A skin care composition for balancing the water-oil of skin and anti-inflammatory and its application

Abstract

The present application relates to the field of cosmetic technology, especially to the field of IPC A61K8, further, it relates to a skin care composition for balancing skin water and oil and anti-inflammatory and application thereof. The raw materials at least include at least two of Nymphaeaceae extract, Bombaxaceae extract and Pseudoalteromonas ferment extract. The composition prepared by the present application not only can improve the oil control effect of the composition, but also has good moisturizing and anti-inflammatory performance, so that the skin achieves water and oil balance; in addition, it also has good stability.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetic technology, in particular to the field of IPC A61K8, and further relates to a skin care composition for balancing skin water and oil and anti-inflammatory and application thereof. BACKGROUND

[0002] In recent years, with the improvement of people's living standards, more and more people begin to pay attention to personal image. When the skin is in a healthy state of not oily and not dry, not only will not appear serious oil, let people "oil face", but also can avoid acne, blackheads, pigmentation, large pores, dull and dry skin, peeling, allergic redness and other problems.

[0003] At present, there are many commodities on the market for controlling the oil phenomenon of the skin, such as makeup primer, loose powder, powder cake, spray, etc., but these products are mostly through adsorbing the oil on the surface of the skin to achieve the effect of controlling oil, which cannot fundamentally reduce the secretion of sebaceous glands, is "affecting the symptoms but not the root cause", at the same time, these commodities with adsorption function may cause pore blockage, acne, acne and other skin problems due to long residual time.

[0004] Chinese patent CN202011358821 discloses a refreshing oil control skin care composition and a preparation method thereof. According to the weight fraction, it includes 5-10 parts of propylene glycol, 0.1-1 part of panthenol, 0.1-1.5 parts of salicylic acid, 0.5-1 part of hamamelis virginiana extract, and 0.5-1 part of calendula extract. The technical solution is to smooth the skin by salicylic acid in the first step, dredge the blocked pores, discharge the oil in the hair follicle, help soften the cutin, make the cell metabolism normal, and effectively help the old and waste cutin to peel off. In the second step, the plant extract of calendula and hamamelis virginiana is extracted to regulate the secretion of facial oil and improve the phenomenon of skin allergy. In the third step, panthenol and propylene glycol soothe and calm the skin and improve the moisture retention of the skin. However, the salicylic acid added in the technical solution is not suitable for all skin types and may cause allergic symptoms. SUMMARY

[0005] The present application provides a skin care composition for balancing skin water and oil and anti-inflammatory in the first aspect, which comprises at least two of Nymphaeaceae extract, Bombaxaceae extract and Pseudoalteromonas ferment extract.

[0006] In some preferred embodiments, the Nymphaeaceae extract is at least one selected from Nymphaeaceae Nelumbo extract and Nymphaeaceae Nymphaea extract.

[0007] Preferably, the Nymphaeaceae extract is selected from Nymphaeaceae Nelumbo extract.

[0008] Preferably, the Nymphaeaceae Nelumbo extract is Nelumbo nucifera extract.

[0009] Preferably, the preparation method of the lotus extract comprises the following steps:

[0010] (1) fresh lotus flowers are washed, and then the lotus flowers, an extraction agent and water are mixed and crushed to obtain a lotus paste;

[0011] (2) the lotus paste is extracted to obtain an extraction solution;

[0012] (3) the extraction solution is sequentially subjected to filtration, desalination, decolorization and sterilization treatment, and then obtained.

[0013] Preferably, the mass ratio of the lotus flowers to the extraction agent is (4-5):1.

[0014] Preferably, the mass ratio of the lotus flowers to water is (3-5):1.

[0015] Preferably, the extraction agent is glycerol, propylene glycol and water.

[0016] Preferably, the mass ratio of the glycerol, propylene glycol and water is (4-8):(4-8):(75-95).

[0017] Preferably, the extraction temperature is 20-25°C, and the time is 120-480 min.

[0018] In some preferred embodiments, the Kapok family extract is a Bombax extract.

[0019] Preferably, the Bombax extract is at least one of a Bombax ceiba L. extract and a Bombax insigne Wall. extract.

[0020] Preferably, the Bombax extract is a Bombax ceiba L. extract.

[0021] Preferably, the preparation method of the Bombax ceiba L. extract comprises the following steps:

[0022] (1) fresh pollen of Bombax ceiba L. flowers is selected, and then the pollen is broken and defatted, and then 10-20 times of deionized water is added, and an alkaline agent is used to adjust the pH value to 7-9;

[0023] (2) a protease is added for enzymatic reaction, and then the filtrate is obtained by filtering while hot, and then sterilized to obtain the Bombax ceiba L. extract.

[0024] Preferably, the alkaline agent is at least one of an alkali metal hydroxide, an alkali metal carbonate and an alkali metal bicarbonate.

[0025] Preferably, the alkaline agent is an alkali metal hydroxide.

[0026] Preferably, the alkali metal hydroxide is selected from at least one of sodium hydroxide, potassium hydroxide.

[0027] Preferably, the protease is selected from at least one of neutral protease, papain, trypsin, alkaline protease.

[0028] Preferably, the protease is alkaline protease.

[0029] Preferably, the enzymatic reaction specifically includes: first incubation and stirring at 30-50℃ for 2-3h, then continue to react for 4.5-5.5h, and then increase the temperature to 65-70℃, and continue to react for 8-12min.

[0030] In some preferred embodiments, the method for preparing the Pseudoalteromonas ferment product extract includes the following steps:

[0031] The Pseudoalteromonas is cultured in a reactor to obtain a fermentation broth; the fermentation broth is diluted 800-1000 times with deionized water, and then heated at 20-70℃; the mutant strain is removed by filtration, and the supernatant 1 is obtained by centrifugation; anhydrous ethanol is added, and the mixture is treated at a low temperature of 5℃; the mixture is centrifuged at a high speed, and the supernatant 2 is removed; the precipitate is freeze-dried, dissolved, and then purified by column chromatography; glycerol is added, and the Pseudoalteromonas ferment product extract is obtained.

[0032] Preferably, the mass ratio of the supernatant 1 to the anhydrous ethanol is 1:(1-3).

[0033] Preferably, the mass ratio of the glycerol to the purified substance is (2-4):1.

[0034] In some preferred embodiments, the skin care composition includes lotus extract, kapok extract, and Pseudoalteromonas ferment product extract.

[0035] Preferably, the mass ratio of the lotus extract, the kapok extract, and the Pseudoalteromonas ferment product extract is (1-3):(1-5):(1-5).

[0036] Preferably, the mass ratio of the lotus extract, the kapok extract, and the Pseudoalteromonas ferment product extract is (2-3):(2-3):(1-4).

[0037] Preferably, the mass ratio of the lotus extract, the kapok extract, and the Pseudoalteromonas ferment product extract is 3:2:1, 2:3:2, or 2:3:4.

[0038] The applicant found through a large number of creative experiments that when the skin care composition comprises at least two of the lotus extract, the kapok extract and the Pseudoalteromonas ferment extract, the composition can inhibit the secretion of sebum to a certain extent, but the effect is not ideal. The applicant unexpectedly found that when the lotus extract is the lotus flower extract and the kapok extract is the kapok flower extract, and the composition comprises the lotus flower extract, the kapok flower extract and the Pseudoalteromonas ferment extract, the composition can further inhibit the secretion of sebum. Generally, the Pseudoalteromonas ferment extract has a good effect of reducing the differentiation of sebaceous gland cells, and the more the Pseudoalteromonas ferment extract is added, the better. However, in the system of the present application, when the Pseudoalteromonas ferment extract is added in a large amount, not only the oil control effect of the composition is not improved, but also the stability of the composition is reduced. Only when the mass ratio of the lotus flower extract, the kapok flower extract and the Pseudoalteromonas ferment extract is (2-3):(2-3):(1-4), the composition can have both the oil control effect and the good stability. The applicant speculates that this may be because: the various bioactive components contained in the lotus flower extract and the kapok flower extract in the system of the present application can reduce the expression of the key protein LEPTIN (leptin) receptor in the process of reducing the synthesis of sebum and the formation of inflammatory damage, reduce the generation of sebum and inhibit the expression of inflammatory factors, and at the same time, promote the synthesis of aquaporin 3 and hyaluronic acid, slow down the maturation of sebaceous gland cells, limit the amount of porphyrin, and achieve the dual effects of moisturizing and oil control. The Pseudoalteromonas ferment extract is a natural polymer with high molecular weight, and when it is added in a large amount, it may inhibit the action of the lotus flower extract and the kapok flower extract to a certain extent due to its complex polymer chain. At the same time, the large amount of the Pseudoalteromonas ferment extract may affect the compatibility between the active ingredients in the lotus flower extract and the kapok flower extract and the Pseudoalteromonas ferment extract, resulting in the stability being affected. The applicant further found that when the mass ratio of the lotus flower extract, the kapok flower extract and the Pseudoalteromonas ferment extract is 3:2:1 or 2:3:2 or 2:3:4, the obtained composition not only has a good oil control effect, but also has good moisturizing and anti-inflammatory properties, so that the skin achieves water and oil balance.

[0039] Preferably, the method for preparing the skin care composition for balancing the water and oil of the skin and resisting inflammation comprises the following steps: adding the Pseudoalteromonas ferment extract and the kapok flower extract into the lotus flower extract in a mass ratio, and mixing to obtain the composition.

[0040] The second aspect of the present application provides an application of a skin care composition for balancing skin water and oil, anti-inflammatory, for preparing a serum; the raw materials of the serum include, by mass percentage: 2-10% moisturizer, 5-10% skin care composition, 0.2-1% skin conditioner, 0.1-0.3% pH regulator, 0.1-0.3% thickener, 0.1-0.5% preservative, 0.1-0.5% surfactant, 2-4% solvent, and deionized water to make up the balance.

[0041] Preferably, the moisturizer is selected from one or more combinations of glycerol, 1,3-propanediol, butylene glycol, and 1,2-pentanediol.

[0042] Preferably, the moisturizer is selected from a combination of glycerol and 1,3-propanediol.

[0043] Preferably, the mass ratio of glycerol and 1,3-propanediol is (4-6):(1-2).

[0044] Preferably, the skin conditioner is selected from one or more combinations of dipotassium glycyrrhizate, trehalose, panthenol, hyaluronic acid, and collagen.

[0045] Preferably, the skin conditioner is selected from a combination of dipotassium glycyrrhizate, trehalose, and panthenol.

[0046] Preferably, the mass ratio of dipotassium glycyrrhizate, trehalose, and panthenol is (0.03-0.06):(0.1-0.3):(0.4-0.6).

[0047] Preferably, the pH regulator is at least one of arginine, citric acid, and sodium citrate.

[0048] Preferably, the surfactant is a non-ionic surfactant.

[0049] Preferably, the non-ionic surfactant is at least one of octyldodecyl polyether-16, octyldodecyl polyether-16, cetyl alcohol polyether-15, cetyl alcohol polyether-17, and cetyl alcohol polyether-20.

[0050] Preferably, the non-ionic surfactant is octyldodecyl polyether-16.

[0051] Preferably, the solvent is dipropylene glycol.

[0052] Preferably, the preparation method of the serum is:

[0053] (1) 15-25 wt% deionized water and thickener are stirred at a stirring paddle speed of 600-800 r / min for 3-5 min to obtain a mixture 1;

[0054] (2) keep the remaining deionized water, humectant, skin conditioning agent and pH adjuster at 75-80℃ for 5 min, then stir at 2500-3500 r / min for 10-20 min to obtain mixture 2;

[0055] (3) when mixture 2 is cooled to 50-60℃, mixture 1 is added, and homogenized at 3000-3500 r / min for 5-7 min to obtain mixture 3;

[0056] (4) when mixture 3 is cooled to 30-40℃, preservative, surfactant and solvent are added, and after stirring uniformly, skin care composition is continuously added, and homogenized at 3000-3500 r / min for 3-5 min to obtain mixture 4;

[0057] (5) after filtering and defoaming mixture 4, it is obtained.

[0058] Beneficial effects:

[0059] 1. When the Nelumbonaceae extract is lotus flower extract, the Bombaxaceae extract is cotton flower extract, and the composition simultaneously comprises lotus flower extract, cotton flower extract and Pseudoalteromonas ferment extract, the sebum secretion can be further inhibited.

[0060] 2. When the mass ratio of lotus flower extract, cotton flower extract and Pseudoalteromonas ferment extract is (2-3) :(2-3) :(1-4), the composition not only has good oil control effect, but also has good stability.

[0061] 3. When the mass ratio of lotus flower extract, cotton flower extract and Pseudoalteromonas ferment extract is 3:2:1 or 2:3:2 or 2:3:4, the composition not only has good oil control effect, but also has good moisturizing and anti-inflammatory properties, so that the skin achieves water and oil balance.

[0062] 4. When the essence liquid contains 5-10% skin care composition, the obtained essence liquid not only has excellent oil control effect, but also has good stability.

[0063] 5. The use of glycerol, propylene glycol and water as extractant can not only improve the solubility of active ingredients such as flavonoids, alkaloids, polysaccharides and triterpenes in lotus flower extract, increase the content of active ingredients, but also avoid the precipitation of impurities, and improve the stability of active ingredients in lotus flower extract; the use of specific protease and enzymolysis conditions can improve the content and stability of active ingredients in cotton flower extract. BRIEF DESCRIPTION OF DRAWINGS

[0064] Figures 1-6The photos of the compositions prepared by the present application embodiments 1-6 respectively are placed at-4℃, 25℃, light, 45℃ for one month respectively. DETAILED DESCRIPTION

[0065] Embodiment 1

[0066] Embodiment 1 provides a skin care composition for balancing skin oil and water, anti-inflammatory, raw materials including lotus extract, kapok flower extract and pseudomonas alternaria ferment extract.

[0067] The mass ratio of the lotus extract, kapok flower extract and pseudomonas alternaria ferment extract is 2:3:4.

[0068] The preparation method of the lotus extract comprises the following steps:

[0069] (1) fresh lotus is washed, then the lotus, extractant and water are mixed and crushed to obtain lotus paste;

[0070] (2) the lotus paste is extracted to obtain an extract;

[0071] (3) the extract is sequentially subjected to filtration, desalination, decolorization and sterilization treatment, and then obtained.

[0072] The mass ratio of the lotus and the extractant is 5:1.

[0073] The mass ratio of the lotus and water is 4:1.

[0074] The extractant is glycerol, propylene glycol and water; the mass ratio of the glycerol, propylene glycol and water is 5:5:85.

[0075] The extraction temperature is 25℃, and the time is 360min.

[0076] The preparation method of the kapok flower extract comprises the following steps:

[0077] (1) fresh kapok pollen is selected, the wall is broken and the fat is removed, then 15 times of deionized water is added, and the pH value is adjusted to 8 by potassium hydroxide;

[0078] (2) alkaline protease is added for enzymatic reaction, the filtrate is obtained by filtering while hot after the reaction is completed, and then sterilized to obtain the kapok flower extract.

[0079] The enzymatic reaction specifically comprises: first, stirring at 45℃ for 2.5h, then continuing to react for 5h, then increasing the temperature to 70℃, and continuing to react for 10min.

[0080] The preparation method of the pseudomonas alternaria ferment extract comprises the following steps:

[0081] The Pseudoalteromonas sp. is cultured in a reactor to obtain a fermentation liquor; the fermentation liquor is diluted 900 times with deionized water and heated at 50℃; the mutant strain is removed by filtration, and the supernatant 1 is obtained by centrifugation; anhydrous ethanol is added, and the mixture is treated at a low temperature of 5℃; the supernatant 2 is removed after high-speed centrifugation; the precipitate is freeze-dried, dissolved, and subjected to column chromatography for fractionation and purification, and glycerol is added to obtain the product.

[0082] The mass ratio of the supernatant 1 to the anhydrous ethanol is 1:2.

[0083] The mass ratio of the glycerol to the purified product is 3:1.

[0084] The preparation method of the skin care composition for balancing the water and oil of the skin and resisting inflammation comprises the following steps: adding the Pseudoalteromonas sp. fermentation product extract, the cotton flower extract, and the lotus flower extract into the lotus flower extract in a mass ratio, and mixing to obtain the product.

[0085] The application of a skin care composition is used to prepare an essence; the essence comprises the following raw materials in percentage by mass: 6.5% of a moisturizer, 9% of a skin care composition, 0.75% of a skin conditioner, 0.2% of a pH regulator, 0.2% of a thickening agent, 0.4% of a preservative, 0.3% of a surfactant, 2.5% of a solvent, and deionized water to make up the balance.

[0086] The moisturizer is selected from the combination of glycerol and 1,3-propanediol.

[0087] The mass ratio of the glycerol to the 1,3-propanediol is 5:1.5.

[0088] The skin conditioner is selected from the combination of dipotassium glycyrrhizinate, trehalose, and panthenol.

[0089] The mass ratio of the dipotassium glycyrrhizinate, the trehalose, and the panthenol is 0.05:0.2:0.5.

[0090] The pH regulator is arginine.

[0091] The surfactant is octyldodecyl polyoxyl-16.

[0092] The solvent is dipropylene glycol.

[0093] The preparation method of the essence comprises the following steps:

[0094] (1) 20wt% of deionized water and a thickening agent are stirred at a stirring paddle speed of 700 / min for 4 min to obtain a mixture 1;

[0095] (2) the remaining deionized water, the moisturizer, the skin conditioner, and the pH regulator are kept at 80℃ for 5 min, and then stirred at 3000r / min for 15 min to obtain a mixture 2;

[0096] (3) When mixture 2 is cooled to 55℃, mixture 1 is added, and homogenized at 3500 r / min for 6 min to obtain mixture 3;

[0097] (4) When mixture 3 is cooled to 35℃, preservatives, surfactants and solvents are added, and after stirring, skin care compositions are continuously added, and homogenized at 3500 r / min for 4 min to obtain mixture 4;

[0098] (5) After mixture 4 is filtered and defoamed, it is obtained.

[0099] Example 2

[0100] Example 2 provides a skin care composition for balancing skin water and oil and anti-inflammatory, which has the same specific implementation as Example 1, except that the mass ratio of lotus extract, kapok flower extract and pseudomonas ferment extract is 2:3:2.

[0101] Example 3

[0102] Example 3 provides a skin care composition for balancing skin water and oil and anti-inflammatory, which has the same specific implementation as Example 1, except that the mass ratio of lotus extract, kapok flower extract and pseudomonas ferment extract is 3:2:1.

[0103] Example 4

[0104] Example 4 provides a skin care composition for balancing skin water and oil and anti-inflammatory, which has the same specific implementation as Example 1, except that the mass ratio of lotus extract, kapok flower extract and pseudomonas ferment extract is 2:2:1.

[0105] Example 5

[0106] Example 5 provides a skin care composition for balancing skin water and oil and anti-inflammatory, which has the same specific implementation as Example 1, except that the mass ratio of lotus extract, kapok flower extract and pseudomonas ferment extract is 3:3:1.

[0107] Example 6

[0108] Example 6 provides a skin care composition for balancing skin water and oil and anti-inflammatory, which has the same specific implementation as Example 1, except that the mass ratio of lotus extract, kapok flower extract and pseudomonas ferment extract is 2:3:5.

[0109] Example 7

[0110] Example 7 provides a skin care composition for balancing the skin's water-oil balance, anti-inflammatory, having the same specific embodiment as Example 1, except that the Lotus extract, Kapok flower extract, and Pseudoalteromonas ferment extract are present in a mass ratio of 3:4:1.

[0111] Example 8

[0112] Example 8 provides a skin care composition for balancing the skin's water-oil balance, anti-inflammatory, having the same specific embodiment as Example 1, except that the Lotus extract, Kapok flower extract, and Pseudoalteromonas ferment extract are present in a mass ratio of 3:5:1.

[0113] Comparative Example 1

[0114] Comparative Example 1 provides a skin care composition for balancing the skin's water-oil balance, anti-inflammatory, having the same specific embodiment as Example 1, except that the Lotus extract, Kapok flower extract, and Pseudoalteromonas ferment extract are present in a mass ratio of 1:0:0.

[0115] Comparative Example 2

[0116] Comparative Example 2 provides a skin care composition for balancing the skin's water-oil balance, anti-inflammatory, having the same specific embodiment as Example 1, except that the Lotus extract, Kapok flower extract, and Pseudoalteromonas ferment extract are present in a mass ratio of 0:1:0.

[0117] Comparative Example 3

[0118] Comparative Example 3 provides a skin care composition for balancing the skin's water-oil balance, anti-inflammatory, having the same specific embodiment as Example 1, except that the Lotus extract, Kapok flower extract, and Pseudoalteromonas ferment extract are present in a mass ratio of 0:0:10.

[0119] Comparative Example 4

[0120] Comparative Example 4 provides a skin care composition for balancing the skin's water-oil balance, anti-inflammatory, having the same specific embodiment as Example 1, except that the Lotus extract, Kapok flower extract, and Pseudoalteromonas ferment extract are present in a mass ratio of 1:1:0.

[0121] Comparative Example 5

[0122] Comparative Example 5 provides a skin care composition for balancing the skin's water-oil balance, anti-inflammatory, having the same specific embodiment as Example 1, except that the Lotus extract, Kapok flower extract, and Pseudoalteromonas ferment extract are present in a mass ratio of 0:1:1.

[0123] Comparative Example 6

[0124] Comparative Example 6 provides a skin care composition for balancing the water and oil of the skin, anti-inflammatory, the specific embodiment of Example 1, the difference is that the mass ratio of the lotus extract, kapok flower extract and pseudomonas caenopora fermentation product extract is 1:0:1.

[0125] Performance test:

[0126] I. For the composition:

[0127] 1. Cytotoxicity evaluation experiment:

[0128] Principle: MTT assay is by the number of living cells in the detection of cell toxicity or cell proliferation, widely used in experimental methods, its detection principle is that the mitochondrial succinate dehydrogenase in the living cells can make water-soluble yellow salt MTT (3-(4, 5-dimethylthiazole-2)-2, 5-diphenyl tetrazolium bromide) reduced to water-insoluble blue formazan and deposited in the cells, while dead cells have no such function. The generated crystalline formazan is generally added with DMSO (dimethyl sulfoxide), and the absorbance is measured after dissolution.

[0129] Experimental method: In 96 multi-well plates, 100 μl of DMEM medium containing 10% bovine serum and keratinocytes (HaCaT) were inoculated at a density of 1X104 cells / well, and cultured for 24 hours. After 24 hours, the medium was replaced with serum-free medium. After 24 hours of treatment in serum-free medium, the medium was removed and 20 μL of MTT solution was added for treatment, and the reaction was allowed to proceed at 37°C for 2 hours. 200 μL of isopropyl alcohol was added to the MTT solution in the cells, and gently shaken for 30 min to completely dissolve the crystalline formazan. The absorbance was measured at 570 nm, and the cell survival rate was calculated according to the following formula.

[0130]

[0131] The blank group does not add the composition for testing, and the results are shown in Table 1.

[0132] 2. Oil control effect:

[0133] Select in vitro cultured SZ95 human sebaceous gland cells, the cells are cultured in Sebomed base medium, adding 10% fetal bovine serum, 5 μg / L epidermal growth factor, 100 U / mL penicillin, 100 μg / mL streptomycin. Placed in a 37℃, 5%, CO2 incubator, the culture medium is replaced every 2 days. Cells are inoculated in a 96-well plate, the next day the same mass of composition is added, after 48h of incubation, the supernatant is discarded, washed twice with PBS, repeatedly sucked and beaten, then divided into two parts, one part is added with Nile red dye (1 mg / ml, dissolved in acetone, -20℃ storage) diluted with PBS to a final concentration of 10 μg / mL, the other part is added with fluorescein diacetate (FDA) (5 mg / mL dissolved in DMSO, -20℃ storage) diluted with PBS to a final concentration of 15 μg / mL. The fluorescence intensity of different samples is detected, Nile red is excited at 485 nm, and the emission wavelength is detected at 565 nm, FDA is excited at 494 nm, and the emission wavelength is detected at 523 nm, and the calculation is as follows: intracellular neutral lipid percentage (%) = Nile red fluorescence intensity / FDA fluorescence intensity x 100%; the lower the intracellular neutral lipid percentage, the better the oil control effect, and the results are shown in Table 2.

[0134] 3. Moisturizing effect:

[0135] Principle: Real-time fluorescent quantitative PCR is a method of measuring the total amount of product after each polymerase chain reaction (PCR) cycle in DNA amplification reaction with fluorescent chemicals. The method quantitatively analyzes the expression of specific genes in the sample to be tested by internal or external reference method. By comparing the experimental group and the control group, it can be analyzed whether the expression of specific genes changes after being treated by a certain sample, and then it can be inferred whether it has a potential effect on the function of gene-related proteins.

[0136] Experimental method: HaCaT cells are inoculated in a 6-well plate at a density of 3x105 / well, 3 parallel holes are plated for each sample (including negative control), and the culture medium is high-sugar DMEM + 10% FBS. Put into a constant temperature incubator, culture conditions are 37 degrees Celsius, 5% CO2, 20-24 hours later, replace with culture medium containing 0.65% sample, negative control is DMEM medium containing 10% FBS, culture conditions are 37 degrees Celsius, 5% CO2. After 24 hours of culture, rinse twice with 1xPBS, 1 mL each time, then add 1 mL of TRIzol, blow 15 times with 1 mL of RNase-free gun head, then transfer to a 1.5 mL RNase-free Eppendorf tube, and freeze in a ultra-low temperature freezer at negative 80℃. After 24 hours, the sample is detected by real-time fluorescent quantitative PCR, the detection gene is AQP-3, and the gene expression analysis is carried out by 2-ΔΔCt method. The higher the gene expression of water channel protein AQP-3, the better the moisturizing effect, and the results are shown in Table 3.

[0137] 4. Anti-inflammatory effect: The anti-inflammatory effect of the composition was determined according to the standard "T / SHRH 034-2021 Cosmetic Soothing Effect Test - In Vitro TNF-α Inflammatory Factor Content Determination Lipopolysaccharide Induced Macrophage RAW264.7 Test Method".

[0138] Principle: By determining the difference in TNF-α content after the action of different test substances, the effect of the test substance on inhibiting the secretion of TNF-α is evaluated. The specific evaluation indexes are TNF-α content and TNF-α inhibition rate, wherein the TNF-α content is calculated after being determined by enzyme-linked immunoassay (ELISA), and the average value of 3 replicate wells of each group is taken as the final TNF-α content result; the calculation formula of TNF-α inhibition rate is as follows:

[0139] TNF-α inhibition rate (%) = (1 - test substance TNF-α content average value / negative control TNF-α content average value) x 100%.

[0140] Experimental method: inoculate macrophages into a 12-well plate, incubate in a incubator at 37°C, 5% CO2 aeration amount for 24h, add 1% diluent directly diluted with culture medium, add LPS (1 μg / mL) after 2h, set up negative control group containing 1 μg / mL LPS, positive control group containing 100 μg / mL dexamethasone and LPS cell culture medium, stimulate for 24h, collect supernatant, centrifuge, and detect. The ELISA kit is used to analyze the release level of pro-inflammatory inflammatory factor TNF-α of RAW264.7. The higher the TNF-α inhibition rate, the better the anti-inflammatory effect of the multiple strain fermentation filtrate, and the results are shown in Table 4.

[0141] 5. Stability: Test conditions and results are shown in Table 5.

[0142] II. For serum:

[0143] 1. Skin oil content value:

[0144] Principle: The probe of the skin oil content tester (Sebumeter SM815) is based on the principle of a photometer. A special extinction tape 0.1 mm thick absorbs the oil on the human skin and becomes a kind of translucent tape. The light transmittance of the tape will change, the more oil absorbed, the greater the light transmittance, so the content of skin oil can be measured.

[0145] Unit: μg sebum / cm 2 .

[0146] Equipment and instruments: skin oil content tester Sebumeter SM815 (Germany CK Company).

[0147] Test method:

[0148] (1) Select 30 subjects with oily skin, male and female, aged 18-50 (pregnant and lactating women are excluded)

[0149] (2) The subjects do not use any cosmetics on the day of the test, and the face needs to be cleaned uniformly before the test. Before the formal test, sit in a standard room for at least 20 minutes and keep relaxed;

[0150] (3) Select 3 points on the left and right forehead to measure the initial value of skin oil

[0151] (4) Use the product, uniformly use the serum prepared in Example 1 on the left face, and use a commercially available oil control product (use a pipette for quantitative) on the right face.

[0152] (5) At 2h, 4h, 8h, let the subjects rest in the laboratory with temperature 21±1℃ and relative humidity 50±5% for 20min, then select points 1, 2, 3 on the left and right forehead to measure the skin oil content.

[0153] The change of oil content reflects the change of oil content in the experimental area over time during the test period. The greater the value, the greater the oil content, and vice versa. The smaller the oil content, the results are shown in Table 6.

[0154] 2, Stability: test conditions and results are shown in Table 7.

[0155] Test results:

[0156] Table 1

[0157]

[0158] Table 2

[0159] Neutral lipid percentage Example 1 64.67 Example 2 65.17 Example 3 68.54 Example 4 80.77 Example 5 71.64 Example 6 81.66 Example 7 73.68 Example 8 77.12 Comparative Example 1 95.20 Comparative Example 2 94.70 Comparative Example 3 94.48 Comparative Example 4 90.11 Comparative Example 5 91.78 Comparative Example 6 91.24

[0160] Table 3

[0161] Negative control Example 1 Example 2 Example 3 AQP-3 / 53％ 48％ 40％*

[0162] " / " represents that the sample has up-regulation effect on the gene.

[0163] "*" represents t-test, and the sample has significant difference in promoting effect on mitochondrial activity compared with the negative control under the test conditions, *p<0.05.

[0164] Table 4

[0165]

[0166]

[0167] Significance is indicated by * compared to negative control group, P-value < 0.05 is indicated by *, P.value < 0.01 is indicated by **, P.value < 0.001 is indicated by ***.

[0168] Table 5

[0169] In the table: D means day, M means month;

[0170] 0-3 score appearance, odor, color;

[0171] 0 - consistent with the standard

[0172] 1 - slight change, still applicable

[0173] 2 - moderate change, not acceptable

[0174] 3 - significant change

[0175] Appearance (A:Appearance) / Fragrance (F:Fragarance / Odour) / Color (C:Color)

[0176]

[0177]

[0178] Table 6

[0179]

[0180] Table 7

[0181] Test sample: serum in Example 1;

[0182] In the table: W means week, M means month;

[0183] 0-3 score appearance, odor, color;

[0184] 0 - consistent with the standard

[0185] 1 - slight change, still applicable

[0186] 2 - moderate change, not acceptable

[0187] 3 - significant change

[0188] Appearance (A:Appearance) / Fragrance (F:Fragarance / Odour) / Color (C:Color)

[0189]

Claims

1. A skincare composition that balances the skin's oil and moisture levels and has anti-inflammatory properties, characterized in that, The skincare composition contains lotus extract, kapok flower extract, and Pseudomonas aeruginosa fermentation product extract. The preparation method of the lotus extract includes the following steps: (1) Take fresh lotus flowers, wash them, mix the lotus flowers, extractant and water, and then crush them to obtain lotus pulp; (2) Extract the lotus slurry to obtain an extract; (3) The extract is obtained by sequentially filtering, desalting, decolorizing and sterilizing; The extractant is a mixture of glycerol, propylene glycol and water; The preparation method of the kapok flower extract includes the following steps: (1) Select fresh kapok pollen, break the cell wall and defatt the pollen, add 10 to 20 times the amount of deionized water, and adjust the pH value to 7 to 9 using an alkaline agent; (2) Add protease to carry out enzymatic hydrolysis. After the reaction is complete, filter while hot and collect the filtrate. Sterilize to obtain the final product. The protease is an alkaline protease; The method for preparing the Pseudomonas alterniflora fermentation product extract includes the following steps: Marine pseudoalternating monoclonal bacteria were cultured in a reactor to obtain a fermentation broth. The fermentation broth was diluted 800 to 1000 times with deionized water and heated at 20 to 70°C. The mutant strains were removed by filtration, and the supernatant 1 was collected by centrifugation. Anhydrous ethanol was added, and the mixture was treated at 5°C. After high-speed centrifugation, the supernatant 2 was removed. The precipitate was freeze-dried, dissolved, and purified by column chromatography. Glycerol was added to obtain the final product.

2. The skincare composition according to claim 1, characterized in that, The mass ratio of the lotus extract, the kapok flower extract and the pseudoalternating monotypic fermentation product extract is (1~3):(1~5):(1~5).

3. The skincare composition according to claim 1, characterized in that, The mass ratio of the lotus extract, the kapok flower extract and the pseudoalternating monoclonal fermentation product extract is (2~3):(2~3):(1~4).

4. The skincare composition according to claim 1, characterized in that, The mass ratio of the lotus extract, the kapok flower extract, and the Pseudomonas aeruginosa fermentation product extract is 3:2:1, 2:3:2, or 2:3:

4.

5. The application of a skincare composition according to any one of claims 1 to 4, characterized in that, For preparing an essence, the essence, by weight percentage, comprises: 2-10% moisturizer, 5-10% skin care composition, 0.2-1% skin conditioning agent, 0.1-0.3% pH adjuster, 0.1-0.3% thickener, 0.1-0.5% preservative, 0.1-0.5% surfactant, 2-4% dipropylene glycol, and deionized water to make up the balance.

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