A method for preparing textiles with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties

Abstract

The present invention discloses a method for preparing textiles with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties, belonging to the field of functional finishing of textiles. The present invention provides a method for modifying glucose oxidase using a preferred protein as a modifier and EDC / NHS as a cross-linking agent. The modified glucose oxidase can quickly and gently aggregate on the surface of the textile under the action of a reducing agent to form a uniform coating. The coating can not only effectively inhibit bacteria for a long time, but also can quickly catalyze the glucose in the sweat to generate hydrogen peroxide for rapid sterilization after contacting sweat. In addition, the coating can significantly improve the hydrophilicity of the fabric and can withstand multiple cycles of water washing. The present invention uses eco-friendly additives that are harmless to the environment and human body while ensuring antibacterial properties. This method is simple to operate, safe and environmentally friendly, and has high universality. It plays an important role in increasing the added value of textiles and their comprehensive utilization and development.

Description

Technical Field

[0001] The invention relates to the technical field of functional finishing of fabrics, in particular to a preparation method of textiles capable of improving perspiration efficiency and strengthening rapid antibacterial properties through sweating. Background Art

[0002] Under suitable temperature and humidity conditions, fabrics are prone to attracting fungi, bacteria, and microorganisms, creating conditions for their proliferation and spread. Bacterial growth can produce unpleasant odors and may lead to skin or respiratory diseases. Therefore, antimicrobial functionalization of textiles is widely demanded in everyday wear, military, and healthcare markets. While current antimicrobial finishing of fabrics meets consumer needs, sweating after exercise or physical activity significantly impacts the wearer's physical feel. Furthermore, sweat immersion leads to rapid bacterial growth. Therefore, fabrics with improved perspiration wicking efficiency and rapid inhibition of bacterial growth are considered beneficial for maintaining comfort and protecting human health. To enhance antimicrobial properties, a variety of antimicrobial agents, such as metal nanoparticles, metal oxides, quaternary ammonium salts, and halamines, are used to finish fabrics. However, these antimicrobial agents are often unfriendly to the environment and the human body. Natural antimicrobial agents, such as chitosan and aloe vera extracts from plants and animals, often have weak binding to the fabric substrate and poor antimicrobial performance. Fabrics that offer long-lasting antimicrobial protection during daily use and rapid sterilization when exposed to sweat are in high demand.

[0003] Sweat, as a metabolic product of the human body, is rich in small molecules such as Na + 、Cl - , K + , lactate, calcium, glucose, ammonia, ethanol, urea, cortisol, and various neuropeptides and cytokines. The concentration of glucose in human sweat ranges from 10 to 200 μmol / L. Glucose oxidase (GOx) catalyzes the oxidation of glucose into gluconic acid and hydrogen peroxide (H2O2). This principle is often used to design biosensors to detect human metabolic products. Hydrogen peroxide solution, also known as hydrogen peroxide, is a strong oxidant, with a common disinfection concentration of 2.5%-3.5%, which is effective against bacteria and viruses. Free enzymes are easily inactivated and difficult to recycle. Glucose oxidase is typically immobilized on fabric surfaces through adsorption, cross-linking, encapsulation, and covalent bonding. Adsorption methods generally do not provide long-term binding. Cross-linking agents, such as glutaraldehyde and genipin, are commonly used. However, cross-linking agents have an unpleasant odor and can impart undesirable color to the fabric surface. The encapsulated enzyme remains fixed to the fabric for a further step. Covalent bonding often requires modification of the fabric to make it reactive. Summary of the Invention

[0004] The technical problems to be solved by the present invention are: poor perspiration efficiency of fabrics in the presence of a large amount of sweat and easy breeding of bacteria; a simple and universal method for fixing glucose oxidase on the surface of fabrics; and the stability and ecological and environmental performance of antibacterial fabrics during long-term use.

[0005] To address the above technical problems, the present invention provides a method for preparing textiles with improved perspiration-wicking efficiency and sweat-enhanced rapid antibacterial properties. During wear, these textiles, thanks to their excellent hydrophilicity, evenly diffuse sweat across the fabric surface, preventing discomfort caused by dampness and coldness. This invention has potential applications in improving the antibacterial properties of textiles and in the development of sportswear fabrics.

[0006] The technical solutions of the present invention are as follows:

[0007] The first object of the present invention is to provide a method for preparing a textile having improved perspiration efficiency and sweat-enhanced rapid antibacterial properties, the method comprising the following steps:

[0008] (1) Glucose oxidase was dissolved in 2-(N-morpholino)ethanesulfonic acid (MES) buffer solution, and then a cross-linking agent 1-ethyl-3-(3-xylyl)iminomethyltetrafluoroborate (EDC) / N-hydroxysuccinimide (NHS) was added, and the mixture was stirred at 20-40°C for 20-60 minutes to prepare solution a; protein was dissolved in MES buffer solution to prepare solution b; an equal volume of solution b was added dropwise to solution a, and the mixture was stirred in the dark at 2-6°C for 8-16 hours. After the reaction was completed, the mixture was dialyzed with deionized water at 2-6°C for 24-60 hours, and the remaining solid product was freeze-dried for 36-72 hours to prepare the modified glucose oxidase;

[0009] (2) dissolving the modified glucose oxidase in Tris-HCl (tris(hydroxymethyl)aminomethane hydrochloride) buffer to prepare a 1-20 g / L solution; dissolving the reducing agent in Tris-HCl buffer to prepare a 5-100 mmol / L solution;

[0010] (3) Mixing the two solutions in step (2) in equal volumes, placing the textile flat in the mixed solution, reacting at 30-40° C. for 2-8 hours, taking out the fabric and drying it, thereby obtaining the textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties.

[0011] In one embodiment of the present invention, in step (1), the pH of the MES buffer solution is 4.5-6; the concentration of glucose oxidase in the solution a is 5-50 μmol / L.

[0012] In one embodiment of the present invention, in step (1), the cross-linking agent is EDC / NHS, the molar ratio of EDS to NHS is 1:1; and the molar ratio of glucose oxidase to the cross-linking agent is 1:2.

[0013] EDC (1-ethyl-3-(3-xylyl)iminomethyl tetrafluoroborate), NHS (N-hydroxysuccinimide).

[0014] In one embodiment of the present invention, in step (1), the protein is one or more of lysozyme, bovine serum albumin, α-lactalbumin, insulin, and β-lactoglobulin; and the concentration of the protein in solution b is 50-150 μmol / L.

[0015] In one embodiment of the present invention, in step (1), the molecular weight cut-off of the dialysis membrane used for dialysis is 14-50 kDa; the freeze-drying conditions are: temperature -20-40°C, vacuum degree 10-20 Pa.

[0016] In one embodiment of the present invention, in step (2), the pH of the Tris-HCl buffer is 6.5-7.5.

[0017] In one embodiment of the present invention, in step (2), the reducing agent is one or more of thiourea dioxide, L-cysteine, tris(2-carboxyethyl)phosphine hydrochloride, mercaptoethanol, reduced glutathione, and vitamin C.

[0018] In one embodiment of the present invention, in step (3), the textile is one or more woven or knitted fabrics made of silk, wool, cotton or chemical fiber.

[0019] In one embodiment of the present invention, in step (3), the mass ratio of the textile to the mixed solution is 1:20-100.

[0020] In one embodiment of the present invention, in step (3), the pH of the mixed solution is 7.0-7.5; and the drying condition is drying at 25-40° C. for 0.5-3 h.

[0021] The second object of the present invention is to provide a textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties obtained by the above-mentioned preparation method.

[0022] The beneficial technical effects of the present invention are:

[0023] The invention is based on the fact that glucose oxidase catalyzes glucose to produce hydrogen peroxide, which has a bactericidal effect on both bacteria and viruses. The invention utilizes a protein-selected molecular modification of glucose oxidase. The modified glucose oxidase, under the action of a reducing agent, rapidly and gently aggregates on the surface of fabrics to form a uniform coating. This coating not only effectively inhibits bacteria over a long period of time, but also rapidly catalyzes glucose in sweat to produce hydrogen peroxide, which kills bacteria quickly upon contact with sweat. Furthermore, the coating significantly improves the hydrophilicity of the fabric and can withstand multiple washing cycles.

[0024] The present invention uses EDC / NHS as a crosslinking agent and a preferred protein as a modifier to modify glucose oxidase, which is then mixed with a reducing agent solution. During this process, the secondary structure of the protein molecules modified on the glucose oxidase is destroyed, exposing hydrophobic microdomains. The molecular chains unfold to form short-range β-sheets, which guide the glucose oxidase to aggregate on the fabric surface, forming a uniform antibacterial coating.

[0025] When the fabric prepared by the present invention is worn, human sweat diffuses evenly onto the fabric surface due to the fabric's excellent hydrophilicity, preventing the body from feeling damp and cold and uncomfortable. Compared with traditional methods for preparing antibacterial coatings on fabrics, the method described in the present invention has the following advantages:

[0026] (1) Environmentally friendly and harmless to the human body: Traditional nanomaterial-based photodynamic antibacterial agents are toxic to mammalian cells and can cause heavy metal pollution in water bodies; the glucose oxidase, protein, and reducing agent used in the present invention are all eco-friendly additives, harmless to the environment and the human body, and can be widely used in biology, medicine, clothing and other fields.

[0027] (2) The preparation process is mild: Bio-coupling technology selects proteins to molecularly modify glucose oxidase. The process of forming a coating on the fabric surface by the modified glucose oxidase is simple and mild, with low energy consumption and little damage to the fiber structure.

[0028] (3) Excellent antibacterial properties: Human sweat diffuses evenly onto the fabric surface through the excellent hydrophilicity of the fabric; the glucose oxidase immobilized on the fabric surface catalyzes the glucose in the sweat to produce hydrogen peroxide, further enhancing the fabric's rapid antibacterial properties (the antibacterial rate reaches 99% when sweat is added to the fabric and in contact with the fabric for 30 minutes).

[0029] (4) The present invention is simple to operate, low in cost, good in effect, and has universal applicability, and plays an important role in increasing the added value of textile products and their comprehensive utilization and development. DETAILED DESCRIPTION

[0030] The present invention is described below by way of examples. It should be understood that the examples are for the purpose of better explaining the present invention and are not intended to limit the present invention. Raw materials used in the present invention are commercially available unless otherwise specified.

[0031] Example 1:

[0032] A method for preparing a textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties, comprising the following steps:

[0033] (1) Preparation of modified glucose oxidase:

[0034] Glucose oxidase was dissolved in MES buffer solution (pH 5.5), and then EDC / NHS (EDS:NHS=1:1) was added and stirred at 25°C for 30 min to prepare solution a; the concentration of glucose oxidase in solution a was 20 μmol / L, and the concentration of EDC / NHS was 40 μmol / L;

[0035] Lysozyme was dissolved in MES buffer solution (pH 5) to prepare solution b; the concentration of lysozyme in solution b was 100 μmol / L;

[0036] An equal volume of solution b was added dropwise to solution a and stirred at 4°C in the dark for 8 h. Finally, after the reaction, the solution was dialyzed in deionized water at 4°C for 48 h using a dialysis membrane with a molecular weight cutoff of 20 kDa. The remaining solid product was freeze-dried at -30°C for 48 h to obtain the modified glucose oxidase.

[0037] (2) Preparation of antibacterial coating:

[0038] The modified glucose oxidase was dissolved in Tris-HCl buffer (pH 7.2) to prepare a 10 g / L solution, and thiourea dioxide was dissolved in Tris-HCl buffer (pH 7.2) to prepare a 40 mmol / L solution. The wool knitted fabric was placed flat in a solution of equal volumes of the above two solutions, with a bath ratio of 1:30, and reacted at 37°C for 4 hours. Finally, the fabric was taken out and dried at 35°C to obtain the textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties.

[0039] Example 2:

[0040] A method for preparing a textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties, comprising the following steps:

[0041] (1) Preparation of modified glucose oxidase:

[0042] Glucose oxidase was dissolved in MES buffer solution (pH 4.5), and then EDC / NHS (EDS:NHS=1:1) was added and stirred at 35°C for 30 min to prepare solution a; the concentration of glucose oxidase in solution a was 40 μmol / L, and the concentration of EDC / NHS was 80 μmol / L;

[0043] Dissolve bovine serum albumin in MES buffer solution (pH 5) to prepare solution b; the concentration of bovine serum albumin in solution b is 100 μmol / L;

[0044] An equal volume of solution b was added dropwise to solution a and stirred at 2°C in the dark for 16 h. Finally, after the reaction, the solution was dialyzed in deionized water at 4°C for 48 h using a dialysis membrane with a molecular weight cutoff of 20 kDa. The remaining solid product was freeze-dried at -40°C for 48 h to obtain the modified glucose oxidase.

[0045] (2) Preparation of antibacterial coating:

[0046] The modified glucose oxidase was dissolved in Tris-HCl buffer (pH 7.4) to prepare a 5 g / L solution, and tris(2-carboxyethyl)phosphine hydrochloride was dissolved in Tris-HCl buffer (pH 7.4) to prepare a 20 mmol / L solution. Pure cotton woven fabric was placed flat in a solution of equal volumes of the above two solutions, with a bath ratio of 1:50, and reacted at 35°C for 6 hours. Finally, the fabric was taken out and dried at 35°C to obtain the textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties.

[0047] Example 3:

[0048] A method for preparing a textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties, comprising the following steps:

[0049] (1) Preparation of modified glucose oxidase:

[0050] Glucose oxidase was dissolved in MES buffer solution (pH 5), and then EDC / NHS (EDS:NHS=1:1) was added and stirred at 25°C for 30 min to prepare solution a; the concentration of glucose oxidase in solution a was 50 μmol / L, and the concentration of EDC / NHS was 100 μmol / L;

[0051] Lysozyme was dissolved in MES buffer solution (pH 5) to prepare solution b; the concentration of lysozyme in solution b was 120 μmol / L;

[0052] An equal volume of solution b was added dropwise to solution a and stirred at 6°C in the dark for 12 h. Finally, after the reaction, the solution was dialyzed in deionized water at 4°C for 48 h using a dialysis membrane with a molecular weight cutoff of 20 kDa. The remaining solid product was freeze-dried at -20°C for 48 h to obtain the modified glucose oxidase.

[0053] (2) Preparation of antibacterial coating:

[0054] The modified glucose oxidase was dissolved in Tris-HCl buffer (pH 6.5) to prepare an 8 g / L solution, and L-cysteine ​​was dissolved in Tris-HCl buffer (pH 6.5) to prepare a 60 mmol / L solution. The polyester non-woven fabric was placed flat in a solution of equal volumes of the above two solutions, with a bath ratio of 1:30, and reacted at 37°C for 8 hours. Finally, the fabric was taken out and dried at 40°C to obtain the textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties.

[0055] Example 4:

[0056] A method for preparing a textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties, comprising the following steps:

[0057] (1) Preparation of modified glucose oxidase:

[0058] Glucose oxidase was dissolved in MES buffer solution (pH 5.5), and then EDC / NHS (EDS:NHS=1:1) was added and stirred at 25°C for 30 min to prepare solution a; the concentration of glucose oxidase in solution a was 20 μmol / L, and the concentration of EDC / NHS was 40 μmol / L;

[0059] Lysozyme was dissolved in MES buffer solution (pH 5) to prepare solution b; the concentration of lysozyme in solution b was 100 μmol / L;

[0060] An equal volume of solution b was added dropwise to solution a and stirred at 4°C in the dark for 8 h. Finally, after the reaction, the solution was dialyzed in deionized water at 4°C for 48 h using a dialysis membrane with a molecular weight cutoff of 20 kDa. The remaining solid product was freeze-dried at -40°C for 48 h to obtain the modified glucose oxidase.

[0061] (2) Preparation of antibacterial coating:

[0062] The modified glucose oxidase was dissolved in Tris-HCl buffer (pH 7.2) to prepare a 10 g / L solution, and thiourea dioxide was dissolved in Tris-HCl buffer (pH 7.2) to prepare a 40 mmol / L solution. The wool knitted fabric was placed flat in a solution of equal volumes of the above two solutions, with a bath ratio of 1:30, and reacted at 37°C for 4 hours. Finally, the fabric was taken out and dried at 35°C to obtain the textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties.

[0063] (3) The fabric with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties prepared in step (2) is subjected to 10 cycles of water washing.

[0064] Example 5:

[0065] A method for preparing a textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties, comprising the following steps:

[0066] (1) Preparation of modified glucose oxidase:

[0067] Glucose oxidase was dissolved in MES buffer solution (pH 4.5), and then EDC / NHS (EDS:NHS=1:1) was added and stirred at 35°C for 30 min to prepare solution a; the concentration of glucose oxidase in solution a was 40 μmol / L, and the concentration of EDC / NHS was 80 μmol / L;

[0068] Dissolve bovine serum albumin in MES buffer solution (pH 5) to prepare solution b; the concentration of bovine serum albumin in solution b is 120 μmol / L;

[0069] An equal volume of solution b was added dropwise to solution a and stirred at 2°C in the dark for 16 h. Finally, after the reaction, the solution was dialyzed in deionized water at 4°C using a dialysis membrane with a molecular weight cutoff of 20 kDa for 48 h. The remaining solid product was freeze-dried at -30°C for 48 h to obtain the modified glucose oxidase.

[0070] (2) Preparation of antibacterial coating:

[0071] The modified glucose oxidase was dissolved in Tris-HCl buffer (pH 7.4) to prepare a 5 g / L solution, and tris(2-carboxyethyl)phosphine hydrochloride was dissolved in Tris-HCl buffer (pH 7.4) to prepare a 20 mmol / L solution. Pure cotton woven fabric was placed flat in a solution of equal volumes of the above two solutions, with a bath ratio of 1:50, and reacted at 35°C for 6 hours. Finally, the fabric was taken out and dried at 35°C to obtain the textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties.

[0072] (3) The fabric with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties prepared in step (2) is subjected to 10 cycles of water washing.

[0073] Comparative Example 1:

[0074] A method for preparing an antibacterial textile comprises the following steps:

[0075] Same as Example 1, except that the amount of glucose oxidase added in step (1) was adjusted to 0 μmol / L.

[0076] Comparative Example 2:

[0077] A method for preparing an antibacterial textile comprises the following steps:

[0078] The same as Example 1, except that the amount of thiourea dioxide added in step (2) is adjusted to 0 mmol / L.

[0079] Comparative Example 3:

[0080] A method for preparing an antibacterial textile comprises the following steps:

[0081] Same as Example 1, except that the wool knitted fabric is not subjected to any treatment.

[0082] Comparative Example 4:

[0083] A method for preparing an antibacterial textile comprises the following steps:

[0084] Same as Example 2, except that the amount of bovine serum albumin added in step (1) is adjusted to 0 μmol / L.

[0085] Comparative Example 5:

[0086] A method for preparing an antibacterial textile comprises the following steps:

[0087] Same as Example 3, except that the amount of glucose oxidase added in step (1) was adjusted to 0 μmol / L.

[0088] Comparative Example 6:

[0089] A method for preparing an antibacterial textile comprises the following steps:

[0090] Same as Example 3, except that the polyester non-woven fabric is not treated in any way.

[0091] The antibacterial rate of textiles against Escherichia coli was determined according to GB / T 20944.3-2008. Long-term antibacterial activity is measured after 18-24 hours of contact with bacteria in the absence of sweat. A separate test measures the rapid antibacterial rate after 30 minutes of contact with or without sweat. Using a contact angle meter, a drop of simulated sweat was applied to the test fabric, and the time it took for the sweat to completely spread and wet the fabric was measured.

[0092] Table 1 shows the antibacterial properties of different textiles

[0093] sample Long-term antibacterial rate% Rapid antibacterial rate (sweat)% Rapid antibacterial rate (no sweat)% Wetting time s Example 1 88 99 <70 1 Example 2 81 99 <70 1 Example 3 85 99 <70 1 Example 4 75 90 <70 8 Example 5 73 88 <70 6 Comparative Example 1 87 <70 <70 1 Comparative Example 2 <70 <70 <70 >300 Comparative Example 3 0 0 0 >300 Comparative Example 4 0 <70 0 1 Comparative Example 5 80 <70 <70 1 Comparative Example 6 0 0 0 >300

[0094] Note: The wetting time is recorded as 300s. If it exceeds 300s, the fabric is recorded as non-hydrophilic.

[0095] From Table 1 we can see that:

[0096] (1) The textiles obtained by the method of the present invention (Examples 1, 2, and 3) exhibited excellent long-term antibacterial properties, with significant rapid antibacterial performance (99%) under sweat catalysis. The hydrophilicity of originally hydrophobic wool and polyester fabrics was significantly improved. The successful modification of the glucose oxidase molecule was verified, indicating that the modified glucose oxidase was successfully immobilized on the fabric surface, catalyzing glucose in sweat to produce hydrogen peroxide.

[0097] (2) The samples without any treatment (Comparative Example 3 and Comparative Example 6) maintained the hydrophilicity and hydrophobicity of the original fabric, and none of the samples had an antibacterial effect; the modified glucose oxidase was the key part in forming the antibacterial coating to improve the wettability of the fabric.

[0098] (3) Unmodified glucose oxidase (Comparative Example 4) cannot form a biological antibacterial coating under the action of a reducing agent, so the antibacterial and hydrophilic properties of the fabric are not improved.

[0099] (4) Fabrics without glucose oxidase and only with protein coating (Comparative Example 1 and Comparative Example 5) only have long-term antibacterial properties but not rapid antibacterial properties.

[0100] (5) The reducing agent is the trigger for the self-assembly of the bio-antimicrobial agent on the fiber surface. The fabric sample (Comparative Example 2) without the addition of the reducing agent during the preparation process only had rapid antibacterial properties and long-term antibacterial properties, but neither reached the values ​​of antibacterial effect described in the test standard.

[0101] (6) The weaving method of the fabric and the type of protein (Example 1, Example 2, Example 3) do not affect the antibacterial properties of the coating.

[0102] (7) Referring to Example 4 and Example 5, after 10 cycles of water washing, the long-term antibacterial properties of the samples prepared by the method of the present invention still remained good (>70%), and the rapid antibacterial performance also remained good (>85%).

[0103] In summary, the present invention provides a textile with improved perspiration-wicking efficiency and sweat-enhancing rapid antibacterial properties. This overcomes the shortcomings of existing antibacterial fabric preparation technologies, including poor bonding fastness, environmentally unfriendly antimicrobial agents, and limited rapid bactericidal efficacy. The present invention is simple to operate, low-cost, and effective, and is suitable for most fabrics. It plays a significant role in increasing the added value of textile products and promoting their comprehensive utilization and development.

[0104] Although the present invention has been disclosed above in terms of preferred embodiments, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the claims.

Claims

1. A method for preparing a textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties, characterized in that: The method comprises the following steps: (1) Glucose oxidase was dissolved in MES buffer solution, and then a cross-linking agent was added, and the mixture was stirred at 20-40°C for 20-60 minutes to prepare solution a; protein was dissolved in MES buffer solution to prepare solution b; an equal volume of solution b was added dropwise to solution a, and the mixture was stirred in the dark at 2-6°C for 8-16 hours. After the reaction was completed, the mixture was dialyzed with deionized water at 2-6°C for 24-60 hours, and the remaining solid product was freeze-dried for 36-72 hours to prepare the modified glucose oxidase; (2) dissolving the modified glucose oxidase in Tris-HCl buffer to prepare a 1-20 g / L solution; Dissolve the reducing agent in Tris-HCl buffer to prepare a 5-100 mmol / L solution; (3) Mixing the two solutions in step (2) in equal volumes, placing the textile flat in the mixed solution, reacting at 30-40° C. for 2-8 hours, taking out the fabric and drying it, thereby obtaining the textile with improved perspiration efficiency and sweat-enhanced rapid antibacterial properties.

2. The preparation method according to claim 1, characterized in that In step (1), the pH of the MES buffer solution is 4.5-6; the concentration of glucose oxidase in solution a is 5-50 μmol / L.

3. The preparation method according to claim 1, characterized in that In step (1), the cross-linking agent is EDC / NHS, and the molar ratio of glucose oxidase to the cross-linking agent is 1:

2.

4. The preparation method according to claim 1, characterized in that In step (1), the protein is one or more of lysozyme, bovine serum albumin, α-lactalbumin, insulin, and β-lactoglobulin; and the concentration of the protein in solution b is 50-150 μmol / L.

5. The preparation method according to claim 1, characterized in that In step (1), the molecular weight cut-off of the dialysis membrane used for dialysis is 14-50 kDa; the freeze-drying conditions are: temperature -20-40°C, vacuum degree 10-20 Pa.

6. The preparation method according to claim 1, characterized in that In step (2), the pH of the Tris-HCl buffer is 6.5-7.

5.

7. The preparation method according to claim 1, characterized in that In step (2), the reducing agent is one or more of thiourea dioxide, L-cysteine, tris(2-carboxyethyl)phosphine hydrochloride, mercaptoethanol, reduced glutathione, and vitamin C.

8. The method according to claim 1, characterized in that In step (3), the textile is one or more woven or knitted fabrics made of silk, wool, pure cotton or chemical fiber.

9. The method according to claim 1, characterized in that In step (3), the pH of the mixed solution is 7.0-7.5; and the drying condition is drying at 25-40° C. for 0.5-3 h.

10. A textile with improved perspiration efficiency and sweat-enhancing rapid antibacterial properties, prepared by the preparation method according to any one of claims 1 to 9.