A cell liquid dispensing apparatus

Abstract

The present application relates to the technical field of cell liquid dispensing, and provides a cell liquid dispensing device, which comprises a body, a dispensing device, a mixing device and a sample bag placing device, and a cavity is arranged in the body; the dispensing device comprises a dispensing panel and a dispensing component, the dispensing panel is arranged in the up-down direction and separates the cavity into a mixing refrigeration cavity and a dispensing cavity, and the dispensing component is arranged on the dispensing panel; the mixing device is arranged in the mixing refrigeration cavity; the sample bag placing device is arranged in the mixing refrigeration cavity and is connected with the mixing device; a temperature control device is arranged in the mixing refrigeration cavity, and the temperature control device is used for keeping the mixing refrigeration cavity and the dispensing cavity at a predetermined temperature. The cell liquid dispensing device integrates the mixing device, the sample bag placing device and the temperature control device in the mixing refrigeration cavity, and reasonably arranges the positional relationship of the above devices, so that the compactness of the dispensing device can be further improved, the volume of the cell liquid dispensing device can be reduced, and the cell liquid dispensing device is convenient to carry.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell liquid dispensing, and particularly to a cell liquid dispensing device. BACKGROUND

[0002] Dispensing preparation is an important link in cell preparation process, and the dispensing preparation is related to the uniformity of the volume and density of the preparation, and the cell recovery rate, survival rate and function before freezing. SUMMARY

[0003] The present application provides a cell liquid dispensing device to solve the problem of large space occupation and inconvenient transportation of the cell liquid dispensing device in the prior art due to large volume.

[0004] The present application provides a cell liquid dispensing device, comprising:

[0005] A machine body is internally provided with a cavity;

[0006] A dispensing device comprises a dispensing panel and a dispensing component, the dispensing panel is arranged in the up-down direction and separates the cavity into a uniform mixing refrigeration cavity and a dispensing cavity, and the dispensing component is arranged on the dispensing panel;

[0007] A uniform mixing device is arranged in the uniform mixing refrigeration cavity;

[0008] A sample bag placing device is arranged in the uniform mixing refrigeration cavity and connected with the uniform mixing device;

[0009] A temperature control device is arranged in the uniform mixing refrigeration cavity, and the temperature control device is used to make the uniform mixing refrigeration cavity and the dispensing cavity at a predetermined temperature.

[0010] According to the cell liquid dispensing device provided by the embodiment of the present application, the front end of the machine body is provided with a first opening communicated with the dispensing cavity, the first opening is provided with a first door body connected with the machine body, the first side wall of the machine body is provided with a second opening communicated with the uniform mixing refrigeration cavity, and the second opening is provided with a second door body connected with the machine body.

[0011] According to the cell liquid dispensing device provided by the embodiment of the present application, the sample bag placing device comprises:

[0012] A fixing seat is connected with the uniform mixing device;

[0013] A telescopic component, the first end of the telescopic component is connected with the fixing seat, and the telescopic component is adapted to switch between the elongated state and the shortened state;

[0014] A sample placing box is connected with the second end of the telescopic component, and the sample placing box is used for placing a sample bag; in the elongated state, the sample placing box is extended through the second opening; and in the shortened state, the sample placing box is located in the mixing and refrigerating cavity.

[0015] According to the cell liquid dispensing equipment provided in the embodiment of the present application, the sample bag placing device further comprises:

[0016] A bearing plate is downwardly and obliquely arranged above the sample placing box, and the sample bag is placed on the bearing plate.

[0017] A sample bag tensioning component is arranged below the sample placing box and connected with the machine body, the sample bag tensioning component is connected with the liquid outlet pipe of the sample bag, and the sample bag tensioning component is used for tensioning the liquid outlet pipe of the sample bag downwardly so that the liquid outlet pipe of the sample bag is below the sample bag.

[0018] According to the cell liquid dispensing equipment provided in the embodiment of the present application, the mixing device comprises:

[0019] A base component;

[0020] A cold plate is arranged on the upper portion of the base component, and the cold plate is arranged opposite to the second opening.

[0021] A mixing plate component is movably arranged on the side of the cold plate facing the second opening.

[0022] A driving component is arranged on the side of the cold plate away from the second opening and connected with the mixing plate component, and the driving component is used for driving the mixing plate component to press the mixing bag so that the liquid in the mixing bag is mixed uniformly.

[0023] According to the cell liquid dispensing equipment provided in the embodiment of the present application, the mixing plate component comprises:

[0024] A mixing plate, a first end of the mixing plate is hinged with the driving component.

[0025] A fixing component is connected with the driving component.

[0026] A locking component is arranged on the second end of the mixing plate, and the locking component is locked with the fixing component.

[0027] According to the cell liquid dispensing equipment provided in the embodiment of the present application, the temperature control device comprises:

[0028] The refrigeration component includes an evaporator, a condenser, a compressor, a capillary tube and a first fan, the evaporator is arranged on the side of the cold plate away from the second opening, the evaporator is in fluid communication with the condenser, the compressor and the capillary tube, the condenser and the compressor are arranged on the base component; the second side wall of the machine body is provided with an air outlet, and the first fan is arranged on one side of the condenser and arranged corresponding to the air outlet;

[0029] The temperature sensor is arranged on the side of the cold plate away from the second opening.

[0030] The temperature control device further includes:

[0031] The air supply component includes an air duct, at least one second fan and a heat sink, the air duct is arranged on the side of the cold plate away from the second opening, the air duct has an air inlet and an air outlet, the heat sink is arranged inside the air duct and is attached to the cold plate; at least one of the air inlet and the air outlet of the air duct is provided with the second fan, and the air inlet and the air outlet of the air duct are both communicated with the dispensing cavity through pipelines.

[0032] The dispensing device includes a first dispensing panel, a second dispensing panel and a third dispensing panel, the first dispensing panel is arranged on the upper part of the second dispensing panel, the second dispensing panel is arranged on the upper part of the third dispensing panel, the lower edges of the first dispensing panel and the second dispensing panel are both provided with a flow collecting groove, and the bottom of the machine body is provided with a drainage groove in communication with the flow collecting groove.

[0033] The dispensing component includes:

[0034] The flow detection assembly includes a main pipeline, a second pump body arranged on the main pipeline and a flow detection piece, and the flow detection piece is connected in series to the main pipeline and located in the downstream direction of the second pump body.

[0035] The conveying assembly includes a pipeline assembly, a first pump body, a first control valve and a second control valve arranged on the third dispensing panel, the pipeline assembly includes a first pipeline, a second pipeline and an air inlet pipeline, the first pipeline is respectively communicated with a sample bag and a mixing bag, and the first pump body is arranged on the first pipeline; the second pipeline is respectively communicated with the first pipeline and the air inlet pipeline, the first control valve is arranged on the second pipeline, and the second control valve is arranged on the air inlet pipeline.

[0036] Two sub-packaging assemblies, one of which is arranged on the first sub-packaging panel and the other of which is arranged on the second sub-packaging panel, the sub-packaging assembly comprising a plurality of sub-packaging control valves, a branch pipeline in communication with the main pipeline, and a plurality of sub-packaging pipelines in communication with the branch pipeline, the plurality of sub-packaging control valves being arranged in one-to-one correspondence with the plurality of sub-packaging pipelines, and the plurality of sub-packaging pipelines being in one-to-one correspondence with the preparation bags;

[0037] A controller electrically connected with the first pump body, the first control valve, the second control valve, the sub-packaging control valve, the second pump body, and the flow detection component, respectively.

[0038] According to the cell liquid sub-packaging equipment provided by the embodiment of the present application, the flow detection assembly further comprises:

[0039] A drip cup connected in series with the main pipeline and located between the second pump body and the first control valve;

[0040] A drip cup rotating component arranged on the third sub-packaging panel, the drip cup rotating component being connected with the drip cup, the drip cup rotating component being electrically connected with the controller, and the drip cup rotating component being used to drive the drip cup to rotate.

[0041] According to the cell liquid sub-packaging equipment provided by the embodiment of the present application, the flow detection component is a dosing tube, and the flow detection assembly further comprises:

[0042] A dosing tube rotating component arranged on the second sub-packaging panel, the dosing tube rotating component being connected with the dosing tube, the dosing tube rotating component being electrically connected with the controller, and the dosing tube rotating component being used to drive the dosing tube to rotate.

[0043] The cell liquid sub-packaging equipment provided by the embodiment of the present application has the advantages that: the cavities are divided into a uniform-mixing refrigeration cavity and a sub-packaging cavity by using the sub-packaging panel, the sub-packaging panel is used as a bearing component of the sub-packaging component, and a separate component is not needed to be arranged as a bearing component of the sub-packaging component, so that the compactness of the sub-packaging equipment is improved; the uniform-mixing device, the sample bag placing device, and the temperature control device are integrated in the uniform-mixing refrigeration cavity, and the positional relationship of the above devices is reasonably arranged, so that the compactness of the sub-packaging equipment is further improved, the volume of the cell liquid sub-packaging equipment is reduced, and the cell liquid sub-packaging equipment is convenient to carry. BRIEF DESCRIPTION OF DRAWINGS

[0044] In order to more clearly illustrate the technical solutions in the present application or prior art, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description. Obviously, the drawings in the following description are some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without any creative effort on the basis of these drawings.

[0045] Figure 1 is one of the structural schematic diagrams of the cell liquid dispensing equipment provided by the embodiment of the present application in the case that the second door body is in the open state;

[0046] Figure 2 is one of the structural schematic diagrams of the cell liquid dispensing equipment provided by the embodiment of the present application in the case that the second door body is in the open state;

[0047] Figure 3 is the exploded structural schematic diagram of the sample bag placing device provided by the embodiment of the present application;

[0048] Figure 4 is the structural schematic diagram of the temperature control device provided by the embodiment of the present application;

[0049] Figure 5 is the structural schematic diagram of the mixing device provided by the embodiment of the present application;

[0050] Figure 6 is the exploded structural schematic diagram of the mixing device provided by the embodiment of the present application;

[0051] Figure 7 is the structural schematic diagram of the dispensing assembly provided by the embodiment of the present application;

[0052] Figure 8 is the assembly relationship structural schematic diagram of the first pump body, the second pump body and the third dispensing panel provided by the embodiment of the present application;

[0053] Figure 9 is the assembly relationship structural schematic diagram of the quantitative tube rotating piece, the second bubble sensor, the third bubble sensor and the second dispensing panel provided by the embodiment of the present application;

[0054] Figure 10 is the assembly relationship structural schematic diagram of the first dispensing assembly and the first dispensing panel provided by the embodiment of the present application.

[0055] Reference signs:

[0056] 110, first pump body; 120, first control valve; 130, second control valve; 140, sample bag; 150, mixing bag; 160, first pipeline; 170, second pipeline; 180, air inlet pipeline; 210, second pump body; 220, flow detection member; 221, dosing tube rotating member; 230, drip cup; 231, drip cup rotating member; 240, first bubble sensor; 250, second bubble sensor; 260, third bubble sensor; 270, main pipeline; 310, air pressure sensor; 320, fourth bubble sensor; 341, first branch pipeline; 342, first dispensing control valve; 351, second branch pipeline; 352, second dispensing control valve; 410, first dispensing panel; 420, second dispensing panel; 430, third dispensing panel; 500, sample bag placing device; 510, fixed seat; 520, telescopic component; 530, sample placing box; 540, bearing plate; 560, sample bag tensioning component; 600, machine body; 630, first door body; 640, second door body; 650, preparation bag; 660, touch screen component; 670, start button; 700, mixing device; 710, base component; 720, cold plate; 730, mixing plate component; 731, mixing plate; 732, fixing member; 733, locking member; 740, driving component; 750, driving motor; 751, driving wheel; 752, driven wheel; 753, belt; 754, connecting block; 755, fixed base plate; 756, guide member; 757, guide rod; 758, connecting plate; 800, temperature control device; 810, evaporator; 820, compressor; 830, first fan; 840, air duct; 850, temperature sensor. DETAILED DESCRIPTION

[0057] The embodiments of the present application will be further described below in conjunction with the drawings and examples. The following examples are used to illustrate the present application, but cannot be used to limit the scope of the present application.

[0058] In the description of the embodiments of the present application, it should be noted that the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the embodiments of the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the embodiments of the present application. In addition, the terms "first", "second", "third" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0059] In the description of the embodiments of the present application, it should be noted that unless specifically defined and limited otherwise, the terms "connected", "connected to", "connection" should be interpreted broadly, for example, can be fixed connection, can also be detachable connection, or integral connection; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through an intermediate medium. For those skilled in the art, the specific meaning of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0060] In the embodiments of the present application, unless specifically defined and limited otherwise, the first feature is "on" or "under" the second feature can be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature "above", "over" and "on" the second feature can be that the first feature is directly above or obliquely above the second feature, or only indicates that the first feature is higher in horizontal height than the second feature. The first feature "below", "under" and "under" the second feature can be that the first feature is directly below or obliquely below the second feature, or only indicates that the first feature is lower in horizontal height than the second feature.

[0061] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In the present application, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, those skilled in the art can combine and combine different embodiments or examples described in the present application and the features of different embodiments or examples without contradiction.

[0062] The following will be described in conjunction with Figures 1-10 The cell liquid dispensing apparatus provided by the embodiments of the present application is described.

[0063] Figure 1 One of the structural schematic diagrams of the cell liquid dispensing apparatus provided by the embodiments of the present application in the second door body in the open state is illustrated, Figure 2 One of the structural schematic diagrams of the cell liquid dispensing apparatus provided by the embodiments of the present application in the second door body in the open state is illustrated, as Figure 1 and Figure 2As shown, the cell liquid dispensing device comprises a body, a dispensing device, a mixing device 700, a sample bag placing device 500 and a temperature control device 800, the body is used to provide a mounting base for the above devices, the body is internally provided with a cavity, the dispensing device comprises a dispensing panel and a dispensing component, the dispensing panel is arranged in the up-down direction and separates the cavity into a mixing refrigeration cavity and a dispensing cavity, wherein the dispensing cavity is located at the front side of the dispensing panel, and the dispensing cavity provides a place for dispensing the cell liquid. The mixing refrigeration cavity is located at the rear side of the dispensing panel, and the dispensing component is arranged on the dispensing panel. The mixing device 700 is arranged in the mixing refrigeration cavity, the sample bag placing device 500 is arranged in the mixing refrigeration cavity and connected with the mixing device 700. The temperature control device 800 is arranged in the mixing refrigeration cavity, and the temperature control device 800 is used to make the mixing refrigeration cavity and the dispensing cavity be at a predetermined temperature.

[0064] The cell liquid dispensing device provided by the embodiment of the present application separates the cavity into a mixing refrigeration cavity and a dispensing cavity by using a dispensing panel, the dispensing panel is used as a bearing component of the dispensing component, and a separate component is not needed to be arranged as a bearing component of the dispensing component, so that the compactness of the dispensing device is improved. By integrating the mixing device 700, the sample bag placing device 500 and the temperature control device 800 in the mixing refrigeration cavity and reasonably arranging the positional relationship of the above devices, the compactness of the dispensing device can be further improved, the volume of the cell liquid dispensing device is reduced, and the cell liquid dispensing device is convenient to carry.

[0065] In the embodiment of the present application, the body 600 is a cuboid, so that an opening can be arranged on the side wall and the front end of the body 600. The front end of the body 600 is provided with a first opening which is in communication with the dispensing cavity, and the first opening is a rectangular opening. The first opening is provided with a first door body 630 which is connected with the body 600. Specifically, the body 600 is hinged or snap-fit connected with the first door body 630. The first door body 630 is a rectangular door body, and the size of the first door body 630 is matched with the size of the first opening.

[0066] The first side wall of the body 600 is provided with a second opening which is in communication with the mixing refrigeration cavity, and the second opening is a rectangular opening. By arranging the second opening on the first side wall of the body 600, the sample placing box 530 can be extended from the first side wall, and the sample bag 140 and the preparation bag 650 can be taken without affecting each other, so that the use is more convenient. The second opening is provided with a second door body 640 which is connected with the body 600. Specifically, the body 600 is hinged or snap-fit connected with the first door body 630. The second door body 640 is a rectangular door body, and the size of the second door body 640 is matched with the size of the first opening.

[0067] As Figure 1As shown, the first door body 630 and the second door body 640 are both transparent materials, and specifically, the materials of the first door body 630 and the second door body 640 can be glass or transparent plastic. The door bodies made of transparent materials can facilitate observation of the mixing state of the mixing bag 150 and the dispensing state of the preparation bag 650.

[0068] In the embodiments of the present application, Figure 3 An explosion structure schematic diagram of the sample bag placing device provided by the embodiments of the present application is shown in Figure 3 As shown, the sample bag placing device 500 includes a fixed seat 510, a telescopic component 520, and a sample placing box 530. The fixed seat 510 is connected with the mixing device 700, and specifically, the fixed seat 510 is connected with the top of the cold plate 720 through a screw. The telescopic component 520 is horizontally arranged, the first end of the telescopic component 520 is connected with the fixed seat 510, and the telescopic component 520 is adapted to switch between an elongated state and a shortened state. By using the telescopic component 520, the space occupied by the sample bag placing device 500 when it is accommodated in the mixing and refrigeration cavity can be effectively reduced, and the volume of the cell liquid dispensing equipment can be further reduced.

[0069] The sample placing box 530 is connected with the second end of the telescopic component 520, and the sample placing box 530 is used for placing the sample bag 140. In the elongated state, the sample placing box 530 extends out through the second opening to facilitate the user to take or place the sample bag 140. In the shortened state, the sample placing box 530 is located in the mixing and refrigeration cavity to reduce the volume of the sample bag placing device 500. In the present embodiment, the sample placing box 530 can place a sample bag 140 with a volume of 5L or less.

[0070] In the embodiments of the present application, the telescopic component 520 includes two fixed rails and two sliding rails. The two fixed rails are arranged in parallel and at intervals, the first ends of the two fixed rails are connected with the fixed seat 510 through screws, and the second ends of the two fixed rails extend towards the second opening. A sliding groove is arranged on the fixed rail and extends along the length direction of the fixed rail, and the two sliding rails are correspondingly clamped in the two sliding grooves and are in sliding fit with the corresponding fixed rails.

[0071] During use, the sample placing box 530 is pulled to make the sliding rails slide out of the sliding grooves, the length of the telescopic component 520 is elongated, the sample placing box 530 extends out of the second opening to facilitate the user to take and place the sample bag 140, and after use, the sample placing box 530 is pushed inward, the sliding rails slide into the sliding grooves, the total length of the telescopic component 520 is shortened, and the sample placing box 530 is accommodated in the mixing and refrigeration cavity.

[0072] It should be noted that the structure of the telescopic component 520 is not limited to the structure of the sliding rail, and can also be the structure of a telescopic rod assembly.

[0073] In the embodiment of the present application, the sample bag placing device 500 further comprises a bearing plate 540 and a weight sensor (not shown), the bearing plate 540 is a rectangular plate body, the bearing plate 540 is downwardly inclined and arranged above the sample placing box 530 towards the first opening, and the sample bag 140 is placed on the upper part of the bearing plate 540. By placing the bearing plate 540 downwardly inclined, the cell liquid in the sample bag 140 can be completely discharged by gravity. The weight sensor is arranged between the bearing plate 540 and the sample placing box 530, and is connected with the bottom of the bearing plate 540 and the upper part of the sample placing box 530. The weight sensor is electrically connected with the controller, and is used to detect the weight of the sample bag 140 in real time, so as to accurately control the amount of liquid output by the sample bag 140.

[0074] In the embodiment of the present application, the sample bag placing device 500 further comprises a sample bag tensioning component 560, which is arranged below the sample placing box 530 and connected with the machine body 600, and is connected with the liquid outlet pipe of the sample bag 140. The sample bag tensioning component 560 is used to tension the liquid outlet pipe of the sample bag 140 downwardly, so that the liquid outlet pipe of the sample bag 140 is below the sample bag 140.

[0075] The sample bag tensioning component 560 comprises a shell, a tension spring and a telescopic rod. The inside of the shell is hollow, the first end of the shell is provided with an outlet, the tension spring is arranged in the inside of the shell, and the first end of the tension spring is connected with the inner wall of the shell. The first end of the telescopic rod is slidably clamped in the outlet, the first end of the telescopic rod is connected with the second end of the tension spring, and the second end of the telescopic rod is connected with the liquid outlet pipe of the sample bag 140.

[0076] In use, the second end of the telescopic rod is connected with the liquid outlet pipe of the sample bag 140, the tension spring is ensured to be in a tension state, the second end of the telescopic rod is driven by the tension spring to enter the inside of the shell, and under the action of the tension of the tension spring, the liquid outlet pipe of the sample bag 140 is tensioned downwardly, so that the liquid outlet pipe of the sample bag 140 is entirely below the sample bag 140.

[0077] In order to conveniently adjust the tension of the liquid outlet pipe, the telescopic rod is provided with a plurality of connecting rods, the connecting rods are arranged at intervals along the length direction of the telescopic rod, and the distance between adjacent two connecting rods is equal. In use, by connecting the liquid outlet pipe with different connecting rods, the tension of the liquid outlet pipe can be adjusted as required, and the liquid outlet pipe of the sample bag 140 is ensured to be entirely below the sample bag 140.

[0078] In the embodiment of the present application, Figure 5 The structure schematic diagram of the mixing device provided by the embodiment of the present application is shown, Figure 6 The explosion structure schematic diagram of the mixing device provided by the embodiment of the present application is shown,Figure 5 and Figure 6 As shown in FIGS. 7A and 7B, the mixing device 700 comprises a base member 710, a cold plate 720, a sample mixing plate member 730, and a driving member 740. The base member 710 is used to provide a mounting base for the cold plate 720. The cold plate 720 is a rectangular metal plate. The cold plate 720 is a metal plate with excellent heat conduction performance. The cold plate 720 is vertically arranged on the upper portion of the base member 710. The cold plate 720 is arranged opposite to the second opening. The top portion of the cold plate 720 is provided with a hook. The hook is used to hang the mixing bag 150.

[0079] The sample mixing plate member 730 is movably arranged on the side of the cold plate 720 facing the second opening. The sample mixing plate member 730 is arranged in parallel with the cold plate 720. In this embodiment, the sample mixing plate member 730 is provided with one. Of course, the sample mixing plate member 730 can also be provided with two. When the sample mixing plate member 730 is provided with two, the two sample mixing plate members 730 are arranged in an upper and lower spaced manner. The driving member 740 is arranged on the side of the cold plate 720 away from the second opening. The driving member 740 is connected with the sample mixing plate member 730. The driving member 740 is used to drive the sample mixing plate member 730 to press the mixing bag 150, so that the liquid in the mixing bag 150 is uniformly mixed. Specifically, the driving member 740 drives the sample mixing plate member 730 to move close to and away from the cold plate 720, thereby pressing the mixing bag 150.

[0080] In the embodiment of the present application, as shown in FIGS. 7A and 7B, the driving member 740 comprises a fixed base plate 755, a driving assembly, and a guide assembly. The fixed base plate 755 is arranged on the side of the cold plate 720 away from the second opening. The fixed base plate 755 is connected with the cold plate 720 by bolts. Arranging the fixed base plate 755 on the side of the cold plate 720 away from the second opening can avoid the mixing bag 150 from contacting the driving assembly, thereby preventing the mixing bag 150 from being damaged. The driving assembly is arranged on the fixed base plate 755. The guide assembly is connected with the driving assembly, the cold plate 720, and the mixing plate 731, respectively. Figure 5 Figure 6 In the embodiment of the present application, as shown in FIGS. 7A and 7B, the driving member 740 comprises a fixed base plate 755, a driving assembly, and a guide assembly. The fixed base plate 755 is arranged on the side of the cold plate 720 away from the second opening. The fixed base plate 755 is connected with the cold plate 720 by bolts. Arranging the fixed base plate 755 on the side of the cold plate 720 away from the second opening can avoid the mixing bag 150 from contacting the driving assembly, thereby preventing the mixing bag 150 from being damaged. The driving assembly is arranged on the fixed base plate 755. The guide assembly is connected with the driving assembly, the cold plate 720, and the mixing plate 731, respectively.

[0081] The driving assembly comprises a driving motor 750, a driving wheel 751, a driven wheel 752, a belt 753, and a connecting block 754. The driving motor 750 is arranged on one end of the fixed base plate 755 close to the cold plate 720. The driving motor 750 is electrically connected with the controller. The driving wheel 751 is connected with the rotating shaft of the driving motor 750. The driven wheel 752 is rotatably arranged on the other end of the fixed base plate 755 away from the cold plate 720. The driven wheel 752 is connected with the driving wheel 751 by the belt 753. The connecting block 754 is connected with the belt 753. Specifically, the connecting block 754 comprises a first connecting portion and a second connecting portion. The first connecting portion and the second connecting portion are connected by screws. The belt 753 is clamped between the first connecting portion and the second connecting portion. ​

[0082] The guiding assembly is provided with two groups, each group of guiding assembly comprising two guides 756, two guide rods 757 and a connecting plate 758, the cold plate 720 is provided with a through hole, and the guide 756 is embedded in the through hole. The guide 756 is used to provide guidance for the guide rod 757 to reduce the resistance during the movement of the guide rod 757, and the guide 756 can be a linear bearing or a sliding sleeve. The two guides 756 correspond to the two guide rods 757 one by one.

[0083] The two guide rods 757 are horizontally arranged, and the two guide rods 757 are arranged in an upper and lower spaced manner. The guide rod 757 extends along the thickness direction of the cold plate 720, and the guide rod 757 is arranged in the corresponding guide 756 and is in sliding fit with the guide 756. The first ends of the two guide rods 757 of the same group are connected with the sample mixing plate component 730 respectively, and the second ends of the two guide rods 757 of the same group are connected with the same end of the connecting plate 758. The connecting plate 758 is connected with the connecting block 754 through screws.

[0084] When the driving motor 750 reciprocating rotates, the driving motor 750 drives the belt 753 to reciprocate through the driving wheel 751, the belt 753 drives the connecting block 754 to approach and move away from the cold plate 720, and the connecting block 754 drives the sample mixing plate component 730 to approach and move away from the cold plate 720 through the guiding assembly. The driving motor 750 is preferably a servo motor, and the servo motor can adapt to different concentrations of cell mixing due to the wide frequency adjustment range.

[0085] In the embodiment of the application, as shown in Figure 4 The base component 710 comprises a base and a guide rail, the guide rail is arranged on the bottom wall of the mixing refrigeration cavity, the guide rail extends towards the second opening, and the base is in sliding fit with the guide rail. The cold plate 720, the condenser and the compressor 820 are arranged on the base. By arranging the guide rail, the cold plate 720 and the refrigeration assembly component can move towards the second opening, which is convenient for installation and maintenance.

[0086] In the embodiment of the application, as shown in Figure 5 and Figure 6 The sample mixing plate component 730 comprises a mixing plate 731, a fixing piece 732 and a locking piece 733, the mixing plate 731 is a rectangular plate body, the mixing plate 731 is arranged in parallel with the cold plate 720, and the first end of the mixing plate 731 is hinged with the driving component 740, that is, the first end of the mixing plate 731 is connected with the first ends of the two guide rods of one group. The fixing piece 732 is connected with the driving component 740, that is, the fixing piece 732 is connected with the first ends of the two guide rods of the other group.

[0087] The locking member 733 is arranged at the second end of the mixing plate 731, and is locked with the fixing member 732. When the mixing bag 150 needs to be installed, the locking member 733 is opened, so that the mixing plate 731 is separated from the fixing member 732. Since the first end of the mixing plate 731 is connected with the first end of the two guide rods of one group, the mixing plate 731 can be rotated to open, so as to facilitate the installation and dismounting of the mixing bag 150. After the installation of the mixing bag 150 is completed, the mixing plate 731 is reversely rotated, and after the mixing plate 731 is rotated to the accurate position, the fixing of the mixing plate 731 is realized through the locking of the locking member 733 with the fixing member 732.

[0088] In the embodiment of the present application, Figure 4 The structural schematic diagram of the temperature control device provided by the embodiment of the present application is shown in the figure, Figure 4 As shown in the figure, the temperature control device 800 comprises a refrigeration component, the refrigeration component comprises an evaporator 810, a condenser, a compressor 820, a capillary tube and a first fan 830, the evaporator 810 is arranged on the side of the cold plate 720 away from the second opening, and the evaporator 810 is closely attached to the cold plate 720, so that the cold energy generated by the evaporator 810 is transmitted to the cold plate 720, and the temperature of the cold plate 720 starts to slowly drop to 1-25℃. The evaporator 810 is in fluid communication with the condenser, the compressor 820 and the capillary tube, the condenser and the compressor 820 are arranged on the base component 710; the second side wall of the machine body 600 is provided with an air outlet, and the first fan 830 is arranged on one side of the condenser and is correspondingly arranged with the air outlet. When the first fan 830 rotates, the heat generated by the condenser can be driven by the first fan 830 to flow out through the air outlet, so as to avoid the influence of the heat generated by the condenser on the refrigeration effect of the evaporator 810.

[0089] In the embodiment of the present application, the temperature control device 800 further comprises an air supply component, the air supply component comprises an air duct 840, at least one second fan and a heat sink, the air duct 840 is arranged on the side of the cold plate 720 away from the second opening, the air duct 840 has an air inlet and an air outlet, the heat sink is arranged in the air duct 840, and the heat sink is attached to the cold plate 720. In order to improve the heat dissipation effect, the heat sink is preferably a heat dissipation fin with a larger contact surface, the cold energy of the cold plate 720 is introduced into the air duct 840 through the heat dissipation fin, and then the air in the air duct 840 is cooled. At least one of the air inlet and the air outlet of the air duct 840 is provided with a second fan, and the air inlet and the air outlet of the air duct 840 are both in communication with the sub-packaging cavity through pipelines. In this embodiment, two air supply components are arranged, and the air ducts 840 of the two air supply components share the same pipeline in communication with the sub-packaging cavity. Alternatively, the air ducts 840 of the two air supply components are respectively in communication with the sub-packaging cavity through two pipelines. Arranging the second fan in the air duct 840 can improve the circulation efficiency of the cold air and reduce the noise.

[0090] When the second fan rotates, the second fan drives the air in the air duct 840 to flow, the air contacts the cooling fins, the temperature of the air is reduced, and the air with reduced temperature enters the sub-packaging cavity through the pipeline, so that the sub-packaging cavity meets the refrigeration requirement.

[0091] In the embodiment of the present application, the temperature control device 800 further comprises a temperature sensor 850, the temperature sensor 850 is arranged on the side of the cold plate 720 away from the second opening, the temperature sensor 850 is connected with the controller, the temperature sensor 850 is used to acquire the temperature of the cold plate 720, and the temperature of the cold plate 720 is compared with the target temperature to control the refrigeration component, so as to accurately control the temperature of the mixing and refrigeration cavity, reduce the functional consumption of the equipment, and prolong the service life of the equipment.

[0092] In the embodiment of the present application, the sub-packaging device comprises a first sub-packaging panel 410, a second sub-packaging panel 420 and a third sub-packaging panel 430, the first sub-packaging panel 410 is arranged on the upper part of the second sub-packaging panel, the second sub-packaging panel 420 is arranged on the upper part of the third sub-packaging panel 430, the lower edges of the first sub-packaging panel 410 and the second sub-packaging panel 420 are provided with flow collecting grooves, and the bottom of the machine body 600 is provided with a flow guiding groove in communication with the flow collecting grooves. By arranging the flow guiding groove and the flow collecting groove, the situation that there is condensed water or liquid leakage in the equipment can be prevented.

[0093] Figure 7 The structure schematic diagram of the sub-packaging assembly provided by the embodiment of the present application is shown in the figure, Figure 7 As shown in the figure, the sub-packaging component comprises a conveying assembly, two sub-packaging assemblies, a flow detection assembly and a controller. The flow detection assembly comprises a second pump body 210, a flow detection piece 220 and a main pipeline 270, the second pump body 210 is located in the third sub-packaging panel 430, and the second pump body 210 is arranged on the main pipeline 270. Specifically, the second pump body 210 is clamped on the main pipeline 270. The flow detection piece 220 is connected in series with the main pipeline 270 and located in the downstream direction of the second pump body 210.

[0094] The delivery assembly comprises a first pump body 110, a first control valve 120, a second control valve 130 and a pipeline assembly, the pipeline assembly comprises a first pipeline 160, a second pipeline 170 and an air inlet pipeline 180, the first pump body 110 is fixed to the third dispensing panel 430, the first pump body 110 is arranged in the first pipeline 160, specifically, the first pump body 110 is clamped in the first pipeline 160. The first pipeline 160 is in communication with the sample bag 140 and the mixing bag 150 respectively, under the driving of the first pump body 110, the sample liquid in the sample bag 140 enters the first pipeline 160, and the liquid in the mixing bag 150 enters the first pipeline 160 under the action of its own gravity. The second pipeline 170 is in communication with the first pipeline 160 and the air inlet pipeline 180 respectively, the first control valve 120 is arranged in the second pipeline 170, the sample liquid in the sample bag 140 and the liquid in the mixing bag 150 are mixed in the second pipeline 170 to obtain the cell liquid, the second control valve 130 is arranged in the air inlet pipeline 180, in this embodiment, the first control valve 120 is clamped in the second pipeline 170, and the second control valve 130 is clamped in the air inlet pipeline 180. In this embodiment, the first control valve 120 and the second control valve 130 are arranged on the third dispensing panel 430.

[0095] Two dispensing assemblies, one dispensing assembly is arranged on the first dispensing panel 410, and the other dispensing assembly is arranged on the second dispensing panel 420. The dispensing assembly comprises a plurality of dispensing control valves, a branch pipeline in communication with the main pipeline 270 and a plurality of dispensing pipes in communication with the branch pipelines, the plurality of dispensing control valves are arranged in one-to-one correspondence with the plurality of dispensing pipes, specifically, the plurality of dispensing control valves are clamped in one-to-one correspondence with the plurality of dispensing pipes, and the plurality of dispensing pipes are in one-to-one correspondence with the preparation bags 650.

[0096] According to the dispensing component provided by the embodiment of the application, the controller controls the first pump body 110 to work, drives the sample liquid in the sample bag 140 to enter the pipeline between the sample bag 140 and the first control valve 120, and the sample liquid and the liquid in the mixing bag 150 are mixed in the pipeline upstream of the first control valve 120 to obtain the cell liquid; the controller controls the plurality of dispensing control valves of each dispensing assembly to be opened in a predetermined order for a first predetermined time in sequence, so that the residual capacity of the preparation bag 650 corresponding to each dispensing assembly is equal to the capacity of the cell liquid in the corresponding pipeline; and the controller controls the dispensing control valves corresponding to the preparation bags 650 not filled with the cell liquid to be opened in sequence for a second predetermined time, so that the cell liquid in the corresponding pipeline is filled into the corresponding preparation bag 650 not filled with the cell liquid, and the cell liquid remaining in the pipeline is ensured to enter the corresponding preparation bag 650 completely, thereby avoiding waste of the cell liquid, improving the dispensing precision and the dispensing efficiency of cell dispensing, further improving the survival rate of cells, and avoiding adverse effects on the function of cells in the dispensing process.

[0097] In the embodiment of the present application, the air inlet pipeline 180 is in communication with the main pipeline 270, and an end of the air inlet pipeline 180 away from the second pipeline 170 is in communication with the external atmosphere. When the second control valve 130 in the air inlet pipeline 180 is in an open state, the external air can be pumped into the air inlet pipeline 180 and the main pipeline 270 under the action of the second pump body 210, so as to push the cell solution inside the main pipeline 270 to flow to the preparation bag 650.

[0098] In the embodiment of the present application, Figure 10 The assembly relationship structure diagram of the first sub-packaging assembly and the first sub-packaging panel provided by the embodiment of the present application is shown in Figure 7 and Figure 10 As shown in the figures, the sub-packaging components include a first sub-packaging assembly and a second sub-packaging assembly. The first sub-packaging assembly is arranged on the first sub-packaging panel 410, and includes a first branch pipeline 341, a plurality of first sub-packaging control valves 342, and a plurality of first sub-packaging pipelines in communication with the first branch pipeline 341. The plurality of first sub-packaging control valves 342 are arranged one by one in the plurality of first sub-packaging pipelines. When the cell solution fills the main pipeline 270 and the branch pipeline, i.e., when the cell solution fills the main pipeline 270 and the first branch pipeline 341, each first sub-packaging control valve 342 is controlled to be opened for a first predetermined time in a predetermined order (in this embodiment, the predetermined order is from left to right in Figure 7 ), so that the cell solution is sub-packaged into each preparation bag 650 corresponding to the first sub-packaging control valve 342 in the predetermined order. After the first predetermined time, the first sub-packaging control valve 342 is closed.

[0099] It should be noted that when the preparation bags 650 in the first branch pipeline 341 are sub-packaged, one or more first sub-packaging control valves 342 located at an end of the first branch pipeline 341 away from the main pipeline are in a closed state. At this time, the preparation bags 650 corresponding to the one or more first sub-packaging control valves 342 located at the end of the first branch pipeline 341 away from the main pipeline are not involved in the sub-packaging or the above preparation bags 650 only sub-packaged a part of the capacity. The predetermined order is not limited to the order from left to right in Figure 7 , but can also be the order from right to left in Figure 7 .

[0100] The second sub-packaging assembly includes a second branch pipeline 351, a plurality of second sub-packaging control valves 352, and a plurality of second sub-packaging pipelines in communication with the second branch pipeline 351. The plurality of second sub-packaging control valves 352 are arranged one by one in the plurality of second sub-packaging pipelines. After the sub-packaging of the preparation bags 650 corresponding to the first branch pipeline 341 is completed, each second sub-packaging control valve 352 is controlled to be opened for a second predetermined time in a predetermined order (in this embodiment, the predetermined order is from left to right in Figure 7In order from left to right, each second sub-packaging control valve 352 is controlled to open for a first predetermined time in turn, so that the cell solution is sub-packaged into the corresponding preparation bag 650 in a predetermined order, and the second sub-packaging control valve 352 is closed after the first predetermined time. The second sub-packaging assembly and the dosing tube rotating piece 221 are arranged on the second sub-packaging panel 420.

[0101] It should be noted that when the preparation bag 650 on the second branch pipeline 351 is sub-packaged, one or more second sub-packaging control valves 352 at the end of the second branch pipeline 351 away from the main pipeline 270 are in a closed state, and the corresponding preparation bag 650 of the one or more second sub-packaging control valves 352 at the end of the second branch pipeline 351 away from the main pipeline 270 does not participate in the sub-packaging.

[0102] It should also be noted that the first predetermined time of the first sub-packaging control valve 342 or the second sub-packaging control valve 352 can be the same or different, which is determined by the volume of the corresponding sub-packaged preparation bag 650 and the flow rate of the cell solution in the main pipeline 270.

[0103] When the sub-packaging of the preparation bag 650 corresponding to the second branch pipeline 351 is completed, the first control valve 120 is controlled to be closed, the second control valve 130 is controlled to be opened, and the second pump body 210 is started by the controller. Under the drive of the second pump body 210, air begins to enter the main pipeline 270 and push the residual cell solution in the main pipeline 270 into the branch pipeline, achieving emptying of the main pipeline 270. When the emptying of the main pipeline 270 is completed, one or more first sub-packaging control valves 342 at the end of the first branch pipeline 341 away from the main pipeline 270 are controlled to open for a second predetermined time in turn (the first sub-packaging control valve 342 is closed after the second predetermined time), so that the residual cell solution in the main pipeline 270 and the first branch pipeline 341 is sub-packaged into the corresponding preparation bag 650 of the one or more first sub-packaging control valves 342 at the end of the first branch pipeline 341 away from the main pipeline 270.

[0104] The first pump body 110 and the second pump body 210 are pump bodies with different displacements. Using pump bodies with different displacements for sub-packaging can adapt to different volumes of preparation bags for sub-packaging, improving the sub-packaging precision and efficiency.

[0105] After the cell liquid in the main pipe 270 and the first branch pipe 341 is exhausted, one or more second dispensing control valves 352 at the end of the second branch pipe 351 away from the main pipe 270 are opened in sequence for a second predetermined time, so that the cell liquid in the second branch pipe 351 is dispensed into the corresponding preparation bag 650. By exhausting the cell liquid in the main pipe 270 and the branch pipe, the waste of the cell liquid in the main pipe 270 and the branch pipe is effectively reduced.

[0106] It should be noted that the volume of the cell liquid not dispensed in the main pipe 270 and the first branch pipe 341 is equal to the remaining volume of the preparation bag 650 corresponding to the first branch pipe 341, and the volume of the cell liquid not dispensed in the second branch pipe 351 is equal to the volume of the preparation bag 650 corresponding to the second branch pipe 351. By controlling the dispensing control valve corresponding to each preparation bag 650 not filled with cell liquid to be opened for a second predetermined time in sequence, the cell liquid in the corresponding pipe is dispensed into the corresponding preparation bag 650 not filled with cell liquid, so that the cell liquid remaining in the pipe is completely entered into the corresponding preparation bag 650, the waste of the cell liquid is avoided, and the dispensing accuracy and efficiency of the cell dispensing are improved.

[0107] It should be further noted that the second predetermined time corresponding to the first dispensing control valve 342 or the second dispensing control valve 352 can be the same or different, which is determined by the volume of the preparation bag 650 not filled with cell liquid and the flow rate of the cell liquid in the main pipe 270 during the exhausting.

[0108] In the embodiment of the present application, as shown in Figure 7 The flow detection assembly includes a drip cup 230, which is connected in series to the main pipe 270 and located between the second pump body 210 and the first control valve 120.

[0109] In the embodiment of the present application, Figure 8 The assembly relationship structure of the first pump body, the second pump body and the third dispensing panel provided by the embodiment of the present application is illustrated, as shown in Figure 7 and Figure 8 The flow detection assembly further includes a drip cup rotating member 231, which is arranged on the third dispensing panel 430. The drip cup rotating member 231 is a motor or a combination of a motor and a speed reducer, and is preferably a servo motor, so as to accurately control the rotation angle of the motor. The drip cup rotating member 231 is connected with the drip cup 230. Specifically, the rotating shaft of the motor is provided with a fixing member, and the drip cup 230 is clamped on the fixing member. The drip cup rotating member 231 is connected with the controller, and is used to drive the drip cup 230 to rotate.

[0110] When the second control valve 130 is opened, the main pipeline 270 starts to be emptied, and the controller starts timing, when the second control valve 130 is opened for a third predetermined time, the dropper rotating piece 231 is rotated by the controller control, the dropper rotating piece 231 drives the dropper 230 to rotate 180° at a first predetermined speed, and the rotation speed of the dropper 230 is determined by the controller. By controlling the rotation of the dropper 230, it is convenient to empty the cell liquid remaining in the dropper 230, thereby reducing the waste of cell liquid in the main pipeline 270. In the embodiment, the third predetermined time is the time for emptying the cell liquid in the main pipeline 270 between the liquid inlet of the dropper 230 and the second connecting port of the first control valve 120, and one third of the cell liquid capacity of the dropper 230.

[0111] It should be noted that since the flow rate of the cell liquid in the pipeline is constant, the cell liquid capacity in the main pipeline 270 is also known, and the third predetermined time can be calculated according to the flow rate and the capacity of one third of the cell liquid in the dropper 230. The third predetermined time can be set according to the actual use requirement. The first predetermined speed is determined according to the type of cell liquid, and the first predetermined speed is preferably slow to reduce the shear force of the cells and avoid damaging the activity of the cells.

[0112] In the embodiment of the application, Figure 9 The assembly relationship structure schematic diagram of the quantitative pipe rotating piece, the second bubble sensor, the third bubble sensor and the second split panel provided by the embodiment of the application is shown as follows, Figures 7-9 The flow detection assembly further includes a first bubble sensor 240 and a second bubble sensor 250. The first bubble sensor 240 is arranged on the third split panel 430, the first bubble sensor 240 is arranged on the first pipeline 160, and is located between the first pump body 110 and the mixing bag 150. In the embodiment, the first bubble sensor 240 is clamped on the first pipeline 160. After the liquid in the mixing bag 150 enters the first pipeline 160, it passes through the first bubble sensor 240 and enters the second pipeline 170. The first bubble sensor 240 is used to detect whether the cell liquid flows through the first pipeline 160 in real time. If the first bubble sensor 240 does not detect that the cell liquid flows through, at this time, the liquid in the mixing bag 150 has been completely transported into the second pipeline 170.

[0113] The second bubble sensor 250 is arranged on the second dispensing panel 420, and is arranged on the main pipeline 270 and located between the flow detection member 220 and the second pump body 210. In this embodiment, the second bubble sensor 250 is clamped on the main pipeline 270, and the second bubble sensor 250 is used to detect whether the cell liquid flows through the main pipeline 270 between the outlet of the second pump body 210 and the liquid inlet of the flow detection member 220 in real time. If the second bubble sensor 250 detects that bubbles exist in the cell liquid flowing through the second bubble sensor 250, at this time, the cell liquid between the second bubble sensor 250 and the first control valve 120 has been emptied. The first bubble sensor 240 and the second bubble sensor 250 are electrically connected with the controller, so that the controller can obtain the detection information of the first bubble sensor 240 and the second bubble sensor 250, and record the time when the first bubble sensor 240 and the second bubble sensor 250 start to detect that no cell liquid flows through.

[0114] In the embodiment of the present application, the flow detection member 220 is a flow tube, which is used to detect the flow rate of the cell liquid in the main pipeline 270. The flow detection assembly further comprises a flow tube rotating member 221, which is arranged on the second dispensing panel 420 and connected with the flow tube. The flow tube rotating member 221 is an electric motor or a combination of an electric motor and a speed reducer, and is preferably a servo motor. The flow tube is connected with the rotating shaft of the electric motor, and the flow tube rotating member 221 is electrically connected with the controller and used to drive the flow tube to rotate.

[0115] When the second bubble sensor 250 detects that no cell liquid flows through the main pipeline 270, that is, most of the cell liquid in the main pipeline 270 has been transferred to the branch pipeline, the flow tube is driven to rotate by the flow tube rotating member 221, so as to facilitate the emptying of the cell liquid remaining in the flow tube and further reduce the waste of the cell liquid in the main pipeline 270.

[0116] In the embodiment of the present application, the flow detection assembly further comprises a third bubble sensor 260, which is electrically connected with the controller and arranged on the second dispensing panel 420. The third bubble sensor 260 is connected in series with the main pipeline 270 and located between the flow detection member 220 and the branch pipeline. In this embodiment, the third bubble sensor 260 is clamped on the main pipeline 270, and the third bubble sensor 260 is used to detect whether the cell liquid flows through the main pipeline 270 between the liquid outlet of the flow detection member 220 and the branch pipeline.

[0117] When the second bubble sensor 250 detects that no cell liquid starts to flow in the main pipeline 270, and the third bubble sensor 260 still has cell liquid flowing through, at this time, the air has not entered the quantitative tube, or a small amount of air enters the quantitative tube, the quantitative tube is driven to rotate by the quantitative tube rotating member 221, so that the dispensing assembly can empty the cell liquid remaining in the quantitative tube, and further reduce the waste of cell liquid in the main pipeline 270.

[0118] In the embodiment of the present application, when the drop funnel rotating member 231 drives the drop funnel 230 to rotate at a first predetermined speed, the controller simultaneously controls the quantitative tube rotating member 221 to drive the quantitative tube to rotate at a second predetermined speed, so that the cell liquid remaining in the drop funnel 230 will not be left in the quantitative tube again, thereby shortening the time for the air to empty the main pipeline 270, and improving the emptying efficiency of the dispensing control device.

[0119] In the embodiment of the present application, the dispensing assembly further comprises a fourth bubble sensor 320, the fourth bubble sensor 320 is electrically connected with the controller, and the fourth bubble sensor 320 is arranged at one end of the branch pipeline close to the main pipeline 270. In the embodiment, the fourth bubble sensor 320 is clamped at one end of the branch pipeline close to the main pipeline 270, and the fourth bubble sensor 320 is used to detect whether there is cell liquid flowing through the branch pipeline in real time. When the fourth bubble sensor 320 detects that no cell liquid starts to flow in the branch pipeline, it indicates that the cell liquid in the main pipeline has all entered the branch pipeline. By arranging multiple bubble sensors in the pipeline, the bubble condition in the pipeline can be detected, and the dispensing precision of the cell liquid can be improved.

[0120] In the embodiment of the present application, the dispensing assembly further comprises a gas pressure sensor 310, the gas pressure sensor 310 is electrically connected with the controller, and the gas pressure sensor 310 is arranged at one end of the branch pipeline away from the main pipeline 270. The gas pressure sensor 310 is used to monitor the liquid pressure of the branch pipeline in real time when the cell liquid is dispensed into the preparation bag 650. If the pressure detected by the gas pressure sensor 310 is greater than a preset pressure value, the gas pressure sensor 310 sends a feedback signal, and the controller controls the second pump body 210 to reverse, so as to reduce the flow rate of the main pipeline 270, and reduce the liquid pressure in the main pipeline 270 and the branch pipeline. By arranging the gas pressure sensor 310 in the branch pipeline, the pressure in the pipeline can be detected, so as to discharge the excess gas in the preparation bag 650.

[0121] In the embodiment of the present application, the cell liquid dispensing device further comprises a control device, the control device comprising a touch screen component 660 and a start button 670, the touch screen component 660 and the start button 670 are arranged on one side of the body 600, and the touch screen component 660 and the start button 670 are electrically connected with the controller. The touch screen component 660 sets, modifies and monitors the cell liquid dispensing device through the touch screen man-machine interface. The touch screen component 660 is hinged with the body 600, and can be rotated at any angle from 0° to 180° according to the visual angle requirement of the operator.

[0122] The working principle of the cell liquid dispensing device is as follows: press the start button 670, wait for several seconds, and then the device starts to work. The refrigeration component starts to refrigerate. The user can set the relevant parameters through the man-machine interface of the touch screen component 660. Open the first door body 630, install the consumables, open the second door body 640 and the sample bag placing device 500, place the sample bag 140, install the connected pipeline, check whether the consumables are installed correctly, and then close the first door body 630 and the second door body 640. Press the start button on the man-machine interface touch screen to process the sample. The device starts to mix and dispense. After the sample processing is completed, open the front door 6 to heat seal and package the preparation bag 650 and take it out. At this time, the dispensing work is completed.

[0123] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A cell fluid packaging device, characterized in that: include: a body, having a cavity provided therein; The subpackaging device comprises a subpackaging panel and a subpackaging component, wherein the subpackaging panel is arranged in an up-down direction and divides the cavity into a mixing and refrigeration cavity and a subpackaging cavity, and the subpackaging component is arranged on the subpackaging panel; A mixing device, disposed in the mixing refrigeration chamber; A sample bag placement device is provided in the mixing and refrigeration chamber and is connected to the mixing device; A temperature control device is provided in the mixing and refrigeration chamber, and is used to keep the mixing and refrigeration chamber and the filling chamber at a predetermined temperature; The sub-packaging device includes a first sub-packaging panel, a second sub-packaging panel, and a third sub-packaging panel. The first sub-packaging panel is arranged on the upper part of the second sub-packaging panel, and the second sub-packaging panel is arranged on the upper part of the third sub-packaging panel. The lower edges of the first sub-packaging panel and the second sub-packaging panel are both provided with a collecting groove. The bottom of the body is provided with a drainage groove, and the drainage groove is connected to the collecting groove. The subpackaging components include: A flow detection assembly includes a main line, a second pump body located on the third sub-packaging panel, and a flow detection component, wherein the second pump body is arranged on the main line; the flow detection component is connected in series to the main line and is located downstream of the second pump body; A delivery assembly, comprising a pipeline assembly, and a first pump body, a first control valve, and a second control valve located on the third filling panel. The pipeline assembly comprises a first pipeline, a second pipeline, and an air intake pipeline. The first pipeline is respectively connected to the sample bag and the mixing bag, and the first pump body is disposed in the first pipeline; the second pipeline is respectively connected to the first pipeline and the air intake pipeline, the first control valve is disposed in the second pipeline, and the second control valve is disposed in the air intake pipeline. Two sub-packaging assemblies, one of which is disposed on the first sub-packaging panel, and the other of which is disposed on the second sub-packaging panel, the sub-packaging assemblies comprising a plurality of sub-packaging control valves, branch lines connected to the main line, and a plurality of sub-packaging tubes connected to the branch lines, the plurality of sub-packaging control valves being disposed in a one-to-one correspondence with the plurality of sub-packaging tubes, and the plurality of sub-packaging tubes being in one-to-one correspondence with the preparation bags; a controller electrically connected to the first pump body, the first control valve, the second control valve, the sub-packaging control valve, the second pump body, and the flow detection element respectively; The flow detection component also includes: a drip bucket, connected in series to the main pipe and located between the second pump body and the first control valve; a drip bucket rotating member, disposed on the third filling panel, connected to the drip bucket, electrically connected to the controller, and used to drive the drip bucket to rotate; The flow detection component is a quantitative tube, and the flow detection component further includes: The quantitative tube rotating member is provided on the second filling panel, is connected to the quantitative tube, is electrically connected to the controller, and is used to drive the quantitative tube to rotate.

2. The cell fluid packaging equipment according to claim 1, characterized in that: The front end of the body is provided with a first opening communicating with the filling chamber, the first opening is provided with a first door connected to the body, the first side wall of the body is provided with a second opening communicating with the mixing and refrigeration chamber, and the second opening is provided with a second door connected to the body.

3. The cell fluid packaging equipment according to claim 2, characterized in that: The sample bag placement device comprises: A fixed seat connected to the mixing device; a telescopic component, a first end of which is connected to the fixing seat, and the telescopic component is adapted to switch between an extended state and a shortened state; A sample placement box is connected to the second end of the telescopic component and is used to place a sample bag. In the extended state, the sample placement box extends through the second opening. In the shortened state, the sample placement box is located in the mixing and refrigeration chamber.

4. The cell fluid packaging equipment according to claim 3, characterized in that: The sample bag placement device also includes: A carrying plate is arranged above the sample placement box in a downwardly inclined manner, and the sample bag is placed on the carrying plate; The sample bag tensioning component is arranged below the sample placement box and connected to the body. The sample bag tensioning component is connected to the liquid outlet pipe of the sample bag. The sample bag tensioning component is used to tension the liquid outlet pipe of the sample bag downward so that the liquid outlet pipe of the sample bag is located below the sample bag.

5. The cell fluid packaging device according to any one of claims 2 to 4, characterized in that: The mixing device comprises: Base components; a cold plate disposed on an upper portion of the base component, the cold plate being disposed opposite to the second opening; a mixing plate component, movably disposed on a side of the cold plate facing the second opening; The driving component is arranged on the side of the cold plate away from the second opening and is connected to the mixing plate component; the driving component is used to drive the mixing plate component to squeeze the mixing bag so that the liquid in the mixing bag is evenly mixed.

6. The cell fluid packaging equipment according to claim 5, characterized in that: The mixing plate component includes: a mixing plate, wherein a first end of the mixing plate is hinged to the driving component; a fixing member connected to the driving member; A locking piece is provided at the second end of the mixing plate, and the locking piece is locked and matched with the fixing piece.

7. The cell fluid packaging equipment according to claim 5, characterized in that: The temperature control device comprises: A refrigeration component, comprising an evaporator, a condenser, a compressor, a capillary tube, and a first fan, wherein the evaporator is disposed on a side of the cold plate facing away from the second opening, the evaporator is in fluid communication with the condenser, the compressor, and the capillary tube, and the condenser and the compressor are disposed on the base component; an air outlet is disposed on the second side wall of the body, and the first fan is disposed on one side of the condenser, the first fan being arranged corresponding to the air outlet; The temperature sensor is arranged on a side of the cold plate away from the second opening.

8. The cell fluid packaging equipment according to claim 7, characterized in that: The temperature control device also includes: The air supply component includes an air duct, at least one second fan and a heat sink. The air duct is arranged on the side of the cold plate away from the second opening. The air duct has an air inlet and an air outlet. The heat sink is arranged inside the air duct and is attached to the cold plate. At least one of the air inlet and the air outlet of the air duct is provided with the second fan, and the air inlet and the air outlet of the air duct are both connected to the sub-packaging cavity through a pipeline.

Images