A long-acting essence milk with the effect of improving sensitive redness and a preparation method thereof

Abstract

The present application relates to a kind of long-acting improvement sensitive red effect of shake essence cream and its preparation method, belong to cosmetic technical field.The long-acting improvement sensitive red effect of shake essence cream in the present application, including following component: repair oil 5~25%, mannose-based erythritol fat 0.05~0.2%, recombinant human source IV type collagen 0.05~0.1%, serum albumin 0.05~0.1%, slender avens extract 1~5%, humectant 2~10%, soothing agent 1~5%, preservative 0.1~2%, water is supplemented to 100%.The shake essence cream of the present application is prepared by scientific proportioning and specific process, has good long-acting source effect of improving sensitive red, and preparation process is simple, stability is good, dosage form is novel, can provide good skin care experience for consumer, has very big market prospect.

Description

Technical Field

[0001] This invention relates to a shake-to-make essence lotion with a long-lasting effect in improving sensitive skin and redness, and its preparation method, belonging to the field of cosmetic technology. Background Technology

[0002] Sensitive skin specifically refers to a hyperreactive state of the skin under physiological or pathological conditions, primarily affecting the face. Clinically, it manifests as subjective symptoms such as burning, stinging, itching, and tightness when stimulated by physical, chemical, or psychological factors, with or without objective signs like erythema, scaling, and telangiectasia. Research shows that redness is a major concern for those with sensitive skin. Redness indicates that the skin is in a sensitive and fragile state, overreacting to external stimuli, leading to neurogenic or immune-mediated inflammation, which in turn causes telangiectasia, resulting in redness on the skin's surface. If this dilation is not effectively controlled in the short term, it may develop into abnormal telangiectasia, forming a network of red blood vessels, which not only affects the skin's appearance but may also increase skin sensitivity and other skin problems.

[0003] Products on the market that relieve skin redness are typically in the form of toners, lotions, or creams, with relatively simple formulations. Existing two-phase serums are usually simple water-oil dual-phase products, which can lead to rapid separation, difficulty in evenly dispensing the liquid, and slow absorption. Some products add small amounts of emulsifiers, resulting in unclear water-oil interface and opaque, cloudy water-oil phases, affecting the user experience. On the other hand, the efficacy logic of products relieving skin redness on the market is usually as follows: first, to relieve / inhibit inflammation and reduce skin discomfort; second, to repair the skin barrier by choosing skin-friendly or sebum-like ingredients to repair damaged redness and strengthen the barrier function; and finally, to increase skin hydration and moisturize, preventing dryness, redness, and stinging. However, these products usually work well when first used, but the redness problem tends to recur later. They cannot provide long-term repair for sensitive skin redness and bring the skin to a stable and ideal state. The reason is that this method cannot solve the problem of skin sensitivity at its root. It ignores the damage and repair of cells and extracellular matrix caused by inflammation and swelling during the process of skin sensitivity and redness, especially the damage to the basement membrane caused by inflammation.

[0004] The basement membrane, as the connecting layer between the dermis and epidermis, plays a crucial role in bridging these two layers. When damaged, the basement membrane cannot function properly, affecting the structural support of the epidermis and dermis, as well as the transport of nutrients and moisture. The epidermis cannot receive normal nutrient supply, and its own keratinization process is also affected, ultimately leading to a vicious cycle of impaired barrier function of the outermost protective layer of the skin—increased inflammation—basement membrane damage—and sensitivity and redness, preventing a fundamental solution to the problem of sensitive and red skin. Summary of the Invention

[0005] To address the shortcomings and deficiencies of existing technologies, this invention provides a shake-to-make essence lotion with a long-lasting effect in improving sensitive skin and redness, and its preparation method. Through screening, analysis, comparison, and verification of the structure and efficacy of numerous raw material components, this invention discovers that a shake-to-make essence lotion with a long-lasting effect in improving sensitive skin and redness can be prepared by scientifically proportioning and using a specific process with repairing oils, mannosyl erythritol esters, recombinant humanized type IV collagen, serum albumin, Geranium wilfordii extract, moisturizers, soothing agents, and preservatives.

[0006] The technical solution of the present invention is as follows:

[0007] A shake-to-apply serum with long-lasting effects in improving sensitive skin and redness, comprising the following components by weight percentage:

[0008] Repairing oils 5-25%, mannosyl erythritol ester 0.05-0.2%, recombinant humanized type IV collagen 0.05-0.1%, serum albumin 0.05-0.1%, slender geranium extract 1-5%, moisturizer 2-10%, soothing agent 1-5%, preservative 0.1-2%, water replenished to 100%.

[0009] According to a preferred embodiment of the present invention, the repairing oil includes, but is not limited to, one or more of the following components: squalane, meadowfoam seed oil, jojoba seed oil, camellia seed oil, olive oil, avocado oil, marula oil, burdock seed oil, coconut oil-caprylate / capric acid ester, and isononyl isononanoate.

[0010] More preferably, the repairing oil is a composition of 1-5% squalane, 1-3% meadowfoam seed oil, 2-5% jojoba seed oil, and 5-10% coconut oil-caprylate / capric acid ester.

[0011] In this invention, the mannosyl erythritol ester is a type of glycolipid.

[0012] According to a preferred embodiment of the present invention, the amino acid sequence of the recombinant humanized type IV collagen is shown in SEQ ID NO.1, and it is prepared according to the method described in patent 202410255649.1.

[0013] According to a preferred embodiment of the present invention, the serum albumin is plant-derived recombinant serum albumin.

[0014] According to a preferred embodiment of the present invention, the mass ratio of the recombinant humanized type IV collagen to serum albumin is 1:1.

[0015] In this invention, the Geranium slenderum extract is an aqueous solution composed of butylene glycol, water, and Geranium slenderum extract in a mass ratio of 49.5:49.5:1.

[0016] According to a preferred embodiment of the present invention, the moisturizer includes, but is not limited to, a combination of one or more of the following: pentylene glycol, 1,3-butanediol, glycerin, xylitol glucoside, dehydrated xylitol, tremella fuciformis extract, β-glucan, D-panthenol, xylitol, sodium hyaluronate, and recombinant humanized type III collagen.

[0017] More preferably, the moisturizer is a composition of 1-3% 1,3-butanediol, 1-3% glycerin, 0.5-2% D-panthenol, and 0.05-0.1% sodium hyaluronate.

[0018] According to a preferred embodiment of the present invention, all soothing agents are -SOFT 1609. One or more of the following components: oat kernel extract, bisabolol, dipotassium glycyrrhizate, and horse chestnut extract.

[0019] More preferably, the soothing agent is A composition of SOFT 1.0-3.0%, dipotassium glycyrrhizate 0.1-0.2%, and horse chestnut (AESCULUS CHINENSIS) extract 0.2-1%.

[0020] In this invention, the -SOFT is composed of propylene glycol, ALBATRELLUS CONFLUENS extract, and citric acid in a mass ratio of 96.5:3:0.5.

[0021] According to a preferred embodiment of the present invention, the preservative comprises a combination of one or more of the following components: p-hydroxyacetophenone, hexanediol, ethylhexylglycerin, and octyl glycol.

[0022] More preferably, the preservative is a composition of 0.3-0.5% hexanediol and 0.3-0.5% p-hydroxyacetophenone.

[0023] The preparation method of the above-mentioned shake-to-enhance serum with long-lasting effect in improving sensitive skin and redness includes the following steps:

[0024] S1. Mix mannosyl erythritol with repair oil, heat to dissolve into a transparent liquid and stir evenly. The heating temperature is 70-80℃.

[0025] S2. Cool the dissolved oil phase from step S1 to 35-42°C;

[0026] S3. Heat the water to 65-70℃, add the preservative, and stir until dissolved and evenly mixed;

[0027] S4. When the aqueous phase stirred in step S3 is cooled to 35-42°C, add soothing agent, moisturizer, recombinant humanized type IV collagen, serum albumin, and geranium extract, dissolve and stir evenly to obtain a transparent and homogeneous aqueous solution.

[0028] S5. Add the oil phase from step S2 to the aqueous phase solution from step S4 to form the shake-to-use essence emulsion. Shake before use until it becomes an opaque and uniform emulsion.

[0029] According to a preferred embodiment of the present invention, the stirring conditions in step S1 are: stirring speed of 600-800 rpm and stirring time of 5-10 min.

[0030] According to a preferred embodiment of the present invention, the stirring conditions in step S3 are: stirring speed of 600-800 rpm and stirring time of 20-30 min.

[0031] According to a preferred embodiment of the present invention, the stirring conditions in step S4 are: stirring speed of 600-1000 rpm and stirring time of 20-30 min.

[0032] The repairing oil components in this invention can rebuild the skin's surface lipid film when the stratum corneum barrier function is insufficient, effectively isolating external stimuli, and also preventing skin moisture loss, thus protecting the skin and repairing the skin's lipid film barrier.

[0033] The mannosyl erythritol ester in this invention is a natural, multifunctional glycolipid produced by Pseudomonas aeruginosa metabolism. Compared to ionic emulsifiers such as sophorolipids and rhamnolipids, mannosyl erythritol ester is gentler and less irritating to the skin. Furthermore, in this patented invention, mannosyl erythritol ester can also provide instant emulsification. Unlike ordinary two-layer water-oil essences that separate too quickly and have uneven distribution after shaking, water-oil essences containing mannosyl erythritol ester form a uniform emulsion after shaking the water and oil phases, without separation within a short time. This ensures that the water and oil phases can be evenly extracted during product use, and the product automatically reverts to a clear, two-layer oil-water essence texture later, providing consumers with a richer skincare experience. Secondly, mannosyl erythritol ester has anti-inflammatory bioactivity and promotes the absorption of active ingredients, enhancing product efficacy and skin feel.

[0034] The recombinant humanized type IV collagen in this invention can be obtained through fermentation. Its amino acid sequence is shown in SEQ ID NO.1. It can be prepared according to the method described in patent 202410255649.1. This raw material has a repairing and anti-wrinkle effect when used in skin care products.

[0035] The serum albumin in this invention is a plant-derived recombinant serum albumin, which carries no risk of viral infection from animal or microbial sources. When used in skincare products, it has protective, stabilizing, and active ingredient-carrying effects, as well as antioxidant and oxidative damage reduction effects.

[0036] The slender geranium extract in this invention can improve skin elasticity and repair the basement membrane by inhibiting the degradation of type I and type IV collagen in the skin.

[0037] This invention, by adding recombinant humanized type IV collagen, serum albumin, and geranium extract in a specific ratio, can synergistically repair the skin's basement membrane, thereby achieving long-term relief of skin sensitivity and redness and maintaining skin stability.

[0038] The soothing ingredients in this invention have the effects of inhibiting the expression of inflammatory factors, inhibiting capillary permeability, and blocking the TRPV-1 receptor binding site, thus providing short-term relief for skin discomfort and sensitive redness.

[0039] The moisturizing ingredients in this invention can improve dry skin, increase skin moisture content, and keep the skin hydrated and plump.

[0040] The preservative components in this invention have excellent broad-spectrum and highly effective antifungal effects, and can replace traditional phenoxyethanol and methylparaben preservatives, offering higher safety and being gentle and non-irritating to the skin.

[0041] Beneficial effects:

[0042] This invention provides a shake-to-use essence lotion with a long-lasting effect in improving sensitive skin redness. This essence lotion is prepared by scientifically proportioning and using a specific process to produce repairing oils, mannosyl erythritol lipids, recombinant humanized type IV collagen, serum albumin, geranium extract, moisturizers, soothing agents, and preservatives. It has a good long-lasting effect in improving redness in sensitive skin, and the preparation process is simple, stable, and novel in formulation. It can provide consumers with a good skin care experience and has a very large market prospect. Attached Figure Description

[0043] Figure 1 Image of the shake-to-enriched essence product prepared in Example 2.

[0044] Figure 2 The image shows the effect of the shake-up essence prepared in Example 2 after being shaken.

[0045] Figure 3 Images showing changes in facial redness before use, 14 days after use (D14), and 14 days after discontinuation (D28) in Example 2. Detailed Implementation

[0046] The technical solution of the present invention will be further described below with reference to the embodiments, but the scope of protection of the present invention is not limited thereto.

[0047] Raw materials and their sources involved in the examples:

[0048] Table 1. Raw materials and their sources involved in the examples

[0049]

[0050] The amino acid sequence of the recombinant humanized type IV collagen is shown in SEQ ID NO.1. Specifically, it can be prepared according to the patent (application number 202410255649.1), that is, codon optimization of the coding gene is performed based on the codon preference of *E. coli*. The nucleotide sequence of the codon-optimized coding gene is shown in SEQ ID NO.2. The coding gene is inserted into the expression vector pET28a to construct a recombinant plasmid. The recombinant plasmid is transformed into *E. coli* BL21(DE3) to construct a recombinant strain. The recombinant strain is cultured to the logarithmic growth phase, and expression is induced by IPTG. After cell disruption and purification, the recombinant humanized type IV collagen is obtained. The above-mentioned *Geranium slenderum* extract is an aqueous solution composed of butylene glycol, water, and *Geranium slenderum* extract in a mass ratio of 49.5:49.5:1. -SOFT is composed of propylene glycol, ALBATRELLUS CONFLUENS extract, and citric acid in a mass ratio of 96.5:3:0.5.

[0051] The raw material composition and proportions of the shake-to-enrich essences in the examples and comparative examples are shown in Table 2, all of which are mass percentages.

[0052] Table 2. Raw material composition and proportions of the shake-to-enrich emulsions in the examples and comparative examples

[0053]

[0054] Note: Comparative Example 1 replaces mannosyl erythritol ester with an equal amount of sophorolipid, and Comparative Example 2 replaces mannosyl erythritol ester with an equal amount of PEG-6 caprylic / capric glyceride.

[0055] Examples 1-3:

[0056] The shake-to-enhance serums described in Examples 1-3, which have a long-lasting effect in improving sensitive skin and redness, have the following raw material composition and proportions as shown in Table 2. Their preparation method includes the following steps:

[0057] S1. Mix mannosyl erythritol ester with the repair oil composition, heat to dissolve into a transparent liquid and stir evenly at a stirring speed of 700 rpm for 8 min and a heating temperature of 75℃.

[0058] S2. Cool the dissolved oil phase from step S1 to 38°C;

[0059] S3. Heat water to 68°C, add the preservative composition, and dissolve and stir evenly at a stirring speed of 700 rpm for 25 minutes.

[0060] S4. When the aqueous phase stirred in step S3 is cooled to 38°C, add the soothing agent composition, moisturizing agent composition, recombinant humanized type IV collagen, serum albumin, and geranium extract. Dissolve and stir evenly at 800 rpm for 25 min to obtain a transparent and homogeneous aqueous solution.

[0061] S5. Add the oil phase from step S2 to the aqueous phase from step S4 to obtain the shake-to-use essence emulsion. Shake before use until it becomes an opaque and uniform emulsion.

[0062] Among them, the shake-to-enrich emulsion product prepared in Example 2 is as follows: Figure 1 As shown, the effect after shaking is as follows: Figure 2 As shown.

[0063] Comparative Example 1: (Sophorolipids instead of mannosyl erythritol esters)

[0064] A shake-to-enriched essence, the composition and proportions of which are shown in Table 2, differs from Example 2 in that mannosyl erythritol ester is replaced with an equal amount of sophorolipid. Its preparation method includes the following steps:

[0065] S1. Mix the sophoryl ester with the repair oil composition, heat to dissolve into a transparent liquid and stir evenly at a stirring speed of 700 rpm for 8 minutes and a heating temperature of 75°C.

[0066] S2. Cool the dissolved oil phase from step S1 to 38°C;

[0067] S3. Heat water to 68°C, add the preservative composition, and dissolve and stir evenly at a stirring speed of 700 rpm for 25 minutes.

[0068] S4. When the aqueous phase stirred in step S3 is cooled to 38°C, add the soothing agent composition, moisturizing agent composition, recombinant humanized type IV collagen, serum albumin, and geranium extract. Dissolve and stir evenly at 800 rpm for 25 min to obtain a transparent and homogeneous aqueous solution.

[0069] S5. Add the oil phase from step S2 to the aqueous phase from step S4 to obtain the shake-to-use essence emulsion. Shake before use until it becomes an opaque and uniform emulsion.

[0070] Comparative Example 2: (PEG-6 caprylic / capric glyceride instead of mannosyl erythritol ester)

[0071] A shake-to-enriched essence, the composition and proportions of which are shown in Table 2, differs from Example 2 in that mannosyl erythritol ester is replaced with an equal amount of PEG-6 caprylic / capric glyceride. Its preparation method includes the following steps:

[0072] S1. Mix PEG-6 caprylic / capric glyceride with the repair oil composition, heat to dissolve into a transparent liquid and stir evenly at a stirring speed of 700 rpm for 8 min and a heating temperature of 75℃.

[0073] S2. Cool the dissolved oil phase from step S1 to 38°C;

[0074] S3. Heat water to 68°C, add the preservative composition, and dissolve and stir evenly at a stirring speed of 700 rpm for 25 minutes.

[0075] S4. When the aqueous phase stirred in step S3 is cooled to 38°C, add the soothing agent composition, moisturizing agent composition, recombinant humanized type IV collagen, serum albumin, and geranium extract. Dissolve and stir evenly at 800 rpm for 25 min to obtain a transparent and homogeneous aqueous solution.

[0076] S5. Add the oil phase from step S2 to the aqueous phase from step S4 to obtain the shake-to-use essence emulsion. Shake before use until it becomes an opaque and uniform emulsion.

[0077] Comparative Example 3: (Mannosyl erythritol ester)

[0078] A shake-to-enriched essence, the composition and proportions of which are shown in Table 2, differs from Example 2 in that it does not contain mannosyl erythritol ester. Its preparation method includes the following steps:

[0079] S1. Mix the repair oil composition, heat to a clear liquid and stir evenly at a stirring speed of 700 rpm for 8 minutes and a heating temperature of 75°C.

[0080] S2. Cool the dissolved oil phase from step S1 to 38°C;

[0081] S3. Heat water to 68°C, add the preservative composition, and dissolve and stir evenly at a stirring speed of 700 rpm for 25 minutes.

[0082] S4. When the aqueous phase stirred in step S3 is cooled to 38°C, add the soothing agent composition, moisturizing agent composition, recombinant humanized type IV collagen, serum albumin, and geranium extract. Dissolve and stir evenly at 800 rpm for 25 min to obtain a transparent and homogeneous aqueous solution.

[0083] S5. Add the oil phase from step S2 to the aqueous phase from step S4 to obtain the shake-to-use essence emulsion. Shake before use until it becomes an opaque and uniform emulsion.

[0084] Comparative Example 4: (Without recombinant humanized type IV collagen)

[0085] A shake-to-enriched essence, the composition and proportions of which are shown in Table 2, differs from Example 2 in that it does not contain recombinant humanized type IV collagen. Its preparation method includes the following steps:

[0086] S1. Mix mannosyl erythritol ester with the repair oil composition, heat to dissolve into a transparent liquid and stir evenly at a stirring speed of 700 rpm for 8 min and a heating temperature of 75℃.

[0087] S2. Cool the dissolved oil phase from step S1 to 38°C;

[0088] S3. Heat water to 68°C, add the preservative composition, and dissolve and stir evenly at a stirring speed of 700 rpm for 25 minutes.

[0089] S4. When the aqueous phase stirred in step S3 is cooled to 38°C, add the soothing agent composition, moisturizing agent composition, serum albumin, and slender Geranium extract, dissolve and stir evenly at a stirring speed of 800 rpm for 25 min to obtain a transparent and homogeneous aqueous solution.

[0090] S5. Add the oil phase from step S2 to the aqueous phase from step S4 to obtain the shake-to-use essence emulsion. Shake before use until it becomes an opaque and uniform emulsion.

[0091] Comparative Example 5: (No serum albumin)

[0092] A shake-to-enriched essence, the composition and proportions of which are shown in Table 2, differs from Example 2 in that it contains no serum albumin. Its preparation method includes the following steps:

[0093] S1. Mix mannosyl erythritol ester with the repair oil composition, heat to dissolve into a transparent liquid and stir evenly at a stirring speed of 700 rpm for 8 min and a heating temperature of 75℃.

[0094] S2. Cool the dissolved oil phase from step S1 to 38°C;

[0095] S3. Heat water to 68°C, add the preservative composition, and dissolve and stir evenly at a stirring speed of 700 rpm for 25 minutes.

[0096] S4. When the aqueous phase stirred in step S3 is cooled to 38°C, add the soothing agent composition, moisturizing agent composition, recombinant humanized type IV collagen, and slender geranium extract, dissolve and stir evenly at 800 rpm for 25 min to obtain a transparent and homogeneous aqueous solution.

[0097] S5. Add the oil phase from step S2 to the aqueous phase from step S4 to obtain the shake-to-use essence emulsion. Shake before use until it becomes an opaque and uniform emulsion.

[0098] Comparative Example 6: (Geranium wilfordii extract without fine fibers)

[0099] A shake-to-enriched essence, the composition and proportions of which are shown in Table 2, differs from Example 2 in that it does not contain fine geranium extract. Its preparation method includes the following steps:

[0100] S1. Mix mannosyl erythritol ester with the repair oil composition, heat to dissolve into a transparent liquid and stir evenly at a stirring speed of 700 rpm for 8 min and a heating temperature of 75℃.

[0101] S2. Cool the dissolved oil phase from step S1 to 38°C;

[0102] S3. Heat water to 68°C, add the preservative composition, and dissolve and stir evenly at a stirring speed of 700 rpm for 25 minutes.

[0103] S4. When the aqueous phase stirred in step S3 is cooled to 38°C, add the soothing agent composition, moisturizing agent composition, recombinant humanized type IV collagen, and serum albumin. Dissolve and stir evenly at 800 rpm for 25 min to obtain a transparent and homogeneous aqueous solution.

[0104] S5. Add the oil phase from step S2 to the aqueous phase from step S4 to obtain the shake-to-use essence emulsion. Shake before use until it becomes an opaque and uniform emulsion.

[0105] Comparative Example 7: (Ratio of recombinant humanized type IV collagen to serum albumin 1:2)

[0106] A shake-to-enriched essence, the composition and ratio of its raw materials are shown in Table 2. The difference from Example 2 is that the mass ratio of recombinant humanized type IV collagen to serum albumin is 1:2.

[0107] The preparation method of the shake-to-enrich essence is the same as that in Example 1.

[0108] Comparative Example 8: (Ratio of recombinant humanized type IV collagen to serum albumin 2:1)

[0109] A shake-to-enriched essence, the composition and ratio of its raw materials are shown in Table 2. The difference from Example 2 is that the mass ratio of recombinant humanized type IV collagen to serum albumin is 2:1.

[0110] The preparation method of the shake-to-enrich essence is the same as that in Example 1.

[0111] Comparative Example 9: (without recombinant humanized type IV collagen, serum albumin, and Geranium slenderum extract)

[0112] A shake-to-enriched essence, the composition and proportions of which are shown in Table 2, differs from Example 2 in that it does not contain recombinant type IV humanized collagen, serum albumin, or Geranium fragrans extract. Its preparation method includes the following steps:

[0113] S1. Mix mannosyl erythritol ester with the repair oil composition, heat to dissolve into a transparent liquid and stir evenly at a stirring speed of 700 rpm for 8 min and a heating temperature of 75℃.

[0114] S2. Cool the dissolved oil phase from step S1 to 38°C;

[0115] S3. Heat water to 68°C, add the preservative composition, and dissolve and stir evenly at a stirring speed of 700 rpm for 25 minutes.

[0116] S4. When the aqueous phase stirred in step S3 is cooled to 38°C, add the soothing agent composition and the moisturizing agent composition, dissolve and stir evenly at a stirring speed of 800 rpm for 25 min to obtain a transparent and homogeneous aqueous solution.

[0117] S5. Add the oil phase from step S2 to the aqueous phase from step S4 to obtain the shake-to-use essence emulsion. Shake before use until it becomes an opaque and uniform emulsion.

[0118] Experimental Example 1: Comparison of layering speed, shaking state, and clarity after layering

[0119] Test samples: Shake-up essences prepared in Examples 1-3 and Comparative Examples 1-3.

[0120] Test method: Using a mixer, place the stirring paddle at 1 / 2 of the height of the shaken essence emulsion and stir at a constant speed of 200 rpm for 1 minute. Let it stand until it separates into layers. Measure the time required for the product to separate into layers from the point where stirring stops until there are no obvious oil droplets at the water-oil interface (i.e., separation time). Observe the shaking state of the product and the clarity of the water phase, oil phase, and oil-water interface after separation. The specific experimental results are shown in Table 3.

[0121] Table 3. Comparison of layering speed, shaking state, and layering clarity

[0122]

[0123] By comparing the layering speed, shaking state, and layering clarity of the shake-based essence emulsions of Examples 1-3 and Comparative Examples 1-3, it was found that the layering time of the shake-based essence emulsions of Examples 1-3 could reach 10-23 hours. After shaking, the water-oil dual phase could form a uniform emulsion under the instantaneous emulsification of mannosyl erythritol ester, and complete layering could be achieved within a certain time. Moreover, the water phase, oil phase, and oil-water interface were clear and transparent, providing the product with a good appearance and user experience. In contrast, the shake-up essence in Comparative Example 1 failed to achieve complete separation of the water and oil phases after shaking. Both the water and oil phases were in a blurry, semi-transparent state, and white streaks of emulsion appeared at the oil-water interface. This is presumably because sophorolipid is an anionic emulsifier with strong emulsifying ability, which affected the separation of water and oil. The shake-up essence in Comparative Example 2 contained PEG-6 caprylic / capric glyceride, which could achieve water-oil separation, but the oil phase was blurry after separation. The shake-up essence in Comparative Example 3 did not contain an emulsifier, so it could not form a uniform emulsion after shaking, and the separation was too rapid, affecting the uniformity of the sample.

[0124] In summary, in this invention, the water-oil essence containing mannitol erythritol ester can form a uniform emulsion after the water phase and oil phase are shaken together, and it does not separate into layers in a short time. This ensures that the water phase and oil phase can be evenly extracted when the product is used, and it automatically restores to the texture of a two-layer oil-water essence with a clear oil-water interface within a certain period of time, providing consumers with a richer skincare experience.

[0125] Experimental Example 2: Improvement of Sensitive Redness Test

[0126] Testing instrument: Visia-cr testing instrument.

[0127] Inclusion criteria for the test: volunteers aged 20-55 years, with a transdermal water loss (TEWL) ≥ 20 g / m² on the face before use. 2 •h, the questionnaire screened individuals as having sensitive skin; a total of 105 people met the above requirements, divided into 7 groups of 15 people each.

[0128] Test Method: Images of the subject's left, center, and right sides of the face were collected under three light sources: standard light 1, standard light 2, and cross-polarized light. Red area images derived from the cross-polarized light images were also collected. Image analysis was performed on the red area images to obtain the quantitative index, red area analysis a* value, which was used to assess the improvement of facial skin redness and sensitivity. The higher the red area analysis a* value, the more severe the skin redness.

[0129] The a* values ​​of the red area were analyzed in subjects using the samples from Example 2 and Comparative Examples 4-9 before use (D0), after 14 days of continuous use (D14), and on day 28 (D28), 14 days after discontinuation of product use. The test results are shown in Table 4. The a* value at D14 represents the improvement in red area on day 14 after product use, and the a* value at D28 represents the recurrence and sustained improvement of redness 14 days after discontinuation of product use. Images showing changes in red area in some subjects using the sample from Example 2 are shown below. Figure 3 As shown.

[0130] Calculation formula:

[0131] Initial value: refers to the baseline value when no samples are used.

[0132] average value: Where x = individual parameter measurement value of the subject, and n = number of valid data.

[0133]

[0134]

[0135] SPSS Statistics 25 was used for statistical analysis, with a two-tailed test and a significance level of α = 0.05. For continuous data, the difference analysis method was selected based on the normality test results: if the measurements were normally distributed, the t-test was used; if they were not normally distributed, the rank-sum test was used. Ordinal data were analyzed using the rank-sum test.

[0136] Table 4. Statistical results of a* values ​​in red zone analysis (n=15)

[0137]

[0138]

[0139] Note: Compared with the initial red zone analysis a* value, "-" indicates that the red zone analysis a* value has decreased; "*" indicates that the difference is statistically significant (0.01≤P<0.05); "**" indicates that the difference is statistically significant (0.001≤P<0.01).

[0140] Analysis of the red zone a* values ​​of the shake-up serums from Examples 2 and 4-9 showed that the short-term and long-term redness-reducing and stabilizing effects of the shake-up serum from Example 2 were significantly better than those of Comparative Examples 4-9. The changes in a* values ​​of Examples 2, Comparative Examples 4-6, and Comparative Example 9 on D14 and D28 revealed that the serum containing recombinant humanized type IV collagen, serum albumin, and Geranium extract exhibited superior redness-reducing effects compared to combinations containing only two of these ingredients or combinations without these ingredients. This demonstrates that recombinant humanized type IV collagen, serum albumin, and Geranium extract show a good synergistic effect in the long-term stabilization and repair of sensitive skin redness. Furthermore, the changes in a* values ​​of Examples 2 and 7-8 on D28 showed that recombinant humanized type IV collagen and serum albumin, at a specific mass ratio of 1:1, exhibited a good synergistic stabilizing effect.

[0141] In summary, this invention, by adding recombinant humanized type IV collagen, serum albumin, and Geranium wilfordii extract, and with the ratio of recombinant humanized type IV collagen to serum albumin being 1:1, exhibits a good synergistic repair effect on sensitive redness problems when combined with a soothing agent composition. It not only provides a good redness-reducing effect in the short term, but also provides long-term root-cause repair and maintenance of skin homeostasis after the product is discontinued, reducing the recurrence of sensitive redness problems.

Claims

1. A shakeable essence lotion with long-lasting effect in improving sensitive skin and redness, characterized in that, By weight percentage, it includes the following components: The product contains 5-25% repairing oils, 0.05-0.2% mannosyl erythritol ester, 0.05-0.1% recombinant humanized type IV collagen, 0.05-0.1% serum albumin, 1-5% geranium extract, 2-10% moisturizer, 1-5% soothing agent, 0.1-2% preservative, and water to 100%. The amino acid sequence of the recombinant humanized type IV collagen is shown in SEQ ID NO.1, and the serum albumin is plant-derived recombinant serum albumin.

2. The shake-to-enrich essence lotion as described in claim 1, characterized in that, The repairing oils include, but are not limited to, one or more of the following: squalane, meadowfoam seed oil, jojoba seed oil, camellia seed oil, olive oil, avocado oil, marula oil, burdock seed oil, coconut oil-caprylate / capric acid ester, and isononyl isononanoate.

3. The shake-to-enrich essence lotion as described in claim 2, characterized in that, The repairing oil is a composition of 1-5% squalane, 1-3% meadowfoam seed oil, 2-5% jojoba seed oil, and 5-10% coconut oil-caprylate / capric acid ester.

4. The shake-to-enrich essence lotion as described in claim 1, characterized in that, The mass ratio of the recombinant humanized type IV collagen to serum albumin is 1:

1.

5. The shake-to-enrich essence lotion as described in claim 1, characterized in that, The moisturizer includes, but is not limited to, one or more of the following: pentylene glycol, 1,3-butanediol, glycerin, xylitol glucoside, dehydrated xylitol, tremella fuciformis extract, β-glucan, D-panthenol, xylitol, sodium hyaluronate, and recombinant humanized type III collagen.

6. The shake-to-enrich essence lotion as described in claim 5, characterized in that, The moisturizer is a composition of 1,3-butanediol 1-3%, glycerin 1-3%, D-panthenol 0.5-2%, and sodium hyaluronate 0.05-0.1%.

7. The shake-to-enrich essence as described in claim 1, characterized in that, The soothing agent is DEFENSIL. ® -SOFT, SymSitive ® 1609. One or more of the following components: oat kernel extract, bisabolol, dipotassium glycyrrhizate, and horse chestnut extract.

8. The shake-to-enrich essence lotion as described in claim 7, characterized in that, The soothing agent is DEFENSIL. ® A composition of 1.0-3.0% SOFT, 0.1-0.2% dipotassium glycyrrhizate, and 0.2-1% Aesculus chinensis extract.

9. The shake-to-enrich essence as described in claim 1, characterized in that, The preservative includes a combination of one or more of the following: p-hydroxyacetophenone, hexanediol, ethylhexylglycerin, and caprylyl glycol.

10. The shake-to-enrich essence as described in claim 9, characterized in that, The preservative is a composition of 0.3-0.5% hexanediol and 0.3-0.5% p-hydroxyacetophenone.

11. The method for preparing the shake-to-apply serum with long-lasting effect in improving sensitive skin and redness as described in claim 1, characterized in that, Includes the following steps: S1. Mix mannosyl erythritol with repair oil, heat to dissolve into a transparent liquid and stir evenly. The heating temperature is 70~80℃. S2. Cool the dissolved oil phase from step S1 to 35~42℃; S3. Heat the water to 65~70℃, add the preservative, and stir until dissolved and evenly mixed; S4. When the aqueous phase stirred in step S3 is cooled to 35~42℃, add soothing agent, moisturizer, recombinant humanized type IV collagen, serum albumin, and geranium extract, dissolve and stir evenly to obtain a transparent and homogeneous aqueous solution. S5. Add the oil phase from step S2 to the aqueous phase solution from step S4 to form the shake-to-use essence emulsion. Shake before use until it becomes an opaque and uniform emulsion.

12. The preparation method according to claim 11, characterized in that, The stirring conditions described in step S1 are: stirring speed 600~800 rpm, stirring time 5~10 min.

13. The preparation method according to claim 11, characterized in that, The stirring conditions described in step S3 are: stirring speed 600~800 rpm, stirring time 20~30 min.

14. The preparation method according to claim 11, characterized in that, The stirring conditions described in step S4 are: stirring speed 600~1000 rpm, stirring time 20~30 min.

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