Cherry medicine and preparation method thereof

Abstract

The invention discloses a cherry medicine, comprising the following raw materials in parts by weight: 5-12 parts of gibberellin GA3, 5-10 parts of aminoethyl hexanoate citrate, 1-2 parts of fucoidan, 0.2-0.8 parts of phospholipase D, 80-95 parts of solvent, and 1-5 parts of a penetrant; the penetrant is composed of polyether-modified heptamethyltrisiloxane and xanthan gum in a mass ratio of 2-5:1. The four active ingredients are scientifically proportioned, combined with the effect of the penetrant, to effectively promote the growth and development of cherries, increase the fruit setting rate, enhance disease resistance, and reduce the harm of diseases to plants. Fucoidan is rich in trace elements, which can provide cherries with the required nutrients and enhance the immunity of cherry plants, thereby providing better defense against infection by pathogens. Phospholipase D can promote the synthesis and release of hormones inside plants, help regulate the growth and development of cherries, and increase their fruit setting rate.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural compound pharmaceuticals, and in particular to a pharmaceutical for cherries and a preparation method thereof. Background Art

[0002] Cherries are a cultivated variety of European sweet cherries. Cherries are one of the earliest tree fruits to reach market, earning them the nickname "the first of a hundred fruits." Their vibrant red color is not only due to iron but also to their rich antioxidant content, including anthocyanins, proanthocyanidins, lutein, and zeaxanthin. These antioxidants protect against cell damage and inflammation, playing an antioxidant and anti-aging role, preventing chronic diseases, and promoting overall health. Cherries are low in sugar, with a glycemic index of only 22. They are low in calories and contain no sodium, making them a good choice for people with diabetes and hypertension. Their high nutritional value makes them a popular choice.

[0003] Cherries are native to Europe, Asia, and North Africa, with Spain, the United States, and Chile all being important cherry-producing regions. Due to their significant market potential and high profitability, China is currently introducing cherry trees, but yields are low due to environmental and technical constraints. High rainfall and high summer temperatures can cause sweet cherries to grow excessively (flowers but no fruit), resulting in low fruit set. Cherry cultivation is susceptible to various pests and diseases, such as cherry scab, gummosis, and thrips, which negatively impact cherry yield and quality. Therefore, scientifically and effectively controlling cherry pests and diseases, and improving cherry yield and quality, is a pressing issue. Summary of the Invention

[0004] In view of this, the present invention proposes a cherry medicine to solve the above problems.

[0005] The technical solution of the present invention is achieved as follows:

[0006] A cherry medicine comprises the following raw materials in parts by weight: 5-12 parts of gibberellin GA3, 5-10 parts of aminoethyl ester citrate, 1-2 parts of fucoidan, 0.2-0.8 parts of phospholipase D, 80-95 parts of solvent, and 1-5 parts of penetrant.

[0007] Preferably, a medicine for cherries comprises the following raw materials in parts by weight: 10 parts of gibberellin GA3, 8 parts of aminoethyl ester citrate, 1.5 parts of fucoidan, 0.5 parts of phospholipase D, 90 parts of solvent, and 3 parts of penetrant.

[0008] Preferably, the phospholipase D activity is 200 U / mg.

[0009] Preferably, the penetrant is a compound of an organosilicon compound and microbial polysaccharides.

[0010] Preferably, the organosilicon compound is polyether-modified heptamethyltrisiloxane, and the microbial polysaccharide is xanthan gum.

[0011] Preferably, the mass ratio of the polyether-modified heptamethyltrisiloxane to xanthan gum is 2-5:1.

[0012] Preferably, the solvent is selected from at least one of methanol, ethanol and water.

[0013] The present invention also provides a method for preparing a cherry medicine, comprising the following steps:

[0014] (1) Weigh gibberellin GA3, aminoethyl ester citrate, fucoidan, and phospholipase D according to weight, mix with solvent, and homogenize at room temperature for 30-40 minutes at a speed of 1000-2000 r / min to obtain a suspension;

[0015] (2) Add polyether-modified heptamethyltrisiloxane and xanthan gum to the suspension, and then shear at a speed of 80-100 r / min for 35-45 min to obtain a cherry agent.

[0016] The present invention also provides a method for using the cherry medicine, comprising the following steps:

[0017] S1. Dilution: Stir the cherry agent with 30-50 times of water until dissolved;

[0018] S2. With the cherry tree as the center, dig a trench on a circumference of 100-150 cm around the tree, evenly apply the cherry medicine into the trench, cover the medicine with soil, and then water until the soil is completely soaked;

[0019] S3. Spray the pesticide on the leaves of cherry trees. In the early flowering period, spray the above pesticide 1-2 times under sunny conditions. In the early fruiting period, spray 2-3 times under sunny conditions, with an interval of 4-6 days between each spraying.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] (1) The present invention provides a cherry agent, which scientifically mixes gibberellins, aminoethyl ester citrate, fucoidan, and phospholipase D, and combines the effect of a penetrant to effectively promote the growth and development of cherries, increase the fruit setting rate, enhance disease resistance, and reduce the harm of diseases to plants.

[0022] (2) The present invention provides a cherry agent, which is added with an appropriate amount of fucoidan and phospholipase D. Phospholipase D is an enzyme protein that has no direct antibacterial effect on bacteria and fungi. Its main function is to catalyze the hydrolysis of phospholipid molecules to produce phosphatidic acid and choline, thereby participating in the metabolism and signal transduction process of the cell membrane. Phospholipase D in cherries can indirectly affect the resistance of cherries to pathogenic microorganisms by regulating the growth, development and defense mechanism of cherries themselves; fucoidan has a good inhibitory effect on a variety of pathogenic bacteria and fungi of cherries.

[0023] (3) The present invention provides a cherry pesticide, wherein the penetrant is a combination of polyether-modified heptamethyltrisiloxane and xanthan gum, which can produce a synergistic effect. The polyether-modified heptamethyltrisiloxane can improve the wettability and permeability of the pesticide, while the xanthan gum can increase the adhesion of the pesticide on the plant surface. The combination of the two can better promote the absorption and penetration of the pesticide and improve the control effect. DETAILED DESCRIPTION

[0024] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0025] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods.

[0026] Unless otherwise specified, the materials, reagents, etc. used in the examples of the present invention can be obtained from commercial sources.

[0027] Example 1

[0028] A cherry medicine comprises the following raw materials in parts by weight: 5 parts of gibberellin GA3, 5 parts of aminoethyl hexanoate citrate, 1 part of fucoidan, 0.2 parts of phospholipase D, 80 parts of solvent, and 1 part of penetrant; the phospholipase D activity is 200 U / mg; the solvent is a mixture of ethanol and water, with the mass ratio of ethanol to water being 2:1; the penetrant is a mixture of polyether-modified heptamethyltrisiloxane and xanthan gum, with the mass ratio of polyether-modified heptamethyltrisiloxane to xanthan gum being 3:1.

[0029] The preparation method for the cherry medicine comprises the following steps:

[0030] (1) Weigh gibberellin GA3, aminoethyl ester citrate, fucoidan, and phospholipase D according to weight, mix with solvent, and homogenize at room temperature for 30 minutes at a speed of 1000 r / min to obtain a suspension;

[0031] (2) Add polyether-modified heptamethyltrisiloxane and xanthan gum to the suspension, and then shear at a speed of 80 r / min for 35 min to obtain a cherry agent.

[0032] Example 2

[0033] A cherry medicine comprises the following raw materials in parts by weight: 10 parts of gibberellin GA3, 8 parts of aminoethyl hexanoate citrate, 1.5 parts of fucoidan, 0.5 parts of phospholipase D, 90 parts of solvent, and 3 parts of penetrant; the phospholipase D activity is 200 U / mg; the solvent is a mixture of ethanol and water, with the mass ratio of ethanol to water being 2:1; the penetrant is a mixture of polyether-modified heptamethyltrisiloxane and xanthan gum, with the mass ratio of polyether-modified heptamethyltrisiloxane to xanthan gum being 3:1.

[0034] The preparation method for the cherry medicine comprises the following steps:

[0035] (1) Weigh gibberellin GA3, aminoethyl ester citrate, fucoidan, and phospholipase D according to weight, mix with solvent, and homogenize at room temperature for 40 minutes at a speed of 1500 r / min to obtain a suspension;

[0036] (2) Add polyether-modified heptamethyltrisiloxane and xanthan gum to the suspension, and then shear at a speed of 90 r / min for 40 min to obtain a cherry agent.

[0037] Example 3

[0038] A cherry medicine comprises the following raw materials in parts by weight: 12 parts of gibberellin GA3, 10 parts of diethylaminoethyl citrate, 2 parts of fucoidan, 0.8 parts of phospholipase D, 95 parts of solvent, and 5 parts of penetrant; the phospholipase D activity is 200 U / mg; the solvent is a mixture of methanol and water, with the mass ratio of methanol to water being 2:1; the penetrant is a mixture of polyether-modified heptamethyltrisiloxane and xanthan gum, with the mass ratio of polyether-modified heptamethyltrisiloxane to xanthan gum being 3:1.

[0039] The preparation method for the cherry medicine comprises the following steps:

[0040] (1) Weigh gibberellin GA3, aminoethyl ester citrate, fucoidan, and phospholipase D according to weight, mix with solvent, and homogenize at room temperature for 40 minutes at a speed of 2000 r / min to obtain a suspension;

[0041] (2) Add polyether-modified heptamethyltrisiloxane and xanthan gum to the suspension, and then shear at a speed of 100 r / min for 45 min to obtain a cherry agent.

[0042] Example 4

[0043] This embodiment differs from Example 2 in that the penetrant is a mixture of polyether-modified heptamethyl trisiloxane and xanthan gum, with the mass ratio of polyether-modified heptamethyl trisiloxane to xanthan gum being 2:1. Specifically, a cherry treatment agent comprises the following raw materials in parts by weight: 10 parts gibberellin GA3, 8 parts diethylaminoethyl citrate, 1.5 parts fucoidan, 0.5 parts phospholipase D, 90 parts solvent, and 3 parts penetrant; the phospholipase D activity is 200 U / mg; the solvent is a mixture of ethanol and water in a mass ratio of 2:1; and the penetrant is a mixture of polyether-modified heptamethyl trisiloxane and xanthan gum in a mass ratio of 2:1.

[0044] The preparation method of the cherry medicine is the same as that in Example 2.

[0045] Example 5

[0046] This embodiment differs from Example 2 in that the penetrant is a mixture of polyether-modified heptamethyl trisiloxane and xanthan gum, with the mass ratio of polyether-modified heptamethyl trisiloxane to xanthan gum being 5:1. This embodiment provides a cherry treatment agent comprising the following raw materials in parts by weight: 10 parts gibberellin GA3, 8 parts diethylaminoethyl citrate, 1.5 parts fucoidan, 0.5 parts phospholipase D, 90 parts solvent, and 3 parts penetrant; the phospholipase D activity is 200 U / mg; the solvent is a mixture of ethanol and water in a mass ratio of 2:1; and the penetrant is a mixture of polyether-modified heptamethyl trisiloxane and xanthan gum in a mass ratio of 5:1.

[0047] The preparation method of the cherry medicine is the same as that in Example 2.

[0048] Comparative Example 1

[0049] This example provides a cherry agent, whose components and preparation method are generally the same as those of Example 2, except that this example does not contain fucoidan, and the missing amount of fucoidan is distributed to the corresponding components according to the ratio of gibberellin, aminoethyl ester citrate, and phospholipase D, that is, a cherry agent comprising the following raw materials in parts by weight: 10.8 parts of gibberellin GA3, 8.6 parts of aminoethyl ester citrate, 0.6 parts of phospholipase D, 90 parts of solvent, and 3 parts of penetrant; the phospholipase D enzyme activity is 200 U / mg; the solvent is a mixture of ethanol and water, with a mass ratio of ethanol to water of 2:1; the penetrant is a mixture of polyether-modified heptamethyltrisiloxane and xanthan gum, with a mass ratio of polyether-modified heptamethyltrisiloxane to xanthan gum of 3:1.

[0050] Comparative Example 2

[0051] This example provides a cherry medicine, whose components and preparation method are generally the same as those of Example 2, except that this example does not contain fucoidan, and the missing amount of fucoidan is distributed to the corresponding components according to the ratio of gibberellin, aminoethyl ester citrate, and phospholipase D, that is, a cherry medicine, comprising the following raw materials in parts by weight: 10.3 parts of gibberellin GA3, 8.2 parts of aminoethyl ester citrate, 1.5 parts of fucoidan, 90 parts of solvent, and 3 parts of penetrant; the phospholipase D enzyme activity is 200 U / mg; the solvent is a mixture of ethanol and water, and the mass ratio of ethanol to water is 2:1; the penetrant is a mixture of polyether-modified heptamethyltrisiloxane and xanthan gum, and the mass ratio of polyether-modified heptamethyltrisiloxane to xanthan gum is 3:1.

[0052] Comparative Example 3

[0053] This example provides a medicine for cherries, the components and preparation method of which are substantially the same as those in Example 2, except that in this example, fucoidan is replaced with astragalus polysaccharide.

[0054] Comparative Example 4

[0055] This example provides a medicine for cherries, the components and preparation method of which are substantially the same as those in Example 2, except that phospholipase D is replaced with phospholipase A in this example.

[0056] Comparative Example 5

[0057] This example provides a cherry agent, whose components and preparation method are generally the same as those in Example 2, except that the penetrant in this example is polyether-modified heptamethyltrisiloxane, that is, a cherry agent comprising the following raw materials in parts by weight: 10 parts of gibberellin GA3, 8 parts of aminoethyl ester citrate, 1.5 parts of fucoidan, 0.5 parts of phospholipase D, 90 parts of solvent, and 3 parts of polyether-modified heptamethyltrisiloxane; the phospholipase D activity is 200 U / mg; the solvent is a mixture of ethanol and water, with a mass ratio of ethanol to water of 2:1.

[0058] Comparative Example 6

[0059] This example provides a cherry agent, whose components and preparation method are generally the same as those in Example 2, except that the penetrant in this example is xanthan gum, that is, a cherry agent comprising the following raw materials in parts by weight: 10 parts of gibberellin GA3, 8 parts of aminoethyl ester citrate, 1.5 parts of fucoidan, 0.5 parts of phospholipase D, 90 parts of solvent, 90 parts of solvent, and 3 parts of xanthan gum; the phospholipase D activity is 200 U / mg; the solvent is a mixture of ethanol and water, and the mass ratio of ethanol to water is 2:1.

[0060] The above Examples 1 to 5 and Comparative Examples 1 to 6 were all used in accordance with the following method:

[0061] S1. Dilution: Stir the above-mentioned cherry agent with 40 times water until dissolved;

[0062] S2. With the cherry tree as the center, dig a trench on a 120 cm circumference of the tree, evenly apply the cherry medicine into the trench, cover the medicine with soil, and then water until the soil is completely soaked;

[0063] S3. Spray the pesticide on the leaves of cherry trees. In the early flowering period, spray the above pesticide twice under sunny conditions. In the early fruiting period, spray the pesticide twice under sunny conditions, with an interval of 5 days between each spraying.

[0064] Test Example 1

[0065] The test site was a cherry orchard in Nanping City, Fujian Province. The cherry variety tested was Santina. The experimental field was flat and had medium and uniform fertility. 180 cherry trees of similar growth and age were selected. The trees were divided into a control group and 11 test groups (corresponding to the cherry agents obtained in Examples 1-5 and Comparative Examples 1-6), with 15 cherry trees in each group. The control group was treated with an equal amount of water as a control. The specific application method is: S1. Dilution: Stir the above-mentioned cherry agent with 40 times of clean water until it dissolves; S2. With the cherry tree as the center, dig a trench on a circumference of 120 cm around the tree, and evenly apply the cherry agent into the trench. Cover the agent with soil, and then water until the soil is completely soaked; S3. Spray the agent on the leaves of the cherry trees. In the early flowering period, spray the above-mentioned agent twice under sunny conditions, and in the early fruiting period, spray it twice under sunny conditions, with an interval of 5 days each time.

[0066] During the cultivation period, the disease status of cherry plants was observed and recorded. The symptoms of cherry scab are as follows: round or irregular dark brown lesions will appear on the infected fruit. The surface of the lesions is sunken and sometimes cracked. Small dark brown spots may appear on the infected leaves, and the edges of the spots may be yellow or red. Infected flowers may develop brown or black lesions. The symptoms of cherry gummosis are divided into two types: dry rot and ulcer gummosis. Dry rot mostly occurs on the main trunk and branches. The early lesions are irregular, dark brown, and have a hard surface, which often causes gummosis. Later, the lesions are long, shrunken and sunken, sometimes cracked around the edges, and densely covered with small black spots. In the ulcer gummosis, resin is produced in the diseased part of the tree, but it does not flow out immediately. Instead, it is retained between the xylem and phloem. The diseased part is slightly raised and flows out from the lenticels or wounds in the diseased part along with the sap. When the fruits were ripe and harvested, their fruit set rate and deformity rate were calculated. In addition, 50 cherries were randomly selected from each group and their soluble sugar content was tested using UV spectrophotometry. The statistical results of the experiment are shown in Table 1.

[0067] Table 1

[0068]

[0069] It can be seen from the results in Table 1 that the agent for cherries provided in Examples 1-5 of the present invention can significantly increase the fruit setting rate and soluble sugar content of cherries, effectively reduce the rate of deformed fruits, and effectively improve the disease resistance of cherries. The incidence of cherry black spot and cherry gummosis are significantly reduced. Comparative Examples 1-2 omitted fucoidan and phospholipase D from the agent, respectively, and their disease resistance was significantly weakened. Comparative Examples 3-4 replaced fucoidan and phospholipase D with other ingredients, which had a certain impact on their synergistic effect. Fucoidan is rich in trace elements, which can provide the required nutrients for cherries and enhance the immunity of cherry plants, so that they can better defend against infection by pathogens. Phospholipase D can promote the synthesis and release of hormones inside plants, help regulate the growth and development of cherries, and increase their fruit setting rate. Comparative Examples 5-6 replaced the penetrant in the agent with a single ingredient, which affected the crop's absorption of the agent components. The combined use of polyether-modified heptamethyltrisiloxane and xanthan gum can produce a synergistic effect. Polyether-modified heptamethyltrisiloxane can improve the wettability and permeability of the pesticide, while xanthan gum can increase the adhesion of the pesticide on the plant surface. The combination of the two can better promote the absorption and penetration of the pesticide and improve the prevention and control effect.

[0070] Test Example 2

[0071] The pathogens of infectious cherry gummosis are primarily fungi, such as Botrytis cinerea, Phomopsis caudatus, and Fusarium oxysporum. Some bacteria, such as Pseudomonas syringae, can also induce gummosis. The antibacterial properties of the cherry preparations prepared in Example 2 and Comparative Examples 1-6 were tested using the Oxford cup method (tube-and-plate method), a common method for determining antibiotic potency both domestically and internationally. The inhibition zone test was used to determine the inhibitory effects of the test samples on Botrytis cinerea (BNCCBNCC121059), Fusarium oxysporum (ATCC48112), and Pseudomonas syringae (ATCC39254).

[0072] Test sample: 200 mg of each of the cherry medicines prepared in Example 2 and Comparative Examples 1-6 were diluted with sterile deionized water to prepare a test solution with a solid content of 25 mg / ml. The following test was performed: Aseptically, the high-temperature sterilized culture medium was poured into sterile culture dishes, so that each culture dish contained 20 mL of culture medium. After cooling, 1 mL of activated Botrytis cinerea, Fusarium oxysporum, and Pseudomonas syringae (concentration of 1.5×10 7 / mL), evenly spread on the surface of the culture medium, 8 culture dishes for each bacteria (corresponding to 7 test solutions and negative controls), 3 Oxford cups were placed above the culture dishes, and the same test solution was injected into the 3 Oxford cups as three parallels. Sterile water was used as a negative control. 0.2mL of each test sample was added to each small tube, and the cells were placed in a 37°C incubator for anaerobically incubated for 24 hours. The diameter of the inhibition zone in each group of culture dishes was measured after 24 hours and the average value was taken. The antibacterial effect was judged as follows: an inhibition zone diameter greater than 20mm was considered high efficiency; an inhibition zone diameter of 15-20mm was considered medium efficiency; an inhibition zone diameter of 10-15mm was considered low efficiency; and an inhibition zone diameter less than 10mm was considered ineffective. The results are shown in Table 2 below.

[0073] Table 2

[0074] Group Botrytis cinerea Fusarium oxysporum Pseudomonas syringae Example 2 21.2 22.6 20.3 Comparative Example 1 12.1 11.0 10.9 Comparative Example 2 20.1 21.2 19.8 Comparative Example 3 18.3 20.2 18.6 Comparative Example 4 11.8 11.4 10.5 Comparative Example 5 18.5 18.0 16.9 Comparative Example 6 18.8 20.5 18.6 Negative control / / /

[0075] As can be seen from Table 1, the cherry agent prepared in Example 2 of the present invention has a good inhibitory effect on a variety of pathogenic bacteria; the results of comparative examples 1-4 show that phospholipase D is an enzyme protein and has no direct antibacterial effect on bacteria and fungi. Its main function is to catalyze the hydrolysis of phospholipid molecules to produce phosphatidic acid and choline, thereby participating in the metabolism and signal transduction process of the cell membrane. Phospholipase D in cherries can indirectly affect the resistance of cherries to pathogenic microorganisms by regulating the growth and development and defense mechanisms of cherries themselves; fucoidan has a good inhibitory effect on a variety of pathogenic bacteria and fungi of cherries; the inhibitory effect of the cherry agent prepared in comparative examples 5-6 on pathogenic bacteria is weakened to a certain extent, indicating that the composition of the penetrant has a great influence on the antibacterial effect.

[0076] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A medicine for cherries, characterized in that: The invention comprises the following raw materials in parts by weight: 5-12 parts of gibberellin GA3, 5-10 parts of diethylaminoethyl citrate, 1-2 parts of fucoidan, 0.2-0.8 parts of phospholipase D, 80-95 parts of solvent, and 1-5 parts of penetrant. The penetrant is a compound of polyether-modified heptamethyltrisiloxane and xanthan gum, and the mass ratio of the two is 2-5:

1.

2. The agent for cherries according to claim 1, characterized in that: The preparation comprises the following raw materials in parts by weight: 10 parts of gibberellin GA3, 8 parts of diethylaminoethyl citrate, 1.5 parts of fucoidan, 0.5 parts of phospholipase D, 90 parts of solvent and 3 parts of penetrant.

3. The agent for cherries according to claim 1, characterized in that: The solvent is selected from at least one of methanol, ethanol and water.

4. The method for preparing a medicine for cherries according to any one of claims 1 to 3, characterized in that: The steps include: (1) Weigh gibberellin GA3, aminoethyl ester citrate, fucoidan, and phospholipase D according to weight, mix with solvent, and homogenize at room temperature for 30-40 minutes at a speed of 1000-2000 r / min to obtain a suspension; (2) Add polyether-modified heptamethyltrisiloxane and xanthan gum to the suspension, and then shear at a speed of 80-100 r / min for 35-45 minutes to obtain the cherry agent.

5. The method for using the cherry medicine according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1. Dilution: Stir the cherry agent with 30-50 times water until dissolved; S2. With the cherry tree as the center, dig a trench on a circumference of 100-150 cm around the tree, evenly apply the cherry medicine into the trench, cover the medicine with soil, and then water until the soil is completely soaked; S3. Spray the pesticide on the leaves of cherry trees. In the early flowering period, spray the above pesticide 1-2 times under sunny conditions. In the early fruiting period, spray 2-3 times under sunny conditions, with an interval of 4-6 days between each spraying.

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