A pesticide composition containing fenpyrazone, a pesticide preparation and its application in weed control

Abstract

The present invention belongs to the technical field of pesticide preparation, specifically relating to a pesticide composition containing fenpyrazone, a pesticide formulation, and its use in weed control. The pesticide composition, prepared by combining fenpyrazone with a herbicide, including an HPPD inhibitor, exhibits excellent weed control efficacy, significantly exceeding the theoretical sum of the effects of a single agent. While the dosage of the active ingredient is reduced, the herbicidal activity is enhanced, demonstrating a synergistic effect. Furthermore, compared to single-agent herbicides, the composition of the present invention utilizes a lower dosage of the active ingredient, avoiding residual toxicity to rice, surrounding crops, and subsequent crops, while also reducing costs.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticide preparation, and in particular relates to a pesticide composition containing fenpyrazone, a pesticide preparation and application thereof in weed control. Background Art

[0002] Currently, chemical control is the most convenient and effective method for controlling weeds in rice fields. The most serious weeds in rice fields are grass weeds, represented by Echinochloa crusgalli, Leptochloa chinensis, Digitaria sanguinalis, weedy rice, and spurious rice. These weeds are difficult to control due to their biological and ecological characteristics, seedling morphology, and other characteristics that closely resemble those of rice. Echinochloa crusgalli is the most common noxious weed in major rice-producing areas, with Leptochloa crusgalli and weedy rice ranking second and third, respectively. Spurious rice and Digitaria sanguinalis have also become new dominant weeds in rice fields, with the potential to evolve into noxious weeds. Therefore, resolving the challenge of controlling grass weeds in rice fields resolves the primary contradiction in weed control.

[0003] 4-Hydroxyphenyl-pyruvate dioxygenase (HPPD) is an important target and a popular choice for herbicide development. The main commercialized varieties include sulcotrione, mesotrione, benzobicyclon, tripyrasulfone, mesotrione, isoxaflutole, topramezone, flusulfinam, tembotrione, bipyrazone, cypyrafluone, fenpyrazone, tefuryltrione, isoxaflutole and bicyclopyrone. HPPD inhibitor herbicides are mainly used for crops such as corn, rice, and sugarcane. The main characteristics of HPPD herbicides are low resistance risk, high activity, broad weed control spectrum, and diverse chemical structures.

[0004] Benpyrazone is the first benzoate pyrazolone herbicide. When used in cornfields, it effectively controls or suppresses the following weeds: crabgrass, barnyardgrass, goosegrass, foxtail grass, wild millet, quinoa, polyanthus, velvetleaf, amaranthus retroflexus, ragweed, datura, cyperus rotundus, purslane, cocklebur, nightshade, and red sedge. However, it is less effective against sedge weeds. While benpyrazone can effectively control a variety of rice field weeds, including crabgrass and barnyardgrass, when used in cornfields, its safety profile in rice is poor, and its weed control effectiveness is also less than ideal. Summary of the Invention

[0005] The present invention aims to provide a pesticide composition containing fenpyrazone, a pesticide formulation, and their use in weed control. The pesticide composition and the pesticide formulation containing the pesticide composition can effectively control weeds in rice fields, can reduce weed resistance on the basis of synergistic enhancement, have a positive effect on reducing the dosage of the drug, and are safer for rice.

[0006] The invention provides a pesticide composition containing benzathine, comprising a first component and a second component, wherein the first component is benzathine, and the second component comprises triazosulfuron, quinpyraclostrobin, pyrazosulfuron-methyl or dithiopyr; and the mass ratio of the first component to the second component is 1:0.5-100.

[0007] The present invention also provides the use of the pesticide composition described in the above technical solution in pesticides.

[0008] The present invention also provides a pesticide preparation containing fenpyrazone, comprising auxiliary materials and an effective dose of the pesticide composition described in the above technical solution.

[0009] Preferably, the mass of the pesticide composition is 8%-72% of the total mass of the pesticide formulation.

[0010] Preferably, the mass of the pesticide composition is 12%-54% of the total mass of the pesticide formulation.

[0011] Preferably, the pesticide formulation includes a wettable powder, emulsifiable concentrate, suspension concentrate or aqueous emulsion.

[0012] The present invention also provides the use of the pesticide composition described in the above technical solution or the pesticide preparation described in the above technical solution in controlling weeds.

[0013] Preferably, the weeds include weeds in rice fields; and the types of weeds include grass weeds.

[0014] Preferably, the grass weeds include one or more of Echinochloa spp., Leptochloa chinensis, Digitaria sanguinalis, weedy rice and false rice.

[0015] The present invention provides a method for controlling weeds in rice fields, comprising: spraying the pesticide formulation described in the above technical solution on the stems and leaves of rice after the rice reaches the 4-leaf stage;

[0016] Based on the dosage of the pesticide composition in the pesticide preparation, the dosage of the pesticide preparation is 61.5-672 g a.i. / hm 2 . .

[0017] Beneficial effects:

[0018] The present invention provides a pesticide composition containing fenpyrazone, comprising a first component and a second component, wherein the first component is fenpyrazone, and the second component comprises triazosulfuron, pyraclostrobin, pyrazosulfuron-methyl or dithiopyrazone; the mass ratio of the first component to the second component is 1:0.5-100. The pesticide composition prepared by compounding fenpyrazone with herbicides including HPPD inhibitor herbicides has excellent weed control effect and is significantly better than the sum of the theoretical effects of a single agent. When the amount of active ingredient is reduced, the herbicidal activity is improved, and there is a synergistic effect. In addition, compared with a single-agent herbicide, the pesticide composition of the present invention uses a lower amount of active ingredient, thereby avoiding residual toxicity to rice, surrounding crops and subsequent crops, while reducing costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments are briefly introduced below.

[0020] Figure 1 The synergistic effect of the combination of fenpyraclostrobin and triazone in controlling Echinochloa weeds in Example 1;

[0021] Figure 2 The synergistic effect of the combination of fenpyraclostrobin and triazone in controlling Leptochloa chinensis in Example 1;

[0022] Figure 3 The synergistic effect of the combination of fenpyraclostrobin and triazone in controlling crabgrass in Example 1 is shown;

[0023] Figure 4 The synergistic effect of the combination of fenpyraclostrobin and quinclorac-butyl in controlling Echinochloa weeds in Example 2;

[0024] Figure 5 The synergistic effect of the combination of fenpyraclostrobin and quinclorac-butyl in controlling Leptochloa chinensis in Example 2;

[0025] Figure 6 The synergistic effect of the combination of fenpyraclostrobin and quinpyraclostrobin in controlling crabgrass in Example 2;

[0026] Figure 7 The synergistic effect of the combination of benzathine and pyrazosulfuron-methyl in controlling Echinochloa weeds in Example 3;

[0027] Figure 8 The synergistic effect of the combination of benzathine and pyrazosulfuron-methyl in controlling Leptochloa chinensis in Example 3;

[0028] Figure 9 The synergistic effect of the combination of benzathine and pyrazosulfuron-methyl in controlling crabgrass in Example 3;

[0029] Figure 10The synergistic effect of the combination of fenpyraclostrobin and dithiopyr in controlling Echinochloa weeds in Example 4;

[0030] Figure 11 The synergistic effect of the combination of fenpyraclostrobin and dithiopyr in controlling Leptochloa chinensis in Example 4;

[0031] Figure 12 This is the synergistic effect of the combination of fenpyraclostrobin and dithiopyr in controlling crabgrass in Example 4. DETAILED DESCRIPTION

[0032] The invention provides a pesticide composition containing benzathine, comprising a first component and a second component, wherein the first component is benzathine, and the second component comprises triazosulfuron, quinpyraclostrobin, pyrazosulfuron-methyl or dithiopyr; and the mass ratio of the first component to the second component is 1:0.5-100.

[0033] The mass ratio of the first component to the second component of the present invention is preferably any value in the range of 1:0.5-100, specifically preferably 1:10-80, further preferably 1:20-60, more preferably 1:25-40, and most preferably 1:30-36.

[0034] The present invention also provides the use of the pesticide composition described in the above technical solution in pesticides.

[0035] Specifically, the present invention also provides a pesticide formulation containing fenpyrazone, comprising excipients and an effective dose of the pesticide composition described in the above technical solution. The mass of the pesticide composition of the present invention preferably accounts for 8%-72% of the total mass of the pesticide formulation, more preferably 12%-54%, and even more preferably 30%-42%. The pesticide formulation of the present invention preferably comprises a wettable powder, emulsifiable concentrate, suspension concentrate, or emulsion in water; the suspension concentrate preferably comprises a microcapsule suspension concentrate. The present invention does not specifically limit the type, dosage, or source of the excipients in the pesticide formulation; they can be conventionally selected based on the pesticide formulation being prepared. For example, in one embodiment of the present invention, a 30.5% benzathine-triazole emulsifiable concentrate contains the following excipients by weight: 4% sodium lauryl sulfate, 3% alkylphenol polyoxyethylene ether, and 62.5% solvent oil. In another embodiment of the present invention, a 41% benzathine-triazole suspension concentrate contains the following excipients by weight: 4% polyoxyethylene sorbitan fatty acid ketone, 3% alkylphenol formaldehyde resin polyoxyethylene ether, 1% ethylene glycol, and 51% water. In another embodiment of the present invention, a 37% benzathine-triazole pyrazosulfuron aqueous emulsion contains the following excipients by weight: 4% xylene, 3% calcium dodecylbenzenesulfonate, 4% sodium dioctyl sulfosuccinate, 2% glycerol, and 50% water. In another embodiment of the present invention, a 42% fenpyraclostrobin-dithiopyracil suspension contains the following excipients in percentage by weight: 5% polyoxyethylene sorbitan fatty acid ketone, 4% alkylphenol formaldehyde resin polyoxyethylene ether, 1% ethylene glycol and 48% water.

[0036] The present invention also provides use of the pesticide composition or pesticide formulation described in the above technical solution for controlling weeds. The weeds described in the present invention are preferably weeds in rice fields, and the weeds include grass weeds, more preferably one or more of Echinochloa genus, Leptochloa chinensis, Digitaria sanguinalis, weedy rice, and false rice. The rice fields described in the present invention are preferably japonica rice fields.

[0037] The present invention also provides a method for controlling weeds in rice fields, comprising: spraying the pesticide formulation described in the above technical solution on the stems and leaves of rice after the rice reaches the 4-leaf stage;

[0038] Based on the dosage of the pesticide composition in the pesticide preparation, the dosage of the pesticide preparation is 61.5-672 g a.i. / hm 2 The pesticide preparation of the present invention preferably includes but is not limited to 30.5% benzathone·tripyram sulfone emulsifiable concentrate, 41% benzathone·pyraclostrobin suspension concentrate, 37% benzathone·pyraclostrobin aqueous emulsion or 42% benzathone·dithiopyrazone suspension concentrate. When the pesticide preparation is 30.5% benzathone·tripyram sulfone emulsifiable concentrate, the dosage is preferably 300-500 mL / hm2 Calculated on the basis of active ingredients, the dosage is preferably 91.5-152.5 g ai / hm 2 , more preferably 91.5-122g ai / hm 2 When the pesticide preparation is 41% benzathine-pyraclostrobin suspension, the dosage is preferably 150-250 mL / hm 2 Calculated on the basis of active ingredients, the dosage is preferably 61.5-102.5 g ai / hm 2 , more preferably 61.5-82 g a.i. / hm 2 When the pesticide formulation is 37% benzathine·pyrazosulfuron-ethyl emulsion in water, the dosage is preferably 300-500mL / hm 2 Calculated on the basis of active ingredients, the dosage is preferably 111-185g ai / hm 2 , more preferably 111-148g ai / hm 2 When the pesticide formulation is 42% benzathine·dithiopyra suspension, the dosage is preferably 600-1000mL / hm 2 Calculated on the basis of active ingredients, the dosage is preferably 252-420 g ai / hm 2 , more preferably 252-336g ai / hm 2 The rice of the present invention is preferably japonica rice to ensure the safety of the rice. The pesticide formulation is preferably applied after the rice reaches the 4-leaf stage to further ensure the safety of the rice.

[0039] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below with reference to the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0040] Example 1

[0041] Experiment on the synergistic effect of different combinations of fenpyrasulfone and triazone and its control effect on grass weeds

[0042] 1. Test conditions

[0043] 1.1 Test targets

[0044] In the embodiment, the most representative and difficult to control weeds in rice fields, Echinochloa crus-galli, Leptochloa chinensis, and the new dominant weed, Digitaria sanguinalis, are used as examples to illustrate in detail. The seeds of Echinochloa crus-galli, Leptochloa crus-galli, and Digitaria sanguinalis used were all collected from rice fields.

[0045] Use the potting method. Use 140×140mm plastic pots placed on trays filled with air-dried, sieved topsoil collected from the field. Add water directly to the top of the pots to maintain saturated soil moisture initially. Select seeds of Echinochloa, Leptochloa chinensis, and Digitaria with plump, uniform seeds. Germinate in a 30°C incubator (dark). Place newly white weed seeds evenly on the soil surface and cover with 0.5cm of soil. Keep the soil moist after sowing and cultivate in a controlled sunlight greenhouse.

[0046] 1.2 Culture conditions

[0047] The growth was carried out in a controlled sunlight greenhouse with a night temperature of 15-20°C, a day temperature of 25-30°C, natural light, and a relative humidity of 60%-75%. The soil type was loam with an organic matter content of 1.8% and a pH of 7.1.

[0048] 1.3 Instruments and Equipment

[0049] ASS-4 automatic quantitative spray system, GA110 1 / 10,000 electronic balance;

[0050] ZDR2000 intelligent data logger, MLR-352H plant incubator.

[0051] 2. Experimental Design

[0052] 2.1 Test Agents

[0053] The 98% mass concentration of fenpyrasulfone technical drug and the 95% mass concentration of triazosulfone technical drug are both prepared in acetone and diluted with a 0.1% Tween 80 aqueous solution. Dilute immediately before use.

[0054] 2.2 Experimental treatment

[0055] 2.2.1 Dose setting

[0056] Based on the respective activities of the two agents and the characteristics of laboratory testing, different mixed combinations and single-dose technical agents were set up based on the initial laboratory test. The highest formulation of fenpyroxene was 14.4g ai / hm 2 , the dilution was performed in a multiple of 2, and the final dose settings were 14.4, 7.20, 3.60, 1.80 and 0.90 g ai / hm 2 At the same time, water without drug and containing the same emulsifier was used as blank control (0 g ai / hm 2 ); the highest formulation of triazosulfuron was 1440g ai / hm 2 , the dilution was performed in a multiple of 4, and the final dose settings were 1440, 360, 90, 22.5 and 5.62 g ai / hm 2At the same time, water without the drug and containing the same emulsifier was used as a blank control. Six doses of the two drugs were combined in pairs, for a total of 36 combinations, and then the indoor bioassay was carried out.

[0057] 2.2.2 Experimental repetition

[0058] Each treatment involved 3 pots, 10 weed seeds were sown in each pot, and there were 30 weed plants in each treatment. The experiment was repeated twice.

[0059] 3. Handling Methods

[0060] 3.1 Processing time and frequency

[0061] The drug was applied once in the experiment. When the weeds grew to the 4-leaf stage, the weeds were thinned out to keep 8 weeds in each pot, and 24 weeds were retained for each treatment.

[0062] 3.2 Instruments and medication methods

[0063] Weeds were evenly distributed on a platform and sprayed using an ASS-4 automatic quantitative spray system equipped with a TeeJet XR8002VS fan nozzle at a spray rate of 450 L / ha and a spray pressure of 0.275 MPa. After 30 seconds of spraying, the spray tower door was opened and the nutrient pots were removed. The air valve was then opened and 100 mL of clean water was sprayed to clean the spray pipe.

[0064] 4. Test methods

[0065] The test was carried out in accordance with the Guidelines for Indoor Bioassay Tests of Pesticides - NY / T1155.9-2008 and NY / T1155.7-2006.

[0066] 5. Data investigation and statistical analysis

[0067] 5.1 Survey Methods

[0068] Because the experimental concentration gradients were inconsistent, weeds at low concentrations were not completely killed. Therefore, the absolute number survey method was used to investigate the fresh weight of weeds. Surviving weed seedlings were cut along the soil surface with scissors and the fresh weight of the weeds was weighed using an analytical balance.

[0069] 5.2 Survey duration and frequency

[0070] The survey was conducted 21 days after treatment, for a total of 1 survey.

[0071] 5.3 Data Statistical Analysis

[0072] The logarithm of the dosage and the probability of the control effect were analyzed by DPS statistical software, and the herbicide dosage that inhibited the growth of weeds by 90% (GR 90 The isobologram method was used to determine whether the mixture had a synergistic effect. There was no significant difference between the two replicates.

[0073] The results of the indoor tests are shown in Table 1-3. The results of the synergistic effect determination using the isoequivalent line method are shown in Table 1-3. Figure 1-3 shown

[0074] Table 1 Synergistic effect of the combination of fenpyraclostrobin and triazosulfuron on controlling Echinochloa weeds

[0075]

[0076]

[0077] From Table 1 and Figure 1 It can be seen that compared with the single doses of the two, the combination of fenpyrazone and triazosulfone has a significant synergistic effect on the fresh weight inhibition of Echinochloa spp. All points are located below the isobol line, indicating that each formulation ratio in Example 1 has a synergistic effect, and the maximum synergistic point is (2.94, 90). When the dosage of fenpyrazone and triazosulfone reaches 7.2+90g ai / hm 2 When the amount of the compound is less than 1%, the fresh weight control effect on Echinochloa weeds can reach 100%.

[0078] Table 2 Synergistic effect of combination of fenpyraclostrobin and triazone on controlling Leptochloa chinensis

[0079]

[0080] From Table 2 and Figure 2 It can be seen that compared with the single doses of the two, the combination of fenpyraclostrobin and triazosulfuron has a significant synergistic effect on the fresh weight inhibition of Leptochloa chinensis. All points are located below the isobol line, indicating that each formula ratio in the example has a synergistic effect, and the maximum synergistic point is (1.98, 1440). When the dosage of fenpyraclostrobin and triazosulfuron reaches 14.4+1440g ai / hm 2 When used, the fresh weight protection effect on Leptochloa chinensis can reach 100%.

[0081] Table 3 Synergistic effect of the combination of fenpyraclostrobin and triazosulfuron on controlling crabgrass

[0082]

[0083] From Table 3 and Figure 3 It can be seen that compared with the single doses of the two, the combination of fenpyrazone and triazosulfone has a significant synergistic effect on the fresh weight inhibition of crabgrass. All points are located below the isobol line, indicating that each formula ratio in the example has a synergistic effect, and the maximum synergistic point is (4.45, 1440). When the dosage of fenpyrazone and triazosulfone reaches 14.4+1440 g ai / hm 2 When the fresh weight of crabgrass is controlled, the control effect can reach 100%.

[0084] Taking into account the comprehensive control effect, reduction requirements and cost, the ratio of the two is set at 1:60.

[0085] Example 2

[0086] Experiment on the synergistic effect of different ratios of fenpyraclostrobin and pyraclostrobin and their control effect on grass weeds

[0087] 1 Test conditions

[0088] 1.1 Test targets

[0089] The test examples are detailed using Echinochloa crus-galli and Leptochloa chinensis, two of the most representative and difficult weeds to control in rice fields, as well as the newly dominant weed Digitaria sanguinalis. The seeds of the Echinochloa crus-galli, Leptochloa crus-galli, and Digitaria sanguinalis were all collected from rice fields.

[0090] Use the potting method. Use 140×140mm plastic pots placed on trays filled with air-dried, sieved topsoil collected from the field. Add water directly to the top of the pots to maintain saturated soil moisture initially. Select seeds of Echinochloa, Leptochloa chinensis, and Digitaria with plump, uniform seeds. Germinate in a 30°C incubator (dark). Place newly white weed seeds evenly on the soil surface and cover with 0.5cm of soil. Keep the soil moist after sowing and cultivate in a controlled sunlight greenhouse.

[0091] 1.2 Culture conditions

[0092] The growth was carried out in a controlled sunlight greenhouse with a night temperature of 15-20°C, a day temperature of 25-30°C, natural light, and a relative humidity of 60%-75%. The soil type was loam with an organic matter content of 1.8% and a pH of 7.1.

[0093] 1.3 Instruments and Equipment

[0094] ASS-4 automatic quantitative spray system, GA110 1 / 10,000 electronic balance;

[0095] ZDR2000 intelligent data logger, MLR-352H plant incubator.

[0096] 2. Experimental Design

[0097] 2.1 Test Agents

[0098] The 98% mass concentration of fenpyraclostrobin technical and the 100% mass concentration of quinpyraclostrobin standard are both prepared in acetone and diluted with a 0.1% Tween 80 aqueous solution. Dilute immediately before use.

[0099] 2.2 Experimental treatment

[0100] 2.2.1 Dose setting

[0101] Based on the respective activities of the two agents and their laboratory test characteristics, different mixed combinations and a single dose of the technical agent were set up based on the initial laboratory test. Indoor bioassays were conducted using water containing no agent and the same emulsifier as blank controls. The highest formulation of fenpyroxene was 14.4g ai / hm2. 2 , the dilution was performed in a multiple of 2, and the final dose settings were 14.4, 7.20, 3.60, 1.80 and 0.90 g ai / hm 2 At the same time, water without drug and containing the same emulsifier was used as blank control; the highest concentration of pyraclostrobin was 1600g ai / hm 2 , the dilution was performed in a multiple of 4, and the final dose was set as 1600, 400, 100, 25 and 6.25 g ai / hm 2 At the same time, water without the drug and containing the same emulsifier was used as a blank control. Six doses of the two drugs were combined in pairs, for a total of 36 combinations, and then the indoor bioassay was carried out.

[0102] 2.2.2 Experimental Replication

[0103] Each treatment involved 3 pots, 10 weed seeds were sown in each pot, and there were 30 weed plants in each treatment. The experiment was repeated twice.

[0104] 3. Handling Methods

[0105] 3.1 Processing time and frequency

[0106] The drug was applied once in the experiment. When the weeds grew to the 4-leaf stage, the weeds were thinned out to keep 8 weeds in each pot, and 24 weeds were retained for each treatment.

[0107] 3.2 Instruments and medication methods

[0108] Weeds were evenly distributed on a platform and sprayed using an ASS-4 automatic quantitative spray system equipped with a TeeJet XR8002VS fan nozzle at a spray rate of 450 L / ha and a spray pressure of 0.275 MPa. After 30 seconds of spraying, the spray tower door was opened and the nutrient pots were removed. The air valve was then opened and 100 mL of clean water was sprayed to clean the spray pipe.

[0109] 4. Test methods

[0110] The test was carried out in accordance with the Guidelines for Indoor Bioassay Tests of Pesticides - NY / T1155.9-2008 and NY / T1155.7-2006.

[0111] 5. Data investigation and statistical analysis

[0112] 5.1 Survey Methods

[0113] Because the experimental concentration gradients were inconsistent, weeds at low concentrations were not completely killed. Therefore, the absolute number survey method was used to investigate the fresh weight of weeds. Surviving weed seedlings were cut along the soil surface with scissors and the fresh weight of the weeds was weighed using an analytical balance.

[0114] 5.2 Survey duration and frequency

[0115] The survey was conducted 15 days after treatment, for a total of 1 survey.

[0116] 5.3 Data Statistical Analysis

[0117] The logarithm of the dosage and the probability of the control effect were analyzed by DPS statistical software, and the herbicide dosage that inhibited the growth of weeds by 90% (GR 90 The isobologram method was used to determine whether the mixture had a synergistic effect. There was no significant difference between the two replicates.

[0118] The results of the indoor tests are shown in Table 4-6 below. The results of the synergistic effect determination using the equivalent line method are as follows: Figure 4-6 As shown:

[0119] Table 4 Synergistic effect of combination of fenpyraclostrobin and pyraclostrobin in controlling Echinochloa weeds

[0120]

[0121]

[0122] From Table 4 and Figure 4 It can be seen that compared with the single doses of the two, the combination of benzathine and pyraclostrobin has a significant synergistic effect on the fresh weight inhibition of Echinochloa spp. All points are located below the isobol line, indicating that each formulation ratio in the example has a synergistic effect, and the maximum synergistic point is (2.31, 100). When the dosage of benzathine and pyraclostrobin reaches 14.40+25g ai / hm 2 When the amount of the compound is less than 1%, the fresh weight control effect on Echinochloa weeds can reach 100%.

[0123] Table 5 Synergistic effect of combination of fenpyraclostrobin and pyraclostrobin on controlling Leptochloa chinensis

[0124]

[0125] From Table 5 and Figure 5 It can be seen that compared with the single doses of the two, the combination of fenpyraclostrobin and pyraclostrobin has a significant synergistic effect on the fresh weight inhibition of Leptochloa chinensis. All points are located below the isobol line, indicating that each formula ratio in the example has a synergistic effect, and the maximum synergistic point is (4.30, 90). When the dosage of fenpyraclostrobin and pyraclostrobin reaches 14.4+100g ai / hm 2When used, the fresh weight protection effect on Leptochloa chinensis can reach 100%.

[0126] Table 6 Synergistic effect of combination of fenpyraclostrobin and pyraclostrobin in controlling crabgrass

[0127]

[0128]

[0129] From Table 6 and Figure 6 It can be seen that compared with the single doses of the two, the combination of fenpyraclostrobin and pyraclostrobin has a significant synergistic effect on the fresh weight inhibition of crabgrass. All points are located below the isobol line, indicating that each formula ratio in the example has a synergistic effect, and the maximum synergistic point is (7.2, 47.13). When the dosage of fenpyraclostrobin and pyraclostrobin reaches 7.2+400g ai / hm 2 When the fresh weight of crabgrass is controlled, the control effect can reach 100%.

[0130] Taking into account the comprehensive control effect, reduction requirements and cost, the ratio of the two is set at 1:40.

[0131] Test Example 3

[0132] Experiment on the synergistic effect of different ratios of benzathine and pyrazosulfuron-methyl and their control effects on grass weeds

[0133] 1 Test conditions

[0134] 1.1 Test targets

[0135] The test examples are detailed using Echinochloa crus-galli and Leptochloa chinensis, two of the most representative and difficult weeds to control in rice fields, as well as the newly dominant weed Digitaria sanguinalis. The seeds of the Echinochloa crus-galli, Leptochloa crus-galli, and Digitaria sanguinalis were all collected from rice fields.

[0136] Use the potting method. Use 140×140mm plastic pots placed on trays filled with air-dried, sieved topsoil collected from the field. Add water directly to the top of the pots to maintain saturated soil moisture initially. Select seeds of Echinochloa, Leptochloa chinensis, and Digitaria with plump, uniform seeds. Germinate in a 30°C incubator (dark). Place newly white weed seeds evenly on the soil surface and cover with 0.5cm of soil. Keep the soil moist after sowing and cultivate in a controlled sunlight greenhouse.

[0137] 1.2 Culture conditions

[0138] The growth was carried out in a controlled sunlight greenhouse with a night temperature of 15-20°C, a day temperature of 25-30°C, natural light, and a relative humidity of 60%-75%. The soil type was loam with an organic matter content of 1.8% and a pH of 7.1.

[0139] 1.3 Instruments and Equipment

[0140] ASS-4 automatic quantitative spray system, GA110 1 / 10,000 electronic balance;

[0141] ZDR2000 intelligent data logger, MLR-352H plant incubator.

[0142] 2. Experimental Design

[0143] 2.1 Test Agents

[0144] The mass concentration of triazosulfuron technical drug is 95%, and the mass concentration of pyrazosulfuron-methyl technical drug is 98%.

[0145] The solvents of both original drugs are acetone, which is diluted with a 0.1% Tween 80 aqueous solution and diluted immediately before use.

[0146] 2.2 Experimental treatment

[0147] 2.2.1 Dose setting

[0148] Based on the respective activities of the two agents and the characteristics of laboratory testing, different mixed combinations and single-dose technical agents were set up based on the initial laboratory test. The highest formulation of fenpyroxene was 14.4g ai / hm 2 , the dilution was performed in a multiple of 2, and the final dose settings were 14.4, 7.20, 3.60, 1.80 and 0.90 g ai / hm 2 , set up blank control at the same time; the highest preparation of pyrazosulfuron-methyl was 1440g ai / hm 2 , the dilution was performed in a multiple of 4, and the final dose settings were 1440, 360, 90, 22.5 and 5.62 g ai / hm 2 , and a blank control was set up at the same time. The six doses of the two drugs were combined in pairs, for a total of 36 combinations.

[0149] Indoor bioassays were carried out using water without the drug or with the same emulsifier as blank controls.

[0150] 2.2.2 Experimental repetition

[0151] Each treatment was replicated 4 times, with 3 pots per treatment and 10 weed seeds sown in each pot, for a total of 30 plants per treatment.

[0152] 3. Handling Methods

[0153] 3.1 Processing time and frequency

[0154] The drug was applied once in the experiment. When the weeds grew to the 4-leaf stage, the weeds were thinned out to keep 8 weeds in each pot, and 24 weeds were retained for each treatment.

[0155] 3.2 Instruments and medication methods

[0156] Weeds were evenly distributed on a platform and sprayed using an ASS-4 automatic quantitative spray system equipped with a TeeJet XR8002VS fan nozzle at a spray rate of 450 L / ha and a spray pressure of 0.275 MPa. After 30 seconds of spraying, the spray tower door was opened and the nutrient pots were removed. The air valve was then opened and 100 mL of clean water was sprayed to clean the spray pipe.

[0157] 4. Test methods

[0158] The test was carried out in accordance with the Guidelines for Indoor Bioassay Tests of Pesticides - NY / T1155.9-2008 and NY / T1155.7-2006.

[0159] 5. Data investigation and statistical analysis

[0160] 5.1 Survey Methods

[0161] Because the experimental concentration gradients were inconsistent, weeds at low concentrations were not completely killed. Therefore, the absolute number survey method was used to investigate the fresh weight of weeds. Surviving weed seedlings were cut along the soil surface with scissors and the fresh weight of the weeds was weighed using an analytical balance.

[0162] 5.2 Survey duration and frequency

[0163] The survey was conducted 15 days after treatment, for a total of 1 survey.

[0164] 5.3 Data Statistical Analysis

[0165] The logarithm of the dosage and the probability of the control effect were analyzed by DPS statistical software, and the herbicide dosage that inhibited the growth of weeds by 90% (GR 90 The isobologram method was used to determine whether the mixture had a synergistic effect. There was no significant difference between the two replicates.

[0166] The results of the indoor tests are shown in Tables 7-9. The results of the synergistic effect determination using the isoequivalent line method are shown in Tables 7-9. Figure 7-9 As shown:

[0167] Table 7 Synergistic effect of the combination of benzathine and pyrazosulfuron-methyl on controlling Echinochloa weeds

[0168]

[0169] From Table 7 and Figure 7It can be seen that compared with the single agent of the two, the combination of benzathine and pyrazosulfuron has a significant synergistic effect on the fresh weight inhibition of Echinochloa spp. All points are located below the isobol line, indicating that each formula ratio in the example has a synergistic effect, and the maximum synergistic point is (1.8, 148.33). When the dosage of benzathine and pyrazosulfuron reaches 14.4+90g ai / hm 2 When the amount of the compound is less than 1%, the fresh weight control effect on Echinochloa weeds can reach 100%.

[0170] Table 8 Synergistic effect of combination of fenpyraclostrobin and pyrazosulfuron-methyl on controlling Leptochloa chinensis

[0171]

[0172]

[0173] From Table 8 and Figure 8 It can be seen that compared with the single doses of the two, the combination of benzathine and pyrazosulfuron-methyl has a significant synergistic effect on the fresh weight inhibition of Leptochloa chinensis. All points are located below the isobol line, indicating that each formula ratio in the example has a synergistic effect, and the maximum synergistic point is (10.25, 360). When the dosage of benzathine and pyrazosulfuron-methyl reaches 14.4+1440g ai / hm 2 When used, the fresh weight protection effect on Leptochloa chinensis can reach 100%.

[0174] Table 9 Synergistic effect of combination of benzathine and pyrazosulfuron-methyl on controlling crabgrass

[0175]

[0176] From Table 9 and Figure 9 It can be seen that compared with the single doses of the two, the combination of benzathine and pyrazosulfuron-methyl has a significant synergistic effect on the fresh weight inhibition of crabgrass. All points are located below the isobol line, indicating that each formula ratio in the example has a synergistic effect, and the maximum synergistic point is (4.08, 90). When the dosage of benzathine and pyrazosulfuron reaches 14.4+90g ai / hm 2 When used, the fresh weight protection effect on Leptochloa chinensis can reach 100%.

[0177] Taking into account the comprehensive control effect, reduction requirements and cost, the ratio of the two is set at 1:36.

[0178] Test Example 4

[0179] Experiment on the synergistic effect of different ratios of fenpyraclostrobin and dithiopyracil and their control effect on grass weeds

[0180] 1 Test conditions

[0181] 1.1 Test targets

[0182] The test examples are detailed using Echinochloa crus-galli and Leptochloa chinensis, two of the most representative and difficult weeds to control in rice fields, as well as the newly dominant weed Digitaria sanguinalis. The seeds of the Echinochloa crus-galli, Leptochloa crus-galli, and Digitaria sanguinalis were all collected from rice fields.

[0183] Use the potting method. Use 140×140mm plastic pots placed on trays filled with air-dried, sieved topsoil collected from the field. Add water directly to the top of the pots to maintain saturated soil moisture initially. Select seeds of Echinochloa, Leptochloa chinensis, and Digitaria with plump, uniform seeds. Germinate in a 30°C incubator (dark). Place newly white weed seeds evenly on the soil surface and cover with 0.5cm of soil. Keep the soil moist after sowing and cultivate in a controlled sunlight greenhouse.

[0184] 1.2 Culture conditions

[0185] The growth was carried out in a controlled sunlight greenhouse with a night temperature of 15-20°C, a day temperature of 25-30°C, natural light, and a relative humidity of 60%-75%. The soil type was loam with an organic matter content of 1.8% and a pH of 7.1.

[0186] 1.3 Instruments and Equipment

[0187] ASS-4 automatic quantitative spray system, GA110 1 / 10,000 electronic balance;

[0188] ZDR2000 intelligent data logger, MLR-352H plant incubator.

[0189] 2. Experimental Design

[0190] 2.1 Test Agents

[0191] The 95% mass concentration of triazosulfuron technical and the 98% mass concentration of dithiopyralid technical are both prepared in acetone and diluted with a 0.1% Tween 80 aqueous solution. Dilute immediately before use.

[0192] 2.2 Experimental treatment

[0193] 2.2.1 Dose setting

[0194] Based on the respective activities of the two agents and the characteristics of laboratory testing, different mixed combinations and single-dose technical agents were set up based on the initial laboratory test. The highest formulation of fenpyroxene was 14.4g ai / hm 2 , the dilution was performed in a multiple of 2, and the final dose settings were 14.4, 7.20, 3.60, 1.80 and 0.90 g ai / hm 2At the same time, water without drug and containing the same emulsifier was used as blank control; the highest dithiopyr was 1800g ai / hm 2 , the dilution was performed in a multiple of 2, and the final dose was set as 1800, 900, 450, 225 and 112.5 g ai / hm 2 At the same time, water without the drug and containing the same emulsifier was used as a blank control. Six doses of the two drugs were combined in pairs, for a total of 36 combinations, and then the indoor bioassay was carried out.

[0195] 2.2.2 Experimental Replication

[0196] Each treatment was replicated 4 times, with 3 pots per treatment and 10 weed seeds sown in each pot, for a total of 30 plants per treatment.

[0197] 3. Handling Methods

[0198] 3.1 Processing time and frequency

[0199] The test was conducted with one application of the drug. When the Echinochloa weeds reached the 4-leaf stage, the seedlings were thinned out to keep 8 weeds in each pot, and 24 weeds were retained for each treatment.

[0200] 3.2 Instruments and medication methods

[0201] Weeds were evenly distributed on a platform and sprayed using an ASS-4 automatic quantitative spray system equipped with a TeeJet XR8002VS fan nozzle at a spray rate of 450 L / ha and a spray pressure of 0.275 MPa. After 30 seconds of spraying, the spray tower door was opened and the nutrient pots were removed. The air valve was then opened and 100 mL of clean water was sprayed to clean the spray pipe.

[0202] 4. Test methods

[0203] The test was carried out in accordance with the Guidelines for Indoor Bioassay Tests of Pesticides - NY / T1155.9-2008 and NY / T1155.7-2006.

[0204] 5. Data investigation and statistical analysis

[0205] 5.1 Survey Methods

[0206] Because the experimental concentration gradients were inconsistent, weeds at low concentrations were not completely killed. Therefore, the absolute number survey method was used to investigate the fresh weight of weeds. Surviving weed seedlings were cut along the soil surface with scissors and the fresh weight of the weeds was weighed using an analytical balance.

[0207] 5.2 Survey duration and frequency

[0208] The survey was conducted 15 days after treatment, for a total of 1 survey.

[0209] 5.3 Data Statistical Analysis

[0210] The logarithm of the dosage and the probability of the control effect were analyzed by DPS statistical software, and the herbicide dosage that inhibited the growth of weeds by 90% (GR 90 The isobologram method was used to determine whether the mixture had a synergistic effect. There was no significant difference between the two replicates.

[0211] The results of the indoor tests are shown in Tables 10-12 below. The results of using the isoequivalent line method to determine whether the mixture has a synergistic effect are as follows: Figure 10-12 As shown:

[0212] Table 10 Synergistic effect of combination of fenpyraclostrobin and dithiopyralid in controlling Echinochloa weeds

[0213]

[0214] From Table 10 and Figure 10 It can be seen that compared with the single doses of the two, the combination of fenpyrazone and dithiopyracil has a significant synergistic effect on the fresh weight inhibition of Echinochloa spp. All points are located below the isobol line, indicating that each formulation ratio in the example has a synergistic effect, and the maximum synergistic point is (5.08, 90). When the dosage of fenpyrazone and dithiopyracil reaches 14.4 + 450g ai / hm 2 When the amount of the compound is less than 1%, the fresh weight control effect on Echinochloa weeds can reach 100%.

[0215] Table 11 Synergistic effect of combination of fenpyraclostrobin and dithiopyralid on controlling Leptochloa chinensis

[0216]

[0217]

[0218] From Table 11 and Figure 11 It can be seen that compared with the single doses of the two, the combination of fenpyraclostrobin and dithiopyr has a significant synergistic effect on the fresh weight inhibition of Leptochloa chinensis. All points are located below the isobol line, indicating that each formulation ratio in the example has a synergistic effect, and the maximum synergistic point is (4.60, 90). When the dosage of fenpyraclostrobin and dithiopyr reaches 14.4+450g ai / hm 2 When used, the fresh weight protection effect on Leptochloa chinensis can reach 100%.

[0219] Table 12 Synergistic effect of combination of fenpyraclostrobin and dithiopyralid in controlling crabgrass

[0220]

[0221] From Table 12 and Figure 12It can be seen that compared with the single doses of the two, the combination of fenpyrazone and dithiopyr has a significant synergistic effect on the fresh weight inhibition of crabgrass. All points are located below the isobol line, indicating that each formulation ratio in the example has a synergistic effect, and the maximum synergistic point is (4.53, 90). When the dosage of fenpyrazone and dithiopyr reaches 14.4+450g ai / hm 2 When the fresh weight of crabgrass is controlled, the control effect can reach 100%.

[0222] Taking into account the comprehensive control effect, reduction requirements and cost, the ratio of the two is set at 1:20.

[0223] Example 4

[0224] According to the ratios in Examples 1-3, benzathine was added to triazosulfuron, quinpyraclostrobin, pyrazosulfuron-butyl and dithiopyracil in ratios of 1:60, 1:40, 1:36 and 1:20, respectively, to prepare corresponding preparations. The specific formula ratios are as follows.

[0225] 30.5% benzathine-triazole EC: Weigh 0.5% benzathine, 30% triazole, 4% sodium lauryl sulfate, and 3% alkylphenol polyoxyethylene ether, respectively, by weight, and make up to 100% with solvent naphtha. The recommended field dosage is 300-500 mL per hectare, which is 91.5-157.5 g ai / hm² of active ingredient. 2 .

[0226] 41% benzathine-methyl / pyraclostrobin SC: Weigh 1% benzathine-methyl, 40% pyraclostrobin, 4% polyoxyethylene sorbitan fatty acid ketone, 3% alkylphenol formaldehyde resin polyoxyethylene ether, and 1% ethylene glycol, respectively, to 100% by weight. The recommended field dosage is 150-250 mL / hectare, equivalent to 61.5-102.5 g ai / hm2 of active ingredient. 2 .

[0227] 37% benzathine-pyrazosulfuron-methyl emulsion in water: Weigh 1% benzathine-pyrazosulfuron, 36% pyrazosulfuron-methyl, 4% xylene, 3% calcium dodecylbenzenesulfonate, 4% sodium dioctyl sulfosuccinate, and 2% glycerol, respectively, by weight. Make up to 100% with water. The recommended dosage is 300-500 mL per hectare, equivalent to 111.1-185 g ai / hm² of active ingredient. 2 .

[0228] 42% fenpyrazone-disulfuron suspension concentrate: Weigh 2% fenpyrazone, 40% disulfuron, 5% polyoxyethylene sorbitan fatty acid ketone, 4% alkylphenol formaldehyde resin polyoxyethylene ether, and 1% ethylene glycol, respectively, by weight. Make up to 100% with water. The recommended field dosage is 600-1000 mL / hectare, equivalent to 252-420 g ai / hm2 of active ingredient. 2 .

[0229] Safety evaluation of four groups of synergistic compositions on rice

[0230] Using 30.5% benzathine·tripyrasulfuron emulsifiable concentrate, 41% benzathine·pyraclostrobin suspension concentrate, 37% benzathine·pyrazosulfuron-ethyl emulsifiable concentrate and 42% benzathine·disulfuron suspension concentrate as test agents, the safety of the preparations at different doses on rice was determined, in order to provide guidance for the field application of the four synergistic combinations.

[0231] 1 Test conditions

[0232] 1.1 Test targets

[0233] Japonica rice: Nanjing 46; Indica rice: August Fragrance.

[0234] Use the potting method. Use 140×140mm plastic nutrient pots placed on a tray and filled with air-dried, sieved topsoil collected from the field. Add water directly to the top of the nutrient pots to keep the soil moisture at saturation initially. Select rice seeds with plump, uniform grains and germinate them in a 30°C incubator (dark). Place the newly whitened rice seeds evenly on the soil surface and cover with 0.5cm of soil. Keep the soil moist after sowing and cultivate in a controlled sunlight greenhouse.

[0235] 1.2 Culture conditions

[0236] The growth was carried out in a controlled sunlight greenhouse with a night temperature of 15-20°C, a day temperature of 25-30°C, natural light, and a relative humidity of 60%-75%. The soil type was loam with an organic matter content of 1.8% and a pH of 7.1.

[0237] 1.3 Instruments and Equipment

[0238] ASS-4 automatic quantitative spray system, GA110 1 / 10,000 electronic balance;

[0239] ZDR2000 intelligent data logger, MLR-352H plant incubator.

[0240] 2. Experimental Design

[0241] 2.1 Test Agents

[0242] 30.5% benzathone·triazole sulfone emulsifiable concentrate, 41% benzathone·pyraclostrobin suspension concentrate, 37% benzathone·pyraclostrobin aqueous emulsion and 42% benzathone·disulfuron suspension concentrate.

[0243] 2.2 Experimental treatment

[0244] 2.2.1 Dose setting

[0245] According to the respective activities of the four agents and the characteristics of indoor determination, on the basis of the initial indoor test, four treatments of high, medium, low and medium dose were set up, and a clear water control was set up for indoor biological determination.

[0246] 2.2.2 Experimental repetition

[0247] Three pots were used for each treatment, and 10 weed seeds were sown in each pot, for a total of 30 plants per treatment.

[0248] 3. Handling Methods

[0249] 3.1 Processing time and frequency

[0250] The experiment used the drug once. After the rice grew to 3-4 leaves, the seedlings were thinned out, and 8 rice plants were kept in each pot, and 24 plants were retained for each treatment. The experiment was repeated twice.

[0251] 3.2 Instruments and medication methods

[0252] The rice plants were evenly distributed on a platform and sprayed using an ASS-4 automatic quantitative spray system with a TeeJet XR8002VS fan nozzle at a spray rate of 450 L / ha and a spray pressure of 0.275 MPa. After 30 seconds of spraying, the spray tower door was opened and the nutrient pots were removed. The air valve was then opened and 100 mL of clean water was sprayed to clean the spray pipe.

[0253] 4. Test methods

[0254] The test was carried out in accordance with the Guidelines for Indoor Bioassay Tests of Pesticides - NY / T1155.9-2008 and NY / T1155.7-2006.

[0255] 5. Data investigation and statistical analysis

[0256] 5.1 Survey Methods

[0257] The absolute number survey method was used to determine the fresh weight of rice. Surviving weed seedlings were cut along the soil surface with scissors and the fresh weight of rice was measured using an analytical balance.

[0258] 5.2 Survey duration and frequency

[0259] The survey was conducted 15 days after treatment, for a total of 1 survey.

[0260] 5.3 Data Statistical Analysis

[0261] The fresh weight data were analyzed for significant differences using DPS (P=0.05) to determine the safety of the synergistic composition to rice. There was no significant difference between the two replicates.

[0262] The indoor test results are shown in Tables 13-16:

[0263] Table 13 Safety of 30.5% benzathine-triazole EC on rice

[0264] <![CDATA[Processing (ga.i. / hm 2 )]]> Nanjing 46 fresh weight (g) August Fragrance Fresh Weight (g) 0 7.52a 7.01a 91.5 7.51a 6.97a 122 7.19a 6.52ab 152.5 7.02a 6.13b 244 7.00a 5.28c

[0265] As shown in Table 13, the fresh weight of both rice varieties gradually decreased with increasing herbicide dosage. However, there were no significant differences between the japonica rice treatments and the plain water control, indicating that 30.5% benzathine·tripyram sulfone EC is safe for japonica rice within the experimental range. However, the fresh weight of indica rice at high and twice the medium dose was significantly lower than that of the plain water control, while the fresh weight at the medium and low doses was not significantly different from that of the plain water control. This indicates that higher doses of 30.5% benzathine·tripyram sulfone EC are unsafe for indica rice, consistent with the poor safety profile of this herbicide for indica rice.

[0266] Table 14 Safety of 41% benzathine-methyl·quinoxaline suspension concentrate on rice

[0267] <![CDATA[Processing (ga.i. / hm 2 )]]> Nanjing 46 fresh weight (g) August Fragrance Fresh Weight (g) 0 7.52a 7.01a 61.5 7.30a 6.79a 82 7.08a 6.74a 102.5 6.97a 5.89b 164 6.77a 5.48b

[0268] As shown in Table 14, the fresh weight of both rice varieties gradually decreased with increasing herbicide dosage. However, there were no significant differences between the japonica rice treatments and the plain water control, indicating that the 41% benzathine-methyl·quinoxaline SC is safe for japonica rice within the experimental range. However, the fresh weight of indica rice at high and twice the medium dose was significantly lower than that of the plain water control, while the fresh weight at the medium and low doses was not significantly different from that of the plain water control. This indicates that higher doses of 41% benzathine-methyl·quinoxaline SC are unsafe for indica rice, consistent with the poor safety profile of this herbicide for indica rice.

[0269] Table 15 Safety of 37% benzathine-pyrazosulfuron-ethyl emulsion in water to rice

[0270] <![CDATA[Processing (ga.i. / hm 2 )]]> Nanjing 46 fresh weight (g) August Fragrance Fresh Weight (g) 0 7.52a 7.01a 111 7.51a 6.88a 148 7.49a 6.61a 185 7.32a 6.17b 296 7.09a 5.84b

[0271] As can be seen from Table 15, the fresh weight of both rice varieties gradually decreased with increasing dosage. However, there was no significant difference between the japonica rice treatments and the plain water control, indicating that the 37% benzathone·oxazolidinone pyrazosulfuron EW is safe for japonica rice within the experimental range. However, the fresh weight of indica rice at high and twice the medium dosage was significantly lower than that of the plain water control, while the fresh weight at medium and low dosages was not significantly different from that of the plain water control. This indicates that higher doses of 37% benzathone·oxazolidinone pyrazosulfuron are unsafe for indica rice and that attention should be paid to the timing and dosage of the application.

[0272] Table 16 Safety of 42% benzathine-pyraclostrobin SC to rice

[0273] <![CDATA[Processing (ga.i. / hm 2 )]]> Nanjing 46 fresh weight (g) August Fragrance Fresh Weight (g) 0 7.52a 7.01a 252 7.44a 6.96a 336 7.36a 6.45a 420 7.24a 6.01b 672 7.15a 5.33b

[0274] As shown in Table 16, the fresh weight of both rice varieties gradually decreased with increasing dosage. However, there were no significant differences between the japonica rice treatments and the plain water control, indicating that 42% benzathine·dithiopyr is safe for japonica rice within the experimental range. Meanwhile, the fresh weight of indica rice at high and twice the medium dosage was significantly lower than that of the plain water control, while the fresh weight at the medium and low dosages was not significantly different from that of the plain water control. This indicates that higher doses of 42% benzathine·dithiopyr are unsafe for indica rice and that caution should be exercised when using the drug, both at the time of use and at the dosage.

[0275] In summary, the four synergistic combinations are safe for japonica rice at the recommended doses, effectively controlling grass weeds while maintaining safety for japonica rice. However, at higher doses, the four synergistic combinations are unsafe for indica rice. In practical applications, specific rice varieties should be carefully considered to avoid phytotoxicity.

[0276] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A pesticide composition containing fenpyrazone, characterized in that: The invention comprises a first component and a second component, wherein the first component is fenpyrad, and the second component comprises triazosulfuron, quinpyraclostrobin or pyrazosulfuron-methyl; and the mass ratio of the first component to the second component is 1:0.5-100.

2. Use of the pesticide composition according to claim 1 in pesticides.

3. A pesticide preparation containing fenpyrazone, characterized in that: The pesticide composition according to claim 1, comprising adjuvants and an effective dose.

4. The pesticide formulation according to claim 3, characterized in that The mass of the pesticide composition is 8%-72% of the total mass of the pesticide formulation.

5. The pesticide formulation according to claim 4, characterized in that The mass of the pesticide composition is 12%-54% of the total mass of the pesticide formulation.

6. The pesticide formulation according to any one of claims 3 to 5, characterized in that The dosage form of the pesticide preparation includes wettable powder, emulsifiable concentrate, suspension concentrate or aqueous emulsion.

7. Use of the pesticide composition according to claim 1 or the pesticide formulation according to any one of claims 3 to 6 in controlling weeds.

8. The use according to claim 7, characterized in that The weeds include weeds in rice fields; the types of weeds include grass weeds.

9. The use according to claim 8, characterized in that The grass weeds include one or more of Echinochloa spp., Leptochloa chinensis, Digitaria sanguinalis, weedy rice and false rice.

10. A method for controlling weeds in rice fields, characterized in that: include: After the rice reaches the 4-leaf stage, spraying the pesticide formulation according to any one of claims 3 to 6 on the stems and leaves of the rice; Based on the dosage of the pesticide composition in the pesticide preparation, the dosage of the pesticide preparation is 61.5-296 g a.i. / hm 2 .

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