A weight-loss composition, its preparation method and applications

Abstract

The present invention provides a weight loss pharmaceutical composition, its preparation method and application. The pharmaceutical composition is composed of traditional Chinese medicines such as burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, gordon euryale seed, dried tangerine peel, etc.; its preparation method is as follows: the above-mentioned Chinese medicinal materials are steamed and boiled and then fermented with Rhizopus koji, then decocted, centrifuged, and the juice is taken out to obtain a semi-finished medicinal juice; separately, black glutinous rice is steamed and fermented with Rhizopus koji, then the boiling water and the fermented black glutinous rice are mixed, enzyme saccharification is carried out, centrifuged to take the juice, sterilized, stored in the warehouse for clarification to obtain a semi-finished black glutinous rice juice; finally, the semi-finished black glutinous rice juice and the semi-finished medicinal juice are mixed evenly, canned, sterilized, and packaged after passing the inspection of microorganisms, acidity and sugar content. This product has the effect of weight loss, with remarkable effect, no adverse reactions and toxic and side effects.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine, and specifically relates to a weight loss composition and a preparation method and application thereof. Background Art

[0002] Currently, obesity has become a global public health problem. It not only affects image and behavior, but also easily induces or aggravates high blood pressure, high blood lipids, heart disease, diabetes, fatty liver, endocrine disorders and other diseases, seriously endangering people's health. Obesity is weight gain caused by an increase in the volume of body fat and / or the number of fat cells, or an abnormal increase in the percentage of body fat to body weight, manifested as excessive fat deposition throughout the body or in certain parts of the human body.

[0003] TCM believes that obesity is a syndrome of deficiency and excess, and the main cause of the disease is the spleen and stomach. "Medical Reality Easy Volume 4" says: "The strong constitution is due to congenital... Generally speaking, the strong constitution has never suffered from anything, but there is a lot of dampness and phlegm", which shows that the body constitution is affected by congenital endowment to a certain extent. Insufficient congenital endowment may lead to spleen and kidney deficiency, and improper diet, craving for fatty and sweet food, or overeating, long-term eating more and less exercise can also lead to dysfunction of spleen and stomach. The symptoms of obesity caused by spleen deficiency and dampness are mainly manifested as obesity, potbelly, limb edema, fatigue, loss of appetite, indigestion and other symptoms. Spleen deficiency and dampness are the etiology and pathogenesis in TCM theory, which mainly refers to the retention of moisture due to spleen and stomach dysfunction, thus causing obesity symptoms.

[0004] At present, the weight loss methods are mostly adopted by taking western medicine, surgical fat reduction and dieting, etc. There are many western medicine weight loss health products on the market, but most of them have certain defects, some side effects are big such as gastrointestinal discomfort, nausea and vomiting, increased flatus, urgent defecation or incontinence, etc., some cause anorexia after taking, some will rebound, some skin loosening after weight loss, and surgical fat reduction and dieting will also cause rebound. And traditional Chinese medicine has developed compound weight loss medicines for expectoration and turbidity removal, dampness and lipid reduction, blood circulation and stasis removal, yin and blood nourishing, and qi and spleen strengthening according to the understanding of obesity mechanism, and has obvious effect. Chinese medicine has its significant effect and broad prospects in weight loss and fat removal. We should inherit and apply the traditional theory of traditional Chinese medicine on weight loss, adopt modern science and technology and methods, and conduct systematic research to screen out high-efficiency and non-toxic health weight loss prescriptions. Therefore, it is urgent to find a composition that can be safely and effectively applied to the preparation of weight loss products. Summary of the invention

[0005] The object of the present invention is to provide a weight loss composition.

[0006] Another object of the present invention is to provide a method for preparing the weight loss composition.

[0007] Another object of the present invention is to provide an application of a weight loss composition in the preparation of a weight loss product.

[0008] In order to achieve the above object, the present invention is realized by the following technical solutions:

[0009] A weight loss composition according to the present invention is prepared from the following raw materials in parts by weight: 65-85 parts of burdock root, 180-220 parts of coix seed, 80-120 parts of poria cocos, 80-120 parts of cassia seed, 80-120 parts of lotus leaf, 80-120 parts of Chinese yam, 80-120 parts of gorgon fruit, 140-160 parts of dried tangerine peel.

[0010] Further, the weight loss composition according to the present invention is prepared from the following raw materials in parts by weight: 70-80 parts of burdock root, 190-210 parts of coix seed, 90-110 parts of poria cocos, 90-110 parts of cassia seed, 90-110 parts of lotus leaf, 90-110 parts of Chinese yam, 90-110 parts of gorgon fruit, 145-155 parts of dried tangerine peel.

[0011] Still further, the weight loss composition according to the present invention is prepared from the following raw materials in parts by weight: 75 parts of burdock root, 200 parts of coix seed, 100 parts of poria cocos, 100 parts of cassia seed, 100 parts of lotus leaf, 100 parts of Chinese yam, 100 parts of gorgon fruit, 150 parts of dried tangerine peel.

[0012] An application of the weight loss composition according to the present invention in the preparation of a weight loss product.

[0013] The dosage forms of the weight loss product according to the present invention include but are not limited to: mixture, syrup, capsule, granule, tablet or pill.

[0014] A preparation method of the weight loss composition according to the present invention includes the following steps:

[0015] 1) Take burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, gorgon fruit, and dried tangerine peel according to the prescription ratio, pulverize, and steam for 0.5-1.5 h; naturally cool to 25-35 °C, add Rhizopus koji and stir evenly, ferment for 1.5-7 d, then add 4-10 times the amount of water and decoct at 100 °C for 0.5-2 h, centrifuge, and extract the juice to obtain the semi-finished medicinal juice;

[0016] 2) Take 250-300 parts of black glutinous rice, soak for 2-4 h, wash it clean, steam it in a steamer, the steaming temperature is 100-156 °C, steam for 0.5-2 h, cool to 25-35 °C, add Rhizopus koji and stir evenly, ferment, and the fermentation time is 1-7 d to obtain the fermented black glutinous rice;

[0017] 3) Take 100 - 300 parts of boiled water and mix it with fermented black glutinous rice. Add saccharifying enzyme and saccharify at a temperature of 55 - 80°C for 1 - 5 hours. Centrifuge to obtain juice, and detect the acid and sugar content. The reducing sugar is 200 g / L - 250 g / L, and the acidity is within 1 - 6 g / L. Then sterilize and inactivate the enzyme at 100°C for 5 - 30 minutes, or perform instant high-temperature sterilization. Store in the warehouse for clarification for 2 - 7 days, and take the supernatant to obtain semi-finished black glutinous rice juice;

[0018] 4) Take the semi-finished black glutinous rice juice and the semi-finished medicinal juice, mix them evenly according to a volume ratio of 1:0.1 - 1, measure the acid and sugar content. The reducing sugar is 50 g - 110 g / L, and the acidity is within 1 - 6 g / L. Can the product, sterilize it in a sterilization kettle, the sterilization temperature is 100°C, and the sterilization time is 10 - 60 minutes. After checking that the microorganisms and acid and sugar content are qualified, package it to obtain the product.

[0019] Preferably, the preparation method of the weight-loss composition of the present invention includes the following steps:

[0020] 1) Take burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, gorgon fruit, and tangerine peel according to the prescription ratio, crush them, and steam them for 1 hour; naturally cool down to 30°C, add Rhizopus koji and stir evenly, ferment for 7 days, then add 4 - 10 times the amount of water and decoct at 100°C for 1 hour, centrifuge, and obtain juice to get semi-finished medicinal juice;

[0021] 2) Take 280 parts of black glutinous rice, soak it for 3 hours, after cleaning it, steam it in a steamer, the steaming temperature is 155°C, steam for 0.5 hour, cool down to 30°C, add Rhizopus koji and stir evenly, ferment, the fermentation time is 7 days, to obtain fermented black glutinous rice;

[0022] 3) Take 300 parts of boiled water and mix it with fermented black glutinous rice. Add saccharifying enzyme and saccharify at a temperature of 60°C for 1 hour. Centrifuge to obtain juice, and detect the acid and sugar content. The reducing sugar is 200 g / L - 250 g / L, and the acidity is within 5 g / L. Then sterilize and inactivate the enzyme at 100°C for 5 - 30 minutes or perform instant high-temperature sterilization. Store in the warehouse for clarification for 2 - 3 days, take the supernatant to obtain semi-finished black glutinous rice juice;

[0023] 4) Take the semi-finished black glutinous rice juice and the semi-finished medicinal juice, mix them evenly according to a volume ratio of 1:0.1 - 1, measure the acid and sugar content. The reducing sugar is 50 g - 110 g / L, and the acidity is within 5 g / L. Can the product, sterilize it in a sterilization kettle, the sterilization temperature is 100°C, and the sterilization time is 20 - 60 minutes. After checking that the microorganisms and acid and sugar content are qualified, package it to obtain the product.

[0024] The addition amount of the Rhizopus koji described in step 1) of the present invention is 3 - 8‰ of the total weight of the crude drugs.

[0025] The addition amount of the Rhizopus koji described in step 2) of the present invention is 3 - 8‰ of the weight of the black glutinous rice.

[0026] The addition amount of glucoamylase in step 3) of the present invention is 1-5‰ of the weight of the fermented black glutinous rice.

[0027] The parts by weight described in the content of the present invention refer to international common measurement units such as g and kg.

[0028] Arctium lappa root belongs to the lung meridian and the heart meridian. It is bitter, cold, and non-toxic. It has the effects of dispersing wind-heat, detoxifying and detumescence. It is used for wind-heat cold, cough, sore throat, constipation, dizziness caused by wind-fire disturbing, tinnitus and deafness, blurred vision. Topically, it is used for red swelling and pain of head and facial wind toxin, hot-toxic toothache, gingival swelling and pain, carbuncle and malignant sore, hemorrhoids, rheumatic arthralgia, limb contracture, etc. Research shows that Arctium lappa root is rich in dietary fiber, which can promote blood circulation, clear gastrointestinal waste, facilitate defecation, reduce cholesterol in the body, reduce the accumulation of toxins and wastes in the body, so as to achieve the effect of weight loss.

[0029] Coix seed tastes sweet and light, and is cool in nature. It belongs to the spleen, stomach and lung meridians. It invigorates the spleen and promotes diuresis, removes obstruction in the meridians and stops diarrhea, clears heat and expels pus. It is used for edema, beriberi, dysuria, damp arthralgia with contracture, spleen deficiency diarrhea, lung abscess, intestinal abscess; flat wart.

[0030] Poria cocos tastes sweet and is mild in nature. It enters the heart, lung, spleen, stomach and kidney meridians. It has the effects of promoting diuresis to eliminate dampness, benefiting the spleen and stomach, protecting the kidney, calming the nerves and promoting fluid production.

[0031] Gorgon fruit tastes sweet and astringent, and is mild in nature. It belongs to the spleen and kidney meridians. It has the effects of tonifying the kidney and astringing essence, invigorating the spleen and stopping diarrhea, removing dampness and stopping leukorrhea. It is commonly used for spermatorrhea, enuresis and frequent urination, chronic diarrhea due to spleen deficiency, leukorrhea, and vaginal discharge.

[0032] Cassia seed is bitter and warm in nature. It belongs to the kidney and liver meridians. It has the effects of clearing heat and improving eyesight, moistening the intestines and promoting defecation. It can reduce the absorption of cholesterol in the intestine, prevent the deposition of cholesterol in the liver, and relieve atherosclerosis. Literature reports that oral administration can significantly prevent the increase of serum cholesterol and triglyceride in experimental hyperlipidemic rabbits, and can prevent the formation of atherosclerotic plaques on the aortic wall.

[0033] Lotus leaf is bitter and mild in nature. It clears heat and relieves summer heat, promotes the rising of clear yang, cools blood and stops bleeding. Promoting the rising of yang qi can disperse stasis and retain good blood. Modern pharmacological research shows that lotus leaf has the effects of dilating blood vessels, spasmolysis, lowering blood pressure, and lowering blood lipid.

[0034] Chinese yam tastes sweet and is mild in nature. It belongs to the spleen, lung and kidney meridians. It can invigorate the spleen, nourish the lung, strengthen the kidney and benefit essence.

[0035] Dried tangerine peel is bitter, pungent and warm in nature. It belongs to the lung and spleen meridians. It regulates qi and invigorates the spleen, dries dampness and resolves phlegm. It is used for fullness and distension in the chest and abdomen, anorexia, vomiting and diarrhea, cough with profuse phlegm.

[0036] In the formula, Poria cocos, Euryale ferox, and Coix lacryma-jobi are the monarch drugs, strengthening the spleen and tonifying the kidney, promoting diuresis and percolating dampness; Dioscorea opposita and Arctium lappa are the ministerial drugs, assisting the monarch drugs to enhance the functions of strengthening the spleen and promoting defecation; Cassia obtusifolia and Folium Nelumbinis are used as adjuvants to moisten the intestines and relieve constipation, and Pericarpium Citri Reticulatae is added to strengthen the spleen and regulate qi, alleviating the stasis of damp turbidity. The whole formula is combined to achieve the effects of strengthening the spleen and removing dampness, and moistening the intestines and relieving constipation. The whole formula has a mild nature, avoiding the disadvantages of excessive cold or heat.

[0037] Advantages of the present invention:

[0038] 1. The weight loss composition of the present invention is a new product with significant weight loss effects. Mouse experiments have proven that: (1) During the treatment process, the body weight of the mice in the PBS treatment group showed a continuous increasing trend; while both the low-dose group (Low) and the high-dose group (High) demonstrated the ability to inhibit weight gain. On the 40th day, compared with the PBS group, the body weights of the L group and the H group decreased by more than 10%, indicating that this weight loss product can effectively control the weight gain of mice;

[0039] (2) After 40 days of treatment, the blood of the mice was tested. Compared with the PBS treatment group, the LDL-C, TG, and TC indexes of the experimental group were all lower than those of the PBS group, indicating that the fat content in the mice of the treatment group was improved;

[0040] (3) The abdominal fat of the mice in the PBS group was relatively thick. Although there was also a fat layer in the drug treatment group, compared with the PBS group, the fat layer was thinner and the fat coverage area was smaller. This indicates significant weight loss effects.

[0041] 2. The product of the present invention is safe and effective without adverse reactions. Description of the Drawings

[0042] Figure 1 : Graph of changes in body weight before and after

[0043] Figure 2 : Graph of changes in weight loss indexes

[0044] Figure 3 : Graph of body shape measurement of mice in the control group (PBS) after 40 days of feeding with SYHF60 feed

[0045] Figure 4 : Graph of body shape measurement of mice in the low-dose treatment group (Low) after 40 days of feeding with SYHF60 feed + drug

[0046] Figure 5 : Graph of body shape measurement of mice in the high-dose treatment group (High) after 40 days of feeding with SYHF60 feed + drug

[0047] Figure 6 : Graph of internal fat of mice in the control group (PBS) after 40 days of feeding with SYHF60 feed

[0048] Figure 7 : After 40 days of feeding the mice in the low-dose treatment group (Low) with SYHF60 feed, the body fat map of the mice

[0049] Figure 8 : After 40 days of feeding the mice in the high-dose treatment group (Hight) with SYHF60 feed, the body fat map of the mice

[0050] Figure 9 : Fecal map of the mice during the treatment

[0051] Figure 10 : Change map of the liver and kidney function indexes of the mice before and after the treatment

[0052] Figure 11 : Change map of the main organs of the mice in each group after the treatment Specific implementation manner

[0053] Example 1 Weight loss composition

[0054] Formula: 75 g of burdock root, 200 g of coix seed, 100 g of Poria cocos, 100 g of cassia seed, 100 g of lotus leaf, 100 g of Chinese yam, 100 g of Gorgon fruit, 150 g of dried tangerine peel.

[0055] Example 2 Weight loss composition

[0056] Formula: 85 g of burdock root, 180 g of coix seed, 120 g of Poria cocos, 120 g of cassia seed, 80 g of lotus leaf, 80 g of Chinese yam, 80 g of Gorgon fruit, 140 g of dried tangerine peel.

[0057] Example 3 Weight loss composition

[0058] Formula: 65 g of burdock root, 220 g of coix seed, 80 g of Poria cocos, 80 g of cassia seed, 120 g of lotus leaf, 120 g of Chinese yam, 120 g of Gorgon fruit, 160 g of dried tangerine peel.

[0059] Example 4 Weight loss composition

[0060] Formula: 80 g of burdock root, 190 g of coix seed, 110 g of Poria cocos, 110 g of cassia seed, 90 g of lotus leaf, 110 g of Chinese yam, 90 g of Gorgon fruit, 145 g of dried tangerine peel.

[0061] Example 5 Weight loss composition

[0062] Formula: 70 g of burdock root, 210 g of coix seed, 90 g of Poria cocos, 90 g of cassia seed, 110 g of lotus leaf, 90 g of Chinese yam, 110 g of Gorgon fruit, 155 g of dried tangerine peel.

[0063] Example 6 Weight loss composition

[0064] Formula: 75 g of burdock root, 200 g of coix seed, 110 g of poria cocos, 110 g of cassia seed, 110 g of lotus leaf, 110 g of Chinese yam, 110 g of gorgon fruit, 150 g of dried tangerine peel.

[0065] Example 7 Weight Loss Composition

[0066] Formula: 80 g of burdock root, 200 g of coix seed, 120 g of poria cocos, 120 g of cassia seed, 120 g of lotus leaf, 120 g of Chinese yam, 120 g of gorgon fruit, 150 g of dried tangerine peel.

[0067] Example 8 Weight Loss Composition

[0068] Formula: 70 g of burdock root, 190 g of coix seed, 90 g of poria cocos, 90 g of cassia seed, 90 g of lotus leaf, 100 g of Chinese yam, 100 g of gorgon fruit, 150 g of dried tangerine peel.

[0069] Example 9 Weight Loss Composition

[0070] Formula: 60 g of burdock root, 150 g of coix seed, 75 g of poria cocos, 100 g of cassia seed, 70 g of lotus leaf, 75 g of Chinese yam, 75 g of gorgon fruit, 60 g of dried tangerine peel.

[0071] Example 10 Weight Loss Composition

[0072] Formula: 75 g of burdock root, 200 g of coix seed, 100 g of poria cocos, 100 g of cassia seed, 100 g of lotus leaf, 100 g of Chinese yam, 100 g of gorgon fruit, 160 g of dried tangerine peel.

[0073] Example 11 Weight Loss Composition

[0074] Formula: 80 g of burdock root, 200 g of coix seed, 120 g of poria cocos, 110 g of cassia seed, 110 g of lotus leaf, 100 g of Chinese yam, 100 g of gorgon fruit, 150 g of dried tangerine peel.

[0075] The formulas of the weight loss compositions in Examples 1-11 are used in the following preparation methods.

[0076] Preparation of the Weight Loss Composition in Example 12

[0077] (1) Take burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, gorgon fruit, and dried tangerine peel according to the prescription ratio, pulverize, and steam for 1 h; naturally cool to 30 °C, add 5‰ of Rhizopus koji based on the total amount of medicinal materials, stir evenly, ferment at 30 °C for 7 d, then add 2000 g of water and decoct at 100 °C for 1 h, centrifuge, and extract the juice to obtain the medicinal juice for standby;

[0078] (2) Take 280 g of black glutinous rice, soak it for 3 h, wash it clean, then steam it in a steamer at a steaming temperature of 155 °C for 0.5 h. After cooling it down to 30 °C, add Rhizopus koji accounting for 5‰ of the weight of the black glutinous rice, stir evenly, and ferment at 30 °C for 7 d to obtain fermented black glutinous rice;

[0079] (3) Take 300 g of boiling water and mix it with the fermented black glutinous rice. Add 3‰ saccharifying enzyme and saccharify at 60 °C for 1 h, then centrifuge to obtain juice, and detect the acid sugar content. The reducing sugar is 200 g / L - 250 g / L, and the acidity is within 5 g / L. Then sterilize and inactivate the enzyme at 100 °C for 5 min, store it in the warehouse for clarification for 3 d, and take the supernatant to obtain semi-finished black glutinous rice juice;

[0080] (4) Mix the semi-finished black glutinous rice juice and the semi-finished medicinal juice evenly according to the volume ratio of 1:0.1 - 1, detect the acid sugar content. The reducing sugar is 50 g - 110 g / L, and the acidity is within 5 g / L. Can the product, sterilize it in a sterilization kettle at a sterilization temperature of 100 °C for 20 min. After checking that the microorganisms and acid sugar content are qualified, package it to obtain the product.

[0081] Preparation of the weight loss composition in Example 13

[0082] (1) Take burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, euryale ferox, and tangerine peel according to the prescription ratio, crush them, and steam for 0.5 h; naturally cool down to 35 °C, add Rhizopus koji accounting for 3‰ of the total amount of the medicinal materials, stir evenly, ferment at 35 °C for 3 d, then add 1000 g of water and decoct at 100 °C for 0.5 h, centrifuge, and take out the juice to obtain the medicinal juice for standby;

[0083] (2) Take 250 g of black glutinous rice, soak it for 2 h, wash it clean, then steam it in a steamer at a steaming temperature of 154 °C for 0.5 h. After cooling it down to 35 °C, add Rhizopus koji accounting for 3‰ of the weight of the black glutinous rice, stir evenly, and ferment at room temperature of 35 °C for 3 d to obtain fermented black glutinous rice;

[0084] (3) Take 100 g of boiling water and mix it with the fermented black glutinous rice. Add 1‰ saccharifying enzyme and saccharify at 55 °C for 2 h, then centrifuge to obtain juice, and detect the acid sugar content. The reducing sugar is 200 g / L - 250 g / L, and the acidity is within 3 g / L. Then sterilize and inactivate the enzyme at 100 °C for 10 min, store it in the warehouse for clarification for 2 d, and take the supernatant to obtain semi-finished black glutinous rice juice;

[0085] (4) Mix the semi-finished black glutinous rice juice and the semi-finished medicinal juice evenly according to the volume ratio of 1:1, detect the acid sugar content. The reducing sugar is 50 g - 110 g / L, and the acidity is within 3 g / L. Can the product, sterilize it in a sterilization kettle at a sterilization temperature of 100 °C for 15 min. After checking that the microorganisms and acid sugar content are qualified, package it to obtain the product.

[0086] Preparation of the weight loss composition in Example 14

[0087] (1) Weigh burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, gordon euryale seed and tangerine peel according to the prescription ratio, crush them, and steam them for 1.5 h; let them cool naturally to 25 °C, add Rhizopus koji at 8‰ of the total amount of the medicinal materials, stir evenly, ferment at 25 °C for 5 d, then add 4000 g of water and decoct at 100 °C for 1.5 h, centrifuge and extract the juice to obtain the medicinal juice for standby;

[0088] (2) Take 300 g of black glutinous rice, soak it for 4 h, wash it clean, then steam it in a steamer at a steaming temperature of 155 °C for 1 h, cool it to 25 °C, add Rhizopus koji at 8‰ of the weight of the black glutinous rice, stir evenly, and ferment at room temperature of 25 °C for 5 d to obtain fermented black glutinous rice;

[0089] (3) Mix 300 g of boiling water with the fermented black glutinous rice, add 5‰ glucoamylase and saccharify at 60 °C for 4 h, centrifuge and extract the juice, detect the acid sugar content, with the reducing sugar being 200 g / L - 250 g / L and the acidity within 6 g / L; then sterilize and inactivate the enzyme at 100 °C for 30 min, store it in the warehouse for clarification for 3 d, and take the supernatant to obtain the semi-finished black glutinous rice juice;

[0090] (4) Mix the semi-finished black glutinous rice juice and the semi-finished medicinal juice evenly according to the volume ratio of 1:1, detect the acid sugar content, with the reducing sugar being 50 g - 110 g / L and the acidity within 6 g / L. Can the product, sterilize it in a sterilization kettle at a sterilization temperature of 100 °C for 30 min. After checking that the microorganisms and acid sugar content are qualified, package it to obtain the product.

[0091] Preparation of the weight loss composition in Example 15

[0092] (1) Weigh burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, gordon euryale seed and tangerine peel according to the prescription ratio, crush them, and steam them for 1 h; let them cool naturally to 30 °C, add Rhizopus koji at 5‰ of the total amount of the medicinal materials, stir evenly, ferment at 30 °C for 7 d, decoct at 100 °C for 1 h, centrifuge and extract the juice to obtain the medicinal juice for standby;

[0093] (2) Take 280 g of black glutinous rice, soak it for 3 h, wash it clean, then steam it in a steamer at a steaming temperature of 120 °C for 0.5 h, cool it to 30 °C, add Rhizopus koji at 5‰ of the weight of the black glutinous rice, stir evenly, and ferment at room temperature of 30 °C for 7 d to obtain fermented black glutinous rice;

[0094] (3) Mix 300 g of boiling water with the fermented black glutinous rice, add 3‰ glucoamylase and saccharify at 60 °C for 1 h, centrifuge and extract the juice, detect the acid sugar content, with the reducing sugar being 200 g / L - 250 g / L and the acidity within 5 g / L; then sterilize and inactivate the enzyme at 100 °C for 5 min, store it in the warehouse for clarification for 2 d, and take the supernatant to obtain the semi-finished black glutinous rice juice;

[0095] (4) Take the semi-finished black glutinous rice juice and semi-finished medicinal juice in a volume ratio of 1:1, mix them evenly, detect the acid and sugar content, with the reducing sugar being 50 g - 110 g / L and the acidity within 3 g / L. The product is canned and sterilized in a sterilization kettle at a sterilization temperature of 100 °C for 50 min. After checking that the microorganisms and acid and sugar content are qualified, it is packaged to obtain the product.

[0096] Example 16 Preparation of Weight Loss Composition

[0097] (1) Take burdock root, coix seed, tuckahoe, cassia seed, lotus leaf, Chinese yam, gorgon fruit, and dried tangerine peel according to the prescription ratio, crush them, and steam for 1 h; naturally cool to 30 °C, add 3.5‰ of Rhizopus koji based on the total amount of the medicinal materials, stir evenly, ferment for 2 d, decoct at 100 °C for 1 h, centrifuge, and extract the juice to obtain the medicinal juice for standby.

[0098] (2) Take 280 g of black glutinous rice, soak it for 3 h, wash it clean, then steam it in a steamer at a steaming temperature of 110 °C for 50 min, cool it down and spread it out to 30 °C, add 3.5‰ of Rhizopus koji based on the weight of the black glutinous rice, stir evenly, and ferment for 2 d to obtain the fermented black glutinous rice.

[0099] (3) Take 300 g of boiled water and mix it with the fermented black glutinous rice, add 1.6‰ of glucoamylase at 60 °C for saccharification for 3 h, centrifuge to extract the juice, detect the acid and sugar content, with the reducing sugar being 200 g / L - 250 g / L and the acidity within 5 g / kg; then sterilize and inactivate the enzyme at 100 °C for 10 min, store it in the warehouse for clarification for 2 d, and take the supernatant to obtain the semi-finished black glutinous rice juice.

[0100] (4) Take the semi-finished black glutinous rice juice and semi-finished medicinal juice in a volume ratio of 1:0.1 - 1, mix them evenly, detect the acid and sugar content, with the reducing sugar being 50 g - 110 g / L and the acidity within 5 g / L. The product is canned and sterilized in a sterilization kettle at a sterilization temperature of 100 °C for 50 min. After checking that the microorganisms and acid and sugar content are qualified, it is packaged to obtain the product.

[0101] Example 17 Preparation of Weight Loss Composition

[0102] (1) Take burdock root, coix seed, tuckahoe, cassia seed, lotus leaf, Chinese yam, gorgon fruit, and dried tangerine peel according to the prescription ratio, crush them, and steam for 1 h; naturally cool to 25 °C, add 4‰ of Rhizopus koji based on the total amount of the medicinal materials, stir evenly, ferment for 2 d, decoct at 100 °C for 1 h, centrifuge, and extract the juice to obtain the medicinal juice for standby.

[0103] (2) Take 280 g of black glutinous rice, soak it for 3 h, wash it clean, then steam it in a steamer at a steaming temperature of 110 °C for 50 min, cool it down and spread it out to 25 °C, add 4‰ of Rhizopus koji based on the weight of the black glutinous rice, stir evenly, and ferment for 1.5 d to obtain the fermented black glutinous rice.

[0104] (3) Take 300 g of boiled water and mix it with fermented black glutinous rice. Add 1.6‰ glucoamylase and saccharify at 60°C for 2 h. Centrifuge to obtain the juice, and detect the acid sugar content. The reducing sugar is 200 g / L - 250 g / L, and the acidity is within 5 g / L. Then, sterilize and inactivate the enzyme at 100°C for 10 min, store in the warehouse for clarification for 2 d, and take the supernatant to obtain semi-finished black glutinous rice juice;

[0105] (4) Mix the semi-finished black glutinous rice juice and the semi-finished medicinal juice evenly according to a volume ratio of 1:0.1 - 1. Detect the acid sugar content. The reducing sugar is 50 g - 110 g / L, and the acidity is within 5 g / L. Can the product, sterilize it in a sterilization kettle, the sterilization temperature is 100°C, and the sterilization time is 50 min. After checking that the microorganisms and acid sugar content are qualified, package it to obtain the product.

[0106] Preparation of the weight loss composition in Example 18

[0107] (1) Take burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, euryale ferox, and tangerine peel according to the prescription ratio, crush them, and steam for 1 h. Naturally cool down to 30°C, add 5‰ Rhizopus koji based on the total amount of the medicinal materials, stir evenly, ferment for 3 d, decoct at 100°C for 1 h, centrifuge, and extract the juice to obtain the medicinal juice for standby;

[0108] (2) Take 280 g of black glutinous rice, soak it for 3 h, wash it clean, then steam it in a steamer at a temperature of 110°C for 30 min. Cool it down and spread it out to 30°C, add 5‰ Rhizopus koji based on the weight of the black glutinous rice, stir evenly, and ferment for 3 d to obtain fermented black glutinous rice;

[0109] (3) Take 300 g of boiled water and mix it with fermented black glutinous rice. Add 2.6‰ glucoamylase and saccharify at 60°C for 3 h. Centrifuge to obtain the juice, and detect the acid sugar content. The reducing sugar is 200 g / L - 250 g / L, and the acidity is within 5 g / L. Then, sterilize and inactivate the enzyme at 100°C for 10 min, store in the warehouse for clarification for 2 d, and take the supernatant to obtain semi-finished black glutinous rice juice;

[0110] (4) Mix the semi-finished black glutinous rice juice and the semi-finished medicinal juice evenly according to a volume ratio of 1:0.1 - 1. Detect the acid sugar content. The reducing sugar is 50 g - 110 g / L, and the acidity is within 5 g / L. Can the product, sterilize it in a sterilization kettle, the sterilization temperature is 100°C, and the sterilization time is 40 min. After checking that the microorganisms and acid sugar content are qualified, package it to obtain the product.

[0111] Preparation of the weight loss composition in Example 19

[0112] (1) Take burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, euryale ferox, and tangerine peel according to the prescription ratio, crush them, and steam for 1 h. Naturally cool down to 30°C, add 5.5‰ Rhizopus koji based on the total amount of the medicinal materials, stir evenly, ferment for 4 d, decoct at 100°C for 1 h, centrifuge, and extract the juice to obtain the medicinal juice for standby;

[0113] (2) Take 280 g of black glutinous rice, soak it for 4 h, wash it clean, then steam it in a steamer at a temperature of 120 °C for 50 min, cool it down to 30 °C, add Rhizopus koji at 5.5‰ of the weight of black glutinous rice, stir evenly, and ferment for 2 d to obtain fermented black glutinous rice;

[0114] (3) Take 300 g of boiled water and mix it with the fermented black glutinous rice, add 2.6‰ glucoamylase at a temperature of 60 °C and saccharify for 2 h, centrifuge to obtain juice, and detect the acid sugar content. The reducing sugar is 200 g / L - 250 g / L, and the acidity is within 5 g / L; then sterilize and inactivate the enzyme at 100 °C for 10 min, store it in the warehouse for clarification for 2 d, and take the supernatant to obtain semi-finished black glutinous rice juice;

[0115] (4) Mix the semi-finished black glutinous rice juice and the semi-finished medicinal juice evenly according to the volume ratio of 1:0.1 - 1, detect the acid sugar content, the reducing sugar is 50 g - 110 g / L, and the acidity is within 5 g / L. The product is canned, sterilized in a sterilization kettle at a temperature of 100 °C for 50 min. After checking that the microorganisms and acid sugar content are qualified, it is packaged to obtain the product.

[0116] Preparation of the weight loss composition in Example 20

[0117] (1) Take burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, euryale ferox, and tangerine peel according to the prescription ratio, crush them, and steam for 1 h; naturally cool down to 35 °C, add Rhizopus koji at 4.5‰ of the total amount of medicinal materials, stir evenly, ferment for 4 d, decoct at 100 °C for 1 h, centrifuge, and extract the juice to obtain the medicinal juice for standby;

[0118] (2) Take 280 g of black glutinous rice, soak it for 2 h, wash it clean, then steam it in a steamer at a temperature of 110 °C for 50 min, cool it down to 35 °C, add Rhizopus koji at 4.5‰ of the weight of black glutinous rice, stir evenly, and ferment for 4 d to obtain fermented black glutinous rice;

[0119] (3) Take 300 g of boiled water and mix it with the fermented black glutinous rice, add 3.2‰ glucoamylase at a temperature of 60 °C and saccharify for 2 h, centrifuge to obtain juice, and detect the acid sugar content. The reducing sugar is 200 g / L - 250 g / L, and the acidity is within 5 g / L; then sterilize and inactivate the enzyme at 100 °C for 10 min, store it in the warehouse for clarification for 2 d, and take the supernatant to obtain semi-finished black glutinous rice juice;

[0120] (4) Mix the semi-finished black glutinous rice juice and the semi-finished medicinal juice evenly according to the volume ratio of 1:0.1 - 1, detect the acid sugar content, the reducing sugar is 50 g - 110 g / L, and the acidity is within 5 g / L. The product is canned, sterilized at a temperature of 100 °C for 30 min. After checking that the microorganisms and acid sugar content are qualified, it is packaged to obtain the product.

[0121] Preparation of the weight loss composition in Example 21

[0122] (1) Weigh burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, gordon euryale seed, and dried tangerine peel according to the prescription ratio, crush them, and steam for 1 hour; let it cool naturally to 40°C, add Rhizopus koji at 5‰ of the total weight of the medicinal materials, stir evenly, ferment for 5 days, decoct at 100°C for 1 hour, centrifuge, and extract the juice to obtain the medicinal juice for standby;

[0123] (2) Take 280 g of black glutinous rice, soak it for 3 hours, wash it clean, then steam it in a steamer at a temperature of 130°C for 40 minutes, cool it to 40°C, add Rhizopus koji at 5‰ of the weight of the black glutinous rice, stir evenly, and ferment for 5 days to obtain fermented black glutinous rice;

[0124] (3) Mix 300 g of boiling water with the fermented black glutinous rice, add 3.5‰ glucoamylase at a temperature of 60°C for saccharification for 1 hour, centrifuge to extract the juice, detect the acid sugar content, the reducing sugar is 200 g / L - 250 g / L, and the acidity is within 5 g / kg; then sterilize and inactivate the enzyme at 100°C for 10 minutes, store it in the warehouse for clarification for 2 days, and take the supernatant to obtain the semi-finished black glutinous rice juice;

[0125] (4) Mix the semi-finished black glutinous rice juice and the semi-finished medicinal juice evenly according to the volume ratio of 1:0.1 - 1, detect the acid sugar content, the reducing sugar is 50 g - 110 g / L, and the acidity is within 5 g / L. The product is canned and sterilized in a sterilization kettle at a temperature of 100°C for 50 minutes. After checking that the microorganisms and acid sugar content are qualified, it is packaged to obtain the product.

[0126] Example 22 Preparation of Weight Loss Composition

[0127] (1) Weigh burdock root, coix seed, poria cocos, cassia seed, lotus leaf, Chinese yam, gordon euryale seed, and dried tangerine peel according to the prescription ratio, crush them, and steam for 1 hour; let it cool naturally to 40°C, add Rhizopus koji at 5‰ of the total weight of the medicinal materials, stir evenly, ferment for 7 days, then add 2000 g of water and decoct at 100°C for 1 hour, centrifuge, and extract the juice to obtain the medicinal juice for standby;

[0128] (2) Take 280 g of black glutinous rice, soak it for 3 hours, wash it clean, then steam it in a steamer at a temperature of 155°C for 0.5 hour, cool it to 35°C, add Rhizopus koji at 5‰ of the weight of the black glutinous rice, stir evenly, and ferment for 7 days to obtain fermented black glutinous rice;

[0129] (3) Mix 300 g of boiling water with the fermented black glutinous rice, add 3‰ glucoamylase at a temperature of 60°C for saccharification for 2 hours, centrifuge to extract the juice, detect the acid sugar content, the reducing sugar is 200 g / L - 250 g / L, and the acidity is within 5 g / L; then sterilize at high temperature instantly, store it in the warehouse for clarification for 3 days, and take the supernatant to obtain the semi-finished black glutinous rice juice;

[0130] (4) taking the semi-finished black glutinous rice juice and the semi-finished medicinal juice in a volume ratio of 1:0.1, mixing them evenly, testing the acidity and sugar content, reducing sugar 50g-110g / L, acidity within 3g / L, canning the product, sterilizing in a retort, sterilizing at a temperature of 100°C for 50min, and packaging after checking the microorganisms and acidity and sugar content.

[0131] In order to further verify the effectiveness of the present invention, the inventor conducted a series of verification experiments, the excerpts of which are as follows:

[0132] 1 Experimental instruments, materials and reagents

[0133] 1.1 Instrument

[0134] Centrifuge (LS450, Zhangjiagang Wuzhong Machinery Manufacturing Co., Ltd.), ultra-high temperature instantaneous sterilizer (RP6L10, Xiangtan Xiangwei Food Machinery Co., Ltd.), third-generation oatmeal cooking pot (JYD-ZLF-500Z, Jiangxi Jingye Machinery Technology Co., Ltd.), stirring tank (TW-TP-2000, Jiangxi Tianwo Machinery Technology Co., Ltd.), sterilizer (R2006-360, Zhucheng Zhongtai Machinery Co., Ltd.), pH meter (PHS-25, Leici), clean bench (VD-650, Tianjin Chengtai Instrument Factory), burette, sealed tank, etc.

[0135] 1.2 Raw materials

[0136] Black glutinous rice (purchased from Hongying Agricultural Machinery Farmers’ Professional Cooperative in Huishui County), Rhizopus koji, and saccharifying enzyme.

[0137] 1.3 Reagents

[0138] Plate count agar (PCA), crystal violet neutral red bile agar (VRBA), glucose, sodium chloride and other analytical grade.

[0139] 2 Preparation process

[0140] 2.1 Medicinal material processing

[0141] Recipe: 75g burdock root, 200g coix seed, 100g poria, 100g cassia seed, 100g lotus leaf, 100g yam, 100g Euryale ferox, and 150g tangerine peel.

[0142] Method 1: Grind the herbs → steam for 20 minutes → cool naturally to 30℃ → add Rhizopus koji and stir evenly → ferment at 30℃ for 72 hours → add 4-10 times (1000-4000g) water to the fermented herbs and boil at 100℃ for 60 minutes → centrifuge;

[0143] Method 2: Grind the medicinal materials → Steam for 60 minutes → Naturally cool down to 30°C → Add Rhizopus koji and stir evenly → Ferment at 30°C for 72 hours → Add 4 - 10 times the amount (1000 - 4000 g) of water to the fermented medicine and boil at 100°C for 60 minutes → Centrifuge;

[0144] Method 3: Grind the medicinal materials → Steam for 90 minutes → Naturally cool down to 30°C → Add Rhizopus koji and stir evenly → Ferment at 30°C for 72 hours → Add 4 - 10 times the amount (1000 - 4000 g) of water to the fermented medicine and boil at 100°C for 60 minutes → Centrifuge.

[0145] Results: When steaming for 20 minutes, the growth of fermenting microorganisms is slow; when steaming for 60 minutes, the growth of fermenting microorganisms is vigorous; when steaming for 90 minutes, the growth of fermenting microorganisms is slow. For the post-treatment, when steaming for 20 minutes, the taste of the fermented medicinal materials is light; when steaming for 60 minutes, the taste of the fermented medicinal materials is strong; when steaming for 90 minutes, the taste of the fermented medicinal materials is bitter.

[0146] 2.2 Treatment of black glutinous rice

[0147] 2.2.1 Fermentation

[0148] 280 kg of black glutinous rice → Soak for 3 hours → Wash → Steamer parameters: steaming temperature 100 - 156°C, steam steaming time 15 - 50 minutes (drain the water below) → Cool down and spread out to 30°C → Add Rhizopus koji and stir evenly, fermentation time 72 hours.

[0149] 2.2.1.1 Investigation of soaking time

[0150] According to the above method for treating black glutinous rice, soak it for 1 h, 3 h, 6 h, and 9 h respectively, and investigate the effects of different soaking times on the appearance, taste, and sensory properties of the product.

[0151] Results: There are differences between soaking for 9 hours and 3 hours. When soaking for 9 hours, a large amount of anthocyanins is lost, the rice is overcooked and clumped, the cooling is not thorough, the space between materials is small, the Rhizopus koji fermentation is not thorough, the juice yield is low, and the juice is turbid and not clear, with a poor taste and a bad product color, affecting the taste and sensory properties of the product. There are differences between soaking for 1 hour and 3 hours. When soaking for 1 hour, the black glutinous rice is not easily cooked through, has a raw taste, and the fermentation of the Rhizopus koji in the materials is not conducive to the growth of microorganisms, the fermentation is not thorough, and the juice yield is low, with a poor taste. There are differences between soaking for 6 hours and 3 hours. When soaking for 6 hours, a large amount of nutrients such as anthocyanins is lost, it is easily cooked and clumped during steaming, it is not easy to cool down, the fermentation of the Rhizopus koji between materials is not thorough, affecting the growth of microorganisms, the juice yield is less, and the taste is not good. The best soaking time is 3 hours. The fermentation of the Rhizopus koji in the materials is the most thorough and sufficient, the maximum amount of nutrients is retained, the juice yield is also the largest, the color of the juice is clear, and the taste is the best.

[0152] 2.2.2 Saccharification

[0153] About 300 kg of boiled water is mixed with fermented black glutinous rice → the temperature is 60 degrees → enzymatic saccharification is carried out for 1 hour → centrifugation to obtain juice → the reducing sugar is 200 g / L - 250 g / L, and the acidity is within 5 g / L; → high-temperature sterilization and enzyme inactivation at 100 degrees for 10 minutes (or instant high-temperature sterilization) → storage for clarification for 2 - 3 days.

[0154] 2.2.2.1 Investigation of saccharification temperature

[0155] According to the above method for treating black glutinous rice, saccharification treatments are carried out at 45 degrees, 50 degrees, 60 degrees, and 70 degrees respectively, and the results at different temperatures are investigated.

[0156] Results: For saccharification at 45 degrees, the temperature is too low, and the effect is not obvious. The clarity is insufficient, and the sugar degree conversion is low. The taste is light; for saccharification at 50 degrees, the temperature is relatively low, and the effect is not obvious. The clarity is insufficient, and the sugar degree conversion is relatively low. The taste is relatively light; for saccharification at 60 degrees, the temperature is suitable, the effect is obvious, the clarity is high, the sugar degree conversion is high, and the taste is fragrant; for saccharification at 70 degrees, the temperature is relatively high, the effect is not obvious, the clarity is not high, the sugar degree conversion is relatively low, and the taste is light.

[0157] 2.2.3 Mixing treatment, sterilization, and canning

[0158] Take the supernatant of the semi-finished black glutinous rice juice and the semi-finished medicinal juice obtained above, mix them evenly according to the volume ratio of 1:0.1 - 1 → the reducing sugar is 50 - 110 g / L, and the acidity is within 5 g / L → can the product → sterilize in the sterilization kettle at 100 degrees for 20 - 60 minutes → check microorganisms, acid sugar degree.

[0159] 3 Animal experiments

[0160] 3.1 Experimental materials

[0161] 3.1.1 Experimental animals

[0162] SPF-level Kunming mice, 5-week-old females, purchased from Jiangsu Ailing Biology.

[0163] 3.1.2 Drugs, reagents, or feeds

[0164] Drug: The fermentation broth of Liai Company;

[0165] Feed: High-fat and high-sugar feed (SYHF60, lipid content 60%) purchased from Jiangsu Ailing Biology.

[0166] 3.2 Experimental methods

[0167] 3.2.1 Model establishment - Establishing rat obesity (high-fat model)

[0168] The purchased mice are randomly divided into 3 groups, with 13 mice in each group. Each group is fed with SYHF60. The body weight change is detected daily. When the body weight reaches 42 g, it can be considered that the mice have become obese.

[0169] 3.2.2 Animal grouping

[0170] Blank control group: PBS; Treatment group: Low-dose group: 0.3 ml / animal; High-dose feeding group: 0.8 ml / animal.

[0171] 3.2.3 Weight loss experiment procedures

[0172] Continue to feed with SYHF60. The experimental group administered drugs to mice by gavage at 9:00 and 16:00 every day for 40 consecutive days, and record the changes in the body weight of mice.

[0173] 3.2.4 Evaluation indicators:

[0174] Blood lipid detection indicators: TC total cholesterol; TG triglyceride; LDL-C low-density lipid.

[0175] Safety evaluation indicators: AST aspartate aminotransferase; ALT alanine aminotransferase; BUN blood urea nitrogen; CRE creatinine.

[0176] 3.2.5 Statistical method:

[0177] Use graphpad8.0 for statistical analysis.

[0178] 3.2.6 Hematoxylin and eosin staining experiment:

[0179] Experimental method:

[0180] 1) Paraffin dissolution: Place the sections in a 70 °C in situ hybridization instrument and bake the sections for 30 min.

[0181] 2) Deparaffinization: Quickly put the baked sections into xylene twice, 5 min each time.

[0182] 3) Gradient hydration: Place in 100%, 90%, 80%, 70% ethanol for 5 min each, and in distilled water for 5 min.

[0183] 4) Nuclear staining: Blot dry the water, add hematoxylin staining solution, stain for 5 min, rinse off the hematoxylin staining solution with running water, and observe the staining degree under a microscope.

[0184] 5) Differentiation: Differentiate with 1% hydrochloric acid alcohol for 1 - 3 s, wash with water, soak in tap water for 5 - 10 min to turn blue; observe the color under a microscope. If the color is not deep enough, directly add hematoxylin staining solution and stain for 1 min, then differentiate immediately and wash with water; if it is too deep, appropriately increase the differentiation time.

[0185] 6) Counterstaining: Add 1 drop of 0.5% eosin staining solution, stain for 10 - 15 s, wash with distilled water and observe under a microscope.

[0186] 7) Gradient dehydration: Dehydrate with 80%, 90%, 95% and 100% ethanol.

[0187] 8) Clearing: Xylene twice, 5 minutes each time, air dry at room temperature.

[0188] 9) Mounting: Drop a drop of neutral balsam on the tissue and cover with a cover slip. Observe and take pictures under the microscope after 24 hours.

[0189] 3.3 Experimental results

[0190] 3.3.1 Changes in body weight before and after

[0191] The changes in body weight are shown in Tables 1 to 3.

[0192] Table 1 Changes in body weight of mice in the blank control group (PBS)

[0193]

[0194]

[0195] Table 2 Changes in body weight of mice in the low-dose group (Low)

[0196]

[0197] Table 3 Changes in body weight of mice in the high-dose group (Hight)

[0198]

[0199] The results are shown in Tables 1 - 2, Figure 1 , during the treatment process, the body weight of the mice in the PBS treatment group showed a continuous increasing trend; while both the low-dose group (Low) and the high-dose group (Hight) showed the ability to inhibit body weight gain. On the 40th day, compared with the PBS group, the body weights of the L group and the H group both decreased, and the body mass of the H group decreased by more than 10%, indicating that the fermentation broth of Liai Company can effectively control the growth of mice body weight.

[0200] 3.3.2 Changes in weight loss indicators

[0201] As Figure 2 shown, after 40 days of treatment, the blood of the mice was taken for detection. Compared with the PBS treatment group, the LDL-C, TG, and TC indicators of the experimental group were all lower than those of the PBS group, indicating that the fat content in the mice of the treatment group was improved.

[0202] 3.3.3 Detection of body fat in mice

[0203] The body size and fat weight of the mice are shown in Table 4.

[0204] Table 4 Body size and fat weight of mice

[0205] Group Length (cm) Width (cm) Fat mass Statistics PBS 11.5±0.4 5±0.8 1.42±0.46 / Low 11.3±0.63 4.67±0.53 0.79±0.58 *p<0.05 Hight 11.1±0.31 3.9±0.63 0.57±0.49 *p<0.05

[0206] As Figures 3 to 8 shown, the abdominal fat of the mice in the PBS group was thicker. Although there was also a fat layer in the abdominal part of the drug treatment group, compared with the PBS group, the fat layer was thinner and the fat coverage area was smaller.

[0207] 3.4 Safety evaluation

[0208] The measured values of the safety evaluation are shown in Table 5.

[0209] Table 5 Measured values of safety evaluation

[0210]

[0211] As can be seen from Table 5, compared with the control group experiment, the safety evaluation indicators AST, ALT, BUN, and CRE after using the weight loss product of the present invention have been effectively improved and are approaching the normal range values.

[0212] As Figure 9 shown, from the feces of the mice, the feces were all granular, indicating that during the treatment, the mice did not show adverse reactions such as diarrhea.

[0213] As Figure 10 , the liver and kidney functions of the mice before and after treatment were good and were all within the normal range. In addition, it can also be seen through hematoxylin and eosin staining that, as Figure 11 shown, there were no significant pathological damages in the main organs of the mice after treatment, indicating that the Li'ai weight loss fermentation broth is safe.

[0214] 4 Clinical trials

[0215] The product obtained by preparing the formula of the embodiment according to the preparation method of Example 10 of the present invention was used in clinical trials.

[0216] The participating subjects were randomly divided into two groups, namely the control group and the experimental group, with 40 people in each group and the male-female ratio of 1:1. Among them, the experimental group took one product 1 hour after lunch and dinner every day; the weight of the test population was measured at 10:00 and 15:00 every day, and the results were statistically analyzed as the average of the two measured values per day. 10 ml of blood from the test population was collected using a blood collection tube for testing. The test period was 30 days. After the test was completed, blood samples from the test population were collected for detection and analysis. The urine and feces of the test population were collected for testing. Specifically as follows:

[0217] 4.1 Basic information of the test population, see Table 6.

[0218] Table 6 Basic information of the test population

[0219] Item Weight loss product group Control group Number of cases 40 40 Male / Female 20 / 20 20 / 20 Age (years) 40.5±8 40.7±10 Course of disease (years) / / Weight (kg) 75.9±3.7 74.6±4.3 Body mass index (%) 25.7±0.6 26.4±0.8 Fat percentage (%) 27.5±0.4 26.7±0.6

[0220] As can be seen from Table 6, there should be no significant differences in the data of each index (P>0.05), and they are comparable. The measured data will be reliable and close to the true data.

[0221] 4.2 Weight changes before and after (X±SD)

[0222] The weight changes before and after are shown in Table 7.

[0223] Table 7 Weight changes before and after of the test population

[0224] Grouping Pre-meal weight (kg) Post-meal weight (kg) Weight loss product group 75.9±3.7 72.3±4.8 Control group 74.6±4.3 75.5±3.1

[0225] As can be seen from Table 7, the weight difference between before and after taking the weight loss product in the weight loss product group is about 3.6 kg under 30 days of treatment, and the average weight loss is significant; there is a significant difference between the weight loss product group after taking and the control group, which fully shows that the tested weight loss product has the effect of reducing weight.

[0226] 4.3 Changes in fat detection indexes before and after (X±SD)

[0227] The changes in fat detection indexes before and after are shown in Table 8.

[0228] Table 8 Changes in fat detection indexes before and after

[0229]

[0230] As can be seen from Table 8, the test results of the indexes of the subjects before and after the test through fat detection all decreased significantly and returned to or approached the normal values (LDL-C<2.6; TG<1.7; TC<200).

[0231] 4.4 Changes in hematological detection indexes before and after (X±SD)

[0232] The changes in hematological detection indexes before and after are shown in Table 9.

[0233] Table 9 Changes in hematological detection indexes before and after

[0234]

[0235] As can be seen from Table 9, the test results of the subjects before and after the test through blood routine, blood biochemical indexes, and routine urine and stool tests are all within the normal range.

[0236] Although the present invention has been described in detail above with general descriptions, specific embodiments and tests, some modifications or improvements can be made on the basis of the present invention, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. A composition for weight loss, characterized in that: The invention is prepared from the following raw materials in parts by weight: 75 parts of burdock root, 200 parts of coix seed, 100 parts of tuckahoe, 100 parts of cassia seed, 100 parts of lotus leaf, 100 parts of yam, 100 parts of euryale ferox, and 150 parts of tangerine peel. The preparation method comprises the following steps: 1) Take burdock root, coix seed, poria, cassia seed, lotus leaf, yam, Euryale ferox, tangerine peel according to the prescription ratio, crush, and steam for 1 hour; naturally cool to 30℃, add 3-8‰ of the total weight of the medicinal materials, stir evenly, ferment for 7 days, then add 4-10 times water and boil at 100℃ for 1 hour, centrifuge, extract the juice, and obtain the semi-finished medicinal juice; 2) Take 280 portions of black glutinous rice, soak for 3 h, clean, steam in a steamer at 155 °C for 0.5 h, cool to 30 °C, add 3-8‰ of black glutinous rice weight of Rhizopus koji, stir evenly, ferment for 7 days, and obtain fermented black glutinous rice; 3) Mix 300 portions of boiled water with the fermented black glutinous rice, add 1-5‰ of the weight of the black glutinous rice with saccharifying enzyme at 60°C for 1 hour, centrifuge to extract juice, test the acid-sugar content, the reducing sugar is 200g / L-250g / L, and the acidity is within 5g / L; then sterilize at 100°C for 5min-20min or sterilize at high temperature for an instant, put into storage for clarification for 2-3 days, take the supernatant, and obtain the semi-finished black glutinous rice juice; 4) Take the semi-finished black glutinous rice juice and the semi-finished medicinal juice, mix them evenly in a volume ratio of 1:0.1-1, test the acid-sugar content, the reducing sugar is 50g-110g / L, the acidity is within 5g / L, the product is canned, sterilized in a retort, the sterilization temperature is 100℃, the sterilization time is 20-60min, and the microorganisms and acid-sugar content are checked and packaged after they are qualified.

2. Use of the weight loss composition as claimed in claim 1 in preparing a weight loss product.

3. The use according to claim 2, characterized in that: The dosage form of the weight loss product includes, but is not limited to, mixture, syrup, capsule, granule, tablet or pill.

Images