ratio range of 2.5-5.0:0.1-1.5, and the yield-increasing rate of the agricultural composition on wheat is 9.33-14.58%; when the ratio of hemipholine and brassinolide is 3.0-4.5:0.1-1.1, the yield-increasing effect of the agricultural composition on wheat is better, the yield-increasing rate reaches 13.21-14.58%, and the difference between the actual yield-increasing rate and the theoretical yield-increasing rate is greater than 10%, showing a synergistic effect; when the ratio of hemipholine and brassinolide is 4.0:0.7, the synergistic effect is best, and the difference between the actual yield-increasing rate and the theoretical yield-increasing rate is 10.77%. DETAILED DESCRIPTION

[0016] Hereinafter, the technical solutions of the present application will be described in combination with examples, but the present application is not limited to the following examples. The experimental methods and detection methods described in each example are all conventional methods, unless otherwise specified; and the reagents and materials described, unless otherwise specified, can be purchased on the market.

[0017] Hemipholine was purchased from Shandong Lianfan Chemical Co., Ltd., with a purity of 99%.

[0018] Brassinolide was purchased from Shanghai Jizhishe Biochemical Technology Co., Ltd., with a purity of 95%.

[0019] Gibberellin was purchased from Beijing Yita Biological Technology Co., Ltd., with a purity of 98%.

[0020] Ethrel was purchased from Shanghai Jizhishe Biochemical Technology Co., Ltd., with a purity of 90%.

[0021] Example 1

[0022] The present example provides the preparation dosage form of the agricultural composition containing hemipholine and brassinolide.

[0023] 1. Emulsifiable concentrate

[0024] Each 100 kg of the emulsifiable concentrate containing hemipholine and brassinolide includes 2.53 kg of hemipholine, 0.11 kg of brassinolide, 30 kg of N-methylpyrrolidone, 2 kg of sodium dodecylbenzenesulfonate, 0.5 kg of silicone, 10 kg of ethyl acetate, and soybean oil to make up to 100 kg.

[0025] Each component is weighed according to the above ratio, and the semileaf and brassinolide are added into N-methylpyrrolidone and mixed uniformly, and then sodium dodecyl benzene sulfonate, silicone, ethyl acetate, and soybean oil are sequentially added and stirred uniformly to form a uniform transparent oily liquid, thereby obtaining the semileaf and brassinolide-containing emulsifiable concentrate.

[0026] 2. Wettable powder

[0027] Each 100 kg of the semileaf and brassinolide-containing wettable powder comprises 3.03 kg of semileaf, 0.32 kg of brassinolide, 5 kg of white carbon black, 2 kg of dextrin, 5 kg of lignin sulfonate, 3 kg of carboxymethyl cellulose, 5 kg of sodium dodecyl sulfonate, and kaolin to 100 kg.

[0028] Each component is weighed according to the above ratio, and the semileaf and brassinolide are added into N-methylpyrrolidone and mixed uniformly, and then sodium dodecyl benzene sulfonate, silicone, ethyl acetate, and soybean oil are sequentially added and stirred uniformly to form a uniform transparent oily liquid, thereby obtaining the semileaf and brassinolide-containing emulsifiable concentrate.

[0029] 3. Water dispersible granule

[0030] Each 100 kg of the semileaf and brassinolide-containing water dispersible granule comprises 4.04 kg of semileaf, 0.74 kg of brassinolide, 10 kg of lignin sulfonate, 5 kg of sodium dodecyl sulfonate, 5 kg of polyvinylpyrrolidone, 3 kg of sodium carboxymethyl cellulose, 2 kg of dextrin, and kaolin to 100 kg.

[0031] Each component is weighed according to the above ratio, and the semileaf and brassinolide are added into N-methylpyrrolidone and mixed uniformly, and then sodium dodecyl benzene sulfonate, silicone, ethyl acetate, and soybean oil are sequentially added and stirred uniformly to form a uniform transparent oily liquid, thereby obtaining the semileaf and brassinolide-containing emulsifiable concentrate.

[0032] Example 2

[0033] The present embodiment provides a field test of the effect of the semileaf and brassinolide-containing agricultural composition on corn.

[0034] The semileaf and brassinolide are compounded into the water dispersible granule in the manner of Example 1, and after being diluted by 30 times, a knapsack electric sprayer is used to perform foliar spraying treatment on corn in the three-leaf stage, with a spraying amount of 30 kg per mu, and the tested corn variety being Shaankoe No. 6.

[0035] The tested site is a corn planting base in Yijun County, Tongchuan City, Shaanxi Province, and a plot test method is used, with a test plot area of 24 m 2 , randomly arranged in groups, and repeated four times. In the middle and late April, seeds are sown with a row spacing of 40 cm and a plant spacing of 60 cm. During the test period, no other plant growth regulators are used, and field fertilization, water, and pest management are strictly in accordance with the requirements of corn planting management, and the test area cultivation management conditions are uniform and consistent, and meet the local scientific agricultural cultivation measures.

[0036] The experiment involved 26 treatments, respectively, test 1-7: only apply half leaf; test 8-14 only apply brassinolide; test 15: only apply gibberellin; test 16: only apply ethylene; test 17: the mass ratio of half leaf and brassinolide is 2.0:0.05; test 18: the mass ratio of half leaf and brassinolide is 2.5:0.1; test 19: the mass ratio of half leaf and brassinolide is 3.0:0.4; test 20: the mass ratio of half leaf and brassinolide is 4.0:0.7; test 21: the mass ratio of half leaf and brassinolide is 4.5:1.1; test 22: the mass ratio of half leaf and brassinolide is 5.0:1.5; test 23: the mass ratio of half leaf and brassinolide is 6.0:2.0; test 24: replace half leaf with gibberellin, and the effective components of gibberellin and brassinolide are added in the same amount as test 20; test 25: replace brassinolide with ethylene, and the effective components of half leaf and ethylene are added in the same amount as test 20; test 26: apply the same amount of water, which is the blank control group. Random five-point sampling method is used in each plot, and 4 corns are investigated at each point. The plant height, ear row number, ear grain number and hundred-grain weight of corn are recorded, and the results are shown in Table 1; the total yield of corn in different test groups is counted, and the results are shown in Table 2, and the yield increase rate in Table 2 is calculated as follows:

[0037] Actual yield increase rate E(%) = [(yield of pesticide treatment group-yield of blank control group) / yield of blank control group] x 100

[0038] Theoretical yield increase rate E0(%) of two pesticide compound application = X+Y-XY / 100

[0039] In the formula, X represents the actual yield increase rate of half leaf when the dosage is P; Y represents the actual yield increase rate of brassinolide when the dosage is Q; the combined effect type of the mixture is evaluated according to Gowing method in NY / T2061.5-2016, E-E0>10% is synergistic effect, E-E0<-10% is antagonistic effect, and the value of E-E0 is within ±10% is additive effect.

[0040] Table 1: Effect of agricultural composition containing half leaf (A) and brassinolide (B) on corn

[0041]

[0042] As shown in Table 1, after semileaf and brassinolide are compounded according to the mass ratio of 2.5-5.0:0.1-1.5, the plant height, ear grain number and hundred-grain weight of corn are all improved, the plant height of corn is increased by 9.49-13.02%, the ear grain number of corn is increased by 7.19-16.50%, and the hundred-grain weight of corn is increased by 1.44-2.30%, which indicates that the application of the agricultural composition of the application can improve the quality of corn. The application of semileaf or brassinolide alone can also promote the improvement of corn quality, but the promoting effect is poorer than that of the agricultural composition after compounding according to the application, and the promoting effect of the agricultural composition on the quality of corn is poor when the ratio is beyond the range provided by the application; the effect of the agricultural composition on the quality of corn is reduced when semileaf is replaced by gibberellin or brassinolide is replaced by ethephon.

[0043] Table 2: Effect of the agricultural composition containing semileaf (A) and brassinolide (B) on corn

[0044]

[0045]

[0046] As shown in Table 2, the agricultural composition has a yield-increasing effect on corn when semileaf and brassinolide are compounded within the ratio range of 2.5-5.0:0.1-1.5, and the yield-increasing rate of the agricultural composition on corn is 8.83-19.66%; the yield-increasing effect of the agricultural composition on corn is better when the ratio of semileaf and brassinolide is 3.0-4.5:0.1-1.1, and the yield-increasing rate reaches 15.92-19.66%, and the difference between the actual yield-increasing rate and the theoretical yield-increasing rate is greater than 10%, which shows a synergistic effect; the synergistic effect is best when the ratio of semileaf and brassinolide is 4.0:0.7, and the difference between the actual yield-increasing rate and the theoretical yield-increasing rate is 11.73%. The yield-increasing rate on corn shows an additive effect when the ratio is beyond the range provided by the application or the components of the agricultural composition of the application are changed, and the effect is poorer than that of the agricultural composition of the application.

[0047] Example 3

[0048] The present embodiment provides a field test of the effect of the agricultural composition containing semileaf and brassinolide on corn.

[0049] Semileaf and brassinolide are compounded into emulsifiable concentrate according to the mode of Example 1, and after being diluted by 30 times, a knapsack electric sprayer is used to spray the leaves of wheat at the jointing stage, and the spraying amount is 30 kg / acre, and the tested wheat variety is Xinong 511.

[0050] The test site is a wheat planting base in Linyou County, Baoji City, Shaanxi Province, and a plot test method is used, and the test plot area is 20 m 2, randomized block design, four times of repetition. The seeding was in early October, the plot length was 5 m, 10 rows of area, the row spacing was 20 cm, the ridge was evenly set between plots and blocks, 1 m protection row was set around the test field. No other plant growth regulator was used during the test, the field fertilization and water and pest management were strictly in accordance with the requirements of wheat planting management, and the test area cultivation management conditions were uniform and consistent, and met the local scientific agricultural cultivation measures.

[0051] The test involved 26 treatments, which were test 27-31: only hemerocallus; test 32-36: only brassinolide; test 37: the mass ratio of hemerocallus and brassinolide was 2.5:0.1; test 38: the mass ratio of hemerocallus and brassinolide was 3.0:0.4; test 39: the mass ratio of hemerocallus and brassinolide was 4.0:0.7; test 40: the mass ratio of hemerocallus and brassinolide was 4.5:1.1; test 41: the mass ratio of hemerocallus and brassinolide was 5.0:1.5; test 42: the same amount of water was applied, which was a blank control group. The total yield of wheat in different test groups was counted, and the statistical results were shown in Table 3, and the yield increase rate calculation formula in Table 3 was the same as that in Example 2.

[0052] Table 3: Effect of agricultural composition containing hemerocallus (A) and brassinolide (B) on wheat

[0053]

[0054]

[0055] It can be known from Table 3 that hemerocallus and brassinolide had yield-increasing effect on wheat after compounding in the ratio range of 2.5-5.0:0.1-1.5, and the yield-increasing rate of the agricultural composition on wheat was 9.33-14.58%; when the ratio range of hemerocallus and brassinolide was 3.0-4.5:0.1-1.1, the yield-increasing effect of the agricultural composition on wheat was better, the yield-increasing rate reached 13.21-14.58%, and the difference between the actual yield-increasing rate and the theoretical yield-increasing rate was greater than 10%, which showed synergistic effect; when the ratio of hemerocallus and brassinolide was 4.0:0.7, the synergistic effect was best, and the difference between the actual yield-increasing rate and the theoretical yield-increasing rate was 10.77%.

[0056] The above-described examples are part of the embodiments of the present application, rather than all the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but only represents selected embodiments of the present application. All other embodiments obtained by related deduction and replacement of those skilled in the art under the condition of the concept of the present application, without creative labor, belong to the scope of protection of the present application.

Claims

1. An agricultural composition characterized in that, The effective component of the agricultural composition consists of hemipholine and brassinolide, and the mass ratio of hemipholine to brassinolide is 2.5-5.0:0.1-1.

5.

2. The agricultural composition of claim 1, wherein, The mass ratio of hemipholine to brassinolide in the agricultural composition is 3.0-4.5:0.1-1.

1.

3. The agricultural composition of claim 1, wherein, The mass ratio of hemipholine to brassinolide in the agricultural composition is 4.0:0.

7.

4. Use of the agricultural composition according to any one of claims 1 to 3 for improving the quality of corn, characterized in that, The improvement of the quality of corn includes the increase of plant height, ear grain number and hundred-grain weight.

5. Use of the agricultural composition according to any one of claims 1-3 in increasing the yield of crops.

6. Use according to claim 5, characterized in that, The crops are corn and / or wheat.

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