A method for artificial breeding of hybrid tiger dragon spot

Abstract

The invention provides an artificial seedling method of a tiger-dragon hybrid spot, and relates to the field of aquaculture. An artificial seedling method of a tiger-dragon hybrid spot comprises the following steps: (1) selecting an outdoor high-level pond, selecting tiger-dragon hybrid spot fry over 7 days old, and putting the tiger-dragon hybrid spot fry into the high-level pond; (2) feeding rotifers from 7 days to 14 days old, and inoculating protein nucleus Chlorella liquid and halogenated Dunaliella liquid in the pond at the same time; feeding Artemia and Mongolian monilopsis from 15 days to 30 days old; feeding Mongolian monilopsis and yellow mealworm from 31 days to 50 days old; (3) when encountering a rainstorm, feeding a nutrient within 24 hours after the rain. The invention has a high survival rate of seedlings, a large size of the obtained fry, a good seedling effect, and a low mutation rate, and better guarantees the seedling quality of the tiger-dragon hybrid spot. The obtained tiger-dragon hybrid spot fry has stronger resistance, and the subsequent breeding can better cope with the adverse weather effects, which can effectively reduce the breeding risk of the tiger-dragon hybrid spot and significantly improve the breeding efficiency of the tiger-dragon hybrid spot.

Description

Technical Field

[0001] The invention relates to the field of aquaculture, and in particular to an artificial seedling breeding method for tiger dragon hybrid grouper. Background Art

[0002] The hybrid grouper is a fish that was bred by two generations of groups, with a female brown-spotted grouper as the mother and a male saddle-banded grouper as the father. The hybrid grouper is the F1 generation, which is the hybrid grouper. The most obvious characteristics of the hybrid grouper are fast growth, smaller coefficient of variation of body length and weight, significantly improved uniformity, and relatively stable inheritance of traits. After 8 years of breeding, it was approved as a new aquatic product by the state in 2017. The hybrid grouper grows fast, with an average weight gain 114.52% faster than the mother, and the breeding cycle is shortened by more than half.

[0003] The breeding of tiger dragon hybrid spot is mainly indoors, which requires light every day and has a relatively high breeding cost. Outdoor breeding often has a low survival rate and a high mutation rate, which reduces the yield of fry. Summary of the invention

[0004] In view of this, the present invention proposes an artificial breeding method for tiger dragon hybrid grouper, under outdoor breeding conditions, the breeding survival rate is high, the mutation rate is low, and the fry size is large.

[0005] The technical solution of the present invention is achieved in this way:

[0006] An artificial seedling raising method of tiger dragon hybrid spot comprises the following steps:

[0007] (1) selecting an outdoor high-position pond, selecting tiger-dragon hybrid spotted fish fry over 7 days old, and placing the tiger-dragon hybrid spotted fish fry into the high-position pond;

[0008] (2) Rotifers were fed to the 7- to 14-day-olds, and the pool was inoculated with Chlorella pyrenoidosa and Dunaliella salina liquid;

[0009] From 15 to 30 days old, feed Artemia and Mongoloid fleas;

[0010] From 31 to 50 days old, they were fed with Moina mongolica and mealworms;

[0011] (3) When encountering heavy rain, feed nutrients within 24 hours after the rain.

[0012] A further technical solution is that the area of ​​the high-level pool is 1000-3000m 2 , the pool depth is 3-4m and the water level is 2.5-3.5m.

[0013] A further technical solution is that in step (1), the stocking density of fry is 0.6-0.8 thousand fry / m 3 .

[0014] A further technical solution is that from 7 days to 14 days old, the rotifer density is maintained at 5-10 / mL.

[0015] A further technical solution is that the rotifers are composed of SS-type rotifers and L-type rotifers, and the number ratio of SS-type rotifers to L-type rotifers is 1:2-4.

[0016] A further technical solution is that the inoculation amount of the pyrenoid chlorella liquid is 5-10mL / m 2 The inoculation volume of Dunaliella salina liquid is 2-4mL / m 2 ;

[0017] The cell density of Chlorella pyrenoidosa solution is 25-30 billion cells / ml, and the cell density of Dunaliella salina solution is 15-20 billion cells / ml.

[0018] A further technical solution is that from 15 days to 30 days old, the density of Artemia is maintained at 10-15 pieces / mL, and the density of Mongoloid flea is maintained at 3-5 pieces / mL.

[0019] A further technical solution is that from 31 days to 50 days old, the density of Moina mongolica is maintained at 15-20 / mL, and the daily feeding amount of yellow mealworms is 2%-3% of the total weight of the fry.

[0020] A further technical solution is that the nutrient agent is prepared by comprising the following raw materials in parts by weight: 10-20 g of Haematococcus pluvialis powder, 1-2 g of nicotinamide, 3-5 g of thiamine, 1-2 g of astaxanthin, 10-20 g of disodium EDTA, 100-200 g of ammonium sulfate, 30-40 g of superphosphate and 0.9-1.1 kg of water.

[0021] A further technical solution is that in step (3), the amount of the nutrient added is 1-2 mg / m 2 .

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) The present invention is directed to outdoor high-level ponds for breeding of hybrid tiger-dragon spot. In the first stage, a certain ratio of rotifers is fed and a pyrenoid Chlorella solution and a halogenated Dunaliella solution are inoculated. In the second stage, an appropriate amount of Artemia and Mongolica are fed. In the third stage, an appropriate amount of Mongolica and Tenebrio molitor are fed. After the rain, a special nutrient is fed. The breeding survival rate of the seedlings is high, the fry size is large, the breeding effect is better, and the mutation rate is low, thereby better ensuring the breeding quality of the hybrid tiger-dragon spot.

[0024] (2) The seedling raising method of the present invention can achieve good seedling raising effect under outdoor seedling raising conditions, and the seedling raising cost is relatively low. In addition, the obtained tiger-dragon hybrid grouper fry has stronger resistance, and subsequent breeding can better cope with adverse weather effects, which can effectively reduce the risk of tiger-dragon hybrid grouper breeding and significantly improve the efficiency of tiger-dragon hybrid grouper breeding.

[0025] (3) The present invention adopts a high-level pond to raise seedlings of tiger dragon hybrid spot, which not only increases the amount of seedlings put in and increases the scale of seedling raising, but also the outdoor high-level pond better simulates the ocean space, effectively promoting the growth and development of tiger dragon hybrid spot. DETAILED DESCRIPTION

[0026] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0027] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0028] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0029] The Chlorella pyrenoidosa liquid used in the embodiment of the present invention is a Chlorella pyrenoidosa concentrated liquid, and its cell density is 25-30 billion cells / ml.

[0030] The Dunaliella salina liquid used in the embodiment of the present invention is a concentrated liquid of Dunaliella salina, and its cell density is 15-20 billion cells / ml.

[0031] The mealworms fed in the embodiment of the present invention are mealworm powder.

[0032] Example 1

[0033] An artificial seedling raising method of tiger dragon hybrid spot comprises the following steps:

[0034] (1) Choose an outdoor high-level pool with an area of ​​2000m 2 , the pond depth is 3.5m, the water level is 3.0m; 7-day-old tiger-dragon hybrid spotted fish fry are selected and released into the high-level pond, with a stocking density of about 7,000 fry / m 3 ;

[0035] (2) Rotifers were fed to the fish from 7 to 14 days old, and the density of rotifers was kept at 8 / mL. The rotifers were composed of SS-type rotifers and L-type rotifers, and the ratio of SS-type rotifers to L-type rotifers was 1:3. At the same time, the pool was inoculated with pyrenoid Chlorella solution and Dunaliella salina solution. The inoculation volume of pyrenoid Chlorella solution was 8 mL / m 2 The inoculation volume of Dunaliella salina liquid was 3 mL / m 2 ;

[0036] From 15 to 30 days old, feed Artemia and Mongoloid fleas, with Artemia maintained at a density of 12 / mL and M. mongoloid fleas maintained at a density of 4 / mL;

[0037] From 31 days to 50 days old, feed with M. mongolica and Tenebrio molitor. The density of M. mongolica was maintained at 18 / mL, and the daily feeding amount of Tenebrio molitor was about 2.5% of the total weight of the fry.

[0038] (3) When encountering heavy rain, feed nutrients within 24 hours after the rain, with the dosage of 1.5 mg / m 2 The nutrient agent includes the following raw materials in parts by weight: 15 g of Haematococcus pluvialis powder, 1.5 g of nicotinamide, 4 g of thiamine, 1.5 g of astaxanthin, 15 g of disodium EDTA, 150 g of ammonium sulfate, 35 g of superphosphate and 1 kg of water. The nutrient agent is diluted 1000 times with seawater before use.

[0039] Example 2

[0040] An artificial seedling raising method of tiger dragon hybrid spot comprises the following steps:

[0041] (1) Choose an outdoor high-level pool with an area of ​​2000m 2 , the pond depth is 3.5m, the water level is 3.0m; 7-day-old tiger-dragon hybrid spotted fish fry are selected and released into the high-level pond, with a stocking density of about 7,000 fry / m 3 ;

[0042] (2) Rotifers were fed to the fish from 7 to 14 days old. The density of rotifers was kept at 5 / mL. The rotifers were composed of SS-type rotifers and L-type rotifers. The ratio of SS-type rotifers to L-type rotifers was 1:4. At the same time, the pool was inoculated with Chlorella pyrenoidosa liquid and Dunaliella salina liquid. The inoculation volume of Chlorella pyrenoidosa liquid was 5 mL / mL. 2 The inoculation volume of Dunaliella salina liquid was 4 mL / m 2 ;

[0043] From 15 to 30 days old, feed Artemia and Mongoloid fleas, with Artemia maintained at a density of 11 / mL and M. mongoloid fleas maintained at a density of 5 / mL;

[0044] From 31 days to 50 days old, feed Moina mongolica and mealworms. The density of Moina mongolica was maintained at 15 / mL, and the daily feeding amount of mealworms was 3% of the total weight of the fry.

[0045] (3) When encountering heavy rain, feed nutrients within 24 hours after the rain, with the dosage of 2 mg / m 2The nutrient agent includes the following raw materials in parts by weight: 10g of Haematococcus pluvialis powder, 2g of nicotinamide, 5g of thiamine, 2g of astaxanthin, 20g of disodium EDTA, 200g of ammonium sulfate, 30g of superphosphate and 1kg of water. The nutrient agent is diluted 1000 times with seawater before use.

[0046] Example 3

[0047] An artificial seedling raising method of tiger dragon hybrid spot comprises the following steps:

[0048] (1) Choose an outdoor high-level pool with an area of ​​2000m 2 , the pond depth is 3.5m, the water level is 3.0m; 7-day-old tiger-dragon hybrid spotted fish fry are selected and released into the high-level pond, with a stocking density of about 7,000 fry / m 3 ;

[0049] (2) Rotifers were fed to the fish from 7 to 14 days old, and the density of rotifers was maintained at 9 / mL. The rotifers consisted of SS-type rotifers and L-type rotifers, and the ratio of SS-type rotifers to L-type rotifers was 1:2. At the same time, the pool was inoculated with Chlorella pyrenoidosa liquid and Dunaliella salina liquid. The inoculation volume of Chlorella pyrenoidosa liquid was 10 mL / m 2 The inoculation volume of Dunaliella salina liquid was 2 mL / m 2 ;

[0050] From 15 to 30 days old, feed Artemia and Mongoloid fleas, with Artemia maintained at a density of 13 / mL and M. mongoloid fleas maintained at a density of 3 / mL;

[0051] From 31 days to 50 days old, feed Moina mongolica and mealworms. The density of Moina mongolica was maintained at 20 / mL, and the daily feeding amount of mealworms was 2% of the total weight of the fry.

[0052] (3) When encountering heavy rain, feed nutrients within 24 hours after the rain, and the amount of nutrients added is 1 mg / m 2 The nutrient agent includes the following raw materials in parts by weight: 20g of Haematococcus pluvialis powder, 1g of nicotinamide, 3g of thiamine, 1g of astaxanthin, 10g of disodium EDTA, 100g of ammonium sulfate, 40g of superphosphate and 1kg of water. The nutrient agent is diluted 1000 times with seawater before use.

[0053] Comparative Example 1

[0054] The difference from the embodiment is that, from the 7th to the 14th day of age stage, the Dunaliella salina liquid inoculated in the pool is replaced with an equal amount of Chlorella pyrenoidosa liquid, that is, the entire pool is inoculated with Chlorella pyrenoidosa liquid. Specifically:

[0055] Rotifers were fed from 7 to 14 days old, and the density of rotifers was kept at 8 / mL. The rotifers were composed of SS-type rotifers and L-type rotifers, and the ratio of SS-type rotifers to L-type rotifers was 1:3. At the same time, the pool was inoculated with pyrenoid Chlorella liquid; the inoculation volume of pyrenoid Chlorella liquid was 11mL / m 2 ;

[0056] From 15 to 30 days old, feed Artemia and Mongoloid fleas, with Artemia maintained at a density of 12 / mL and M. mongoloid fleas maintained at a density of 4 / mL;

[0057] From 31 days to 50 days old, they were fed with M. mongolica and Tenebrio molitor. The density of M. mongolica was maintained at 18 / mL, and the daily feeding amount of Tenebrio molitor was 2.5% of the total weight of the fry.

[0058] When encountering heavy rain, feed nutrients within 24 hours after the rain, the dosage is 1.5mg / m 2 The nutrient agent includes the following raw materials in parts by weight: 15 g of Haematococcus pluvialis powder, 1.5 g of nicotinamide, 4 g of thiamine, 1.5 g of astaxanthin, 15 g of disodium EDTA, 150 g of ammonium sulfate, 35 g of superphosphate and 1 kg of water. The nutrient agent is diluted 1000 times with seawater before use.

[0059] Other management is consistent with Example 1.

[0060] Comparative Example 2

[0061] Different from the embodiment, in the 15-day to 30-day age stage, the feeding amount of Mongolica mongolica is replaced by an equal amount of Artemia spp., that is, Artemia spp. are fed entirely. Specifically:

[0062] Rotifers were fed from 7 to 14 days old, and the density of rotifers was kept at 8 / mL. The rotifers were composed of SS-type rotifers and L-type rotifers, and the ratio of SS-type rotifers to L-type rotifers was 1:3. At the same time, the pool was inoculated with pyrenoid Chlorella liquid and salina liquid; the inoculation volume of pyrenoid Chlorella liquid was 8mL / m 2 The inoculation volume of Dunaliella salina liquid was 3 mL / m 2 ;

[0063] From 15 to 30 days old, feed Artemia natans at a density of 16 / mL;

[0064] From 31 days to 50 days old, they were fed with M. mongolica and Tenebrio molitor. The density of M. mongolica was maintained at 18 / mL, and the daily feeding amount of Tenebrio molitor was 2.5% of the total weight of the fry.

[0065] When encountering heavy rain, feed nutrients within 24 hours after the rain, the dosage is 1.5mg / m 2The nutrient agent includes the following raw materials in parts by weight: 15 g of Haematococcus pluvialis powder, 1.5 g of nicotinamide, 4 g of thiamine, 1.5 g of astaxanthin, 15 g of disodium EDTA, 150 g of ammonium sulfate, 35 g of superphosphate and 1 kg of water. The nutrient agent is diluted 1000 times with seawater before use.

[0066] Other management is consistent with Example 1.

[0067] Comparative Example 3

[0068] Different from the embodiment, the feeding amount of mealworms from 31 days to 50 days old is replaced by an equal amount of fish meal. Specifically:

[0069] Rotifers were fed from 7 to 14 days old, and the density of rotifers was kept at 8 / mL. The rotifers were composed of SS-type rotifers and L-type rotifers, and the ratio of SS-type rotifers to L-type rotifers was 1:3. At the same time, the pool was inoculated with pyrenoid Chlorella liquid and salina liquid; the inoculation volume of pyrenoid Chlorella liquid was 8mL / m 2 The inoculation volume of Dunaliella salina liquid was 3 mL / m 2 ;

[0070] From 15 to 30 days old, feed Artemia and Mongoloid fleas, with Artemia maintained at a density of 12 / mL and M. mongoloid fleas maintained at a density of 4 / mL;

[0071] From 31 days to 50 days of age, the fry were fed with Moina mongolica and fish meal. The density of Moina mongolica was maintained at 18 / mL, and the daily feeding amount of fish meal was 2.5% of the total weight of the fry.

[0072] When encountering heavy rain, feed nutrients within 24 hours after the rain, the dosage is 1.5mg / m 2 The nutrient agent includes the following raw materials in parts by weight: 15 g of Haematococcus pluvialis powder, 1.5 g of nicotinamide, 4 g of thiamine, 1.5 g of astaxanthin, 15 g of disodium EDTA, 150 g of ammonium sulfate, 35 g of superphosphate and 1 kg of water. The nutrient agent is diluted 1000 times with seawater before use.

[0073] Other management is consistent with Example 1.

[0074] Comparative Example 4

[0075] Different from the embodiment, when encountering a rainstorm, the Haematococcus pluvialis powder in the nutrient formula is replaced with Spirulina powder within 24 hours after the rain.

[0076] Specifically: When encountering heavy rain, feed nutrients within 24 hours after the rain, and the dosage is 1.5mg / m 2The nutrient comprises the following raw materials in parts by weight: 15 g of spirulina powder, 1.5 g of nicotinamide, 4 g of thiamine, 1.5 g of astaxanthin, 15 g of disodium EDTA, 150 g of ammonium sulfate, 35 g of superphosphate and 1 kg of water. Other management is the same as that in Example 1.

[0077] Comparative Example 5

[0078] Different from the embodiment, when encountering heavy rain, the proportion of nutrients fed within 24 hours after the rain is different, specifically: when encountering heavy rain, the nutrients are fed within 24 hours after the rain, and the dosage is 1.5 mg / m 2 The nutrient agent is prepared by the following raw materials in parts by weight: 30g of Haematococcus pluvialis powder, 30g of nicotinamide, 30g of thiamine, 30g of astaxanthin, 30g of disodium EDTA, 30g of ammonium sulfate, 30g of superphosphate and 1kg of water. The nutrient agent is diluted 1000 times with seawater before use. Other management is the same as Example 1.

[0079] Application Examples

[0080] 7-day-old tiger-dragon hybrid spotted fish fry were selected and raised according to the methods of Examples 1-3 and Comparative Examples 1-5, respectively. After raising the fry to 50 days old, the survival rate of the fry and the size of the fry (average body length) were counted, and the number of mutations in the obtained fry was counted to calculate the fry mutation rate. The results are shown in Table 1-2.

[0081] Table 1 Seedling cultivation of tiger dragon hybrid spot

[0082]

[0083] It can be seen from Table 1 that among all the test groups, the seedling survival rate of Example 1-3 groups is higher, and the average body length is longer, the size is larger, and the seedling effect is better.

[0084] Table 2 Variation of seedling cultivation of tiger dragon hybrid spot

[0085]

[0086]

[0087] As can be seen from Table 2, compared with the comparative examples 1-5, the variation rate of the examples 1-3 is lower. The seedling raising method of the examples 1-3 can better ensure the seedling raising quality of the tiger dragon hybrid spot.

[0088] The above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An artificial seedling raising method for tiger dragon hybrid spot, characterized in that: The following steps are involved: (1) Select an outdoor high-level pond, select tiger-dragon hybrid spotted fish fry that are over 7 days old, and release the tiger-dragon hybrid spotted fish fry into the high-level pond; (2) Feed rotifers to the fish from 7 to 14 days old, and inoculate the pool with Chlorella pyrenoidosa and Dunaliella salina liquid; From 15 to 30 days old, feed Artemia and Mongoloid fleas; From 31 to 50 days old, they were fed with Moina mongolica and mealworms; (3) When encountering a rainstorm, feed a nutrient within 24 hours after the rain. The nutrient comprises the following raw materials in parts by weight: 10-20 g of Haematococcus pluvialis powder, 1-2 g of nicotinamide, 3-5 g of thiamine, 1-2 g of astaxanthin, 10-20 g of disodium EDTA, 100-200 g of ammonium sulfate, 30-40 g of superphosphate and 0.9-1.1 kg of water. The amount of the nutrient is 1-2 mg / m 2 .

2. The artificial seedling raising method of tiger dragon hybrid spot according to claim 1, is characterized in that, From 7 to 14 days old, the rotifer density is maintained at 5-10 / mL.

3. The artificial seedling raising method of the tiger dragon hybrid spot according to claim 1 or 2, characterized in that, The rotifers are composed of SS-type rotifers and L-type rotifers, and the number ratio of the SS-type rotifers to the L-type rotifers is 1:2-4.

4. The artificial seedling raising method of tiger dragon hybrid spot according to claim 1 is characterized in that, The inoculation volume of Chlorella pyrenoidosa liquid is 5-10mL / m 2 The inoculation volume of Dunaliella salina liquid is 2-4mL / m 2 ; The cell density of the Chlorella pyrenoidosa liquid is 25-30 billion cells / ml, and the cell density of the Dunaliella salina liquid is 15-20 billion cells / ml.

5. The artificial seedling raising method of tiger dragon hybrid spot according to claim 1 is characterized in that, From 15 to 30 days old, the density of Artemia is maintained at 10-15 / mL, and the density of Mongoloid flea is maintained at 3-5 / mL.

6. The artificial seedling raising method of the tiger dragon hybrid spot according to claim 1 or 5, characterized in that: From 31 days to 50 days of age, the density of Moina mongolica was maintained at 15-20 / mL, and the daily feeding amount of mealworms was 2%-3% of the total weight of the fry.

7. The artificial seedling raising method of tiger dragon hybrid spot according to claim 1 is characterized in that, Step (1): The stocking density of fry is 0.6-0.8 million / m 3 .

8. The artificial seedling raising method of the tiger dragon hybrid spot according to claim 1 or 7, characterized in that: The area of ​​the high-level pool is 1000-3000m 2 , the pool depth is 3-4m and the water level is 2.5-3.5m.

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