A whitening and freckle-removing composition for sensitive skin, and a preparation method and application thereof

Abstract

The present application relates to a kind of whitening and freckle-removing composition for sensitive skin and its preparation method and application, belong to the technical field of cosmetics.The whitening and freckle-removing composition for sensitive skin of the present application includes the following components: oxidized white resveratrol microspheres, acetyl hexapeptide-1, leaf extract of mountain maytree, butterfly orchid extract, hibiscus extract;The mass ratio of the oxidized white resveratrol microspheres, acetyl hexapeptide-1, leaf extract of mountain maytree, butterfly orchid extract, hibiscus extract is (1-5):(0.01-0.2):(0.1-1):(1-3):(0.5-1.5).The whitening and freckle-removing composition of the present application adopts the oxidized white resveratrol microspheres with strong light stability and acetyl hexapeptide-1, leaf extract of mountain maytree, butterfly orchid extract, hibiscus extract reasonable compounding, give full play to the synergistic effect between components, can play a role from the generation, transport, decomposition of melanin and skin water, moisturizing and other multiple links, with whitening, freckle-removing, moisturizing and barrier repair effect.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a whitening and freckle-removing composition for sensitive skin and a preparation method and application thereof. BACKGROUND

[0002] The color of skin is determined by the content and distribution of skin pigments, among which melanin is the main decisive factor. Melanin is a pigment polymer synthesized in melanosome by melanocytes, then transferred to keratinocytes through the dendritic processes of melanocytes, and up to the stratum corneum with the epidermal cells, and finally excreted with the shedding of the stratum corneum. Accumulation of melanin will form skin problems such as uneven skin color and freckles, affecting people's quality of life. In addition, dry skin can also cause barrier function damage, leading to dry skin, itching, easy allergy, easy wrinkles, freckles and other problems.

[0003] Current whitening products focus on considering the generation of melanin, and there are few products that comprehensively consider multiple links such as the generation and transport of melanin. The long-term whitening effect is not good, and the current whitening and freckle-removing moisturizing products have greater irritation, poor barrier repair function, and are difficult to apply to sensitive skin.

[0004] Therefore, it is of great significance to develop a whitening and freckle-removing composition for sensitive skin that can effectively whiten and moisturize, and at the same time effectively repair the skin barrier. SUMMARY

[0005] The present application aims to overcome the problems existing in the prior art, and provides a whitening and freckle-removing composition for sensitive skin and a preparation method and application thereof.

[0006] The present application is realized by the following technical scheme:

[0007] In a first aspect, the present application provides a whitening and freckle-removing composition for sensitive skin, comprising the following components: oxidized resveratrol microspheres, acetyl hexapeptide-1, leaf extract of mountain rosebay, butterfly orchid extract, and hibiscus extract; the mass ratio of the oxidized resveratrol microspheres, acetyl hexapeptide-1, leaf extract of mountain rosebay, butterfly orchid extract, and hibiscus extract is (1-5):(0.01-0.2):(0.1-1):(1-3):(0.5-1.5).

[0008] The whitening and freckle-removing composition of this invention uses a rational blend of oxidized resveratrol microspheres, acetyl hexapeptide-1, loquat leaf extract, Phalaenopsis orchid extract, and hibiscus extract. It provides long-lasting whitening and moisturizing effects, effectively eliminates free radicals, and repairs the skin barrier, making it suitable for sensitive skin. The oxidized resveratrol of this invention has a strong inhibitory effect on tyrosinase, effectively inhibiting melanin synthesis and transport. It also eliminates free radicals, exerting a powerful antioxidant effect, and has certain firming, soothing, and skin barrier repair functions. Furthermore, this invention selects oxidized resveratrol microspheres to encapsulate the oxidized resveratrol within the microspheres, achieving a sustained-release effect. After application, it can inhibit melanin production and transport for a long time.

[0009] The acetyl hexapeptide-1 of this invention can inhibit the activity of tyrosinase and the production of melanin, and reduce the number of melanin dendrites, thus achieving a skin whitening and brightening effect. The active ingredients in loquat leaf extract can activate the autophagy mechanism of melanosomes and improve pigmentation. This invention selects oxidized resveratrol microspheres, acetyl hexapeptide-1, and loquat leaf extract in combination, which can give full play to the synergistic effect between the components and comprehensively enhance the whitening effect from three aspects: inhibiting the synthesis and transport of melanin and promoting the decomposition of melanin.

[0010] In addition, Phalaenopsis orchid extract can effectively increase the ability of keratinocytes to secrete hyaluronic acid, and can also repair skin damage caused by radiation at night, and increase skin elasticity. Hibiscus extract can soothe and relieve allergies, giving the skin a cooling sensation, while also having antibacterial, anti-inflammatory, and anti-swelling effects, as well as good moisturizing effects. The oxidized resveratrol microspheres of this invention, combined with Phalaenopsis orchid extract and Hibiscus extract, can effectively moisturize and improve the skin barrier function. Furthermore, the rational combination of oxidized resveratrol microspheres, acetyl hexapeptide-1, loquat leaf extract, Phalaenopsis orchid extract, and Hibiscus extract can fully exert the synergistic effect between components, resulting in an excellent overall effect of the whitening and spot-removing composition.

[0011] Preferably, the mass ratio of the oxidized resveratrol microspheres, acetyl hexapeptide-1, loquat leaf extract, Phalaenopsis orchid extract, and hibiscus extract is (1-3):(0.01-0.1):(0.1-0.5):(1-2):1.

[0012] Furthermore, the mass ratio of the oxidized resveratrol microspheres to the combined mass of the Phalaenopsis orchid extract and the Hibiscus rosa-sinensis extract is (1-1.5):1; even further, the mass ratio of the oxidized resveratrol microspheres, acetyl hexapeptide-1, loquat leaf extract, Phalaenopsis orchid extract, and Hibiscus rosa-sinensis extract is 3:0.03:0.5:1:1.

[0013] Preferably, the hibiscus extract includes at least one of hibiscus flower extract and hibiscus leaf extract.

[0014] Preferably, the hibiscus extract includes hibiscus flower extract and hibiscus leaf extract, wherein the mass ratio of the hibiscus flower extract to the hibiscus leaf extract is (1-2):1.

[0015] Preferably, the oxidized resveratrol microspheres include a shell and a capsule encapsulated inside the shell;

[0016] The outer shell comprises calcium ion crosslinked sodium alginate hydrogel, graphene oxide, and cellulose;

[0017] The contents include oxidized resveratrol inclusions and water. The oxidized resveratrol inclusions include oxidized resveratrol and a long-chain macromolecular alcohol encapsulated around the oxidized resveratrol. The long-chain macromolecular alcohol includes at least one of 1,8-octanediol and 1,2-octanediol.

[0018] The mass ratio of the oxidized resveratrol inclusion, the calcium ion crosslinked sodium alginate hydrogel, the oxidized graphene, and the water is (2.5–10) g:(0.5–60) mg:(0.15–300) mg:1 g;

[0019] The mass ratio of the oxidized resveratrol inclusion to the long-chain macromolecular alcohol is (4-7):(4-7);

[0020] The mass ratio of the calcium ion-crosslinked sodium alginate hydrogel to the cellulose is (0.1-1):(0.1-0.8).

[0021] The outer shell membrane material of the oxidized resveratrol microspheres of the present invention can reduce the transmittance of UVA and UVB, and increase the absorbance and reflectance of UVA and UVB, thereby effectively improving the photostability of oxidized resveratrol and reducing the stimulating effect of ultraviolet rays on melanocytes and melanin dendrites. In addition, the long-chain macromolecular octanediol in the oxidized resveratrol microspheres has a water-binding effect, and when combined with Phalaenopsis orchid extract and Hibiscus rosa-sinensis leaf extract, it can enhance the moisturizing effect and improve the skin barrier function.

[0022] Further, the mass ratio of the oxidized resveratrol inclusion, the calcium ion crosslinked sodium alginate hydrogel, the graphene oxide, and the water is (2.5–10) g:(0.5–30) mg:(1–150) mg:1 g; even further, the mass ratio of the oxidized resveratrol inclusion, the calcium ion crosslinked sodium alginate hydrogel, the graphene oxide, and the water is (2.5–10) g:(0.5–10) mg:(0.15–50) mg:1 g.

[0023] Preferably, the method for preparing the oxidized resveratrol microspheres includes the following steps:

[0024] (1) Dissolve oxidized resveratrol and long-chain macromolecular alcohol in a good solvent to prepare a first solution; add an antisolvent to the first solution to cause the long-chain macromolecular alcohol to encapsulate the oxidized resveratrol and precipitate out, thereby obtaining oxidized resveratrol inclusions;

[0025] (2) The encapsulated body obtained in step (1) is dispersed in water as the inner phase liquid, and sodium alginate, graphene oxide and cellulose are dispersed in water as the outer phase liquid. Microdroplets are prepared by coaxial flow gas jet technology. The outside of the microdroplet is the outer phase liquid and the inside of the microdroplet is the inner phase liquid. The outside of the microdroplet encapsulates the inside of the microdroplet. The microdroplet is then dropped into a receiving liquid containing calcium chloride to obtain the oxidized resveratrol microspheres.

[0026] In the preparation method of oxidized resveratrol microspheres of the present invention, the principle of coaxial flow gas jet technology is as follows: a coaxial device is provided, including an inner phase shaft tube, an outer phase shaft tube, and a drive shaft tube. The outer phase shaft tube is nested inside the drive shaft tube, and the inner phase shaft tube is nested inside the outer phase shaft tube. The inner phase shaft tube, the outer phase shaft tube, and the drive shaft tube are all provided with inlets and outlets communicating with the outside. The inlets of the inner phase shaft tube, the outer phase shaft tube, and the drive shaft tube are independent of each other. The outlets of the inner phase shaft tube, the outer phase shaft tube, and the drive shaft tube are located at the same position, and the dimensions of the outlets of the inner phase shaft tube, the outer phase shaft tube, and the drive shaft tube are all reduced to a tiny size. The inner phase liquid is introduced through the inlet of the inner phase shaft tube, the outer phase liquid through the inlet of the outer phase shaft tube, and the gas through the inlet of the drive shaft tube. The inner phase liquid, outer phase liquid, and gas flow to the outlets of the inner phase shaft tube, outer phase shaft tube, and drive shaft tube, respectively, and are ejected and converge at their respective outlets. Under the shearing action of the gas, microdroplets with an outer phase liquid on the outside and an inner phase liquid on the inside are formed. Furthermore, a receiving liquid containing calcium chloride can be placed below the microdroplets. After the microdroplets are dropped into the receiving liquid, the calcium ions in the calcium chloride rapidly crosslink with sodium alginate to form a calcium ion crosslinked sodium alginate hydrogel. Cellulose and graphene oxide are doped into the hydrogel, which enhances the strength of the microsphere shell and increases the structural stability of the microspheres.

[0027] Preferably, in step (2), the flow rate of the inner phase liquid is 0.01 mL / h to 6 mL / h, and the flow rate of the outer phase liquid is 0.05 mL / h to 600 mL / h; further, the gas used for driving in the coaxial flow gas jet may include, for example, air, nitrogen, compressed gas, etc., with a gas pressure of 0.5 MPa to 2 MPa and a flow rate of 5 NL / min to 20 NL / min.

[0028] The resveratrol oxidized inclusion complex of this invention utilizes a coaxial microfluidic device and employs the antisolvent principle to precipitate resveratrol oxidized by encapsulating long-chain macromolecular alcohols. The antisolvent refers to a poor solvent for the solute; adding a large amount of antisolvent to a solvent system can cause a specific solute to precipitate due to decreased solubility. In the preparation method of this invention, the long-chain macromolecular alcohol and resveratrol oxidized are first dissolved in a good solvent to ensure complete dissolution and mixing. Then, the antisolvent is added. The antisolvent is a poor solvent for the long-chain macromolecular alcohol and resveratrol oxidized and cannot dissolve them. The first solution diffuses in the antisolvent, and the long-chain macromolecular alcohol and resveratrol oxidized precipitate due to decreased solubility. Furthermore, since long-chain macromolecular alcohols have relatively long flexible chains and oxidized resveratrol has relatively short rigid chains, under appropriate content ratios, long-chain macromolecular alcohols can encapsulate oxidized resveratrol molecules to prevent oxidized resveratrol from directly contacting oxygen, thereby effectively protecting the activity of oxidized resveratrol.

[0029] Preferably, step (1) is performed using a coaxial microfluidic device, with the first solution as the inner phase and the antisolvent as the outer phase. Further, the flow rate of the inner phase can be, for example, but not limited to, 0.01 mL / h to 6 mL / h, and the flow rate of the outer phase can be, for example, but not limited to, 0.05 mL / h to 600 mL / h.

[0030] Preferably, the good solvent includes short-chain small molecule alcohols, which are alcohol compounds with 2 to 4 carbon atoms. Further, the short-chain small molecule alcohol includes at least one selected from 1,3-butanediol, 1,3-propanediol, and ethanol. The above-mentioned short-chain small molecule alcohols exhibit good solubility for both long-chain large molecule alcohols and oxidized resveratrol, and have low raw material costs.

[0031] Preferably, the antisolvent includes water. Water cannot dissolve long-chain macromolecular alcohols or oxidized resveratrol. Moreover, water is a low-cost and safe raw material. Using water as an antisolvent allows the oxidized resveratrol inclusions formed by encapsulating oxidized resveratrol in long-chain macromolecular alcohols to be granular and precipitated efficiently. Furthermore, it has little impact on the activity of the obtained oxidized resveratrol inclusions themselves, resulting in a safe and friendly product.

[0032] Preferably, step (1) is performed under light-protected conditions.

[0033] Preferably, in step (2), the mass ratio of sodium alginate to water in the external phase liquid is (0.5-2) mg:1g, and the mass ratio of graphene oxide to water is (0.3-5) mg:1g; the concentration of calcium chloride in the receiving liquid containing calcium chloride is (2-15) mg / mL.

[0034] Preferably, the method for preparing the loquat leaf extract includes the following steps: sieving dried and pulverized loquat leaves, mixing them with a propylene glycol solution, ultrasonically extracting, centrifuging, and filtering to obtain the loquat leaf extract.

[0035] Preferably, in the method for preparing the loquat leaf extract, the sieving is performed through a 30-40 mesh sieve, the concentration of the propylene glycol solution is 45wt%-55wt%, the mass ratio of the propylene glycol solution to the sieved loquat leaves is (15-25):1, and the ultrasonic extraction time is 80min-100min.

[0036] Preferably, the preparation method of the Phalaenopsis orchid extract includes the following steps: sieving dried and pulverized Phalaenopsis orchid flowers, mixing with water, ultrasonically extracting, centrifuging, and filtering to obtain the Phalaenopsis orchid extract.

[0037] Preferably, in the preparation method of the Phalaenopsis extract, the sieving is performed through a 30-40 mesh sieve, the mass ratio of water to the sieved Phalaenopsis is (15-25):1, and the ultrasonic extraction time is 90-110 min.

[0038] Preferably, the preparation method of the hibiscus extract includes the following steps: sieving dried and pulverized hibiscus (leaves or flowers), mixing with ethanol solution, ultrasonic extraction, filtration, and concentration to obtain the hibiscus leaf extract.

[0039] Preferably, in the preparation method of the hibiscus extract, the sieving is performed through a 30-40 mesh sieve, the concentration of the ethanol solution is 45wt%-55wt%, the mass ratio of the ethanol solution to the sieved hibiscus is (5-10):1, and the ultrasonic extraction time is 50min-70min.

[0040] Furthermore, the preparation method of the hibiscus leaf extract includes the following steps: sieving the dried and pulverized hibiscus leaves, mixing them with an ethanol solution, ultrasonically extracting, filtering, and concentrating to obtain the hibiscus leaf extract.

[0041] The preparation method of the hibiscus flower extract includes the following steps: sieving dried and pulverized hibiscus flowers, mixing them with an ethanol solution, ultrasonically extracting, filtering, and concentrating to obtain the hibiscus flower extract.

[0042] Secondly, the present invention provides a method for preparing the whitening and freckle-removing composition for sensitive skin, comprising the following steps: mixing oxidized resveratrol microspheres, acetyl hexapeptide-1, loquat leaf extract, Phalaenopsis orchid extract and hibiscus extract evenly according to the mass ratio, thereby obtaining the whitening and freckle-removing composition.

[0043] Thirdly, the present invention provides the application of the whitening and spot-removing composition for sensitive skin in cosmetics.

[0044] The whitening and freckle-removing composition of the present invention has excellent whitening, freckle-removing, and moisturizing effects, is suitable for sensitive skin, and also has a certain effect on repairing the skin barrier. It can be widely used in cosmetics to form different formulations, including but not limited to skin care products such as toners, lotions, creams, masks, serums, sprays, sunscreens, sunscreen sprays, and sunscreen lotions, as well as various makeup products.

[0045] Fourthly, the present invention provides a skin care product comprising the whitening and spot-removing composition for sensitive skin, wherein the whitening and spot-removing composition for sensitive skin is present in the skin care product at a mass percentage of 3%-10%.

[0046] Preferably, the skin care product comprises the following components by weight percentage: 3%-10% of the whitening and spot-removing composition for sensitive skin, 2%-20% of the emulsifier, 1%-6% of the emulsifier, 0.1%-2% of the thickener, 0.3%-2% of the preservative, 0.1%-0.5% of the stabilizer, 0.05%-1% of the antioxidant, and the balance being water.

[0047] Furthermore, the skincare product comprises the following components by weight percentage: 3%-10% of the whitening and spot-removing composition for sensitive skin, 3%-15% of the emulsifier, 1%-3% of the emulsifier, 0.1%-0.5% of the thickener, 0.5%-1% of the preservative, 0.1%-0.3% of the stabilizer, 0.2%-1% of the antioxidant, and the balance being water.

[0048] This invention does not impose any particular restrictions on the types of components other than the skin whitening and freckle-removing composition in skin care products. For example, the emollient may include at least one of caprylic / capric triglyceride, dioctyl carbonate, and propylene glycol carbonate; the emulsifier may include at least one of glyceryl stearate, PEG-100 stearate, sorbitan stearate, C14-22 alcohol, and C12-20 alkyl glucoside; the thickener may include at least one of xanthan gum, carbomer, and sodium polyacrylate; the preservative may include at least one of p-hydroxyacetophenone, hexanediol, phenoxyethanol, and ethylhexylglycerin; the stabilizer may include arginine and aminomethylpropanol; and the antioxidant may include at least one of ascorbic acid tetraisopalmitate, tocopheryl acetate, and sodium metabisulfite.

[0049] This invention offers the following beneficial effects: The whitening and spot-removing composition for sensitive skin utilizes photostable resveratrol microspheres and a rational combination of acetyl hexapeptide-1, loquat leaf extract, Phalaenopsis orchid extract, and Hibiscus rosa-sinensis extract. This combination fully leverages the synergistic effects between the components, working on multiple stages including melanin production, transport, decomposition, and skin hydration to achieve whitening, moisturizing, and spot removal. Furthermore, this whitening and spot-removing composition not only possesses excellent whitening, moisturizing, and spot-removing effects but also has a certain effect on repairing the skin barrier function, and is friendly to sensitive skin. Detailed Implementation

[0050] To better illustrate the objectives, technical solutions, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0051] Unless otherwise specified, the experimental methods used in the examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified.

[0052] Acetyl hexapeptide-1 was purchased from Zhejiang Paipai Biotechnology Co., Ltd.; the oxidized resveratrol inclusion complex in Comparative Example 8 was purchased from Naturalis, brand name NIO-OXY; the Rhodiola rosea extract, aloe vera extract, and seaweed extract were all commercially available.

[0053] The whitening and freckle-removing compositions of the examples and comparative examples are made from the components in parts by mass listed in Table 1.

[0054] Table 1. Component mass parts (parts)

[0055]

[0056] The preparation method of the whitening and freckle-removing compositions of Examples 1-8 and Comparative Examples 1-7 is as follows: the components are mixed according to the mass parts described in Table 1 to obtain the whitening and freckle-removing compositions.

[0057] The preparation method of oxidized resveratrol microspheres includes the following steps:

[0058] (1) Dissolve 70 mg of oxidized resveratrol and 40 mg of 1,8-octanediol in 1 mL of 1,3-butanediol under light-protected conditions to prepare the first solution; use a coaxial microfluidic device with a capillary glass tube diameter of 0.1 mm for the inner phase and 1 mm for the outer phase. Flow the first solution into the inner phase and pure water into the outer phase. Use the anti-solvent method to precipitate oxidized resveratrol encapsulated in 1,8-octanediol. Collect the effluent at the tube opening in a brown bottle. After lyophilizing the effluent to evaporate the solvent, oxidized resveratrol inclusions are obtained; adjust the flow rate of the inner phase to 6 mL / h and the flow rate of the outer phase to 240 mL / h.

[0059] (2) Disperse 5g of the inclusion body obtained in step (1) in 1g of water as the inner phase liquid, and then disperse 1mg of sodium alginate, 3mg of powdered graphene oxide and 0.5mg of cellulose in 1g of water as the outer phase liquid. Using coaxial flow gas jet technology, the first inner phase liquid and the first outer phase liquid are ejected from the shaft tube under the driving action of gas to form microdroplets. The outside of the microdroplet is the outer phase liquid and the inside of the microdroplet is the inner phase liquid. The outside of the microdroplet encapsulates the inside of the microdroplet. The flow rate of the inner phase liquid is 3mL / h and the flow rate of the outer phase liquid is 6mL / h. The gas is nitrogen, the gas pressure is 1MPa and the flow rate is 15NL / min.

[0060] The microdroplets are then dropped into a receiving solution with a calcium chloride concentration of 5 mg / mL. The calcium ions crosslink with sodium alginate to form a hydrogel, thereby forming a microsphere shell on the outside of the microdroplets, thus obtaining the oxidized resveratrol microspheres.

[0061] The preparation method of loquat leaf extract is as follows: Purchase commercially available loquat leaves, dry them, pulverize them, pass them through a 40-mesh sieve, mix them with a 50wt% propylene glycol solution at a mass ratio of 1:20, extract them using ultrasound for 90 minutes, centrifuge and filter them to obtain loquat leaf extract.

[0062] The preparation method of Phalaenopsis orchid extract is as follows: Purchase commercially available Phalaenopsis orchids, dry them, pulverize them, pass them through a 40-mesh sieve, mix them with distilled water at a mass ratio of 1:20, extract them using ultrasound for 2 hours, centrifuge and filter them to obtain Phalaenopsis orchid extract.

[0063] The preparation method of Hibiscus leaf extract is as follows: Purchase commercially available Hibiscus leaves, dry them, pulverize them, pass them through a 40-mesh sieve, mix them with 50wt% ethanol aqueous solution at a mass ratio of 1:8, extract with ultrasonic stirring for 1 hour, filter and collect the filtrate, concentrate under reduced pressure to remove ethanol, and obtain Hibiscus leaf extract.

[0064] The preparation method of hibiscus flower extract is as follows: purchase commercially available hibiscus flowers, dry them, pulverize them, pass them through a 40-mesh sieve, mix them with 50wt% ethanol aqueous solution at a mass ratio of 1:8, extract them by ultrasonic stirring for 1 hour, filter and collect the filtrate, concentrate it under reduced pressure to remove ethanol, and obtain hibiscus flower extract.

[0065] Comparative Example 8

[0066] The difference between this comparative example and Example 1 is that commercially available resveratrol oxidized inclusions are used instead of resveratrol oxidized microspheres, so that the content of resveratrol oxidized in the commercially available resveratrol oxidized inclusions is equal to the content of resveratrol oxidized in the resveratrol oxidized microspheres. The other components and contents are the same as in Example 1. In the preparation method, only the resveratrol oxidized microspheres are replaced with the commercially available resveratrol oxidized inclusions of this comparative example. Everything else is the same as in Example 1, resulting in the whitening and freckle-removing composition of this comparative example.

[0067] Comparative Example 9

[0068] The difference between this comparative example and Example 1 is that resveratrol oxide powder is used instead of resveratrol oxide microspheres, and the amount of resveratrol oxide powder is equal to the content of resveratrol oxide in the resveratrol oxide microspheres. The remaining components and contents are the same as in Example 1. In the preparation method, the resveratrol oxide microspheres are replaced with the resveratrol oxide powder of this comparative example, and the resveratrol oxide powder is dissolved in butylene glycol to the same concentration as the resveratrol oxide in the resveratrol oxide microspheres. The rest is the same as in Example 1, thus obtaining the whitening and freckle-removing composition of this comparative example.

[0069] Comparative Example 10

[0070] The difference between this comparative example and Example 1 is that an equal amount of Rhodiola rosea extract is used to replace loquat leaf extract, while the remaining components and contents are the same as in Example 1; in the preparation method, only the loquat leaf extract is replaced with Rhodiola rosea extract of this comparative example, while the rest are the same as in Example 1, to obtain the whitening and freckle-removing composition of this comparative example.

[0071] Comparative Example 11

[0072] The difference between this comparative example and Example 1 is that an equal amount of aloe vera extract is used to replace the orchid extract, while the remaining components and contents are the same as in Example 1; in the preparation method, only the orchid extract is replaced with the aloe vera extract of this comparative example, while the rest are the same as in Example 1, to obtain the whitening and freckle-removing composition of this comparative example.

[0073] Comparative Example 12

[0074] The difference between this comparative example and Example 1 is that an equal amount of seaweed extract is used instead of hibiscus extract, while the remaining components and contents are the same as in Example 1; in the preparation method, only the hibiscus extract is replaced with the seaweed extract of this comparative example, while the rest are the same as in Example 1, to obtain the whitening and freckle-removing composition of this comparative example.

[0075] The whitening and freckle-removing compositions of each embodiment and comparative example were prepared into skin care products, which were made according to the weight percentage components in Table 2:

[0076] Table 2. Mass percentage of each component in skincare products

[0077]

[0078] The preparation method of the skin care product is as follows: Moisturizer, emulsifier, and antioxidant are mixed and heated to 80℃ to obtain the oil phase; thickener, stabilizer, and water are mixed and heated to 80℃ to obtain the aqueous phase; the oil phase is added to the aqueous phase and homogenized to obtain an emulsion; after cooling, a whitening and spot-removing composition and preservative are added to obtain the skin care product. Example 1: Experiment on the inhibitory ability of tyrosinase.

[0079] The whitening and freckle-removing compositions of the examples and comparative examples were exposed to light for 1 hour, and the inhibitory ability of the compositions on tyrosinase after light exposure was tested. The test results are shown in Table 3.

[0080] Test method: Positive control: diluted with PBS buffer to a series of concentration gradients of 1 mg / mL, 0.2 mg / mL, 0.04 mg / mL, and 0.008 mg / mL to verify the test system;

[0081] Test sample preparation: Take equal amounts of the whitening and freckle-removing compositions of the examples and comparative examples that have been exposed to light for 1 hour as test samples, and dilute each group of test samples with PBS buffer to prepare 2wt% sample test solution;

[0082] Prepare test tubes A, B, C, and D for each test sample according to the following equipment requirements. Prepare blank control tubes. After preparation, add 0.5 mL of L-DOPA solution (with L-DOPA mass percentage of 5%) to each tube. Incubate the test tubes and blank control tubes at 37℃ for 30 min. Measure their absorbance at 475 nm. Repeat the experiment 3 times for each sample.

[0083] The solution preparation requirements for test tubes A, B, C, and D are as follows: Tube A: 3 mL PBS buffer and 0.5 mL tyrosinase; Tube B: 3.5 mL PBS buffer; Tube C: 1 mL sample solution, 2 mL PBS buffer, and 0.5 mL tyrosinase; Tube D: 1 mL sample solution and 2.5 mL PBS buffer.

[0084] Zeroing: PBS solution;

[0085] The tyrosinase inhibition rate of the test sample was calculated using the following formula:

[0086] Tyrosinase inhibition rate = 1 - (CD) / (AB) × 100%; where A is the absorbance value of tube A, B is the absorbance value of tube B, C is the absorbance value of tube C, and D is the absorbance value of tube D.

[0087] Table 3. Results of tyrosinase inhibition tests on the compositions of the examples and comparative examples.

[0088] Group Tyrosinase inhibition rate / % Example 1 91.08％ Example 2 75.93％ Example 3 82.76％ Example 4 65.91％ Example 5 66.61％ Example 6 77.19％ Example 7 74.99％ Example 8 83.82％ Comparative Example 1 23.91％ Comparative Example 2 31.44％ Comparative Example 3 49.64％ Comparative Example 4 52.55％ Comparative Example 5 53.12％ Comparative Example 6 39.55％ Comparative Example 7 32.28％ Comparative Example 8 53.26％ Comparative Example 9 35.57％ Comparative Example 10 47.21％ Comparative Example 11 41.91％ Comparative Example 12 42.99％

[0089] As can be seen from Table 3, the compositions of Examples 1-8 still have a significant ability to inhibit tyrosinase after 1 hour of light exposure, indicating that the whitening effect of the compositions of the present invention is excellent.

[0090] Comparing Example 1 and Comparative Examples 1-5, it can be seen that the absence of any component in the composition affects the inhibitory activity of tyrosinase, leading to a decrease in the whitening effect of the composition. In Comparative Examples 6 and 7, the poor ratio between components resulted in a decreased synergistic effect, which also affected the whitening effect of the composition. In Comparative Example 8, commercially available oxidized resveratrol encapsulation was used, which has inferior photostability compared to the oxidized resveratrol microspheres of this invention, resulting in a lower tyrosinase inhibitory activity in the composition compared to Example 1. Comparative Example 9 used unencapsulated oxidized resveratrol powder, which has poor photostability, resulting in a poor inhibitory effect of the composition on tyrosinase activity. Comparative Example 10 used Rhodiola rosea extract instead of loquat leaf extract, Comparative Example 11 used aloe vera extract instead of Phalaenopsis orchid extract, and Comparative Example 12 used seaweed extract instead of Hibiscus rosa-sinensis extract. The synergistic effect with the other components was poor, and the synergistic effect between the components could not be fully utilized, resulting in a decrease in the inhibitory activity of the composition on tyrosinase.

[0091] Example 2: Experiment on scavenging DPPH free radicals

[0092] Test method: (1) Dissolve 0.001972g of DPPH in anhydrous ethanol solution, and make up to 25mL with anhydrous ethanol solution to obtain DPPH test solution;

[0093] (2) Take equal amounts of the whitening and freckle-removing compositions of the examples and comparative examples as test samples. Dilute each group of test samples with PBS buffer to prepare 2wt% sample test solution. Prepare solutions according to the following A, B and C tube solutions respectively. Place them in the dark at room temperature for 30 min. Measure the absorbance at 517nm and record the absorbance value. Repeat each sample 3 times.

[0094] Tube A: 2 mL sample test solution + 2 mL DPPH test solution;

[0095] Tube B: 2 mL DPPH test solution + 2 mL PBS;

[0096] Tube C: 2 mL PBS + 2 mL sample test solution;

[0097] Zeroing: Anhydrous ethanol solution;

[0098] The calculation method is as follows: DPPH free radical scavenging rate = 1 - (AC) / B × 100%, and the experiment is repeated 3 times and the average value is taken; where A is the absorbance value of tube A, B is the absorbance value of tube B, and C is the absorbance value of tube C; the test results are shown in Table 4.

[0099] Table 4. Free radical scavenging test results of the compositions in the examples and comparative examples.

[0100]

[0101]

[0102] As can be seen from Table 4, the compositions of Examples 1-8 have a higher ability to scavenge free radicals than the compositions of Comparative Examples 1-2, indicating that the present invention uses oxidized resveratrol microspheres combined with a variety of plant extracts to make the composition have excellent antioxidant capacity.

[0103] In Comparative Examples 1-5, the absence of any component in the composition led to a decrease in the antioxidant capacity of the composition. Among them, the composition of Comparative Example 1, which lacked oxidized resveratrol microspheres, had the worst antioxidant capacity. In Comparative Examples 6 and 7, the poor ratio between components and the reduced synergistic effect also affected the antioxidant capacity of the composition. Comparative Example 8 used commercially available oxidized resveratrol encapsulation, and Comparative Example 9 used unencapsulated oxidized resveratrol powder. The encapsulation of oxidized resveratrol was inferior to that of the oxidized resveratrol microspheres of the present invention, resulting in a lower free radical scavenging ability of the composition compared to Example 1. Comparative Example 10 used Rhodiola rosea extract instead of loquat leaf extract, Comparative Example 11 used aloe vera extract instead of Phalaenopsis orchid extract, and Comparative Example 12 used seaweed extract instead of Hibiscus rosa-sinensis extract. The synergistic effect with the other components was poor, and the synergistic effect between components could not be fully utilized, resulting in a decrease in the antioxidant capacity of the composition.

[0104] Example 3: Whitening, spot removal, moisturizing, and skin barrier repair efficacy test

[0105] Test Method: Two hundred Asian subjects aged 18-45 years with dry facial skin (skin moisture content <45 C.U.) and sensitive skin (positive lactic acid sting test) were selected for the test. After cleansing their faces with facial cleanser, the subjects patted their faces dry with lint-free absorbent paper towels and then sat quietly for at least 30 minutes until their skin condition stabilized. A skin moisture content tester was then used on each subject. CM 825, Transdermal Moisture Loss Probe TM Hex and the Colorimeter CL400 color probe were used to measure initial values ​​of skin moisture content, TEWL, and ITA°. Subjects were randomly divided into 20 groups of 10 each. All subjects randomly applied the skincare products obtained in the examples and comparisons to their entire face. Testing was conducted at 7 and 14 days, and the improvement observed in each volunteer at these times was analyzed. Improvement was expressed as the rate of improvement relative to the initial value, calculated as follows: Improvement rate relative to initial value = |(X... 使用后 -X 使用前 ) / X 使用前 ×100% | If the difference between the data after sample use and the data before use is significantly increased (P<0.05), then the product is considered to have the corresponding efficacy.

[0106] The test results are shown in Table 5.

[0107] Table 5. Test results of the skin care products in the examples and comparative examples for whitening, spot removal, moisturizing, and skin barrier repair.

[0108]

[0109]

[0110] As can be seen from Table 5, the skin care products of the present invention are suitable for people with sensitive skin and have significant whitening, spot-removing and moisturizing effects. They also have a certain skin barrier repair effect. The skin care products made using the whitening and spot-removing compositions of Examples 1-8 of the present invention have more significant whitening, spot-removing, moisturizing and barrier repair effects than those of Comparative Examples 1-12.

[0111] In Comparative Example 1, the lack of oxidized resveratrol microspheres, in Comparative Example 2, the lack of acetyl hexapeptide-1, and in Comparative Example 3, the lack of loquat leaf extract, resulted in a significant decrease in the improvement of skin ITA° after 14 days of use compared to Example 1. This also reduced the moisturizing and barrier repair effects of the skincare products. In Comparative Example 4, the lack of hibiscus extract, and in Comparative Example 5, the lack of Phalaenopsis orchid extract, resulted in ineffective moisturizing. After 14 days of use, the skin's moisture content was significantly lower than in Example 1, also leading to a decrease in the whitening and barrier repair effects of the skincare products. In Comparative Examples 6 and 7, the composition ratio was poor, resulting in a decrease in the synergistic effect between components, leading to a decrease in the whitening, moisturizing, and barrier repair effects of the skincare products. In Comparative Example 8, commercially available oxidized resveratrol encapsulation was used, and in Comparative Example 9, unencapsulated oxidized resveratrol powder was used. Both were inferior to the oxidized resveratrol microspheres of this invention, and the whitening, moisturizing, and barrier repair effects of the skincare products were inferior to those of Example 1. Comparative Example 10 used Rhodiola rosea extract to replace loquat leaf extract, Comparative Example 11 used aloe vera extract to replace Phalaenopsis orchid extract, and Comparative Example 12 used seaweed extract to replace Hibiscus rosa-sinensis extract. These ingredients could not be well combined and synergistic with the other components, resulting in poor synergistic effects among the components and reduced whitening, moisturizing and barrier repair effects of the skin care products.

[0112] Example 4: Testing on users with sensitive skin

[0113] Questionnaire: Thirty Asian subjects with sensitive facial skin (positive lactic acid sting test) and recent symptoms of sensitive skin were selected to participate in the test. They were given the skincare product described in Example 1, and each subject was given 2 mg / cm³. 2 A suitable amount of the product was applied to the face and a 28-day usage test was conducted. Consumers completed a questionnaire survey on the degree of improvement in indicators such as user experience, discomfort, tolerance, and gentleness without irritation. The test results are shown in Table 6.

[0114] Table 6. Results of a consumer survey on the skincare products for sensitive skin in Example 1.

[0115]

[0116]

[0117] As can be seen from Table 6, the skin care product of Example 1 of the present invention is suitable for people with sensitive skin, can meet the whitening, spot removal and moisturizing needs of people with sensitive skin, and is non-irritating.

[0118] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A whitening and spot-removing composition for sensitive skin, characterized in that, It includes the following components: oxidized resveratrol microspheres, acetyl hexapeptide-1, loquat leaf extract, Phalaenopsis orchid extract, and Hibiscus rosa-sinensis extract; the mass ratio of the oxidized resveratrol microspheres, acetyl hexapeptide-1, loquat leaf extract, Phalaenopsis orchid extract, and Hibiscus rosa-sinensis extract is (1-5):(0.01-0.2):(0.1-1):(1-3):(0.5-1.5).

2. The whitening and freckle-removing composition for sensitive skin according to claim 1, characterized in that, The mass ratio of the oxidized resveratrol microspheres, acetyl hexapeptide-1, loquat leaf extract, Phalaenopsis orchid extract, and hibiscus extract is (1-3):(0.01-0.1):(0.1-0.5):(1-2):

1.

3. The whitening and spot-removing composition for sensitive skin according to claim 1, characterized in that, The hibiscus extract includes at least one of hibiscus flower extract and hibiscus leaf extract.

4. The whitening and freckle-removing composition for sensitive skin according to claim 1, characterized in that, The hibiscus extract includes hibiscus flower extract and hibiscus leaf extract, wherein the mass ratio of the hibiscus flower extract to the hibiscus leaf extract is (1-2):

1.

5. The whitening and spot-removing composition for sensitive skin according to claim 1, characterized in that, The oxidized resveratrol microspheres include a shell and a capsule encapsulated inside the shell; The outer shell comprises calcium ion crosslinked sodium alginate hydrogel, graphene oxide, and cellulose; The contents include oxidized resveratrol inclusions and water. The oxidized resveratrol inclusions include oxidized resveratrol and a long-chain macromolecular alcohol encapsulated around the oxidized resveratrol. The long-chain macromolecular alcohol includes at least one of 1,8-octanediol and 1,2-octanediol. The mass ratio of the oxidized resveratrol inclusion, the calcium ion crosslinked sodium alginate hydrogel, the oxidized graphene, and the water is (2.5~10)g:(0.5~60)mg:(0.15~300)mg:1g; The mass ratio of the oxidized resveratrol inclusion to the long-chain macromolecular alcohol is (4~7):(4~7); The mass ratio of the calcium ion-crosslinked sodium alginate hydrogel to the cellulose is (0.1~1):(0.1~0.8).

6. The method for preparing the whitening and spot-removing composition for sensitive skin according to any one of claims 1-5, characterized in that, The process includes the following steps: mixing oxidized resveratrol microspheres, acetyl hexapeptide-1, loquat leaf extract, phalaenopsis orchid extract, and hibiscus extract in the stated mass ratio to obtain the whitening and spot-removing composition for sensitive skin. The preparation method of the oxidized resveratrol microspheres includes the following steps: (1) Dissolve oxidized resveratrol and long-chain macromolecular alcohol in a good solvent to prepare a first solution; add an antisolvent to the first solution to cause the long-chain macromolecular alcohol to encapsulate the oxidized resveratrol and precipitate out, thereby obtaining oxidized resveratrol inclusions; (2) The encapsulated body obtained in step (1) is dispersed in water as the inner phase liquid, and sodium alginate, graphene oxide and cellulose are dispersed in water as the outer phase liquid. Microdroplets are prepared by coaxial flow gas jet technology. The outside of the microdroplet is the outer phase liquid and the inside of the microdroplet is the inner phase liquid. The outside of the microdroplet encapsulates the inside of the microdroplet. The microdroplet is then dropped into a receiving liquid containing calcium chloride to obtain the oxidized resveratrol microspheres.

7. The method for preparing the whitening and freckle-removing composition for sensitive skin according to claim 6, characterized in that, At least one of the following must be met: (1-1) The method for preparing the loquat leaf extract includes the following steps: sieving dried and pulverized loquat leaves, mixing them with propylene glycol solution, ultrasonically extracting, centrifuging, and filtering to obtain the loquat leaf extract. (1-2) The preparation method of the Phalaenopsis orchid extract includes the following steps: sieving the dried and pulverized Phalaenopsis orchid flowers, mixing them with water, ultrasonically extracting them, centrifuging and filtering them to obtain the Phalaenopsis orchid extract; (1-3) The preparation method of the hibiscus extract includes the following steps: sieving the dried and pulverized hibiscus, mixing it with an ethanol solution, ultrasonically extracting, filtering, and concentrating to obtain the hibiscus leaf extract.

8. The method for preparing the whitening and freckle-removing composition for sensitive skin according to claim 6, characterized in that, In step (2), in the external phase liquid, the mass ratio of sodium alginate to water is (0.5~2) mg:1g, and the mass ratio of graphene oxide to water is (0.3~5) mg:1g; and / or, in the receiving liquid containing calcium chloride, the concentration of calcium chloride is (2~15) mg / mL.

9. The use of the whitening and freckle-removing composition for sensitive skin according to any one of claims 1-5, and the whitening and freckle-removing composition for sensitive skin prepared by the preparation method according to any one of claims 6-8, in the preparation of cosmetics.

10. A skincare product comprising the whitening and spot-removing composition for sensitive skin as described in any one of claims 1-5, characterized in that, The whitening and spot-removing composition for sensitive skin is present in the skin care product at a mass percentage of 3%-10%.

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