A composition to relieve the stress of skin aging due to heat

Abstract

The application belongs to the field of cosmetics and discloses a composition for relieving skin thermal aging stress. The composition for relieving skin thermal aging stress comprises, in parts by mass, 2-12 parts of silk fibroin, and 10-65 parts of a relieving ferment, wherein the relieving ferment is a combination of eucommia ulmoides, peach resin, cactus and dendrobium, and the mass ratio of eucommia ulmoides, peach resin, cactus and dendrobium is 3-4:3-5:2-3:2-3. The composition provided by the application can improve cell heat shock protection, restore normal expression of proteome induced by thermal stress, and improve sensitive symptoms such as skin photoaging, redness and burning sensation.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, and more specifically to a composition that relieves the stress of skin aging due to heat. Background Technology

[0002] Sunlight can damage the skin, leading to sagging and wrinkles. Its effects on human skin are caused by the combined effects of numerous wavelengths of light, including ultraviolet (UV), visible, and infrared (IR, wavelength 760nm-1mm). Almost half of the solar energy reaching the Earth's surface comes from the infrared wavelength range. Infrared radiation has unique properties; in physics, it is called "thermal rays." It interacts with molecules within tissues, causing them to vibrate and generate heat. After 15-20 minutes of exposure to direct sunlight, the temperature of human skin can rise to 40-43°C. This phenomenon is caused by the thermal effect of infrared radiation and is therefore also known as the thermal effect or warming effect. IR can reach the dermis, penetrating up to 10mm deep, directly affecting blood vessels, lymphatic vessels, nerve endings, and other subcutaneous tissues. Through its thermal effect, it raises skin temperature, causes capillary dilation and congestion, increases epidermal water evaporation, and directly damages the skin. It can also activate TRPV1 receptors through thermal induction, accelerating skin inflammation and aging.

[0003] Infrared radiation and heat, as major environmental factors affecting the skin daily, can induce changes in multiple skin components. These primarily include elastin and collagen, which influence skin aging; secondly, they also have significant regulatory effects on fibronectin and chondroitin sulfate. Mature elastic fibers are composed of elastin and microfibrils. Infrared radiation or heat can cause an imbalance in the expression of these two major macromolecules involved in elastic fiber assembly, ultimately leading to the degradation of normal elastic fibers and an abnormal increase in the expression of amorphous elastic fiber components, resulting in photoaging phenomena such as photosensitive elastic tissue degeneration.

[0004] Most sunscreens and photoprotective products on the market currently target ultraviolet (UV) radiation, and visible light protection is receiving increasing attention, while protection against infrared (IR) and heat has not been given sufficient consideration. However, as mentioned above, the effects of infrared radiation and heat on the skin are long-term and multifaceted, such as skin aging and sensitivity. Therefore, there is an urgent need to develop a cosmetic active ingredient that can alleviate the aging and damage stress caused by infrared radiation and heat on the skin. Summary of the Invention

[0005] The purpose of this invention is to address the thermal aging stress on the skin caused by temperature and light exposure, such as photoaging caused by matrix metalloproteinase overexpression leading to an imbalance in collagen and fibrin-1 expression, as well as skin redness, burning sensation, and other sensitive symptoms caused by increased skin temperature due to thermal radiation, resulting in capillary dilation, congestion, and epidermal moisture evaporation. This invention provides a composition that relieves the thermal aging stress on the skin. This composition can improve cellular heat shock protection, restore the normal expression of the proteome induced by heat stress, and improve photoaging and sensitive symptoms such as redness and burning sensation.

[0006] Natural silk has a pearly luster, a smooth touch, and a protein content exceeding 96%, composed of 18 amino acids. Its structure is very similar to human skin, hence the nickname "second skin." Silk fibroin, also known as silk core protein, is a product obtained after silk degumming. It has good biocompatibility, strong adhesion, and is easily absorbed by the skin. Silk fibroin has a wide range of biological functions, such as antioxidant effects on epidermal cells and protective and repairing effects on damaged skin tissue. In addition, it can reduce reflected light and absorb ultraviolet rays, providing special benefits for protecting the skin and preventing dryness, sunburn, and tanning.

[0007] To achieve the purpose of this invention, the composition for relieving skin heat aging stress of this invention contains, by weight, 2-12 parts of silk protein and 10-65 parts of soothing ferment, wherein the soothing ferment is a combination of Eucommia ulmoides, peach resin, cactus and Dendrobium.

[0008] Furthermore, in some embodiments of the present invention, the mass ratio of Eucommia ulmoides, peach resin, cactus, and Dendrobium is 3-4:3-5:2-3:2-3.

[0009] Preferably, in some embodiments of the present invention, the mass ratio of Eucommia ulmoides, peach resin, cactus, and Dendrobium is 3.5:3.5-5:2-2.5:2-2.5.

[0010] Preferably, in some embodiments of the present invention, the mass ratio of Eucommia ulmoides, peach resin, cactus, and Dendrobium is 3.5:5:2.5:2.5 or 3.5:3.5:2:2.

[0011] Furthermore, in some embodiments of the present invention, the mass ratio of the silk protein to the soothing ferment is 2-8:30-50.

[0012] Furthermore, in some embodiments of the present invention, the mass ratio of the silk protein to the soothing ferment is 50:7, 40:2, or 40:12.

[0013] Furthermore, in some embodiments of the present invention, the preparation method of the silk fibroin is as follows: take silkworm silk and cut it into fragments, use urea solution, degumm it under heating, wash it with deionized water to obtain degummed silkworm silk, dissolve silk fibroin in the Ca(NO3)2-EtOH-H2O system, put the silk fibroin solution into a dialysis bag, dialyze it with deionized water, desalt it, centrifuge the solution to remove impurities, and finally freeze-dry the silk fibroin solution to obtain silk fibroin.

[0014] Preferably, in some embodiments of the present invention, the urea concentration in the preparation method of the silk protein is 8-9 mol / L, the bath ratio is 1:30-50, the heating temperature is 80-90℃, and the degumming time is 2-3 hours.

[0015] Preferably, in some embodiments of the present invention, the Ca(NO3)2-EtOH-H2O bath ratio is 1:45-55, the water bath temperature is 80-90℃, and the dissolution time is 30-50 min in the preparation method of the silk protein.

[0016] Preferably, in some embodiments of the present invention, the dialysis time in the preparation method of the silk core protein is 48-72h, and the impurity is removed by centrifugation at 3500-5000r / min for 3-5min.

[0017] Furthermore, in some embodiments of the present invention, the method for preparing the soothing ferment is as follows: Eucommia ulmoides, peach gum, cactus, and dendrobium are sorted, cleaned, and dried at low temperature, pulverized, weighed according to a certain ratio, and the Eucommia ulmoides, peach gum, cactus, and dendrobium that have been soaked in deionized water are mixed evenly, inoculated with bacterial solution, and after fermentation is completed, ultrasonic extraction, sterilization, and filtration are performed to obtain the product.

[0018] Preferably, in some embodiments of the present invention, the low-temperature drying conditions in the method for preparing the soothing ferment are drying at a temperature below 50°C; the drying method includes, but is not limited to, one or a combination of two of oven drying, freeze drying, and vacuum drying.

[0019] Preferably, in some embodiments of the present invention, the amount of deionized water used in the preparation method of the soothing ferment is 5 to 20 times the total mass of Eucommia ulmoides, peach resin, cactus and Dendrobium.

[0020] Preferably, in some embodiments of the present invention, the inoculated bacterial solution in the preparation method of the soothing ferment includes, but is not limited to, one or two of lactobacillus, lactic acid bacteria, and yeast, and the inoculation amount is 5%-10% of the mass of Eucommia ulmoides, peach resin, cactus, and Dendrobium, with a bacterial content of ≥1.0×10⁻⁶. 11 The concentration of cfu / g was determined by the fermentation temperature of 28-35℃ and the fermentation time of 12-48 hours.

[0021] Preferably, in some embodiments of the present invention, the ultrasonic extraction time in the preparation method of the soothing ferment is 30-60 min.

[0022] Preferably, in some embodiments of the present invention, the microfiltration membrane used for filtration in the method for preparing the soothing ferment is a microfiltration membrane with a pore size range of 0.1-10 μm, and more preferably a microfiltration membrane with a pore size of 10 μm, 0.45 μm and 0.2 μm.

[0023] On the other hand, the present invention also provides a method for preparing the aforementioned composition for relieving skin heat aging stress, the method comprising:

[0024] (1) Preparation of soothing fermentation product;

[0025] (2) Preparation of silk core protein;

[0026] (3) Mix the silk protein and the soothing ferment in a certain proportion to obtain the composition.

[0027] In another aspect, the present invention also provides a cosmetic product comprising the aforementioned composition of the present invention for relieving skin stress caused by heat aging.

[0028] Preferably, in some embodiments of the present invention, the cosmetics include, but are not limited to, aqueous solutions, emulsions, sprays, creams, masks, and foundations.

[0029] Compared with the prior art, the advantages of the present invention are as follows:

[0030] (1) At the same total concentration, the cell thermal stress protection ability of the composition provided by the present invention is higher than that of the single component. Under thermal stress, the cell survival rate after the composition is treated is higher, indicating that the combination of filament protein and soothing ferment in the present invention can synergistically enhance the cell thermal shock protection.

[0031] (2) The silk fibroin described in this invention has an amino acid composition similar to that of human skin and is a homologous protein to human epidermal protein. It has a strong affinity for the skin and can participate in the repair of skin tissue. It can form a protective film on the surface of the skin and hair. The polysaccharide components in the soothing ferment described in this invention work synergistically with the silk fibroin to form a skin protective film that can absorb ultraviolet rays and prevent photoaging of the skin. Attached Figure Description

[0032] Figure 1 This describes the promoting effect of embodiments 6 and 8 of the present invention on HSP27 gene expression;

[0033] Figure 2 This demonstrates the inhibitory effect of Examples 6 and 8 of the present invention on MMP-1 gene expression;

[0034] Figure 3 This is a graph showing the change in skin elasticity before and after using the composition described in this invention. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. It should be understood that the following description is merely illustrative and not intended to limit the invention.

[0036] The terms “comprising,” “including,” “having,” “containing,” or any other variations thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that includes the listed elements is not necessarily limited to those elements, but may include other elements not expressly listed or elements inherent to such composition, step, method, article, or apparatus.

[0037] When a quantity, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper and lower preferred values, this should be understood as specifically disclosing all ranges formed by any pair of any upper or preferred value with any lower or preferred value, regardless of whether the range is disclosed individually. For example, when the range “1 to 5” is disclosed, the described range should be interpreted as including the ranges “1 to 4”, “1 to 3”, “1 to 2”, “1 to 2 and 4 to 5”, “1 to 3 and 5”, etc. When numerical ranges are described herein, unless otherwise stated, the range is intended to include its endpoints and all integers and fractions within that range.

[0038] The indefinite articles “a” and “an” preceding an element or component of this invention do not impose any limitation on the quantity (i.e., number of times) of the element or component. Therefore, “an” or “a” should be interpreted as including one or at least one, and the singular form of an element or component also includes the plural form, unless the quantity clearly refers only to the singular form.

[0039] Furthermore, the terms "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., described below refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms are not necessarily directed at the same embodiment or example. Moreover, the technical features involved in the various embodiments of the present invention can be combined with each other as long as they do not conflict with each other.

[0040] Unless otherwise specified, all raw materials and equipment used in this application are commonly used in the field and are derived from commercially available products. Unless otherwise specified, all methods used in this application are conventional methods in the field.

[0041] There are many other feasible technical solutions in this application, which will not be listed here. All technical solutions claimed in the claims of this application are feasible.

[0042] Example 1

[0043] In the embodiments and comparative examples of the present invention, the composition for relieving the stress of skin photoaging is prepared using the following process:

[0044] (1) After sorting and cleaning Eucommia ulmoides, peach gum, cactus and dendrobium and drying them at a temperature below 50°C, weigh them according to a mass ratio of 3.5:5:2.5:2.5;

[0045] (2) After crushing Eucommia ulmoides, peach gum, cactus and dendrobium, add 20 times their total weight of deionized water to soak and stir for 2 hours;

[0046] (3) Inoculate (2) with 10% Lactobacillus bacterial solution and ferment at 35°C for 48 hours with stirring; wherein the bacterial count in the solution is ≥1.0×10 11 cfu / g;

[0047] (4) After fermentation, ultrasonic extraction for 20 min is completed, followed by filtration, sterilization, and centrifugation of the filtrate to obtain the supernatant.

[0048] (5) The supernatant obtained in (4) is filtered sequentially through microfiltration membranes with pore sizes of 10 μm, 0.45 μm and 0.2 μm, and the filtrate is collected to obtain the soothing fermentation product.

[0049] (6) Take silkworm silk and cut it into pieces about 1cm long. 2 The fragments were degummed using a 9 mol / L urea solution as the degumming agent at a 1:50 bath ratio at 90°C for 3 hours, and then washed with deionized water to obtain crude silk fibroin.

[0050] (7) Using Ca(NO3)2-EtOH-H2O (molar ratio of 1:2:8) as the dissolving solution, with a bath ratio of 1:50, the silk fibroin solution was dissolved for 30 minutes at a water bath temperature of 90℃. Then, the solution was placed in a dialysis bag and dialyzed with deionized water for 72 hours.

[0051] (8) Centrifuge the desalted silk fibroin solution at 5000 r / min for 3 min to remove impurities, and then freeze dry to obtain silk fibroin.

[0052] Table 1 shows the composition ratios of each composition in Examples 1-9 and Comparative Examples 1-2 (the composition ratios of silk protein and soothing ferment are by mass, and the total mass of the compositions in each example and comparative example is consistent). The compositions of each example and comparative example were prepared according to the above method based on the raw material composition in Table 1, and the efficacy of the obtained compositions was tested.

[0053] Table 1

[0054]

[0055]

[0056] Experimental Example 1

[0057] Cytotoxicity assessment assays:

[0058] The MTT assay is a widely used experimental method for detecting cytotoxicity or cell proliferation by measuring the number of viable cells. Its principle is that succinate dehydrogenase in the mitochondria of viable cells reduces the water-soluble yellow salt MTT (3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide) to a water-insoluble blue formazan, which is then deposited in the cells. Dead cells do not have this function. The resulting crystalline formazan is generally dissolved in DMSO (dimethyl sulfoxide) and the absorbance is measured. The specific experimental method involves preparing a 96-well plate with a 1 x 10⁻⁶ well. 4 Cells were seeded at a density of 100 μl each of DMEM medium containing 10% bovine serum and keratinocytes (HaCaT) in 24 wells and then cultured in serum-free medium. Different concentrations of the combined solution were added to the serum-free medium for treatment, followed by culturing at 37°C for 24 hours. The heat-treated cells were then subjected to a thermostress of 45°C for 3.5 hours, followed by no further heat treatment and continued culturing at 37°C for 24 hours. Afterward, the medium was removed, and the cells were treated with 20 μl of MTT solution and reacted at 37°C for 2 hours. 200 μl of isopropanol was added to the cells after removing the MTT solution, and the mixture was gently shaken for 30 min to completely dissolve the formazan crystals. The absorbance was measured at 570 nm, and cell viability was calculated using the following formula.

[0059]

[0060] The blank group was tested without the composition. Table 2 shows the cytotoxicity results of the compositions obtained in Examples 1-9 and Comparative Examples 1-2.

[0061] As shown in Table 2:

[0062] 1) After heat treatment at 45°C, the cell survival rate decreased significantly, proving that heat stress can significantly inhibit cell viability. However, the cell survival rate of cells treated in Examples 1-9 and Comparative Examples 1-2 increased significantly, and the cell survival rate of Examples 1-9 was higher than that of Comparative Examples 1-2, proving that filoin and soothing ferment can protect cells from heat stress damage, and that the two can synergistically enhance the cells' ability to resist heat stress.

[0063] 2) It was also found that the cell survival rate of Examples 6, 8 and 9 was the highest, proving that the cells had the strongest ability to resist thermal stress damage after being treated by Examples 6, 8 and 9.

[0064] Table 2

[0065]

[0066]

[0067] By changing the proportions of the components in the soothing ferment, it was found that when the mass ratio of Eucommia ulmoides, peach resin, cactus, and Dendrobium was 3-4:3-5:2-3:2-3, it had no significant effect on the effect of the combination of the silk core protein and the soothing ferment in this invention.

[0068] Experimental Example 2

[0069] IR-induced HSP27 gene expression assay:

[0070] Heat shock proteins (HSPs), also known as stress proteins, are involved in cell damage and repair. They belong to a specific family of chaperone proteins and are upregulated during various types of cellular stress to maintain and circulate the proteome. Heat shock protein 27 (HSP27) is an important protein involved in cell growth, autophagy, apoptosis, drug resistance, and is related to tumorigenesis and metastasis. High expression of HSP27 has a protective effect after the body is stimulated by various internal and external environmental factors (heat stress, oxidative stress, organic toxins, etc.). The level of HSP27 expression and its phosphorylation state are key factors in cellular resistance to heat damage. This experimental protocol uses IR-stimulated keratinocytes. After treatment with the analyte, changes in HSP27 gene expression are detected to evaluate the efficacy of the test sample against heat aging stress.

[0071] HaCaT cells in the logarithmic growth phase were harvested, the culture medium was discarded, and the cells were washed twice with PBS. 0.25% trypsin was added and the cells were incubated at room temperature for 5 minutes to stop digestion. The cells were then centrifuged at 1000 rpm for 3 minutes, resuspended in complete DMEM medium, and counted. The cell concentration was adjusted to 1.5 × 10⁻⁶ cells / mL. 51 ml was added to each well of a 48-well plate. After culturing for 24 h, the compositions of Example 6 and Example 8, as well as the blank group (no sample added), were added respectively. After culturing for another 24 h, cellular RNA was extracted, and the HSP27 expression level of each group was detected. Relative expression levels were analyzed according to the 2-ΔΔct method. The calculation method is as follows:

[0072] △CT experimental group = CT target gene - CT internal reference gene, △CT control group = CT target gene - CT internal reference gene, △△CT = △CT experimental group - △CT control group;

[0073] The relative expression level of the target gene = 2 －△△CT

[0074] Figure 1 The results shown are those of the HSP27 gene expression level assay in Examples 6 and 8, representing the blank control group. Figure 1 It can be seen that after pretreatment in Examples 6 and 8, the expression level of HSP27 was significantly increased compared with the untreated blank group, indicating that the composition of the present invention can enhance the expression of HSP27 gene, maintain the expression of healthy proteome, and resist the thermal aging stress of skin cells.

[0075] Experimental Example 3

[0076] Matrix metalloproteinase 1 gene expression assay:

[0077] Matrix metalloproteinase-1 (MMP-1) is the main enzyme responsible for collagen degradation. Its expression is upregulated after exposure to sunlight or infrared radiation. Since collagen degradation is an important indicator of photoaging, MMP-1 expression is used as an indicator to evaluate the effect of anti-photoaging / thermal aging.

[0078] This experimental protocol is based on IR-stimulated fibroblasts. After the test substance is applied, the anti-thermal aging efficacy of the test sample is evaluated by detecting changes in MMP-1 gene expression.

[0079] After cell culture, the cells were divided into a blank control group, a model group, and a sample group. The blank control group was directly replaced with DMEM low-glucose medium. The model group and the sample group were induced by IR, and then the model group was replaced with DMEM low-glucose medium. The sample group was added according to Examples 6 and 8, and then replaced with DMEM low-glucose medium, and cultured for another 24 hours. The medium was discarded, and the cells were washed twice with PBS. RNA was extracted, and PCR was performed to detect the expression level of the MMP-1 gene. The relative expression level was analyzed according to the 2-ΔΔct method. The calculation method is as follows:

[0080] △CT experimental group = CT target gene - CT internal reference gene, △CT control group = CT target gene - CT internal reference gene, △△CT = △CT experimental group - △CT control group;

[0081] The relative expression level of the target gene = 2 －△△CT

[0082] Figure 2 The results shown are the MMP-1 gene expression levels in the blank group, model group, and Examples 6 and 8. Figure 2 It was found that, compared to the blank group, the expression level of MMP-1 in the model group after IR irradiation was significantly increased. This indicates that IR irradiation can upregulate the expression of matrix metalloproteinase MMP-1, leading to excessive collagen degradation. In Examples 6 and 8, compared to the model group, the expression of MMP-1 was significantly reduced. This demonstrates that the composition can resist the thermal aging phenomenon caused by IR on the skin.

[0083] Experiment Example 4

[0084] Safety patch test:

[0085] Add 20 μl of the test solution to the patch applicator, with a blank control (pure water) in the control well. Apply the patch applicator containing the test substance to the flexor side of the subject's forearm, gently pressing it with the palm of your hand to ensure even application to the skin, and leave it on for 24 hours. Observe skin irritation and sensitization according to Table 3 at 30 min, 24 h, and 48 h after removing the patch applicator, and record the results. The grading criteria for skin reaction in the closed patch test are shown in Table 3.

[0086] Table 3

[0087]

[0088] Experimental Results: Human skin patch tests were conducted on the compositions of Examples 6 and 8, and the results are shown in Table 4. The human skin patch test results showed no adverse skin reactions in 30 participants.

[0089] Table 4

[0090]

[0091] Experimental Example 5

[0092] 1) Cooling effect test:

[0093] Thirty female volunteers aged 25 to 45 were selected. Ten volunteers applied the composition of Example 6, ten volunteers applied the composition of Example 8, and the remaining ten volunteers applied a placebo (the composition was identical to that of Examples 6 and 8 except it did not contain the original composition). The composition was applied to the entire face at a dosage of 2 mg / cm².2 Use morning and evening for two consecutive weeks. The cooling effect of the composition was evaluated using a skin surface temperature-thermal imaging camera (FLIR T450sc, USA) on day 0 and after two weeks.

[0094] The experimental results are shown in Table 5. After comparing the blank group with the emulsions containing Examples 6 and 8, it was found that the composition of the present invention has a very excellent cooling effect.

[0095] Table 5

[0096] Group Before (°C) 2 weeks (°C) 2 weeks after IR (°C) Blank group 34.5±0.32 34.2±0.34 37.1±0.26 Example 6 34.2±0.18 32.8±0.22 34.4±0.30 Example 8 34.6±0.28 32.5±0.12 35.0±0.24

[0097] 2) Skin elasticity test:

[0098] Continue using for 14 days, and use instruments to test the rebound ratio R2 (AU) before use, 2 weeks, and 4 weeks later. R2: the ratio of the skin's rebound amount without negative pressure to the maximum stretch amount with negative pressure. The closer the ratio is to 1, the better the skin elasticity. The results are shown below.

[0099] Experimental results: See [link to results] Figure 3 The compositions in Examples 6 and 8 showed significantly improved skin elasticity compared to the control group. After 4 weeks of use, skin elasticity increased by 11.4% and 11.0% respectively compared to before use.

[0100] Those skilled in the art will readily understand that the above description is merely an embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A composition for soothing skin stress from heat aging, characterized by, The composition for relieving skin thermal aging stress comprises 2-12 parts of silk fibroin, 10-65 parts of relieving ferment, wherein the relieving ferment is a combination of Eucommia ulmoides, peach resin, cactus and dendrobium; The preparation method of the silk fibroin comprises the following steps: cutting silk into pieces, degumming the silk under heating by using urea solution, washing the silk with deionized water to obtain crude silk fibroin, dissolving the crude silk fibroin in a Ca(NO3)2-EtOH-H2O system, dialyzing the silk fibroin solution with deionized water, centrifuging the solution to remove impurities, and finally freeze-drying the silk fibroin solution to obtain the silk fibroin. The preparation method of the relieving ferment comprises the following steps: sorting, cleaning and low-temperature drying Eucommia ulmoides, peach resin, cactus and dendrobium, crushing the Eucommia ulmoides, peach resin, cactus and dendrobium, immersing the Eucommia ulmoides, peach resin, cactus and dendrobium in deionized water, inoculating bacteria, fermenting, ultrasonic extraction, sterilization, and filtration. The inoculated bacteria in the preparation method of the relieving ferment are lactic acid bacteria.

2. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, The mass ratio of the Eucommia ulmoides, peach resin, cactus and dendrobium is 3-4:3-5:2-3:2-3.

3. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, The mass ratio of the Eucommia ulmoides, peach resin, cactus and dendrobium is 3.5:3.5-5:2-2.5:2-2.

5.

4. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, The mass ratio of the Eucommia ulmoides, peach resin, cactus and dendrobium is 3.5:5:2.5:2.5 or 3.5:3.5:2:

2.

5. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, The mass ratio of the silk fibroin and the relieving ferment is 2-8:30-50.

6. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, In the preparation method of the silk fibroin, the urea concentration is 8-9 mol / L, the bath ratio is 1:30-50, the heating temperature is 80-90℃, and the degumming time is 2-3 hours.

7. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, In the preparation method of the silk fibroin, the Ca(NO3)2-EtOH-H2O bath ratio is 1:45-55, the water bath temperature is 80-90℃, and the dissolving time is 30-50 min.

8. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, In the preparation method of the silk fibroin, the dialysis time is 48-72 h, the centrifugation is performed at 3500-5000 r / min for 3-5 min.

9. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, In the preparation method of the relieving ferment, the low-temperature drying is performed at a temperature lower than 50℃; and the drying method includes but is not limited to one or a combination of oven drying, freeze drying and vacuum drying.

10. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, In the preparation method of the relieving ferment, the amount of deionized water is 5-20 times the total mass of the Eucommia ulmoides, peach resin, cactus and dendrobium.

11. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, The inoculation amount of the bacteria liquid in the preparation method of the soothing ferment is 5-10% of the mass of eucommia ulmoides, peach resin, cactus and dendrobium, the bacteria content is ≥1.0×10 11 Cfu / g, the fermentation temperature is 28-35℃, and the fermentation time is 12-48h.

12. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, In the preparation method of the relieving ferment, the ultrasonic extraction time is 30-60 min.

13. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, In the preparation method of the relieving ferment, the microfiltration membrane with a pore size ranging from 0.1 to 10 μm is used for filtration.

14. The composition to relieve skin heat-aging stress according to claim 1, characterized in that, In the preparation method of the relieving ferment, the microfiltration membranes with pore sizes of 10 μm, 0.45 μm and 0.2 μm are used for filtration in sequence.

15. A method of preparing the composition for soothing skin stress from heat aging according to any one of claims 1 to 14, characterized in that, The method comprises the following steps: (1) preparing the relieving ferment; (2) preparing the silk fibroin; (3) mixing the silk fibroin and the relieving ferment according to a certain proportion to obtain the composition.

16. A cosmetic product, characterized by, The cosmetic product comprising the composition for relieving the stress of skin thermal aging of any one of claims 1-14, wherein the cosmetic product is an aqueous agent, an emulsion, a spray, a cream, a mask, or a foundation.

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