A plant A alcohol wrinkle-removing and firming composition suitable for sensitive skin and its application

Abstract

The invention discloses a plant A alcohol anti-wrinkle and firming composition suitable for sensitive skin and its application, belonging to the technical field of cosmetics; the anti-wrinkle and firming composition provided by the invention comprises the following components by mass: 1-10 parts of Pseudostellariae cyclotide or Pseudostellariae saponin, 5-20 parts of rattan tea extract, 1-15 parts of velvet spatholobi extract, and 1-10 parts of plant A alcohol. The anti-wrinkle and firming composition provided by the invention can effectively resist photoaging, efficiently inhibit the activity of UV-induced matrix metalloproteinases, promote the synthesis of elastin, and resist oxidation for a long time, so as to achieve the effect of anti-wrinkle and firming skin; at the same time, it can also brighten the skin tone, control oil secretion, and be friendly to sensitive skin. It can be widely used in the preparation of cosmetics.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a plant A alcohol wrinkle-removing and firming composition suitable for sensitive skin and an application thereof. Background Art

[0002] Skin is the largest and most superficial organ of the human body and undergoes a complex and cumulative aging process throughout life. Of the ultraviolet (UV) radiation from the sun, UVA (long wave 320-400nm) and UVB (medium wave 280-320nm) are harmful to our skin and can cause problems such as pigmentation, premature aging, edema, erythema, hyperplasia, etc. One of the hallmarks of photoaging is the aggregation of dystrophic elastin, which causes an imbalance in the extracellular matrix.

[0003] Elastin is a protein present in several connective tissues and has unique physiological elasticity. Elastin is synthesized by fibroblasts and keratinocytes to provide support for blood vessels, lungs and skin. Reduced elastin synthesis and functional damage lead to loss of elasticity, resulting in sagging skin, prone to wrinkles and fat masses. Microfibrils can serve as a "scaffold" for the aggregation of tropoelastin (a soluble elastin precursor) and play an important supporting role. They are composed of components such as fibrillins, fibrobulins, microfibril-associated glycoproteins (MAGPs) and proteoglycans. After elastin is synthesized and released from cells, it aligns the cross-linked domains of microfibrils, aggregates and precipitates on this "scaffold", regulates the maturation of elastin, and microfibrils profoundly affect the biological properties of elastin. However, increased expression of matrix metalloproteinase 1 (MMP-1) reduces the synthesis of elastin and microfibrils, which is a characteristic detection indicator of photoaged skin.

[0004] The prior art provides a lot of anti-aging active substances, but most of the anti-aging active substances provided by the prior art are mainly based on exogenous collagen supplementation, and the research on endogenous anti-photoaging is not comprehensive and the effect is not ideal. Furthermore, after UVB irradiates the skin, the framework of its skin fibers is one of the structures most seriously affected by it. Its degradation leads to the gradual loss of tissue support, thereby affecting the appearance. There is a lack of a composition that effectively exerts its good biological functions, such as improving the accumulation of abnormal elastin in the extracellular space, structural degradation of elastic fibers, wrong elastic fiber orientation, and protection of the dermis. In addition, most of the anti-aging compositions provided by the prior art fail to balance high efficiency and mildness, and still have irritation problems. They are not suitable for people with sensitive skin, which limits the scope of product use. Summary of the invention

[0005] The purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a plant A alcohol anti-wrinkle and firming composition suitable for sensitive skin, which can effectively resist photoaging, thereby achieving excellent wrinkle removal and skin firming effects, and also has certain oil control and skin brightening effects and its application.

[0006] To achieve the above objectives, in a first aspect of the present invention, the present invention provides an anti-wrinkle and firming composition, which comprises the following components in parts by weight: 1-10 parts of Pseudostellaria caerulea cyclic peptide or Pseudostellaria caerulea saponin, 5-20 parts of Rhizoma Cyclostridium Officinale extract, 1-15 parts of Pseudostellaria aviculare extract, and 1-10 parts of Phytophthora Adenophora.

[0007] The wrinkle-removing and firming composition provided by the present invention has excellent synergistic effects among the components by selecting four components in a suitable mass ratio range, can effectively resist photoaging, efficiently solve the problem of skin photoaging, and prevent and improve skin aging, thereby achieving the effect of firming the skin; at the same time, the four components can also effectively brighten the skin tone and reduce oil secretion by synergizing within a specific mass ratio range; and the components provided by the present invention are mild and non-irritating, and are suitable for sensitive skin.

[0008] Specifically, Radix Pseudostellariae is a traditional Chinese medicine, rich in trace elements, amino acids, sugars, glycosides, cyclic peptides, fatty acids, oils, volatile oils, sterols and other active ingredients; Radix Pseudostellariae has small toxic side effects and a wide safety range; Radix Pseudostellariae cyclic peptides or Radix Pseudostellariae saponins are natural products from Radix Pseudostellariae, which have anti-inflammatory and anti-cancer effects. Rattan tea is the tender stems and leaves of the genus Agaricus in the family Vitaceae. It has long been used as tea and as a Chinese herbal medicine. Due to its rich content of flavonoids and polyphenolic compounds, it has antibacterial, antiviral, anti-tumor, anti-inflammatory and anti-inflammatory functions. The combination of Rattan tea and Radix Pseudostellariae extracts can achieve anti-inflammatory and antioxidant synergy, resist oxidative stress, regulate immunity, effectively inhibit the degradation of skin fibers caused by UV, and regulate skin oil secretion, thereby resisting and improving UV damage to the skin. As an important medicinal plant, Scutellaria baicalensis has long been used in India to treat skin diseases. When combined with plant A alcohol, it can synergistically stimulate the production of skin extracellular matrix, enhance skin elasticity, density and thickness, smooth and brighten the skin, further improve photoaging, inhibit skin wrinkles and sagging, and relieve irritation and soothe the skin.

[0009] As a preferred embodiment of the anti-wrinkle and firming composition of the present invention, the anti-wrinkle and firming composition comprises the following components in parts by weight: 2-5 parts of Pseudostellaria heterophylla cyclic peptide or Pseudostellaria heterophylla saponin, 9-14 parts of Camellia ternata extract, 3-8 parts of Scutellaria indica extract, and 2-5 parts of phytorenin.

[0010] The present invention has found that the weight percentage of the components in the wrinkle-removing and firming composition will have a significant impact on the performance of the product. When the weight percentage of the components is further selected to be within the above range, the obtained product has better wrinkle-removing, firming, anti-aging, skin-brightening and oil-control effects.

[0011] As a preferred embodiment of the wrinkle-removing and firming composition of the present invention, the Pseudostellariae cyclic peptide includes at least one of Pseudostellariae cyclic peptide AD, and the Pseudostellariae saponins include at least one of Pseudostellariae saponins AH.

[0012] Preferably, the Pseudostellariae cyclic peptide is Pseudostellariae cyclic peptide B.

[0013] Preferably, the Pseudostellariae Radix Saponins is Pseudostellariae Radix Saponins A

[0014] The present invention has found that different Pseudostellariae cyclic peptides and Pseudostellariae saponins will affect the compounding effect with other components. When the Pseudostellariae cyclic peptide is further selected as Pseudostellariae cyclic peptide AD or the Pseudostellariae saponins are Pseudostellariae saponins AH, especially when the Pseudostellariae cyclic peptide is Pseudostellariae cyclic peptide B or the Pseudostellariae saponins are Pseudostellariae saponins A, the product can be better coordinated with other components to achieve good wrinkle removal, firming, oil control and skin brightening effects.

[0015] As a preferred embodiment of the wrinkle-removing and firming composition of the present invention, the plant A alcohol includes at least one of phytol, bakuchiol, and rambutan leaf extract.

[0016] Preferably, the plant A alcohol is phytol.

[0017] Phytol is an isoprenoid alcohol that combines with chlorophyll through an ester bond. Chlorophyll is the most abundant photosynthetic pigment in plants and has anti-cancer and immune-enhancing effects. The present invention has found that when phytol is compounded with other components, it can better resist light damage, improve photoaging, inhibit the formation and sagging of skin wrinkles, increase skin elasticity and brightness, and can also better control oil.

[0018] As a preferred embodiment of the wrinkle-removing and firming composition of the present invention, the preparation method of the rattan tea extract includes the following steps: taking the rattan tea and crushing it, adding it to an ethanol aqueous solution, adding citric acid at the same time, followed by ultrasonic extraction, filtering after the extraction, collecting the filtrate and concentrating it, then adding ethanol to the concentrate for alcohol precipitation, filtering after the precipitation, collecting the solid and drying it to obtain the rattan tea extract.

[0019] Preferably, the mass percentage of ethanol in the ethanol aqueous solution is 70-80%.

[0020] Preferably, based on the mass of rattan tea, the added amount of citric acid is 0.2-0.4%, and the added amount of ethanol aqueous solution is 8-12 times.

[0021] Preferably, in ultrasonic extraction, the ultrasonic power is 100-140 W, the time is 20-40 min, the temperature is 45-55° C., and the number of extractions is 2-3 times.

[0022] Preferably, in the alcohol precipitation, the mass ratio of the concentrate to ethanol is 1:(0.8-1.2), the alcohol precipitation temperature is 30-40°C, and the alcohol precipitation time is 20-28h.

[0023] The present invention has found that there are differences in the types and contents of active ingredients obtained when the rattan tea extract is prepared by different preparation methods. When the rattan tea extract preparation method provided by the present invention is further adopted, the obtained rattan tea extract can be better compounded with other components, thereby improving the comprehensive performance of the product.

[0024] As a preferred embodiment of the wrinkle-removing and firming composition of the present invention, the preparation method of the indigofera extract comprises the following steps: taking the indigofera extract, crushing it and adding it to an ethanol aqueous solution for leaching, ultrasonically extracting it after the leaching is completed, filtering it after the extraction is completed, collecting the filtrate and concentrating and drying it to obtain the indigofera extract.

[0025] Preferably, the mass percentage of ethanol in the ethanol aqueous solution is 75-85%.

[0026] Preferably, the amount of ethanol aqueous solution added is 8-12 times the mass of Scutellaria baicalensis.

[0027] Preferably, the leaching temperature is 45-55°C, and the leaching time is 25-35 minutes.

[0028] Preferably, during ultrasonic extraction, the ultrasonic power is 250-350 W, the time is 50-70 min, and the temperature is 55-65°C.

[0029] The present invention has found that when the Herba Lysimachiae is extracted by the extraction method provided by the present invention, the Herba Lysimachiae extract obtained can better achieve the comprehensive effect of the product.

[0030] In the second aspect of the present invention, the present invention provides an anti-photoaging liposome, wherein the liposome comprises a core layer and a shell layer, wherein the core layer comprises the anti-wrinkle and firming composition of the present invention, and the shell layer comprises phospholipids and cholesterol; and the mass ratio of the core layer to the shell layer is 1:(0.5-5).

[0031] Preferably, the mass ratio of the core layer to the shell layer is 1:(1-4).

[0032] The liposome containing anti-photoaging provided by the present invention is formed into liposomes by forming the anti-wrinkle and firming composition of the present invention. The obtained liposome has a lipid bimolecular membrane structure similar to a biological membrane, has no biological toxicity and no immune activity, has good cell affinity and biocompatibility, and can achieve the corresponding effect faster in in vitro experiments; at the same time, the liposome encapsulates the anti-wrinkle and firming composition in the form of non-covalent bonds, and this encapsulation method is conducive to the slow release of the anti-wrinkle and firming composition, thereby achieving a more long-lasting effect. Furthermore, the present invention limits the mass ratio of the core layer and the shell layer to 1: (0.5-5), especially 1: (1-4), and the comprehensive effect of the obtained liposome containing anti-photoaging is better.

[0033] In the third aspect of the present invention, the present invention provides a method for preparing the liposome containing the anti-photoaging agent, the preparation method comprising the following steps:

[0034] (1) dissolving phospholipids and cholesterol in an organic solvent, and then evaporating the organic solvent to form a membrane layer;

[0035] (2) mixing the phosphate buffer containing the wrinkle-removing and firming composition of the present invention with the film layer formed in step (1) and subjecting them to a hydration reaction. After the hydration reaction is completed, ultrasonication and filtration are performed to collect the filter residue to obtain liposomes containing anti-photoaging agents.

[0036] As a preferred embodiment of the preparation method of the present invention, the mass ratio of phospholipid to cholesterol is (1.8-2.2):1.

[0037] As a preferred embodiment of the preparation method of the present invention, the phospholipid includes at least one of lecithin, soybean lecithin, and egg yolk lecithin.

[0038] As a preferred embodiment of the preparation method of the present invention, the organic solvent includes at least one of dichloromethane, chloroform, ether, ethyl acetate and chloroform.

[0039] As a preferred embodiment of the preparation method of the present invention, the volume ratio of the sum of the masses of the phospholipid and cholesterol to the organic solvent is 1 mg: (0.080-0.085) mL.

[0040] As a preferred embodiment of the preparation method of the present invention, the mass volume ratio of the wrinkle-removing and firming composition to the phosphate buffer is 1 mg: (0.8-1.2) mL.

[0041] As a preferred embodiment of the preparation method of the present invention, the temperature of the hydration reaction is 35-39° C. and the time is 25-35 min.

[0042] As a preferred embodiment of the preparation method of the present invention, the ultrasonic time is 8-12 minutes.

[0043] Further ultrasound after the hydration reaction can reduce the particle size of the formed liposomes, thereby obtaining liposomes with smaller particle size and better overall effect.

[0044] In a fourth aspect of the present invention, the present invention provides use of the wrinkle-removing and firming composition or the anti-photoaging-containing liposome in the preparation of cosmetics.

[0045] As a preferred embodiment of the application of the present invention, the cosmetics include any one of lotion, emulsion, cream, mask, essence, and spray.

[0046] In a fifth aspect of the present invention, the present invention provides a facial cream comprising the following components in percentage by mass:

[0047] 3-10% of the wrinkle-removing and firming composition or the liposome containing anti-photoaging agent, 2-40% of cosmetic base, and the balance of water.

[0048] As a preferred embodiment of the facial cream of the present invention,

[0049] As a preferred embodiment of the wrinkle-removing and firming composition of the present invention, the cosmetic matrix includes at least one of a thickener, a moisturizer, an emulsifier, a preservative, a fragrance, and a pH adjuster.

[0050] Exemplarily, the thickener includes at least one of xanthan gum, carbomer, AVC, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, and sorbitan isostearate; the moisturizer includes at least one of glycerin, butylene glycol, isononyl isononanoate, squalane, and caprylic / capric triglyceride; the emulsifier includes at least one of cetearyl olivate, sorbitan olivate, coconut oil-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, cetearyl glucoside, polyglyceryl-6 distearate, and polymethylsilsesquioxane; the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and parahydroxyacetophenone; the pH adjuster includes at least one of arginine and disodium EDTA.

[0051] Compared with the prior art, the present invention has the following beneficial effects:

[0052] (1) The present invention provides a wrinkle-removing and firming composition, which can synergize the components in anti-photoaging through scientific compounding, achieve excellent antioxidant and highly effective inhibition of UV-induced matrix metalloproteinase activity, prevent elastin degradation, effectively strengthen elastin scaffolds, and promote the aggregation and maturation of elastin on microfibrils; specifically, the wrinkle-removing and firming composition provided by the present invention can upregulate elastin genes, promote elastin synthesis, ensure the correct occurrence of skin elasticity, thereby giving the skin flexibility and ductility, avoiding epidermal flattening, fundamentally stabilizing skin elasticity, and effectively improving photoaging, skin sagging and sagging; at the same time, it can also effectively brighten the skin tone and control oil secretion;

[0053] (2) The anti-photoaging liposome provided by the present invention is formed by encapsulating the anti-wrinkle and firming composition with liposomes, and the liposomes have good cell affinity and biocompatibility, and can also effectively stabilize the anti-wrinkle and firming composition, and help release the anti-wrinkle and firming composition, so as to achieve rapid and long-lasting anti-oxidation, wrinkle removal, skin firming, skin lightening and oil control effects;

[0054] (3) When the wrinkle-removing and firming composition or the liposome containing anti-photoaging provided by the present invention is applied to users with sensitive skin, it can not only reduce expression lines, improve skin elasticity and tighten the skin, but also increase the thickness and density of the dermis, reduce oil secretion and brighten the skin; and it will not cause skin irritation, that is, it has the characteristics of safety and mildness, is suitable for sensitive skin, has a wide range of applications and is highly accepted by consumers;

[0055] (4) The anti-wrinkle and firming composition or the anti-photoaging liposome provided by the present invention can be widely used in the preparation of cosmetics, helping to achieve the preparation of mild cosmetics suitable for sensitive skin that have the effects of anti-wrinkle and firming, brightening skin tone and controlling oil. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] Figure 1 The test results of crow's feet on the left face using the cream of Application Example 1 on D0 and D28 in Effect Example 3;

[0057] Figure 2 This is a graph showing the crow's feet test results on the left face on days D0 and D28 using the cream in Comparative Application Example 1 in Effect Example 3. DETAILED DESCRIPTION

[0058] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0059] Unless otherwise specified, the reagents, methods and equipment used in the present invention are all conventional reagents, methods and equipment in the art; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0060] Tengcha extract 1: Homemade, preparation method:

[0061] Take an appropriate amount of rattan tea, wash it, put it into a 45°C constant temperature oven to dry to constant weight, grind it to obtain rattan tea powder, pass the powder through a 100-mesh sieve, collect the sieve under, and obtain rattan tea powder; add 10 times the mass of 75% ethanol aqueous solution and 0.003 times the mass of citric acid to the rattan tea powder, then extract for 30 minutes at an ultrasonic power of 120W and 50°C, repeat the extraction once, collect the filtrate by filtration and concentrate to obtain a concentrated solution, add 95% ethanol to the concentrated solution at a mass ratio of 1:1, then precipitate at 35°C for 24 hours to remove impurities, filter after the precipitation, collect the filter residue and store it in a 4°C refrigerator for later use, and obtain rattan tea extract 1;

[0062] Tengcha extract 2: Homemade, preparation method:

[0063] Take an appropriate amount of rattan tea, wash it, put it into a 45°C constant temperature oven and dry it to constant weight, grind it to obtain rattan tea powder, pass the powder through a 100-mesh sieve, collect the sieve under, and obtain rattan tea powder; add 10 times the mass of water and 0.003 times the mass of citric acid to the rattan tea powder, then ultrasonically extract for 30 minutes at an ultrasonic power of 120W and 50°C, repeat the extraction once, collect the filtrate by filtration and concentrate to obtain a concentrate, add 95% ethanol to the concentrate at a mass ratio of 1:1, then precipitate at 35°C for 24 hours to remove impurities, filter after the precipitation, collect the filter residue and store it in a 4°C refrigerator for later use, and obtain rattan tea extract 2;

[0064] Tengcha extract 3: Homemade, preparation method:

[0065] Take an appropriate amount of rattan tea, wash it, put it into a 45°C constant temperature oven and dry it to constant weight, grind it to obtain rattan tea powder, pass the powder through a 120-mesh sieve, collect the sieve under, and obtain rattan tea powder; add 15 times the mass of 60% ethanol aqueous solution and 0.003 times the mass of citric acid to the rattan tea powder, soak for 48 hours, then filter and collect the filtrate and concentrate to obtain a concentrated solution, add 95% ethanol to the concentrated solution at a mass ratio of 1:1, then precipitate at 35°C for 24 hours to remove impurities, filter after the precipitation, collect the filter residue and store it in a 4°C refrigerator for later use, and obtain rattan tea extract 3;

[0066] Extract of gentiana velutipes 1: Homemade, the preparation method is:

[0067] Wash the herb and put it into a 45°C constant temperature oven to dry to constant weight, grind and crush to obtain herb powder, pass the powder through a 120-mesh sieve, collect the sieve underflow, and obtain herb powder; add 10 times the mass of 80% ethanol aqueous solution to the herb powder, extract at 50°C for 30 minutes, and then extract at an ultrasonic power of 300W and a temperature of 60°C for 1 hour, then filter and collect the filtrate, and concentrate it by rotary evaporation at 60°C, and then place the concentrate in an ultra-low temperature freeze dryer for drying and freezing to obtain herb extract 1;

[0068] Extract of gentiana velutipes 2: Homemade, the preparation method is:

[0069] Wash the herb and put it into a 45°C constant temperature oven to dry to constant weight, grind and crush to obtain herb powder, pass the powder through a 120-mesh sieve, collect the sieved material, and obtain herb powder; add 10 times the mass of 80% ethanol aqueous solution to the herb powder, extract at 50°C for 1.5h, filter and collect the filtrate, and concentrate by rotary evaporation at 60°C, then place the concentrate in an ultra-low temperature freeze dryer for drying and freezing, and obtain herb extract 2;

[0070] Extract of gentiana velutipes 3: Homemade, the preparation method is:

[0071] Wash the Herba Lycopersicon esculentum, put it into a 45°C constant temperature oven and dry it to constant weight, grind it to obtain Herba Lycopersicon esculentum powder, pass the powder through a 120-mesh sieve, collect the sieve residue, and obtain Herba Lycopersicon esculentum powder; add 10 times the mass of 80% ethanol aqueous solution to the Herba Lycopersicon esculentum powder, extract it for 1.5 hours at an ultrasonic power of 300 W and a temperature of 60°C, then filter and collect the filtrate, and concentrate it by rotary evaporation at 60°C, then dry and freeze the concentrate in an ultra-low temperature freeze dryer to obtain Herba Lycopersicon esculentum extract 3;

[0072] Heterophyllin B: purchased from MedChemExpress, CAS No.: 145459-19-4;

[0073] Pseudostellarins A: purchased from Chengdu Pusi Biotechnology Co., Ltd., CAS No.: 156430-20-5;

[0074] Phytol: purchased from Suzhou Nakang Biotechnology Co., Ltd., with the trade name of phytol;

[0075] Rambutan leaf (Nephelium lappaceum) extract: purchased from BASF AG under the trade name Nephydrat TM .

[0076] Examples 1-9 and Comparative Examples 1-4

[0077] The present invention provides an anti-wrinkle and firming composition according to the embodiment and comparative example. The components (parts by weight) of the anti-wrinkle and firming composition are shown in Table 1.

[0078] Table 1

[0079]

[0080]

[0081] The preparation method of the wrinkle-removing and firming composition provided in Example 1 is: mixing the components evenly to obtain the wrinkle-removing and firming composition.

[0082] The preparation methods of the wrinkle-removing and firming compositions provided in Examples 2-9 and Comparative Examples 1-4 are consistent with that in Example 1.

[0083] Example 10

[0084] The embodiment of the present invention provides a wrinkle-removing and firming composition, the wrinkle-removing and firming composition being different from that of embodiment 1 in that Pseudostellariae saponin A is used instead of Pseudostellariae cyclopeptide B.

[0085] Embodiment 11

[0086] The embodiment of the present invention provides a wrinkle-removing and firming composition. The only difference between the wrinkle-removing and firming composition and the embodiment 1 is that rambutan leaf extract is used instead of phytol.

[0087] Comparative Example 5

[0088] The comparative example of the present invention provides an anti-wrinkle and firming composition, the anti-wrinkle and firming composition is different from Example 1 only in that flaxseed cyclic peptide is used instead of Pseudostellariae cyclic peptide B.

[0089] Comparative Example 6

[0090] The comparative example of the present invention provides an anti-wrinkle and firming composition, the anti-wrinkle and firming composition being different from Example 1 in that white tea extract is used instead of rattan tea extract 1.

[0091] Comparative Example 7

[0092] The comparative example of the present invention provides an anti-wrinkle and firming composition, the anti-wrinkle and firming composition is different from Example 1 only in that the Rubia cordifolia extract is used instead of the Herba Lysimachiae Indici Extract 1.

[0093] Comparative Example 8

[0094] The comparative example of the present invention provides a wrinkle-removing and firming composition, the only difference between the wrinkle-removing and firming composition and Example 1 is that retinol is used instead of phytol.

[0095] Example 12

[0096] The embodiment of the present invention provides an anti-photoaging liposome, wherein the anti-photoaging liposome comprises a core layer and a shell layer, and the mass ratio of the core layer to the shell layer is 1:1; the anti-wrinkle and firming composition in the core layer is the anti-wrinkle and firming composition provided in Example 1, and the shell layer comprises lecithin and cholesterol, and the mass ratio of lecithin to cholesterol is 2:1; the preparation method of the photoaging liposome comprises the following steps:

[0097] (1) uniformly mixing the components of the wrinkle-removing and firming composition to obtain the wrinkle-removing and firming composition;

[0098] (2) preparing an active ingredient mixed solution by adding the wrinkle-removing and firming composition to a phosphate buffer solution at a mass volume ratio of 1 mg / mL, and dissolving the solution by ultrasonication;

[0099] (3) Weigh 120 mg of phospholipids and cholesterol in a mass ratio of 2:1 and dissolve them in 10 mL of chloroform. Transfer the mixture to a round-bottom flask and dissolve them in a water bath with ultrasonic until the solution becomes transparent. Rotate and evaporate the organic solvent at 40°C to form a transparent film on the inner side of the flask. Blow away the residual organic solvent with nitrogen.

[0100] (3) The solution of step (2) was then added to the round-bottom flask of step (3) and hydrated in a water bath at 37°C for 30 min. After complete demembraning, the solution was ultrasonicated for 10 min to reduce the particle size. The solution was then filtered through a 0.45 μm microporous filter membrane, and the residue was collected and dried to obtain the anti-photoaging liposomes, which were then stored in a refrigerator at 4°C for future use.

[0101] Embodiment 13

[0102] The embodiment of the present invention provides a photosensitive liposome, and the only difference between the photosensitive liposome and the embodiment 12 is that the mass ratio of the core layer to the shell layer is 1:4.

[0103] Embodiment 14

[0104] The embodiment of the present invention provides a photosensitive liposome, and the only difference between the photosensitive liposome and the embodiment 12 is that the mass ratio of the core layer to the shell layer is 1:0.5.

[0105] Embodiment 15

[0106] The embodiment of the present invention provides a photosensitive liposome, and the only difference between the photosensitive liposome and the embodiment 12 is that the mass ratio of the core layer to the shell layer is 1:5.

[0107] Application examples 1-13, comparative application examples 1-8 and blank application examples

[0108] Application Examples 1-13, Comparative Application Examples 1-8 and Blank Application Examples of the present invention provide a facial cream, the components (mass percentage) of the facial cream are shown in Table 2; wherein the anti-wrinkle and firming compositions in Application Examples 1-11 are respectively the anti-wrinkle and firming compositions prepared in Examples 1-11, and the anti-wrinkle and firming compositions in Comparative Application Examples 1-8 are respectively the anti-wrinkle and firming compositions prepared in Comparative Examples 1-8; for example, the anti-wrinkle and firming composition used in Application Example 1 is the anti-wrinkle and firming composition prepared in Example 1, the anti-wrinkle and firming composition used in Application Example 2 is the anti-wrinkle and firming composition prepared in Example 2, the anti-wrinkle and firming composition used in Comparative Application Example 1 is the anti-wrinkle and firming composition prepared in Comparative Example 1, and the same applies to the others; the anti-photoaging liposomes prepared in Example 12 are used in Application Example 12, and the anti-wrinkle and firming composition prepared in Example 1 is used in Application Example 13;

[0109] Table 2

[0110]

[0111]

[0112] Among them, some of the substances in Table 2 come from the following sources:

[0113] 1000: cetearyl olivate, sorbitan olivate, purchased from HALLSTARBEAUTY AND PERSONAL CARE SOLUTIONS COMPANY;

[0114] MONTANOV 68: cetearyl alcohol, cetearyl glucoside, purchased from SEPPIC SA;

[0115] SEPINOV WEO: Hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, sorbitan isostearate, available from SEPPIC SA.

[0116] The preparation method of the facial cream provided in Application Example 1 comprises the following steps:

[0117] (1) Put phase A into the main pot, heat it to 80°C, and homogenize it at a speed of 1000 rpm for 5 minutes until it is evenly dispersed. After the homogenization is completed, keep it warm for later use to obtain the prefabricated component A;

[0118] (2) Put phase B into the oil phase pot, heat it to 80°C, and homogenize it at a speed of 1000 rpm for 8 minutes. After the homogenization is completed, keep it warm for later use to obtain the prefabricated B component;

[0119] (3) Add phase C into the main pot and homogenize at a speed of 1000 rpm for 3 minutes; cool to 65°C, add pre-dissolved phase D and stir evenly; add pre-dissolved phase E and stir evenly; cool to 40°C, add phase F and stir evenly, add phase G and stir evenly, then discharge the mixture to obtain an anti-wrinkle and firming cream composition.

[0120] The preparation methods of the facial creams provided in the other application examples, comparative application examples, and blank application examples are consistent with that in Application Example 1, and no relevant components are added if they are not present.

[0121] Effect Example 1

[0122] The effect examples of the present invention explore the effects of the anti-wrinkle and firming compositions prepared in Examples 1-15 and Comparative Examples 1-8, and the anti-photoaging liposomes on the expression of metalloproteinase MMP-1 in fibroblasts induced by UVB, as well as the effect on the expression of elastin.

[0123] 1. Effects on the expression of metalloproteinase MMP-1 in fibroblasts induced by UVB

[0124] UVB irradiation accelerates the skin aging process by enhancing the expression of various matrix metalloproteinases (MPPs), such as MMP-1, inducing the degradation of extracellular matrix proteins, and downregulating the expression of skin fibroblasts. This part of the test includes the following steps:

[0125] (1) Experimental materials: phosphate buffered saline (PBS), Solebol, Shanghai; dimethyl sulfoxide (DMSO), Damao, Tianjin; high glucose medium 1×DMEM, Gibco; fetal bovine serum (FBS), Gibco; hyaluronidase and hyaluronic acid, Solebol; human skin fibroblasts (HSF) were purchased from the Kunming Cell Bank of the Chinese Academy of Sciences.

[0126] (2) Experimental method: Take a bottle of cells in good exponential growth phase, add 0.25% trypsin digestion solution, digest to make the adherent cells fall off, and count 1-4×10 5 The cells were plated at 4% 40 μg / mL and prepared into a cell suspension, which was then inoculated into a 96-well plate. The complete medium in the well was aspirated, PBS was added, and the well was exposed to UVB light for 15 min (irradiation intensity: 80-90 μW / cm 2 , irradiation dose: 80mJ / cm 2); the blank control group did not need irradiation. After 15 minutes of irradiation, the PBS solution was aspirated. Experimental group: add sample solution and set the sample concentration to 10μM (the sample concentration here is based on the weight of the anti-wrinkle and firming composition); UVB group: add PBS, blank control group: add PBS, and all three groups were cultured for 24 hours. Take the cell lysate and extract RNA, and set 3 parallel wells for each group. Use TRIzol method to extract total cell RNA, determine the purity and concentration of RNA, and take 1μg for reverse transcription. Use SYBR Green I fluorescent chimeric dye method to perform PCR detection on cDNA, and use 2 -ΔΔCt The relative mRNA expression was calculated by the method of normalizing the data of the blank group, and calculating mRNA (%) = Σ (mRNA expression of the sample group / average mRNA expression of the blank group) / 3*100%; the results are shown in Table 3.

[0127] 2. Impact on elastin expression test

[0128] Elastin in human skin is a protein that allows many tissues in the body to maintain their original shape after stretching and contracting, and allows the skin to return to its original shape after being squeezed. As we age, the elastin fibers in the skin will gradually break and be lost, causing wrinkles and loss of elasticity. Compared with human skin, zebrafish skin is highly similar to human skin in structure, except that the epidermis is differentiated epidermal cells and mucus layer instead of stratum corneum. The elastin gene is highly conserved. Elastin in the human body is synthesized by the Eln gene, and the corresponding gene of this gene in zebrafish is Elna. By testing whether the sample promotes the expression of the Elna gene in zebrafish, it can be judged whether the sample promotes the regeneration of elastin, helps the skin anti-wrinkle, and achieves the effect of skin firming. Many domestic and foreign cosmetics achieve skin anti-wrinkle and firming effects by promoting the regeneration of elastin. This part of the test includes the following steps:

[0129] (1) Use wild-type AB strain zebrafish (Daniorerio) from a reliable source (such as the China National Zebrafish Resource Center) for spawning tests. Parent fish should have good reproductive ability, and the best age is 6 to 12 months. Cross-crossing should be used as much as possible to maintain genetic diversity. A new batch of pure strain broodstock should be replaced after 5 generations. Brood fish should not have obvious visible signs of infection and disease, and should not have undergone drug treatment within 2 months. Before the breeding and spawning test, the broodstock should be domesticated for more than 14 days after being introduced into the laboratory. Test solution preparation: Use fish embryo culture medium to directly dissolve the test substance to prepare a sample solution. The concentration in the solution should not exceed 0.1% (the test concentration of the test substance must ensure a 10% mortality rate for zebrafish embryos, and the test concentration of the test substance is based on the weight of the wrinkle-removing and firming composition).

[0130] (2) Test grouping: Zebrafish were randomly selected and evenly distributed into well plates, with 12 zebrafish in each well. The zebrafish were irradiated with 312nm UVB (accumulated irradiation to 0.3J / cm 2 ) irradiated zebrafish, with 2.5mL of fish embryo culture solution added as UVB group, 2.5mL of fish embryo culture solution containing 0.8g / L acetyl hexapeptide-8 added as positive control, 2.5mL of sample solution with a concentration of 5g / L (the sample concentration here is based on the weight of the anti-wrinkle and firming composition) added as sample group, and no UVB treatment as blank control. Six parallels per group. Place in a 28℃±1℃ incubator and culture for 24h±1h. Collect 12 zebrafish in each well into a 1.5mL tube, remove the solution, add 0.5mL of RNAlater solution, and store frozen.

[0131] (3) RNA extraction: During the test, remove the RNAlater solution and rinse with PBS solution three times. Place in an ice bath, remove the PBS solution, and add 500μL TRIzol reagent. Homogenize the embryos with a pellet pestle and place in an ice bath for 10 minutes. Add 100μL chloroform, vortex for 1 minute, and place in an ice bath for 5 minutes. Centrifuge the sample for 20 minutes, transfer the upper layer solution to a 1.5mL centrifuge tube, add 250μL isopropanol, vortex for 1 minute, place in an ice bath for 10 minutes, and centrifuge the sample for 20 minutes. Remove all supernatant, add 500μL ethanol, vortex for 1 minute, centrifuge the sample for 5 minutes, and place in an ice bath for 10 minutes. Repeat this step three times. Remove the ethanol, centrifuge the sample for 5 minutes, and open the lid in a fume hood to air-dry the sample for 10 minutes. Add 10μL DNase / RNase-free ultrapure distilled water and heat at 55℃ for 15 minutes.

[0132] (4) cDNA synthesis: Dilute the RNA sample to 1000 ng / 7 μL with DNase / RNase-free ultrapure distilled water. Synthesize RNA into cDNA using PrimeScript RT Kit containing gDNA Eraser (Takara; Cat no. RR047A) and store frozen.

[0133] (5) Perform real-time RT-PCR: Prepare primer mix and real-time PCR master mix for each primer. Dilute the cDNA sample 10-fold with ultrapure distilled water without DNase / RNase. Add 9 μL of PCR master mix and 1 μL of diluted cDNA sample to each well of a 96-well PCR plate. Seal the 96-well PCR plate with optical film, centrifuge the plate for 5 minutes, and perform real-time PCR amplification.

[0134] (6) Data and result calculation: Real-time PCR data were collected. Ct was used as the amplification result, and the amplification amount of β-actin gene was used as the housekeeping gene.-ΔΔCt The relative expression of the elastin gene (Elna) was calculated as the test result. The data of the blank group were normalized, and mRNA (%) was calculated as follows: Σ(mRNA expression of the sample group / average mRNA expression of the blank group) / 3*100%; the results are shown in Table 3;

[0135] Table 3

[0136] MMP-1 / % Elna / % MMP-1 / % Elna / % Example 1 <![CDATA[113 ** ]]> <![CDATA[95 ** ]]> UVB Group <![CDATA[243 # ]]> <![CDATA[34 # ]]> Example 2 <![CDATA[123 ** ]]> <![CDATA[88 ** ]]> Blank group 100 100 Example 3 <![CDATA[128 ** ]]> <![CDATA[86 ** ]]> Positive Control / <![CDATA[87 ** ]]> Example 4 <![CDATA[132 * ]]> <![CDATA[83 ** ]]> Comparative Example 1 <![CDATA[228 * ]]> <![CDATA[46 * ]]> Example 5 <![CDATA[134 * ]]> <![CDATA[78 ** ]]> Comparative Example 2 <![CDATA[226 * ]]> <![CDATA[45 * ]]> Example 6 <![CDATA[148 * ]]> <![CDATA[74 * ]]> Comparative Example 3 <![CDATA[223 * ]]> <![CDATA[41 * ]]> Example 7 <![CDATA[146 * ]]> <![CDATA[76 * ]]> Comparative Example 4 <![CDATA[222 * ]]> <![CDATA[40 * ]]> Example 8 <![CDATA[144 * ]]> <![CDATA[72 * ]]> Comparative Example 5 <![CDATA[217 * ]]> <![CDATA[56 * ]]> Example 9 <![CDATA[142 * ]]> <![CDATA[73 * ]]> Comparative Example 6 <![CDATA[215 * ]]> <![CDATA[54 * ]]> Example 10 <![CDATA[126 ** ]]> <![CDATA[84 ** ]]> Comparative Example 7 <![CDATA[209 * ]]> <![CDATA[49 * ]]> Embodiment 11 <![CDATA[122 ** ]]> <![CDATA[85 ** ]]> Comparative Example 8 <![CDATA[211 * ]]> <![CDATA[47 * ]]> Example 12 <![CDATA[114 ** ]]> <![CDATA[94 ** ]]> Embodiment 13 <![CDATA[116 ** ]]> <![CDATA[93 ** ]]> Embodiment 14 <![CDATA[118 ** ]]> <![CDATA[91 ** ]]> Embodiment 15 <![CDATA[115 ** ]]> <![CDATA[90 ** ]]>

[0137] Note: Compared with the blank control group, the significance is indicated by #, and P value < 0.05 is indicated by #. Compared with the UVB group, the significance is indicated by *, and P value < 0.05 is indicated by *, P value < 0.01 is indicated by **, and P value > 0.05 is indicated by ns.

[0138] As can be seen from Table 3, the product obtained by the technical solution of the present invention has an inhibitory effect on the overexpression of MMP-1 induced by UVB, and can also inhibit the reduction of elastin induced by UVB, and the inhibitory effect is significant. However, when any one of Pseudostellariae Cyclotide B, Rhizoma Alpiniae Extract, Herba Lycopodiellae Extract, and Phytol is missing, the inhibitory effect of MMP-1 and elastin reduction of Comparative Examples 1-4 is significantly reduced, and when other functionally similar combinations are replaced, the effects obtained by Comparative Examples 5-8 are far less than the effects obtained by the technical solution of the present invention.

[0139] Effect Example 2

[0140] The effect examples of the present invention explore the long-lasting antioxidant capacity of the wrinkle-removing and firming compositions prepared in Examples 1-11 and Comparative Examples 1-8, and the liposomes containing anti-photoaging prepared in Examples 12-15. The antioxidant effect is to fight free radicals and inhibit free radical oxidation reactions. Free radicals slow down the metabolic rate of cells inside the skin, reduce the skin's ability to store moisture, and fail to lock in moisture, which in turn causes dry skin, damaged barriers, and decreased elasticity, leading to skin aging. DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) is a stable free radical, and its alcohol solution is purple. The stronger the sample's inhibition rate of DPPH, the lighter the color of the mixed solution, and the stronger the antioxidant property, which can be used as one of the indicators for antioxidant testing. The experiment includes the following steps:

[0141] (1) Solution preparation: 0.2 mM DPPH solution: Dissolve 0.001972 g DPPH in anhydrous ethanol solution, add dimethyl sulfoxide to make up to 25 mL, measure the absorbance at 517 nm and record the absorbance value between 200-800. Sample solution: Dilute the sample solution to 50% solution with dimethyl sulfoxide solution for later use.

[0142] (2) Test steps: Prepare solutions in tubes A, B, and C as follows, incubate at room temperature in the dark, add the same volume of DPPH solution every 3 h, measure the absorbance at 517 nm at different test stages and record the absorbance values, and repeat 3 times for each sample.

[0143] Tube A: 2 mL sample + 2 mL DPPH (0.2 mM) solution;

[0144] Tube B: 2 mL DPPH test solution + 2 mL PBS;

[0145] Tube C: 2mL PBS + 2mL sample;

[0146] Zero adjustment: dimethyl sulfoxide solution.

[0147] (3) Calculation method:

[0148] DPPH free radical scavenging rate: 1-(AC) / B×100%

[0149] Where:

[0150] A—absorbance value of the sample tube, i.e., absorbance value of the solution after the sample reacts with DPPH;

[0151] B—DPPH background absorbance value;

[0152] C—Sample background absorbance

[0153] The results are shown in Table 4.

[0154] Table 4

[0155]

[0156]

[0157] As can be seen from Table 4, the product obtained by the technical solution of the present invention has an inhibitory effect on DPPH. The scavenging rates of DPPH at the 1h test point of Examples 1-15 provided by the present invention are all higher than 80%. However, when any one of Pseudostellariae Cyclotide B, Rhizoma Alpiniae Extract, Herba Lycopodiellae Extract, and Phytol is missing, the scavenging effect of DPPH obtained in Comparative Examples 1-4 is significantly reduced, and when other functionally similar combinations are replaced, the effects obtained in Comparative Examples 5-8 are also far less than the effects obtained by the technical solution of the present invention. Further, it can be seen from Examples 1 and 12-15 that with repeated addition of DPPH solution, the wrinkle-removing and firming composition in Example 1 shows antioxidant fatigue at 3h and 6h, and the scavenging rate of DPPH is not as good as that of the anti-photoaging liposomes formed by encapsulating the wrinkle-removing and firming composition in Examples 12-15, and when the mass ratio of the core layer and the shell layer of the anti-photoaging liposomes is not within the preferred range, the long-term antioxidant capacity of the liposomes will decrease.

[0158] Effect Example 3

[0159] The effect example of the present invention explores the human efficacy of the cream prepared by application examples 1-13, comparative application examples 1-8 and blank application examples, comprising the following steps:

[0160] According to the "Technical Specifications for Safety of Cosmetics" (2015), 176 volunteers aged 45-60 with sensitive skin (lactic acid stinging score>3) were selected and randomly divided into 22 groups, 8 people in each group. The volunteers must have at least obvious fine lines under the eyes or crow's feet, and nasolabial folds are preferred. Under normal circumstances, the volunteers continued to use the product on their entire face for 28 days, and the anti-aging effects of application examples 1-13, comparative application examples 1-8 and blank application examples were evaluated. The specific steps are as follows:

[0161] After cleansing and moisturizing the face every morning and evening, volunteers took an appropriate amount of cream and applied it to the face, massaged it for absorption, and used it on the whole face once in the morning and evening, and returned on days 0, 7, 14, and 28. On the day of the volunteer's visit, the test area washed the face with water without applying any product, and sat quietly in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes. Instruments were used to quantify and photograph the improvement of facial aging indicators, including Cutometer 2mm MPA 580 to measure skin firmness value (R0) and skin plastic elasticity value (R2), which showed significant improvement before and after use, and the sample was judged to have elasticity and firming effect; Visia7 to measure fine lines under the eyes, which showed significant improvement before and after use, and the sample was judged to have firming effect; Primos and Visia 7 to measure crow's feet, which showed significant improvement before and after use, and the sample was judged to have anti-wrinkle effect on crow's feet; Primos to measure nasolabial folds, which showed significant improvement before and after use, and the sample was judged to have anti-wrinkle effect on nasolabial folds; Skin-Colorimeter CL400 and Visia 7 (IPP) to measure skin brightness, which showed significant improvement before and after use, and the sample was judged to have improvement effect on skin brightness; Sebumeter SM 815 to measure skin oil content, which showed significant improvement before and after use, and the sample was judged to have oil control effect;

[0162] Improvement rate before and after use = (X mean before use - X mean after use) / X mean before use × 100%;

[0163] The results are shown in Table 5:

[0164] Table 5

[0165]

[0166]

[0167] Note: When the t-test method is used for statistical analysis, the significance is indicated by * compared with the control group, P-value < 0.05 is indicated by *, and P-value > 0.05 is indicated by ns.

[0168] It can be seen from Table 5 that the product provided by the technical solution of the present invention can significantly improve the firmness, plasticity and elasticity of facial skin, and significantly improve nasolabial folds and crow's feet, and has the effects of significantly improving wrinkle removal and firming, promoting skin elasticity, and having anti-aging effects; at the same time, the product prepared by the technical solution of the present invention can also significantly improve skin color and condition, and reduce oil secretion.

[0169] It can be seen from Application Example 1 and Comparative Application Examples 1-8 that when any one of Pseudostellariae Cyclopeptide B, Tengcha Extract, Herba Lycopodiellae Extract, and Phytol is missing, or when other functionally similar combinations are used to replace them, the effects obtained are far inferior to those obtained by using the technical solution of the present invention; among them, the comparison charts of the crow's feet test results on the left face of volunteers using the cream of Application Example 1 and Comparative Application Example 1 on D0 and D28 are as follows: Figure 1 and Figure 2 As shown, from Figure 1-2 It can also be intuitively seen that the technical solution provided by the present invention can significantly reduce the wrinkle area.

[0170] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A wrinkle-removing and firming composition, characterized in that: The wrinkle-removing and firming composition is composed of the following components in parts by weight: 2-5 parts of Pseudostellaria heterophylla cyclopeptide B, 9-14 parts of rattan tea extract, 3-8 parts of Cynanchum indigoferum extract, and 2-5 parts of phytol; The preparation method of the rattan tea extract comprises the following steps: crushing the rattan tea and adding it to an ethanol aqueous solution, adding citric acid at the same time, then ultrasonic extraction, filtering after the extraction, collecting the filtrate and concentrating it, then adding ethanol to the concentrate for alcohol precipitation, filtering after the precipitation, collecting the solid and drying it to obtain the rattan tea extract; During the preparation of the rattan tea extract, the following conditions are met: a. In ethanol aqueous solution, the mass percentage of ethanol is 70-80%; b. Based on the mass of rattan tea, the amount of citric acid added is 0.2-0.4%, and the amount of ethanol aqueous solution added is 8-12 times; c. During ultrasonic extraction, the ultrasonic power is 100-140W, the time is 20-40min, the temperature is 45-55℃, and the extraction times are 2-3 times; d. In the alcohol precipitation, the mass ratio of the concentrate to ethanol is 1: (0.8-1.2), the alcohol precipitation temperature is 30-40°C, and the alcohol precipitation time is 20-28h; The preparation method of the extract of Herba Lysimachiae comprises the following steps: crushing the extract of Herba Lysimachiae and adding it to an ethanol aqueous solution for leaching, performing ultrasonic extraction after the leaching is completed, filtering after the extraction is completed, collecting the filtrate and concentrating and drying it to obtain the extract of Herba Lysimachiae; During the preparation of the extract of Herba Lysimachiae, the following conditions are met: e. In the ethanol aqueous solution, the mass percentage of ethanol is 75-85%; f. The amount of ethanol aqueous solution added is 8-12 times the mass of the velvet star grass; g. The extraction temperature is 45-55°C and the extraction time is 25-35min; h. During ultrasonic extraction, the ultrasonic power is 250-350W, the time is 50-70min, and the temperature is 55-65℃.

2. A liposome for resisting photoaging, characterized in that: The liposome comprises a core layer and a shell layer, wherein the core layer comprises the wrinkle-removing and firming composition according to claim 1, and the shell layer comprises phospholipids and cholesterol; The mass ratio of the core layer to the shell layer is 1:(0.5-5).

3. The method for preparing the anti-photoaging liposome according to claim 2, characterized in that: The preparation method comprises the following steps: (1) Dissolving phospholipids and cholesterol in an organic solvent, and then evaporating the organic solvent to form a membrane layer; (2) Mixing the phosphate buffer containing the wrinkle-removing and firming composition according to claim 1 with the film layer formed in step (1) and subjecting the mixture to a hydration reaction. After the hydration reaction is completed, ultrasonication and filtration are performed to collect the filter residue to obtain anti-photoaging liposomes.

4. Use of the anti-wrinkle and firming composition according to claim 1 or the anti-photoaging liposome according to claim 2 in the preparation of cosmetics.

5. A facial cream, characterized in that: The facial cream comprises the following components in percentage by mass: 3-10% of the anti-wrinkle and firming composition according to claim 1 or the anti-photoaging liposome according to claim 2, 2-40% of a cosmetic base and the balance water.

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