A plant complex enriched in gamma-aminobutyric acid and a method for its preparation and use

Abstract

The present application belongs to the technical field of food processing, and specifically provides a plant compound rich in gamma-aminobutyric acid, which comprises plant raw materials and a double-spore mushroom enzyme treatment liquid; the double-spore mushroom enzyme treatment liquid comprises double-spore mushrooms, cellulase, yeast and acetic acid bacteria. The plant compound provided by the present application is prepared from coarse grain raw materials and double-spore mushrooms after corresponding process treatment, stimulates the accumulation of GABA in the germination process of coarse grains, greatly improves the content of plant-based natural GABA, has simple process, is economical and safe, and is suitable for industrial production. The present application also provides a plant fermentation liquid rich in gamma-aminobutyric acid and soluble polysaccharides and a sleep-promoting fermented beverage based on the plant compound, which enriches natural GABA and mushroom polysaccharides while reducing the large loss of GABA in the beverage obtained by natural plant-based fermentation caused by conventional processes. Not only can the human body immunity be improved, but also the production of sleep-related neurotransmitters secreted naturally by the human body can be promoted, thereby jointly promoting sleep from the inside to the outside.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of food processing, and particularly relates to a plant compound rich in gamma-aminobutyric acid and a preparation method and application thereof. BACKGROUND

[0002] Gamma-aminobutyric acid (GABA) is a four-carbon non-protein amino acid widely existing in microorganisms, plants and vertebrates. In mammals, GABA mainly acts as an inhibitory neurotransmitter to play an important physiological function, has a relieving effect on symptoms such as insomnia, depression, anxiety and Alzheimer's disease, and can improve the memory function of the brain. In addition, GABA also has the functions of reducing blood lipids, preventing obesity and maintaining the health of the gastrointestinal system. With the increase of age and mental stress, the content of GABA in the human body gradually decreases. Recent studies have shown that exogenous GABA can be ingested through oral administration.

[0003] The synthesis methods of GABA mainly include chemical synthesis, microbial fermentation and plant metabolism. Plant metabolism refers to a way in which tea, grains, beans and the like accumulate GABA under germination, mechanical damage and other conditions. The plant metabolism method is more natural and green compared with chemical synthesis and microbial fermentation, and meets the requirements of safety and appropriate amount of GABA. There are also attempts to use grains as raw materials to gradually accumulate effective components by combining the above physical or chemical methods with microbial fermentation technology, in order to solve the problems of taste and product efficacy.

[0004] According to research, the natural production of GABA is greatly affected by high temperature (≥80℃), pH (≥pH6 or ≤pH4.5) and time. In the production process of plant-based enzyme, pretreatment by enzyme before fermentation can quickly decompose the nutritional components in grains for the use of microorganisms, which is efficient, economical and convenient. However, the high temperature and pH changes in the processes of enzyme treatment, sterilization and fermentation will cause different degrees of loss of GABA, ultimately resulting in too little or even almost no GABA in the beverage obtained by natural plant-based fermentation. Therefore, additional GABA needs to be added to make up for the loss caused by the process. The additional GABA may cause safety problems in raw material sources due to cost, and cannot guarantee the quality of the product.

[0005] Quinoa not only contains nutrients such as starch, protein, unsaturated fatty acids, vitamins and minerals, but also contains rich active ingredients such as polyphenols, flavonoids, saponins and gamma-aminobutyric acid. Double-spore mushroom is a kind of health food containing high protein, low fat and low heat. Mushroom polysaccharides and isoprotein in double-spore mushroom have anticancer effect; double-spore mushroom has antioxidant, anti-inflammatory and antitumor effects. However, there are few high-value-added products, and the deep processing level is far behind. SUMMARY

[0006] The present application aims to overcome the problems of large loss in the natural GABA extraction process, and lack of deep processing technology for GABA-rich plant raw materials and Agaricus bisporus.

[0007] To this end, the present application provides a plant complex rich in gamma-aminobutyric acid, comprising plant raw materials and an Agaricus bisporus enzyme treatment liquid; the Agaricus bisporus enzyme treatment liquid comprises Agaricus bisporus, cellulase, yeast and acetic acid bacteria.

[0008] Specifically, the mass ratio of the plant raw materials to the Agaricus bisporus enzyme treatment liquid in the plant complex is (2-3):1.

[0009] Specifically, the Agaricus bisporus enzyme treatment liquid comprises Agaricus bisporus, cellulase, yeast and acetic acid bacteria at a mass ratio of 100:(1.5-3.2):(1.8-3.2):(0.6-2.0).

[0010] The present application also provides a preparation method of the plant complex rich in gamma-aminobutyric acid, comprising the following steps:

[0011] (1) Agaricus bisporus is crushed and mixed with water to obtain Agaricus bisporus slurry, cellulase is added to the Agaricus bisporus slurry, and yeast and acetic acid bacteria are inoculated for fermentation to obtain an Agaricus bisporus enzyme treatment liquid;

[0012] (2) the plant raw materials are mixed with the Agaricus bisporus enzyme treatment liquid for germination, and the obtained mixture is dried after germination to obtain the plant complex rich in gamma-aminobutyric acid.

[0013] Specifically, the fermentation temperature in step (1) is 30-32℃, and the fermentation time is 24-30h.

[0014] Specifically, the germination temperature in step (2) is 25-30℃, and the germination time is 48-72h.

[0015] The present application also provides a plant fermentation liquid rich in gamma-aminobutyric acid and soluble polysaccharides, comprising a plant complex, amylase, papain, Lactobacillus rhamnosus and Lactobacillus plantarum; the plant complex is the plant complex rich in gamma-aminobutyric acid.

[0016] The present application also provides a preparation method of the plant fermentation liquid rich in gamma-aminobutyric acid and soluble polysaccharides, comprising the following steps:

[0017] (1) the plant complex is mixed with water to obtain a composite powder slurry, the pH of the composite powder slurry is adjusted, amylase and papain are added for enzymolysis to obtain a primary enzymolysis liquid;

[0018] (2) Inoculate Lactobacillus rhamnosus and Lactobacillus plantarum into the primary enzymatic hydrolysate for fermentation, centrifuge to remove the filter residue, and obtain a plant fermentation broth rich in γ-aminobutyric acid and soluble polysaccharides.

[0019] Specifically, in step (1) above, after adding amylase to the composite slurry for 20-30 minutes of enzymatic hydrolysis, add papain for 15-30 minutes of enzymatic hydrolysis to obtain the primary hydrolysate.

[0020] The present invention also provides a sleep-promoting fermented beverage, comprising plant fermentation liquid, jujube seed and lily; wherein the plant fermentation liquid is the above-mentioned plant fermentation liquid rich in γ-aminobutyric acid and soluble polysaccharides.

[0021] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0022] The plant complex rich in γ-aminobutyric acid (GABA) provided by this invention is prepared from plant raw materials and button mushrooms through appropriate processing. The process is simple, economical, safe, and suitable for industrial production. Button mushrooms are rich in protein, amino acids, and polysaccharides. Through the synergistic fermentation of cellulase, yeast, and acetic acid bacteria, cellulose is efficiently degraded, releasing more soluble polysaccharides and small molecule nutrients such as glutamic acid and arginine, while simultaneously lowering the environmental pH to 4.5-5.5. Quinoa is then germinated in a button mushroom enzyme treatment solution. The pH of the treatment solution (4.5-5.5) reduces the chance of contamination by other microorganisms during quinoa germination, significantly extending the germination time and facilitating the accumulation of more GABA. Simultaneously, the small molecule amino acids such as glutamic acid and arginine accumulated in the button mushroom enzyme treatment solution further stimulate GABA accumulation during quinoa germination, greatly increasing the content of plant-based natural GABA.

[0023] This invention provides a plant fermentation broth rich in γ-aminobutyric acid (GABA) and soluble polysaccharides. Using a plant complex rich in GABA as a substrate, it employs a dual-enzyme approach for short-term enzymatic hydrolysis. This initial enzymatic hydrolysis facilitates microbial utilization and simultaneously acts as a bactericide, minimizing GABA loss due to the carbonyl-amine reaction. While enriching natural GABA and mushroom polysaccharides, it reduces the significant GABA loss in beverages obtained from natural plant-based fermentation caused by conventional processes. The polysaccharides, dietary fiber, and organic acids obtained from this process promote the growth of beneficial intestinal bacteria, thus promoting intestinal health.

[0024] The sleep-promoting fermented beverage provided by this invention combines plant fermentation liquid rich in γ-aminobutyric acid and soluble polysaccharides with jujube seed and lily bulb. It has excellent flavor and outstanding effects. It can not only improve human immunity, but also promote the production of sleep-related neurotransmitters (GABA, 5-hydroxytryptamine, melatonin) naturally secreted by the human body, thus promoting sleep from the inside out. Detailed Implementation

[0025] The technical solutions of the present invention will be clearly and completely described below with reference to embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Although representative embodiments of the present invention have been described in detail, those skilled in the art will understand that various modifications and changes can be made to the present invention without departing from the scope of the present invention. Therefore, the scope of the present invention should not be limited to the embodiments, but should be defined by the appended claims and their equivalents.

[0026] This invention provides a plant complex rich in γ-aminobutyric acid (GABA), comprising plant raw materials and a *Agaricus bisporus* enzyme treatment solution; the *Agaricus bisporus* enzyme treatment solution comprises *Agaricus bisporus*, cellulase, yeast, and acetic acid bacteria. The plant raw materials preferably include whole grains such as quinoa and brown rice; the quinoa is whole quinoa, and can be selected from any one of white quinoa, red quinoa, or black quinoa.

[0027] The mass ratio of plant raw materials to button mushroom enzyme treatment solution is (2-3):1; the button mushroom enzyme treatment solution includes button mushroom, cellulase, yeast and acetic acid bacteria in a mass ratio of 100:(1.5-3.2):(1.8-3.2):(0.6-2.0).

[0028] The present invention also provides a method for preparing the above-mentioned plant complex, comprising the following steps:

[0029] (1) Select high-quality, intact button mushrooms with cap stems, clean them, blanch them in boiling water, dry them, and grind them into button mushroom powder for later use.

[0030] After sterilizing the button mushroom powder with ultraviolet light, it is mixed with water at a ratio of 1:(2-3) to obtain button mushroom slurry. Cellulase is added to the button mushroom slurry and yeast and acetic acid bacteria are inoculated for fermentation to obtain button mushroom enzyme treatment solution.

[0031] During fermentation, the pH should be controlled at 4.5-5.5, and fermentation should be carried out at 30-32℃ for 24-30 hours.

[0032] The pH of the treatment solution (4.5-5.5) can reduce the chance of contamination by other microorganisms during the subsequent quinoa germination process, greatly prolong the germination time of quinoa, and facilitate the accumulation of more GABA.

[0033] (2) Select high-quality plant materials, clean and remove ash, mix with Agaricus bisporus enzyme treatment solution, germinate at 25-30℃ for 48-72h to complete the natural accumulation of GABA. After germination, dry the resulting mixture to obtain a plant complex rich in γ-aminobutyric acid.

[0034] The present invention also provides a plant fermentation broth rich in γ-aminobutyric acid and soluble polysaccharides, comprising a plant complex, amylase, papain, Lactobacillus rhamnosus, and Lactobacillus plantarum; wherein the plant complex is the above-mentioned plant complex rich in γ-aminobutyric acid.

[0035] The present invention also provides a method for preparing the above-mentioned plant fermentation broth, comprising the following steps:

[0036] (1) Mix the plant compound with water to form a compound slurry according to a material-to-liquid ratio of 1:(3-5), adjust the pH of the compound slurry to 4.5-5, add amylase and papain to it in sequence for enzymatic hydrolysis, and obtain primary hydrolysate.

[0037] Based on the weight of the composite slurry, the preferred amount of amylase added is 1-2%, and the preferred amount of papain added is 2-3%.

[0038] Optionally, after adjusting the pH, heat to 50-60℃, add amylase for 20-30 minutes of enzymatic hydrolysis, and then add papain for 15-30 minutes of enzymatic hydrolysis to obtain the primary enzymatic hydrolysate.

[0039] Short-term enzymatic hydrolysis at 50℃ using two enzymes serves two purposes: firstly, it facilitates microbial utilization after preliminary hydrolysis, and secondly, it has a bactericidal effect, minimizing the loss of GABA caused by the carbonyl-amine reaction.

[0040] (2) Inoculate Lactobacillus rhamnosus and Lactobacillus plantarum into the primary enzymatic hydrolysate, preferably ferment at 35-37℃ for 15-20h to complete the accumulation of GABA, centrifuge to remove the filter residue, and obtain a plant fermentation broth rich in γ-aminobutyric acid and soluble polysaccharides.

[0041] Based on the mass of the primary enzymatic hydrolysate, the preferred inoculum size of *Lactobacillus rhamnosus* is 1.2-1.5%, and the preferred inoculum size of *Lactobacillus plantarum* is 0.4-0.8%.

[0042] This invention also provides a sleep-promoting fermented beverage, comprising a plant fermentation liquid, jujube seed, and lily bulb; the plant fermentation liquid is the aforementioned plant fermentation liquid rich in γ-aminobutyric acid and soluble polysaccharides. The preferred amount of jujube seed added, based on the mass of the plant fermentation liquid, is 0.5-1%, and the preferred amount of lily bulb added is 1-2%.

[0043] The following specific embodiments illustrate the effects of the plant complex rich in γ-aminobutyric acid of the present invention, its preparation method, and its application.

[0044] Example 1:

[0045] This embodiment provides a plant complex rich in γ-aminobutyric acid, which is prepared using the following steps.

[0046] (1) Pretreatment of button mushrooms: Select high-quality, intact button mushrooms with cap stems, clean them, blanch them in boiling water for 3 minutes, drain them, dry them at 50-60℃, and grind them into button mushroom powder for later use.

[0047] After irradiating button mushroom powder with ultraviolet light for 30 minutes, it is mixed with sterilized purified water at a mass ratio of 1:2 to obtain button mushroom slurry.

[0048] Pre-fermentation: Based on the mass of button mushroom slurry, add 0.5% acidic cellulase to the button mushroom slurry, and simultaneously inoculate with 0.6% yeast and 0.2% acetic acid bacteria, control the pH at 4.5, and ferment at 30℃ for 24 hours to obtain button mushroom enzyme treatment solution.

[0049] (2) Quinoa pretreatment: Select high-quality quinoa, wash and remove ash, mix with Agaricus bisporus enzyme treatment solution at a mass ratio of 2:1, germinate at 25℃ for 48h, and freeze-dry the resulting mixture to obtain plant complex.

[0050] The GABA content was found to be 345 mg / 100g.

[0051] Comparative Example 1:

[0052] This comparative example provides a plant-based compound prepared using the following steps.

[0053] (1) Pretreatment of button mushrooms: Select high-quality, intact button mushrooms with cap stems, clean them, blanch them in boiling water for 3 minutes, drain them, dry them at 50-60℃, and grind them into button mushroom powder for later use.

[0054] After irradiating button mushroom powder with ultraviolet light for 30 minutes, it is mixed with sterilized purified water at a mass ratio of 1:2 to obtain button mushroom slurry.

[0055] (2) Quinoa pretreatment: High-quality quinoa was selected, washed and cleaned, and then mixed with button mushroom slurry at a mass ratio of 2:1. The mixture was germinated at 25℃ for 48 hours, and the resulting mixture was freeze-dried to obtain a plant complex. The GABA content was found to be 126 mg / 100 g.

[0056] Comparative Example 2:

[0057] This comparative example provides quinoa flour prepared using the following steps: high-quality quinoa was selected, washed to remove ash, and germinated at 25°C for 48 hours. The resulting mixture was then freeze-dried to obtain quinoa flour. The GABA content was determined to be 76 mg / 100g.

[0058] Example 2:

[0059] This embodiment provides a plant fermentation broth based on the plant complex rich in γ-aminobutyric acid provided in Example 1, which is prepared using the following steps.

[0060] (1) Mix the plant compound with pure water at a mass ratio of 1:3 to form a compound slurry.

[0061] (2) Adjust the pH of the composite slurry to 4.5 with acetic acid and then heat it to 50°C. Based on the mass of the composite slurry, add 1% medium-temperature amylase for 20 min and 2% papain for 15 min to obtain the primary hydrolysate.

[0062] (3) Based on the mass of the primary enzyme hydrolysate, inoculate the primary enzyme hydrolysate slurry with 1.2% Lactobacillus rhamnosus and 0.4% Lactobacillus plantarum, and ferment at 35°C for 15 hours.

[0063] (4) After centrifugation to remove the filter residue, a plant fermentation broth rich in γ-aminobutyric acid and soluble polysaccharides is obtained.

[0064] This embodiment also provides a sleep-promoting fermented beverage, which is prepared by the following steps: 0.5% jujube seed powder and 1% lily powder are added to the above-mentioned plant fermentation liquid rich in γ-aminobutyric acid and soluble polysaccharides by weight. After mixing evenly, the mixture is sterilized at 100°C for 5 minutes to obtain the sleep-promoting fermented beverage. Its GABA content and polysaccharide yield are detected, and the results are shown in Table 1.

[0065] Comparative Example 3:

[0066] This comparative example, based on the plant complex of Comparative Example 1, provides a plant fermentation broth prepared using the following steps.

[0067] (1) Mix the plant compound with pure water at a mass ratio of 1:3 to form a compound slurry.

[0068] (2) Adjust the pH of the composite slurry to 4.5 with acetic acid and then heat it to 50°C. Based on the mass of the composite slurry, add 1% medium-temperature amylase for 20 min and 2% papain for 15 min to obtain the primary hydrolysate.

[0069] (3) Based on the mass of the primary enzyme hydrolysate, inoculate the primary enzyme hydrolysate slurry with 1.2% Lactobacillus rhamnosus and 0.4% Lactobacillus plantarum, and ferment at 35°C for 15 hours.

[0070] (4) The filter residue is removed by centrifugation to obtain plant fermentation liquid.

[0071] This comparative example also provides a fermented beverage prepared by the following steps: 0.5% jujube seed powder and 1% lily powder were added to the above-mentioned plant fermentation liquid by weight, and after being mixed evenly, the mixture was sterilized at 100°C for 5 minutes to obtain the fermented beverage. The GABA content and polysaccharide yield were tested, and the results are shown in Table 1.

[0072] Comparative Example 4:

[0073] This comparative example provides a plant fermentation broth based on the γ-aminobutyric acid-rich plant complex of Example 1, prepared using the following steps.

[0074] (1) Mix the plant compound with an appropriate amount of pure water at a mass ratio of 1:3 to form a compound slurry.

[0075] (2) Based on the mass of the composite slurry, inoculate the composite slurry with 1.2% Lactobacillus rhamnosus and 0.4% Lactobacillus plantarum, and ferment at 35°C for 15 hours.

[0076] (3) The filter residue was removed by centrifugation to obtain plant fermentation liquid.

[0077] This comparative example also provides a fermented beverage prepared by the following steps: 0.5% jujube seed powder and 1% lily powder were added to the above-mentioned plant fermentation liquid by weight, and after being mixed evenly, the mixture was sterilized at 100°C for 5 minutes to obtain the fermented beverage. The GABA content and polysaccharide yield were tested, and the results are shown in Table 1.

[0078] Comparative Example 5:

[0079] This comparative example, based on the quinoa powder of Comparative Example 2, provides a plant fermentation liquid prepared using the following steps.

[0080] (1) Pretreatment of button mushrooms: Select high-quality, intact button mushrooms with cap stems, clean them, blanch them in boiling water for 3 minutes, drain them, dry them at 50-60℃, and grind them into button mushroom powder for later use.

[0081] (2) Mix the button mushroom powder, quinoa powder and pure water in a mass ratio of 1:2:3 to form a composite slurry.

[0082] (2) Heat to 50℃, and add 1% medium temperature amylase for 20 min and 2% papain for 15 min by weight of composite slurry to obtain primary hydrolysate.

[0083] (3) Based on the mass of the primary enzyme hydrolysate, inoculate the primary enzyme hydrolysate slurry with 1.2% Lactobacillus rhamnosus and 0.4% Lactobacillus plantarum, and ferment at 35°C for 15 hours.

[0084] (4) The filter residue is removed by centrifugation to obtain plant fermentation liquid.

[0085] This comparative example also provides a fermented beverage prepared by the following steps: 0.5% jujube seed powder and 1% lily powder were added to the above-mentioned plant fermentation liquid by weight, and after being mixed evenly, the mixture was sterilized at 100°C for 5 minutes to obtain the fermented beverage. The GABA content and polysaccharide yield were tested, and the results are shown in Table 1.

[0086] Table 1. GABA content and polysaccharide yield in various fermented beverages

[0087]

[0088]

[0089] Example 3:

[0090] This embodiment uses the sleep-promoting fermented beverage provided in Example 2 for a trial to verify the product's sleep-promoting effect. The test plan is as follows:

[0091] Sixty-two individuals aged 18-65 years with poor sleep quality or varying degrees of insomnia lasting for more than one month were recruited. They were divided into a placebo group and a product trial group, with 31 participants in each group. Each participant was instructed to take either 50ml of the placebo (control group) or the sleep-promoting fermented beverage provided in Example 2 (trial group) daily for one month. The product's sleep-promoting effect was determined using the Pittsburgh Sleep Quality Index score, the Sleep Disorders Rating Scale score, and the Insomnia Severity Index Self-Rating Scale score; higher scores indicated poorer sleep quality. Results are shown in Tables 2-4.

[0092] Table 2 Comparison of Pittsburgh Sleep Quality Index (PSQI) scores of the participants

[0093]

[0094] Table 3 Comparison of Sleep Disorders Rating Scale (SDRS) scores of the participants

[0095]

[0096] Table 4 Comparison of Insomnia Severity Index (ISI) scores among subjects

[0097]

[0098] As shown in Tables 2-4, the differences in the Pittsburgh Sleep Quality Index score, Sleep Disorder Rating Scale score, and Insomnia Severity Index self-rating score before and after the trial of the two groups indicate that the rate of decrease in each index was significantly different after consuming the sleep-promoting fermented beverage provided by this invention. Therefore, this sleep-promoting fermented beverage has a good sleep-promoting effect.

[0099] The above examples are merely illustrative of the present invention and do not constitute a limitation on the scope of protection of the present invention. All designs that are the same as or similar to the present invention are within the scope of protection of the present invention.

Claims

1. A plant complex enriched in gamma-aminobutyric acid, characterized in that: The plant composite includes a plant material and a double-spore mushroom enzyme treatment liquid; the double-spore mushroom enzyme treatment liquid includes double-spore mushrooms, cellulase, yeast, and acetic acid bacteria; the plant material includes quinoa or brown rice; and the preparation method of the plant composite includes the following steps: (1) double-spore mushrooms are crushed and mixed with water to obtain a double-spore mushroom slurry, cellulase is added to the double-spore mushroom slurry, and yeast and acetic acid bacteria are inoculated and fermented to obtain a double-spore mushroom enzyme treatment liquid; (2) the plant material is mixed with the double-spore mushroom enzyme treatment liquid and germinated, and after the germination is completed, the obtained mixture is dried to obtain a plant composite rich in gamma-aminobutyric acid.

2. The GABA-enriched plant complex of claim 1, wherein: The mass ratio of the plant material to the double-spore mushroom enzyme treatment liquid in the plant composite is (2-3):

1.

3. The GABA-enriched plant complex of claim 1, wherein: The double-spore mushroom enzyme treatment liquid includes double-spore mushrooms, cellulase, yeast, and acetic acid bacteria at a mass ratio of 100:(1.5-3.2):(1.8-3.2):(0.6-2.0).

4. The GABA-enriched plant complex of claim 1, wherein: In step (1), the fermentation temperature is 30-32°C, and the fermentation time is 24-30 h.

5. The GABA-enriched plant complex of claim 1, wherein: In step (2), the germination temperature is 25-30°C, and the germination time is 48-72 h.

6. A plant fermentation broth enriched in gamma-aminobutyric acid and soluble polysaccharides, characterized in that: The plant composite rich in gamma-aminobutyric acid includes a plant composite, amylase, papain, Lactobacillus rhamnosus, and Lactobacillus plantarum; the plant composite is as described in any one of claims 1-5.

7. The method for preparing plant fermentation broth as described in claim 6, characterized in that, The method includes the following steps: (1) the plant composite is mixed with water to obtain a composite powder slurry, the pH of the composite powder slurry is adjusted, amylase and papain are added to the composite powder slurry for enzymatic hydrolysis to obtain a primary enzymatic hydrolysis liquid; (2) Lactobacillus rhamnosus and Lactobacillus plantarum are inoculated in the primary enzymatic hydrolysis liquid for fermentation, and the filtrate is obtained by centrifugation to remove the filter residue, thereby obtaining a plant fermentation liquid rich in gamma-aminobutyric acid and soluble polysaccharides.

8. The method for preparing plant fermentation broth as described in claim 7, characterized in that: In step (1), amylase is added to the composite powder slurry for enzymatic hydrolysis for 20-30 min, and then papain is added for enzymatic hydrolysis for 15-30 min to obtain the primary enzymatic hydrolysis liquid.

9. A sleep-promoting fermented beverage, characterized by: The plant fermentation liquid rich in gamma-aminobutyric acid and soluble polysaccharides includes a plant fermentation liquid, Suanzaoren, and lily; the plant fermentation liquid is as described in claim 6.

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