A traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors

Abstract

The present invention belongs to the field of preparation of longan ginseng peptide probiotic powder, and particularly relates to a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors. Ginseng and American ginseng are crushed and powdered, vacuum concentrated to make ginseng peptide concentrate, and mixed with dried longan, artemisia annua, ashwagandha, knotweed, tripterygium wilfordii, cordyceps sinensis, and composite probiotic agents in a fermenter for full fermentation. The effective ingredient content is high and is conducive to absorption, while clearing away heat and promoting body fluid without toxic side effects; finally, the traditional Chinese medicine fermented longan ginseng peptide probiotic powder prepared by vacuum freeze drying has minimal biological activity damage, completely retains the active ingredients for treating tumors, and is convenient for long-term storage. After liver cancer mice take it, the tumor weight and volume are effectively suppressed, the liver function is less damaged, and the immune function of mice is protected, and the macrophage phagocytosis rate of mice reaches 79.6%.

Description

Technical Field

[0001] The invention belongs to the field of preparation of longan ginseng peptide probiotic powder, and specifically refers to a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors. Background Art

[0002] Tumors are formed by the proliferation of local tissue cells under the action of various tumorigenic factors. Tumor cells have metabolic changes different from normal cells. At the same time, tumor cells can undergo metabolism between glycolysis and oxidative phosphorylation.

[0003] Tumors can harm health, damage body tissues, cause painful infections, and lead to decreased organ function. As tumors grow, they can compress or block surrounding organs, and even endanger life. The body can develop cachexia, leading to extreme weight loss, and death due to the failure of various system functions.

[0004] At present, the existing drug preparation technology for treating tumors mainly has the following problems: First, there is a lack of fundamental metabolic differences between tumor cells and normal cells. Drugs for treating tumors cannot completely avoid damage to normal tissues, causing bone marrow suppression, affecting bone marrow hematopoietic function, and causing leukopenia; Second, it stimulates the gastrointestinal tract, causing side effects such as nausea, diarrhea, abdominal pain, and hair loss, and stimulates the nervous system to cause dizziness, insomnia, fatigue and other discomfort symptoms; Third, therapeutic anti-tumor drugs need to be metabolized by the liver. Continuous use will cause certain damage to liver cells, causing liver fibrosis, damaging liver cell function, and elevated transaminases; Fourth, it stimulates the body's immune system, leading to immune thyroiditis, hyperthyroidism, immune dermatitis rash itching, decreased lymphocyte counts and other manifestations. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors and a preparation method thereof. In order to solve the problems that the existing tumor treating agents damage normal tissues of the body, have strong side effects, damage the gastrointestinal tract and liver, and endanger the immune system, the present invention uses ginseng peptides, Chinese herbal medicines and probiotic agents to effectively inhibit the proliferation and migration of tumor cells and promote the apoptosis of tumor cells. The prepared traditional Chinese medicine fermented longan ginseng peptide probiotic powder has no toxic side effects, protects the gastrointestinal tract and is well absorbed.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows: the present invention proposes a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors, comprising the following components in parts by weight:

[0007] 180-230 parts of ginseng peptide concentrate, 100-150 parts of dried longan, 80-110 parts of artemisia annua, 60-90 parts of ashwagandha, 60-85 parts of knotweed, 50-70 parts of tripterygium wilfordii, 20-30 parts of cordyceps sinensis, and 45-70 parts of compound probiotic agent.

[0008] Preferably, the composite probiotic agent mainly comprises: Bifidobacterium, Clostridium butyricum, Akkermansia muciniphila, and Lactobacillus reuteri, and the mixing ratio by weight is 1-4:2-3:1-2:1.

[0009] Furthermore, the Bifidobacterium was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.5090; the Clostridium butyricum was purchased from the China General Microorganism Culture Collection Center with a collection number of CGMCC1.5205; the Akkermansia muciniphila was purchased from the China Industrial Microorganism Culture Collection Center with a collection number of CICC24917; and the Lactobacillus reuteri was purchased from the Guangdong Provincial Microorganism Culture Collection Center with a collection number of GDMCC1.1805.

[0010] Preferably, the tumor is a liver cancer tumor.

[0011] Preferably, the method for preparing the ginseng peptide concentrate comprises the following steps:

[0012] S1, weighing 100-150 parts by weight of ginseng roots and 30-40 parts of American ginseng roots, putting them into a 2-3.5 kW grinder for grinding at a temperature of 20-25° C. for 1-1.5 h to obtain mixed ginseng fragments;

[0013] S2, putting the mixed ginseng pieces prepared in S1 into a powder grinder with a power of 2-2.5 kW, grinding them at a temperature of 23-26° C. for 1.5-2.5 h to obtain mixed ginseng powder;

[0014] S3, filtering the mixed ginseng powder prepared in S2 through a 200-300 mesh filter sieve to obtain fine ginseng powder;

[0015] S4, put the fine ginseng powder prepared in S3 into a stirring tank, add 300 parts by weight of ultrapure water, stir to dissolve, the stirring speed is 150-200 r / min, the stirring temperature is 30-35°C, and the stirring time is 2-3.5 h to obtain a ginseng powder solution;

[0016] S5, putting the ginseng powder solution prepared in S4 into an enzymolysis tank, adjusting the pH to 5.0 with 5 mol / L hydrochloric acid and heating to 50-60°C, adding 60-100 parts by weight of papain and 50-80 parts by weight of glucan exoenase, stirring for enzymolysis, the enzymolysis speed is 150-170 r / min, the enzymolysis time is 4-6 h, and a ginseng peptide mixed solution is obtained;

[0017] S6, placing the ginseng peptide mixed solution prepared in S5 into a centrifuge with a power of 12-16 kW, centrifuging at a centrifugal temperature of 0°C, a centrifugal speed of 2000-2200 r / min, and a centrifugal time of 20-30 min, taking the supernatant to obtain a ginseng peptide solution;

[0018] S7. Place the ginseng peptide solution prepared in S6 into a vacuum concentrator for vacuum concentration at a vacuum degree of -0.08-0 MPa, a concentration temperature of 30-35°C, and a concentration time of 2-3 h to obtain a ginseng peptide concentrate.

[0019] The present invention also proposes a method for preparing a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors, which specifically comprises the following steps:

[0020] Step 1, weighing appropriate weight portions of dried longan, artemisia annua, ashwagandha, knotweed, tripterygium wilfordii, and cordyceps sinensis and putting them into a powder grinder to grind them into a mixed Chinese herbal medicine powder;

[0021] Step 2: Weigh appropriate weight portions of ginseng peptide concentrate and composite probiotic agent into a stirring tank, add the mixed Chinese herbal medicine powder prepared in step 1, and stir evenly to obtain a pre-fermentation mixture;

[0022] Step 3, placing the pre-fermentation mixture prepared in step 2 into a fermentation tank for fermentation to obtain a Chinese medicine fermented longan ginseng peptide probiotic mixture;

[0023] Step 4: Put the Chinese medicine fermented longan ginseng peptide probiotic mixture prepared in step 3 into a vacuum freeze dryer, freeze it and then heat it to dry it, so as to obtain the Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors.

[0024] Preferably, in step 1, the power of the powdering machine is 1.8-2.5 kW, the powdering temperature is 20-23° C., and the powdering time is 1-1.6 h.

[0025] Preferably, in step 2, the power of the stirring tank is 10-13.5 kW, the stirring speed is 200-260 r / min, the stirring temperature is 26-28° C., and the stirring time is 1.5-2.3 h.

[0026] Preferably, in step three, the power of the fermentation tank is 20-25 kW, the fermentation temperature is 35-39° C., the fermentation time is 6-8 h, and the fermentation tank speed is 180-230 r / min.

[0027] Preferably, in step 4, the power of the vacuum freeze dryer is 6-7.5 kW, the vacuum degree is 10-18 kPa, the freezing temperature is -40°C, the freezing time is 2-2.5 h, the heating temperature is 8-15°C, and the drying time is 12-24 h.

[0028] The beneficial effects achieved by the present invention are as follows:

[0029] The invention proposes to mix ginseng and American ginseng to prepare ginseng peptide concentrate. Ginsenoside in ginseng peptide is a natural anti-cancer active ingredient, which can act on tumor cell mitochondria to release cell death signal molecules to activate cysteine ​​protease and accelerate tumor cell apoptosis. The addition of American ginseng makes the prepared traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors have the effect of clearing heat and promoting body fluid, and will not cause dry mouth and tongue after taking it; vitamin K in longan can accelerate the death of tumor cells, double artemisinin in artemisia annua controls tumor protein phosphorylation by inhibiting translation, reduces the activation of cell division cycle proteins, and inhibits the invasion and migration of tumor cells; withaferin A in ashwagandha can block tumor cells in the mitosis preparation period, thereby inhibiting the proliferation of tumor cells and inducing their apoptosis; rhubarb in polygonum cuspidatum can enhance the toxicity of tumor cells themselves by increasing the level of active oxygen in tumor cells; triptolide in tripterygium wilfordii can enhance the growth inhibitory effect of fluorouracil on tumor cells; cordyceps sinensis contains cordycepin to enhance the expression of autophagy-related marker proteins, and inhibits tumor growth by inducing cell autophagy. Tumor cell proliferation; Bifidobacterium can increase the production of tumor necrosis factor and interleukin, activate T cells, make them produce in large quantities, improve the body's immune function, protect the body, and inhibit the growth of tumor cells. Clostridium butyricum reduces the secretion of inflammatory factors, promotes tumor cell apoptosis, and regulates the immune system. Akkermansia muciniphila can induce the body's antigenic T cell response and promote the production of IgG1 antibodies. Lactobacillus reuteri can stimulate killer T cells by secreting indole-3-formaldehyde and inhibit tumor size. Bifidobacterium and Clostridium butyricum can also secrete amylase, pectinase, protease, and xylanase. After fermentation, the active ingredients in the mixed Chinese herbal powder are more conducive to absorption and increase the speed of drug efficacy. The addition of probiotics can also effectively regulate intestinal microbial flora, promote nutrient absorption, enhance immunity, relieve inflammatory reactions, and synergize with the mixed Chinese herbal powder to improve the effect of treating tumors. The biological activity and active ingredients of the Chinese herbal fermented longan ginseng peptide probiotic powder after vacuum freeze-drying are minimally damaged, and the active ingredients for treating tumors are retained, which is convenient for long-term storage. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following will be described in a clear and understandable manner in conjunction with the accompanying drawings. Obviously, the drawings described below are only for the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0031] Figure 1 This is a graph showing the weight of subcutaneous tumors on the back of mice described in Experimental Example 1 of the present invention;

[0032] Figure 2 This is a graph showing the volume of subcutaneous tumors on the back of mice described in Experimental Example 1 of the present invention;

[0033] Figure 3 This is a graph showing the results of serum transaminase activity in mice described in Experimental Example 1 of the present invention;

[0034] Figure 4 This is a graph showing the phagocytic rate of mouse macrophages described in Experimental Example 2 of the present invention;

[0035] Figure 5 This is a graph showing the results of the mouse macrophage phagocytic index described in Experimental Example 2 of the present invention. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0037] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0038] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials and test strains used in the following examples are purchased from commercial channels unless otherwise specified.

[0039] Example 1: This example provides a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors and a preparation method thereof. The traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors comprises the following components in parts by weight:

[0040] 180 parts of ginseng peptide concentrate, 100 parts of dried longan, 80 parts of artemisia annua, 60 parts of ashwagandha, 60 parts of knotweed, 50 parts of tripterygium wilfordii, 20 parts of cordyceps sinensis, and 45 parts of compound probiotic agent.

[0041] The composite probiotic bacterial agent mainly comprises: bifidobacterium, Clostridium butyricum, Akkermansia muciniphila, and Lactobacillus reuteri, and the mixing ratio by weight is 1:2:1:1.

[0042] The Bifidobacterium was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC1.5090; the Clostridium butyricum was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC1.5205; the Akkermansia muciniphila was purchased from the China Industrial Microbiological Culture Collection Center with a collection number of CICC24917; and the Lactobacillus reuteri was purchased from the Guangdong Provincial Microbiological Culture Collection Center with a collection number of GDMCC1.1805.

[0043] The tumor is a liver cancer tumor.

[0044] The preparation method of the ginseng peptide concentrated solution specifically comprises the following steps:

[0045] S1. Weigh 100 parts by weight of ginseng roots and 30 parts of American ginseng roots and put them into a 2 kW grinder for grinding at a temperature of 20° C. for 1 h to obtain mixed ginseng pieces;

[0046] S2, putting the mixed ginseng pieces prepared in S1 into a powder grinder with a power of 2 kW, grinding them at a powdering temperature of 23° C. for 1.5 h to obtain mixed ginseng powder;

[0047] S3, filtering the mixed ginseng powder prepared in S2 through a 200-mesh filter sieve to obtain fine ginseng powder;

[0048] S4, put the fine ginseng powder prepared in S3 into a stirring tank, add 300 parts by weight of ultrapure water, stir to dissolve, the stirring speed is 150 r / min, the stirring temperature is 30°C, and the stirring time is 2 h to obtain a ginseng powder solution;

[0049] S5, putting the ginseng powder solution prepared in S4 into an enzymatic hydrolysis tank, adjusting the pH to 5.0 with 5 mol / L hydrochloric acid and heating to 50°C, adding 60 parts by weight of papain and 50 parts by weight of glucan exoenzyme, stirring and enzymolysis, the enzymatic hydrolysis speed is 150 r / min, the enzymatic hydrolysis time is 4 h, and a ginseng peptide mixed solution is obtained;

[0050] S6, placing the ginseng peptide mixed solution prepared in S5 into a centrifuge with a power of 12 kW, centrifuging at a centrifugal temperature of 0°C, a centrifugal speed of 2000 r / min, and a centrifugal time of 20 min, taking the supernatant to obtain a ginseng peptide solution;

[0051] S7. Place the ginseng peptide solution prepared in S6 into a vacuum concentrator for vacuum concentration at a vacuum degree of -0.08 MPa, a concentration temperature of 30°C, and a concentration time of 2 h to obtain a ginseng peptide concentrate.

[0052] This embodiment also provides a method for preparing a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors, which specifically comprises the following steps:

[0053] Step 1, weigh appropriate weight portions of dried longan, artemisia annua, ashwagandha, knotweed, tripterygium wilfordii, and cordyceps sinensis and put them into a powder grinder for powdering. The power of the powder grinder is 1.8 kW, the powdering temperature is 20° C., and the powdering time is 1 h to obtain mixed Chinese herbal medicine powder;

[0054] Step 2: Weigh appropriate weight portions of ginseng peptide concentrate and composite probiotic agent into a stirring tank, add the mixed Chinese herbal medicine powder prepared in step 1, the stirring tank power is 10 kW, the stirring speed is 200 r / min, the stirring temperature is 26°C, the stirring time is 1.5 h, stir evenly to obtain a pre-fermentation mixture;

[0055] Step 3, placing the pre-fermentation mixture prepared in step 2 into a fermentation tank, the fermentation tank power is 20 kW, the fermentation temperature is 35° C., the fermentation time is 6 h, the fermentation tank speed is 180 r / min, and fermentation is performed to obtain a Chinese medicine fermented longan ginseng peptide probiotic mixture;

[0056] Step 4: Place the Chinese medicine fermented longan ginseng peptide probiotic mixture prepared in step 3 into a vacuum freeze dryer, freeze it and then heat it to dry it. The power of the vacuum freeze dryer is 6 kW, the vacuum degree is 10 kPa, the freezing temperature is -40°C, the freezing time is 2 h, the heating temperature is 8°C, and the drying time is 12 h to obtain the Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors.

[0057] Example 2: This example provides a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors and a preparation method thereof. The traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors comprises the following components in parts by weight:

[0058] 200 parts of ginseng peptide concentrate, 130 parts of dried longan, 100 parts of artemisia annua, 80 parts of ashwagandha, 75 parts of knotweed, 60 parts of tripterygium wilfordii, 25 parts of cordyceps sinensis, and 60 parts of compound probiotic agent.

[0059] The composite probiotic bacterial agent mainly comprises: bifidobacterium, clostridium butyricum, Akkermansia muciniphila, and Lactobacillus reuteri, and the mixing ratio by weight is 2:2.5:1.5:1.

[0060] The Bifidobacterium was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC1.5090; the Clostridium butyricum was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC1.5205; the Akkermansia muciniphila was purchased from the China Industrial Microbiological Culture Collection Center with a collection number of CICC24917; and the Lactobacillus reuteri was purchased from the Guangdong Provincial Microbiological Culture Collection Center with a collection number of GDMCC1.1805.

[0061] The tumor is a liver cancer tumor.

[0062] The preparation method of the ginseng peptide concentrated solution specifically comprises the following steps:

[0063] S1, weighing 130 parts by weight of ginseng roots and 35 parts of American ginseng roots, putting them into a 2.5 kW grinder for grinding at a temperature of 23° C. for 1.3 h to obtain mixed ginseng pieces;

[0064] S2, putting the mixed ginseng pieces prepared in S1 into a 2.3 kW powder grinder, the powder grinding temperature is 25°C, the powder grinding time is 2 h, and the mixed ginseng powder is obtained;

[0065] S3, filtering the mixed ginseng powder prepared in S2 through a 200-mesh filter sieve to obtain fine ginseng powder;

[0066] S4, put the fine ginseng powder prepared in S3 into a stirring tank, add 300 parts by weight of ultrapure water, stir to dissolve, the stirring speed is 180 r / min, the stirring temperature is 33°C, and the stirring time is 3 h to obtain a ginseng powder solution;

[0067] S5, putting the ginseng powder solution prepared in S4 into an enzymatic hydrolysis tank, adjusting the pH to 5.0 with 5 mol / L hydrochloric acid and heating to 55°C, adding 80 parts by weight of papain and 70 parts by weight of glucan exoenzyme, stirring and enzymolysis, the enzymatic hydrolysis speed is 160 r / min, the enzymatic hydrolysis time is 5 h, and a ginseng peptide mixed solution is obtained;

[0068] S6, placing the ginseng peptide mixed solution prepared in S5 into a centrifuge with a power of 14 kW, centrifuging at a centrifugal temperature of 0°C, a centrifugal speed of 2100 r / min, and a centrifugal time of 25 min, taking the supernatant to obtain a ginseng peptide solution;

[0069] S7. Place the ginseng peptide solution prepared in S6 into a vacuum concentrator for vacuum concentration at a vacuum degree of -0.04 MPa, a concentration temperature of 33°C, and a concentration time of 2.5 h to obtain a ginseng peptide concentrate.

[0070] This embodiment also provides a method for preparing a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors, which specifically comprises the following steps:

[0071] Step 1, weigh appropriate weight portions of dried longan, artemisia annua, ashwagandha, knotweed, tripterygium wilfordii, and cordyceps sinensis and put them into a powder grinder for powdering. The power of the powder grinder is 2.3 kW, the powdering temperature is 22° C., and the powdering time is 1.3 h to obtain mixed Chinese herbal medicine powder;

[0072] Step 2: Weigh appropriate weight portions of ginseng peptide concentrate and composite probiotic agent into a stirring tank, add the mixed Chinese herbal medicine powder prepared in step 1, the stirring tank power is 12 kW, the stirring speed is 230 r / min, the stirring temperature is 27°C, the stirring time is 2 h, stir evenly to obtain a pre-fermentation mixture;

[0073] Step 3, placing the pre-fermentation mixture prepared in step 2 into a fermentation tank, the fermentation tank power is 23 kW, the fermentation temperature is 37° C., the fermentation time is 7 h, the fermentation tank speed is 200 r / min, and fermentation is performed to obtain a Chinese medicine fermented longan ginseng peptide probiotic mixture;

[0074] Step 4: Place the Chinese medicine fermented longan ginseng peptide probiotic mixture prepared in step 3 into a vacuum freeze dryer, freeze it and then heat it to dry it. The power of the vacuum freeze dryer is 7 kW, the vacuum degree is 15 kPa, the freezing temperature is -40°C, the freezing time is 2.3 h, the heating temperature is 12°C, and the drying time is 18 h to obtain the Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors.

[0075] Example 3: This example provides a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors and a preparation method thereof. The traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors comprises the following components in parts by weight:

[0076] 230 parts of ginseng peptide concentrate, 150 parts of dried longan, 110 parts of artemisia annua, 90 parts of ashwagandha, 85 parts of knotweed, 70 parts of tripterygium wilfordii, 30 parts of cordyceps sinensis, and 70 parts of compound probiotic agent.

[0077] The composite probiotic bacterial agent mainly comprises: bifidobacterium, Clostridium butyricum, Akkermansia muciniphila, and Lactobacillus reuteri, and the mixing ratio by weight is 4:3:2:1.

[0078] The Bifidobacterium was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC1.5090; the Clostridium butyricum was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC1.5205; the Akkermansia muciniphila was purchased from the China Industrial Microbiological Culture Collection Center with a collection number of CICC24917; and the Lactobacillus reuteri was purchased from the Guangdong Provincial Microbiological Culture Collection Center with a collection number of GDMCC1.1805.

[0079] The tumor is a liver cancer tumor.

[0080] The preparation method of the ginseng peptide concentrated solution specifically comprises the following steps:

[0081] S1, weighing 150 parts by weight of ginseng roots and 40 parts of American ginseng roots, putting them into a 3.5 kW grinder for grinding at a temperature of 25° C. for 1.5 h to obtain mixed ginseng pieces;

[0082] S2, putting the mixed ginseng pieces prepared in S1 into a 2.5 kW powder grinder, the powder grinding temperature is 26°C, the powder grinding time is 2.5 h, and the mixed ginseng powder is obtained;

[0083] S3, filtering the mixed ginseng powder prepared in S2 through a 300-mesh filter sieve to obtain fine ginseng powder;

[0084] S4, put the fine ginseng powder prepared in S3 into a stirring tank, add 300 parts by weight of ultrapure water, stir to dissolve, the stirring speed is 200 r / min, the stirring temperature is 35°C, and the stirring time is 3.5 h to obtain a ginseng powder solution;

[0085] S5, putting the ginseng powder solution prepared in S4 into an enzymatic hydrolysis tank, adjusting the pH to 5.0 with 5 mol / L hydrochloric acid and heating to 60°C, adding 100 parts by weight of papain and 80 parts by weight of glucan exoenzyme, stirring and performing enzymatic hydrolysis at a speed of 170 r / min for 6 h to obtain a ginseng peptide mixed solution;

[0086] S6, placing the ginseng peptide mixed solution prepared in S5 into a centrifuge with a power of 16 kW, centrifuging at a centrifugal temperature of 0°C, a centrifugal speed of 2200 r / min, and a centrifugal time of 30 min, taking the supernatant to obtain a ginseng peptide solution;

[0087] S7. Place the ginseng peptide solution prepared in S6 into a vacuum concentrator for vacuum concentration with a vacuum degree of 0 MPa, a concentration temperature of 35°C, and a concentration time of 3 h to obtain a ginseng peptide concentrate.

[0088] This embodiment also provides a method for preparing a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors, which specifically comprises the following steps:

[0089] Step 1, weigh appropriate weight portions of dried longan, artemisia annua, ashwagandha, knotweed, tripterygium wilfordii, and cordyceps sinensis and put them into a powder grinder for powdering. The power of the powder grinder is 2.5 kW, the powdering temperature is 23° C., and the powdering time is 1.6 h to obtain mixed Chinese herbal medicine powder;

[0090] Step 2: Weigh appropriate weight portions of ginseng peptide concentrate and composite probiotic agent into a stirring tank, add the mixed Chinese herbal medicine powder prepared in step 1, the stirring tank power is 13.5 kW, the stirring speed is 260 r / min, the stirring temperature is 28°C, the stirring time is 2.3 h, stir evenly to obtain a pre-fermentation mixture;

[0091] Step 3, placing the pre-fermentation mixture prepared in step 2 into a fermentation tank, the fermentation tank power is 25 kW, the fermentation temperature is 39° C., the fermentation time is 8 h, the fermentation tank speed is 230 r / min, and fermentation is performed to obtain a Chinese medicine fermented longan ginseng peptide probiotic mixture;

[0092] Step 4: Place the Chinese medicine fermented longan ginseng peptide probiotic mixture prepared in step 3 into a vacuum freeze dryer, freeze it and then heat it to dry it. The power of the vacuum freeze dryer is 7.5 kW, the vacuum degree is 18 kPa, the freezing temperature is -40°C, the freezing time is 2.5 h, the heating temperature is 15°C, and the drying time is 24 h to obtain the Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors.

[0093] Example 4: This example provides a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors and a preparation method thereof. The traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors comprises the following components in parts by weight:

[0094] 190 parts of ginseng peptide concentrate, 120 parts of dried longan, 90 parts of artemisia annua, 70 parts of ashwagandha, 70 parts of knotweed, 55 parts of tripterygium wilfordii, 23 parts of cordyceps sinensis, and 55 parts of compound probiotic agent.

[0095] The composite probiotic agent mainly comprises: bifidobacterium, clostridium butyricum, Akkermansia muciniphila, and Lactobacillus reuteri, and the mixing ratio by weight is 1.5:2.2:1.3:1.

[0096] The Bifidobacterium was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC1.5090; the Clostridium butyricum was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC1.5205; the Akkermansia muciniphila was purchased from the China Industrial Microbiological Culture Collection Center with a collection number of CICC24917; and the Lactobacillus reuteri was purchased from the Guangdong Provincial Microbiological Culture Collection Center with a collection number of GDMCC1.1805.

[0097] The tumor is a liver cancer tumor.

[0098] The preparation method of the ginseng peptide concentrated solution specifically comprises the following steps:

[0099] S1, weighing 120 parts by weight of ginseng roots and 32 parts of American ginseng roots, putting them into a 2.3 kW grinder for grinding at a temperature of 22° C. for 1.2 h to obtain mixed ginseng pieces;

[0100] S2, putting the mixed ginseng pieces prepared in S1 into a 2.1 kW powder grinder, the powder grinding temperature is 24°C, the powder grinding time is 1.8 h, and the mixed ginseng powder is obtained;

[0101] S3, filtering the mixed ginseng powder prepared in S2 through a 200-mesh filter sieve to obtain fine ginseng powder;

[0102] S4, put the fine ginseng powder prepared in S3 into a stirring tank, add 300 parts by weight of ultrapure water, stir to dissolve, the stirring speed is 170 r / min, the stirring temperature is 32°C, and the stirring time is 2.8 h to obtain a ginseng powder solution;

[0103] S5, put the ginseng powder solution prepared in S4 into an enzymatic hydrolysis tank, adjust the pH to 5.0 with 5 mol / L hydrochloric acid and heat to 52°C, add 70 parts by weight of papain and 60 parts of exoglucanase, stir and perform enzymatic hydrolysis at a speed of 155 r / min for 4.6 h to obtain a ginseng peptide mixed solution;

[0104] S6, placing the ginseng peptide mixed solution prepared in S5 into a centrifuge with a power of 13 kW, centrifuging at a centrifugal temperature of 0°C, a centrifugal speed of 2050 r / min, and a centrifugal time of 23 min, taking the supernatant to obtain a ginseng peptide solution;

[0105] S7. Place the ginseng peptide solution prepared in S6 into a vacuum concentrator for vacuum concentration at a vacuum degree of -0.06 MPa, a concentration temperature of 2°C, and a concentration time of 2.2 h to obtain a ginseng peptide concentrate.

[0106] This embodiment also provides a method for preparing a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors, which specifically comprises the following steps:

[0107] Step 1, weigh appropriate weight portions of dried longan, artemisia annua, ashwagandha, knotweed, tripterygium wilfordii, and cordyceps sinensis and put them into a powder grinder for powdering. The power of the powder grinder is 2.2 kW, the powdering temperature is 21° C., and the powdering time is 1.2 h to obtain mixed Chinese herbal medicine powder;

[0108] Step 2: Weigh appropriate weight portions of ginseng peptide concentrate and composite probiotic agent into a stirring tank, add the mixed Chinese herbal medicine powder prepared in step 1, the stirring tank power is 11 kW, the stirring speed is 220 r / min, the stirring temperature is 27°C, the stirring time is 1.8 h, stir evenly to obtain a pre-fermentation mixture;

[0109] Step 3, placing the pre-fermentation mixture prepared in step 2 into a fermentation tank, the fermentation tank power is 22 kW, the fermentation temperature is 36° C., the fermentation time is 6.8 h, the fermentation tank speed is 190 r / min, and fermentation is performed to obtain a Chinese medicine fermented longan ginseng peptide probiotic mixture;

[0110] Step 4: Place the Chinese medicine fermented longan ginseng peptide probiotic mixture prepared in step 3 into a vacuum freeze dryer, freeze it and then heat it to dry it. The power of the vacuum freeze dryer is 6.5 kW, the vacuum degree is 13 kPa, the freezing temperature is -40°C, the freezing time is 2.1 h, the heating temperature is 10°C, and the drying time is 16 h to obtain the Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors.

[0111] Example 5: This example provides a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors and a preparation method thereof. The traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors comprises the following components in parts by weight:

[0112] 220 parts of ginseng peptide concentrate, 140 parts of dried longan, 105 parts of artemisia annua, 88 parts of ashwagandha, 80 parts of knotweed, 66 parts of tripterygium wilfordii, 28 parts of cordyceps sinensis, and 65 parts of compound probiotic agent.

[0113] The composite probiotic bacterial agent mainly comprises: bifidobacterium, Clostridium butyricum, Akkermansia muciniphila, and Lactobacillus reuteri, and the mixing ratio by weight is 3:2.8:1.7:1.

[0114] The Bifidobacterium was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC1.5090; the Clostridium butyricum was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC1.5205; the Akkermansia muciniphila was purchased from the China Industrial Microbiological Culture Collection Center with a collection number of CICC24917; and the Lactobacillus reuteri was purchased from the Guangdong Provincial Microbiological Culture Collection Center with a collection number of GDMCC1.1805.

[0115] The tumor is a liver cancer tumor.

[0116] The preparation method of the ginseng peptide concentrated solution specifically comprises the following steps:

[0117] S1, weighing 140 parts by weight of ginseng roots and 38 parts of American ginseng roots, putting them into a 3.2 kW grinder for grinding at a temperature of 24° C. for 1.4 h to obtain mixed ginseng pieces;

[0118] S2, putting the mixed ginseng pieces prepared in S1 into a 2.4 kW powder grinder, the powder grinding temperature is 25°C, the powder grinding time is 2.4 h, and the mixed ginseng powder is obtained;

[0119] S3, filtering the mixed ginseng powder prepared in S2 through a 300-mesh filter sieve to obtain fine ginseng powder;

[0120] S4, put the fine ginseng powder prepared in S3 into a stirring tank, add 300 parts by weight of ultrapure water, stir to dissolve, the stirring speed is 190 r / min, the stirring temperature is 34°C, and the stirring time is 3.3 h to obtain a ginseng powder solution;

[0121] S5, putting the ginseng powder solution prepared in S4 into an enzymatic hydrolysis tank, adjusting the pH to 5.0 with 5 mol / L hydrochloric acid and heating to 58°C, adding 90 parts by weight of papain and 75 parts by weight of glucan exoenase, stirring and enzymolysis, the enzymatic hydrolysis speed is 165 r / min, the enzymatic hydrolysis time is 5.6 h, and a ginseng peptide mixed solution is obtained;

[0122] S6, placing the ginseng peptide mixed solution prepared in S5 into a centrifuge with a power of 15 kW, centrifuging at a centrifugal temperature of 0°C, a centrifugal speed of 2150 r / min, and a centrifugal time of 28 min, taking the supernatant to obtain a ginseng peptide solution;

[0123] S7. Place the ginseng peptide solution prepared in S6 into a vacuum concentrator for vacuum concentration at a vacuum degree of -0.02 MPa, a concentration temperature of 34°C, and a concentration time of 2.8 h to obtain a ginseng peptide concentrate.

[0124] This embodiment also provides a method for preparing a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors, which specifically comprises the following steps:

[0125] Step 1, weigh appropriate weight portions of dried longan, artemisia annua, ashwagandha, knotweed, tripterygium wilfordii, and cordyceps sinensis and put them into a powder grinder for powdering. The power of the powder grinder is 2.4 kW, the powdering temperature is 22° C., and the powdering time is 1.5 h to obtain mixed Chinese herbal medicine powder;

[0126] Step 2: Weigh appropriate weight portions of ginseng peptide concentrate and composite probiotic agent into a stirring tank, add the mixed Chinese herbal medicine powder prepared in step 1, the stirring tank power is 13 kW, the stirring speed is 250 r / min, the stirring temperature is 27°C, the stirring time is 2.2 h, stir evenly to obtain a pre-fermentation mixture;

[0127] Step 3, placing the pre-fermentation mixture prepared in step 2 into a fermentation tank, the fermentation tank power is 24 kW, the fermentation temperature is 38° C., the fermentation time is 7.5 h, the fermentation tank speed is 220 r / min, and fermentation is performed to obtain a Chinese medicine fermented longan ginseng peptide probiotic mixture;

[0128] Step 4: Place the Chinese medicine fermented longan ginseng peptide probiotic mixture prepared in step 3 into a vacuum freeze dryer, freeze it and then heat it to dry it. The power of the vacuum freeze dryer is 7.2 kW, the vacuum degree is 17 kPa, the freezing temperature is -40°C, the freezing time is 2.4 h, the heating temperature is 14°C, and the drying time is 22 h to obtain the Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors.

[0129] Experimental Example 1: Experiment on inhibition of tumor growth and determination of biological response regulation effect in mice.

[0130] The experimental steps for determining the inhibitory effect and biological response regulation effect of a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors prepared in Examples 1-5 of the present invention on mouse tumor growth are as follows:

[0131] (1) Seventy male Kunming mice weighing 22 g were selected and used to 22 The cells were inoculated subcutaneously on the back of mice, with 0.1 mL per mouse and 2×10 6 The cells were randomly divided into 7 groups, with 10 in each group. After 8 days of feeding in an environment of 24°C, 50% humidity and 12 hours of light per day, the experimental groups 1-5 were gavaged with 0.165 g of the traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors prepared in Examples 1-5 every day, and the cyclophosphamide group was gavaged with 0.44 mg of cyclophosphamide every day, and 0.22 mL of 0.9% saline was used as the CK control group for 15 consecutive days.

[0132] (2) After 15 days, 70 mice were fasted but not watered for 8 h. Then, 15 mL of blood was collected from each mouse using a sterile tube under anesthesia. The blood was allowed to coagulate naturally at 28°C for 20 min and centrifuged at 2°C, 3000 r / min for 20 min. The mouse serum was collected and stored at -80°C for later use.

[0133] (3) After collecting blood from mice for 2 hours, the mice were killed by cervical dislocation, and 0.5 mL of normal saline was immediately injected into the back of the mice. The backs were rubbed for 2 minutes, the back skin was cut open, and the tumor tissues of the mice were quickly removed and stored in liquid nitrogen.

[0134] (4) Use an electronic balance to weigh the tumor weight of each mouse and calculate the average value of each group to obtain the mouse tumor weight (g). Use a vernier caliper to measure the longest diameter and shortest transverse diameter of the tumor, ensuring that the long and short diameters are in a vertical direction during measurement. Calculate the tumor volume according to the formula. Mouse tumor volume (mm 3 ) = (longest diameter × shortest transverse diameter 2 ) / 2;

[0135] (5) The mouse alanine aminotransferase kit (ELISA, catalog number: ml063179) and the mouse aspartate aminotransferase kit (ELISA, catalog number: ml058577) were used to measure the activities of alanine aminotransferase and aspartate aminotransferase in mouse serum. The obtained data were statistically analyzed using IBM SPSS Statistics 22.0 software, and graphics were drawn using Origin2022.

[0136] Table 1. Mouse tumor mass and volume measurement table

[0137]

[0138] Table 2. Enzyme activity determination in mouse serum

[0139]

[0140] Result analysis:

[0141] Figure 1 This is a graph showing the weight measurement results of the subcutaneous tumor on the back of mice described in Experimental Example 1 of the present invention. Figure 2 This is a graph showing the volume measurement results of the subcutaneous tumor on the back of mice described in Experimental Example 1 of the present invention. Figure 1 , Figure 2As shown in Table 1, after the traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors described in Examples 1-5 was taken by mice, the average weight of the subcutaneous tumor on the back of the mice was 0.37 g, 0.34 g, 0.28 g, 0.34 g, and 0.31 g, the average weight of the subcutaneous tumor on the back of the mice in the control group was 3.82 g, and the average weight of the subcutaneous tumor on the back of the mice in the cyclophosphamide treatment group was 2.45 g; after the traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors described in Examples 1-5 was taken by mice, the average volume of the subcutaneous tumor on the back of the mice was 2209.7 mm 3 , 2142.5 mm 3 、2068.5 mm 3 , 2162.2 mm 3 , 2097.7 mm 3 The average volume of subcutaneous tumors on the back of mice in the control group was 5186.5 mm 3 The average volume of subcutaneous tumor on the back of mice in the cyclophosphamide treatment group was 2845.3 mm 3 The weight and volume of the subcutaneous tumor on the back of the mice in Example 1-5 groups showed an extremely significant downward trend compared with the control group (P<0.001), indicating that the traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors described in the present invention can effectively inhibit the growth of subcutaneous tumors on the back of mice, and the tumor inhibition effect is significantly better than that of cyclophosphamide.

[0142] Figure 3 This is a graph showing the results of the determination of alanine aminotransferase and aspartate aminotransferase activities in mouse serum as described in Experimental Example 1 of the present invention. Figure 3As shown in Table 2, after the traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors described in Examples 1-5 was taken by mice, the average values ​​of alanine aminotransferase activity in the serum of mice were 3.7 U / L, 3.3 U / L, 2.6 U / L, 2.8 U / L, and 2.6 U / L, respectively, the average value of alanine aminotransferase activity in the serum of mice in the control group was 30.4 U / L, and the average value of alanine aminotransferase activity in the serum of mice in the cyclophosphamide treatment group was 18.0 U / L; after the traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors described in Examples 1-5 was taken by mice, the average values ​​of aspartate aminotransferase activity in the serum of mice were 27.4 U / L, 25.9 U / L, 24.0 U / L, 25.9 U / L, and 25.1 U / L, respectively, the average value of aspartate aminotransferase activity in the serum of mice in the control group was 64.4 U / L, and the average value of aspartate aminotransferase activity in the serum of mice in the cyclophosphamide treatment group was 40.6 U / L, alanine aminotransferase and aspartate aminotransferase in mouse serum are examination indicators of liver function. Increased activity indicates abnormal liver function and degeneration and necrosis of liver cells. The traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors described in the present invention can significantly (P<0.001) reduce the activity of alanine aminotransferase and aspartate aminotransferase, indicating that in the process of inhibiting tumor growth, there is little damage to liver function.

[0143] Experimental Example 2: Test to determine the immunosuppressive effects on liver cancer mice.

[0144] The experimental steps for determining the immunosuppressive effect of a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors prepared in Examples 1-5 of the present invention on mice with liver cancer are as follows:

[0145] (1) Seventy male Kunming mice weighing 22 g were selected and used to 22 The cells were inoculated into the peritoneal cavity of mice, with 0.1 mL per mouse and 2×10 6 The cells were randomly divided into 7 groups, with 10 in each group. After 8 days of feeding in an environment of 24°C, 50% humidity and 12 hours of light per day, the experimental groups 1-5 were gavaged with 0.165 g of the traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors prepared in Examples 1-5 every day, and the cyclophosphamide group was gavaged with 0.44 mg of cyclophosphamide every day, and 0.22 mL of 0.9% saline was used as the CK control group for 15 consecutive days.

[0146] (2) After 15 days, each Kunming mouse was intraperitoneally injected with 5% chicken red blood cells. Two hours later, the mice were killed by cervical dislocation, and 0.5 mL of normal saline was immediately injected into the peritoneal cavity. The abdominal cavity was rubbed for 2 min, the abdominal skin was cut open, 30 mL of peritoneal fluid was taken out and placed on a clean glass slide, covered with a coverslip, and incubated at 37°C for 30 min. After staining, the macrophages of the mice were observed and counted under an optical microscope, and the phagocytic rate and phagocytic index were calculated according to the formula: phagocytic rate (%) = number of macrophages phagocytosing chicken red blood cells / 2000×100%, and phagocytic index = total number of phagocytosed chicken red blood cells / number of macrophages phagocytosing chicken red blood cells. The obtained data were statistically analyzed using IBM SPSS Statistics 22.0 software, and graphics were drawn using Origin2022.

[0147] Table 3. Determination of phagocytic rate and phagocytic index of mouse macrophages

[0148]

[0149] Result analysis:

[0150] Figure 4 This is a graph showing the phagocytic rate of mouse macrophages described in Experimental Example 2 of the present invention. Figure 5 This is the result diagram of the mouse macrophage phagocytic index described in Experimental Example 2 of the present invention, as shown in Figure 4 , Figure 5 As shown in Table 3, after the traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors described in Examples 1-5 was taken by mice, the average phagocytic rate of chicken red blood cells by macrophages in the mice reached 75.3%, 76.8%, 79.6%, 76.8% and 78.2%, the average phagocytic rate of chicken red blood cells by macrophages in the control group mice was 36.3%, and the average phagocytic rate of chicken red blood cells by macrophages in the cyclophosphamide treatment group mice was 53.4%; after the traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors described in Example 3 was taken by mice, the average phagocytic index of chicken red blood cells by macrophages in the mice reached 0.93 at most, while the average phagocytic index of chicken red blood cells by macrophages in the control group mice was 0.49, and the average phagocytic index of chicken red blood cells by macrophages in the cyclophosphamide treatment group mice was 0.63, indicating that the traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors described in the present invention can also significantly (P<0.001) enhance the immune function of macrophages in mice in the process of inhibiting tumor growth.

[0151] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0152] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A method for preparing a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors, characterized in that: The specific steps include: Step 1: Put dried longan, Artemisia annua, Ashwagandha, Polygonum cuspidatum, Tripterygium wilfordii and Cordyceps sinensis into a powder grinder to obtain mixed Chinese herbal medicine powder; Step 2: put the ginseng peptide concentrate and the composite probiotic agent into a stirring tank, add the mixed Chinese herbal medicine powder prepared in step 1, and stir evenly to obtain a pre-fermentation mixture; Step 3, placing the pre-fermentation mixture prepared in step 2 into a fermentation tank for fermentation to obtain a Chinese medicine fermented longan ginseng peptide probiotic mixture; Step 4: putting the Chinese medicine fermented longan ginseng peptide probiotic mixture prepared in step 3 into a vacuum freeze dryer for vacuum freeze drying to obtain the Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors; The traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors is composed of the following raw materials in parts by weight: 180-230 parts of ginseng peptide concentrate, 100-150 parts of dried longan, 80-110 parts of artemisia annua, 60-90 parts of ashwagandha, 60-85 parts of knotweed, 50-70 parts of tripterygium wilfordii, 20-30 parts of cordyceps sinensis, and 45-70 parts of compound probiotic agent; The tumor is a liver cancer tumor; The composite probiotic agent is composed of bifidobacterium, Clostridium butyricum, Akkermansia muciniphila, and Lactobacillus reuteri, and the weight ratio is 1-4:2-3:1-2:1; The preparation method of the ginseng peptide concentrated solution specifically comprises the following steps: S1, weighing 100-150 parts by weight of ginseng roots and 30-40 parts by weight of American ginseng roots, mixing and crushing them, and then grinding them into powder to obtain mixed ginseng powder; S2, filtering the mixed ginseng powder prepared in S1 through a 200-300 mesh filter sieve to obtain fine ginseng powder; S3, stirring and dissolving the fine ginseng powder prepared in S2 with 300 parts by weight of ultrapure water to obtain a ginseng powder solution; S4, putting the ginseng powder solution prepared in S3 into an enzymolysis tank, adjusting the pH to 5.0 with 5 mol / L hydrochloric acid and heating to 50-60° C., adding 60-100 parts by weight of papain and 50-80 parts by weight of glucan exoenase, stirring and enzymolysis to obtain a ginseng peptide mixed solution; S5, centrifuging the ginseng peptide mixed solution prepared in S4, taking the supernatant to obtain a ginseng peptide solution; S6. Put the ginseng peptide solution prepared in S5 into a vacuum concentrator for vacuum concentration to obtain a ginseng peptide concentrated solution.

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