Skin care composition with keratinization envelope repairing efficacy and use thereof

Abstract

The application discloses a skin care composition with keratinization envelope repairing effect and application thereof. The skin care composition is prepared by compounding Dendrobium candidum stem extract and / or Glycyrrhiza inflata Bat. extract on the basis of hyaluronic acid lysine copper chelate, and the skin care composition can promote filaggrin (FLG), involucrin (IVL), loricrin (LOR), keratin (KRT1) and keratinocyte transglutaminase (TGM1) protein expression, so that the effect of repairing keratinization envelope is achieved, and the skin care composition can be used for improving skin barrier and redness and other problems in cosmetics.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a skin care composition with keratinization envelope repair effect and application thereof. BACKGROUND

[0002] The periphery of the keratinocyte is wrapped by the keratinization envelope, and the intercellular lipid such as ceramide is surrounded outside the keratinocyte, which is combined with the keratinization envelope through covalent bond, endowing the keratinization envelope with the property of insolubility in water, and making the keratinocyte adhere to the intercellular lipid tightly, further improving the stability of the stratum corneum; and the keratinization envelope can also provide the correct binding site for the intercellular lipid, if the keratinization envelope is immature, even if the amount of intercellular lipid is sufficient, the neat stratum corneum brick wall structure cannot be formed; at the same time, the water insolubility of the keratinization envelope avoids the cytoplasm of the keratinocyte, such as natural moisturizing factor, from being washed out by external water, and the healthier and more complete keratinization envelope makes the keratinocyte stronger. Therefore, the keratinization envelope is the key way for the stratum corneum barrier to implement its function, and is also the basis of the epidermal mechanical defense barrier.

[0003] In the stratum corneum, keratinocytes are flattened and enucleated, which are called keratinocytes, at the same time, the cell membrane of the keratinocyte is replaced by a special barrier structure called keratinization envelope (CE). The assembly of CE starts from the upper layer of the stratum spinosum, which is mainly composed of filaggrin (FLG), involucrin (IVL), loricrin (LOR), keratin (KRT) and late cornified envelope protein (LCEs), etc. These proteins are widely cross-linked and are regulated by keratinocyte transglutaminase (TGM1). IVL is mainly expressed in the upper layer of the stratum spinosum and the stratum granulosum, which is a marker protein of keratinocyte differentiation, and is located in the outer layer of CE, which is covalently combined with ceramide containing -OH, thereby connecting the lipid matrix and keratinocyte to play a role. LOR is mainly expressed in the stratum granulosum and the stratum corneum, which is the main component of the finally differentiated CE, accounting for about 80% of the protein amount, and plays a key role in reinforcement. FLG is also a key component in the assembly process of CE, which mainly exists in the epidermal granular layer and the transparent layer. FLG participates in the aggregation of KRT1 and KRT10 filaments into high molecular weight parallel structures, thereby promoting the combination of KRT1 and KRT10 into the keratin envelope to form the flat scaffold structure of the outermost keratinocyte. TGM1 is only distributed in the middle and upper part of the stratum spinosum and the beginning of the stratum corneum in normal epidermis, which catalyzes the keratin envelope protein under the participation of FLG, and constitutes the specific stratum corneum barrier structure of the epidermis.

[0004] Hyaluronic acid lysine copper chelate has high moisturizing performance and better skin adhesion, has significant inhibitory effect on 5 alpha-reductase, can effectively control oil, prevent and treat acne vulgaris, improve skin water and oil balance, prevent seborrheic alopecia and improve hair quality, repair skin and mucosa damage, promote the generation of elastin and collagen, prevent and reduce skin wrinkles, make the skin smooth and elastic, play the effect of anti-wrinkle and firming, and improve facial aging. The preparation method of hyaluronic acid lysine copper chelate is disclosed in CN202410478415.3, specifically: after hyaluronic acid sodium and hydrochloric acid lysine copper chelate are mixed and uniformly dissolved, a proper amount of ethanol is added for precipitation and purification, filtration, dehydration with anhydrous ethanol, and drying under reduced pressure to obtain blue granular powder, which is hyaluronic acid lysine copper chelate. On this basis, the applicant develops new functions of hyaluronic acid lysine copper chelate in order to obtain more applications. SUMMARY

[0005] In view of the above problems, the present application provides a skin care composition with keratinization envelope repair effect and its application. The present application complexed iron Dendrobium stem extract and / or Glycyrrhiza inflate extract on the basis of hyaluronic acid lysine copper chelate. The test proves that it has good effect of repairing keratinization envelope and can be used in cosmetics to improve skin barrier and redness problems.

[0006] The technical scheme of the present application is: a skin care composition with keratinization envelope repair effect, characterized in that it comprises the following components by mass: 0.05-1.2 parts of hyaluronic acid lysine copper chelate, 1.0-30.0 parts of iron Dendrobium stem extract and / or Glycyrrhiza inflate extract.

[0007] The preferred ratio is: 0.05-0.5 parts of hyaluronic acid lysine copper chelate, 1.0-10.0 parts of iron Dendrobium stem extract, and 1.0-10.0 parts of Glycyrrhiza inflate extract.

[0008] The hyaluronic acid lysine copper chelate used in the present application can be commercially available or obtained by salt formation method (application number: CN202410478415.3).

[0009] The iron Dendrobium stem extract can be obtained by commercial or self-made method, and the preferred preparation method is: fresh iron Dendrobium stem is placed in a flash extractor, distilled water is added for extraction, the solid-liquid ratio is 1:15-1:25, extraction is carried out at room temperature for 2-4 times, the time is 80-110s / time, the extraction liquid is combined, concentrated, the concentrate is added with 1-2 times the mass of butanediol, stirred uniformly, and then filtered, and the obtained solution is the iron Dendrobium stem extract, that is, 6-10g of iron Dendrobium stem extract is obtained from 1g of fresh iron Dendrobium stem.

[0010] The *Glycyrrhiza uralensis* extract can be obtained commercially or in-house. A preferred preparation method is as follows: Dry *Glycyrrhiza uralensis* is pulverized, added to a 60%–80% ethanol solution, and extracted under reflux at 70–80°C 2–4 times. The filtrates are combined. The filtrate is concentrated under reduced pressure and evaporated until no obvious ethanol odor is detected. Butylene glycol is added at 1.5–2.0 times its weight of the concentrate, stirred evenly, and filtered to obtain the *Glycyrrhiza uralensis* extract. The concentration, based on the crude drug, is 0.25–0.40 g / mL, meaning 0.25–0.40 g of dried *Glycyrrhiza uralensis* powder yields 1 mL of *Glycyrrhiza uralensis* extract (solution).

[0011] Preparation method: Hyaluronic acid lysine copper chelate, Dendrobium officinale stem extract and / or Glycyrrhiza uralensis extract are mixed together and dissolved in purified water (to make up to 100 parts).

[0012] This invention combines Dendrobium officinale stem extract and / or Glycyrrhiza uralensis extract with a hyaluronic acid-lysine-copper chelate. The main active components of Dendrobium officinale are polysaccharides and amino acids, which have effects such as enhancing immunity, anti-tumor activity, lowering blood lipids, lowering blood sugar, anti-oxidation, and anti-aging. Glycyrrhiza uralensis has been isolated from licorice both domestically and internationally for over 100 flavonoids, over 60 triterpenoids, as well as coumarins, alkaloids, and organic acids, exhibiting various biological activities including antioxidant, anti-inflammatory, antibacterial, antitumor, and immunomodulatory effects, and has wide applications in the food, pharmaceutical, and cosmetic industries. Experiments have shown that the compound composition has excellent repairing effects on the keratinized membrane and can be used in cosmetics to improve the skin barrier and address redness.

[0013] This invention also provides the application of the above-described composition in the preparation of cosmetics with skin-care effects of repairing keratinized capsules. Through experimental verification, this invention demonstrates that the above-described composition can effectively repair keratinized capsules and protect the skin barrier by promoting the expression of proteins related to keratinized capsule formation.

[0014] The present invention also provides a cosmetic with skin care effect of repairing keratinized capsule, characterized in that the cosmetic contains 0.5 to 30% (preferably 2 to 20%) of a skin care composition with skin care effect of repairing keratinized capsule.

[0015] The aforementioned cosmetics for repairing and protecting the skin from keratosis pilaris may also contain emulsifiers, emollients, moisturizers, thickeners, preservatives, pH adjusters, and other ingredients permitted in the cosmetics industry.

[0016] Furthermore, by rationally adding the above-mentioned raw material components, this invention can also prepare different cosmetic formulations, such as essence water, essence liquid, facial mask, essence lotion, essence cream, etc. In addition, based on the above-mentioned basic cosmetic categories, further derivative cosmetic categories can be obtained, which obviously all fall within the protection scope of this application.

[0017] The beneficial technical effects of the present application are:

[0018] 1. In vitro repair keratinization envelope test proves that one or two of the hyaluronic acid lysine copper chelate, the Dendrobium stem extract and the Glycyrrhiza inflata extract of the present application have synergistic effects, which can promote the expression of filaggrin (FLG), involucrin (IVL), loricrin (LOR), keratin (KRT1) and keratinocyte transglutaminase (TGM1) proteins, so as to achieve the effect of repairing keratinization envelope.

[0019] 2. The present application also unexpectedly finds that the composition also has the effect of promoting the formation of liquid crystal structure of the cream product.

[0020] 3. The present application proves by human efficacy experiment test that the skin care composition containing the keratinization envelope repair effect has good repair effect (after using the cosmetic containing 5.0% of the keratinization envelope repair composition for 4 weeks, the red area analysis a* value is significantly reduced, and the skin trans-epidermal water loss TEWL is significantly reduced). The preparation method of the cosmetic of the present application is simple and easy to operate, the raw materials are easy to obtain, and is suitable for mass production, so it has good practical application value. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 : Morphology diagram of Example 6 and Comparative Example 5 under polarizing microscope, wherein the left diagram is the liquid crystal structure diagram of Example 6, and the right diagram is the liquid crystal structure diagram of Comparative Example 5;

[0022] Figure 2 : Facial red area change diagram of No. 10 subject of Example 6 before and after using the sample (D0, D14 and D28);

[0023] Figure 3 : Facial red area change diagram of No. 26 subject of Example 6 before and after using the sample (D0, D14 and D28). DETAILED DESCRIPTION

[0024] The present application will be further described by the following examples, but the present application is not limited in the scope of the examples. Based on the examples in the present application, any change of the present application without creative labor is attributed to the protection scope of the present application. Meanwhile, in the examples of the present application, if no special description, all the preparation raw materials are the commercially available products well known by the skilled in the art.

[0025] Examples 1-5:

[0026] The skin care composition with keratinization envelope repairing effect of the present application is composed of hyaluronic acid lysine copper chelate, dendrobium stem extract and / or licorice extract, and the ratio of examples 1-5 and comparative examples 1-4 is shown in the following table 1.

[0027] Preparation method: mix hyaluronic acid lysine copper chelate, dendrobium stem extract and / or licorice extract (according to the ingredients and amount in table 1) together, and dissolve in purified water.

[0028] Table 1: ingredient list of examples 1-5 and comparative examples 1-4 (mass ratio)

[0029]

[0030] The hyaluronic acid lysine copper chelate used in the examples of the present application is obtained by example 1 of CN202410478415.3. Specifically, after mixing and uniformly dissolving sodium hyaluronate and lysine copper hydrochloride chelate, catalyst EDCI and NHS are added, and after incubation and reaction, an appropriate amount of ethanol is added for precipitation and purification, filtered, dehydrated with anhydrous ethanol, and then dried under reduced pressure to obtain blue granular powder, sample 1-6.

[0031] The preparation method of the dendrobium stem extract is as follows: fresh dendrobium stem is placed in a flash extractor, distilled water (solid-liquid ratio 1:20) is added, and extraction is carried out at 25℃, with 3 times of flash extraction, 90s / time, the extract is collected and concentrated to 1 / 5 of the original liquid volume in a flash concentrator, and then concentrated under reduced pressure using a rotary evaporator at 0.08MPa, 50-60℃, the concentrate is dissolved with an equal amount of butanediol, filtered with a 0.8μm filter to remove insoluble substances, and the obtained solution is the dendrobium stem extract. 7g of dendrobium stem extract is obtained from 1g of fresh dendrobium stem.

[0032] The preparation method of the dendrobium stem extract is as follows: fresh dendrobium stem is placed in a flash extractor, distilled water (solid-liquid ratio 1:20) is added, and extraction is carried out at 25℃, with 3 times of flash extraction, 90s / time, the extract is collected and concentrated to 1 / 5 of the original liquid volume in a flash concentrator, and then concentrated under reduced pressure using a rotary evaporator at 0.08MPa, 50-60℃, the concentrate is dissolved with an equal amount of butanediol, filtered with a 0.8μm filter to remove insoluble substances, and the obtained solution is the dendrobium stem extract. 7g of dendrobium stem extract is obtained from 1g of fresh dendrobium stem.

[0033] Example 6: serum

[0034] The above-mentioned skin care composition with keratinization envelope repairing effect is used to prepare serum products containing the keratinization envelope repairing composition, and the formula is shown in table 2.

[0035] Table 2: Formula of Essence Cream Products

[0036]

[0037] Preparation method of essence cream:

[0038] S1: Aqueous phase preparation: Add the raw materials from phase A separately, heat to 85℃ and stir to dissolve;

[0039] S2: Oil phase preparation: Add the raw materials from phase B separately, heat to 80℃ and stir to melt;

[0040] S3: Combine phase A and phase B, turn on homogenizer (3000 rpm), homogenize for 12 minutes, and start cooling;

[0041] S4: When the temperature drops to 45℃, add phase C and phase D components in sequence, stir to dissolve and disperse evenly, and the essence cream product is obtained.

[0042] The preparation method of Comparative Example 5 is the same as that of Example 6, except that Comparative Example 5 does not contain a skin care composition for repairing keratinized capsules, while the amount of keratinized capsule repair composition used in Example 6 is made up by adding water.

[0043] Example 1: In vitro expression of proteins related to the promotion of keratinization capsule formation, including FLG, LOR, IVL, KRT1, and TGM1.

[0044] use 3D skin models were used for experiments. According to the test protocol in Table 3, the models were transferred to 6-well plates, with three replicates per group. For groups BC and NC, 0.9 mL of EpiGrowth medium (Boxi Biotechnology) was added to each well, while for group PC, 0.9 mL of medium containing WY14643 (Sigma) was added to each well. The test group numbers were labeled on the 6-well plates. The working solution for the sample group was added to the model surface. UVB irradiation (irradiation dose of 600 mJ / cm²) was then applied to the groups requiring UVB irradiation according to the test protocol (Table 3). 2 The irradiation time was approximately 25 minutes. After irradiation, the model was placed in a CO2 incubator (37℃, 5% CO2) for 24 hours. After incubation, the surface of the model was cleaned with sterile PBS to remove any remaining test material, and residual liquid inside and outside the model was wiped away with sterile cotton swabs. The model used for testing was removed by circumferential cutting and fixed with 4% paraformaldehyde for 24 hours. Immunofluorescence or immunohistochemical detection of proteins related to keratinization capsule formation (FLG, LOR, IVL, KRT1, TGM1) was performed, and images were taken and analyzed under a microscope.

[0045] GraphPad Prism was used for plotting, and the results were expressed as Mean ± SD. The t-test statistical analysis was used for comparison between groups. The statistical analysis was two-tailed. It was considered to have significant difference when 0.01 < P < 0.05, and it was considered to have extremely significant difference when P < 0.01.

[0046] Table 3: Test scheme

[0047]

[0048] The dilution of the sample group was diluted with water.

[0049] Table 4: FLG content detection result summary table

[0050] Sample Name Mean SD P-value Upregulation rate (vs NC) BC 1.00 0.11 / / NC 0.35 0.06 0.001## / PC 0.71 0.08 0.003** 102.86％ Example 1 0.55 0.05 0.005** 57.14％ Example 2 0.78 0.03 0.000** 122.86％ Example 3 0.74 0.06 0.001** 111.43％ Example 4 0.61 0.09 0.005** 74.29％ Example 5 0.51 0.04 0.009** 45.71％ Comparative Example 1 0.48 0.01 0.005** 37.14％ Comparative Example 2 0.42 0.03 0.066 20.00％ Comparative Example 3 0.31 0.09 0.521 / Comparative Example 4 0.44 0.01 0.022* 25.71％

[0051] (Note: ## indicates p < 0.01 compared with the blank control; ** indicates p < 0.01 compared with the negative control; * indicates 0.01 < p < 0.05 compared with the negative control.)

[0052] As can be seen from Table 4, Examples 1-5, Comparative Example 1, Comparative Example 2, Comparative Example 4 have the function of promoting the expression of FLG protein, and single Glycyrrhiza inflata extract does not promote the expression of FLG protein. Example 2 has a synergistic effect after mixing hyaluronic acid lysine copper chelate, Dendrobium stem extract and Glycyrrhiza inflata extract, and the effect of promoting the expression of FLG protein is significantly higher than that of Examples 4, 5, Comparative Examples 1-4, indicating that the three have a significant synergistic effect in promoting the expression of FLG protein.

[0053] Table 5: LOR content detection result summary table

[0054]

[0055]

[0056] (Note: ## indicates p < 0.01 compared with the blank control; ** indicates p < 0.01 compared with the negative control; * indicates 0.01 < p < 0.05 compared with the negative control.)

[0057] As can be seen from Table 5, except for Comparative Example 3, the rest of the groups have a significant function of promoting the expression of LOR protein, and the effect of Example 2 on promoting the expression of LOR protein is significantly higher than that of Examples 4, 5, Comparative Examples 1-4 after mixing hyaluronic acid lysine copper chelate, Dendrobium stem extract and Glycyrrhiza inflata extract, indicating that the three have a significant synergistic effect in promoting the expression of LOR protein.

[0058] Table 6: IVL content detection result summary table

[0059] Sample Name Mean SD P-value Upregulation rate (vs NC) BC 1.00 0.11 / / NC 0.40 0.07 0.001## / PC 1.51 0.12 0.000** 277.50％ Example 1 0.69 0.09 0.008** 72.50％ Example 2 0.94 0.08 0.001** 135.00％ Example 3 0.84 0.05 0.000** 110.00％ Example 4 0.70 0.08 0.005** 75.00％ Example 5 0.61 0.04 0.004** 52.50％ Comparative Example 1 0.60 0.06 0.011* 50.00％ Comparative Example 2 0.49 0.01 0.044* 22.50％ Comparative Example 3 0.37 0.08 0.611 / Comparative Example 4 0.52 0.02 0.018* 30.00％

[0060] (Note: ## indicates p < 0.01 compared to the blank control; ** indicates p < 0.01 compared to the negative control; * indicates p < 0.01 compared to the negative control.) <p<0.05。)

[0061] As shown in Table 6, Comparative Example 3 did not promote IVL protein expression, indicating that the single extract of *Glycyrrhiza inflata* does not have the effect of promoting IVL protein expression. In Example 2, the synergistic effect of the mixture of hyaluronic acid-lysine copper chelate, *Dendrobium officinale* stem extract, and *Glycyrrhiza inflata* extract in promoting IVL protein expression was significantly higher than that in Examples 4, 5, Comparative Example 1, Comparative Example 2, and Comparative Example 4, indicating that the three have a significant synergistic promoting effect in promoting IVL protein expression.

[0062] Table 7: Summary of KRT1 Content Detection Results

[0063] Sample Name Mean SD P-value Upregulation rate (vs NC) BC 1.00 0.06 / / NC 0.52 0.03 0.000## / PC 1.03 0.10 0.001** 98.08％ Example 1 0.83 0.07 0.002** 59.62％ Example 2 1.10 0.05 0.000** 111.54％ Example 3 1.04 0.09 0.001** 100.00％ Example 4 0.65 0.06 0.025* 25.00％ Example 5 0.69 0.04 0.004** 32.69％ Comparative Example 1 0.42 0.06 0.061 / Comparative Example 2 0.65 0.04 0.012* 25.00％ Comparative Example 3 0.68 0.08 0.032* 30.77％ Comparative Example 4 0.81 0.05 0.001** 55.77％

[0064] (Note: ## indicates p < 0.01 compared to the blank control; ** indicates p < 0.01 compared to the negative control; * indicates p < 0.01 compared to the negative control.) <p<0.05。)

[0065] As shown in Table 7, Comparative Example 1 did not promote KRT1 protein expression, indicating that the single hyaluronic acid-lysine-copper chelate did not have the effect of promoting KRT1 protein expression. Example 2, which combined the hyaluronic acid-lysine-copper chelate, Dendrobium officinale stem extract, and Glycyrrhiza uralensis extract, showed a significantly higher synergistic effect in promoting KRT1 protein expression than Examples 4-5 and Comparative Examples 2-4, indicating that the three components have a significant synergistic promoting effect in promoting KRT1 protein expression.

[0066] Table 8: Summary of TGM1 Content Detection Results

[0067]

[0068]

[0069] (Note: ## indicates p < 0.01 compared to the blank control; ** indicates p < 0.01 compared to the negative control; * indicates p < 0.01 compared to the negative control.) <p<0.05。)

[0070] As can be seen from Table 8, Examples 1-5 and Comparative Examples 1-4 all have the function of promoting TGM1 protein expression. Meanwhile, Example 2, which combines hyaluronic acid lysine copper chelate, Dendrobium officinale stem extract and Glycyrrhiza uralensis extract, has a significantly higher synergistic effect on promoting KRT1 protein expression than Examples 4-5 and Comparative Examples 1-4. This indicates that the three have a significant synergistic effect on promoting KRT1 protein expression.

[0071] Experiment 2: Observation Experiment of Liquid Crystal Structure

[0072] Microscopic morphology images of the prepared samples from Example 6 and Comparative Example 5 were taken under a polarizing microscope. (See attached image for details.) Figure 1 It can be seen that the liquid crystal structure in Example 6 is significantly more abundant than that in Comparative Example 5. This also indicates that the composition with keratinization coating repair can promote the formation of liquid crystal structures in the system.

[0073] Test Example 3: Test Results of the Repair Effect of Cosmetics Containing the Skin Care Composition of the Present Invention on the Human Body

[0074] Example 6 was tested for its anti-aging efficacy on human skin. A total of 34 participants were tested, including 5 healthy Chinese men and 29 women aged 18 to 44 years, who met the criteria for voluntary inclusion and exclusion.

[0075] Directions for use: After cleansing and applying toner in the morning and evening, apply an appropriate amount of cream evenly to the face and gently massage until absorbed. Use twice daily, once in the morning and once in the evening, for 28 consecutive days.

[0076] Testing time: before using the sample (D0), 14 days after using the sample (D14), and 28 days after using the sample (D28).

[0077] Test methods and test parameters:

[0078] 1. Facial Imaging Test (VISIA-CR)

[0079] The VISIA-CR facial image analyzer (Canfield, USA) provides safe and stable standard lighting conditions. All camera settings and imaging parameters are software-controlled to ensure image stability and standardization. The instrument features multiple light source modes: standard light, blue light, orange light, cross-polarized light, and parallel-polarized light, enabling multi-angle imaging of facial skin with image quality up to 21 megapixels. It also performs image analysis on red zone images to obtain a quantitative indicator, the red zone analysis a* value, used to assess the improvement in facial skin redness and sensitivity.

[0080] 2. Skin moisture content test (Corneometer)

[0081] The Corneometer (CM 825, Courage and Khazaka, Germany) is a skin moisture meter that tests skin moisture content based on the principle of capacitance. It is used to detect the relative water content of the skin surface; the higher the value, the higher the water content.

[0082] 3. Transdermal Water Loss (TEWL) Test (Aqua Flux)

[0083] This test was used to detect transepidermal water loss (TWEL) values ​​in the skin and assess skin barrier function. A lower TWEL value indicates better water retention. Test results are shown in Tables 9-11.

[0084] Table 9: Descriptive statistics of a* values ​​in red zone analysis (n=34)

[0085]

[0086] Statistical analysis results: "-": no statistically significant difference (P≥0.05); "*": statistically significant difference (0.01≤P<0.05); "**": statistically significant difference (0.001≤P<0.01); "***": statistically significant difference (P<0.001).

[0087] Table 10: Descriptive statistics of skin moisture content (n=34)

[0088]

[0089] Statistical analysis results: "-": no statistically significant difference (P≥0.05); "*": statistically significant difference (0.01≤P<0.05); "**": statistically significant difference (0.001≤P<0.01); "***": statistically significant difference (P<0.001).

[0090] Table 11 Descriptive statistics of transcutaneous water loss (TEWL) values ​​(n=34)

[0091]

[0092] Statistical analysis results: "-": no statistically significant difference (P≥0.05); "*": statistically significant difference (0.01≤P<0.05); "**": statistically significant difference (0.001≤P<0.01); "***": statistically significant difference (P<0.001).

[0093] As shown in Tables 9-11, during the 4-week testing period, Example 6 has a significant moisturizing effect that increases skin moisture content and a repairing and redness-reducing effect that lowers TEWL value and redness a*. Therefore, the essence cream containing the skin care composition with keratinization capsule repair effect has a good repairing effect.

[0094] The facial redness changes of subjects No. 10 and No. 26 in Example 6 before and after sample use (D0, D14, and D28) are shown in the following figures. Figures 2-3 As shown in the figure, the essence cream containing skincare composition with keratin capsule repair function has significant repair and redness reduction effects.

[0095] It should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the given examples, those skilled in the art can modify or make equivalent substitutions to the technical solutions of the present invention as needed, without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A skincare composition with keratinized capsule repair function, characterized in that, The product contains the following components in parts by weight: 0.05-0.5 parts of hyaluronic acid-lysine-copper chelate, 1.0-10.0 parts of Dendrobium officinale stem extract, and 1.0-10.0 parts of Glycyrrhiza uralensis extract; The preparation method of the extract of Glycyrrhiza inflata is as follows: pulverize dried Glycyrrhiza inflata, add 60%-80% ethanol solution, heat and reflux at 70-80℃ for 2-4 times, and combine the filtrates; concentrate the filtrate under reduced pressure, volatilize until there is no obvious ethanol odor, add 1.5-2.0 times the mass of the concentrate of butanediol, stir evenly, filter, and obtain the extract of Glycyrrhiza inflata, wherein the concentration, based on the crude drug, is 0.25-0.40 g / mL; The Dendrobium officinale stem extract is prepared by: placing fresh Dendrobium officinale stems in a flash extractor, adding distilled water, and extracting at room temperature; combining the extracts, concentrating, adding 1-2 times the mass of butanediol to the concentrate, stirring evenly, filtering, and removing insoluble substances. The resulting solution is the Dendrobium officinale stem extract. 6-10g of Dendrobium officinale stem extract is obtained from every 1g of fresh Dendrobium officinale stems.

2. The skincare composition as described in claim 1, characterized in that, Hyaluronic acid-lysine copper chelate, Dendrobium officinale stem extract and Glycyrrhiza uralensis extract were mixed together and dissolved in purified water to make up to 100 parts.

3. The use of the skincare composition according to claim 1 in the preparation of cosmetics with skincare effects of repairing keratinized capsules.

4. The application as described in claim 3, characterized in that, By promoting the expression of proteins related to keratin capsule formation, such as filaggrin, inner lining protein, clasping protein, and keratin, it effectively repairs the keratin capsule and the skin barrier.

5. A cosmetic product with skin-care effects of repairing keratinized capsules, characterized in that, The cosmetic contains 0.5% to 30% of the skin care composition according to claim 2.

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