Box for preserving biological samples on the inside and outside of a condom and method for pre-treating biological samples

Abstract

The present application relates to the field of medical detection technology, and particularly relates to a box for storing biological samples on the inner and outer sides of a condom and a biological sample pretreatment method. The box comprises a box body and a box cover, the box cover can seal the box body, a sealing structure in the form of a boss is arranged in a storage space in the box body, the boss divides the storage space in the box body into two chambers, when a used condom is stored, the edge ring of the condom and the condom film part with a biological sample are located in different chambers respectively; a limiting structure is arranged inside the box cover, the limiting structure cooperates with the boss to seal the box body, when sealed, the condom film is deformed under pressure, so that the inner and outer sides of the condom film are isolated. The present application avoids cross contamination of the biological samples on the inner and outer sides of the condom during transportation. The biological sample pretreatment method comprises the following steps: storing a biological sample detection reagent in a chamber of the condom film; placing the condom into the box body; controlling the biological sample on the condom to contact the biological sample detection reagent to complete pretreatment.

Description

Technical Field

[0001] This invention relates to the field of medical testing technology, specifically to a box for storing biological samples from the inside and outside of a condom and a method for preprocessing biological samples. Background Technology

[0002] With the advancement of medical technology, IVD (in vitro diagnostics) technology has experienced rapid development in the past 20 years due to its advantages of speed, accuracy, and convenience. IVD refers to a novel diagnostic technology that provides clinical diagnostic and treatment basis by detecting biomarkers in human samples in vitro. These biomarkers can include nucleic acids, proteins, cytokines, etc. By detecting the presence and expression levels of these biomarkers, doctors can help determine the patient's disease morphology, prognosis, and drug response. Biological samples for IVD technology can be human biological samples containing biomarkers, such as blood, urine, and tissues. Male semen and female vaginal secretions can also be used as biological samples for IVD.

[0003] Condoms are widely used medical devices during sexual intercourse to prevent unintended pregnancy. During use, condoms come into contact with the sexual organs and bodily fluids of both partners. After intercourse, the condom contains shed tissue and residual bodily fluids from both individuals, which can be used as biological samples for in vitro fertilization (IVD) testing. Therefore, a used condom can be used simultaneously as a sampling device for male external genitalia shed tissue and semen, and for female vaginal shed tissue and vaginal secretions; the collected biological samples can then be used for IVD testing of both partners.

[0004] Using used condoms for simultaneous IVD testing of both men and women presents a practical problem: the separation of the sampling and testing processes. Sampling is a private matter, while testing is typically conducted in a laboratory. Therefore, using used condoms for IVD testing involves not only temporal separation but also spatial separation between the sampling and testing processes. This necessitates consideration of the preservation and transportation of biological samples. Furthermore, the potential for cross-contamination between male and female samples from the same condom must be addressed. Summary of the Invention

[0005] In order to solve the above-mentioned technical problems in the prior art, the present invention provides a box for storing biological samples from the inside and outside of condoms. This not only solves the problem of storing and transporting used condoms, but also prevents cross-contamination of biological samples between men and women during the transportation of condoms with collected biological samples.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows:

[0007] In a first aspect, the present invention provides a box for storing biological samples from the inside and outside of condoms, comprising a box body and a box lid, the box lid being capable of sealing the box body, the box body having a storage space for storing condoms, and a sealing structure being provided within the storage space of the box body; when a condom is placed in the box body and the box lid seals the box body, the sheath of the condom is subjected to pressure, isolating the inside and outside of the condom; the sealing structure is a boss, the boss dividing the storage space of the box body into two chambers, wherein when storing used condoms, the rolled edge of the condom and the sheath portion containing biological samples from the inside and outside of the condom are located in different chambers; a limiting structure is provided inside the box lid, and when the box body and the box lid are closed, the limiting structure cooperates with the boss to seal the box body, and the sheath is subjected to pressure deformation from the sealing structure of the box body and the limiting structure of the box lid, isolating the inside and outside of the sheath.

[0008] Furthermore, the sealing structure is a boss located inside the box and indirectly connected to the side wall of the box; when placing the condom, the rolled edge of the condom is placed between the boss and the side wall of the box.

[0009] Furthermore, the box body includes a bottom wall, and a side wall is provided on the outer edge of the bottom wall. An extension wall is connected to the upper end of the side wall, and the extension wall extends outward along the horizontal direction of the box body. An extension wall protrusion is provided on the upper surface of the outer edge of the extension wall. The limiting structure is a first protrusion, and a retaining ring is provided on the edge of the lower surface of the box cover. The first protrusion is embedded in the boss, and the retaining ring is engaged with the outside of the extension wall protrusion to seal the box body and the box cover.

[0010] Furthermore, the inner edge of the extended wall extends inward along the horizontal direction of the box body, and a boss is provided on the upper surface of the inner edge of the extended wall. When the box lid seals the box body, the planar distance between the side wall of the limiting structure and the inner wall of the boss is less than the thickness of the condom film. The condom film portion located between the side wall of the limiting structure and the inner wall of the boss is squeezed and deformed. The side wall of the limiting structure, the inner wall of the boss, and the condom film portion between them form a sealing surface, thereby isolating the inner and outer sides of the condom film portion.

[0011] Furthermore, when the lid seals the box body, the rolled edge of the condom is placed within the space formed by the boss, the extension wall protrusion, the extension wall, and the lid portion above the extension wall; the vertical distance between the inner wall portion of the lid that contacts the top of the rolled edge of the condom and the outer wall portion of the extension wall that contacts the bottom of the rolled edge of the condom is less than the thickness of the rolled edge of the condom, that is, the rolled edge of the condom is compressed and deformed, and the rolled edge of the condom, the inner surface portion of the lid that contacts the top of the rolled edge of the condom, and the outer surface portion of the extension wall that contacts the bottom of the rolled edge of the condom form a sealing surface to prevent the liquid inside the condom film from leaking out.

[0012] Furthermore, the box also includes a locking structure for locking the lid and the box body; the locking structure is a snap fastener, which is located below the snap ring and engages with the outer edge of the extension wall; or, the locking structure is a latch and a snap fastener, with the latch located on one side of the lid and the snap fastener located on one side of the box body, the latch engaging with the snap fastener to lock the box body and the lid.

[0013] Furthermore, the sealing structure is a boss located inside the box and directly connected to the side wall of the box.

[0014] Furthermore, a connecting wall is provided on the top of the side wall of the box body, the outer surface of the connecting wall is located inside the side wall of the box body, and a second groove is provided inside the protrusion; the limiting structure is a linear pressure structure, the linear pressure structure is connected to the side wall of the box lid, and a second protrusion is provided inside the linear pressure structure. The inner side wall of the box lid is attached to the outer surface of the connecting wall, and the second protrusion is embedded in the second groove, so that the box body and the box lid are sealed. The vertical distance between the inner wall of the bottom of the second groove and the inner wall of the top of the second protrusion is less than the thickness of the condom film, that is, the condom film portion located between the second groove and the second protrusion is squeezed and deformed. The inner wall of the bottom of the second groove, the inner wall of the top of the second protrusion, and the condom film portion between them together form a sealing surface, so that the inner and outer sides of the condom film portion are isolated.

[0015] Furthermore, the box lid is provided with at least one spike inside the cavity where the sheath portion of the condom is located. The spike is used to puncture the liquid biological sample detection reagent encapsulated in the membrane placed inside the box, and the spike does not contact the sheath of the condom.

[0016] Furthermore, a button is provided on the outside of the box cover. The button is connected to the spike. When the button is operated, the tip of the spike contacts the bottom of the chamber. When the button is not operated, the tip of the spike is not in contact with the bottom of the chamber.

[0017] Furthermore, the button and the spike are rotatably connected, and the spike is moved by rotating the button; or, the button and the spike are elastically connected, and the spike is moved by squeezing the button.

[0018] Secondly, the present invention provides a biological sample pretreatment method, comprising the following steps:

[0019] Step S1: Using the aforementioned box, pre-store the biological sample testing reagent in the cavity of the sheath portion of the condom;

[0020] Step S2: Place the condom containing the biological sample into the box;

[0021] Step S3: Control the contact between the biological samples on the inside and outside of the condom and the biological sample detection reagent to complete the pretreatment.

[0022] Furthermore, the biological sample testing reagent is stored in the form of a solid powder. The biological sample testing reagent is coated on the inner wall of the box, so that the biological sample on the outside of the condom comes into contact with the inner wall of the box to complete the pretreatment.

[0023] Furthermore, the biological sample detection reagent is a liquid form encapsulated in a membrane. By puncturing the membrane, the detection reagent flows out and comes into contact with the biological sample to complete the pretreatment.

[0024] Furthermore, the biological sample detection reagent is in the form of a hydrogel. After the hydrogel comes into contact with the biological sample, it completes the pretreatment through osmosis and diffusion.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The box for storing biological samples from the inside and outside of a condom provided by this invention divides the box into multiple sealed spaces through an internal sealing structure. A limiting structure is provided inside the lid. When the box body and lid are closed, the limiting structure and the protrusion cooperate to seal the box body. The rolled edge of the condom and the membrane portion containing the biological samples on the inside and outside of the condom are located in different chambers. The membrane is subjected to pressure deformation from the sealing structure of the box body and the limiting structure of the lid, thereby isolating the inside and outside of the condom and preventing cross-contamination of the biological samples during transportation.

[0027] In addition, biological sample detection reagents are placed inside the box, providing pretreatment measures for the detection of biological samples and overcoming the inherent defects of IVD detection methods, which separate sampling and detection in time and space. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the box after it is snapped shut according to Embodiment 1 of the present invention;

[0029] Figure 2 This is a schematic diagram of the split structure of the box after it is opened, according to Embodiment 1 of the present invention;

[0030] Figure 3 This is a schematic diagram of the box lid structure of Embodiment 1 of the present invention;

[0031] Figure 4 This is a schematic cross-sectional view of the assembly of Example 1 with a condom installed;

[0032] Figure 5 This is a schematic diagram of the box structure after it is snapped together when the box is square in Example 2.

[0033] Figure 6 In Example 2 Figure 5 A schematic diagram of the box after it has been opened;

[0034] Figure 7 This is a schematic diagram of the structure after the box is snapped together when it is hexagonal, as shown in Example 2.

[0035] Figure 8 In Example 2 Figure 7 A schematic diagram of the box after it has been opened;

[0036] Figure 9 This is a schematic diagram of the box with a linear pressure structure in Example 3, when it is filled with a condom and opened.

[0037] Figure 10 This is a schematic diagram of the open structure of the box with the linear pressure structure in Example 3;

[0038] Figure 11 This is an enlarged schematic diagram of the straight sealing surface in Example 3;

[0039] Figure 12 This is an enlarged schematic diagram of the closed end of the boss after sealing various linear types in Example 3;

[0040] Figure 13 This is a schematic diagram of the automatic puncture of the membrane sac when the box is closed in Example 4;

[0041] Figure 14 This is a schematic diagram of the manual puncture of the membrane sac after closure in Example 5.

[0042] Explanation of reference numerals in the attached figures:

[0043] 1-Lid, 2-Box body, 3-Locking structure, 4-Condom, 5-Rolled edge ring, 6-Sheet film, 7-Protrusion, 8-Extension wall, 9-Extension wall protrusion;

[0044] 10-Limiting structure, 11-Inner ring annular sealing surface, 12-Outer ring annular sealing surface, 13-Connecting wall, 14-Folded double-layer adhesive membrane, 15-Closed end, 16-Spiked part, 17-Membrane bladder, 18-Button. Detailed Implementation

[0045] The technical solution of the present invention will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are not all embodiments of the present invention. All other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention.

[0046] It should be noted that, unless otherwise specifically stated, the relative arrangement and numerical expressions of the components and steps described in these embodiments should not be construed as limiting the scope of the invention.

[0047] The following description of exemplary embodiments is merely illustrative and is not intended to limit the invention or its application or use in any way. Techniques, methods, and apparatus known to those skilled in the art may not be discussed in detail herein, but where applicable, such techniques, methods, and apparatus should be considered part of this specification.

[0048] Example 1

[0049] Combination Figure 1 , Figure 2 As shown in the figure, this embodiment provides a box for storing biological samples from the inside and outside of a condom. The basic structure of the box is as follows: the box includes a lid 1 and a body 2. The lid 1 can seal the body 2. The body 2 has a storage space for storing condoms 4. A sealing structure is provided in the storage space of the body 2.

[0050] When the condom 4 is placed inside the box 2 and the box lid 1 seals the box 2, the locked box lid 1 and box 2 apply pressure to the condom 4, isolating the inner side of the condom 4 from the outer side of the condom 4, thereby isolating the biological samples collected on the inner and outer sides of the condom 4 from each other and avoiding cross-contamination.

[0051] like Figure 2As shown, the sealing structure is a boss 7, which divides the storage space of the box 2 into two chambers. When storing used condoms 4, the rolled edge 5 of the condom and the sheath 6 of the condom with biological sample on the inner and outer sides of the condom 4 are located in different chambers.

[0052] The lid 1 is provided with a limiting structure inside. When the box body 2 and the lid 1 are closed, the limiting structure and the boss 7 cooperate with each other to seal the box body 1 with the lid 2.

[0053] In this embodiment, a circular box is used as an example for illustration. Both the box body 2 and the box lid 1 are circular.

[0054] Preferably, the boss 7 is also a circular structure.

[0055] like Figure 2 As shown, the box body 2 includes a bottom wall, and a side wall is provided on the outer edge of the bottom wall. An extension wall 8 is connected to the upper end of the side wall, and the extension wall 8 extends outward along the horizontal direction of the box body 2. An extension wall protrusion 9 is provided on the upper surface of the outer edge of the extension wall 8. A locking structure 3 is provided on the edge of the lower surface of the box cover 1. The locking structure 3 can be a retaining ring. The retaining ring is engaged with the outside of the extension wall protrusion 9 to seal the box body 2 and the box cover 1.

[0056] like Figure 3 As shown, a limiting structure 10 is provided inside the box cover 1, and the limiting structure 10 is a first protrusion;

[0057] Combination Figure 2 , 3 As shown in Figure 4, the inner edge of the extension wall 8 extends inward along the horizontal direction of the box body 2. A boss 7 is provided on the upper surface of the inner edge of the extended wall 8. That is, the boss 7 is indirectly connected to the side wall of the box body 2 through the extension wall 8. The middle part of the boss 7 is a circular through hole that communicates with the cavity inside the box body 1. When the box cover 1 seals the box body 2, the limiting structure 10 is embedded in the circular through hole of the boss 7.

[0058] The planar distance between the sidewall of the limiting structure 10 and the inner wall of the protrusion 7 is less than the thickness of the sheath 6 of the condom. The sheath portion of the condom located between the sidewall of the limiting structure 10 and the inner wall of the protrusion 7 is squeezed and deformed. The sidewall of the limiting structure 10, the inner wall of the protrusion 7, and the sheath portion of the condom between them form a sealing surface, thereby isolating the inner and outer sides of the sheath 6 of the condom 4 containing the biological sample.

[0059] Combination Figure 2 , 3As shown in Figure 4, when the box cover 1 seals the box body 2, the rolled edge 5 of the condom is placed in the space formed by the boss 7, the extension wall protrusion 9, the box cover 1, the extension wall 8, and the box cover portion above the extension wall 8.

[0060] The vertical distance between the inner wall portion of the lid 1 that contacts the top of the rolled edge 5 of the condom and the outer wall portion of the extension wall 8 that contacts the bottom of the rolled edge 5 of the condom is less than the thickness of the rolled edge 5 of the condom. That is, the rolled edge 5 of the condom is deformed by compression. The rolled edge 5 of the condom, the inner wall portion of the lid 1 that contacts the top of the rolled edge 5 of the condom, and the outer wall portion of the extension wall 8 that contacts the bottom of the rolled edge 5 of the condom form a sealing surface to prevent liquid from leaking out from the inside of the sheath 6 of the condom.

[0061] Combination Figure 2 , 3 As shown in Figure 4, the lid 1 and the body 2 are fastened together to apply and maintain pressure on the condom 4, forming an inner and outer isolation, so that the body 2 is in a sealed state, thus isolating the inner side of the condom 4 from the outer side of the condom 4, and isolating the condom 4 from the outside world.

[0062] Combination Figure 2 , 3 As shown in Figure 4, the close contact between the box lid 1, the condom 4, and the box body 2 forms four annular sealing surfaces, including an inner annular sealing surface 11 and an outer annular sealing surface 12; the boundary lines of these four annular sealing surfaces are all closed circles.

[0063] The locking structure 3 also includes a buckle, which is located below the retaining ring and is engaged with the outer edge of the extension wall 8. The retaining ring and the buckle engage to seal the box body 2 and the box lid 1.

[0064] As another embodiment of the locking structure 3, the locking structure 3 is a latch and a latch. The latch is located on one side of the lid 1 and the latch is located on one side of the box body 2. The latch and the latch engage to lock the box body 2 and the lid 1.

[0065] As another embodiment, the box in this embodiment can be a combination of the box body 2 in any form of square or polygonal structure, and the boss 7 in any form of circular, square or polygonal structure.

[0066] Of course, the box 2 can also be made into any other regular or irregular shape.

[0067] In this embodiment, after the box body 2 and the box cover 1 are sealed, the boundary lines of the pressure sealing surfaces formed are all closed circles or other closed curves.

[0068] Example 2

[0069] Combination Figure 5 , Figure 6 ,as well as Figure 7 , Figure 8 As shown, this embodiment provides a box for storing biological samples from the inside and outside of a condom. Its basic structure is the same as that of Embodiment 1, but the difference is as follows:

[0070] In this embodiment, the box body 2 has no extension wall protrusion 9 structure. In this embodiment, the side wall and the boss 7 are connected through the extension wall 8. And there is no need to set the locking structure 3. The limiting structure 10 set in the box cover 1 is the first groove.

[0071] Specifically, the outer side of the extension wall 8 is connected to the upper surface of the side wall of the box body 2, and the inner side of the extension wall 8 is connected to the boss 7. That is, the boss 7 and the side wall of the box body 2 are indirectly connected through the extension wall 8. The first groove divides the box cover 1 into two spaces. After the box cover 1 is fastened, the side wall of the first groove is fastened to the outer wall of the boss 7, and the edge of the box cover 1 is attached to the extension wall 8, so that the box body 2 and the box cover 1 are sealed.

[0072] When the lid 1 seals the box body 2, the vertical distance between the side wall of the first groove and the outer wall of the protrusion 7 is less than the thickness of the sheath 6 of the condom. The sheath portion of the condom located between the side wall of the first groove and the outer wall of the protrusion 7 is squeezed and deformed. The side wall of the first groove, the outer wall of the protrusion 7, and the sheath portion of the condom between them form a sealing surface, thereby isolating the inner and outer sides of the sheath 6 portion of the condom containing the biological sample.

[0073] When the lid 1 seals the box body 2, the rolled edge 5 of the condom is placed within the space formed by the boss 7, the lid 1, and the extension wall 8. The vertical distance between the inner wall of the lid 1 (excluding the first groove) and the outer wall of the extension wall 8 is less than the thickness of the rolled edge 5 of the condom between them. That is, the rolled edge 5 of the condom is deformed by the pressure between the inner wall of the lid 1 (excluding the first groove) and the outer wall of the extension wall 8. The rolled edge 5 of the condom, the inner wall of the lid 1 that contacts the top of the rolled edge 5 of the condom, and the outer wall of the extension wall 8 that contacts the bottom of the rolled edge 5 of the condom form a sealing surface to prevent the liquid inside the sheath 6 of the condom from leaking out.

[0074] like Figure 5 , Figure 7As shown, in this embodiment, the box body 2 is a square or polygonal structure, and the box cover 1 is a square or polygonal structure.

[0075] As another embodiment of the box body 2, the box body 2 can also be a circular structure.

[0076] The boss 7 has a square or polygonal structure.

[0077] As another embodiment of the boss 7, the boss 7 can also be a circular structure.

[0078] Therefore, in this embodiment, the box can be a combination of the box body 2, which is a circular structure, a square structure, or a polygonal structure, and the boss 7, which is a circular structure, a square structure, or a polygonal structure. Of course, the box body 2 can also be made into any other regular or irregular shape.

[0079] Example 3

[0080] Combination Figure 9 , Figure 10 As shown, this embodiment provides a box for storing biological samples from the inside and outside of a condom. Its basic structure is the same as that of Embodiment 1, but the difference is as follows:

[0081] In this embodiment, the boss 7 is disposed inside the box body 2, and the boss 7 is directly connected to the side wall of the box body 2, and the boss 7 divides the box body 2 into two chambers.

[0082] The boss 7 is a linear pressure structure, such as a straight structure, an arc structure, or a broken line structure.

[0083] A connecting wall 13 is provided on the top of the side wall of the box body 2, and the outer surface of the connecting wall 13 is located inside the side wall; a second groove is provided inside the boss 7; a limiting structure 10 is provided inside the box cover 1, the limiting structure 10 is a linear pressure structure, the linear pressure structure is connected to the side wall of the box cover 1, and a second protrusion is located in the middle of the limiting structure 10. The inner side wall of the box cover 1 is attached to the outer surface of the connecting wall 13, and the second protrusion of the box cover 1 is embedded in the second groove of the boss 7, so that the box body 2 and the box cover 1 are sealed.

[0084] The vertical distance between the inner wall of the bottom of the second groove and the inner wall of the top of the second protrusion is less than the thickness of the sheath 6 of the condom. That is, the sheath portion of the condom located between the second groove and the second protrusion is squeezed and deformed. The inner wall of the bottom of the second groove, the inner wall of the top of the second protrusion, and the sheath portion of the condom between them together form a sealing surface, so that the inner and outer sides of the sheath portion of the condom are isolated.

[0085] In another embodiment, the protrusion 7 is provided with a second groove inside; the limiting structure 10 is provided with a second protrusion inside.

[0086] Unlike Examples 1 and 2, when the box contains condom 4 and is closed, as... Figure 11 As shown, the lid 1 and the body 2 only apply and maintain pressure on the sheath portion of the condom located between the protrusion 7 and the limiting structure 10. The sealing surface is formed by the lid 1, the folded double-layer adhesive film 14 of the condom, and the body 2, and its boundary line is actually a closed annular curve.

[0087] like Figure 12 As shown, similar pressure sealing boundary lines can also be intuitively seen as various linear structures such as arcs and broken lines, but in reality they are all formed by the compression of closed annular curves and have closed ends 15.

[0088] In this embodiment, the box body 2 is square or polygonal, and the box cover 1 is square or polygonal.

[0089] As another embodiment of the box body 2, the box body 2 can also be a circular structure.

[0090] Therefore, in this embodiment, the box can be any form of box body 2 with a circular, square, or polygonal structure, combined with any form of protrusion 7 with a straight, arc, or zigzag structure. Of course, the box body 2 can also be made into any other regular or irregular shape.

[0091] Example 4

[0092] See Figure 13 As shown, the box for storing biological samples from the inside and outside of a condom provided in this embodiment differs from Embodiments 1, 2, or 3 in that:

[0093] In this embodiment, the box also includes a spike 16. At least one spike 16 is provided inside the box cover 1, and the spike 16 is located in the cavity where the sheath 6 of the condom is located. It is used to puncture the liquid biological sample detection reagent wrapped in the membrane placed in the box body 2, and the spike 16 does not contact the sheath 6 of the condom.

[0094] The spike 16 has a tip and a fixed end. The fixed end is disposed on the lid 1. When the lid 1 and the box body 2 are closed, the tip contacts the bottom of the chamber where the sheath 6 of the condom is located.

[0095] The spiked portion 16 is a spike.

[0096] When the cavity containing the sheath 6 of the condom contains liquid cell preservation fluid wrapped in the sac 17, and the condom 4 containing the biological sample is placed into the box 2, when the box lid 1 is fastened and sealed to the box 2, the spikes puncture the sac 17, and the cell preservation fluid flows out and comes into contact with the biological sample in the condom 4, thus playing a role in the active preservation of the cells in the biological sample.

[0097] Example 5

[0098] See Figure 14 As shown, the box for storing biological samples from the inside and outside of a condom provided in this embodiment differs from that in Embodiment 4 in that:

[0099] A button 18 is added to the outside of the box cover 1. The button 18 is connected to the spike 16 inside the box body 2. When the button 18 is operated, the tip of the spike 16 contacts the bottom of the chamber. When the button 18 is not operated, the tip of the spike 16 is in a non-contact state with the bottom of the chamber.

[0100] The button 18 and the spike 16 are rotatably connected. Rotating the button 18 moves the spike 16, allowing the timing of puncturing the liquid cell preservation solution enclosed in the membrane 17. Therefore, in this embodiment, the timing of puncturing the liquid cell preservation solution enclosed in the membrane 14 can be selected, either when the box is closed or after it has been closed.

[0101] Specifically, the button 18 can be a knob.

[0102] The button 18 and the spike 16 are elastically connected. After the button 18 is pressed, the spike 16 moves. In this embodiment, the timing of puncturing the liquid cell preservation solution wrapped in the membrane 17 can be controlled.

[0103] Example 6

[0104] This embodiment provides a biological sample pretreatment method, including the following steps:

[0105] Step S1: Using any one of the boxes from Examples 1 to 5, pre-store the biological sample detection reagent in the cavity of the sheath 6 portion used to store the condom;

[0106] Step S2: Place the condom 4 containing the biological sample into the box 2;

[0107] Step S3: Control the contact between the biological samples on the inside and outside of the condom 4 and the biological sample detection reagent to complete the pretreatment.

[0108] The biological sample testing reagents include preservatives, culture media, lysis agents, staining agents, and precipitants. For different testing needs, the corresponding testing reagents can be pre-placed in the container to achieve the appropriate pretreatment function.

[0109] For example, when the biomarker in the sample is easily inactivated, a preservative can be placed; when the biomarker content in the sample is low and needs to be proliferated, a culture medium can be placed; when the biomarker in the sample is present in the cells, a lysis agent can be placed in advance to lyse the cells; when the biomarker in the sample needs to be stained for detection, a staining agent can be placed in advance for staining; when impurities in the sample will affect the detection, a precipitant can be placed in advance to separate and enrich the sample.

[0110] The biological sample detection reagents can be pre-placed in the aforementioned box in various physical forms. For example, they can be pre-placed in a liquid form encapsulated in a membrane, with the membrane punctured when the box is closed to allow the reagents to flow out; they can also be pre-placed in a solid powder form in a compartment, with the compartment opened when the box is closed to allow the solid powders to come into contact with the sample; alternatively, the detection reagents can be directly coated onto the box wall, with the sample simply contacting the box wall when the box is closed; or the detection reagents can be prepared in the form of a hydrogel, with the hydrogel pre-placed in the box 2, where it can also serve as a pretreatment through osmosis and diffusion after contacting the biological sample.

[0111] The biological detection reagent can automatically come into contact with the biological sample after the lid 1 and the body 2 are closed, or the contact time between the detection reagent and the biological sample can be selected manually. For example, if the detection reagent is made into a temperature-sensitive hydrogel, the contact time between the detection reagent and the biological sample can be manually selected by temperature control.

[0112] If the box of Example 4 is used, the biological detection reagent can automatically come into contact with the biological sample after the box is sealed.

[0113] If the box of Example 5 is used, the contact time between the biological detection reagent and the biological sample can also be selected by manually controlling the button.

[0114] The above specific embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to examples, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A box for storing biological samples from the inside and outside of a condom, comprising a box body and a lid, wherein the lid is capable of sealing the box body, characterized in that, The box contains a storage space for storing condoms, and a sealing structure is provided within the storage space. When a condom is placed inside the box and the lid seals the box, the sheath of the condom is subjected to pressure, isolating the inner side of the condom from the outer side. The sealing structure is a boss that divides the storage space of the box into two chambers. When storing used condoms, the rolled edge of the condom and the sheath portion containing biological samples on the inner and outer sides of the condom are located in different chambers. A limiting structure is provided inside the lid. When the box and the lid are closed, the limiting structure and the boss cooperate to seal the box. The sheath is subjected to pressure deformation from the sealing structure of the box and the limiting structure of the lid, isolating the inner and outer sides of the sheath.

2. The box according to claim 1, characterized in that, The sealing structure is a boss located inside the box and indirectly connected to the side wall of the box; when placing a condom, the rolled edge of the condom is placed between the boss and the side wall of the box.

3. The box according to claim 2, characterized in that, The box body includes a bottom wall, and a side wall is provided on the outer edge of the bottom wall. An extension wall is connected to the upper end of the side wall and extends outward along the horizontal direction of the box body. An extension wall protrusion is provided on the upper surface of the outer edge of the extension wall. The limiting structure is a first protrusion. A retaining ring is provided on the edge of the lower surface of the box cover. The first protrusion is embedded in the protrusion, and the retaining ring is engaged with the outside of the extension wall protrusion to seal the box body and the box cover.

4. The box according to claim 3, characterized in that, The inner edge of the extended wall extends inward along the horizontal direction of the box body. A boss is provided on the upper surface of the inner edge of the extended wall. When the box lid seals the box body, the planar distance between the side wall of the limiting structure and the inner wall of the boss is less than the thickness of the condom film. The condom film portion located between the side wall of the limiting structure and the inner wall of the boss is squeezed and deformed. The side wall of the limiting structure, the inner wall of the boss, and the condom film portion between them form a sealing surface, thereby isolating the inner and outer sides of the condom film portion.

5. The box according to claim 4, characterized in that, When the lid seals the box body, the rolled edge of the condom is placed within the space formed by the boss, the extension wall protrusion, the extension wall, and the lid portion above the extension wall. The vertical distance between the inner wall portion of the lid that contacts the top of the rolled edge of the condom and the outer wall portion of the extension wall that contacts the bottom of the rolled edge of the condom is less than the thickness of the rolled edge of the condom. That is, the rolled edge of the condom is compressed and deformed. The rolled edge of the condom, the inner surface portion of the lid that contacts the top of the rolled edge of the condom, and the outer surface portion of the extension wall that contacts the bottom of the rolled edge of the condom form a sealing surface to prevent liquid from leaking out from the inside of the condom's sheath.

6. The box according to claim 4, characterized in that, The box also includes a locking structure for locking the lid and the box body; the locking structure is a snap fastener located below the snap ring and engaged with the outer edge of the extension wall; or, the locking structure is a latch and a snap fastener, with the latch located on one side of the lid and the snap fastener located on one side of the box body, the latch engaging with the snap fastener to lock the box body and the lid.

7. The box according to claim 1, characterized in that, The sealing structure is a boss located inside the box and directly connected to the side wall of the box.

8. The box according to claim 7, characterized in that, A connecting wall is provided on the top of the side wall of the box body, and the outer surface of the connecting wall is located inside the side wall of the box body. A second groove is provided inside the protrusion. The limiting structure is a linear pressure structure, which is connected to the side wall of the box lid. A second protrusion is provided inside the linear pressure structure. The inner side wall of the box lid is attached to the outer surface of the connecting wall. The second protrusion is embedded in the second groove, so that the box body and the box lid are sealed. The vertical distance between the inner wall of the bottom of the second groove and the inner wall of the top of the second protrusion is less than the thickness of the condom film. That is, the condom film portion located between the second groove and the second protrusion is squeezed and deformed. The inner wall of the bottom of the second groove, the inner wall of the top of the second protrusion, and the condom film portion between them together form a sealing surface, so that the inner and outer sides of the condom film portion are isolated.

9. The box according to any one of claims 1-8, characterized in that, The box lid has at least one spike inside, which is located in the cavity where the sheath of the condom is located. The spike is used to puncture the liquid biological sample detection reagent enclosed in the membrane placed inside the box, and the spike does not come into contact with the sheath of the condom.

10. The box according to claim 9, characterized in that, A button is provided on the outside of the box cover. The button is connected to the spike. When the button is operated, the tip of the spike contacts the bottom of the chamber. When the button is not operated, the tip of the spike is not in contact with the bottom of the chamber.

11. The box according to claim 10, characterized in that, The button and the spike are rotatably connected, and the spike moves by rotating the button; or, the button and the spike are elastically connected, and the spike moves by squeezing the button.

12. A method for preprocessing biological samples, characterized in that, Includes the following steps: Step S1: Using the box described in claim 1, pre-store the biological sample detection reagent in the cavity for storing the sheath portion of the condom; Step S2: Place the condom containing the biological sample into the box; Step S3: Control the contact between the biological samples on the inside and outside of the condom and the biological sample detection reagent to complete the pretreatment.

13. The biological sample pretreatment method according to claim 12, characterized in that, The biological sample testing reagent is stored in solid powder form. The biological sample testing reagent is coated on the inner wall of the box so that the biological sample on the outside of the condom comes into contact with the inner wall of the box to complete the pretreatment.

14. The biological sample pretreatment method according to claim 12, characterized in that, Biological sample testing reagents are liquids encapsulated in membrane sacs. By puncturing the membrane sac, the testing reagents flow out and come into contact with the biological sample to complete the pretreatment.

15. The biological sample pretreatment method according to claim 12, characterized in that, The biological sample testing reagent is in the form of a hydrogel. After the hydrogel comes into contact with the biological sample, it completes the pretreatment through osmosis and diffusion.

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