A coating agent for isolating microorganisms and fertilizers and its application

Abstract

The invention discloses a coating agent for blocking microorganisms and fertilizers and application thereof. The coating agent comprises low-melting-point wax powder, woodlice powder, rice husk powder and corn stalk powder. The coating agent is coated on the surface of the fertilizer, and then a microbial oil agent is sprayed to obtain a microbial coated fertilizer. The coating agent makes the coated fertilizer and the microorganisms have good compatibility, and various microbial fertilizer products such as microbial compound fertilizer and microbial organic fertilizer can be prepared, and the microbial spores still maintain high activity. In addition, after applying the microbial coated fertilizer of the invention, the crop yield can be increased, and the disease resistance of the crop can be improved. The process is simple and the cost is low.

Description

Technical Field

[0001] The invention relates to the technical field of pesticides, and in particular to a packaging agent for isolating microorganisms and fertilizers and application thereof. Background Art

[0002] In recent years, the extensive use of chemical fertilizers has not only polluted the environment, but also caused ecological deterioration, soil hardening and other problems, while reducing the quality of agricultural products. In order to promote the sustainable development of agriculture, countries around the world are constantly working to find the best solution. Microbial fertilizers can not only increase soil fertility and reduce the use of chemical fertilizers, but also purify and repair the soil, playing an important role in improving quality and yield, reducing the occurrence of plant diseases, and improving food safety.

[0003] Microbial fertilizers are divided into bacterial fertilizers (rhizobium fertilizers, nitrogen-fixing bacteria fertilizers), actinomycete fertilizers (such as antibiotics), fungal fertilizers (such as mycorrhizal fungi), etc. according to the specific types of microorganisms in their products. Microbial fertilizers are of many types and complete functions, mainly involving biocontrol microorganisms, such as insecticidal microorganisms Metarhizium and Beauveria bassiana, fungicidal and growth-promoting microorganisms Trichoderma, and fungicidal microorganisms Bacillus subtilis. These microorganisms have a broad spectrum of effects and often have multiple functions. For example, because they can form hyphae in the soil, they can regulate soil biological populations and improve soil structure. The insecticidal microorganism Metarhizium CQMa421 also has disease resistance and growth-promoting effects. Trichoderma and Bacillus subtilis have good antibacterial effects on more than 10 plant pathogens.

[0004] Despite this, there are still relatively few microbial fertilizers and their stability is poor. The reason is that living microorganisms are sensitive to the environment, such as high-salt environment, high-osmotic environment, etc., and the ingredients in fertilizers are complex, often containing chloride and nitrate ions and high salt or acidic or alkaline, and most fertilizers contain miscellaneous bacteria, all of which have an inhibitory effect on microbial activity, greatly limiting the application of microbial fertilizers.

[0005] By using wrapping materials to block direct contact between fertilizers and microorganisms, that is, isolating microorganisms from the high-salt environment of fertilizers, it is expected to prepare stable microbial fertilizers. At present, the packaging technology of fertilizers is mainly liquid spray or stirring, which is used to prevent caking, dusting, moisture absorption, phase change and crystallization of granular fertilizers. There are many types of these fertilizer wrapping agents, and the wrapped fertilizers are difficult to be compatible with microorganisms for a long time. They are not suitable for the packaging processing of microbial fertilizers, which affects the stability of the application effect of bacterial fertilizers. The present invention proposes a wrapping material that blocks microorganisms and fertilizers. The material is unique, low-cost, and easy to operate. It greatly improves the compatibility of various microorganisms with different fertilizers, and is expected to promote the development of microbial fertilizers.

[0006] CN105948915A discloses a fertilizer synergistic coating agent, which is made of a ratio of water-soluble organic matter and microbial agents. The water-soluble organic matter is selected from the substance formed by drying all the water-soluble fermentation liquid produced in the process of preparing amino acids and / or ethanol. The invention improves the coating rate and contains a protective agent, which can improve the survival rate of microorganisms and increase the number of effective live bacteria in the soil within a certain period of time, and can improve the soil condition. However, the invention uses a coating machine for coating, and the coating machine has a high temperature, which will kill a large number of microorganisms. The invention does not detect the activity of microorganisms on the coated fertilizer, but only determines the number of microorganisms in the soil after fertilization. Adding fermented organic matter will increase the number of microorganisms in the soil.

[0007] CN201711165601.8 discloses a preparation method for coated slow-release fertilizer, wherein the coating material is rice husk. The rice husk of the invention is activated carbon powder after high-temperature carbonization, which has high cost and the cellulose structure of the rice husk is destroyed. The function of the fertilizer is to have strong adhesion and reduce agricultural pollution caused by organic fertilizer. Summary of the invention

[0008] In view of this, one of the objects of the present invention is to provide a packaging agent for blocking microorganisms and fertilizers, including packaging material 1 and packaging material 2, wherein packaging material 1 is low-melting point wax powder, and packaging material 2 is a combination of woodlice powder, rice husk powder and corn stalk powder.

[0009] Preferably, the wrapping agent is composed of the wrapping material 1 and the wrapping material 2, the weight ratio of the wrapping material 1 to the wrapping material 2 is 1:(3-5), and the mass fractions of woodlice powder, rice husk powder and corn stalk powder in the wrapping material 2 are 60%, 10-30% and 10-30% respectively.

[0010] The second object of the present invention is to provide a microbial coated fertilizer, comprising a microorganism, a coating agent and a chemical fertilizer or a compound fertilizer, wherein the microorganism comprises one or more of Metarhizium anisopliae, Beauveria bassiana, Trichoderma harzianum and Bacillus subtilis, the coating agent comprises a coating material 1 and a coating material 2, the coating material 1 is a low melting point wax powder, the coating material 2 is a combination of pillbug powder, rice husk powder and corn stalk powder, the coating agent is coated on the outer surface of the chemical fertilizer or the compound fertilizer, and the microorganism is attached to the surface of the coating agent or / and in the coating agent.

[0011] Preferably, the wrapping agent is composed of the wrapping material 1 and the wrapping material 2, the weight ratio of the wrapping material 1 to the wrapping material 2 is 1:(3-5), and the mass fractions of woodlice powder, rice husk powder and corn stalk powder in the wrapping material 2 are 60%, 10-30% and 10-30% respectively.

[0012] Preferably, the mass fractions of woodlice powder, rice husk powder and corn stalk powder in the wrapping material 2 are 60%, 10% and 30%, or 60%, 30% and 10%, or 60%, 20% and 20%, respectively.

[0013] Preferably, the fertilizer is calcium ammonium nitrate fertilizer with a particle size of 1-15 mm; the compound fertilizer is an eight-in-one compound fertilizer with a particle size of 1-15 mm.

[0014] Preferably, the weight ratio of the wrapping material 1 to the chemical fertilizer or compound fertilizer is 1:100, and the weight ratio of the wrapping material 2 to the chemical fertilizer or compound fertilizer is (3-5):100.

[0015] Preferably, the amount of the microorganism is 1×10 7 spore.

[0016] The third object of the present invention is to provide a method for preparing the above-mentioned microbial coated fertilizer, comprising the following steps: adding the coating agent to the chemical fertilizer or compound fertilizer, and then rotating it in an 80°C oven for 5-10 minutes to evenly coat the coating agent on the surface of the chemical fertilizer or compound fertilizer, and then cooling it to room temperature, evenly spraying the microbial oil agent on the surface of the coated fertilizer to obtain the microbial coated fertilizer.

[0017] A fourth object of the present invention is to provide the use of the above-mentioned encapsulating agent in isolating microorganisms from chemical fertilizers or compound fertilizers.

[0018] A fifth object of the present invention is to provide the use of the above-mentioned microorganism-encapsulated fertilizer in preventing and controlling crop diseases and insect pests and / or promoting crop growth.

[0019] The coating agent of the invention comprises low melting point wax powder, woodlice powder, rice husk powder and corn stalk powder. The coating agent is coated on the surface of fertilizer (chemical fertilizer or compound fertilizer), and then a microbial oil agent is sprayed to obtain microbial coated fertilizer. The coating agent of the invention makes the coated fertilizer and microorganisms have good compatibility, and various microbial fertilizer products such as microbial compound fertilizer and microbial organic fertilizer can be prepared, and the microbial spores still maintain high activity. In addition, the application of the microbial coated fertilizer of the invention can increase crop yield and improve the disease resistance of crops, and the process is simple and the cost is low. DETAILED DESCRIPTION

[0020] The present invention will be described in detail below in conjunction with examples, which are merely illustrative and are not intended to limit the scope of application of the present invention. The present invention is not limited to the following embodiments or examples, and any modifications and variations that do not violate the spirit of the present invention should be included within the scope of the present invention.

[0021] Experimental materials and sources

[0022] 1. Test strains

[0023] 15 billion spores / g of Metarhizium anisopliae CQMa421 spore powder (Chongqing Julixin Bioengineering Co., Ltd.)

[0024] 80 billion spores / gram Beauveria bassiana ZJU435 spore powder (Chongqing Julixin Bioengineering Co., Ltd.)

[0025] 30 billion spores / Trichoderma krazii XZN213-1 spore powder (Chongqing Julixin Bioengineering Co., Ltd.)

[0026] 100 billion spores / g Bacillus subtilis spore powder (Shandong Rushan Hanwei Biotechnology Co., Ltd.) 2. Test fertilizer (fertilizer particle size 1-15mm)

[0027] Compound fertilizer: eight-in-one compound fertilizer (total nutrient N-P2O5-K2O=29-9-11≥40%, organic long-acting ingredients≥8%) (Chongqing Wanzhi Jufeng Ecological Fertilizer Co., Ltd.)

[0028] Fertilizer: Calcium ammonium nitrate fertilizer (nitrogen content ≥ 15%, of which nitrate nitrogen ≥ 14%, Ca ≥ 18%) (Henan Zhenying Biotechnology Co., Ltd.)

[0029] 3. Main reagents

[0030] Low melting point wax powder (Shanghai Joule Wax Co., Ltd.), rice husk powder (Mianyang Zaiweibao Technology Co., Ltd.), corn stalk powder (Shanyang Lianfeng Biotechnology Co., Ltd.), rape stalk powder (Gene Engineering Center of Chongqing University), woodlice powder (Shaanxi Quanao Biotechnology Co., Ltd.), earthworm powder (Shandong Huiheng Biotechnology Co., Ltd.), silkworm pupa powder (Shaanxi Quanao Biotechnology Co., Ltd.), these seven powders are used for related experiments on the exploration of packaging material components and composition, and all need to be crushed to 200 mesh before being used in the following experiments; soybean oil (Hubei Chuxing Grain and Oil Co., Ltd.), sterilized 1 / 4SDAY medium, sterilized PDA medium, sterilized LB liquid / solid medium, sterilized water agar medium, sterile water, 0.1% Tween-80 solution.

[0031] Experimental Example 1 Determination of compatibility of seven powders with microorganisms

[0032] Method: Low melting point wax powder, rice husk powder, corn stalk powder, rape stalk powder, pill bug powder, earthworm powder, silkworm pupa powder were mixed with green anisopliae CQMa421, Beauveria bassiana ZJU435, Trichoderma harzianum XZN213-1 or Bacillus subtilis spore powder at a mass ratio of 100:1, and stored in a 37°C incubator for 14 days. The spore germination rate was tested to detect whether the above seven powders had an effect on the germination of various microbial spores. Spore germination rate detection method: 1g of each combination mixture was diluted to 1×10 using 0.1% Tween-80 solution. 6 spores / mL bacterial solution, 100 μL of bacterial solution was evenly spread on a 1 / 4SDAY plate, and cultured at 26°C for 14-16 h before counting the germination rate (Bacillus subtilis was diluted to 1×10 3 Spores / mL bacterial solution, 100 μL bacterial solution was evenly spread on the LB plate, and CFU was determined after culturing at 37°C for 14-16 hours). Pure spores were used as the control, and each treatment was repeated 3 times, that is, each treatment sample after being stored at 37°C for 14 days was taken out 3 times, bacterial solution was prepared 3 times, and the determination was performed 3 times.

[0033] Results: Table 1 shows that after each mixture was cultured at 37°C for 14 days, the germination rates of the spores of Metarhizium CQMa421, Beauveria ZJU435 and Trichoderma harzianum XZN213-1 mixed with low melting point wax powder, rice husk powder, corn stalk powder, rape stalk powder, pillbug powder and earthworm powder were 60%-68% and the CFU of Bacillus subtilis was 54-60, which were comparable to the control and had no negative impact on spore germination. They were significantly higher than the germination rate of 46-53% and CFU45 of the microbial spores mixed with silkworm pupa powder. Therefore, low melting point wax powder, rice husk powder, corn stalk powder, rape stalk powder, pillbug powder and earthworm powder have good compatibility with microorganisms and can be used as fertilizer packaging materials.

[0034] Table 1 Effects of seven powders on the germination rate of microbial spores (37°C)

[0035]

[0036] Experimental Example 2 Screening of raw materials conducive to microbial growth

[0037] Method: According to the requirements in Table 2, sterilized water agar plates of equal thickness, PDA plates, and agar plates containing 2% powder (prepared at a weight ratio of powder to water agar of 2:100, and the powder was added after the water agar was sterilized, and the powder was not sterilized) were prepared respectively. PDA plates on which Metarhizium anisopliae CQMa421, Beauveria bassiana ZJU435, and Trichoderma harzianum XZN213-1 were grown for 10 days were punched with a 9mm hole puncher, and 9mm diameter Metarhizium anisopliae CQMa421, The fungus cakes of Beauveria bassiana ZJU435 and Trichoderma XZN213-1 were inoculated on agar plates and the center of PDA plates with different 2% powders and cultured at 27±1°C for 10 days. The growth of Green Anisopliae CQMa421, Beauveria bassiana ZJU435 and Trichoderma XZN213-1 on the plates was observed and the colony size was measured. Water agar plates were used as controls. Each treatment was repeated three times to calculate the relative growth rate of the colonies. Relative growth rate (%) = 100*(colony diameter - 9 mm) / 9 mm. According to the requirements in Table 2, prepare equal volumes of LB liquid medium, sterile water, liquid medium containing 2% powder (prepared in a weight ratio of powder to sterile water of 2:100), and 1 bottle of Bacillus subtilis liquid (obtained by shaking culture for 12-16 h at 36 ± 1 ° C, 200 r / min after inoculation of Bacillus subtilis in LB liquid medium), inoculate 1 mL of Bacillus subtilis liquid in each culture medium, use sterile water as a control, shake culture for 24 h at 36 ± 1 ° C, 200 r / min, and each treatment is repeated three times. The concentration of Bacillus subtilis in the culture medium before and after shaking culture is determined by the method of gradient dilution coating plates, and the growth multiple of Bacillus subtilis before shaking culture is calculated after each culture medium is shaken, and the growth multiple=100*(the number of Bacillus subtilis after shaking culture-the number of Bacillus subtilis before shaking culture) / the number of Bacillus subtilis before shaking culture.

[0038] Results: As shown in Table 2, the treatments with 2% pillbug powder, 2% earthworm powder, and 2% silkworm pupa powder respectively promoted the growth of Metarhizium anisopliae CQMa421, Beauveria bassiana ZJU435, and Trichoderma XZN213-1, but had no obvious growth-promoting effect on Bacillus subtilis. The treatments with 2% rice husk powder, 2% corn stalk powder, and 2% rapeseed stalk powder respectively promoted the growth of Bacillus subtilis, but had no obvious growth-promoting effect on Metarhizium anisopliae CQMa421, Beauveria bassiana ZJU435, and Trichoderma XZN213-1. Therefore, we selected woodlice powder, rice husk powder and corn stalk powder, which promote the growth of Metarhizium anisopliae CQMa421, Beauveria bassiana ZJU435, Trichoderma XZN213-1 or Bacillus subtilis, for subsequent exploratory experiments on the combination of encapsulating agents. Low-melting-point wax powder has no negative impact on the germination of microbial spores and has an adhesion effect (it is expected to be used as a medium for adhering fertilizers). Therefore, low-melting-point wax powder is also used as one of the encapsulating agent ingredients in subsequent experiments.

[0039] Table 2 Screening of raw materials that are beneficial to microbial growth

[0040]

[0041]

[0042] Experimental Example 3 Encapsulation Method and Encapsulation Agent Combination Screening

[0043] Preparation 10 9 spores / mL of the oil preparation of Metarhizium anisopliae CQMa421 (the spore powder was diluted 15 times with soybean oil), the oil preparation of Beauveria bassiana ZJU435 (the spore powder was diluted 80 times with soybean oil), the oil preparation of Trichoderma harzianum XZN213-1 (the spore powder was diluted 30 times with soybean oil) and the oil preparation of Bacillus subtilis (the spore powder was diluted 100 times with soybean oil).

[0044] The components listed in Table 3 are combined to prepare a wrapping agent (the wrapping agent consists of wrapping material 1 and wrapping material 2, wrapping material 1 is low melting point wax powder; wrapping material 2 consists of at least one of woodlice powder, rice husk powder and corn stalk powder. The specific composition is shown in the 13 combinations shown in Table 3). Preparation method of encapsulating agent and microbial encapsulated fertilizer: low melting point wax powder and encapsulating material 2 (encapsulating material 2 has 13 combinations) are mixed uniformly at a weight ratio of 1:3 to obtain 13 combinations of encapsulating agents (i.e., formula 1 to formula 13 in Table 3), the encapsulating agent is added to granular fertilizer or compound fertilizer (particle size 1-15 mm), and is placed in an 80°C oven and rotated continuously for 5-10 minutes to uniformly encapsulate the fertilizer surface with the encapsulating agent to obtain encapsulated fertilizer, and then cooled to room temperature, and then the surface of the encapsulated fertilizer is sprayed with Metarhizium anisopliae CQMa421, Beauveria bassiana ZJU435, Trichoderma harzianum XZN213-1 or Bacillus subtilis oil agent while rotating and shaking using a spray tower to obtain microbial encapsulated fertilizer. In the microbial encapsulation materials prepared in this experimental example, the weight ratio of encapsulation material 1, i.e., low-melting-point wax powder, to fertilizer (i.e., chemical fertilizer or compound fertilizer) is 1:100, recorded as 1% low-melting-point wax powder; the weight ratio of encapsulation material 2 to fertilizer (i.e., chemical fertilizer or compound fertilizer) is 3:100, recorded as 3% encapsulation material 2; the amount of microbial oil agent is 1 mL of oil agent sprayed per 100 g of fertilizer (i.e., chemical fertilizer or compound fertilizer), even if each gram of chemical fertilizer or compound fertilizer contains 1×10 7 spore.

[0045] The prepared microorganism-encapsulated fertilizer was sealed and stored at 37°C for 10 days, and then the spore germination rate was tested: the microorganism-encapsulated fertilizer was diluted with 0.1% Tween-80 solution to 1×10 6spores / mL bacterial solution, and then 100 μL of bacterial solution was evenly spread on a 1 / 4SDAY plate and cultured at 26°C for 14-16 hours before counting the germination rate (Bacillus subtilis coated fertilizer was diluted with 0.1% Tween-80 solution to 1×10 3 spores / mL bacterial solution, 100 μL of bacterial solution was evenly spread on the LB plate and cultured at 37°C for 14-16 h before determining CFU), and unwrapped fertilizer (i.e., chemical fertilizer or compound fertilizer directly sprayed with microbial oil) was used as the control. Each treatment was repeated 3 times, that is, each treatment sample that was sealed and stored for 10 days was taken out 3 times, bacterial solution was prepared 3 times, and determination was performed 3 times.

[0046] Table 3 Effect of encapsulated fertilizer on germination of microbial spores

[0047]

[0048]

[0049] From the data in Table 3, it can be seen that after the compound fertilizer or chemical fertilizer was wrapped with only 1% low melting point wax powder and 3% wrapping material 2, the activity of the four microbial spores was significantly higher than that of the control. Among them, the comprehensive protection effect of formula 9-11 on microorganisms after the fertilizer was wrapped with the wrapping agent was significantly better than that of other formulas. Therefore, formula 9-11 was selected for the subsequent wrapping agent dosage experiment.

[0050] Experimental Example 4 Effect of different dosages of encapsulating agent on encapsulation effect

[0051] The encapsulating agent of formula 9-11 in Experimental Example 3 was selected for this experiment, and the fertilizer (chemical fertilizer or compound fertilizer) was encapsulated according to the dosage in Table 4: dosage 1 means that the weight ratio of the encapsulating material 2 to the fertilizer in the encapsulating agent is 1:100, recorded as 1% of the encapsulating material 2; dosage 3 means that the weight ratio of the encapsulating material 2 to the fertilizer is 3:100, recorded as 3% of the encapsulating material 2; dosages 5, 7, and 9 respectively mean that the weight ratio of the encapsulating material 2 to the fertilizer is 5:100, 7:100, and 9:100. :100, 9:100, recorded as 5%, 7%, 9% wrapping material 2; dosage 0, indicating that no wrapping agent is used; the dosage of wrapping material 1, i.e., low melting point wax powder, in the wrapping agent is 1:100 in weight ratio with the fertilizer (i.e., chemical fertilizer or compound fertilizer), recorded as 1% low melting point wax powder), the wrapping method, microorganism dosage, spore germination rate detection, etc. are all referenced to Example 3, with unwrapped fertilizer (i.e., only fertilizer sprayed with microorganisms) as the control, and each treatment is repeated 3 times.

[0052] Table 4 Effect of fertilizer packaging on spore germination of four microorganisms

[0053]

[0054] It can be seen from the data in Table 4 that, compared with the control, the activity of microbial spores was significantly improved after the compound fertilizer or chemical fertilizer was wrapped with the wrapping agent at a dosage of 1-9% of the wrapping material 2, among which the dosage of 3-5% had the best comprehensive effect and low cost.

[0055] Experimental Example 5: Long-term storage effect and field application effect of fertilizer wrapped with encapsulating agent

[0056] 1. Package compound fertilizer

[0057] The microbial coated fertilizer was prepared by coating the compound fertilizer with the coating agent of the formula 11 in Table 3 above and the weight ratio of 1% low melting point wax powder and 3% coating material 2, and the germination rate was tested after sealing and storing at room temperature (15-35° C.) for 6 months (see Experimental Example 3 for the coating method, microbial dosage, spore germination rate detection method, etc.).

[0058] Table 5 Determination of storage activity of microorganisms + compound fertilizers

[0059] product Microbial spore germination rate or CFU <![CDATA[10 7 Spores / g of Metarhizium compound fertilizer]]> 52％ <![CDATA[10 7 Spores / g of Metarhizium compound fertilizer (unwrapped)]]> 0 <![CDATA[10 7 Spores / g of Beauveria bassiana compound fertilizer]]> 41％ <![CDATA[10 7 Spores / g of Beauveria bassiana compound fertilizer (unwrapped)]]> 0 <![CDATA[10 7 Spores / g of Trichoderma harzianum compound fertilizer]]> 64％ <![CDATA[10 7 Spores / g of Trichoderma harzianum compound fertilizer (unwrapped)]]> 0 <![CDATA[10 7 Spores / g of Bacillus subtilis compound fertilizer]]> 43 <![CDATA[10 7 Spores / g of Bacillus subtilis compound fertilizer (unwrapped)]]> 2

[0060] 2. Package fertilizer

[0061] The fertilizer was packaged with the packaging agent of the formula 11 in Table 3 above and 1% low melting point wax powder and 3% packaging material 2 in a weight ratio to prepare a microbial packaged fertilizer. The fertilizer was sealed and stored at room temperature (15-35° C.) for 6 months, and the germination rate was tested (see Experimental Example 3 for the packaging method, microbial dosage, spore germination rate detection method, etc.).

[0062] Table 6 Storage activity determination of microorganisms + fertilizers

[0063]

[0064]

[0065] 3. Field experiment

[0066] The microbial encapsulated fertilizers of Trichoderma harzianum fertilizer and Bacillus subtilis fertilizer products in Table 6 were selected to test cabbage. The test site was Chongqing Yuxi Vegetable Research and Development Center. The seeds were sown in August 2023. There were 5 treatments, 3 replicates for each treatment, and an area of ​​1 mu for each treatment. The fertilizer application rate was 40 kg / mu, and other management measures were the same. About 50 days after fertilization, mature cabbage was harvested, and the cabbage yield and incidence rate of each treatment were measured and compared. The incidence rate was calculated as follows: the weight of cabbage with lesions in each treatment / the total amount of cabbage harvested in each treatment * 100%.

[0067] Table 7 Growth-promoting and disease-resistant effects of microorganisms + fertilizers

[0068]

[0069] As shown in the data of Tables 5-7, the microbial compound fertilizer or fertilizer product microbial coated fertilizer prepared by using the coating agent of the present invention, after storage for 6 months, still maintains a higher activity of the microorganisms compared with the control, and after application, can significantly improve the yield and disease resistance of cabbage, and is worthy of promotion and application.

[0070] It should be noted that some of the experimental operations involved in the above experimental examples have certain universality and are therefore not described in detail. For some contents that are not described in detail, reference is made to related operations in other experimental examples or to the prior art and will not be repeated here.

Claims

1. A packaging agent for isolating microorganisms and fertilizers, characterized in that: The invention comprises a wrapping material 1 and a wrapping material 2, wherein the wrapping material 1 is low melting point wax powder, and the wrapping material 2 is a combination of woodlice powder, rice husk powder and corn stalk powder; the weight ratio of the wrapping material 1 to the wrapping material 2 is 1:(3-5), and the mass fractions of the woodlice powder, rice husk powder and corn stalk powder in the wrapping material 2 are 60%, 10-30% and 10-30% respectively.

2. The encapsulation agent according to claim 1, characterized in that The wrapping agent consists of the wrapping material 1 and the wrapping material 2.

3. A microorganism-encapsulated fertilizer, characterized in that: The invention comprises a microorganism, a wrapping agent and a fertilizer or a compound fertilizer, wherein the microorganism comprises one or more of Metarhizium anisopliae, Beauveria bassiana, Trichoderma harzianum and Bacillus subtilis, the wrapping agent comprises a wrapping material 1 and a wrapping material 2, the wrapping material 1 is a low melting point wax powder, the wrapping material 2 is a combination of woodlice powder, rice husk powder and corn stalk powder, the wrapping agent is wrapped on the outer surface of the fertilizer or compound fertilizer, and the microorganism is attached to the surface of the wrapping agent or / and in the wrapping agent; the weight ratio of the wrapping material 1 to the wrapping material 2 is 1:(3-5), the weight fractions of woodlice powder, rice husk powder and corn stalk powder in the wrapping material 2 are 60%, 10-30% and 10-30% respectively; the weight ratio of the wrapping material 1 to the fertilizer or compound fertilizer is 1:100, and the weight ratio of the wrapping material 2 to the fertilizer or compound fertilizer is (3-5):100; the amount of the microorganism is 1×10 7 spores; the fertilizer is calcium ammonium nitrate fertilizer; the compound fertilizer is an eight-in-one compound fertilizer.

4. The microorganism-encapsulated fertilizer according to claim 3, characterized in that: The wrapping agent consists of the wrapping material 1 and the wrapping material 2.

5. The method for preparing the microorganism-encapsulated fertilizer according to claim 3 or 4, characterized in that: The method comprises the following steps: adding the encapsulating agent to the chemical fertilizer or compound fertilizer, and then rotating the encapsulating agent in an oven at 80°C for 5-10 minutes to evenly encapsulate the surface of the chemical fertilizer or compound fertilizer, and then evenly spraying the microbial oil agent on the surface of the encapsulated fertilizer after cooling to room temperature to obtain microbial encapsulated fertilizer.

6. Use of the encapsulating agent as claimed in claim 1 or 2 in isolating microorganisms from chemical fertilizers or compound fertilizers.

7. Use of the microorganism-encapsulated fertilizer as claimed in claim 3 or 4 in preventing and controlling crop diseases and insect pests and / or promoting crop growth.

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