An integrated microbial generation device, mobile deployment vehicle and method

Abstract

The present invention relates to an integrated microbial generation device, a mobile deployment vehicle, and a method, wherein the generation device includes an inlet water pretreatment module, a water quality monitoring and control module, an algae cultivation module, and a zooplankton expansion module, which are assembled in a box and connected in sequence. The algae cultivation module includes a multi-channel parallel pipeline algae cultivation component. The zooplankton expansion module includes an expansion tank, which is connected in sequence to a zooplankton capture component and a two-way pump through an external transmission pipeline. The inlet water pretreatment module, the water quality monitoring and control module, the algae cultivation module, and the zooplankton expansion module are all electrically connected to an integrated controller. The present invention has developed a set of microbial generation devices that connect in series the inlet water pretreatment, algae expansion, zooplankton expansion, and release. The device can be used for short-term closed cultivation for storage, transportation, and transfer, and can be deployed for long-term continuous expansion at a fixed point. It has good flexibility and can output algae and provide sufficient living zooplankton for aquaculture, water environment improvement, and water ecological restoration.

Description

Technical Field

[0001] The present invention belongs to the field of algae microorganism cultivation, and relates to a co-cultivation device for algae and zooplankton. The device can transport algae outward as needed or cultivate and transport zooplankton outward using the proliferated algae as a food source. The device specifically relates to an integrated microorganism generation device, a mobile deployment vehicle and a method. Background Art

[0002] Algae, mostly microorganisms visible only through a microscope, are photoautotrophic organisms capable of photosynthesis, using light energy to convert inorganic matter into organic matter. In natural aquatic ecosystems, algae are important primary producers and a key link in the aquatic food chain. They influence water productivity, material transformation, and energy flow, ultimately maintaining the natural ecological balance of the water. Excessive algal growth (e.g., blooms or red tides) can impart odor and toxicity to water, degrading water quality. Therefore, in water bodies at risk of algal overgrowth, large quantities of algae-feeding zooplankton can be introduced to limit algal growth. Furthermore, the mutualistic relationship between algae and bacteria can be exploited for wastewater purification, where algae play a crucial role in the complex self-purification process of water. Therefore, in some wastewater ponds, turbid water ponds, or freshwater ponds after pond cleaning, algae are introduced to promote their growth to purify or restore the water ecosystem.

[0003] Zooplankton is a general term for small, heterotrophic, weakly swimming plankton, microorganisms that live in water. Zooplankton primarily feed on algae, bacteria, and organic particles, and are also preyed upon by other zooplankton, fish larvae, and other filter-feeding aquatic animals. Therefore, they occupy a central position in the flow of matter, energy, and information in aquatic ecosystems. Small and nutritious, zooplankton are a crucial starter food for fish larvae and juveniles. Consuming zooplankton significantly improves survival and promotes growth and development. Therefore, providing sufficient zooplankton as bait is essential for fish larvae and juveniles, especially those of rare and valuable species. Furthermore, zooplankton can effectively remove suspended matter such as algae, bacteria, and organic debris from water bodies, efficiently recover nutrients such as carbon, nitrogen, and phosphorus, significantly improve water clarity, create habitats for submerged plants, inhibit algal blooms caused by eutrophication, and maintain the material and energy transfer pathways of aquatic ecosystems. Therefore, they are also crucial for aquatic environmental protection and ecological restoration. However, the demand for zooplankton in aquaculture, water environment improvement and water ecological restoration is large and relatively seasonal. For example, the density of rotifers, an important starter bait for larvae and juveniles, is required to reach 10-20 / mL, while algae blooms generally occur in summer. The natural supply is often unable to meet the high-density, seasonal, temporary and emergency needs. In addition, due to limitations of site, facilities or landscape factors, it is often difficult to carry out expansion operations in situ or adjacent to the target water body.

[0004] Therefore, based on the above situation, it is necessary to develop an integrated microbial generation device and a mobile deployment vehicle and method containing the same, so as to enable algae culture or zooplankton culture function and deliver algae or zooplankton into the water body when needed. Summary of the Invention

[0005] The present invention provides an integrated microbial generation device, a mobile deployment vehicle and a method, aiming to at least to some extent overcome the shortcomings of conventional expansion and cultivation equipment in the prior art, such as the difficulty in automatically achieving the co-cultivation of algae and zooplankton, and the inability to switch functions and selectively supply algae and zooplankton according to the specific needs of aquaculture, water environment improvement and water ecological restoration.

[0006] The technical solution of the present invention to solve the above technical problems is as follows: an integrated microbial generation device (or platform or system), which includes an inlet pretreatment module, a water quality monitoring and control module, an algae cultivation module and a zooplankton expansion and cultivation module which are assembled in a box and connected in sequence. The inlet pretreatment module is used to take water from the target water body and transport it to the water quality monitoring and control module after pretreatment. The water quality monitoring and control module is used to detect the pretreated water and select additional agents for control according to water quality indicators to obtain a solution suitable for algae cultivation. The algae cultivation module includes multiple parallel pipeline algae cultivation components, and each pipeline algae cultivation component includes a transparent algae cultivation module. The algae cultivation pipeline and the LED light arranged outside the algae cultivation pipeline, the liquid inlet end of the algae cultivation pipeline is connected to the water quality monitoring module, and the liquid outlet end is connected to the algae liquid discharge pipeline and the zooplankton expansion module through a monitoring and supply component, the monitoring and supply component includes a feed pump and an algae liquid sampling and detection mechanism connected in parallel with the feed pump, the zooplankton expansion module includes an expansion tank, and the expansion tank is connected to the zooplankton capture component and the two-way pump in sequence through an external transmission pipeline, and a plankton sampling and detection mechanism is provided on the expansion tank or the external transmission pipeline, and the water pretreatment module, water quality monitoring and control module, algae cultivation module and zooplankton expansion module are respectively electrically connected to the integrated controller.

[0007] On the basis of the above technical solution, the present invention can also be improved as follows.

[0008] Furthermore, the inlet water pretreatment module includes a one-way pump, a pressure sensor, a filter component and an ultraviolet disinfection component which are sequentially connected through an inlet pipe.

[0009] The unidirectional pump draws water from target water bodies such as rivers, lakes, ponds, and pools, or can be connected directly to tap water or groundwater. The incoming water passes through a filtration assembly (preferably a 100μm filter) to remove large suspended solids and undergoes ultraviolet disinfection before being delivered to an online water quality monitoring module. An inlet pressure sensor provides feedback on the pipeline pressure to the integrated controller, which intelligently adjusts the unidirectional pump power according to a pre-programmed procedure. Excessive pressure signals a prompt to replace the filter assembly.

[0010] Furthermore, the water quality monitoring and control module includes a water quality online monitoring component 1, an automatic dosing component and a liquid storage tank that are connected in sequence, and the outlet of the liquid storage tank is connected to the inlet end of each algae cultivation pipeline through a liquid inlet pump.

[0011] Online water quality monitoring primarily covers indicators such as dissolved oxygen, pH, chemical oxygen demand, ammonia nitrogen, turbidity, total nitrogen, and total phosphorus. For influent water deficient in elements such as carbon, nitrogen, and phosphorus, the integrated control module can supplement these elements with solutions such as sodium carbonate, sodium nitrate, and dipotassium hydrogen phosphate via a dosing component. Furthermore, the dosing component can also be used to add microorganisms beneficial to algae and zooplankton, such as nitrifying bacteria, photosynthetic bacteria, and Bacillus, to the water to promote their growth and reproduction. After dosing, the influent is temporarily stored in a liquid reservoir before being transferred to the algae cultivation module.

[0012] Furthermore, the liquid inlet pump is arranged on the liquid inlet main pipe, one end of the liquid inlet main pipe is connected to the liquid storage tank, and the inlet end of each algae cultivation pipe is connected to the other end of the liquid inlet main pipe through a corresponding liquid inlet branch pipe equipped with an electric throttle valve.

[0013] Furthermore, a temperature and humidity control module is provided in the box, and the temperature and humidity control module includes a temperature and humidity sensor and an air conditioner.

[0014] The temperature and humidity sensors are responsible for obtaining temperature and humidity information in real time and feeding it back to the intelligent integrated controller, which controls the air conditioner (air conditioner) to adjust the temperature and humidity of the algae and zooplankton cultivation environment.

[0015] Furthermore, the algae cultivation pipeline adopts a spiral winding or reciprocating folding design, and the algae liquid sampling and detection mechanism includes a sampling pump, a plankton density monitor, and a chlorophyll fluorescence meter. After the algae species are inoculated in the algae cultivation pipeline, a two-way pump inputs the dosing water in the liquid reservoir into the algae cultivation pipeline. The algae cultivation pipeline adopts a multi-way parallel design, and the LED light source is arranged in the gap between the pipelines. The LED light source uses a full-spectrum white light source, which can be turned on and off at a fixed time and the light intensity can be adjusted. Different algae species can be inoculated in the parallel pipelines to meet the diverse nutritional needs of the cultured zooplankton. Each pipeline is equipped with components such as a two-way throttle valve, a liquid level sensor, a one-way pump, a pressure pump, a plankton density monitor, and a chlorophyll fluorescence meter. While ensuring independent operation, it can also coordinate the differential growth characteristics of different algae species by adjusting the inlet and outlet water. Furthermore, the independent pipelines prevent cross-contamination and allow for flexible restarting of culture lines in the event of algae strain degradation or species change. This drained water is pumped back into a reservoir by a bidirectional pump for harmless treatment and subsequent discharge. Each algae culture line can be connected to a dedicated transfer tank via a valved bypass. This allows for draining, cleaning, and re-inoculation of a specific algae culture line in the event of adverse conditions such as sluggish algae growth or mass algae die-off, or for reusing algae liquid during normal cultivation.

[0016] Algae density and growth are monitored in real time using a plankton density monitor and / or a chlorophyll fluorometer. Specifically, a sampling pump (1), a microfluidic density monitor, and a chlorophyll fluorometer are connected in series to form a parallel bypass of the algae output pipeline. Sampling pump (1) accurately delivers a tiny volume of algae liquid from the algae culture pipeline to the microchannel. The density monitor then counts the algae and calculates their density. The chlorophyll fluorometer measures the chlorophyll fluorescence characteristics in the algae cells, reflecting the photosynthetic efficiency and physiological status of the algae. The monitoring results are uploaded to the intelligent integrated controller in real time.

[0017] The output of the algae product is regulated by a liquid supply pump connected to the end of the pipeline. The output timing and volume are dynamically adjusted by an intelligent integrated controller, which takes into account parameters such as the liquid level in the pipeline, algae density and growth status, zooplankton density downstream, the liquid level in the expansion tank, and the density of remaining ungrazed algae. Liquid level sensors can be installed at necessary locations in the algae cultivation pipeline, expansion tank, and liquid storage tank.

[0018] Furthermore, the expansion culture pond is provided with an aeration and oxygenation component and a second water quality online monitoring component, and the plankton sampling and detection mechanism includes a second sampling pump and a plankton online monitor.

[0019] Oxygenation equipment (aeration and oxygenation component) and water quality online monitoring equipment (water quality online monitoring component 2) are installed inside the zooplankton expansion tank, focusing on monitoring indicators such as water temperature, pH, dissolved oxygen, and ammonia nitrogen. A liquid level sensor can be installed in the expansion tank to provide real-time feedback on the liquid level in the expansion tank to the intelligent integrated controller. The plankton sampling and detection mechanism can be composed of a sampling pump 2 and a plankton online monitor (specifically, a microfluidic density monitor) connected in series to form a parallel bypass of the zooplankton output pipeline. The sampling pump 2 accurately transports the tiny volume of liquid in the zooplankton culture tank to the microchannel, and the suspended particles are subdivided into algae and zooplankton through multi-channel particle size diversion. The density monitor then counts and calculates the density of the algae and zooplankton respectively. The calculation results are uploaded to the intelligent integrated controller in real time to determine the output time and output of zooplankton.

[0020] Furthermore, the zooplankton capturing assembly includes a hard cylinder and a plankton net arranged in the cylinder, the thick end of the plankton net is fixed to the inlet end of the cylinder, the thin end of the plankton net is provided with an electric valve and is fixed to the outlet end of the cylinder, and the two ends of the cylinder are detachably connected with end covers with joints, and the end covers are connected to the external transmission pipeline through the joints; there are multiple zooplankton capturing assemblies and they are arranged in series, and the mesh size of the plankton nets of the multiple zooplankton capturing assemblies decreases successively along the water flow direction to capture zooplankton of different body lengths or types.

[0021] The zooplankton capture component preferably uses a No. 25 plankton net, but other aperture nets can also be selected according to the purpose. When the zooplankton density is high, the capture process can be omitted and the zooplankton can be directly output to the target water body. When the culture water from the zooplankton expansion tank containing zooplankton products flows through the No. 25 plankton net, the zooplankton is concentrated and captured on the net wall. At this time, the valve behind the net bag is opened to output the concentrated zooplankton product, which can be collected and temporarily stored in a container or delivered to the target water body at a fixed point through a pipeline. A pressure sensor can be installed on the external transmission pipeline to monitor the pressure of the pipeline where the capture net is located and upload the data in real time to the intelligent integrated controller, which opens and closes the net bag valve at the right time and controls the two-way pump to backwash the plankton attached to the net wall to keep the mesh unobstructed for continuous production.

[0022] The zooplankton collection component is provided with a collection net or a combination net with an appropriate aperture (multiple plankton nets are connected in series, specifically, multiple plankton nets are sequentially arranged in a cylinder, and when the valves at the thin ends of each plankton net are opened, zooplankton of all sizes can be discharged). The purpose is: (1) in the case of mixed culture, zooplankton species of different body lengths can be collected according to the aperture of the net and released separately or in sequence; (2) in the case of single culture, the size difference of individuals at different ages or developmental stages can be used to distinguish between young and adult individuals by adjusting the aperture of the net. The juveniles are filtered and output to the target water body, saving feeding algae, and the adults are returned to the zooplankton expansion module through backwashing to maintain high productivity of the platform.

[0023] As previously mentioned, the real-time data collected by each module of the integrated microbial generation device provided by the present invention is aggregated into an intelligent integrated controller, which coordinates and controls the data to maintain optimal system synergy and maximize production efficiency. Specific control can be achieved through pre-setting corresponding parameters and programming for programmatic automatic control. This includes: water inlet time and flow rate, filtration module pressure, UV disinfection duration, water quality monitoring, chemical addition, water reservoir level, two-way throttle valve flow rate and direction, LED light intensity, monitoring of liquid level, density, and physiological status in the algae culture pipeline and zooplankton culture tank, temperature and humidity control, zooplankton feeding, capture, and release, and water outlet and backwash time and flow rate.

[0024] The device provided by the present invention can be equipped with a variety of sensors and online monitoring equipment. All data are uploaded to the integrated controller, which adjusts the temperature, humidity, pressure, light, liquid level, water quality, algae and zooplankton density and input and output parameters in the system in real time, and controls the running direction and speed of the water pump motor, so that the environment in the system is always in the optimal conditions for algae and zooplankton production, greatly improving the utilization efficiency of energy, water resources and space resources, and saving manpower and material costs.

[0025] The present invention also provides an integrated microorganism generation mobile deployment vehicle, which includes a vehicle body with its own walking power, the vehicle body is a vehicle or a ship, and the vehicle or ship is detachably loaded with the above-mentioned integrated microorganism generation device.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The present invention has developed an integrated mobile deployment device (platform or system) that connects water pretreatment, algae cultivation, zooplankton cultivation, and release. This device can be used for both short-term closed cultivation for storage, transportation, and transfer, as well as long-term, fixed-point deployment for continuous cultivation. This highly flexible device solves the problems of algae and zooplankton cultivation, storage, transportation, and release, providing sufficient living zooplankton for aquaculture, water environment improvement, and water ecological restoration. It can also cultivate and export algae individually when needed in specific water bodies or situations. Multiple parallel algae cultivation pipelines can produce different species and / or algae in different growth cycles, satisfying the need to cultivate and export diverse algae while also providing a sustainable and stable supply of diverse algae food for the zooplankton in the cultivation pool.

[0028] The integrated microbial generation device provided by the present invention can be loaded onto vehicles such as ships and can be flexibly deployed with the vehicles to the target water body for aquaculture or restoration, thereby realizing efficient and continuous expansion of zooplankton and rapid in-situ output to the target water body. The target water body is used to provide water sources and local zooplankton communities or individuals. After expansion, living zooplankton and relatively cleaner water are directly output to the target water body, avoiding the introduction of foreign genotypes, avoiding pollution of the local zooplankton gene pool and continuously purifying the water quality. The mobile scene and the fixed-point deployment scene can be switched flexibly: in the mobile scene, the water inlet and outlet are both closed, and the light intensity and temperature are adjusted by the integrated controller to reduce the metabolic rate of algae and zooplankton, thereby achieving short-term stability and self-sustainability (about 7 days), which is convenient for long-distance and large-scale conversion of deployment positions, and also convenient for the vehicle to be transferred to a specific place nearby for refueling and powering after fixed-point deployment; in fixed-point deployment, water inlet and outlet can be carried out continuously or intermittently according to the set program (water inlet and outlet can be carried out asynchronously). After the inlet water is filtered and ultraviolet disinfected and passes the test, the water quality test is carried out and nutrients such as carbon, nitrogen, phosphorus and beneficial bacteria are added according to the test results, and then used for algae expansion. The produced algae products are automatically supplied as food to the zooplankton expansion module after passing the instrument test. After the zooplankton expansion meets the standards, they are directly released to the target water body nearby or enriched and then released to a specific location of the target water body through a pipeline. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 A schematic diagram showing the structure of the integrated microbial generation device provided by the present invention in modules;

[0030] Figure 2 for Figure 1 A schematic diagram showing the connection relationship between the specific components of the generating device;

[0031] Figure 3 The present invention provides Figure 1 Schematic diagram of the mobile deployment vehicle (vehicle) of the integrated microorganism generation device shown.

[0032] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0033] 100, water pretreatment module; 200, water quality monitoring and control module; 300, algae cultivation module; 400, zooplankton expansion module; 500, integrated controller; 600, temperature and humidity control module;

[0034] 101. One-way pump; 102. Pressure sensor; 103. Filter assembly; 104. UV disinfection assembly;

[0035] 201. Water quality online monitoring component 1; 202. Automatic dosing component; 203. Liquid storage tank; 204. Liquid inlet pump;

[0036] 301. Algae culture pipe; 302. LED lamp; 303. Feed pump; 304. Electric throttle valve; 305. Sampling pump 1; 306. Plankton density monitor; 307. Chlorophyll fluorescence meter;

[0037] 401. Expansion culture tank; 402. Zooplankton collection assembly; 403. Bidirectional pump; 405. Aeration and oxygenation assembly; 406. Water quality online monitoring assembly 2; 407. Sampling pump 2; 408. Plankton online monitor. DETAILED DESCRIPTION

[0038] The principles and features of the present invention are described below with reference to the accompanying drawings and specific embodiments. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0039] In the description of the present invention, if terms indicating directions such as "up", "down", "left", "right", "top", "bottom", "inside" and "outside" are used, the directions or positional relationships indicated are based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operate in a specific direction. Therefore, they should not be understood as limiting the present invention.

[0040] like Figure 1 and 2As shown, the present invention provides an integrated microbial generation device, which includes an inlet water pretreatment module 100, a water quality monitoring and control module 200, an algae cultivation module 300 and a zooplankton expansion and cultivation module 400 which are assembled in a box and connected in sequence. The inlet water pretreatment module 100 is used to take water from the target water body and transport it to the water quality monitoring and control module 200 after pretreatment. The water quality monitoring and control module 200 is used to detect the pretreated water and select and add a supplementary agent according to the water quality index to obtain a solution suitable for algae cultivation. The algae cultivation module 300 includes multiple parallel pipeline algae cultivation components, each of which includes a transparent algae cultivation pipeline 301 and an LED lamp 303 provided outside the algae cultivation pipeline 301. 02. The liquid inlet end of the algae cultivation pipeline 301 is connected to the water quality monitoring and control module 200, and the liquid outlet end is connected to the algae liquid discharge pipeline and the zooplankton expansion module 400 through a monitoring and supply component. The monitoring and supply component includes a feed pump 303 and an algae liquid sampling and detection mechanism connected in parallel with the feed pump 303. The zooplankton expansion module 400 includes an expansion tank 401. The expansion tank 401 is connected to the zooplankton capture component 402 and the two-way pump 403 in sequence through an external transmission pipeline. A plankton sampling and detection mechanism is provided on the expansion tank 401 or the external transmission pipeline. The inlet pretreatment module 100, the water quality monitoring and control module 200, the algae cultivation module 300 and the zooplankton expansion module 400 are respectively electrically connected to the integrated controller 500.

[0041] The inlet water pretreatment module 100 includes a one-way pump 101, a pressure sensor 102, a filter assembly 103, and a UV disinfection assembly 104, which are sequentially connected via an inlet pipe. The water quality monitoring and control module 200 includes an online water quality monitoring assembly 201, an automatic dosing assembly 202, and a liquid reservoir 203, which are sequentially connected. The outlet of the liquid reservoir 203 is connected to the inlet of each algae cultivation pipe 301 via a pipe equipped with an inlet pump 204. The inlet pump 204 is installed on the main inlet pipe, one end of which is connected to the liquid reservoir 203. The inlet of each algae cultivation pipe 301 is connected to the other end of the main inlet pipe via a corresponding inlet branch pipe equipped with an electric throttle valve 304. The housing also contains a temperature and humidity control module 600, which includes a temperature and humidity sensor and an air conditioner. The algae cultivation pipeline 301 adopts a spiral winding or reciprocating folding design. The algae liquid sampling and detection mechanism includes a sampling pump 1 305, a plankton density monitor 306, and a chlorophyll fluorescence meter 307. The algae liquid discharge pipeline is equipped with an electric valve, which is opened only when the algae liquid needs to be withdrawn or discharged. The expansion tank 401 is equipped with an aeration and oxygenation component 405 and a second online water quality monitoring component 406. The plankton sampling and detection mechanism includes a sampling pump 2 407 and an online plankton monitor 408.

[0042] The present invention also provides an integrated microorganism generation mobile deployment vehicle, which includes a vehicle body with self-powered walking, the vehicle body is a vehicle or a ship, and the vehicle or ship is detachably loaded with the above-mentioned integrated microorganism generation device. When the vehicle body is a vehicle, the integrated microorganism generation mobile deployment vehicle is as follows Figure 3 As shown, the integrated microorganism generating device is a detachable container body placed on the vehicle chassis when viewed from the outside.

[0043] In order to meet the light transmission requirements of the algae and zooplankton expansion modules, as well as the need for vehicle mobility, the algae expansion module is made of a pipe structure with colorless transparent organic glass, and the zooplankton expansion module is made of a cube structure with tempered glass or colorless transparent organic glass. Wave-breaking plates are required to be installed in both the pipe and the cube to enhance the structural strength while weakening the water hammer effect caused by liquid inertia. For the specific design specifications of the wave-breaking plates, please refer to GB 18564.1-2019.

[0044] The present invention also provides a method for rapidly expanding and releasing zooplankton using the above-mentioned integrated microbial generation mobile deployment vehicle, which specifically includes the following steps:

[0045] S1. The integrated microbial generation device is transported to a designated location in the target water body via a vehicle. The water pretreatment module 100 and the water quality monitoring and control module 200 are activated. Water is drawn from the target water body, filtered, disinfected, and dosed to obtain a solution suitable for algae cultivation. The solution is then transported to the liquid storage tank 203 for standby use.

[0046] S2. Algae are inoculated into each algae cultivation pipe 301 , and the liquid supply pump is activated to deliver the solution in the liquid reservoir 203 to each algae cultivation pipe 301 . The LED light 302 is activated to initiate algae cultivation and growth. The algae density and growth status in each algae cultivation pipe 301 are monitored by an algae liquid sampling and detection mechanism, and the monitoring results are fed back to the integrated controller 500 . The integrated controller 500 controls the start and stop times and flow rates of each liquid supply pump to deliver the cultured algae liquid in the algae cultivation pipe 301 to the expansion tank 401 .

[0047] S3. While culturing algae, collect zooplankton from the target water body. Select some active target zooplankton individuals under a dissecting microscope, and after pre-culturing and multiplying them to the required number, inoculate them into the expansion culture tank 401. Based on actual needs, you can choose to inoculate local zooplankton communities or select a certain type of target species based on morphological characteristics (such as body length, etc.), or cultivate a specific species separately. For example, the initial bait demand of larvae and juveniles is mainly small rotifers, the demand for water body restoration is mainly medium and large cladocerans, and the marine zooplankton is mainly copepods. The specific process of zooplankton selection is as follows: (1) Germplasm screening: Use a No. 25 plankton net to collect local communities, and absorb target zooplankton under a dissecting microscope as needed, giving priority to individuals with strong vitality and eggs, with the number of each individual being no less than 100; (2) Preliminary expansion: Use a 10L small system for preliminary expansion, feed sufficient algae every day, and control the temperature and dissolved oxygen within an appropriate range; (3) Batch inoculation: After the zooplankton in the expansion reaches a certain number (for example, no less than 5,000), transfer it to the expansion module, and then carry out integrated expansion;

[0048] The zooplankton in the expansion and cultivation pool 401 feeds on the algae in the algae liquid transported by the algae cultivation pipeline 301 and proliferates rapidly. The algae density, zooplankton density and zooplankton growth status in the expansion and cultivation pool 401 are monitored by the plankton sampling and detection mechanism, and the monitoring results are fed back to the integrated controller 500. The integrated controller 500 controls the opening and closing of the electric valve of the zooplankton capture component 402 and the start and stop and flow rate of the bidirectional pump 403 to enrich the zooplankton after the expansion and cultivation meets the standards and then release them or directly release them to a specific location of the target water body. Subsequently, the integrated controller 500 controls the water pretreatment module 100, the water quality monitoring and control module 200, the algae cultivation module 300 and the zooplankton expansion and cultivation module 400 to continuously and automatically operate, so that the algae in the algae cultivation pipeline 301 continuously proliferate and are fed into the expansion and cultivation pool 401, thereby realizing the continuous automatic expansion and external transmission of the zooplankton in the expansion and cultivation pool 401.

[0049] Example 1

[0050] With the main purpose of improving the water environment and restoring the aquatic ecology, the zooplankton culture is set as freshwater large Daphnia (scientific name Daphnia magna, adult body length is about 3mm), and the on-board scenario of a 40-foot double-door standard container is used as an example.

[0051] The zooplankton generation platform is arranged in the container body, and the modules can be roughly arranged in the space inside the container as follows: the water inlet and pretreatment module is arranged on the left side inside the door, the three-way parallel pipeline algae culture component and the monitoring output module are respectively arranged on the left and right sides and the top of the box; the zooplankton expansion and monitoring output module is arranged at the bottom of the box; the intelligent integrated controller is arranged on the right side inside the door.

[0052] The inlet water is tap water or directly extracted from the target water body through a one-way water inlet pump. After filtration, disinfection, testing, and dosing, it is temporarily stored in a liquid storage tank. The three parallel algae culture pipes are inoculated with Chlorella vulgaris, S. obliquus, and Cyclotella meni respectively. The light intensity is adjusted to not less than 5000 Lux, the temperature is around 25 ° C, and the relative humidity is 40% to 60%. The bidirectional pump transports the dosing water in the liquid storage tank to the parallel culture pipes, and the water inlet time and flow rate are controlled by the bidirectional throttle valve. The microfluidic density monitoring component and chlorophyll fluorescence component at the end of the algae culture pipe respectively monitor the density and physiological state of the algae in the three parallel pipes. The density meets the standard (generally not less than 10 11 cells / m 3 )'s algae products are exported to the zooplankton expansion pond.

[0053] Using a No. 25 plankton net, zooplankton is collected from the target water body. At least 100 active Daphnia magna individuals carrying asexual eggs are selected under a dissecting microscope. These individuals are pre-cultured in a 10L micro-aquarium using the aforementioned algae products, maintained at a temperature of approximately 25°C and a relative humidity of 40% to 60%. Once the Daphnia magna population has grown to approximately 5,000 individuals, they are inoculated into a propagation tank for automated, intelligent propagation.

[0054] If the plankton density monitoring unit and chlorophyll fluorescence unit detect weak algae growth or poor physiological condition in a particular algae culture pipe, the intelligent integrated controller will prompt pipe cleaning, algae seed replacement, and reseeding. After manual confirmation, the one-way pump between the pipe and the expansion tank will stop operating, and the two-way throttle valves in the other two pipes will close. The two-way pump and the two-way throttle valve in the pipe will work together to empty the algae liquid in the pipe into a liquid storage tank for harmless treatment and discharge. The algae seed will then be reseeded. As a preferred option, each algae culture pipe can have a separate valved bypass connected to a dedicated transfer tank. If weak algae growth, poor physiological condition, or other special adverse conditions are detected, the bypass can be used to drain the liquid in the specific algae culture pipe to the transfer tank for treatment and discharge. Cleaning fluid is then injected into the waste liquid tank to clean the corresponding algae culture pipe through the bypass. Finally, the algae seed can be reseeded and the system can be put back into normal use. The transfer pool can also be used to flexibly access the algae liquid in one or some algae culture pipes, so that the device provided by the present invention has the function of outputting algae liquid.

[0055] The microfluidic density monitoring device at the end of the expansion tank regularly monitors the algae density and Daphnia magna density in the expansion tank. The liquid level sensor monitors the liquid level in the algae culture pipe and the expansion tank respectively ( Figure 1 The black dot in the middle represents the liquid level sensor. The intelligent integrated controller dynamically adjusts light intensity, temperature, algae feeding volume, and Daphnia magna dosage based on the algae level and density in the pipeline, the liquid level in the expansion tank, the number of Daphnia magna in the expansion tank, and the remaining algae density. Specifically, the relationship between algae density, feeding volume, and Daphnia magna density and volume is as follows:

[0056] ρ p ×V p =ρ z ×V z ×c+ρ p '×V z (1)

[0057] Where: ρ p ——Algae density in algae cultivation pipelines;

[0058] ρ p '——The density of algae in the expansion pool shall not be less than 5×10 9pcs / m 3 ;

[0059] ρ z ——The density of Daphnia magna in the expansion pond is not more than 5×10 5 pcs / m 3 ;

[0060] V p ——Volume of algae fed;

[0061] V z ——Volume of the expansion tank;

[0062] c is the algae consumption constant. Taking Chlorella as an example, an adult Daphnia magna consumes about 3×10 5 Chlorella.

[0063] The population growth rates of algae and Daphnia magna were calculated according to formula (2).

[0064] λ=(lnN t -lnN0) / t (2)

[0065] Where: λ——population growth rate

[0066] N0 – density at the start of monitoring;

[0067] N t —density at the end of monitoring,

[0068] t——Monitoring time interval.

[0069] The zooplankton capture component is equipped with a double-layer capture net in a sparse and dense order. The sparse net has a pore size of 10 meshes, which intercepts adult large Daphnia individuals for reproduction; the dense net has a pore size of 100 meshes, which intercepts young individuals for output.

[0070] In the maneuvering scenario, the load limit of a 40-foot double-door container is 28 tons. The liquid level of the zooplankton expansion tank is adjusted accordingly to meet the weight limit. At the same time, the light intensity is gradually reduced to below 500 Lux, the temperature is gradually lowered and controlled between 10℃ and 15℃, and the relative humidity is 40% to 60% to reduce the metabolism and reproduction rate of algae and zooplankton, which is convenient for long-distance storage and transportation. In this scenario, the volume of the expansion tank is 20m 3 The density of Daphnia magna is 5×10 5 pcs / m 3 Calculation shows that the maximum stock is no less than 10 million.

[0071] In the deployment scenario, the vehicle's hydraulic lifting legs are working to provide full support for the vehicle. At this time, the liquid level sensor is released and the platform is operating at full capacity. The light intensity is adjusted to no less than 5000 Lux, the temperature is around 25°C, and the relative humidity is 40% to 60%. In this scenario, the internal volume of a 40-foot double-door container is 67.5m 3 , the volume of the expansion pool is not less than 50m 3 Calculated based on the maximum number of Daphnia magna stocks, the maximum number of Daphnia magna stocks is not less than 25 million. Based on the intrinsic growth rate of Daphnia magna of 0.5 / d, the maximum daily production of Daphnia magna is not less than 12.5 million. 5 If we calculate the number of chlorella, the daily consumption of chlorella by Daphnia magna is nearly 4×10 12 Assuming that each Daphnia magna filters 200 mL of water per day and night, the volume of water filtered by Daphnia magna per day is no less than 2500 mL. 3 .

[0072] Example 2

[0073] Taking the provision of starter feed for aquaculture juvenile fish as an example, the zooplankton culture was set as the freshwater rotifer Brachionus calyciflorus (scientific name: Brachionus calyciflorus, body length approximately 300 μm), and the scenario was carried out in a 40-foot double-door standard container.

[0074] The layout of each module, water inlet and pretreatment, algae selection, lighting, temperature and humidity settings are as in Example 1. The difference is that the rotifer Brachionus calyciflorus is smaller, the population density it can maintain is higher, and the grazing rate and population growth rate are also different from those in Example 1. Therefore, V in formula (1) p ,ρ p ',ρ z , c, etc. will change accordingly. The intelligent integrated controller will dynamically adjust the parameters based on the data fed back by each sensor and online monitoring equipment.

[0075] In the mobile scenario, the volume of the expansion pool is 20m 3 The density of Brachionus calyciflorus was calculated as 2×10 7 pcs / m 3 Calculation shows that the maximum stock can reach 400 million. In the deployment scenario, the expansion pool volume is not less than 50m 3 Calculations indicate that the maximum stock of Brachionus calyciflorus should be no less than 1 billion individuals. Based on an intrinsic growth rate of 1.0 / day, the maximum daily production of Brachionus calyciflorus should be no less than 1 billion individuals. Assuming that each fish larvae and juvenile consumes an average of 50,000 Brachionus calyciflorus rotifers as they transition from predatory feeding to dietary transition, the daily production of Brachionus calyciflorus rotifers can provide predatory food for 20,000 fish larvae and juveniles.

[0076] Example 3

[0077] Taking the provision of starter feed for juvenile fish in marine ranches as an example, the zooplankton cultured was set to the marine rotifer Brachionus plicatilis (scientific name: Brachionus plicatilis, with an adult body length of about 300 μm), and the scenario of a 100,000-ton offshore closed aquaculture vessel was used as an example.

[0078] The order of arrangement of the modules can refer to that of Example 1, or it can be different from the box structure in Example 1, and instead adopt an open structure. In particular, to adapt to the operating environment conditions and water inlet and outlet volume of the offshore aquaculture vessel, the flux of corresponding facilities such as the water inlet and outlet controller, filtration module, dosing module, pipeline algae cultivation module, zooplankton expansion tank, and capture module are all increased in proportion. In addition, for the purpose of cost control and energy consumption reduction, some facilities can be omitted accordingly, such as the filtration module, ultraviolet disinfection module, temperature and humidity controller, etc., but equipment such as solar cells and underwater cleaning robots can be added.

[0079] Specifically, to fully utilize the three-dimensional space above the deck, the tubular algae cultivation module is configured with multiple rows of parallel pipes, with LED lighting and heating modules located in the intervals between the rows. Algae for expansion can be directly obtained from the surrounding water environment, or artificially added with species such as microalgae, Nannochloropsis, and Chlamydomonas.

[0080] The zooplankton expansion tank is sunken in the middle of the hull and can be flexibly designed into 2 to 4 tanks according to the hull structure. The total volume is 80,000 m 3 The density of Brachionus plicatilis was calculated as 2×10 7 pcs / m 3 Calculation, the maximum inventory can reach 1.6×10 12 According to the calculation of the inherent growth rate of the folding arm tail wheel at 1.0 / d, the maximum daily output is no less than 1.6×10 12 Assuming that each fish larvae and juvenile consumes an average of 50,000 Brachionus plicatilis rotifers from the time they start feeding on rotifers to the time they switch their diet, the daily production of Brachionus plicatilis rotifers can provide starter food for 32 million fish larvae and juveniles.

[0081] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An integrated microbial generation device, characterized in that: The invention comprises an inlet water pretreatment module (100), a water quality monitoring and control module (200), an algae cultivation module (300) and a zooplankton expansion and cultivation module (400) which are assembled in a box and are connected in sequence. The inlet water pretreatment module (100) is used to take water from a target water body and transport it to the water quality monitoring and control module (200) after pretreatment. The water quality monitoring and control module (200) is used to detect the pretreated water and select and add a supplementary agent according to the water quality index to obtain The algae culture module (300) comprises a plurality of parallel pipeline algae culture components, each of which comprises a transparent algae culture pipeline (301) and an LED lamp (302) arranged outside the algae culture pipeline (301), and each algae culture pipeline (301) is provided with a two-way throttle valve, a liquid level sensor, a one-way pump, a pressure pump, a plankton density monitor and a chlorophyll fluorescence meter to ensure that each algae culture pipeline The algae culture pipe (301) operates independently and can coordinate the differential growth characteristics of different algae species by adjusting the water inlet and outlet; the liquid inlet end of the algae culture pipe (301) is connected to the water quality monitoring and control module (200), and the liquid outlet end is connected to the algae liquid discharge pipe and the zooplankton expansion module (400) through the monitoring supply component, and the monitoring supply component includes a feed pump (303) and an algae liquid sampling and detection mechanism connected in parallel with the feed pump (303). The module (400) includes a culture expansion tank (401), the culture expansion tank (401) is connected to a zooplankton capture component (402) and a bidirectional pump (403) in sequence through an external transmission pipeline, the culture expansion tank (401) or the external transmission pipeline is provided with a plankton sampling and detection mechanism, and the inlet water pretreatment module (100), the water quality monitoring and control module (200), the algae cultivation module (300) and the zooplankton culture expansion module (400) are respectively electrically connected to an integrated controller (500); The zooplankton capturing component (402) includes a hard cylinder and a plankton net arranged in the cylinder, the thick end of the plankton net is fixed to the inlet end of the cylinder, the thin end of the plankton net is provided with an electric valve and is fixed to the outlet end of the cylinder, and the two ends of the cylinder are detachably connected with end covers with joints, and the end covers are connected to the external transmission pipeline through the joints; there are multiple zooplankton capturing components (402) and they are arranged in series, and the mesh size of the plankton nets of the multiple zooplankton capturing components (402) decreases successively along the direction of water flow to capture zooplankton of different body lengths or types.

2. An integrated microbial generation device according to claim 1, characterized in that: The inlet water pretreatment module (100) comprises a one-way pump (101), a pressure sensor (102), a filter component (103), and an ultraviolet disinfection component (104) which are sequentially connected through an inlet water pipe.

3. The integrated microorganism generation device according to claim 1, characterized in that: The water quality monitoring and control module (200) comprises an online water quality monitoring component (201), an automatic dosing component (202) and a liquid storage tank (203) which are connected in sequence, and the outlet of the liquid storage tank (203) is connected to the inlet end of each algae cultivation pipeline (301) through a pipeline provided with a liquid inlet pump (204).

4. An integrated microorganism generation device according to claim 3, characterized in that: The liquid inlet pump (204) is arranged on the liquid inlet main pipe, one end of the liquid inlet main pipe is connected to the liquid storage tank (203), and the inlet end of each algae cultivation pipe (301) is connected to the other end of the liquid inlet main pipe through a corresponding liquid inlet branch pipe provided with an electric throttle valve (304).

5. The integrated microorganism generation device according to claim 1, characterized in that: A temperature and humidity control module (600) is also provided in the box, and the temperature and humidity control module (600) includes a temperature and humidity sensor and an air conditioner.

6. The integrated microorganism generation device according to claim 1, characterized in that: The algae culture pipeline (301) adopts a spiral winding or reciprocating folding design, and the algae liquid sampling and detection mechanism includes a sampling pump (305), a plankton density monitor (306) and a chlorophyll fluorescence meter (307).

7. The integrated microorganism generation device according to claim 1, characterized in that: The expansion culture pond (401) is provided with an aeration and oxygenation component (405) and a second water quality online monitoring component (406), and the plankton sampling and detection mechanism includes a second sampling pump (407) and a plankton online monitor (408).

8. An integrated microbial generation mobile deployment vehicle, characterized in that: It comprises a carrier body with its own walking power, wherein the carrier body is a vehicle, and the integrated microorganism generating device according to any one of claims 1 to 7 is detachably loaded on the vehicle.

9. An integrated microbial generation method, characterized in that: The method of using the integrated microbial generation mobile deployment vehicle according to claim 8 to rapidly expand and release zooplankton comprises the following steps: S1. The integrated microbial generation device is transported to a designated location in the target water body via the vehicle body, and the water pretreatment module (100) and the water quality monitoring and control module (200) are activated. Water is taken from the target water body, filtered, disinfected, and regulated by adding drugs to obtain a solution suitable for algae cultivation, which is then transported to the liquid storage tank (203) for standby use. S2. Inoculate algae species into each algae culture pipe (301), start the liquid supply pump to transport the solution in the liquid storage tank (203) to each algae culture pipe (301), start the LED light (302), and start algae culture and proliferation. The algae density and growth status in each algae culture pipe (301) are monitored by the algae liquid sampling and detection mechanism, and the monitoring results are fed back to the integrated controller (500). The integrated controller (500) controls the start and stop and flow rate of each liquid supply pump to transport the cultured algae liquid in the algae culture pipe (301) to the expansion culture tank (401); S3. While the algae are being cultured, zooplankton are collected from the target water body. Some active target zooplankton individuals are picked up under a dissecting microscope. After pre-culture and proliferation until the number reaches the target, they are inoculated into the expansion culture tank (401). The zooplankton in the expansion culture tank (401) feeds on the algae in the algae liquid transported by the algae culture pipe (301) and proliferates rapidly. The algae density, zooplankton density and zooplankton growth status in the expansion culture tank (401) are monitored by the plankton sampling and detection mechanism, and the monitoring results are fed back to the integrated controller (500). The integrated controller (500) controls the zooplankton capture and control. The electric valve of the integrated component (402) is opened and closed, and the start and stop and flow rate of the bidirectional pump (403) are controlled to enrich the zooplankton after the expansion and cultivation reaches the standard and then release it or directly release it to a specific location of the target water body. Subsequently, the integrated controller (500) controls the water pretreatment module (100), the water quality monitoring and control module (200), the algae cultivation module (300) and the zooplankton expansion and cultivation module (400) to continuously and automatically operate, so that the algae in the algae cultivation pipeline (301) continue to proliferate and are fed into the expansion and cultivation pool (401), thereby realizing the continuous automatic expansion and external transmission of the zooplankton in the expansion and cultivation pool (401).

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