Method and device for controlling cell fluid dispensing time

Abstract

The application relates to the field of cell liquid sub-packaging, and provides a cell liquid sub-packaging time control method and device. The method comprises the following steps: controlling cell liquid to be injected into a mixing temperature control device at a specific flow rate; determining a sub-packaging flow rate of the cell liquid according to the capacity of a sub-packaging bag and a preset sub-packaging time; and controlling the cell liquid in the mixing temperature control device to flow in a sub-packaging pipeline at the sub-packaging flow rate according to the specific flow rate and the capacity of the cell liquid in the mixing temperature control device. The cell liquid sub-packaging time control method and device provided in the application can ensure that the sub-packaging process is continuous and uninterrupted, ensure that the sub-packaging time can be strictly controlled within the preset time, and then the sub-packaging efficiency of the cell liquid can be improved by shortening the preset time, and short-time sub-packaging of large-capacity cell liquid can be realized.

Description

Technical Field

[0001] The present application relates to the technical field of cell fluid packaging, and in particular to a method and device for controlling cell fluid packaging time. Background Art

[0002] As the field of universal cell therapy continues to expand, the demand for cell therapy products has also increased significantly. These products are usually packaged in the form of cell fluids in different sizes of packaging bags to meet different usage needs.

[0003] Typically, the cell fluid packaging process includes a mixing stage and a packaging stage. However, in traditional cell fluid packaging methods, the mixing stage and the packaging stage are two independent process stages. This means that the total amount of cell fluid to be packaged must be mixed before packaging can begin. For large-volume cell fluid packaging, the mixing time and the packaging time are long and difficult to strictly control, resulting in very low packaging efficiency. Summary of the Invention

[0004] The embodiments of the present application provide a method and device for controlling the time of cell fluid packaging, which is used to solve the technical problem that most of the traditional cell fluid packaging is manual operation and the efficiency of large-volume cell fluid packaging is very low.

[0005] In a first aspect, an embodiment of the present application provides a method for controlling the time of cell fluid packaging, comprising:

[0006] Control the cell fluid to be injected into the mixing temperature control device at a specific flow rate;

[0007] Determine the cell solution dispensing flow rate based on the capacity of the dispensing bag and the preset dispensing time;

[0008] According to the specific flow rate and the volume of the cell fluid in the mixing and temperature control device, controlling the cell fluid in the mixing and temperature control device to flow in the filling pipeline at the filling flow rate;

[0009] The sub-packaging pipeline is a pipeline between the end of the output pipeline of the mixing temperature control device and the sub-packaging bag, and the sub-packaging bag is connected to the sub-packaging pipeline.

[0010] In one embodiment, controlling the cell fluid in the mixing temperature control device to flow in the filling pipeline at the filling flow rate according to the specific flow rate and the volume of the cell fluid in the mixing temperature control device includes:

[0011] According to the linear relationship between the sum of the subpackaging flow rates and the specific flow rate, the cell fluid in the mixing temperature control device is controlled to flow in the subpackaging pipeline at the subpackaging flow rate; wherein the sum of the subpackaging flow rates is the sum of the subpackaging flow rates of different subpackaging pipelines, and the linear relationship coefficient between the sum of the subpackaging flow rates and the specific flow rate is linearly related to the volume of the cell fluid in the mixing temperature control device.

[0012] In one embodiment, the specific flow rate is a flow rate at which the Reynolds number of the cell solution in the mixing and temperature control device reaches a critical value of turbulence.

[0013] In one embodiment, before controlling the cell fluid in the mixing temperature control device to flow in the filling pipeline at the filling flow rate, the method includes:

[0014] When the volume of the cell fluid in the mixing temperature control device reaches a volume threshold, the subpackaging pipeline is controlled to open.

[0015] In one embodiment, before controlling the cell fluid in the mixing temperature control device to flow in the filling pipeline at the filling flow rate, the method includes:

[0016] The sterile filter is controlled to open and connect the sub-packaging pipeline to the atmosphere; the sterile filter is arranged at the end of the sub-packaging pipeline.

[0017] In one embodiment, before controlling the cell fluid in the mixing temperature control device to flow in the filling pipeline at the filling flow rate, the method includes:

[0018] The internal pressure of the sub-packaging pipeline is monitored in real time by a pressure sensor, and the internal pressure of the sub-packaging pipeline is maintained in a negative pressure state by controlling the opening and closing of the sterile filter; the sterile filter is arranged at the end of the sub-packaging pipeline, and the pressure sensor is connected to the end of the sub-packaging pipeline via the sterile filter.

[0019] In one embodiment, the inner diameter of the output pipeline of the mixing temperature control device is the sum of the inner diameters of the multiple filling pipelines.

[0020] In a second aspect, an embodiment of the present application provides a cell fluid dispensing time control device, comprising:

[0021] A specific flow rate determination module is used to control the cell fluid to be injected into the mixing temperature control device at a specific flow rate;

[0022] The packaging flow rate determination module is used to determine the packaging flow rate of the cell solution according to the capacity of the packaging bag and the preset packaging time;

[0023] A subpackaging control module is configured to control the cell fluid in the mixing and temperature control device to flow in the subpackaging pipeline at the subpackaging flow rate according to the specific flow rate and the volume of the cell fluid in the mixing and temperature control device;

[0024] The sub-packaging pipeline is a pipeline between the end of the output pipeline of the mixing temperature control device and the sub-packaging bag, and the sub-packaging bag is connected to the sub-packaging pipeline.

[0025] In a third aspect, an embodiment of the present application provides an electronic device comprising a processor and a memory storing a computer program, wherein when the processor executes the program, the steps of the cell fluid packaging time control method described in the first aspect are implemented.

[0026] In a fourth aspect, an embodiment of the present application provides a non-transitory computer-readable storage medium, comprising a computer program, which, when executed by a processor, implements the steps of the cell fluid packaging time control method described in the first aspect.

[0027] The cell fluid packaging time control method and device provided in the present application first controls the cell fluid to be injected into the mixing temperature control device at a specific flow rate, then determines the packaging flow rate of the cell fluid based on the capacity of the packaging bag and the preset packaging time, and finally controls the cell fluid in the mixing temperature control device to flow in the packaging pipeline at the packaging flow rate based on the specific flow rate and the capacity of the cell fluid in the mixing temperature control device. Since the packaging flow rate is determined according to the capacity of the packaging bag and the preset packaging time, the packaging bag is connected to the packaging pipeline. Therefore, the packaging flow rate is the flow rate that can meet the requirements for completing the packaging of the packaging bag within the preset packaging time. The packaging flow rate is controlled according to the specific flow rate and the volume of the cell fluid in the mixing temperature control device. The packaging flow rate can be maintained by adjusting the cell fluid source factors, that is, the specific flow rate and the volume of the cell fluid that has been mixed and can be used for packaging, thereby ensuring that the mixing stage and the packaging stage are carried out simultaneously and continuously during the packaging process, thereby ensuring that the packaging time can be strictly controlled within the preset time, and then the packaging efficiency of the cell fluid can be improved by shortening the preset time, and short-time packaging of large-volume cell fluid can be achieved. Practice has shown that under the same other packaging conditions, the packaging time of this application can be shortened by about 80% compared with the traditional cell fluid packaging method. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions in the present application or the prior art, a brief introduction will be given below to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0029] Figure 1This is one of the flow diagrams of the cell solution packaging time control method provided in the embodiments of the present application;

[0030] Figure 2 The embodiment of this application provides Figure 1 Schematic diagram of the corresponding cell solution packaging time control system;

[0031] Figure 3 A schematic diagram of the structure of the cell fluid dispensing time control device provided in an embodiment of the present application;

[0032] Figure 4 It is a structural diagram of an electronic device provided in an embodiment of the present application.

[0033] Reference numerals:

[0034] 1-Mixing temperature control device; 2-Packaging bag; 3-Packaging pipeline; 4-Output pipeline; 5-Sterile filter; 6-Pressure sensor; 7-Sample bag; 8-First peristaltic pump; 9-Second peristaltic pump; 10-Third peristaltic pump; 11-Fourth peristaltic pump. DETAILED DESCRIPTION

[0035] To make the objectives, technical solutions, and advantages of this application more clear, the technical solutions in this application will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of this application. Obviously, the described embodiments are only part of the embodiments of this application, not all of them. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of this application.

[0036] Figure 1 This is one of the flow diagrams of the cell solution packaging time control method provided in the embodiments of the present application;

[0037] Figure 2 The embodiment of this application provides Figure 1 Schematic diagram of the corresponding cell solution packaging time control system;

[0038] Reference Figure 1-2 , the present application embodiment provides a method for controlling the time of cell fluid packaging, which may include:

[0039] 101. Control the cell fluid to be injected into the mixing temperature control device at a specific flow rate;

[0040] 102. Determine the filling flow rate of the cell solution according to the capacity of the filling bag and the preset filling time;

[0041] 103. According to the specific flow rate and the volume of the cell fluid in the mixing temperature control device, the cell fluid in the mixing temperature control device is controlled to flow in the filling pipeline at the filling flow rate.

[0042] The sub-packaging pipeline 3 is a pipeline between the end of the output pipeline 4 of the mixing temperature control device 1 and the sub-packaging bag 2 , and the sub-packaging bag 2 is connected to the sub-packaging pipeline 3 .

[0043] In step 101 , the flow rate of the cell fluid injected from the sample bag 7 into the mixing temperature control device 1 , ie, the specific flow rate, can be controlled by controlling the rotation speed of the first peristaltic pump 8 between the sample bag 7 and the mixing temperature control device 1 .

[0044] It should be noted that the flow rate in this embodiment refers to the flow rate of the cell fluid per unit time.

[0045] In step 102, the number of dispensing bags 2 can be one or more, and this is not limited herein. If there is only one dispensing bag 2, the dispensing flow rate of the cell slurry can be determined based on the ratio of the capacity of the dispensing bag 2 to the preset dispensing time. If there are multiple dispensing bags 2, and each dispensing bag 2 has a different preset dispensing time, the dispensing flow rate of the cell slurry injected into each dispensing bag 2 can be determined based on the capacity and preset dispensing time of each dispensing bag 2.

[0046] In step 103, the number of subpackaging pipelines 3 can be one or more, which is not limited here. In this embodiment, the number of subpackaging pipelines 3 is three. The flow rates of the second peristaltic pump 9, the third peristaltic pump 10 and the fourth peristaltic pump 11 on these three subpackaging pipelines can be controlled respectively to control the cell fluid in the mixing temperature control device 1 to flow in the corresponding subpackaging pipeline at a subpackaging flow rate corresponding to the subpackaging bag to be subpackaged, and thus injected into the subpackaging bag to be subpackaged at the subpackaging flow rate. Among them, subpackaging bags with the same subpackaging flow rate can be set in the same subpackaging pipeline for simultaneous subpackaging to improve the subpackaging efficiency.

[0047] It should be noted that the mixing temperature control device 1 in this embodiment can mix the cell fluid and adjust or control the environment of the cell fluid by heating or cooling the cell fluid to meet the packaging requirements of the cell fluid and ensure the activity and viability of the cells.

[0048] The cell fluid packaging time control method provided in this embodiment first controls the cell fluid to be injected into the mixing temperature control device at a specific flow rate, then determines the cell fluid packaging flow rate based on the capacity of the packaging bag and the preset packaging time, and finally controls the cell fluid in the mixing temperature control device to flow in the packaging pipeline at the packaging flow rate based on the specific flow rate and the capacity of the cell fluid in the mixing temperature control device. Since the packaging flow rate is determined according to the capacity of the packaging bag and the preset packaging time, the packaging bag is connected to the packaging pipeline. Therefore, the packaging flow rate is the flow rate that can meet the requirements for completing the packaging of the packaging bag within the preset packaging time. The packaging flow rate is controlled according to the specific flow rate and the volume of the cell fluid in the mixing temperature control device. The packaging flow rate can be maintained by adjusting the cell fluid source factors, that is, the specific flow rate and the volume of the cell fluid that has been mixed and can be used for packaging, thereby ensuring that the mixing stage and the packaging stage are carried out simultaneously and continuously during the packaging process, thereby ensuring that the packaging time can be strictly controlled within the preset time, and then the packaging efficiency of the cell fluid can be improved by shortening the preset time, and short-time packaging of large-volume cell fluid can be achieved. Practice has shown that under the same other packaging conditions, the packaging time of this application can be shortened by about 80% compared with the traditional cell fluid packaging method.

[0049] Reference Figure 2 In one embodiment, according to a specific flow rate and the volume of the cell fluid in the mixing and temperature control device 1, controlling the cell fluid in the mixing and temperature control device 1 to flow in the filling pipeline 3 at a filling flow rate may include:

[0050] According to the linear relationship between the sum of the subpackaging flow rates and the specific flow rate, the cell fluid in the mixing temperature control device 1 is controlled to flow in the subpackaging pipeline 3 at the subpackaging flow rate;

[0051] The sum of the subpackaging flow rates is the sum of the subpackaging flow rates of different subpackaging pipelines 3 , and the linear relationship coefficient between the sum of the subpackaging flow rates and the specific flow rate is linearly related to the volume of the cell fluid in the mixing temperature control device 1 .

[0052] The linear relationship between the sum of the filling flow rates and the specific flow rate can be expressed as follows:

[0053] v1=k(v2+v3+v4+…+v n+1 ); (2-1)

[0054] Among them, v1 is the specific flow rate, v2 to v n+1 are n filling flow rates corresponding to n filling pipelines, and k is the linear relationship coefficient between the sum of the filling flow rates and the specific flow rate.

[0055] Formula (2-1) also has:

[0056] k=αV-d; (2-2)

[0057] Where V is the volume of the cell fluid in the mixing temperature control device, α is the relationship coefficient between k and V, and d is the bias.

[0058] Substituting formula (2-2) into formula (2-1) yields:

[0059] v1=(αV-d)×(v2+v3+v4+…+v n+1 ); (2-3)

[0060] As can be seen from formula (2-3), when the packaging flow rates in all the packaging pipelines 3 have been preset, that is, the sum of the packaging flow rates in all the packaging pipelines 3 has been preset, the specific flow rate v1 and the volume V of the cell fluid in the mixing temperature control device 1 can be adjusted so that the sum of the packaging flow rates in all the packaging pipelines 3 remains at the preset value, and then the packaging flow rate in each packaging pipeline 3 is controlled to maintain the corresponding preset value, thereby strictly controlling the packaging time of the packaging bag in each packaging pipeline 3 within the preset time;

[0061] When only part of the filling flow rates in all the filling pipelines 3 are preset, the filling flow rates in the filling pipelines with preset filling flow rates can be adjusted to maintain the corresponding preset values ​​by adjusting the specific flow rate v1, the volume V of the cell fluid in the mixing temperature control device 1, and the filling flow rates in other filling pipelines without preset filling flow rates, thereby strictly controlling the filling time of the filling bags in these filling pipelines within the preset time.

[0062] This embodiment establishes a relationship between a specific flow rate, the volume of the cell fluid in the mixing temperature control device, and multiple filling pipelines. Based on this relationship, the preset parameters can be controlled by non-preset parameters, and multiple control schemes can be implemented with multiple parameters. Therefore, the continuity and uninterrupted operation of the preset filling flow rate can be guaranteed from multiple aspects, that is, the mixing stage and the filling stage can be carried out simultaneously and continuously, ensuring that the filling time can be strictly controlled within the preset time. Furthermore, the filling efficiency of the cell fluid can be improved by shortening the preset time, and the short-time filling of large-volume cell fluid can be achieved.

[0063] Reference Figure 2 In one embodiment, the specific flow rate is a flow rate at which the Reynolds number of the cell fluid in the mixing temperature control device 1 reaches a critical value of turbulence.

[0064] The Reynolds number, Re, is a dimensionless number that can be used to characterize fluid flow. Re = ρvd / μ, where ρ, v, and μ are the density, velocity, and viscosity of the fluid, respectively, and d is the characteristic length. For example, if the fluid is flowing through a circular pipe, d is the equivalent diameter of the pipe. The Reynolds number can be used to distinguish between laminar and turbulent flow and to determine the resistance encountered by an object moving through the fluid.

[0065] Turbulence occurs when the Reynolds number is large. When the Reynolds number is small, the influence of viscous force on the flow field is greater than the inertial force, the disturbance of flow velocity in the flow field will be attenuated by the viscous force, and the fluid flow is stable and laminar; when the Reynolds number is large, the influence of inertial force on the flow field is greater than the viscous force, the fluid flow is relatively unstable, and small changes in flow velocity are easy to develop and enhance, forming a disordered and irregular turbulent flow field.

[0066] This embodiment controls the specific flow rate to a flow rate that makes the Reynolds number of the cell fluid in the mixing temperature control device reach the turbulence critical value, so that the cell fluid in the mixing temperature control device can form turbulence, that is, the cell fluid flows irregularly, which can increase the mixing effect of various liquids in the cell fluid and ensure the concentration and activity balance of the cell fluid after it is packaged into each packaging bag.

[0067] Reference Figure 2 In one embodiment, before controlling the cell solution in the mixing temperature control device 1 to flow in the filling pipeline 3 at the filling flow rate, the steps may include:

[0068] When the volume of the cell fluid in the mixing temperature control device 1 reaches a volume threshold, the subpackaging pipeline 3 is controlled to open.

[0069] That is, the first peristaltic pump 8 is turned on first, and the cell fluid in the sample bag 7 is injected into the mixing temperature control device 1. When the capacity of the cell fluid in the mixing temperature control device 1 reaches the capacity threshold, the second peristaltic pump 9, the third peristaltic pump 10 and the fourth peristaltic pump 11 are controlled to be turned on, thereby opening the corresponding filling pipeline 3.

[0070] Setting a suitable capacity threshold and keeping the cell fluid in the mixing and temperature control device 1 at the capacity threshold level at all times can ensure that during the packaging process, the cell fluid flows out of the mixing and temperature control device 1 continuously and uninterruptedly, flows in each packaging pipeline 3 at a preset packaging flow rate, and is injected into the packaging bag 2, thereby strictly controlling the packaging time.

[0071] In this embodiment, the capacity threshold may be set to 0.5 L based on the industry's packaging bag specifications and packaging time requirements.

[0072] In addition, when the volume of the cell fluid in the mixing temperature control device 1 reaches the volume threshold, multiple packaging pipelines 3 can be controlled to open simultaneously or separately, which is not limited here, as long as the actual packaging needs can be met.

[0073] This embodiment sets a volume threshold of the cell fluid in the mixing and temperature control device, and can maintain the volume threshold through the inlet and outlet fluids in the mixing and temperature control device, thereby ensuring that there is sufficient cell fluid in the mixing and temperature control device at all times, so that the cell fluid can flow continuously and uninterruptedly in each packaging pipeline at each preset packaging flow rate and be injected into the packaging bag, maintaining the mixing stage and the packaging stage simultaneously and continuously, thereby strictly controlling the packaging time.

[0074] Reference Figure 2 In one embodiment, before controlling the cell solution in the mixing temperature control device 1 to flow in the filling pipeline 3 at the filling flow rate, the steps may include:

[0075] Control the sterile filter 5 to open and connect the filling pipeline 3 to the atmosphere;

[0076] The sterile filter 5 is arranged at the end of the subpackaging pipeline 3 .

[0077] This embodiment connects the sub-packaging pipeline to the atmosphere to maintain the pressure in the sub-packaging pipeline, so that the cell fluid in the mixing temperature control device can be pumped into the sub-packaging pipeline by the peristaltic pump. At the same time, a sterile filter is provided at the end of the sub-packaging pipeline to prevent impurities in the atmosphere from entering the sub-packaging pipeline and entering the sub-packaging bag along with the cell fluid, thereby affecting the quality of the cell fluid in the sub-packaging bag.

[0078] Reference Figure 2 In one embodiment, before controlling the cell solution in the mixing temperature control device 1 to flow in the filling pipeline 3 at the filling flow rate, the steps may include:

[0079] The internal pressure of the filling pipeline 3 is monitored in real time by the pressure sensor 6, and the internal pressure of the filling pipeline 3 is maintained in a negative pressure state by controlling the opening and closing of the sterile filter 5;

[0080] The sterile filter 5 is provided at the end of the sub-packaging pipeline 3 , and the pressure sensor 6 is connected to the end of the sub-packaging pipeline 3 via the sterile filter 5 .

[0081] This embodiment maintains the internal pressure of the sub-packaging pipeline in a negative pressure state, which helps to use a smaller peristaltic pump power to extract the cell solution from the mixing temperature control device to the sub-packaging pipeline. At the same time, by setting a sterile filter, it can prevent impurities in the atmosphere from entering the sub-packaging pipeline and entering the sub-packaging bag along with the cell solution, thereby affecting the quality of the cell solution in the sub-packaging bag.

[0082] Reference Figure 2 In one embodiment, the inner diameter of the output pipeline 4 of the mixing temperature control device 1 is the sum of the inner diameters of the multiple filling pipelines 3.

[0083] This embodiment sets the inner diameter of the output pipeline of the mixing temperature control device to the sum of the inner diameters of multiple packaging pipelines, which can meet the demand for outputting a large amount of cell fluid when multiple packaging pipelines are opened simultaneously and cell fluid is packaged at multiple packaging flow rates, and prevent the inner diameter of the output pipeline from being too small to withstand the suction pressure of multiple pipelines and cause an explosion accident.

[0084] The cell fluid packaging time control device provided in an embodiment of the present application is described below. The cell fluid packaging time control device described below and the cell fluid packaging time control method described above can be referenced to each other.

[0085] Figure 3 This is a schematic diagram of the structure of the cell fluid packaging time control device provided in the embodiment of the present application. Figure 3 , the embodiment of the present application provides a cell fluid packaging time control device, which may include:

[0086] The specific flow rate determination module 301 is used to control the cell fluid to be injected into the mixing temperature control device at a specific flow rate;

[0087] The packaging flow rate determination module 302 is used to determine the packaging flow rate of the cell solution according to the capacity of the packaging bag and the preset packaging time;

[0088] The subpackaging control module 303 is configured to control the cell fluid in the mixing and temperature control device to flow in the subpackaging pipeline at the subpackaging flow rate according to the specific flow rate and the volume of the cell fluid in the mixing and temperature control device;

[0089] The sub-packaging pipeline is a pipeline between the end of the output pipeline of the mixing temperature control device and the sub-packaging bag, and the sub-packaging bag is connected to the sub-packaging pipeline.

[0090] The cell fluid packaging time control device provided in this embodiment first controls the cell fluid to be injected into the mixing temperature control device at a specific flow rate, then determines the packaging flow rate of the cell fluid based on the capacity of the packaging bag and the preset packaging time, and finally controls the cell fluid in the mixing temperature control device to flow in the packaging pipeline at the packaging flow rate based on the specific flow rate and the capacity of the cell fluid in the mixing temperature control device. Since the packaging flow rate is determined according to the capacity of the packaging bag and the preset packaging time, the packaging bag is connected to the packaging pipeline. Therefore, the packaging flow rate is the flow rate that can meet the requirements for completing the packaging of the packaging bag within the preset packaging time. The packaging flow rate is controlled according to the specific flow rate and the volume of the cell fluid in the mixing temperature control device. The packaging flow rate can be maintained by adjusting the cell fluid source factors, that is, the specific flow rate and the volume of the cell fluid that has been mixed and can be used for packaging, thereby ensuring that the mixing stage and the packaging stage are carried out simultaneously and continuously during the packaging process, thereby ensuring that the packaging time can be strictly controlled within the preset time, and then the packaging efficiency of the cell fluid can be improved by shortening the preset time, and short-time packaging of large-volume cell fluid can be achieved. Practice has shown that under the same other packaging conditions, the packaging time of this application can be shortened by about 80% compared with the traditional cell fluid packaging method.

[0091] In one embodiment, the subpackaging control module 303 is specifically configured to:

[0092] According to the linear relationship between the sum of the subpackaging flow rates and the specific flow rate, the cell fluid in the mixing temperature control device is controlled to flow in the subpackaging pipeline at the subpackaging flow rate; wherein the sum of the subpackaging flow rates is the sum of the subpackaging flow rates of different subpackaging pipelines, and the linear relationship coefficient between the sum of the subpackaging flow rates and the specific flow rate is linearly related to the volume of the cell fluid in the mixing temperature control device.

[0093] In one embodiment, the specific flow rate is a flow rate at which the Reynolds number of the cell solution in the mixing and temperature control device reaches a critical value of turbulence.

[0094] In one embodiment, a sub-packaging pipeline control module (not shown) is further included, which is used to:

[0095] When the volume of the cell fluid in the mixing temperature control device reaches a volume threshold, the subpackaging pipeline is controlled to open.

[0096] In one embodiment, the invention further comprises a pressure control module (not shown in the figure) for:

[0097] The sterile filter is controlled to open and connect the sub-packaging pipeline to the atmosphere; the sterile filter is arranged at the end of the sub-packaging pipeline.

[0098] In one embodiment, the invention further comprises a pressure control module (not shown in the figure) for:

[0099] The internal pressure of the sub-packaging pipeline is monitored in real time by a pressure sensor, and the internal pressure of the sub-packaging pipeline is maintained in a negative pressure state by controlling the opening and closing of the sterile filter; the sterile filter is arranged at the end of the sub-packaging pipeline, and the pressure sensor is connected to the end of the sub-packaging pipeline via the sterile filter.

[0100] In one embodiment, the inner diameter of the output pipeline of the mixing temperature control device is the sum of the inner diameters of the multiple filling pipelines.

[0101] Figure 4 An example of a physical structure diagram of an electronic device is shown below. Figure 4 As shown, the electronic device may include: a processor 410, a communication interface 420, a memory 430, and a communication bus 440, wherein the processor 410, the communication interface 420, and the memory 430 communicate with each other via the communication bus 440. The processor 410 may call a computer program in the memory 430 to execute the steps of the cell solution dispensing time control method, for example, including:

[0102] Control the cell fluid to be injected into the mixing temperature control device at a specific flow rate;

[0103] Determine the cell solution dispensing flow rate based on the capacity of the dispensing bag and the preset dispensing time;

[0104] According to the specific flow rate and the volume of the cell fluid in the mixing and temperature control device, controlling the cell fluid in the mixing and temperature control device to flow in the filling pipeline at the filling flow rate;

[0105] The sub-packaging pipeline is a pipeline between the end of the output pipeline of the mixing temperature control device and the sub-packaging bag, and the sub-packaging bag is connected to the sub-packaging pipeline.

[0106] In addition, the logic instructions in the above-mentioned memory 430 can be implemented in the form of a software functional unit and can be stored in a computer-readable storage medium when sold or used as an independent product. Based on this understanding, the technical solution of the present application, or the part that contributes to the prior art or the part of the technical solution, can be embodied in the form of a software product, which is stored in a storage medium and includes several instructions for enabling a computer device (which can be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the method described in each embodiment of the present application. The aforementioned storage medium includes: various media that can store program codes, such as a USB flash drive, a mobile hard disk, a read-only memory (ROM), a random access memory (RAM), a magnetic disk or an optical disk.

[0107] On the other hand, embodiments of the present application further provide a computer program product, comprising a computer program, which may be stored on a non-transitory computer-readable storage medium. When the computer program is executed by a processor, the computer can perform the steps of the cell solution dispensing time control method provided in the above embodiments, for example, including:

[0108] Control the cell fluid to be injected into the mixing temperature control device at a specific flow rate;

[0109] Determine the cell solution dispensing flow rate based on the capacity of the dispensing bag and the preset dispensing time;

[0110] According to the specific flow rate and the volume of the cell fluid in the mixing and temperature control device, controlling the cell fluid in the mixing and temperature control device to flow in the filling pipeline at the filling flow rate;

[0111] The sub-packaging pipeline is a pipeline between the end of the output pipeline of the mixing temperature control device and the sub-packaging bag, and the sub-packaging bag is connected to the sub-packaging pipeline.

[0112] On the other hand, an embodiment of the present application further provides a processor-readable storage medium, wherein the processor-readable storage medium stores a computer program, wherein the computer program is configured to cause a processor to execute the steps of the methods provided in the above embodiments, for example, including:

[0113] Control the cell fluid to be injected into the mixing temperature control device at a specific flow rate;

[0114] Determine the cell solution dispensing flow rate based on the capacity of the dispensing bag and the preset dispensing time;

[0115] According to the specific flow rate and the volume of the cell fluid in the mixing and temperature control device, controlling the cell fluid in the mixing and temperature control device to flow in the filling pipeline at the filling flow rate;

[0116] The sub-packaging pipeline is a pipeline between the end of the output pipeline of the mixing temperature control device and the sub-packaging bag, and the sub-packaging bag is connected to the sub-packaging pipeline.

[0117] The processor-readable storage medium can be any available medium or data storage device that can be accessed by the processor, including but not limited to magnetic storage (such as floppy disks, hard disks, magnetic tapes, magneto-optical disks (MO)), optical storage (such as CDs, DVDs, BDs, HVDs, etc.), and semiconductor storage (such as ROMs, EPROMs, EEPROMs, non-volatile memories (NANDFLASH), solid-state drives (SSDs)), etc.

[0118] The device embodiments described above are merely illustrative. The units described as separate components may or may not be physically separate, and the components shown as units may or may not be physical units, i.e., they may be located in one location or distributed across multiple network units. Some or all of the modules may be selected based on actual needs to achieve the objectives of the present embodiment. Persons of ordinary skill in the art will be able to understand and implement the present invention without inventive effort.

[0119] Through the description of the above embodiments, those skilled in the art can clearly understand that each embodiment can be implemented by means of software plus a necessary general hardware platform, or of course, by hardware. Based on this understanding, the essence of the above technical solution or the part that contributes to the existing technology can be embodied in the form of a software product. The computer software product can be stored in a computer-readable storage medium, such as ROM / RAM, a magnetic disk, an optical disk, etc., and includes a number of instructions for enabling a computer device (which can be a personal computer, a server, or a network device, etc.) to execute the methods described in each embodiment or certain parts of the embodiments.

[0120] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for controlling cell fluid packaging time, characterized in that: include: Controlling the cell fluid to be injected into the mixing and temperature control device at a specific flow rate; the specific flow rate is the flow rate at which the Reynolds number of the cell fluid in the mixing and temperature control device reaches a critical value of turbulence; Determine the cell solution dispensing flow rate based on the capacity of the dispensing bag and the preset dispensing time; According to the specific flow rate and the volume of the cell fluid in the mixing and temperature control device, controlling the cell fluid in the mixing and temperature control device to flow in the filling pipeline at the filling flow rate includes: According to a linear relationship between the sum of the subpackaging flow rates and the specific flow rate, controlling the cell fluid in the mixing and temperature control device to flow in the subpackaging pipeline at the subpackaging flow rate; wherein the sum of the subpackaging flow rates is the sum of the subpackaging flow rates of different subpackaging pipelines, and the linear relationship coefficient between the sum of the subpackaging flow rates and the specific flow rate is linearly related to the volume of the cell fluid in the mixing and temperature control device; The sub-packaging pipeline is the pipeline between the end of the output pipeline of the mixing temperature control device and the sub-packaging bag, the sub-packaging bag is connected to the sub-packaging pipeline, and the inner diameter of the output pipeline of the mixing temperature control device is the sum of the inner diameters of multiple sub-packaging pipelines.

2. The method for controlling cell fluid dispensing time according to claim 1, wherein: Before controlling the cell fluid in the mixing temperature control device to flow in the filling pipeline at the filling flow rate, the method includes: When the volume of the cell fluid in the mixing temperature control device reaches a volume threshold, the subpackaging pipeline is controlled to open.

3. The method for controlling cell fluid dispensing time according to claim 1, wherein: Before controlling the cell fluid in the mixing temperature control device to flow in the filling pipeline at the filling flow rate, the method includes: The sterile filter is controlled to open and connect the sub-packaging pipeline to the atmosphere; the sterile filter is arranged at the end of the sub-packaging pipeline.

4. The method for controlling cell fluid dispensing time according to claim 1, wherein: Before controlling the cell fluid in the mixing temperature control device to flow in the filling pipeline at the filling flow rate, the method includes: The internal pressure of the sub-packaging pipeline is monitored in real time by a pressure sensor, and the internal pressure of the sub-packaging pipeline is maintained in a negative pressure state by controlling the opening and closing of the sterile filter; the sterile filter is arranged at the end of the sub-packaging pipeline, and the pressure sensor is connected to the end of the sub-packaging pipeline via the sterile filter.

5. A cell fluid dispensing time control device, characterized in that: The method for controlling the time of cell solution packaging according to claim 1 comprises: A specific flow rate determination module is used to control the cell fluid to be injected into the mixing temperature control device at a specific flow rate; The packaging flow rate determination module is used to determine the packaging flow rate of the cell solution according to the capacity of the packaging bag and the preset packaging time; A subpackaging control module is configured to control the cell fluid in the mixing and temperature control device to flow in the subpackaging pipeline at the subpackaging flow rate according to the specific flow rate and the volume of the cell fluid in the mixing and temperature control device; The sub-packaging pipeline is a pipeline between the end of the output pipeline of the mixing temperature control device and the sub-packaging bag, and the sub-packaging bag is connected to the sub-packaging pipeline.

6. An electronic device comprising a processor and a memory storing a computer program, characterized in that: When the processor executes the computer program, the steps of the cell fluid packaging time control method according to any one of claims 1 to 4 are implemented.

7. A non-transitory computer-readable storage medium having a computer program stored thereon, characterized in that: When the computer program is executed by a processor, the steps of the cell fluid packaging time control method according to any one of claims 1 to 4 are implemented.

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