A breeding method for hybrid spotted spruce tiger

Abstract

This invention provides a method for raising hybrid grouper, relating to the field of grouper farming. The method for raising hybrid grouper of this invention includes the following steps: (1) Selecting hybrid grouper fry of 8-12cm size and placing them in a temporary holding pond, adding nutrients for temporary holding. The nutrient ingredients are: Dunaliella salina powder, astaxanthin, and coconut oil; (2) After the temporary holding period, transferring them to a rearing pond and feeding them with nutrient feed I daily for 2-4 months. The ingredients of nutrient feed I are: fish meal, coconut meal powder, soybean meal powder, corn flour, methionine iron, calcium dihydrogen phosphate, L-selenomethionine, Clostridium butyricum, and Bacillus subtilis; (3) After the second stage of rearing, feeding them with nutrient feed I and nutrient feed II daily. The ingredients of nutrient feed II are: allicin, Alpinia oxyphylla stems and leaves, purslane, Clostridium butyricum, Lactobacillus plantarum, and Lactobacillus rhamnosus, and harvesting adult fish. This method for raising hybrid grouper accelerates their growth rate, effectively improves the survival rate, and results in large-sized, highly nutritious adult fish.

Description

Technical Field

[0001] This invention relates to the field of grouper farming, and in particular to a method for farming hybrid grouper. Background Technology

[0002] Grouper, as an important marine fish resource in my country, has significant economic value and market demand, playing a pivotal role in the aquaculture industry with an annual demand for over 1 billion fry. To address these challenges, constructing a diversified germplasm bank for grouper is crucial. Collecting and preserving genetic resources from different strains provides a rich genetic foundation for subsequent breeding. Based on this, developing hybrid strains with superior traits and conducting in-depth analysis of their phenotypic and genetic characteristics can effectively identify germplasm resources with hybrid vigor. Furthermore, genetic analysis of the superior traits of hybrid offspring, identifying key genes and molecular markers, will provide strong technical support for grouper variety improvement. Systematic research on the large-scale breeding and industrial application of superior hybrid germplasm can not only improve the growth rate and stress resistance of grouper but also significantly improve fry survival rates and solve long-standing disease problems in the industry. Therefore, constructing a grouper variety improvement technology system and developing high-quality germplasm resources will provide an effective solution for enhancing the overall competitiveness and sustainable development of the industry. These studies are of great significance for promoting the healthy development of my country's grouper industry.

[0003] The freshwater grouper (Epinephelus Polyphekadion) is prized for its delicious flesh and strong disease resistance. The brown-spotted grouper (Epinephelus Fuscoguttatus) is known for its wide temperature tolerance, rapid growth, delicious flesh, and high nutritional value. The Epinephelus-Fuscoguttatus hybrid grouper is a hybrid breed produced by using the freshwater grouper as the female parent and the brown-spotted grouper as the male parent. However, the breeding cycle for the Epinephelus-Fuscoguttatus hybrid grouper is generally long, often requiring 15-16 months from stocking to sale. This not only increases breeding costs but also raises the risks. Furthermore, during the breeding process, ichthyophthirius multifiliis disease is prevalent in April, and dactylus infection is common in August. The survival rate of the Epinephelus-Fuscoguttatus hybrid grouper is generally around 70%. Summary of the Invention

[0004] In view of this, the present invention proposes a breeding method for hybrid spotted sable stag and tiger stag to solve the above problems.

[0005] The technical solution of this invention is implemented as follows: a method for breeding hybrid spotted sika deer, comprising the following steps:

[0006] (1) First stage: Select 8-12cm hybrid spotted fish fry and put them into a temporary holding pond. Add nutrients and temporarily hold them for 24-48 hours. The nutrients are made from the following raw materials in parts by weight: 40-60 parts of Dunaliella salina powder, 25-35 parts of astaxanthin, and 15-25 parts of coconut oil.

[0007] (2) Second stage: After the temporary holding period ends, the animals are transferred to the breeding pond and fed with nutrient feed I every day for 2-4 months. The nutrient feed I is made from the following raw materials in parts by weight: 25-35 parts fish meal, 12-18 parts coconut meal powder, 15-25 parts soybean meal powder, 8-12 parts corn flour, 4-6 parts methionine iron, 4-6 parts calcium dihydrogen phosphate, 1-3 parts L-selenomethionine, 5-10 parts Clostridium butyricum, and 4-6 parts Bacillus subtilis.

[0008] (3) Third stage: After the second stage of breeding is completed, feed the fish with nutrient feed I and nutrient feed II every day. Nutrient feed II is made from the following raw materials in parts by weight: 8-12 parts of allicin, 12-18 parts of Alpinia oxyphylla stems and leaves, 25-35 parts of purslane, 8-12 parts of Clostridium butyricum, 15-25 parts of Lactobacillus plantarum, and 12-18 parts of Lactobacillus rhamnosus. Harvest the fish when they reach 500g or more.

[0009] Furthermore, the area of ​​the temporary holding tank is 100-200m². 2 The release density is 10-60 fish / m³. 2 The water depth is 1-1.2m, the water temperature is between 20-23℃, and the pH value of the water is between 6.8-7.2.

[0010] Furthermore, the area of ​​the aquaculture pond is 500-1000m². 2 The release density is 5-40 fish / m³ 2 The water depth is 1.2-1.5m, the water temperature is between 20-28℃, and the pH value of the water is between 6.5-7.5.

[0011] Furthermore, in the first stage, the daily amount of the nutrient solution is 1% to 2% of the body weight of the jujube tiger hybrid.

[0012] Furthermore, in the second stage, the daily feeding amount of the nutrient feed I is 2% to 4% of the body weight of the jujube tiger hybrid.

[0013] Furthermore, in the third stage, the total daily feeding amount of nutrient feed I and nutrient feed II is 4% to 6% of the body weight of the jujube-tiger hybrid, and the feeding weight ratio of nutrient feed I and nutrient feed II is 1:0.2-0.4.

[0014] Furthermore, the nutrient is prepared from the following raw materials in parts by weight: 50 parts Dunaliella salina powder, 30 parts astaxanthin, and 20 parts coconut oil.

[0015] Furthermore, the nutrient I is made from the following raw materials in parts by weight: 30 parts fish meal, 15 parts coconut meal, 20 parts soybean meal, 10 parts corn flour, 5 parts methionine iron, 5 parts dihydrogen phosphate, 2 parts L-selenomethionine, 8 parts Clostridium butyricum, and 5 parts Saccharomyces cerevisiae.

[0016] Furthermore, the nutrient II is prepared from the following raw materials in parts by weight: 10 parts allicin, 15 parts Alpinia oxyphylla stems and leaves, 30 parts purslane, 10 parts Clostridium butyricum, 20 parts Lactobacillus plantarum, and 15 parts Lactobacillus rhamnosus.

[0017] Furthermore, the preparation method of the nutrient feed I includes the following steps: take fish meal, coconut meal powder, soybean meal powder, and corn flour, add Clostridium butyricum and Bacillus subtilis and ferment at 30-38℃ for 48-96 hours, add methionine iron, calcium dihydrogen phosphate, and L-selenomethionine, mix evenly, and obtain nutrient feed I.

[0018] Furthermore, the preparation method of the nutrient material II includes the following steps: taking the stems and leaves of Alpinia oxyphylla and Portulaca oleracea, adding Clostridium butyricum, Lactobacillus plantarum and Lactobacillus rhamnosus, fermenting at 35-40℃ for 24-48h, adding allicin after fermentation, mixing evenly, and obtaining nutrient material II.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] (1) The method of this invention for raising and harvesting hybrid grouper effectively reduces the incidence of Ichthyophthirius multifiliis and Dactylogyrus disease during the breeding cycle, effectively improves the survival rate of hybrid grouper, and the harvested adult hybrid grouper is larger in size, with high protein content and high nutritional value.

[0021] (2) In this invention, Dunaliella salina powder, astaxanthin, and coconut oil are used as nutrients for temporary rearing. After the temporary rearing is completed, the animals are transferred to a breeding pond and fed with nutrient feed I. After 2-4 months of breeding, nutrient feed I and nutrient feed II are fed at the same time. Nutrient feed I is made by fermenting fish meal, coconut meal powder, soybean meal powder, corn flour, methionine iron, calcium dihydrogen phosphate, L-selenomethionine with Clostridium butyricum and Bacillus subtilis. Nutrient feed II is made by fermenting allicin, Alpinia oxyphylla stems and leaves, purslane, Clostridium butyricum, Lactobacillus plantarum, and Lactobacillus rhamnosus. This invention effectively accelerates the growth rate of the hybrid spotted juvenile ... Detailed Implementation

[0022] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0023] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0024] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0025] The embodiments or comparative examples of this invention use the stems and leaves of the plant Alpinia oxyphylla Miq.;

[0026] In the embodiments or comparative examples of this invention, the whole plant of Portulaca oleracea Linn. is used.

[0027] In the embodiments or comparative examples of this invention, the Bacillus subtilis, Clostridium butyricum, Lactobacillus plantarum, and Lactobacillus rhamnosus used were all bacterial suspensions (prepared) with a viable bacterial concentration of approximately 1 billion CFU / mL, and the bacterial strains were all purchased from the Guangdong Provincial Microbial Culture Collection Center.

[0028] Among them, Bacillus subtilis, preservation number GDMCC NO.1.131;

[0029] Clostridium butyricum, accession number GDMCC NO.1.2756;

[0030] Lactiplantibacillus plantarum, accession number GDMCC NO.1.140;

[0031] Lactobacillus rhamnosus, accession number GDMCC NO.1.1732.

[0032] Example 1

[0033] A method for breeding hybrid spotted sable tigers includes the following steps:

[0034] (1) First stage:

[0035] Select healthy 8-12cm hybrid spotted catfish fry and release them into a 200m² holding tank. 2 The release density was 50 fish / m³. 2 The water depth is 1-1.2m, the water temperature is between 20-23℃, and the pH value of the water is between 6.8-7.2. The animals are temporarily kept in a nutrient solution for 24 hours. The daily feeding amount of the nutrient solution is about 1.5% of the body weight of the hybrid spotted tiger. The nutrient solution is made from the following raw materials in parts by weight: 50 parts of Dunaliella salina powder, 30 parts of astaxanthin, and 20 parts of coconut oil. The Dunaliella salina powder, astaxanthin, and coconut oil are mixed evenly to prepare the nutrient solution.

[0036] (2) Second stage:

[0037] After the temporary holding period, they were transferred to a rearing pond with an area of ​​500m². 2 The release density was 20 fish / m³. 2 The water depth is 1.2-1.5m, the water temperature is between 20-28℃, and the pH value is between 6.5-7.5. The fish are fed Nutrient I daily for 3 months. The daily feeding amount of Nutrient I is approximately 3.0% of the body weight of the hybrid tiger scorpion. Nutrient I is made from the following ingredients in parts by weight: 30 parts fish meal, 15 parts coconut meal, 20 parts soybean meal, 10 parts corn flour, 5 parts methionine iron, 5 parts calcium dihydrogen phosphate, 2 parts L-selenomethionine, 8 parts Clostridium butyricum, and 5 parts Bacillus subtilis. The preparation method of Nutrient I includes the following steps: Take fish meal, coconut meal, soybean meal, and corn flour, add Clostridium butyricum and Bacillus subtilis, and ferment at 35℃±2℃ for 48 hours. After fermentation, add methionine iron, calcium dihydrogen phosphate, and L-selenomethionine, mix evenly, and obtain Nutrient I.

[0038] (3) Third stage:

[0039] After 3 months of rearing, the fish were fed nutrient feed I and nutrient feed II daily. The total daily feeding amount of nutrient feed I and nutrient feed II was approximately 5.0% of the body weight of the hybrid grouper. The weight ratio of nutrient feed I to nutrient feed II was 1:0.3. Nutrient feed II was prepared from the following raw materials in parts by weight: 10 parts allicin, 15 parts Alpinia oxyphylla stems and leaves, 30 parts purslane, 10 parts Clostridium butyricum, 20 parts Lactobacillus plantarum, and 15 parts Lactobacillus rhamnosus. The preparation method of nutrient feed II included the following steps: taking Alpinia oxyphylla stems and leaves and purslane, adding Clostridium butyricum, Lactobacillus plantarum, and Lactobacillus rhamnosus, and fermenting at 37℃±2℃ for 24 hours. After fermentation, allicin was added and mixed evenly to obtain nutrient feed II. The fish were harvested when they reached a weight of over 500g.

[0040] Example 2

[0041] A method for breeding hybrid spotted sable tigers includes the following steps:

[0042] (1) First stage:

[0043] Select healthy 8-12cm hybrid spotted catfish fry and release them into a 200m² holding tank. 2 The release density was 50 fish / m³. 2 The water depth is 1-1.2m, the water temperature is between 20-23℃, and the pH value of the water is between 6.8-7.2. The animals are temporarily kept in a nutrient solution for 24-48 hours. The daily feeding amount of the nutrient solution is about 1.5% of the body weight of the hybrid spotted tiger. The nutrient solution is made from the following raw materials in parts by weight: 45 parts of Dunaliella salina powder, 35 parts of astaxanthin, and 25 parts of coconut oil. The Dunaliella salina powder, astaxanthin, and coconut oil are mixed evenly to prepare the nutrient solution.

[0044] (2) Second stage:

[0045] After the temporary holding period, they were transferred to a rearing pond with an area of ​​500m². 2 The release density was 20 fish / m³. 2 The water depth is 1.2-1.5m, the water temperature is between 20-28℃, and the pH value is between 6.5-7.5. The fish are fed Nutrient I daily for 3 months. The daily feeding amount of Nutrient I is approximately 3.0% of the body weight of the hybrid tiger scorpion. Nutrient I is made from the following ingredients in parts by weight: 27 parts fish meal, 18 parts coconut meal powder, 25 parts soybean meal powder, 8 parts corn flour, 4 parts methionine iron, 6 parts calcium dihydrogen phosphate, 3 parts L-selenomethionine, 5 parts Clostridium butyricum, and 4 parts Bacillus subtilis. The preparation method of Nutrient I includes the following steps: Take fish meal, coconut meal powder, soybean meal powder, and corn flour, add Clostridium butyricum and Bacillus subtilis, and ferment at 35℃±2℃ for 48 hours. After fermentation, add methionine iron, calcium dihydrogen phosphate, and L-selenomethionine, mix evenly, and obtain Nutrient I.

[0046] (3) Third stage:

[0047] After 3 months of rearing, the fish were fed nutrient feed I and nutrient feed II daily. The total daily feeding amount of nutrient feed I and nutrient feed II was approximately 5.0% of the body weight of the hybrid grouper. The weight ratio of nutrient feed I to nutrient feed II was 1:0.2. Nutrient feed II was prepared from the following raw materials in parts by weight: 8 parts allicin, 18 parts Alpinia oxyphylla stems and leaves, 25 parts purslane, 12 parts Clostridium butyricum, 25 parts Lactobacillus plantarum, and 12 parts Lactobacillus rhamnosus. The preparation method of nutrient feed II included the following steps: taking Alpinia oxyphylla stems and leaves and purslane, adding Clostridium butyricum, Lactobacillus plantarum, and Lactobacillus rhamnosus, and fermenting at 37℃±2℃ for 24 hours. After fermentation, allicin was added and mixed evenly to obtain nutrient feed II. The fish were harvested when they reached a weight of over 500g.

[0048] Example 3

[0049] A method for breeding hybrid spotted sable tigers includes the following steps:

[0050] (1) First stage:

[0051] Select healthy 8-12cm hybrid spotted catfish fry and release them into a 200m² holding tank. 2 The release density was 50 fish / m³. 2 The water depth is 1-1.2m, the water temperature is between 20-23℃, and the pH value of the water is between 6.8-7.2. The animals are temporarily kept in a nutrient solution for 24-48 hours. The daily feeding amount of the nutrient solution is about 1.5% of the body weight of the hybrid spotted tiger. The nutrient solution is made from the following raw materials in parts by weight: 50 parts of Dunaliella salina powder, 35 parts of astaxanthin, and 15 parts of coconut oil. The Dunaliella salina powder, astaxanthin, and coconut oil are mixed evenly to prepare the nutrient solution.

[0052] (2) Second stage:

[0053] After the temporary holding period, they were transferred to a rearing pond with an area of ​​500m². 2 The release density was 20 fish / m³. 2 The water depth is 1.2-1.5m, the water temperature is between 20-28℃, and the pH value is between 6.5-7.5. The fish are fed Nutrient I daily for 3 months. The daily amount of Nutrient I is approximately 3% of the body weight of the hybrid tiger scorpion. Nutrient I is made from the following ingredients in parts by weight: 34 parts fish meal, 12 parts coconut meal powder, 15 parts soybean meal powder, 12 parts corn flour, 6 parts methionine iron, 4 parts calcium dihydrogen phosphate, 1 part L-selenomethionine, 10 parts Clostridium butyricum, and 6 parts Bacillus subtilis. The preparation method of Nutrient I includes the following steps: Take fish meal, coconut meal powder, soybean meal powder, and corn flour, add Clostridium butyricum and Bacillus subtilis, and ferment at 35℃±2℃ for 48 hours. After fermentation, add methionine iron, calcium dihydrogen phosphate, and L-selenomethionine, mix evenly, and obtain Nutrient I.

[0054] (3) Third stage:

[0055] After 3 months of rearing, the fish were fed nutrient feed I and nutrient feed II daily. The total daily feeding amount of nutrient feed I and nutrient feed II was approximately 5.0% of the body weight of the hybrid grouper. The weight ratio of nutrient feed I to nutrient feed II was 1:0.4. Nutrient feed II was prepared from the following raw materials in parts by weight: 12 parts allicin, 12 parts Alpinia oxyphylla stems and leaves, 35 parts purslane, 8 parts Clostridium butyricum, 25 parts Lactobacillus plantarum, and 12 parts Lactobacillus rhamnosus. The preparation method of nutrient feed II included the following steps: taking Alpinia oxyphylla stems and leaves and purslane, adding Clostridium butyricum, Lactobacillus plantarum, and Lactobacillus rhamnosus, and fermenting at 37℃±2℃ for 24 hours. After fermentation, allicin was added and mixed evenly to obtain nutrient feed II. The fish were harvested when they reached a weight of over 500g.

[0056] Comparative Example 1

[0057] The main difference from Example 1 is that in the first stage, the nutrient agent, Dunaliella salina powder, is replaced with Spirulina powder, and coconut oil is replaced with soybean oil. Specifically, the nutrient agent in the first stage is prepared from the following raw materials in parts by weight: 50 parts Spirulina powder, 35 parts Astaxanthin, and 15 parts Soybean oil. Other processing is the same as in Example 1.

[0058] Comparative Example 2

[0059] The main difference from Example 1 is that Clostridium butyricum was not added in the second stage, and Bacillus subtilis was added instead.

[0060] Specifically, this nutrient feed I is made from the following raw materials in parts by weight: 30 parts fish meal, 15 parts coconut meal, 20 parts soybean meal, 10 parts corn flour, 5 parts methionine iron, 5 parts calcium dihydrogen phosphate, 2 parts L-selenomethionine, and 13 parts Bacillus subtilis. Other treatments are the same as in Example 1.

[0061] Comparative Example 3

[0062] The main difference from Example 1 is that Lactobacillus rhamnosus was not added in the third stage, but the amount of Lactobacillus plantarum was increased by the same amount. Specifically, in the third stage, the nutrient II was prepared from the following raw materials in parts by weight: 10 parts allicin, 15 parts Alpinia oxyphylla stems and leaves, 30 parts purslane, 10 parts Clostridium butyricum, and 35 parts Lactobacillus plantarum. Other treatments were the same as in Example 1.

[0063] Comparative Example 4

[0064] The main difference from Example 1 is that the third stage did not include the stems and leaves of Alpinia oxyphylla, but the amount of purslane was increased by the same amount. Specifically, in the third stage, the nutrient II was prepared from the following raw materials in parts by weight: 10 parts allicin, 45 parts purslane, 10 parts Clostridium butyricum, 20 parts Lactobacillus plantarum, and 15 parts Lactobacillus rhamnosus. Other treatments were the same as in Example 1.

[0065] Comparative Example 5

[0066] The main difference from Example 1 is that purslane was not added in the third stage, but the amount of Alpinia oxyphylla stems and leaves was increased by the same amount. Specifically, in the third stage, the nutrient II was prepared from the following raw materials in parts by weight: 10 parts allicin, 45 parts Alpinia oxyphylla stems and leaves, 10 parts Clostridium butyricum, 20 parts Lactobacillus plantarum, and 15 parts Lactobacillus rhamnosus. Other treatments were the same as in Example 1.

[0067] Test case

[0068] The experiments were conducted according to the methods of Examples 1-3 and Comparative Examples 1-5, with experimental groups 1-8, each containing 10,000 fish. 8-12cm hybrid spotted grouper fry were selected, and the experiment began in January 2023. Adult fish were harvested after one year of rearing. The incidence rates of Ichthyophthirius multifiliis and Dactylogyrus diseases were recorded during the rearing period; the survival rate of healthy adult fish was recorded; the average size of adult hybrid spotted grouper was calculated; and the protein content of the adult fish was tested.

[0069] Survival rate of healthy adult fish (%) = (Number of healthy adult surviving juvenile tiger bream ÷ Number of juvenile tiger bream fry released) × 100%;

[0070] Average size: 100 randomly selected juvenile tiger hybrid spotted juveniles were weighed and the average weight (g / juvenile) was calculated.

[0071] Protein content: determined by the Kjeldahl method in GB 5009.5-2016 National Food Safety Standard for Protein in Food.

[0072] The results are shown in Table 1 below:

[0073]

[0074] Table 1 above shows that the hybrid grouper of *Ichthyophthirius multifiliis* harvested using the methods in Examples 1-3 effectively reduced the incidence of Ichthyophthirius multifiliis and *Gyrodactylus multifiliis* during the rearing period, effectively improved the survival rate, and resulted in larger harvested adult fish with high protein content and nutritional value. Comparative Examples 1-5, however, showed less satisfactory rearing results.

[0075] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for breeding hybrid spotted spruce tiger, characterized in that, Includes the following steps: (1) First stage: Select 8-12cm hybrid spotted grouper fry and put them into a temporary holding pond. Add nutrients and temporarily hold them for 24-48 hours. The nutrients are made from the following raw materials in parts by weight: 40-60 parts of Dunaliella salina powder, 25-35 parts of astaxanthin, and 15-25 parts of coconut oil. The daily feeding amount of the nutrients is 1% to 2% of the body weight of the hybrid spotted grouper. (2) Second stage: After the temporary holding period ends, the fish are transferred to the breeding pond and fed with nutrient feed I for 2-4 months. The nutrient feed I is made from the following raw materials in parts by weight: 25-35 parts fish meal, 12-18 parts coconut meal powder, 15-25 parts soybean meal powder, 8-12 parts corn flour, 4-6 parts methionine iron, 4-6 parts calcium dihydrogen phosphate, 1-3 parts L-selenomethionine, 5-10 parts butyric acid clostridium, and 4-6 parts Bacillus subtilis. The daily feeding amount of nutrient feed I is 2% to 4% of the body weight of the juvenile tiger hybrid. The preparation method of nutrient feed I includes the following steps: take fish meal, coconut meal powder, soybean meal powder, and corn flour, add butyric acid clostridium and Bacillus subtilis and ferment at 30-38℃ for 48-96 hours. After fermentation, add methionine iron, calcium dihydrogen phosphate, and L-selenomethionine, mix evenly, and obtain nutrient feed I. (3) Third stage: After the second stage of breeding is completed, feed nutrient I and nutrient II every day. The total daily feeding amount of nutrient I and nutrient II is 4% to 6% of the body weight of the hybrid juvenile ...

2. The method for breeding hybrid spotted spruce tigers according to claim 1, characterized in that, The area of ​​the temporary holding tank is 100-200m². 2 The release density is 10-60 fish / m³. 2 The water depth is 1-1.2m, the water temperature is between 20-23℃, and the pH value of the water is between 6.8-7.

2.

3. The method for breeding hybrid spotted spruce tigers according to claim 1, characterized in that, The area of ​​the aquaculture pond is 500-1000m². 2 The release density is 5-40 fish / m³ 2 The water depth is 1.2-1.5m, the water temperature is between 20-28℃, and the pH value of the water is between 6.5-7.

5.

4. The method for raising hybrid spotted spruce tigers according to claim 1, characterized in that, The nutrient solution is made from the following raw materials in parts by weight: 50 parts Dunaliella salina powder, 30 parts astaxanthin, and 20 parts coconut oil.

5. The method for breeding hybrid spotted spruce tigers according to claim 1, characterized in that, Nutritional feed I is made from the following ingredients in parts by weight: 30 parts fish meal, 15 parts coconut meal, 20 parts soybean meal, 10 parts corn flour, 5 parts methionine iron, 5 parts dihydrogen phosphate, 2 parts L-selenomethionine, 8 parts Clostridium butyricum, and 5 parts Saccharomyces cerevisiae; Nutritional feed II is made from the following ingredients in parts by weight: 10 parts allicin, 15 parts Alpinia oxyphylla stems and leaves, 30 parts purslane, 10 parts Clostridium butyricum, 20 parts Lactobacillus plantarum, and 15 parts Lactobacillus rhamnosus.

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