A multi-almond powder for helping to maintain a healthy level of blood lipids and a preparation method thereof

Abstract

The application discloses a multi-hemp seed powder for helping to maintain a healthy level of blood lipid and a preparation method thereof. Fire hemp seeds, flax seeds and sesame seeds are compounded in a ratio of 35-40:20-30:35-40, and then are crushed, mixed, subjected to low-water fermentation, subjected to high-pressure pulse electric field sterilization and enzyme inactivation, subjected to emulsion self-assembly conditioning, and subjected to freeze-drying treatment. The low-water fermentation of the prepared coarse powder realizes the adjustment of the nutritional components and the sensory characteristics of the hemp seed powder, and has a detoxification effect. The product prepared by the application has a fat content of up to 40%, rich in unsaturated fatty acids alpha-linolenic acid and linoleic acid, and has a unique oil flavor of hemp seeds. The ratio of linoleic acid and alpha-linolenic acid in the multi-hemp seed powder is close to 4:1, which is the best ratio recognized by the World Health Organization for human body. The unsaturated fatty acids, hemp cannabinoids, lignans and other substances have a synergistic effect and are used for regulating the blood lipid level of the human body.

Description

Technical Field

[0001] This invention belongs to the field of food processing technology. Background Technology

[0002] Obesity caused by a high-fat diet can lead to many physiological changes, such as gut microbiota dysbiosis, causing various metabolic diseases including hypertension, diabetes, intestinal inflammation, cardiovascular disease, and cancer. In daily diet, low lipid metabolism levels and excessive fat intake can cause lipid accumulation in the liver and cardiovascular system, leading to obesity. Therefore, regulating blood lipid levels through a proper daily diet is a good way to improve obesity.

[0003] Hemp seed is a classic food and medicine source, with unsaturated fatty acids accounting for over 90% of its composition. Besides trace elements like vitamin B, alkaloids, and flavonoids, it also contains cannabinoids as a natural byproduct, giving it various physiological activities such as antioxidation, metabolism regulation, lipid-lowering, and cardiovascular protection. Lignans, one of the main active ingredients in flaxseed and sesame, can lower blood pressure and lipids, and have anti-inflammatory, anti-thrombotic, and anti-atherosclerotic effects. In addition to its significant physiological functions, sesame oil contains heterocyclic compounds such as pyrazines, pyrroles, pyridines, thiazoles, thiophenes, and furans, which give it a pleasant aroma. Therefore, flaxseed oil and other similar products have been widely marketed due to their strong nutritional value; however, the presence of cyanogenic glycosides in flaxseed limits its direct use as a common food. Unsaturated fatty acids have various activities such as lowering blood pressure and enhancing immunity, and the ratio of linoleic acid to linolenic acid is close to 4:1, which is the optimal ratio for the human body recognized by the World Health Organization. These unsaturated fatty acids, along with a variety of active substances such as cannabinoids, lignans, sterols, tocopherols, flavonoids, phytosterols, saponins, and flavonoids, have strong antioxidant activity, anti-inflammatory effects, and can lower blood lipids, blood pressure, and cholesterol. Their synergistic effect can have a beneficial impact on the body's lipid metabolism.

[0004] To better utilize the nutritional components of flaxseed, sesame, and hemp, patent CN108477574A discloses a method for preparing defatted flaxseed powder and its uses. This method involves detoxifying and defatting flaxseed powder and preparing composite nanoparticles. While this method improves the nutritional value of flaxseed powder, the resulting powder lacks beneficial lipid components, and the taste and aroma of the single ingredient are poor. Patent CN114128863A discloses a reconstituted cereal powder and its preparation method. Most commercially available oil-rich cereal powders are single or combined reconstituted powders made from typical oilseed crops such as soybeans, peanuts, and sesame. These products have poor solubility, lack a smooth and delicate texture, and their nutritional components are not prominent. Their fatty acid composition is mostly saturated fatty acids, resulting in poor health benefits. Furthermore, as solid beverages, thickeners and emulsifiers are often added to achieve higher viscosity, reducing nutritional value. Some existing technologies also use fermentation to prepare related products. For example, patent CN109735460B discloses a method for detoxifying flaxseed cake through solid-state fermentation using compound microorganisms. This method removes most of the cyanogenic glycosides through solid-state fermentation, followed by complete removal through high-pressure treatment. However, using only oil-removed flaxseed cake as raw material and subjecting it to high-temperature, high-pressure drying reduces the trace beneficial substances and aromatic compounds in the fermented product, resulting in the incomplete utilization of the beneficial components in flaxseed. Summary of the Invention

[0005] To address the aforementioned problems, the purpose of this invention is to prepare a multi-flavor sesame seed powder that helps maintain healthy blood lipid levels, solving issues such as poor solubility, unpleasant taste, and high saturated fatty acid content in existing instant powders. This invention achieves a 1:4 to 1:6 ratio of linolenic acid (ω-3 fatty acid) to linoleic acid (ω-6 fatty acid) in the multi-flavor sesame seed powder through compounding of basic raw materials. The synergistic effects of linolenic acid, linoleic acid, lignans, and cannabinoids can regulate blood lipids. Simultaneously, an appropriate amount of flaxseed gum imparts a certain degree of adhesiveness, and the sesame flavor enhances palatability. Low-water fermentation improves the taste and absorbability of the food, while high-voltage pulsed electric field sterilization detoxifies and sterilizes the raw materials, thereby increasing the removal rate of cyanogenic glycosides.

[0006] To achieve the above objectives, the present invention first provides a method for preparing hemp seed powder that helps maintain healthy blood lipid levels, comprising the following steps:

[0007] (1) Select hemp seeds, flax seeds and sesame seeds as basic materials and mix them together to obtain 100-150 mesh pre-made coarse powder under the action of a high-speed universal pulverizer;

[0008] (2) Mix the fermentation agent, purified water and the pre-made coarse powder obtained in step (1), stir evenly, and place the mixture in a constant temperature incubator for low-water sealed fermentation. The fermentation agent includes 0.5% to 1.5% dry yeast and 1% to 3% enzyme preparation by weight of the pre-made coarse powder.

[0009] (3) The fermented powder obtained after fermentation is sterilized and inactivated by high voltage pulse electric field;

[0010] (4) Take the baking powder obtained in step (3), add pure water, and perform high-pressure homogenization through two homogenizers to obtain multi-sesame seed powder emulsion;

[0011] (5) The emulsion obtained in step (4) is dried to obtain multi-hemp seed powder which helps maintain healthy blood lipid levels.

[0012] The low-water fermentation described in this invention refers to the growth and product formation of microorganisms on solid particles under conditions of no free water (or near-no free water), while maintaining the water content of the system within 40%.

[0013] In one embodiment of the present invention, in step (1), the basic raw materials, calculated by mass, include 35-40 parts of hemp seeds, 20-30 parts of flax seeds, and 35-40 parts of sesame seeds.

[0014] In one embodiment of the present invention, in step (2), the mixture comprises 100 parts coarse powder, 40 to 60 parts purified water, and 1 to 2 parts fermentation agent.

[0015] In one embodiment of the present invention, in step (2), the yeast is selected from at least one of brewer's yeast, Hansenbali yeast, and wine yeast.

[0016] In one embodiment of the present invention, the yeast is a mixture of brewer's yeast, Hansenbali yeast, and wine yeast.

[0017] In one embodiment of the present invention, the enzyme preparation is selected from at least one of neutral protease, acidic protease, and papain.

[0018] In one embodiment of the present invention, the enzyme preparation is a mixture of neutral protease, acidic protease, and papain.

[0019] In one embodiment of the present invention, in step (2), the fermentation temperature is 30-40 °C, the fermentation time is 48-72 h, and the mixture is turned over once every 24 h during the fermentation period.

[0020] In one embodiment of the present invention, during the high-voltage pulsed electric field sterilization process in step (3), the field strength is 15–30 kV·cm. -1The fixed pulse frequency was 40 Hz, the material flow rate was 15 mL / min, the pulse width was 2 μs, the ambient temperature was 24 ℃, and the pulse treatment time was 50–150 s.

[0021] In one embodiment of the present invention, in step (4), the volume ratio of yeast to purified water is 1:1 to 1:2, and the homogenization pressure is 30-35 MPa.

[0022] In one embodiment of the present invention, in step (5), before drying, the hemp seed powder emulsion is placed in a pre-freezing chamber at -80°C and frozen for 30 minutes, and then vacuum freeze-drying is performed to remove moisture.

[0023] In one embodiment of the present invention, during vacuum freeze drying, the material thickness is 6-12 mm, the pressure is 50-100 Pa, the heating plate temperature is 50-70 °C, and the heating time is 30-60 min.

[0024] The present invention also discloses a multi-hemp seed powder prepared according to the above method.

[0025] This invention also discloses an application of the above-mentioned multi-sesame seed powder in the food industry.

[0026] Beneficial effects of the present invention

[0027] (1) Rich in nutrients and full of flavor. In the whole fat multi-flavor sesame powder of the present invention and its preparation method and application, the product made by the present invention has a fat content of up to 40% (rich in unsaturated fatty acids α-linolenic acid and linoleic acid), and has the unique oily aroma of sesame seeds.

[0028] (2) The ratio of linoleic acid to linolenic acid in sesame seed powder is close to 4:1, which is the optimal ratio for human needs recognized by the World Health Organization. These unsaturated fatty acids, along with substances rich in cannabinoids, lignans, etc., work synergistically to regulate the body's blood lipid levels.

[0029] (3) The compounded hemp seeds are crushed in advance to enhance the dispersibility and flowability of the raw materials, so that they are fully mixed before subsequent processing.

[0030] (4) Novel production process. Conventional grain flour is obtained only through grain crushing, which is simple to process, but the processed product contains natural toxins and has low nutritional value. Through low-water fermentation, under the action of microbial detoxification technology, the content of cyanogenic glycosides in the product is reduced, the protein quality is improved, and the content of beneficial metabolites produced by yeast, active peptides and other probiotics is increased, which can improve immunity. The method of this invention has high efficiency in removing toxins such as cyanogenic glycosides, low production cost, high yield and good economic benefits.

[0031] (5) Novel food-derived bioactive peptides are prepared by microbial and enzymatic hydrolysis to improve nutritional quality and digestibility.

[0032] (6) Compared with traditional pasteurization heat technology, high-voltage pulse electric field sterilization causes less damage to food and has a stronger lethal effect on microorganisms such as brewer's yeast. It enhances product safety, has little impact on nutrients (especially vitamins), and the equipment is easy to operate, has controllable speed, low energy consumption, and can achieve continuous processing, enabling it to expand its processing capacity to realize industrial production and continuous production combined with other technologies.

[0033] (7) Conventional grain powder is obtained only by crushing grains. After the oil is broken down, it is easily oxidized and rancid when exposed to air, making it difficult to store and consume for a long time. Self-assembled composite nanoparticles have good biocompatibility and biodegradability, and can be used as carriers for fatty acids and active substances to encapsulate or transport them, thereby effectively improving the utilization rate and stability of bioactive components.

[0034] (8) Vacuum freeze drying is a drying process carried out in a low temperature and vacuum environment, which has a good protective effect on the sensory characteristics and nutritional components of the material and is suitable for heat-sensitive and easily oxidized products.

[0035] (9) The present invention provides a formula and preparation method for a multi-flavored sesame seed powder that helps maintain healthy blood lipid levels. The full-fat multi-flavored sesame seed powder produced by the invention can be consumed directly as a solid beverage or mixed with water, or it can be added as an ingredient to a variety of existing health foods to fully utilize the nutritional value of the raw materials and has broad application prospects. Attached Figure Description

[0036] Figure 1 This is a flowchart of the invention;

[0037] Figure 2 The effects of different diets on blood lipid levels in SD rats;

[0038] Figure 3 These are the data on the cannabinoid, lignan, cyanide, and polyphenol content of the hemp seed powder prepared in the examples and comparative examples. Detailed Implementation

[0039] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be described in detail below. Obviously, the described embodiments are merely some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other implementation methods obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0040] The neutral protease, acidic protease, and papain involved in the embodiments and comparative examples of this invention all have an enzyme activity of 100,000 u / g.

[0041] Sensory evaluation testing methods:

[0042] A sensory evaluation panel of 10 professionally trained personnel was selected to evaluate the product's aroma, color, solubility, taste, and overall quality, with each item scored out of 10. The sesame seed powder was prepared by mixing it with hot water at 70℃ at a solid-liquid ratio of 1:5. Specific scoring items and standards are shown in the table.

[0043]

[0044] Content detection: The content of cannabinoids and polyphenols in the sesame seed powder was detected by LC-MS / MS; the content of lignans in the sesame seed powder was detected by liquid chromatography; and the content of cyanide in the sesame seed powder was detected according to the detection method of GB5009.36-2023.

[0045] Example 1

[0046] A method for preparing multi-sesame seed powder that helps maintain healthy blood lipid levels includes the following steps:

[0047] Step 1: Take 35 parts of hemp seeds, 30 parts of flax seeds, and 35 parts of sesame seeds, mix them together, and then use a high-speed universal grinder to obtain pre-made coarse powder in the range of 100-150 mesh.

[0048] Step 2: Take 1.5% of the pre-made coarse powder by weight of W38 brewer's yeast and 3% of neutral protease, mix them to obtain the starter culture, take 100 parts coarse powder, 50 parts purified water, and 1 part starter culture, mix them and stir evenly, and place them in a constant temperature incubator for sealed fermentation. The fermentation temperature is 30 ℃ and the fermentation time is 72 h. During the fermentation, stir once every 24 h to obtain the fermented powder.

[0049] Step 3: Place the fermentation powder in a high-voltage pulsed electric field for sterilization and enzyme inactivation treatment, with an electric field strength of 15 kV·cm. -1 Meanwhile, the pulse frequency was fixed at 40 Hz, the material flow rate at 15 mL / min, the pulse width at 2 μs, the ambient temperature at 24 ℃, and the pulse processing time at 150 s.

[0050] Step 4: Mix the sterilized fermentation powder with purified water at a volume ratio of 1:1, and then perform high-pressure homogenization using two homogenizers at a pressure of 30-35 MPa to obtain a multi-sesame seed powder emulsion.

[0051] Step 5: Pre-cool the freezer for 30 minutes, add the sesame seed powder emulsion, spread it evenly to a thickness of 6 mm, and quickly freeze it to -80 ℃ within 30 minutes. Set the vacuum freeze-drying process to a pressure of 50 Pa and a heating plate temperature of 70 ℃. The heating plate is heated for 60 minutes. Monitor the freeze-drying temperature and material weight changes in real time. When the temperature and weight change curves tend to be constant, the drying endpoint is reached, and a sesame seed powder that helps maintain healthy blood lipid levels is obtained.

[0052] Example 2

[0053] A method for preparing multi-sesame seed powder that helps maintain healthy blood lipid levels includes the following steps:

[0054] Step 1: Take 40 parts of hemp seeds, 20 parts of flax seeds, and 40 parts of sesame seeds, mix them together, and then use a high-speed universal grinder to obtain pre-made coarse powder in the range of 100-150 mesh.

[0055] Step 2: Take 0.5% W38 brewer's yeast, 0.5% Y52 Hansen's barley yeast, 1% acidic protease and 0.5% papain by weight of the pre-made coarse powder, mix them to obtain the starter culture. Take 100 parts coarse powder, 40 parts purified water and 1 part starter culture, mix them and stir evenly, and place them in a constant temperature incubator for sealed fermentation. The fermentation temperature is 30 ℃ and the fermentation time is 72 hours. During the fermentation, stir once every 24 hours to obtain the fermented powder.

[0056] Step 3: Place the fermentation powder in a high-voltage pulsed electric field for sterilization and enzyme inactivation treatment, with an electric field strength of 20 kV·cm. -1 Meanwhile, the pulse frequency was fixed at 40 Hz, the material flow rate at 15 mL / min, the pulse width at 2 μs, the ambient temperature at 24 ℃, and the pulse processing time at 100 s.

[0057] Step 4: Mix the sterilized fermentation powder with purified water at a volume ratio of 1:2, and then perform high-pressure homogenization using two homogenizers at a pressure of 30-35 MPa to obtain a multi-sesame seed powder emulsion.

[0058] Step 5: Pre-cool the freezer for 30 minutes, add the sesame seed powder emulsion, spread it evenly to a thickness of 9 mm, and quickly freeze it to -80 ℃ within 30 minutes. Set the vacuum freeze-drying process to a pressure of 75 Pa and a heating plate temperature of 60 ℃. The heating plate is heated for 30 minutes, and the freeze-drying temperature and material weight changes are monitored in real time. When the temperature and weight change curves tend to be constant, the drying endpoint is reached, and the sesame seed powder that helps maintain healthy blood lipid levels is obtained.

[0059] Example 3

[0060] A method for preparing multi-sesame seed powder that helps maintain healthy blood lipid levels includes the following steps:

[0061] Step 1: Take 40 parts of hemp seeds, 20 parts of flax seeds, and 40 parts of sesame seeds, mix them together, and then use a high-speed universal grinder to obtain pre-made coarse powder in the range of 100-150 mesh.

[0062] Step 2: Take 0.5% of W38 brewer's yeast, 0.5% of Y52 Hansen's barley yeast, 0.5% of 71B wine yeast and 1.5% of neutral protease by weight of the pre-made coarse powder, mix them to obtain the starter culture. Take 100 parts coarse powder, 50 parts purified water and 1 part starter culture, mix them and stir evenly, and place them in a constant temperature incubator for sealed fermentation. The fermentation temperature is 30 ℃ and the fermentation time is 72 h. During the fermentation, stir once every 24 h to obtain the fermented powder.

[0063] Step 3: Place the fermentation powder in a high-voltage pulsed electric field for sterilization and enzyme inactivation treatment, with an electric field strength of 25 kV·cm. -1 Meanwhile, the pulse frequency was fixed at 40 Hz, the material flow rate at 15 mL / min, the pulse width at 2 μs, the ambient temperature at 24 ℃, and the pulse processing time at 75 s.

[0064] Step 4: Mix the sterilized fermentation powder with purified water at a volume ratio of 1:1, and then perform high-pressure homogenization using two homogenizers at a pressure of 30-35 MPa to obtain a multi-sesame seed powder emulsion.

[0065] Step 5: Pre-cool the freezer for 30 minutes, add the sesame seed powder emulsion, spread it evenly to a thickness of 12 mm, and quickly freeze it to -80 ℃ within 30 minutes. Set the vacuum freeze-drying process to 100 Pa pressure and 50 ℃ heating plate temperature. The heating plate is heated for 30 minutes, and the freeze-drying temperature and material weight changes are monitored in real time. When the temperature and weight change curves tend to be constant, the drying endpoint is reached, and the sesame seed powder that helps maintain healthy blood lipid levels is obtained.

[0066] Example 4

[0067] A method for preparing multi-sesame seed powder that helps maintain healthy blood lipid levels includes the following steps:

[0068] Step 1: Take 35 parts of hemp seeds, 30 parts of flax seeds, and 35 parts of sesame seeds, mix them together, and then use a high-speed universal grinder to obtain pre-made coarse powder in the range of 100-150 mesh.

[0069] Step 2: Take 0.5% of W38 brewer's yeast, 0.5% of Y52 Hansen's barley yeast, 0.5% of 71B wine yeast, and 1% of neutral protease, 0.5% of acidic protease, and 0.5% of papain by weight of the pre-prepared coarse powder, mix them to obtain the starter culture. Take 100 parts coarse powder, 50 parts purified water, and 1 part starter culture, mix them thoroughly, and place them in a constant temperature incubator for sealed fermentation. The fermentation temperature is 30 ℃, and the fermentation time is 72 h. During the fermentation, the mixture is turned over once every 24 h to obtain the fermented powder.

[0070] Step 3: Place the fermentation powder in a high-voltage pulsed electric field for sterilization and enzyme inactivation treatment, with an electric field strength of 30 kV·cm. -1 Meanwhile, the pulse frequency was fixed at 40 Hz, the material flow rate at 15 mL / min, the pulse width at 2 μs, the ambient temperature at 24 ℃, and the pulse processing time at 50 s.

[0071] Step 4: Mix the sterilized fermentation powder with purified water at a volume ratio of 1:2, and then perform high-pressure homogenization using two homogenizers at a pressure of 30-35 MPa to obtain a multi-sesame seed powder emulsion.

[0072] Step 5: Pre-cool the freezer for 30 minutes, add the sesame seed powder emulsion, spread it evenly to a thickness of 9 mm, and quickly freeze it to -80 ℃ within 30 minutes. Set the vacuum freeze-drying process to a pressure of 70 Pa and a heating plate temperature of 65 ℃. The heating plate is heated for 30 minutes, and the freeze-drying temperature and material weight changes are monitored in real time. When the temperature and weight change curves tend to be constant, the drying endpoint is reached, and the sesame seed powder that helps maintain healthy blood lipid levels is obtained.

[0073] Comparative Example 1

[0074] A method for preparing multi-sesame seed powder that helps maintain healthy blood lipid levels includes the following steps:

[0075] Step 1: Take 100 parts of sesame seeds and use a high-speed universal grinder to obtain pre-made coarse powder in the range of 100-150 mesh.

[0076] Step 2: Take 0.5% of W38 brewer's yeast, 0.5% of Y52 Hansen's barley yeast, 0.5% of 71B wine yeast, and 1% of neutral protease, 0.5% of acidic protease, and 0.5% of papain, which are based on the mass of the pre-prepared coarse powder. Mix them to obtain the starter culture. Take 100 parts of coarse powder, 50 parts of purified water, and 1 part of starter culture. Mix them thoroughly and place them in a constant temperature incubator for sealed fermentation. The fermentation temperature is 30 ℃ and the fermentation time is 72 h. During the fermentation, stir once every 24 h to obtain the fermented powder.

[0077] Step 3: Place the fermentation powder in a high-voltage pulsed electric field for sterilization and enzyme inactivation treatment, with an electric field strength of 30 kV·cm. -1 Meanwhile, the pulse frequency was fixed at 40 Hz, the material flow rate at 15 mL / min, the pulse width at 2 μs, the ambient temperature at 24 ℃, and the pulse processing time at 50 s.

[0078] Step 4: Mix the sterilized fermentation powder with purified water at a volume ratio of 1:2, and then perform high-pressure homogenization using two homogenizers at a pressure of 30-35 MPa to obtain a multi-sesame seed powder emulsion.

[0079] Step 5: Pre-cool the freezer for 30 minutes, add the sesame seed powder emulsion, spread it evenly to a thickness of 9 mm, and quickly freeze it to -80 ℃ within 30 minutes. Set the vacuum freeze-drying process to a pressure of 70 Pa and a heating plate temperature of 65 ℃. The heating plate is heated for 30 minutes, and the freeze-drying temperature and material weight changes are monitored in real time. When the temperature and weight change curves tend to be constant, the drying endpoint is reached, and the sesame seed powder that helps maintain healthy blood lipid levels is obtained.

[0080] Comparative Example 2

[0081] A method for preparing multi-sesame seed powder that helps maintain healthy blood lipid levels includes the following steps:

[0082] Step 1: Take 35 parts of hemp seeds, 30 parts of flax seeds, and 35 parts of sesame seeds, mix them together, and then use a high-speed universal grinder to obtain pre-made coarse powder in the range of 100-150 mesh.

[0083] Step 2: The pre-prepared coarse powder is placed in a high-voltage pulsed electric field for sterilization and enzyme inactivation treatment, with an electric field strength of 30 kV·cm. -1 Meanwhile, the pulse frequency was fixed at 40 Hz, the material flow rate at 15 mL / min, the pulse width at 2 μs, the ambient temperature at 24 ℃, and the pulse processing time at 50 s.

[0084] Step 3: Mix the sterilized powder with purified water at a volume ratio of 1:2, and then perform high-pressure homogenization using two homogenizers at a pressure of 30-35 MPa to obtain a multi-sesame seed powder emulsion.

[0085] Step 4: Pre-cool the freezer for 30 minutes, add the sesame seed powder emulsion, spread it evenly to a thickness of 9 mm, and quickly freeze it to -80 ℃ within 30 minutes. Set the vacuum freeze-drying process to a pressure of 70 Pa and a heating plate temperature of 65 ℃. The heating plate is heated for 30 minutes, and the freeze-drying temperature and material weight changes are monitored in real time. When the temperature and weight change curves tend to be constant, the drying endpoint is reached, and the sesame seed powder that helps maintain healthy blood lipid levels is obtained.

[0086] Comparative Example 3

[0087] A method for preparing multi-sesame seed powder that helps maintain healthy blood lipid levels includes the following steps:

[0088] Step 1: Take 35 parts of hemp seeds, 30 parts of flax seeds, and 35 parts of sesame seeds, mix them together, and then use a high-speed universal grinder to obtain pre-made coarse powder in the range of 100-150 mesh.

[0089] Step 2: Take 0.5% of W38 brewer's yeast, 0.5% of Y52 Hansen's barley yeast, 0.5% of 71B wine yeast, and 1% of neutral protease, 0.5% of acidic protease, and 0.5% of papain by weight of the pre-prepared coarse powder, mix them to obtain the starter culture. Take 100 parts coarse powder, 50 parts purified water, and 1 part starter culture, mix them thoroughly, and place them in a constant temperature incubator for sealed fermentation. The fermentation temperature is 30 ℃, and the fermentation time is 72 h. During the fermentation, the mixture is turned over once every 24 h to obtain the fermented powder.

[0090] Step 3: Place the fermentation powder in a high-voltage pulsed electric field for sterilization and enzyme inactivation treatment, with an electric field strength of 30 kV·cm. -1 Meanwhile, the pulse frequency was fixed at 40 Hz, the material flow rate at 15 mL / min, the pulse width at 2 μs, the ambient temperature at 24 ℃, and the pulse processing time at 50 s.

[0091] Step 4: Mix the sterilized fermentation powder with purified water at a volume ratio of 1:2, and then perform high-pressure homogenization using two homogenizers at a pressure of 30-35 MPa to obtain a multi-sesame seed powder emulsion.

[0092] Step 5: Dry the sesame seed powder emulsion using a 70 ℃ hot air dryer. Monitor the changes in drying temperature and material weight in real time. When the temperature and weight change curves tend to be constant, the drying endpoint is reached, and sesame seed powder that helps maintain healthy blood lipid levels is obtained.

[0093] Comparative Example 4

[0094] A method for preparing multi-sesame seed powder that helps maintain healthy blood lipid levels includes the following steps:

[0095] Step 1: Take 35 parts of hemp seeds, 30 parts of flax seeds, and 35 parts of sesame seeds, mix them together, and then use a high-speed universal grinder to obtain pre-made coarse powder in the range of 100-150 mesh.

[0096] Step 2: Take 0.5% of W38 brewer's yeast, 0.5% of Y52 Hansen's barley yeast and 0.5% of 71B wine yeast by weight of the pre-made coarse powder and mix them to obtain the starter culture. Take 100 parts coarse powder, 50 parts purified water and 1 part starter culture, mix them and stir evenly, and place them in a constant temperature incubator for sealed fermentation. The fermentation temperature is 30 ℃ and the fermentation time is 72 h. During the fermentation, stir once every 24 h to obtain the fermented powder.

[0097] Step 3: Place the fermentation powder in a high-voltage pulsed electric field for sterilization and enzyme inactivation treatment, with an electric field strength of 30 kV·cm. -1 Meanwhile, the pulse frequency was fixed at 40 Hz, the material flow rate at 15 mL / min, the pulse width at 2 μs, the ambient temperature at 24 ℃, and the pulse processing time at 50 s.

[0098] Step 4: Mix the sterilized fermentation powder with purified water at a volume ratio of 1:2, and then perform high-pressure homogenization using two homogenizers at a pressure of 30-35 MPa to obtain a multi-sesame seed powder emulsion.

[0099] Step 5: Pre-cool the freezer for 30 minutes, add the sesame seed powder emulsion, spread it evenly to a thickness of 9 mm, and quickly freeze it to -80 ℃ within 30 minutes. Set the vacuum freeze-drying process to a pressure of 70 Pa and a heating plate temperature of 65 ℃. The heating plate is heated for 30 minutes, and the freeze-drying temperature and material weight changes are monitored in real time. When the temperature and weight change curves tend to be constant, the drying endpoint is reached, and the sesame seed powder that helps maintain healthy blood lipid levels is obtained.

[0100] Animal experiments:

[0101] Forty SD rats, weighing approximately 180 g, were randomly divided into two groups after one week of acclimatization: a blank control group (n=10) and a high-fat group (n=30). The blank control group was fed a basal diet, while the high-fat group was fed a basal diet supplemented with lard, cholesterol, and sodium cholate (basal diet 87.7%, cholesterol 2%, sodium cholate 0.3%, lard 10%). After four weeks of feeding, with 12 hours of fasting but no water restriction, blood was collected from the tail to measure the serum concentrations of TC, TG, HDL-C, and LDL-C. After confirming successful modeling, the high-fat group was divided into three groups of 10 animals each, for a total of four groups: blank control group, high-fat model control group, Example 4 multi-hemp seed powder intervention group, and Comparative Example 1 multi-hemp seed powder intervention group. The blank control group was fed a basal diet, the high-fat model control group was fed a high-fat diet for four weeks and then a basal diet, the Example 4 multi-hemp seed powder intervention group was fed a high-fat diet for four weeks and then a diet supplemented with Example 4 multi-hemp seed powder, and the Comparative Example 1 multi-hemp seed powder intervention group was fed a high-fat diet for four weeks and then a diet supplemented with Comparative Example 1 multi-hemp seed powder.

[0102] During the experiment, the temperature (22±2 ℃) and humidity (50%-60%) of the rat room were controlled, and the SD rats were allowed free access to water and food for 6 consecutive weeks. After fasting for 12 hours but with free access to water, blood was collected from the tail of the SD rats to measure the serum concentrations of TC, TG, HDL-C, and LDL-C.

[0103] The indicators were measured by centrifuging serum at 4000 r / min for 5 min in a 4 ℃ centrifuge and then immediately placing it in a -80 ℃ freezer. Serum lipid levels (TC, TG, LDL-C, HDL-C) were measured according to the instructions of the cholesterol (TC) assay kit, triglyceride (TG) assay kit, low-density lipoprotein cholesterol (LDL-C) assay kit, and high-density lipoprotein cholesterol (HDL-C) assay kit purchased from Beyotime Biotechnology Co., Ltd.

[0104] Animal experimental data showed that, through testing the blood lipid levels of SD rats fed a high-fat diet for a long period, their blood lipid levels were significantly higher than those of rats fed a normal diet, accompanied by obvious symptoms of hyperlipidemia, thus confirming the successful establishment of a hyperlipidemia model. By intervening in the diet of rats with hyperlipidemia symptoms with hemp seed powder, compared with the model control group, the serum total cholesterol, total triglycerides, and low-density lipoprotein levels in the hemp seed powder intervention group (Example 4) were significantly reduced. Although the various indices in the hemp seed powder intervention group (Comparative Example 1) were somewhat alleviated, they were still significantly higher than those in the normal diet group. Figure 2 As shown.

[0105] Table 1 Sensory evaluation scores of the multi-hemp seed powder of the present invention with blood lipid regulating function

[0106]

[0107] Table 1 shows the sensory evaluation results of the multi-sesame seed powders in Examples 1-4 and Comparative Examples 1-4. As can be seen from Table 1, when using a single yeast and a single enzyme for low-water fermentation, the resulting multi-sesame seed powder had lower scores in terms of aroma, color, solubility, and taste, but a higher overall score. However, when using a combination of yeasts and enzymes for fermentation, the resulting multi-sesame seed powder exhibited better aroma, color, solubility, and taste, and also had a higher overall score. The sesame seed powder prepared using only sesame seeds had poor color, solubility, and taste. The low-water fermentation method significantly affected the aroma, color, solubility, and taste scores of the multi-sesame seed powder. Although low-water fermentation with yeast could improve the sensory evaluation, the improvement in aroma and color was minimal, and the overall score remained low. The drying method of the multi-sesame seed powder also affected its aroma and color to some extent.

[0108] Figure 3The data on the cannabinoid, lignan, cyanide, and polyphenol content of the hemp seed powder prepared in Examples 4 and Comparative Examples 2-4 are shown. Figure 3 It can be seen that neither low-water fermentation without it nor low-water fermentation using only yeast can completely remove toxins such as cyanide. Conventional drying methods will greatly reduce the content of cannabinoids, lignans, and polyphenols.

[0109] The embodiments provided above are not intended to limit the scope of the invention, nor are the described steps intended to limit the order of execution. Any obvious modifications made to the invention by those skilled in the art based on existing common knowledge also fall within the scope of protection defined by the claims.

Claims

1. A method for preparing multi-hemp seed powder that helps maintain healthy blood lipid levels, characterized in that, Includes the following steps: (1) Select hemp seeds, flax seeds and sesame seeds as basic materials and mix them together to obtain 100-150 mesh pre-made coarse powder under the action of a high-speed universal pulverizer; (2) Mix the fermentation agent, purified water and the pre-made coarse powder obtained in step (1), stir evenly, and place the mixture in a constant temperature incubator for low-water sealed fermentation. The fermentation agent includes 0.5% to 1.5% of dry yeast and 1% to 3% of enzyme preparation by weight of pre-made coarse powder. The enzyme preparation is a combination of neutral protease, acidic protease and papain. (3) The fermented powder obtained after fermentation is sterilized and inactivated by high voltage pulse electric field; (4) Take the baking powder obtained in step (3), add pure water, and perform high-pressure homogenization through two homogenizers to obtain multi-sesame seed powder emulsion; (5) The emulsion obtained in step (4) is dried to obtain multi-hemp seed powder which helps maintain healthy blood lipid levels.

2. The preparation method according to claim 1, characterized in that, In step (1), the basic raw materials, calculated by mass, include 35-40 parts of hemp seeds, 20-30 parts of flax seeds, and 35-40 parts of sesame seeds.

3. The preparation method according to claim 1, characterized in that, In step (2), the mixture comprises 100 parts coarse powder, 40 to 60 parts purified water, and 1 to 2 parts fermentation agent.

4. The preparation method according to claim 1, characterized in that, In step (2), the yeast is selected from at least one of brewer's yeast, Hansenbali yeast, and wine yeast. The fermentation temperature is 30-40 °C, the fermentation time is 48-72 h, and the yeast is turned over once every 24 h during fermentation.

5. The preparation method according to claim 1, characterized in that, In step (3), during the high-voltage pulsed electric field sterilization treatment, the field strength is 15–30 kV·cm. -1 The fixed pulse frequency was 40 Hz, the material flow rate was 15 mL / min, the pulse width was 2 μs, the ambient temperature was 24 ℃, and the pulse treatment time was 50–150 s.

6. The preparation method according to claim 1, characterized in that, In step (4), the volume ratio of baking powder to purified water is 1:1 to 1:2, and the homogenization pressure is 30-35 MPa.

7. The preparation method according to claim 1, characterized in that, In step (5), before drying, the hemp seed powder emulsion is placed in a pre-freezing chamber at -80℃ and frozen for 30 minutes, and then vacuum freeze-drying is performed to remove moisture.

8. The preparation method according to claim 7, characterized in that, During vacuum freeze drying, the material thickness is 6–12 mm, the pressure is 50–100 Pa, the heating plate temperature is 50–70 ℃, and the heating time is 30–60 min.

9. The hemp seed powder prepared by the method according to any one of claims 1 to 8.

10. The application of the sesame seed powder according to claim 9 in the food industry.

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