A stabilizing and repairing composition and its preparation method, application, and daily chemical product

Abstract

The present application belongs to the technical field of cosmetic production, and discloses a stabilizing and repairing composition, comprising 0.1-15% by mass of Centella asiatica extract, 1-3% by mass of 1,2-hexanediol, 10-80% by mass of 1,3-butanediol, 0.1-15% by mass of Gynostemma pentaphyllum extract, ginseng root extract, Panax notoginseng extract and the remainder of pure water, wherein the Gynostemma pentaphyllum extract, Panax ginseng root extract and Panax notoginseng extract are prepared by composite extraction, and during the extraction process, the mass ratio of Gynostemma pentaphyllum, Panax ginseng and Panax notoginseng is 1-3:3-20:0.5-2. The present application obtains a stabilizing and repairing composition with good hyaluronidase inhibition ability through the above design. In addition, the present application also discloses a preparation method of the stabilizing and repairing composition, the use of the stabilizing and repairing composition and a daily chemical product.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical product production, and in particular to a stabilization and repairing composition, a preparation method thereof, an application thereof, and a daily chemical product. Background Art

[0002] Hyaluronic acid (HA) is a biopolymer naturally present in human tissues, which has important physiological functions such as moisturizing, lubricating, and protecting cells. In skin tissue, HA can maintain the elasticity and moisture content of the skin, making the skin look younger and healthier. However, the degradation and metabolism of HA is also an important physiological process, in which hyaluronidase plays a key role.

[0003] Hyaluronidase is an enzyme protein that can degrade hyaluronic acid. It exists in various cells and tissues in the human body. Hyaluronidase breaks down hyaluronic acid into smaller fragments by hydrolyzing the β-1,4-glycosidic bonds of hyaluronic acid, thereby reducing its concentration in tissues. Under normal physiological conditions, the activity of hyaluronidase can maintain the balance level of hyaluronic acid, thereby maintaining the normal function of cells and tissues. However, when the activity of hyaluronidase increases abnormally or the synthesis of hyaluronic acid decreases, it will lead to excessive degradation of hyaluronic acid in tissues, thus affecting the health and youthfulness of the skin.

[0004] Research and technological development on inhibiting hyaluronidase are of great significance for maintaining the balanced level of hyaluronic acid in skin tissue. On the one hand, by inhibiting the activity of hyaluronidase, the degradation rate of hyaluronic acid in skin tissue can be slowed down, and its action time in the skin can be prolonged, thereby maintaining the elasticity and moisture content of the skin. On the other hand, inhibiting hyaluronidase can also reduce the excessive degradation products of hyaluronic acid, reduce irritation and damage to the skin, and help maintain the healthy state of the skin.

[0005] At present, the research on inhibiting hyaluronidase mainly focuses on two aspects: one is to find effective hyaluronidase inhibitors, and the other is to develop technologies and methods that can regulate the activity of hyaluronidase. At the same time, some studies have also shown that some plant extracts and natural products also have a certain effect of inhibiting hyaluronidase.

[0006] In addition to the field of drug research and development, the field of biotechnology is also exploring new technologies and methods that can inhibit the activity of hyaluronidase. For example, gene editing technology can be used to regulate the expression level of hyaluronidase genes, thereby reducing their activity. In addition, some nanotechnology and carrier technologies can also be applied to the directional delivery and targeted inhibition of hyaluronidase. These new technologies and methods provide new ways and possibilities for inhibiting hyaluronidase, and are expected to play an important role in the future fields of skin care and medical cosmetology.

[0007] Chinese patent application 202110663694.7 discloses a plant extract composite antibacterial agent and its application, including the following raw materials and their mass fractions: 1-30% antibacterial agent, 0.5-50% synergist and 20-98.5% solvent; the antibacterial agent is one or a mixture of cinnamon bark extract and peony root bark extract. The plant extract composite antibacterial agent prepared by this scheme has good antibacterial, antipruritic and anti-allergic effects, good safety, no toxic side effects on the human body, and can be widely used as an antibacterial and anti-allergic active ingredient in various skin care cosmetics;

[0008] Further observation of paragraph 12 of the description of the scheme shows that the synergistic enhancer of the scheme can be selected from one or a mixture of multiple plant extracts, including Gynostemma pentaphyllum extract, Ginseng root extract, Centella asiatica extract and Panax notoginseng extract. However, on the one hand, the above-mentioned plant additives appear more as synergists rather than main functional ingredients. On the other hand, the scheme does not provide specific examples of using the above-mentioned Gynostemma pentaphyllum extract, Ginseng root extract, Centella asiatica extract and Panax notoginseng extract as synergistic ingredients in the examples. Therefore, we cannot know which functions the above-mentioned plant extracts can enhance as the main ingredients.

[0009] In summary, the research and technological development of inhibiting hyaluronidase is of great significance for maintaining skin health and youthfulness. By finding effective inhibitors and developing new technologies, it is expected to achieve precise regulation of hyaluronidase activity, thereby delaying the degradation of hyaluronic acid in skin tissue, maintaining its balanced level in the skin, and promoting skin health and beauty.

[0010] Chinese patent application 202110573788.5 discloses a firming and wrinkle-lightening eye cream, comprising phase A, phase B, phase C, phase D and deionized water;

[0011] Phase A includes the following substances: cyclomethicone, pentaerythritol tetra(ethylhexanoate), Botrytis cistanche extract, Phytolacca americana extract, glycosphingolipids, and plant extracts;

[0012] Phase B includes the following substances: Penicillium cordata extract, white willow bark extract, silymarin, conditioning agent;

[0013] Phase C includes the following: arginine / lysine peptides, hydrogenated coconut glycerides, vegetable oils, and moisturizers;

[0014] Phase D includes the following substances: dipotassium glycyrrhizinate, dipropylene glycol, tocopherol acetate, emulsifier;

[0015] The eye cream provided by this solution has excellent skin tightening and wrinkle-reducing effects, and is gentle and non-irritating;

[0016] Further observation of the scheme shows that the plant extracts in its phase A include ginseng root extract and Panax notoginseng root extract;

[0017] By observing the embodiments of this scheme, it can be seen that although Examples 1-4 demonstrate plant extracts, it does not specify what kind of plant extracts are used in each embodiment, nor does it specify the mass ratio between the multiple plant extracts. Therefore, we cannot determine the specific effects and functions of the ginseng root extract and Panax notoginseng root extract in this scheme, and this scheme does not disclose the use of Centella asiatica extract.

[0018] The problem to be solved by this scheme is: how to develop a stabilizing and repairing composition with the ability to inhibit hyaluronidase. Summary of the invention

[0019] One of the purposes of the present application is to provide a stabilizing and repairing composition having the ability to inhibit hyaluronidase, wherein the stabilizing and repairing composition further enhances the hyaluronidase inhibition ability of the composition through the synergistic effect between Gynostemma pentaphyllum extract, Ginseng root extract, Panax notoginseng extract, and Centella asiatica extract;

[0020] Another object of the present application is to provide a method for preparing the above-mentioned stabilization and repair composition;

[0021] In addition, the present application also discloses the application of the stabilizing and repairing composition and a daily chemical product.

[0022] To achieve the above-mentioned purpose, the present application discloses a stabilization and repair composition, which comprises the following components by mass fraction:

[0023] Centella asiatica extract 0.1-15%;

[0024] The sum of the mass fractions of Gynostemma pentaphyllum extract, Ginseng root extract and Panax notoginseng extract is 0.1 to 15%;

[0025]

[0026] The gynostemma pentaphyllum extract, ginseng root extract and panax notoginseng extract are prepared by composite extraction, and during the extraction process, the mass ratio of gynostemma pentaphyllum, ginseng root and panax notoginseng is 1-3:3-20:0.5-2.

[0027] It should be noted that in the above formula, Centella asiatica is rich in a variety of active ingredients, such as polyphenols, vitamins and minerals, which can help protect the skin from free radical damage, reduce inflammatory responses, and moisturize and soothe the skin. Centella asiatica extract can also help improve skin elasticity and reduce the appearance of wrinkles and fine lines;

[0028] Gynostemma pentaphyllum extract is rich in flavonoids and its unique gypenosides, which have antioxidant effects and help protect the skin from free radical damage. At the same time, Gynostemma pentaphyllum also has anti-inflammatory properties, which can reduce skin inflammation and sensitive reactions, and help soothe redness, swelling and discomfort;

[0029] Panax notoginseng extract is rich in active ingredients such as notoginseng saponins, which can promote wound healing and repair damaged skin. It can help repair skin damage, reduce inflammatory reactions, promote skin cell regeneration, and accelerate the recovery of skin to a healthy state. It is also rich in antioxidant ingredients such as flavonoids, which can help resist the invasion of free radicals and slow down the aging process of the skin.

[0030] Ginseng root extract is rich in active ingredients such as ginsenosides, which can promote collagen synthesis and enhance skin elasticity. It helps reduce the appearance of wrinkles and fine lines, improve skin firmness, and delay the aging process of the skin. It is also rich in a variety of vitamins, minerals, and amino acids, which can provide sufficient nutrition for the skin, enhance the skin's moisturizing ability, and improve skin dryness and roughness.

[0031] Preferably, the following components are included by mass fraction:

[0032]

[0033]

[0034] The gynostemma pentaphyllum extract, ginseng root extract and panax notoginseng extract are prepared by composite extraction, and during the extraction process, the mass ratio of gynostemma pentaphyllum, ginseng root and panax notoginseng is 1.6-2.5:5-15:0.8-1.2.

[0035] Preferably, the extraction method of the Centella asiatica extract is to dry and crush the Centella asiatica, then use 20% by mass ethanol as an extraction solvent, soak at room temperature for 6 to 8 hours, then centrifuge and take the supernatant to obtain the Centella asiatica extract.

[0036] Preferably, the specific method of the composite extraction is:

[0037] Step 1: crushing Gynostemma pentaphyllum, ginseng root and Panax notoginseng, passing through a 60-mesh sieve to obtain Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder respectively, and then weighing Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder according to the mass ratio of Gynostemma pentaphyllum, ginseng root and Panax notoginseng of 1-3:3-20:0.5-2 to obtain powder;

[0038] Step 2: mixing the lyophilic salt and water to prepare a mixed solution;

[0039] Step 3: mixing an ethanol solution having a concentration of 20 to 30% with the mixed solution obtained in step 2 to obtain a two-phase aqueous solvent;

[0040] Step 4: Add the powder obtained in step 1 into the aqueous two-phase solvent obtained in step 3, shake well, perform ultrasonic extraction, take the supernatant, and freeze-dry to obtain a composite extract consisting of Gynostemma pentaphyllum extract, Ginseng root extract, and Panax notoginseng extract.

[0041] In addition, the present application also discloses a method for preparing a stabilizing and repairing composition, which comprises mixing and dispersing Centella asiatica extract, Gynostemma pentaphyllum extract, Panax ginseng root extract, Panax notoginseng extract, 1,2-hexanediol, 1,3-butanediol, and pure water to obtain a stabilizing and repairing composition.

[0042] In addition, the present application also discloses the use of the stabilization and repair composition as described above as an active ingredient in daily chemical products.

[0043] In addition, the present application also discloses a daily chemical product containing 0.1 to 50% by mass of the stabilizing and repairing composition described above.

[0044] Preferably, the daily chemical product is a patch, an ointment, a liquid, a spray, a gel, an emulsion, or a cream.

[0045] Preferably, the daily chemical product is gel, essence, toner, facial mask, cleanser, lotion, perfume, makeup remover, lipstick, blush, or spray.

[0046] The beneficial effects of this application are:

[0047] The Centella asiatica extract in the stabilizing and repairing composition prepared in the present application is rich in triterpenoid compounds, such as asiaticoside and asiatic acid, which can stimulate the activity of fibroblasts and increase the synthesis of collagen. This helps the elasticity and firmness of the skin, thereby reducing fine lines and wrinkles;

[0048] Gynostemma pentaphyllum extract is rich in flavonoids and its unique gypenosides, which have antioxidant effects and help protect the skin from free radical damage. At the same time, Gynostemma pentaphyllum also has anti-inflammatory properties, which can reduce skin inflammation and sensitive reactions, and help soothe redness, swelling and discomfort;

[0049] Panax notoginseng extract is rich in active ingredients such as notoginseng saponins, which can promote wound healing and repair damaged skin. It can help repair skin damage, reduce inflammatory reactions, promote skin cell regeneration, and accelerate the recovery of skin to a healthy state. It is also rich in antioxidant ingredients such as flavonoids, which can help resist the invasion of free radicals and slow down the aging process of the skin.

[0050] Ginseng root extract is rich in active ingredients such as ginsenosides, which can promote collagen synthesis and enhance skin elasticity. It helps reduce the appearance of wrinkles and fine lines, improve skin firmness, and delay the aging process of the skin. It is also rich in a variety of vitamins, minerals, and amino acids, which can provide sufficient nutrition for the skin, enhance the skin's moisturizing ability, and improve skin dryness and roughness.

[0051] At the same time, the above four plant extracts produce a synergistic effect on the inhibitory ability of hyaluronidase, significantly improving the inhibitory ability of the composition on hyaluronidase. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 This is a schematic diagram of a chicken embryo before the solution prepared by the composition of Example 1 is added dropwise;

[0053] Figure 2 This is a schematic diagram of a chicken embryo after the solution prepared by the composition of Example 1 is added dropwise;

[0054] Figure 3 This is a photo of the cell healing status of the sample group at 0h;

[0055] Figure 4 This is a photo of the cell healing status of the sample group at 24 hours. DETAILED DESCRIPTION

[0056] The present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. In the description of the present invention, it should be noted that, if no specific conditions are specified in the embodiments, the conditions are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0057] Before presenting the examples, the extraction process of the plant extracts used in the examples and the raw materials involved in each example are first explained as follows:

[0058] The extraction method of the Centella asiatica extract is to dry the Centella asiatica, crush it, and pass it through a 40-mesh sieve, then use 20% ethanol by mass as an extraction solvent, soak it at room temperature for 6 hours, then centrifuge it, take the supernatant, and obtain the Centella asiatica extract;

[0059] The gynostemma pentaphyllum extract, ginseng root extract and Panax notoginseng extract are obtained by a composite extraction method, which specifically includes the following steps:

[0060] Step 1: crushing Gynostemma pentaphyllum, ginseng root and Panax notoginseng, passing through a 60-mesh sieve to obtain Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder respectively, and then weighing Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder according to the mass ratio of Gynostemma pentaphyllum, ginseng root and Panax notoginseng of 2:8:1 to mix and obtain powder;

[0061] Step 2: mixing sodium carbonate and water to obtain a mixed solution having a mass fraction of 30% sodium carbonate;

[0062] Step 3: Mixing the mixed solution with an ethanol solution having a mass fraction of 20% at a mass ratio of 1:1 to obtain a two-phase aqueous solvent;

[0063] Step 4: Add the powder obtained in step 1 into a two-phase aqueous solvent at a solid-liquid ratio of 1:15, shake well, perform ultrasonic extraction, take the supernatant, remove sodium carbonate, and freeze-dry to obtain a composite extract consisting of a Gynostemma pentaphyllum extract, a ginseng root extract, and a Panax notoginseng extract.

[0064] At the same time, it should be noted that in the embodiments and comparative examples, the preparation method of the stabilization and repair composition is:

[0065] The Centella asiatica extract, the composite extract, 1,2-hexanediol, 1,3-butanediol and pure water are mixed and dispersed to obtain a stabilizing and repairing composition.

[0066] The raw materials involved in the embodiment are shown in Table 1:

[0067] Table 1

[0068]

[0069] Examples 1-4

[0070] A stabilization and repair composition, the formula of which is shown in Table 2:

[0071] Table 2

[0072]

[0073] Example 5

[0074] It is basically the same as Example 1, except that during the extraction process, the total mass of Gynostemma pentaphyllum, ginseng root and Panax notoginseng remains unchanged, but the mass ratio of Gynostemma pentaphyllum, ginseng and Panax notoginseng is 1.6:15:0.8.

[0075] Example 6

[0076] It is basically the same as Example 1, except that during the extraction process, the total mass of Gynostemma pentaphyllum, ginseng root and Panax notoginseng remains unchanged, but the mass ratio of Gynostemma pentaphyllum, ginseng and Panax notoginseng is 2.5:5:1.2.

[0077] Example 7

[0078] The method is basically the same as Example 1, except that, in the composite extraction method of the composite extract, K2HPO4 and water are mixed to prepare a mixed solution.

[0079] Example 8

[0080] The method is basically the same as Example 1, except that, in the composite extraction method of the composite extract, KBrO3 salt and water are mixed to prepare a mixed solution.

[0081] Comparative Example 1

[0082] The method is basically the same as Example 1, except that, in the extraction process of the Gynostemma pentaphyllum extract, the Ginseng root extract, and the Panax notoginseng extract, step 1 is:

[0083] Step 1: crush Gynostemma pentaphyllum, ginseng root and Panax notoginseng, pass through a 60-mesh sieve to obtain Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder respectively, then weigh Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder according to the mass ratio of Gynostemma pentaphyllum, ginseng root and Panax notoginseng of 1:25:0.5 and mix to obtain powder;

[0084] It should be noted that in step 1, the total mass of Gynostemma pentaphyllum, ginseng root and Panax notoginseng is the same as that in Example 1.

[0085] Comparative Example 2

[0086] The method is basically the same as Example 1, except that, in the extraction process of the Gynostemma pentaphyllum extract, the Ginseng root extract, and the Panax notoginseng extract, step 1 is:

[0087] Step 1: crush Gynostemma pentaphyllum, ginseng root and Panax notoginseng, pass through a 60-mesh sieve to obtain Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder respectively, then weigh Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder according to the mass ratio of Gynostemma pentaphyllum, ginseng root and Panax notoginseng of 5:3:0.5 and mix to obtain powder;

[0088] It should be noted that in step 1, the total mass of Gynostemma pentaphyllum, ginseng root and Panax notoginseng is the same as that in Example 1.

[0089] Comparative Example 3

[0090] The method is basically the same as Example 1, except that, in the extraction process of the Gynostemma pentaphyllum extract, the Ginseng root extract, and the Panax notoginseng extract, step 1 is:

[0091] Step 1: crushing Gynostemma pentaphyllum, ginseng root and Panax notoginseng, passing through a 60-mesh sieve to obtain Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder respectively, and then weighing Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder according to the mass ratio of Gynostemma pentaphyllum, ginseng root and Panax notoginseng of 1:3:3 to obtain powder;

[0092] It should be noted that in step 1, the total mass of Gynostemma pentaphyllum, ginseng root and Panax notoginseng is the same as that in Example 1.

[0093] Comparative Examples 4-7

[0094] A composition, the formula is shown in Table 3:

[0095] Table 3

[0096]

[0097]

[0098] It should be noted that in Comparative Examples 4-7, the total addition amount of the composite extract is 5%. Specifically, in Comparative Example 4, when the total addition amount of the composite extract is 5%, the mass ratio of Gynostemma pentaphyllum, Ginseng root, and Panax notoginseng during the extraction of the composite extract is 2:8:1;

[0099] Specifically, in Comparative Example 5, when the total addition amount of the composite extract was 5%, during the extraction of the composite extract, the mass ratio of Gynostemma pentaphyllum, Ginseng root, and Panax notoginseng was 0:8:1;

[0100] Specifically, in Comparative Example 6, when the total addition amount of the composite extract was 5%, during the extraction of the composite extract, the mass ratio of Gynostemma pentaphyllum, Ginseng root, and Panax notoginseng was 2:0:1;

[0101] Specifically, in Comparative Example 7, when the total addition amount of the composite extract was 5%, during the extraction of the composite extract, the mass ratio of Gynostemma pentaphyllum, Ginseng root and Panax notoginseng was 2:8:0.

[0102] Comparative Examples 8-9

[0103] A composition, the formula is shown in Table 4:

[0104] Table 4

[0105]

[0106]

[0107] Comparative Examples 10-12

[0108] A composition, the formula is shown in Table 5:

[0109] Table 5

[0110]

[0111] The composite extract in Comparative Example 10 is prepared by composite extraction of ginseng root and Panax notoginseng, and during the extraction process, the mass ratio of ginseng to Panax notoginseng is 8:1;

[0112] The composite extract in Comparative Example 11 is prepared by composite extraction of Gynostemma pentaphyllum and Panax notoginseng, and during the extraction process, the mass ratio of Gynostemma pentaphyllum to Panax notoginseng is 2:1;

[0113] The composite extract in Comparative Example 12 is prepared by composite extraction of Gynostemma pentaphyllum and ginseng root, and during the extraction process, the mass ratio of Gynostemma pentaphyllum to ginseng is 2:8.

[0114] Comparative Example 13

[0115] The method is basically the same as Example 1, except that honeysuckle is used instead of Gynostemma pentaphyllum in the extraction process of the composite extract.

[0116] Comparative Example 14

[0117] The method is basically the same as Example 1, except that, in the extraction process of the composite extract, Sophora flavescens root is used instead of ginseng root.

[0118] Comparative Example 15

[0119] The method is basically the same as Example 1, except that Angelica sinensis is used instead of Panax notoginseng in the extraction process of the composite extract.

[0120] Comparative Example 16

[0121] A composition, the formula is shown in Table 6:

[0122] Table 6

[0123]

[0124] Performance Testing

[0125] 1.1 Irritation test

[0126] According to the standard SN / T2329-2009 "Chicken Embryo Chorioallantoic Membrane Test for Cosmetic Irritation": For a 9-day-old egg, peel off the eggshell from the air chamber end with tweezers, soak the inner membrane with 0.9% saline, and then carefully peel off the egg membrane from the edge to reveal the chorioallantoic membrane.

[0127] The compositions prepared in Examples 1 to 8 and Comparative Examples 1 to 16 were diluted with deionized water to obtain a solution with a mass fraction of 5%, and then 0.3 mL of the solution was dripped onto the membrane using a pipette, and the changes in the blood vessels of the chorioallantoic membrane were observed after 3 minutes of timing. Each sample was tested 6 times in parallel.

[0128] Chicken embryo score = the average of single chicken embryo scores in 6 experiments, where the single chicken embryo score is the sum of the bleeding, coagulation and vascular melting observed for each chicken embryo, and the result is rounded to two decimal places.

[0129] The scoring criteria for bleeding, coagulation and vascular lysis are shown in Table 7:

[0130] Table 7

[0131]

[0132]

[0133] Rating criteria: ES≤12: no / mild irritation; 12<ES<16: moderate irritation; ES≥16: strong irritation.

[0134] According to the test, the embodiments and comparative examples did not produce irritation. Figure 1-2 , which is a comparison of the states of chicken embryos before and after the solution prepared from the composition of Example 1 is added dropwise to chicken embryos. According to statistics, the ES value of the solution prepared from the composition of Example 1 is 3.67.

[0135] 1.2 Hyaluronidase inhibition rate test

[0136] Control and test sample treatment

[0137] Samples: the stabilization and repair compositions of Examples 1 to 8 and the compositions of Comparative Examples 1 to 16.

[0138] Negative control: a mixture of 1,2-hexanediol, 1,3-butanediol and pure water, wherein the mass fraction of 1,2-hexanediol is 2.2% and the mass fraction of 1,3-butanediol is 70%.

[0139] Test operation steps

[0140] Set up a sample group, a sample background group, a solvent group and a solvent background group. Each group needs to have 3 parallels. Add different reagent solutions to the four groups respectively, shake well, and leave at room temperature for 30 minutes for color development. Use a UV spectrophotometer to measure the absorbance value at a wavelength of 528nm.

[0141] Calculation formula:

[0142]

[0143] Where: A—is the absorbance of the reaction solution without sample;

[0144] B—is the absorbance of the reaction solution without sample and enzyme;

[0145] C—is the absorbance of the reaction solution containing sample and enzyme;

[0146] D—is the absorbance of the reaction solution containing sample and without enzyme.

[0147] Data analysis

[0148] The statistical analysis software was SPSS, and the comparison of hyaluronidase inhibition rates between the test samples, positive controls, and negative controls was performed using an independent sample t-test. The above statistical analyses were all two-tailed tests, with a significance level of a = 0.05. P ≥ 0.05 indicated that there was no significant difference between the two groups; P < 0.05 indicated that there was a significant difference between the two groups.

[0149] The test results are shown in Table 8:

[0150] Table 8

[0151]

[0152] Result analysis:

[0153] 1. It can be seen from Examples 1-4 that when the addition amounts of Centella asiatica extract, composite extract, 1,2-hexanediol and 1,3-butanediol are slightly changed, there is a certain difference in the inhibition rate of hyaluronidase in the stabilizing and repairing compositions prepared in Examples 1-4, but the difference is small. At the same time, the stabilizing and repairing compositions prepared in Example 1 have a higher hyaluronidase inhibition rate than those in Examples 2-4. Therefore, the formula of Example 1 is the best formula among Examples 1-4;

[0154] 2. It can be seen from Examples 1 and 5-6 that when the total mass of Gynostemma pentaphyllum, ginseng root extract and Panax notoginseng extract is unchanged during the composite extraction process, when the mass ratio of Gynostemma pentaphyllum, ginseng and Panax notoginseng is 2:8:1, the stabilization and repair composition has a more excellent hyaluronidase inhibition rate. We believe that the reason for this phenomenon may be that when the Gynostemma pentaphyllum extract, ginseng root extract and Panax notoginseng extract are compositely extracted, when the mass ratio of Gynostemma pentaphyllum, ginseng and Panax notoginseng is 2:8:1, the polysaccharides produced by the three during the extraction process increase the solubility of saponins such as ginsenosides and Gynostemma pentaphyllum saponins in the aqueous two-phase system, thereby increasing the extraction amount of saponins, thereby increasing the inhibitory ability of the composite extract on hyaluronidase;

[0155] 3. It can be seen from Examples 1 and 7-8 that when other lyophilic salts are used to replace sodium carbonate, the inhibitory ability of the compositions prepared in Examples 7 and 8 on hyaluronidase shows a certain downward trend. We believe that in the process of composite extraction of Gynostemma pentaphyllum extract, Ginseng root extract and Panax notoginseng extract, the two-phase aqueous system prepared with sodium carbonate as the lyophilic salt is more suitable for the extraction of the composite extract, so that the composite extract has better hyaluronidase inhibitory ability;

[0156] 4. It can be seen from Example 1 and Comparative Examples 1-3 that in the extraction process of the composite extract, when the content of any one of Gynostemma pentaphyllum, Ginseng, and Panax notoginseng is too high, the inhibitory ability of the composition to hyaluronidase decreases to varying degrees. We speculate that the reason for this phenomenon may be that when the content of any one of Gynostemma pentaphyllum, Ginseng, and Panax notoginseng is too high, the synergistic effect between the three is broken, which leads to a decrease in the hyaluronidase inhibitory ability of the composition;

[0157] 5. It can be seen from Example 1 and Comparative Examples 5-7 that when the composite extract lacks any one of Gynostemma pentaphyllum, Ginseng, and Panax notoginseng during the extraction process, the hyaluronic acid inhibition ability of the composition shows a significant downward trend. At the same time, in Comparative Examples 4-7 relative to Comparative Example 16, the hyaluronic acid inhibition rate of Comparative Example 16 is 40.581% when the composite extract is completely absent. However, when Comparative Examples 5-7 contain composite extracts obtained by simultaneously containing any two of Gynostemma pentaphyllum, Ginseng, and Panax notoginseng, the hyaluronidase inhibition rates of Comparative Examples 5-7 are 42.01 and 43.02%, respectively. 6%, 41.898%, 41.101%, which are about 1.5%, 1.3%, and 0.5% higher than those of Comparative Example 16, respectively; in this case, if the inhibitory ability of the composite extract on the hyaluronidase of the composition is only produced by the simple superposition of Gynostemma pentaphyllum, Ginseng, and Panax notoginseng, then the theoretical hyaluronidase inhibition rate of Example 1 is about 42.28%, but the actual hyaluronidase inhibition rate of Example 1 is 49.711%. It can be seen that a synergistic effect is produced between Gynostemma pentaphyllum, Ginseng, and Panax notoginseng, thereby enhancing the hyaluronic acid inhibition ability of the composition;

[0158] 6. It can be seen from Example 1 and Comparative Examples 8-9 that after the Centella asiatica extract is replaced with the asiatica glycoside rich in Centella asiatica or the extract of Bupleurum chinense which is biologically close to Centella asiatica, the hyaluronidase inhibition ability of the composition shows a significant downward trend. It can be seen that, on the one hand, it is not only the asiatica glycoside rich in Centella asiatica that promotes the inhibition ability of the composition in this scheme, and on the other hand, the Centella asiatica extract is irreplaceable in this scheme;

[0159] 7. It can be seen from Example 1 and Comparative Examples 10-12 that when Gynostemma pentaphyllum, Ginseng, and Panax notoginseng are missing in the preparation process of the composite extract, and Gynostemma pentaphyllum saponin, Ginseng saponin, and Panax notoginseng saponin are used to make up for the missing composite extract, the hyaluronidase inhibition rate of Comparative Examples 10-12 shows a significant decline. It can be seen that when the corresponding plant extracts are replaced with saponin components rich in Gynostemma pentaphyllum, Ginseng, and Panax notoginseng, the hyaluronidase inhibition ability of the composition will decrease to a certain extent. Therefore, we believe that the reason for the increase in hyaluronidase in the composition is not only the saponin component in the composite extract, but also other components such as polysaccharides and flavonoids may also have the effect of improving the hyaluronidase inhibition ability of the composition;

[0160] 8. It can be seen from Example 1 and Comparative Examples 13-15 that in the preparation process of the composite extract, after using honeysuckle, sophora flavescens, and angelica to replace Gynostemma pentaphyllum, ginseng, and Panax notoginseng in sequence, the hyaluronidase inhibition ability of the compositions prepared in Comparative Examples 13-15 all showed a significant downward trend. It can be seen that in the present application, any one of Gynostemma pentaphyllum, ginseng, and Panax notoginseng is irreplaceable;

[0161] 9. It can be seen from Example 1 and Comparative Examples 4 and 16 that when the composition lacks any component of the Centella asiatica extract or the composite extract, the hyaluronidase inhibition abilities of Comparative Examples 4 and 16 are only 40.697% and 40.581%, respectively, which are about 3.04% and 2.9% higher than the negative control, respectively. If Example 1 is obtained by simply superimposing the Centella asiatica extract and the composite extract, then the theoretical hyaluronidase inhibition rate of Example 1 is about 43.65%, but the actual hyaluronidase inhibition rate of Example 1 reaches 49.711%. It can be seen that Example 1 has such a high hyaluronidase inhibition rate obtained after the synergistic effect of the Centella asiatica extract and the composite extract.

[0162] 1.3 Cell scratch test

[0163] 1.3.1 Experimental materials and methods

[0164] MF52-N inverted fluorescence microscope, mouse fibroblasts: 3T3 cells

[0165] 1.3.2 Test methods

[0166] (1) Treatment of test samples

[0167] Sample group: The stabilizing and repairing composition prepared in Example 1 was diluted with pure water to a solution with a mass fraction of 0.5%, and then filtered and collected with a 0.22 μm filter to obtain a sample group.

[0168] Negative control: basic culture medium (cell activity test); incomplete culture medium (scratch test).

[0169] Positive control: basal culture medium.

[0170] (2) Test operation steps

[0171] Cell activity test: 3T3 cells were plated in 96-well plates. After 24 hours, the culture medium was discarded and basal culture medium containing different concentrations of test samples was added. After 24 hours, the OD490nm was detected by MTT method, and the effect of the test samples on the activity of 3T3 cells was analyzed by t-test.

[0172] Fibroblast scratch test: After fibroblasts were plated on a 6-well plate and cultured for 24 hours, a line was drawn in the middle of the 6-well plate with a sterile pipette tip to produce a scratch, and the culture medium was discarded. The basal culture medium (positive control), incomplete culture medium (negative control), and culture medium of the corresponding sample group were added respectively, and the cell healing was observed at 0h and 24h, and photos were taken;

[0173] (3) The calculation formula is shown in Formula 2:

[0174]

[0175] (4) Data analysis

[0176] Image-pro plus (IPP) image analysis software was used to quantitatively analyze the cell scratch images at 0h and 24h, and the 24h scratch healing rate of the sample group was 47.31%. Figure 3-4 shown.

[0177] Application Examples

[0178] A stabilizing and repairing composition spray water consists of phase A, phase B, phase C and phase D.

[0179] Phase A contains the following components in percentage by weight: 74.39% water.

[0180] Phase B contains the following components in percentage by weight: 3% 1,3-butanediol, 0.5% p-hydroxyacetophenone.

[0181] Phase C contains the following components in percentage by weight: 0.5% niacinamide, 0.01% sodium hyaluronate.

[0182] Phase D comprises the following components in percentage by mass: 1% hydroxyethyl urea, 0.6% 1,2-hexanediol, and 20% stabilizing and repairing composition.

[0183] A stabilization and repair composition spray water is prepared. Phase A is added into an emulsifying pot and heated to 85°C; Phase B is mixed, heated to 85°C, dissolved until completely transparent, and then added into the emulsifying pot; homogenization is performed at 2500 r / min, and Phase C is added in sequence. After homogenization for 3 minutes, the mixture is stopped, and the mixture is stirred at 200 r / min for 30 minutes while keeping the mixture warm; the mixture is then cooled to 45°C, Phase D is added, and the mixture is stirred evenly to obtain a stabilization and repair composition spray water.

[0184] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. A stabilization and repair composition, characterized in that: By mass fraction, it includes the following components: Centella asiatica extract 0.1-15%; The sum of the mass fractions of Gynostemma pentaphyllum extract, Ginseng root extract, and Panax notoginseng extract is 0.1-15%; 1,2-Hexanediol 1-3%; 1,3-Butanediol 10-80%; Pure water was added to 100%; The gynostemma pentaphyllum extract, ginseng root extract and panax notoginseng extract are prepared by composite extraction, and during the extraction process, the mass ratio of gynostemma pentaphyllum, ginseng root and panax notoginseng is 1-3:3-20:0.5-2; The extraction method of the Centella asiatica extract is to dry and crush the Centella asiatica, then use 20% by mass ethanol as an extraction solvent, soak it at room temperature for 6 to 8 hours, then centrifuge and take the supernatant to obtain the Centella asiatica extract; The Gynostemma pentaphyllum extract, the Ginseng root extract and the Panax notoginseng extract are prepared by composite extraction, and the specific method of the composite extraction is as follows: Step 1: crushing Gynostemma pentaphyllum, ginseng root and Panax notoginseng, passing through a 60-mesh sieve to obtain Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder respectively, and then weighing Gynostemma pentaphyllum powder, ginseng root powder and Panax notoginseng powder according to the mass ratio of Gynostemma pentaphyllum, ginseng root and Panax notoginseng of 1-3:3-20:0.5-2 to obtain powder; Step 2: mixing the lyophilic salt and water to prepare a mixed solution; Step 3: mixing an ethanol solution having a concentration of 20 to 30% with the mixed solution obtained in step 2 to obtain a two-phase aqueous solvent; Step 4: Add the powder obtained in step 1 into the aqueous two-phase solvent obtained in step 3, shake well, perform ultrasonic extraction, take the supernatant, and freeze-dry to obtain a composite extract consisting of Gynostemma pentaphyllum extract, Ginseng root extract, and Panax notoginseng extract.

2. The stabilization and repair composition according to claim 1, characterized in that: By mass fraction, it includes the following components: Centella asiatica extract 0.5-10%; The sum of the mass fractions of Gynostemma pentaphyllum extract, Ginseng root extract, and Panax notoginseng extract is 0.2-10%; 1,2-Hexanediol 1.5-2.5%; 1,3-Butanediol 40-75%; Pure water was added to 100%; The gynostemma pentaphyllum extract, ginseng root extract and panax notoginseng extract are prepared by composite extraction, and during the extraction process, the mass ratio of gynostemma pentaphyllum, ginseng root and panax notoginseng is 1.6-2.5:5-15:0.8-1.

2.

3. A method for preparing the stabilization and repair composition according to any one of claims 1 to 2, characterized in that: Centella asiatica extract, Gynostemma pentaphyllum extract, ginseng root extract, Panax notoginseng extract, 1,2-hexanediol, 1,3-butanediol and pure water are mixed and dispersed to obtain a stabilizing and repairing composition.

4. Use of the stabilization and repair composition as described in any one of claims 1 to 2 in the preparation of daily chemical products.

5. A daily chemical product, characterized in that: Contains 0.1 to 50% by mass of the stabilization and repair composition as described in any one of claims 1 to 2.

6. The daily chemical product according to claim 5, characterized in that: The daily chemical products are patches, ointments, liquids, sprays, gels, emulsions, and creams.

7. The daily chemical product according to claim 5, characterized in that: The daily chemical products are gel, essence, toner, facial mask, cleanser, lotion, perfume, make-up remover, lipstick, blush and spray.

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