Application of a bacterial strain fermentation product in preparing a composition for preventing hair loss or promoting hair growth

Abstract

The present invention relates to the technical field of probiotics, and in particular to an application of a strain fermentation product in preparing a composition for preventing hair loss or promoting hair growth, wherein the strain fermentation product is obtained by planting and culturing the Bifidobacterium breve strain FL-01 with a preservation number of CGMCC No.30501 in a soybean milk matrix. The strain fermentation product of the present invention can prevent hair loss or promote hair growth by inhibiting the activity of 5α-reductase, so it can be used to prepare a natural product for preventing hair loss or promoting hair growth to solve the problem of hair loss.

Description

Technical Field

[0001] The invention relates to the technical field of probiotics, and in particular to application of a strain fermentation product in preparing a composition for preventing hair loss or promoting hair growth. Background Art

[0002] Hair loss has always been a problem for most men. There are many reasons for hair loss. About 25% to 50% of male hair loss is caused by male hormones. Figure 1 The male hormone testosterone will bind to 5α-reductase and be converted into dihydrotestosterone (DHT), which then binds to the androgen receptor in the hair, causing hair thinning or hair loss.

[0003] Recent studies have found that isoflavones can inhibit the synthesis of 5α-reductase, and further found that many different plant extracts are helpful to slow down hair loss to a certain extent. Equol is a type of isoflavone. In 2009, Japanese scholars administered equol to prostate cancer patients and found that the testosterone concentration of the patients did not change, but the dihydrotestosterone content in the blood decreased. It can be seen that equol can mainly bind to 5α-reductase, reduce the formation of dihydrotestosterone, and thus avoid the problem of hair loss. Therefore, the development of novel natural 5α-reductase inhibitors is indeed one of the issues that people in the technical field of the present invention are actively solving.

[0004] The patent of Taiwan region of China with the announcement number TWI802367B discloses a probiotic strain for producing equol and its related applications. The probiotic strain includes Bifidobacterium longum strain 536, Lactobacillus paracasei strain PA-88, Bifidobacterium breve strain FL-01, Lactobacillus acidophilus strain AC-69, Bifidobacterium longum subsp. longum strain Gold-01, Bifidobacterium bifidum strain eq-01, and Lactococcus lactis strain CO-17, which can produce equol in soy milk matrix. Based on the above prior art, the present invention explores strains that can produce equol, such as: Bifidobacterium longum subsp. longum ( Bifidobacterium longum ) strain Gold-01, Bifidobacterium breve ( Bifidobacterium breve ) strain FL-01 and anti-inflammatory strains, such as Lactobacillus salivarius ( Ligilactobacillus salivarius ) strain SV-58, after fermenting soy milk, can it enhance the effect of soy milk in inhibiting the activity of 5α-reductase? Based on the characteristic of inhibiting the activity of 5α-reductase, study whether it has the possibility of slowing down hair loss, so as to further apply it to solving the problem of hair loss as a natural product to prevent hair loss or promote hair growth. Summary of the invention

[0005] The purpose of the present application is to address the deficiencies of the prior art and to provide an application of a strain fermentation product in the preparation of a composition for preventing hair loss or promoting hair growth.

[0006] The above invention objectives of the present application are achieved through the following technical solutions:

[0007] Provided is an application of a strain fermentation product in preparing a composition for preventing hair loss or promoting hair growth. The strain fermentation product is obtained by planting and culturing Bifidobacterium breve strain FL-01 (with a preservation number of CGMCC No. 30501) in a soybean milk matrix.

[0008] Preferably, 10 7 CFU~10 9 CFU of the Bifidobacterium breve strain FL-01.

[0009] The present invention also provides an application of a strain fermentation composition in preparing a composition for preventing hair loss or promoting hair growth. The strain fermentation composition comprises a strain fermentation product, which is a strain fermentation product obtained by co-planting and culturing Bifidobacterium longum subsp. longum strain Gold-01 with a preservation number of CGMCC No. 30502, Bifidobacterium breve strain FL-01 with a preservation number of CGMCC No. 30501, and saliva combined with Lactobacillus strain SV-58 with a preservation number of CGMCC No. 30503 in a milk matrix.

[0010] Preferably, the total strain amount of the Bifidobacterium longum subspecies longum Gold-01, the Bifidobacterium breve strain FL-01 and the Lactobacillus salivarius strain SV-58 planted in each 100 mL of the milk matrix is ​​10 7 CFU~10 9 CFU.

[0011] More preferably, the strain amount ratio of the Bifidobacterium longum subsp. longum strain Gold-01, the Bifidobacterium breve strain FL-01 and the Lactobacillus salivarius strain SV-58 is 1:1:1.

[0012] Preferably, the fermented product of the strain is used to prevent hair loss or promote hair growth by inhibiting the activity of 5α-reductase, and the 5α-reductase is type 1 5α-reductase or type 2 5α-reductase.

[0013] Preferably, the culture temperature of the strain fermentation product is 34°C to 40°C, and the culture time is 40 h to 56 h.

[0014] Preferably, the strain fermentation composition comprises the strain fermentation product and Bifidobacterium breve strain FL-01.

[0015] More preferably, the composition for preventing hair loss or promoting hair growth comprises 200 mg to 400 mg of the fermented product of the strain and 10 8 CFU ~ 10 9 CFU of the Bifidobacterium breve strain FL-01.

[0016] Preferably, the composition for preventing hair loss or promoting hair growth is used to improve scalp redness, scalp itching or oily hair.

[0017] The invention provides an application of a strain fermentation product in preparing a composition for preventing hair loss or promoting hair growth. The strain fermentation product is obtained by planting and culturing a Bifidobacterium breve strain FL-01 with a preservation number of CGMCC No.30501 in a soybean milk matrix; and the strain fermentation product is obtained by planting and culturing a Bifidobacterium longum subspecies longum strain Gold-01 with a preservation number of CGMCC No.30502, a Bifidobacterium breve strain FL-01 with a preservation number of CGMCC No.30501 and a salivary combined lactobacillus strain SV-58 with a preservation number of CGMCC No.30503 in a milk matrix. The present invention uses enzyme-bound immunosorbent analysis results after different strain fermentations react with human SRD5α2 to show that the Bifidobacterium breve strain FL-01 can produce components that can inhibit the activity of human SRD5α2 by fermenting soy milk, and milk has more precursors that can be converted into components that inhibit the activity of human SRD5α2 than soy milk, which indicates that the fermentation product of the strain can prevent hair loss or promote hair growth by inhibiting the activity of 5α-reductase; further, the present invention uses experiments on male subjects with hair loss problems but not baldness to prove that the fermentation product of the strain has the effects of preventing hair loss or promoting hair growth and improving scalp redness, itchy scalp or oily hair, so the fermentation product of the strain can be used to prepare natural products for preventing hair loss or promoting hair growth to solve the problem of hair loss. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a diagram of the physiological mechanism of hair loss caused by testosterone being metabolized into dihydrotestosterone (DHT);

[0019] Figure 2 This is a diagram showing the results of enzyme binding immunosorbent analysis of the fermented products of different strains in Example 2 after acting on human SRD5α2;

[0020] Figure 3 This is a comparison of the scalp conditions of the subjects in Example 3 before and after taking the capsules orally;

[0021] Figure 4 This is a comparison of the scalp conditions of the subjects in Example 3 before and after taking the capsules orally;

[0022] Figure 5 This is a comparison of the scalp conditions of the subjects in Example 3 before and after taking the capsules orally;

[0023] Figure 6 This is a comparison of the scalp conditions of the subjects in Example 3 before and after taking the capsules orally;

[0024] Figure 7 This is a comparison of the scalp conditions of the test subject in Example 3 before and after oral administration of the capsule. DETAILED DESCRIPTION

[0025] The present application is further described in detail below in conjunction with the accompanying drawings.

[0026] The microbial strain materials involved in the following examples are as follows:

[0027] Bifidobacterium breve (

[0028] Bifidobacterium breve

[0029] ) strain FL-01, deposited with CGMCC No.30501;

[0030] Bifidobacterium longum subsp. longum (

[0031] Bifidobacterium longum subsp.longum

[0032] ) strain Gold-01, deposited with CGMCC No.30502;

[0033] Lactobacillus salivae (

[0034] Ligilactobacillus salivarius

[0035] ) strain SV-58, deposited with CGMCC No.30503;

[0036] The above microbial strains are all deposited in the General Microbiology Center of China Microbiological Culture Collection Administration, and the deposit date is May 6, 2024.

[0037] According to the patent in Taiwan, China with the publication number TWI802367B, the equol content produced by fermenting soy milk with Bifidobacterium longum subsp. longum strain Gold-01 is higher than that with Bifidobacterium breve strain FL-01. According to the prior art, equol can bind to 5α-reductase and reduce the formation of dihydrotestosterone. Therefore, in theory, the inhibitory effect of soy milk fermented with Bifidobacterium longum subsp. longum strain Gold-01 on the activity of 5α-reductase should be better than that of Bifidobacterium breve strain FL-01, but in fact it is not the case. The above shows that in addition to equol, soy milk fermented with Bifidobacterium breve strain FL-01 also contains other components that can bind to 5α-reductase.

[0038] The first embodiment of the present invention proposes a new use of a strain fermentation product in preparing a composition for preventing hair loss or promoting hair growth, wherein the strain fermentation product is obtained by planting and culturing Bifidobacterium breve strain FL-01 (deposit number: CGMCC No. 30501) in a soy milk matrix. Specifically, 10 strains of Bifidobacterium breve FL-01 (deposit number: CGMCC No. 30501) can be planted in every 100 mL of soy milk matrix. 7 CFU to 10 9 CFU of Bifidobacterium breve strain FL-01, preferably 10 7 CFU, 5×10 7 CFU, 10 8 CFU, 5×10 8 CFU, or 10 9 In addition, the culture temperature is 34°C to 40°C, preferably 34°C, 35°C, 36°C, 37°C, 38°C, 39°C or 40°C; the culture time is 40 hours to 56 hours, preferably 40 hours, 44 hours, 48 ​​hours, 52 hours or 56 hours.

[0039] The strain fermentation product may be a soymilk matrix fermentation liquid containing active bacteria, a soymilk matrix fermentation liquid containing inactivated bacteria, a soymilk matrix fermentation liquid without bacteria, or a powder of any fermentation liquid. Specifically, after culturing, the soymilk matrix containing the strain may be subjected to solid-liquid separation to obtain a liquid portion as a strain fermentation product (i.e., a soymilk matrix fermentation liquid without bacteria), and the solid-liquid separation may be centrifugal separation or gravity sedimentation. In addition, after culturing, the soymilk matrix containing the strain may be subjected to low-temperature drying to obtain a dry powder as a strain fermentation product (i.e., a powder of a soymilk matrix fermentation liquid containing active bacteria, or a powder of a soymilk matrix fermentation liquid containing inactivated bacteria). In addition, after solid-liquid separation, the obtained liquid portion may be subjected to low-temperature drying to obtain a dry powder as a strain fermentation product (i.e., a powder of a soymilk matrix fermentation liquid without bacteria).

[0040] The fermented product of the strain is used to prepare a composition for preventing hair loss or promoting hair growth, and the composition can be orally used for individuals who need to prevent hair loss or promote hair growth. Specifically, the composition can achieve the purpose of preventing hair loss or promoting hair growth by inhibiting the activity of 5α-reductase, wherein the 5α-reductase can be type 1 5α-reductase or type 2 5α-reductase. Type 1 5α-reductase is normally present in the sebaceous glands and liver of the skin, and type 2 5α-reductase is normally present in the prostate, testicles, liver, and hair follicles. Moreover, the composition contains 200 mg to 600 mg of the fermented product of the strain, preferably 200 mg, 300 mg, 400 mg, 500 mg, or 600 mg of the fermented product of the strain.

[0041] The second embodiment of the present invention proposes a new use of a strain fermentation composition in the preparation of a composition for preventing hair loss or promoting hair growth, wherein the strain fermentation composition comprises a strain fermentation product, which is obtained by planting and culturing Bifidobacterium longum subsp. longum strain Gold-01 (preservation number: CGMCC No.30502), Bifidobacterium breve strain FL-01 (preservation number: CGMCC No.30501) and Lactobacillus salivae SV-58 (preservation number: CGMCC No.30503) in a milk matrix. Specifically, a total amount of 10 strains can be planted in each 100 mL of milk matrix. 7 CFU to 10 9 CFU of Bifidobacterium longum subspecies longum Gold-01, Bifidobacterium breve strain FL-01 and Lactobacillus salivarius strain SV-58; preferably, the total strain amount is 10 7 CFU, 5×10 7 CFU, 10 8 CFU, 5×10 8 CFU, or 10 9 CFU. In addition, the planting strain ratio of Bifidobacterium longum subspecies longus strain Gold-01, Bifidobacterium breve strain FL-01 and Lactobacillus salivarius strain SV-58 can be 1: (0.5~1.5): (0.5~1.5); preferably, the planting strain ratio of the above three planting strains is 1: 0.5: 0.5, 1: 1: 0.5, 1: 1.5: 0.5, 1: 0.5: 1, 1: 1: 1, 1: 1.5: 1, 1: 0.5: 1.5, 1: 1: 1.5, or 1: 1.5: 1.5. In addition, the temperature used during cultivation can be 34° C. to 40° C., preferably 34° C., 35° C., 36° C., 37° C., 38° C., 39° C., or 40° C.; the time used during cultivation can be 40 hours to 56 hours, preferably 40 hours, 44 hours, 48 ​​hours, 52 hours, or 56 hours.

[0042] The strain fermentation product may be a milk matrix fermentation liquid containing active bacteria, a milk matrix fermentation liquid containing inactivated bacteria, a milk matrix fermentation liquid without bacteria, or a powder of any fermentation liquid. Specifically, after culturing, the milk matrix containing the strain may be subjected to solid-liquid separation to obtain a liquid portion as a strain fermentation product (i.e., a milk matrix fermentation liquid without bacteria), and the solid-liquid separation may be centrifugal separation or gravity sedimentation. In addition, after culturing, the milk matrix containing the strain may be subjected to low-temperature drying to obtain a dry powder as a strain fermentation product (i.e., a powder of a milk matrix fermentation liquid containing active bacteria, or a powder of a milk matrix fermentation liquid containing inactivated bacteria). In addition, after solid-liquid separation, the obtained liquid portion may be subjected to low-temperature drying to obtain a dry powder as a strain fermentation product (i.e., a powder of a milk matrix fermentation liquid without bacteria).

[0043] The bacterial strain fermentation composition is used to prepare a composition for preventing hair loss or promoting hair growth, and the composition can be orally used for individuals who need to prevent hair loss or promote hair growth. Specifically, the composition can achieve the purpose of preventing hair loss or promoting hair growth by inhibiting the activity of 5α-reductase, wherein the 5α-reductase can be type 1 5α-reductase or type 2 5α-reductase. Type 1 5α-reductase is normally present in the sebaceous glands and liver of the skin, and type 2 5α-reductase is normally present in the prostate, testicles, liver, and hair follicles. In addition, the composition can also achieve the purpose of improving scalp redness, itchy scalp, or oily hair. Moreover, the composition contains 200 mg to 600 mg of the bacterial strain fermentation product, preferably 200 mg, 300 mg, 400 mg, 500 mg, or 600 mg of the bacterial strain fermentation product.

[0044] As a preferred embodiment, the strain fermentation composition includes a strain fermentation product and a Bifidobacterium breve strain FL-01; in order to distinguish it from the "Bifidobacterium breve strain FL-01" used to prepare the strain fermentation product, the Bifidobacterium breve strain FL-01 contained in the strain fermentation composition is hereinafter referred to as the "selectively added Bifidobacterium breve strain FL-01". Under the condition that the strain fermentation composition contains the selectively added Bifidobacterium breve strain FL-01, the prepared composition for preventing hair loss or promoting hair growth contains 200 mg to 600 mg of the strain fermentation product and 10 8 CFU to 10 9 CFU of the selected added Bifidobacterium breve strain FL-01, preferably 200 mg, 300 mg, 400 mg, 500 mg, or 600 mg of the fermentation product of the strain and 10 8 CFU, 2×10 8 CFU, 4×10 8 CFU, 6×10 8 CFU, 8×10 8 CFU, or 10 9 CFU of the selectively added Bifidobacterium breve strain FL-01. In addition, the selectively added Bifidobacterium breve strain FL-01 may be an active strain or a deactivated strain.

[0045] The present application is further described in detail below in conjunction with embodiments and drawings.

[0046] Example 1: Preparation of strain fermentation products

[0047] Obtain high temperature sterilized soy milk from the market. 8CFU Bifidobacterium breve strain FL-01 (preservation number: CGMCC No.30501), Bifidobacterium longum subspecies long strain Gold-01 (preservation number: CGMCC No.30502), Lactobacillus salivae SV-58 (preservation number: CGMCC No.30503), and a mixture of the above three strains (i.e., mixed strains) were added to 100 mL of soy milk and cultured and fermented at 37°C for 48 hours. Finally, after centrifuging the bacterial solution at 3000 rpm, the clarified supernatant was taken as the fermentation product. In addition, a blank group without any strain was used as the reference group, and the commercially available postbiotic product FaciLac was taken as the reference group, wherein the FaciLac product is a product of the above three strains fermented together in skim milk, which is purchased from FacLac Biotech CO., LTD. The fermentations of different strains obtained in this example are shown in Table 1.

[0048] Table 1. Fermentation products of strains

[0049]

[0050] Example 2: 5α-reductase activity test

[0051] The fermentation product of the strain prepared in Example 1 was added to 20 ng / mL human SRD5α2 (steroid 5α reductase 2) and allowed to act at 37° C. for 2 hours. Then, SRD5α2 was quantified using a human SRD5α2 enzyme-linked immunosorbent assay (ELISA) kit purchased from ELKBiotechnology CO., Ltd. (Wuhan).

[0052] The results are as follows Figure 2 As shown in Table 2, the OD of all fermentation products of strains relative to 10 ng / mL human SRD5α2 450 However, compared with the blank group, only the OD of the fermentation product of FL-01 strain decreased. 450 The values ​​of the fermented products of the other two strains decreased, while those of the fermented products of the other two strains increased. It can be inferred that strain FL-01 can produce components that can inhibit the activity of human SRD5α2 through fermentation of soy milk, while strains Gold-01 and strain SV-58 can produce components that protect human SRD5α2 or produce components with similar activity to human SRD5α2 through fermentation of soy milk.

[0053] Please continue to refer to Figure 2 Table 2, OD of mixed strain fermentation products 450The value was lower than that of the blank group, but higher than that of the FL-01 fermentation. After comparing the mixed strain fermentation with FaciLac (reference group), it was found that the OD 450 The value is lower than that of the mixed strain fermentation product, which can be inferred that cow's milk has more precursors that can be converted into components that inhibit the activity of human SRD5α2 than soy milk, while soy milk has more precursors that can be converted into components that protect human SRD5α2 or produce or convert into components with activity similar to human SRD5α2 than cow's milk.

[0054] Table 2. 5α-reductase activity

[0055]

[0056] From this, it can be seen that for the FL-01 strain fermentation product, the strain FL-01 can produce components that can inhibit the activity of human SRD5α2 by fermenting soy milk; for the strain fermentation composition (mixed strain fermentation product), milk has more precursors that can be converted into components that inhibit the activity of human SRD5α2 than soy milk.

[0057] Example 3: Human testing

[0058] According to the experimental results of Example 2, a mixture of FaciLac powder and Bifidobacterium breve FL-01 of Example 1 was selected as the strain fermentation composition, and soft-shell or hard-shell capsules were prepared by conventional methods, each capsule containing 150 mg of FaciLac powder and 10 8 CFU of Bifidobacterium breve strain FL-01, two capsules per person per day.

[0059] 16 men aged about 40 who had hair loss but not baldness were selected as subjects. Among them, 6 subjects also had scalp redness and swelling, and 8 subjects also had scalp itching. The test period was 12 weeks (about 3 months), and the scalp condition was photographed before and after the test and confirmed with a questionnaire.

[0060] The comparison results of the scalp conditions of the above subjects before and after taking the capsules are as follows:

[0061] like Figure 3 As shown, about 14 of the 16 subjects (88%) had a significant increase in hair density after taking the capsules. In addition to hair loss, about 6 subjects also had scalp redness and swelling, of which 5 (83%) said that the scalp redness and swelling problem was improved after taking the capsules. In addition to hair loss, about 8 subjects also had scalp itching problems, of which 6 (75%) said that the scalp itching problem was improved after taking the capsules.

[0062] like Figure 4 and Figure 5As shown, the hair density at the mid-parting of the subjects increased significantly after taking the capsules.

[0063] like Figure 6 As shown, after taking the capsules, the density of thinning hair increased significantly and the glossiness of the hair was reduced.

[0064] like Figure 7 As shown, after taking the capsules, the hair density in the sparse areas of the subjects increased significantly, and the redness and swelling of the scalp also improved.

[0065] It can be seen that the fermented product of the strain prepared by the present invention has the effects of promoting hair growth and improving scalp redness, scalp itching or oily hair, and can be used to prepare a composition for preventing hair loss or promoting hair growth.

[0066] The embodiments described above are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. Such modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application, and should all be included in the protection scope of the present application.

Claims

1. Use of a fermented product of a strain in the preparation of a composition for preventing hair loss or promoting hair growth, characterized in that: The strain fermentation product is obtained by planting and culturing the Bifidobacterium breve strain FL-01 with a preservation number of CGMCC No. 30501 in a soybean milk matrix.

2. The use according to claim 1, characterized in that: 10 7 CFU~10 9 CFU of the Bifidobacterium breve strain FL-01.

3. Use of a bacterial strain fermentation composition in the preparation of a composition for preventing hair loss or promoting hair growth, characterized in that: The strain fermentation composition comprises a strain fermentation product, wherein the strain fermentation product is obtained by planting and culturing Bifidobacterium longum subspecies longum Gold-01 with a preservation number of CGMCC No. 30502, Bifidobacterium breve FL-01 with a preservation number of CGMCC No. 30501, and saliva combined with Lactobacillus strain SV-58 with a preservation number of CGMCC No. 30503 in a milk matrix; The strain quantity ratio of the Bifidobacterium longum subspecies longum strain Gold-01, the Bifidobacterium breve strain FL-01 and the Lactobacillus salivarius strain SV-58 is 1:1:

1.

4. The use according to claim 3, characterized in that: The total strain quantity of the Bifidobacterium longum subspecies longum Gold-01, the Bifidobacterium breve strain FL-01 and the Lactobacillus salivarius strain SV-58 planted in each 100 mL of the milk matrix is ​​10 7 CFU~10 9 CFU.

5. The use according to claim 1 or 3, characterized in that: The fermented product of the strain is used to prevent hair loss or promote hair growth by inhibiting the activity of 5α-reductase, and the 5α-reductase is type 1 5α-reductase or type 2 5α-reductase.

6. The use according to claim 1 or 3, characterized in that: The culture temperature of the strain fermentation product is 34°C to 40°C, and the culture time is 40 h to 56 h.

7. The use according to claim 3, characterized in that: The strain fermentation composition comprises the strain fermentation product and Bifidobacterium breve strain FL-01.

8. The use according to claim 7, characterized in that: The composition for preventing hair loss or promoting hair growth comprises 200 mg to 400 mg of the fermented product of the strain and 10 8 CFU ~ 10 9 CFU of the Bifidobacterium breve strain FL-01.

9. The use according to claim 1 or 3, characterized in that: The composition for preventing hair loss or promoting hair growth is used for improving scalp redness, scalp itching or oily hair.

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