A vibrating mixing device and a mixing method for cell culture treatment

Abstract

The present invention discloses an oscillating mixing device and a mixing method for cell culture treatment, which relates to the field of mixing devices, including a vortex oscillation component, a vertical oscillation component and a monomer swing component. The vortex oscillation component includes an output shaft, an oscillation shaft and an oscillation frame. When the output shaft rotates, it can drive the oscillation shaft to move in a circle. The oscillation shaft can drive the oscillation frame to move. The oscillation frame is provided with a plurality of storage holes. The monomer swing component is arranged in the storage hole. The monomer swing component includes a swing sleeve and a swing rod. The swing sleeve is arranged in the storage hole. The rotation direction of the liquid in the container is continuously changed, so that the movement and mutual collision of the liquid in the container are more intense, and the uniformity of the mixing of the liquid material is further improved, so that the clamping plate can clamp containers of different heights, and the adaptability to the container is stronger. The operation of moving the clamping plate and locking it is simpler, so as to prevent the liquid in the container from spilling out, ensure the cleanliness of the equipment, and prevent material waste.

Description

Technical Field

[0001] The invention relates to the field of mixing devices, and in particular to an oscillating mixing device and a mixing method for cell culture treatment. Background Art

[0002] Cell culture is a technique for culturing cells in vitro under controlled conditions. During cell culture, shaking and mixing are required. Shaking can increase the contact area between the culture medium and the air and promote oxygen dissolution, which is especially important for the growth of aerobic cells. Shaking helps maintain the uniformity of the culture medium's temperature, pH value and other culture conditions.

[0003] Chinese patent application CN109985556A discloses a vertical oscillating vortex device, comprising a rectangular box, a motor A, a motor B, a sample tray, an oscillation adjustment knob, a vortex switch, a time regulator, a time display screen, a sample cover, a lifting slide rail, and a fixing device. The motor A is located in the rectangular box, the motor B is located in the sample tray, the oscillation adjustment knob, the vortex switch, the time regulator, and the time display screen are located on one side of the rectangular box, and the sample cover and the sample tray are connected to the lifting slide rail through the fixing device. The device has a simple structure and is easy to use. It combines the functions of the vortex and the vertical oscillator, and can use a certain function alone or simultaneously, thereby realizing multi-purpose use of one machine and saving laboratory space.

[0004] Chinese patent application CN114480124A discloses a multi-dimensional oscillation device for hematopoietic stem cells, comprising a support column, a box body and a flip cover, wherein the box body is divided into a culture chamber in the middle and transmission chambers on both sides by a partition, wherein: a slide groove is respectively provided in the middle of the top of the left and right partitions, and a slider is arranged in the slide groove; the top of the slider abuts against the top of the slide groove through an upper vibration spring, and the bottom end abuts against the bottom of the slide groove through a lower vibration spring, and a rotating shaft is rotatably arranged in the inner hole of the slider; an eccentric wheel is movably fixed to the box body through an eccentric shaft in the transmission chamber, and the edge of the eccentric wheel is connected to the rotating shaft through a hinged rocking rod; the eccentric shaft outside the eccentric wheel is transmission-connected to a driving motor arranged at the bottom of the box body, the slider is used to realize the up and down vibration of the test tube carrier plate, and the rotating shaft is used to realize the left and right swing vibration of the test tube carrier plate with the eccentric wheel, so as to realize the multi-dimensional vibration of the cell culture tube on the test tube carrier plate; and the structural design is simple and novel, which saves time and effort and improves work efficiency.

[0005] The above patents and prior art also have the following defects:

[0006] In the prior art, the vortex oscillator only oscillates the liquid in the culture container in one direction. Although some vortex oscillators have added vertical oscillation, the oscillation in the horizontal direction is single, and the liquid exchange in the culture container is not intense, resulting in poor mixing effect and slow mixing speed. At the same time, some vortex oscillators have added vertical oscillation, which fails to press the top of the culture container tightly, causing the end cover on the top of the culture container to fall off under the oscillation, which easily causes the material in the culture container to spill, polluting the environment and causing material waste.

[0007] Therefore, the present application provides an oscillating mixing device and a mixing method for cell culture processing to meet the needs. Summary of the invention

[0008] The purpose of the present application is to provide an oscillating mixing device and a mixing method for cell culture treatment, which continuously changes the rotation direction of the liquid in the container, makes the liquid move and collide with each other more violently in the container, further improves the uniformity of liquid material mixing, enables the clamping plate to clamp containers of different heights, has stronger adaptability to containers, and makes the operation of moving the clamping plate and locking it simpler, thereby preventing the liquid in the container from spilling, ensuring the cleanliness of the equipment, and preventing material waste.

[0009] To achieve the above-mentioned purpose, the present application provides the following technical solutions: an oscillating mixing device, comprising a vortex oscillation component, a vertical oscillation component and a monomer swing component, wherein the vortex oscillation component comprises an output shaft, an oscillation shaft and an oscillation frame, wherein the output shaft can drive the oscillation shaft to move in a circle when rotating, and the oscillation shaft can drive the oscillation frame to move;

[0010] The oscillation frame is provided with a plurality of storage holes, the single body swing assembly is arranged in the storage holes, the single body swing assembly comprises a swing sleeve and a swing rod, the swing sleeve is arranged in the storage hole, the swing rod is rotatably connected to the outer wall of the swing sleeve, a swing groove is provided in the storage hole, the swing groove is inclined, and the swing rod is slidably fitted in the swing groove;

[0011] When the oscillation shaft moves in a circular motion, the vertical oscillation assembly can cause the swing sleeve to move vertically periodically;

[0012] The vertical oscillation assembly is provided with a top cover clamping assembly, and the top cover clamping assembly includes a fixing rod, a locking plate, a clamping plate, a plurality of fixing blocks, a locking cylinder and a clamping cylinder. The locking cylinder is fixedly mounted on the clamping plate. Limiting grooves are provided on both sides of the fixing rod. A plurality of fixing blocks are fixedly mounted in the limiting grooves at equal distances. The clamping cylinder is threadedly connected in the locking cylinder. When the clamping cylinder moves into the locking cylinder, the locking plate can be pushed between two adjacent fixing blocks.

[0013] When the swing sleeve moves vertically, it can drive the fixing rod to move vertically.

[0014] Preferably, the top cover clamping assembly also includes a limit block, a rotating ring, a push plate and a rotating block, the limit block is fixedly mounted on the inner wall of the locking cylinder, the limit block slides in the limit groove, one end of the locking plate is rotatably connected to the limit block, the rotating ring is fixedly mounted on the inner wall of the locking cylinder, one end of the push plate is rotatably connected to the rotating ring, the other end of the push plate is rotatably connected to the locking plate, the clamping plate is provided with a clamping groove corresponding to the storage hole, the rotating block is fixedly mounted on the outer wall of the locking cylinder, and the locking cylinder is sleeved on the fixed rod.

[0015] Preferably, the oscillation shaft and the output shaft are eccentrically arranged, and the vortex oscillation assembly also includes an output disk, an oscillation disk, an adjustment stud and an adjustment nut. The output shaft is fixedly installed at the center of the output disk, the oscillation shaft is fixedly installed at the center of the oscillation disk, the adjustment stud is fixedly installed on the output disk, an adjustment bar hole is opened on the oscillation disk, the oscillation shaft passes through the adjustment bar hole, the adjustment nut is sleeved on the adjustment stud and threadedly connected with the adjustment stud, and the adjustment nut is against the oscillation disk.

[0016] Preferably, the vortex oscillation assembly also includes a plurality of limit teeth and a clamping block, a connecting groove is opened on the output disk, a plurality of the limit teeth are fixedly installed in the connecting groove, the clamping block is fixedly installed at the bottom of the oscillation disk and slidably fits in the connecting groove, and the clamping block cooperates with the limit teeth.

[0017] Preferably, the oscillation frame is fixedly mounted on the oscillation shell, a fixed shell is arranged under the oscillation shell, the vertical oscillation assembly comprises a moving cylinder, an oscillation spring, a moving block, a moving ring, a moving rod and an oscillation plate, the oscillation shaft is arranged in a rectangular shape, the moving cylinder is sleeved on the oscillation shaft and slidably cooperates with the oscillation shaft, the moving block is fixedly mounted on the moving cylinder, the moving rod is rotatably connected to the moving block, the moving ring is fixedly mounted on the fixed shell, and release grooves are equidistantly provided on the moving ring, one end of the oscillation spring is fixedly mounted on the moving block, and the other end of the oscillation spring is fixedly mounted on the oscillation shell, the oscillation spring is sleeved on the moving cylinder, the moving cylinder passes through the oscillation shell and slidably cooperates with the oscillation shell, the top of the moving cylinder is fixedly mounted on the oscillation plate, and the swing sleeve is rotatably mounted on the oscillation plate.

[0018] Preferably, stabilizing components are provided at the four corners of the bottom of the oscillation shell, and the stabilizing components include a rotating shaft, a sliding guide rail, a sliding block and a stabilizing shaft. The rotating shaft is rotatably connected to the fixed shell, the sliding guide rail is fixedly installed on the rotating shaft, the sliding block is slidably fitted on the sliding guide rail, the stabilizing shaft is fixedly installed on the sliding block, and the top of the stabilizing shaft is rotatably connected to the bottom of the oscillation shell.

[0019] Preferably, a driving assembly is provided in the fixed shell, and the driving assembly can drive the output shaft to rotate. The driving assembly includes a driving motor, a driving shaft and a driving belt member. The driving motor is fixedly installed in the fixed shell, and the driving shaft is fixedly installed at the output end of the driving motor. The driving belt member includes a driving active pulley, a driving passive pulley and a driving belt. The driving active pulley is fixedly installed on the driving shaft, and the driving passive pulley is fixedly installed on the output shaft, and the driving belt is connected to the driving active pulley and the driving passive pulley.

[0020] Preferably, a sliding sleeve is fixedly installed on the top exterior of the swing sleeve, and the sliding sleeve is slidably fitted in the storage hole. A plurality of clamping blocks are fixedly installed in the swing sleeve, and the clamping blocks are made of silicone.

[0021] Preferably, the fixing rod passes through the oscillation frame and is slidably matched with the oscillation frame, the bottom of the fixing rod is fixedly mounted on the oscillation plate, and the oscillation plate is slidably matched in the oscillation housing.

[0022] A mixing method for cell culture treatment, using the above-mentioned oscillating mixing device, comprises the following steps:

[0023] adding cell culture medium to the container, adding a specified amount of cells to the container;

[0024] Place the container in the swing sleeve, press the pressing plate against the top of the container, rotate the locking cylinder, the locking cylinder moves toward the pressing cylinder, and at the same time the locking cylinder pushes the locking plate to move between the two fixed blocks, locking the pressing plate against the top of the container;

[0025] The output shaft drives the oscillation shaft to move in a circle, the oscillation shaft rotates eccentrically relative to the output shaft, the oscillation shaft drives the oscillation frame to move, the oscillation frame drives the swing sleeve to move, the swing sleeve drives the container to move in a circle, the liquid in the container rotates in one direction to form a vortex, and the cells and cell culture medium in the liquid are mixed;

[0026] When the oscillation shaft moves in a circular motion, the vertical oscillation component drives the swing sleeve to move vertically periodically, and the swing sleeve drives the container to move vertically periodically, so that the liquid in the container is mutually exchanged in the vertical direction, and the cells and cell culture medium in the liquid are further mixed;

[0027] When the swing sleeve moves vertically periodically, it drives the swing rod to move vertically periodically. When the swing rod moves upward in the swing groove, the inclined swing groove causes the swing rod to rotate, and the swing rod drives the swing sleeve to rotate. When the swing rod moves downward in the swing groove, the swing rod rotates in the opposite direction, so that the swing sleeve continuously swings with the periodic vertical movement, so that the liquid in the container continuously changes its rotation direction, thereby being mixed more evenly.

[0028] In summary, the technical effects and advantages of the present invention are as follows:

[0029] 1. In the present invention, the vortex oscillation component is used to rotate the liquid horizontally, so that the liquid forms a vortex, thereby being mixed. The vertical oscillation component is used to oscillate the liquid vertically, so that the liquid is oscillated in multiple directions, thereby being mixed more evenly and having a better mixing effect. When the container moves vertically periodically, the swing sleeve is oscillated periodically, driving the container to move in the opposite direction periodically, constantly changing the rotation direction of the liquid in the container, making the liquid move and collide with each other more violently in the container, and further improving the mixing uniformity of the liquid material;

[0030] 2. In the present invention, when the liquid in the container is shaken and mixed, the pressing plate presses the top of the container to prevent the end cover of the container from detaching under the shaking, prevent the liquid in the container from spilling, ensure the cleanliness of the equipment, and prevent material waste. By moving the pressing plate to the top of the container on the fixed rod and then locking the pressing plate, the position of the pressing plate can be quickly moved and locked, so that the pressing plate can press containers of different heights, the adaptability to the container is stronger, and the operation of moving the pressing plate and locking it is simpler;

[0031] 3. The eccentricity of the oscillation shaft relative to the output shaft can be adjusted, thereby adjusting the oscillation amplitude of the liquid in the container, which is more adaptable;

[0032] 4. While making the oscillation shell move in a circle, the oscillation shell is made more stable, not easy to tilt and shake, with a certain bearing capacity, and prevents the oscillation shell from rotating significantly. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative work.

[0034] Figure 1 It is a schematic diagram of the structure of the fixed housing, the oscillating housing, the top cover pressing assembly and the stabilizing assembly in the present invention;

[0035] Figure 2It is a schematic diagram of the structure of the fixed housing, the oscillating housing and the driving assembly in the present invention;

[0036] Figure 3 It is a schematic diagram of the structure of the oscillation housing, the vertical oscillation assembly and the container in the present invention;

[0037] Figure 4 It is a schematic diagram of the structure of the oscillation frame, the container and the oscillating sleeve in the present invention;

[0038] Figure 5 For the present invention Figure 4 A magnified view of part A;

[0039] Figure 6 For the present invention Figure 4 A magnified view of part B;

[0040] Figure 7 It is a structural schematic diagram of the oscillation frame, container and top cover pressing assembly in the present invention;

[0041] Figure 8 For the present invention Figure 7 Enlarged view of part C;

[0042] Fig. 9 It is a structural schematic diagram of the swing sleeve, the swing rod and the pressing plate in the present invention;

[0043] Fig.10 It is a schematic diagram of the structure of the oscillation frame, the single body oscillating assembly, the oscillation plate and the pressing plate in the present invention;

[0044] Fig.11 For the present invention Fig.10 A magnified view of part D in the middle;

[0045] Fig.12 It is a schematic diagram of the structure of the swing sleeve, the clamping block and the container in the present invention;

[0046] Fig.13 It is a structural schematic diagram of the pressing plate and the fixing rod of the present invention;

[0047] Fig.14 For the present invention Fig.13 Enlarged view of part E.

[0048] In the figure: 1. vortex oscillation assembly; 11. output shaft; 12. oscillation shaft; 13. oscillation frame; 14. output plate; 15. oscillation plate; 16. adjustment stud; 17. adjustment nut; 18. limit tooth; 19. clamping block; 2. vertical oscillation assembly; 21. moving cylinder; 22. oscillation spring; 23. moving block; 24. moving ring; 25. moving rod; 26. oscillation plate; 3. single swing assembly; 31. swing sleeve; 32. swing rod; 4. swing slot; 5. top cover Clamping assembly; 51. fixing rod; 52. locking plate; 53. clamping plate; 54. fixing block; 55. locking cylinder; 56. clamping cylinder; 57. limiting block; 58. pushing plate; 59. rotating block; 6. fixed shell; 7. stabilizing assembly; 71. rotating shaft; 72. sliding guide rail; 73. sliding block; 74. stabilizing shaft; 8. driving assembly; 81. driving motor; 82. driving belt; 9. oscillating shell; 10. clamping block; 20. sliding sleeve; 30. container. DETAILED DESCRIPTION

[0049] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0050] Example 1: Reference Figure 1-Figure 14 The oscillating mixing device shown includes a vortex oscillation component 1, a vertical oscillation component 2 and a monomer swing component 3. The vortex oscillation component 1 includes an output shaft 11, an oscillation shaft 12 and an oscillation frame 13. When the output shaft 11 rotates, it can drive the oscillation shaft 12 to move in a circle, and the oscillation shaft 12 can drive the oscillation frame 13 to move.

[0051] A plurality of storage holes are provided on the oscillation frame 13, and the single swing assembly 3 is arranged in the storage hole. The single swing assembly 3 includes a swing sleeve 31 and a swing rod 32. The swing sleeve 31 is arranged in the storage hole, and the swing rod 32 is rotatably connected to the outer wall of the swing sleeve 31. A swing groove 4 is provided in the storage hole, and the swing groove 4 is inclined, and the swing rod 32 is slidably fitted in the swing groove 4.

[0052] When the oscillation shaft 12 moves in a circular motion, the vertical oscillation assembly 2 can cause the swing sleeve 31 to move vertically periodically;

[0053] A top cover clamping assembly 5 is provided on the vertical oscillation assembly 2. The top cover clamping assembly 5 includes a fixing rod 51, a locking plate 52, a clamping plate 53, a plurality of fixing blocks 54, a locking cylinder 55 and a clamping cylinder 56. The locking cylinder 55 is fixedly installed on the clamping plate 53. Limiting grooves are provided on both sides of the fixing rod 51. A plurality of fixing blocks 54 are fixedly installed in the limiting grooves at equal distances. The clamping cylinder 56 is threadedly connected in the locking cylinder 55. When the clamping cylinder 56 moves into the locking cylinder 55, the locking plate 52 can be pushed between two adjacent fixing blocks 54.

[0054] When the swing sleeve 31 moves vertically, it can drive the fixing rod 51 to move vertically.

[0055] Add liquid material into the container 30; place the container 30 in the swing sleeve 31, press the top of the container 30 with the pressing plate 53, rotate the locking cylinder 55, and the locking cylinder 55 moves toward the pressing cylinder 56. At the same time, the locking cylinder 55 pushes the locking plate 52 to move between the two fixed blocks 54, and locks the pressing plate 53 on the top of the container 30; the output shaft 11 drives the oscillation shaft 12 to move in a circle, and the oscillation shaft 12 rotates eccentrically relative to the output shaft 11. The oscillation shaft 12 drives the oscillation frame 13 to move, and the oscillation frame 13 drives the swing sleeve 31 to move. The swing sleeve 31 drives the container 30 to move in a circle, and the liquid in the container 30 rotates in one direction to form a vortex, and the material in the liquid is mixed; the oscillation shaft 12 rotates in a circle When moving, the vertical oscillation component 2 drives the swing sleeve 31 to move vertically periodically, and the swing sleeve 31 drives the container 30 to move vertically periodically. The liquid in the container 30 is exchanged with each other in the vertical direction, and the material in the liquid is further mixed; when the swing sleeve 31 moves vertically periodically, it drives the swing rod 32 to move vertically periodically. When the swing rod 32 moves upward in the swing groove 4, the inclined swing groove 4 causes the swing rod 32 to rotate, and the swing rod 32 drives the swing sleeve 31 to rotate. When the swing rod 32 moves downward in the swing groove 4, the swing rod 32 rotates in the opposite direction, so that the swing sleeve 31 continuously swings with the periodic vertical movement, so that the liquid in the container 30 continuously changes the rotation direction, thereby being mixed more evenly.

[0056] The vortex oscillation component 1 is used to rotate the liquid horizontally, so that the liquid forms a vortex and is mixed. The vertical oscillation component 2 is used to oscillate the liquid vertically, so that the liquid is oscillated in multiple directions, so that it is mixed more evenly and has a better mixing effect. When the container 30 moves vertically and periodically, the swing sleeve 31 is oscillated periodically to drive the container 30 to move in the opposite direction periodically, and the rotation direction of the liquid in the container 30 is constantly changed, so that the movement and mutual collision of the liquid in the container 30 are more intense, and the uniformity of liquid material mixing is further improved. When the liquid in the container 30 is oscillated and mixed, the clamping plate 53 presses the top of the container 30 to prevent the end cover of the container 30 from detaching under the oscillation, and prevents the liquid in the container 30 from spilling out, so as to ensure the cleanliness of the equipment and prevent material waste. By moving the clamping plate 53 to the top of the container 30 on the fixing rod 51 and then locking the clamping plate 53, the position of the clamping plate 53 can be quickly moved and locked, so that the clamping plate 53 can clamp containers 30 of different heights, and the adaptability to the container 30 is stronger, and the operation of moving and locking the clamping plate 53 is simpler.

[0057] A rubber pad is fixedly installed at the bottom of the clamping plate 53, which has a better clamping effect on the top of the container 30 and prevents the top of the container 30 from being worn. After the liquid in the container 30 is mixed, the locking cylinder 55 is rotated to move the locking cylinder 55 outward of the clamping cylinder 56, and the clamping cylinder 56 drives the locking plate 52 to rotate, and the end of the locking plate 52 moves away from the corresponding two fixed blocks 54. The bottom of the locking plate 52 can drive the clamping plate 53 and the clamping cylinder 56 to move downward to squeeze the rubber pad, so that the top of the locking plate 52 can be smoothly separated from between the two fixed blocks 54.

[0058] Example 2, refer to Figure 1-Figure 14 , which is different from the above-mentioned embodiment, the top cover clamping assembly 5 also includes a limit block 57, a rotating ring, a push plate 58 and a rotating block 59. The limit block 57 is fixedly mounted on the inner wall of the locking cylinder 55, and the limit block 57 is slidably fitted in the limit groove. One end of the locking plate 52 is rotatably connected to the limit block 57, the rotating ring is fixedly mounted on the inner wall of the locking cylinder 55, one end of the push plate 58 is rotatably connected to the rotating ring, and the other end of the push plate 58 is rotatably connected to the locking plate 52. A clamping groove is provided on the clamping plate 53 corresponding to the storage hole. The rotating block 59 is fixedly mounted on the outer wall of the locking cylinder 55, and the locking cylinder 55 is sleeved on the fixed rod 51.

[0059] When the locking cylinder 55 rotates and moves toward the clamping cylinder 56, the locking cylinder 55 vertically drives the rotating ring to move vertically, the rotating ring drives the top of the pushing plate 58 to move vertically, and the bottom of the pushing plate 58 drives the locking plate 52 to rotate on the limit block 57. The end of the locking plate 52 moves between the two fixed blocks 54. The locking plate 52 is located between the two fixed blocks 54 and cannot rise, so that the clamping plate 53 cannot rise, thereby limiting the container 30.

[0060] Example 3, refer to Figure 1-Figure 14 , which is different from the above-mentioned embodiment, the oscillation shaft 12 is eccentrically arranged with the output shaft 11, the vortex oscillation assembly 1 also includes an output disk 14, an oscillation disk 15, an adjusting stud 16 and an adjusting nut 17, the output shaft 11 is fixedly mounted at the center of the output disk 14, the oscillation shaft 12 is fixedly mounted at the center of the oscillation disk 15, the adjusting stud 16 is fixedly mounted on the output disk 14, an adjusting bar hole is opened on the oscillation disk 15, the oscillation shaft 12 passes through the adjusting bar hole, the adjusting nut 17 is sleeved on the adjusting stud 16 and is threadedly connected with the adjusting stud 16, and the adjusting nut 17 is against the oscillation disk 15.

[0061] When the output shaft 11 rotates, the output disk 14 is driven to rotate, and the output disk 14 drives the adjusting stud 16 to move in a circle, and the adjusting stud 16 drives the adjusting nut 17 to move in a circle, and the adjusting nut 17 drives the oscillation disk 15 to move in a circle, and the oscillation disk 15 drives the oscillation shaft 12 to move in a circle, and the oscillation shaft 12 rotates eccentrically relative to the output shaft 11. When the eccentric distance of the oscillation shaft 12 needs to be adjusted, the adjusting nut 17 is rotated, and the adjusting nut 17 no longer presses the oscillation disk 15, and the oscillation disk 15 is moved to adjust the distance between the oscillation shaft 12 and the output shaft 11. After adjustment, the adjusting nut 17 is rotated to lock the adjusting nut 17, and the adjusting nut 17 locks the oscillation disk 15, so that the eccentricity of the oscillation shaft 12 relative to the output shaft 11 can be adjusted, thereby adjusting the oscillation amplitude of the liquid in the container 30, and the adaptability is stronger.

[0062] Embodiment 4, referring to Figure 1-Figure 14 , which is different from the above-mentioned embodiment, the vortex oscillation assembly 1 also includes a plurality of limiting teeth 18 and a clamping block 19, a connecting groove is opened on the output disk 14, a plurality of limiting teeth 18 are fixedly installed in the connecting groove, the clamping block 19 is fixedly installed at the bottom of the oscillation disk 15 and slidably fits in the connecting groove, and the clamping block 19 cooperates with the limiting teeth 18.

[0063] When the adjusting nut 17 no longer locks the oscillation disk 15, the oscillation disk 15 is moved upward, and the blocking block 19 disengages from the limiting tooth 18, so that the oscillation disk 15 can be moved to adjust the position. Then the adjusting nut 17 locks the oscillation disk 15, and the oscillation disk 15 drives the blocking block 19 to descend, and the blocking block 19 engages with the limiting tooth 18, making the oscillation disk 15 more stable and not easy to shake.

[0064] Example 5, refer to Figure 1-Figure 14, which is different from the above-mentioned embodiment, the oscillation frame 13 is fixedly mounted on the oscillation shell 9, and a fixed shell 6 is arranged below the oscillation shell 9. The vertical oscillation component 2 includes a moving cylinder 21, an oscillation spring 22, a moving block 23, a moving ring 24, a moving rod 25 and an oscillation plate 26. The oscillation shaft 12 is rectangular, and the inner wall of the moving cylinder 21 is rectangular. The moving cylinder 21 is sleeved on the oscillation shaft 12 and slides with the oscillation shaft 12. The moving block 23 is fixedly mounted on the moving cylinder 21, and the moving rod 25 is rotatably connected to the moving block 23. The moving ring 24 is fixedly mounted on the fixed shell 6, and release grooves are equidistantly provided on the moving ring 24. One end of the oscillation spring 22 is fixedly mounted on the moving block 23, and the other end of the oscillation spring 22 is fixedly mounted on the oscillation shell 9. The oscillation spring 22 is sleeved on the moving cylinder 21, and the moving cylinder 21 passes through the oscillation shell 9 and slides with the oscillation shell 9. The top of the moving cylinder 21 is fixedly mounted on the oscillation plate 26, and the swing sleeve 31 is rotatably mounted on the oscillation plate 26.

[0065] The oscillation shaft 12 drives the moving cylinder 21 to move in a circle, and the moving cylinder 21 drives the oscillation shell 9 to move in a circle, and the moving cylinder 21 rotates on the oscillation shell 9, so that the oscillation shell 9 does not rotate by itself, and the oscillation shell 9 drives the oscillation frame 13 to move in a circle, and the oscillation frame 13 oscillates the liquid in the container 30, and at the same time, the moving cylinder 21 drives the moving block 23 to move, and the moving block 23 drives the moving rod 25 to move, and the moving rod 25 moves on the moving ring 24, and the moving ring 24 is also provided with a plurality of release grooves, so that the top of the moving ring 24 forms a wave-shaped structure, and the moving ring 24 periodically squeezes the moving cylinder 21, so that the moving cylinder 21 periodically moves vertically, and the moving cylinder 21 drives the oscillation plate 26 to move vertically periodically, and the oscillation plate 26 drives the swing sleeve 31 to move vertically periodically, and the swing sleeve 31 drives the container 30 to move vertically periodically, so that the liquid and material in the container 30 are vertically oscillated, and the liquid and material are mixed.

[0066] Example 6, refer to Figure 1-Figure 14 , which is different from the above embodiment, a stabilizing component 7 is provided at the four corners of the bottom of the oscillation shell 9, and the stabilizing component 7 includes a rotating shaft 71, a sliding guide rail 72, a sliding block 73 and a stabilizing shaft 74. The rotating shaft 71 is rotatably connected to the fixed shell 6, the sliding guide rail 72 is fixedly installed on the rotating shaft 71, the sliding block 73 is slidably fitted on the sliding guide rail 72, the stabilizing shaft 74 is fixedly installed on the sliding block 73, and the top of the stabilizing shaft 74 is rotatably connected to the bottom of the oscillation shell 9.

[0067] When the moving cylinder 21 drives the oscillation shell 9 to move in a circle, the oscillation shell 9 drives the stabilizing shaft 74 to move, the stabilizing shaft 74 drives the sliding block 73 to move, the sliding block 73 moves on the sliding guide rail 72, and drives the sliding guide rail 72 to rotate, and the sliding guide rail 72 rotates with the rotating shaft 71, so that the oscillation shell 9 moves in a circle and makes the oscillation shell 9 more stable, making it not easy to tilt and shake, having a certain load-bearing capacity, and preventing the oscillation shell 9 from rotating significantly.

[0068] Embodiment 7, reference Figure 1-Figure 14 , which is different from the above-mentioned embodiment, a driving assembly 8 is arranged in the fixed housing 6, and the driving assembly 8 can drive the output shaft 11 to rotate. The driving assembly 8 includes a driving motor 81, a driving shaft and a driving belt member 82. The driving motor 81 is fixedly installed in the fixed housing 6, and the driving shaft is fixedly installed at the output end of the driving motor 81. The driving belt member 82 includes a driving active pulley, a driving passive pulley and a driving belt. The driving active pulley is fixedly installed on the driving shaft, and the driving passive pulley is fixedly installed on the output shaft 11. The driving belt is connected to the driving active pulley and the driving passive pulley.

[0069] The driving motor 81 drives the driving shaft to rotate, the driving shaft drives the driving active pulley to rotate, the driving active pulley drives the driving belt to move, the driving belt drives the driving passive pulley to rotate, and the driving passive pulley drives the output shaft 11 to rotate.

[0070] Embodiment 8, refer to Figure 1-Figure 14 The difference from the above embodiment is that a sliding sleeve 20 is fixedly installed on the top outside of the swing sleeve 31, and the sliding sleeve 20 slides in the storage hole. A plurality of clamping blocks 10 are fixedly installed in the swing sleeve 31, and the clamping blocks 10 are made of silicone.

[0071] When the swing sleeve 31 moves vertically in the storage hole, the swing sleeve 31 drives the sliding sleeve 20 to move, and the sliding sleeve 20 moves vertically in the storage hole to make the swing sleeve 31 more stable. The container 30 is inserted into the swing sleeve 31 and clamped by the clamping block 10.

[0072] Embodiment 9, refer to Figure 1-Figure 14 , which is different from the above-mentioned embodiment, is that the fixing rod 51 passes through the oscillation frame 13 and is slidably matched with the oscillation frame 13 , and the bottom of the fixing rod 51 is fixedly mounted on the oscillation plate 26 , and the oscillation plate 26 is slidably matched in the oscillation housing 9 .

[0073] The oscillation plate 26 drives the fixing rod 51 to move vertically, and the fixing rod 51 drives the pressing plate 53 to move vertically, so that the pressing plate 53 can move vertically with the container 30 to press the top of the container 30, and the pressing effect on the container 30 is better.

[0074] A mixing method for cell culture treatment, using the above-mentioned oscillating mixing device, comprises the following steps:

[0075] Adding cell culture medium into container 30, adding a specified amount of cells into container 30;

[0076] Place the container 30 in the swing sleeve 31, press the pressing plate 53 against the top of the container 30, rotate the locking cylinder 55, and move the locking cylinder 55 toward the pressing cylinder 56. At the same time, the locking cylinder 55 pushes the locking plate 52 to move between the two fixed blocks 54, and locks the pressing plate 53 against the top of the container 30.

[0077] The output shaft 11 drives the oscillation shaft 12 to move in a circle, the oscillation shaft 12 rotates eccentrically relative to the output shaft 11, the oscillation shaft 12 drives the oscillation frame 13 to move, the oscillation frame 13 drives the swing sleeve 31 to move, the swing sleeve 31 drives the container 30 to move in a circle, the liquid in the container 30 rotates in one direction to form a vortex, and the cells and cell culture medium in the liquid are mixed;

[0078] When the oscillation shaft 12 moves in a circular motion, the vertical oscillation component 2 drives the swing sleeve 31 to move vertically periodically, and the swing sleeve 31 drives the container 30 to move vertically periodically, so that the liquid in the container 30 is exchanged with each other in the vertical direction, and the cells and cell culture medium in the liquid are further mixed;

[0079] When the swing sleeve 31 moves vertically periodically, it drives the swing rod 32 to move vertically periodically. When the swing rod 32 moves upward in the swing groove 4, the inclined swing groove 4 causes the swing rod 32 to rotate, and the swing rod 32 drives the swing sleeve 31 to rotate. When the swing rod 32 moves downward in the swing groove 4, the swing rod 32 rotates in the opposite direction, so that the swing sleeve 31 continuously swings with the periodic vertical movement, so that the liquid in the container 30 continuously changes the rotation direction, so that it is mixed more evenly.

[0080] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An oscillating mixing device, comprising a vortex oscillating component, a vertical oscillating component and a monomer swinging component, characterized in that: The vortex oscillation assembly includes an output shaft, an oscillation shaft and an oscillation frame. When the output shaft rotates, it can drive the oscillation shaft to move in a circle, and the oscillation shaft can drive the oscillation frame to move. The oscillation frame is provided with a plurality of storage holes, the single body swing assembly is arranged in the storage holes, the single body swing assembly comprises a swing sleeve and a swing rod, the swing sleeve is arranged in the storage hole, the swing rod is rotatably connected to the outer wall of the swing sleeve, a swing groove is provided in the storage hole, the swing groove is inclined, and the swing rod is slidably fitted in the swing groove; When the oscillation shaft moves in a circular motion, the vertical oscillation assembly can cause the swing sleeve to move vertically periodically; The vertical oscillation assembly is provided with a top cover clamping assembly, and the top cover clamping assembly includes a fixing rod, a locking plate, a clamping plate, a plurality of fixing blocks, a locking cylinder and a clamping cylinder. The locking cylinder is fixedly mounted on the clamping plate. Limiting grooves are provided on both sides of the fixing rod. A plurality of fixing blocks are fixedly mounted in the limiting grooves at equal distances. The clamping cylinder is threadedly connected in the locking cylinder. When the clamping cylinder moves into the locking cylinder, the locking plate can be pushed between two adjacent fixing blocks. When the swing sleeve moves vertically, it can drive the fixed rod to move vertically; The oscillation frame is fixedly mounted on the oscillation housing, the vertical oscillation assembly includes a moving cylinder, the oscillation shaft is rectangular, the inner wall of the moving cylinder is rectangular, the moving cylinder is sleeved on the oscillation shaft and slidably cooperates with the oscillation shaft, and the moving cylinder penetrates the oscillation housing and slidably cooperates with the oscillation housing; The top cover pressing assembly also includes a limit block, a rotating ring and a push plate, wherein the limit block is fixedly mounted on the inner wall of the locking cylinder, one end of the locking plate is rotatably connected to the limit block, the rotating ring is fixedly mounted on the inner wall of the locking cylinder, one end of the push plate is rotatably connected to the rotating ring, and the other end of the push plate is rotatably connected to the locking plate; The top cover clamping assembly also includes a rotating block, the limiting block is slidably fitted in the limiting groove, the clamping plate is provided with a clamping groove corresponding to the storage hole, the rotating block is fixedly mounted on the outer wall of the locking cylinder, and the locking cylinder is sleeved on the fixing rod; The oscillation shaft is eccentrically arranged with respect to the output shaft, and the vortex oscillation assembly further comprises an output disk, an oscillation disk, an adjustment stud and an adjustment nut, wherein the output shaft is fixedly mounted at the center of the output disk, the oscillation shaft is fixedly mounted at the center of the oscillation disk, the adjustment stud is fixedly mounted on the output disk, an adjustment bar hole is provided on the oscillation disk, the oscillation shaft passes through the adjustment bar hole, the adjustment nut is sleeved on the adjustment stud and is threadedly connected with the adjustment stud, and the adjustment nut is against the oscillation disk; The vortex oscillation assembly further includes a plurality of limit teeth and a clamping block. A connecting groove is provided on the output disk. The plurality of limit teeth are fixedly installed in the connecting groove. The clamping block is fixedly installed at the bottom of the oscillation disk and slidably fits in the connecting groove. The clamping block cooperates with the limit teeth. A fixed shell is arranged below the oscillation shell, and the vertical oscillation assembly comprises an oscillation spring, a moving block, a moving ring, a moving rod and an oscillation plate, wherein the moving block is fixedly mounted on the moving cylinder, the moving rod is rotatably connected to the moving block, the moving ring is fixedly mounted on the fixed shell, and release grooves are equidistantly provided on the moving ring, one end of the oscillation spring is fixedly mounted on the moving block, and the other end of the oscillation spring is fixedly mounted on the oscillation shell, the oscillation spring is sleeved on the moving cylinder, the top of the moving cylinder is fixedly mounted on the oscillation plate, and the swing sleeve is rotatably mounted on the oscillation plate; A sliding sleeve is fixedly installed on the top of the swing sleeve, and the sliding sleeve is slidably fitted in the storage hole. A plurality of clamping blocks are fixedly installed in the swing sleeve, and the clamping blocks are made of silicone. The fixing rod passes through the vibration frame and is slidably matched with the vibration frame. The bottom of the fixing rod is fixedly mounted on the vibration plate, and the vibration plate is slidably matched in the vibration housing.

2. A vibration mixing device according to claim 1, characterized in that: The four corners of the bottom of the oscillation shell are each provided with a stabilizing component, and the stabilizing component includes a rotating shaft, a sliding guide rail, a sliding block and a stabilizing shaft. The rotating shaft is rotatably connected to the fixed shell, the sliding guide rail is fixedly installed on the rotating shaft, the sliding block is slidably fitted on the sliding guide rail, the stabilizing shaft is fixedly installed on the sliding block, and the top of the stabilizing shaft is rotatably connected to the bottom of the oscillation shell.

3. The oscillating mixing device according to claim 1, characterized in that: A driving assembly is arranged in the fixed housing, and the driving assembly can drive the output shaft to rotate. The driving assembly includes a driving motor, a driving shaft and a driving belt member. The driving motor is fixedly installed in the fixed housing, and the driving shaft is fixedly installed at the output end of the driving motor. The driving belt member includes a driving active pulley, a driving passive pulley and a driving belt. The driving active pulley is fixedly installed on the driving shaft, and the driving passive pulley is fixedly installed on the output shaft. The driving belt is connected to the driving active pulley and the driving passive pulley.

4. A mixing method for cell culture treatment, using the oscillating mixing device according to any one of claims 1 to 3, characterized in that: The following steps are involved: adding cell culture medium to the container, adding a specified amount of cells to the container; Place the container in the swing sleeve, press the pressing plate against the top of the container, rotate the locking cylinder, the locking cylinder moves toward the pressing cylinder, and at the same time the locking cylinder pushes the locking plate to move between the two fixed blocks, locking the pressing plate against the top of the container; The output shaft drives the oscillation shaft to move in a circle, the oscillation shaft rotates eccentrically relative to the output shaft, the oscillation shaft drives the oscillation frame to move, the oscillation frame drives the swing sleeve to move, the swing sleeve drives the container to move in a circle, the liquid in the container rotates in one direction to form a vortex, and the cells and cell culture medium in the liquid are mixed; When the oscillation shaft moves in a circular motion, the vertical oscillation component drives the swing sleeve to move vertically periodically, and the swing sleeve drives the container to move vertically periodically, so that the liquid in the container is mutually exchanged in the vertical direction, and the cells and cell culture medium in the liquid are further mixed; When the swing sleeve moves vertically periodically, it drives the swing rod to move vertically periodically. When the swing rod moves upward in the swing groove, the inclined swing groove causes the swing rod to rotate, and the swing rod drives the swing sleeve to rotate. When the swing rod moves downward in the swing groove, the swing rod rotates in the opposite direction, so that the swing sleeve continuously swings with the periodic vertical movement, so that the liquid in the container continuously changes its rotation direction, thereby being mixed more evenly.

Images