Alpha-l-fucosidase test kit

Abstract

The application discloses an alpha-L-fucosidase detection kit and relates to the field of kits.The alpha-L-fucosidase detection kit comprises a box body, a box cover is arranged on the top of the box body, a placing cavity is formed in the inside of the box body, a placing seat is fixedly arranged in the inside of the placing cavity, a moving seat is arranged above the placing seat, a plurality of reagent tubes are inserted between the moving seat and the placing seat, and a lifting assembly is arranged at the bottom of the moving seat.When a plurality of reagent tubes need to be used for experiment, the end of the inserting rod is separated from the upper inserting hole by pulling the inserting rod outward, the moving seat can be pulled upward at the moment, a plurality of reagent tubes can be moved upward by the moving strip at the moment, the identification on the plurality of reagent tubes is convenient to check, and the internal reagents in the plurality of reagent tubes can be detected.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of reagent kits, in particular to an alpha-L-fucosidase detection kit. BACKGROUND

[0002] The alpha-L-fucosidase detection kit is an experimental tool for detecting the activity of alpha-L-fucosidase (AFU). Alpha-L-fucosidase is a hydrolase widely existing in human bodies and other organisms and is involved in various physiological processes, including the metabolism of glycoproteins and glycolipids. The alpha-L-fucosidase detection kit is mainly used for measuring the activity of alpha-L-fucosidase in serum or plasma and is usually used for clinical diagnosis and scientific research experiments.

[0003] In the process of preparing the alpha-L-fucosidase detection reagent, different components of the alpha-L-fucosidase detection reagent need to be tested one by one. When testing, a certain volume of substrate solution is added to a 96-well plate or a colorimetric cup, and then mixed rapidly. The reaction system is placed in a 37 DEG C constant temperature incubator to start the reaction. Every fixed time interval, the absorbance value is measured at a specified wavelength by using a spectrophotometer, so that the use effect of the alpha-L-fucosidase detection reagent can be detected. Although the alpha-L-fucosidase detection reagent tubes are usually placed in the inside of the reagent kit, the labels on the outside of the different alpha-L-fucosidase detection reagent tubes are not convenient to observe. Therefore, the application provides an alpha-L-fucosidase detection kit. SUMMARY

[0004] In view of the deficiencies of the prior art, the application provides an alpha-L-fucosidase detection kit, which solves the problem that the alpha-L-fucosidase detection reagent tubes are usually placed in the inside of the reagent kit, and the labels on the outside of the different alpha-L-fucosidase detection reagent tubes are not convenient to observe.

[0005] To achieve the above object, the application is implemented by the following technical scheme: an alpha-L-fucosidase detection kit, comprising a box body, a box cover is installed on the top of the box body, a placing cavity is formed in the inside of the box body, a placing seat is fixedly installed in the inside of the placing cavity, a moving seat is arranged above the placing seat, a plurality of reagent tubes are inserted between the moving seat and the placing seat, and the end portions of the plurality of reagent tubes are in abutment with the upper portion of the moving seat.

[0006] The bottom portion of the moving seat is provided with a lifting assembly, the lifting assembly comprises a moving strip and a plug rod, the top end of the moving strip is fixedly installed below the moving seat, the bottom end of the moving strip is inserted into the inside of the placing seat, the top portion and the bottom portion of the moving strip are both provided with left-right through insertion holes, the plug rod is movably inserted into the side surface of the box body, and the end portion of the plug rod is inserted into one of the insertion holes.

[0007] Preferably, the top of the box body is provided with an extrusion groove, and an extrusion strip is fixedly installed below the box cover. The extrusion strip abuts tightly against the inner wall of the extrusion groove, and fixing blocks are fixedly connected to both sides of the box cover.

[0008] Preferably, a limiting ring is fixedly sleeved on the outer side of the insertion rod, and the limiting ring is slidably connected above the placement seat. A limiting spring is fixedly installed between the side of the limiting ring and the inner wall of the box.

[0009] Preferably, the end of the insertion rod that is externally located is provided with a knob, and a positioning block is fixedly installed on the side of the knob, with the positioning block being movably inserted into the outer wall of the box.

[0010] Preferably, an extension frame is embedded above the movable seat, the extension frame is movably sleeved on the outside of a plurality of reagent tubes located in front, and the ends of the plurality of reagent tubes are in contact with the extension frame. An extension rod is fixedly installed at the bottom of the extension frame, and the bottom end of the extension rod extends downward through the movable seat.

[0011] Preferably, a fixing sleeve is fixedly installed below the movable seat, and a sliding ring is slidably arranged inside the fixing sleeve, with a positioning rod fixedly inserted inside the sliding ring.

[0012] Preferably, one end of the positioning rod is fixedly connected to a pressing block, the pressing block is slidably disposed inside the fixed sleeve, and the end of the pressing block extends outward and closely abuts against the outside of the extension rod, and the other end of the positioning rod extends outward through the fixed sleeve and is fixedly connected to a pull rod.

[0013] Preferably, a compression spring is movably sleeved on the outer side of the positioning rod, and the two ends of the compression spring are respectively fixedly connected to the side of the sliding ring and the inner wall of the fixed sleeve.

[0014] Preferably, the reagent inside the reagent tube consists of trimethylolpropionic acid, 2-chloro-4-nitrobenzene-α-L-fucoside, sodium chloride, and a nonionic surfactant.

[0015] Preferably, the reagent preparation steps inside the reagent tube are as follows:

[0016] Step 1: Dissolve 2-80g of tris(hydroxymethyl)aminoethanesulfonic acid, 0.5-10g of 2-chloro-4-nitrobenzene-α-L-fucoside, 0.1-8g of sodium chloride, and 0.1-5ml of nonionic surfactant in distilled water;

[0017] Step 2: Mix thoroughly and adjust the pH to between 6.5 and 8.5 using a 1 mol / L sodium hydroxide solution;

[0018] Step 3: Adjust the volume to the required level using distilled or double-distilled water.

[0019] This invention discloses an α-L-fucosidase detection kit, which has the following beneficial effects:

[0020] 1. When multiple reagent tubes need to be tested using this α-L-fucosidase assay kit, first pull the insert outwards to disengage the end of the insert from the upper socket. Then, pull the movable seat upwards. Since the ends of multiple reagent tubes are in contact with the upper part of the movable seat, the movable strip will move the multiple reagent tubes upwards until they reach the outside. At this point, insert the end of the insert into the lower socket to fix the position of the movable seat and the multiple reagent tubes. This facilitates checking the markings on the multiple reagent tubes and detecting the reagents inside the multiple reagent tubes.

[0021] 2. In this α-L-fucosidase assay kit, after the movable base and multiple reagent tubes are fixed at a high position, pulling the lever outward causes the positioning rod to slide outward and compress the compression spring. Simultaneously, the compression block at the other end of the positioning rod moves inward toward the fixed sleeve, thus separating the compression block from the outer side of the extension rod. At this point, the extension rod can be pushed upward, thereby moving the extension frame upward, and the ends of multiple reagent tubes are in contact with the extension frame, allowing the multiple reagent tubes at the front to move upward as well. Then, releasing the lever causes the end of the compression block to extend outward again under the action of the compression spring, tightly abutting against the outer side of the extension rod, thereby fixing the positions of the extension rod, extension frame, and multiple reagent tubes at the front. This results in the multiple reagent tubes at the front and back being placed in a stepped manner, making observation more convenient.

[0022] 3. When the α-L-fucosidase assay kit requires pulling the insert outward, first pull the knob outward. This moves the insert outward, causing the limiting ring to move and compressing the limiting spring. Simultaneously, it causes the positioning block to slide outward from the outer wall of the kit until it is fully moved to the outside. Rotating the knob and positioning block prevents the positioning block from resetting, thus fixing the position of the insert. This facilitates pulling the moving seat upward until the moving seat and moving strip are pulled to their highest position. Rotating the knob and positioning block again resets the positioning block. Under the action of the limiting spring, the end of the insert is inserted into the hole below the moving strip, thus fixing the position of the moving seat and multiple reagent tubes. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the structure of the present invention;

[0025] Figure 2 This is a schematic diagram of the structure of the box lid of the present invention;

[0026] Figure 3 This is a schematic diagram of the structure of the box and reagent tube of the present invention;

[0027] Figure 4 This is a cross-sectional view of the box body of the present invention;

[0028] Figure 5 This is a schematic diagram of the internal structure of the box body of the present invention;

[0029] Figure 6 This is a schematic diagram of the structure of the insertion rod of the present invention;

[0030] Figure 7 This is a schematic diagram of the structure of the movable base of the present invention;

[0031] Figure 8 This is a schematic diagram of the extension frame of the present invention;

[0032] Figure 9 This is a schematic diagram of the structure of the fixed sleeve, positioning rod and extrusion block of the present invention.

[0033] In the diagram: 1. Box body; 101. Placement cavity; 102. Placement seat; 103. Movable seat; 104. Reagent tube; 105. Squeezing groove; 2. Box lid; 201. Squeezing strip; 202. Fixing block; 3. Lifting assembly; 301. Movable strip; 302. Insertion rod; 303. Insertion hole; 304. Limiting ring; 305. Limiting spring; 306. Knob; 307. Positioning block; 4. Extension frame; 401. Extension rod; 402. Fixing sleeve; 403. Sliding ring; 404. Positioning rod; 405. Squeezing block; 406. Pull rod; 407. Squeezing spring. Detailed Implementation

[0034] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are described clearly and completely. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] This application provides an α-L-fucosidase detection kit, which solves the problem that the α-L-fucosidase detection reagent tubes 104 are generally placed inside the kit, making it inconvenient to observe the labels pasted on the outside of different α-L-fucosidase detection reagent tubes 104. When multiple reagent tubes 104 need to be tested, first pull the insert rod 302 outward so that the end of the insert rod 302 disengages from the upper insertion hole 303. Then, the movable seat 103 can be pulled upward. Since the ends of multiple reagent tubes 104 are all in contact with the upper part of the movable seat 103, the movable bar 301 can move the multiple reagent tubes 104 upward until they are moved to the outside. At this time, the end of the insert rod 302 is inserted into the lower insertion hole 303 to fix the position of the movable seat 103 and the multiple reagent tubes 104. This makes it convenient to check the labels on the multiple reagent tubes 104 and to detect the reagents inside the multiple reagent tubes 104.

[0036] To better understand the above technical solutions, the following will provide a detailed explanation of the technical solutions in conjunction with the accompanying drawings and specific implementation methods.

[0037] This invention discloses an α-L-fucosidase detection kit, according to the attached... Figures 1-9 As shown, the device includes a box body 1, a box cover 2 installed on the top of the box body 1, a placement cavity 101 opened inside the box body 1, a placement seat 102 fixedly installed inside the placement cavity 101, a movable seat 103 arranged above the placement seat 102, and a plurality of reagent tubes 104 inserted between the movable seat 103 and the placement seat 102, with the ends of the plurality of reagent tubes 104 all abutting against the top of the movable seat 103.

[0038] The bottom of the movable base 103 is provided with a lifting component 3. The lifting component 3 includes a moving bar 301 and a plug rod 302. The top end of the moving bar 301 is fixedly installed below the movable base 103, and the bottom end of the moving bar 301 is inserted into the interior of the placement base 102. The top and bottom of the moving bar 301 are provided with through holes 303. The plug rod 302 is movably inserted into the side of the box body 1, and the end of the plug rod 302 is inserted into one of the holes 303.

[0039] During use, when multiple reagent tubes 104 need to be tested, first pull the insert rod 302 outward so that the end of the insert rod 302 disengages from the upper insertion hole 303. Then, the movable seat 103 can be pulled upward. Since the ends of multiple reagent tubes 104 are all in contact with the upper part of the movable seat 103, the movable bar 301 can drive the multiple reagent tubes 104 to move upward until the multiple reagent tubes 104 are moved to the outside. At this time, insert the end of the insert rod 302 into the lower insertion hole 303 to fix the position of the movable seat 103 and the multiple reagent tubes 104. This makes it convenient to check the markings on the multiple reagent tubes 104 and to test the reagents inside the multiple reagent tubes 104.

[0040] Furthermore, the top of the box body 1 is provided with an extrusion groove 105, and an extrusion strip 201 is fixedly installed below the box cover 2. The extrusion strip 201 is tightly abutted against the inner wall of the extrusion groove 105, and fixing blocks 202 are fixedly connected to both sides of the box cover 2.

[0041] By using the compression strip 201 and compression groove 105, when the compression strip 201 and the inner wall of the compression groove 105 are tightly connected, the lid 2 can be fixedly placed on the top of the box body 1, thereby effectively protecting the multiple reagent tubes 104 inside.

[0042] Specifically disclosed, a limiting ring 304 is fixedly sleeved on the outer side of the insertion rod 302, and the limiting ring 304 is slidably connected above the placement seat 102. A limiting spring 305 is fixedly installed between the side of the limiting ring 304 and the inner wall of the box 1.

[0043] Furthermore, a knob 306 is rotatably provided at the external end of the insertion rod 302, and a positioning block 307 is fixedly installed on the side of the knob 306, and the positioning block 307 is movably inserted into the outer wall of the box 1.

[0044] When it is necessary to pull the insertion rod 302 outward, first pull the knob 306 outward. On the one hand, this causes the insertion rod 302 to move outward, which in turn moves the limiting ring 304 and compresses the limiting spring 305. On the other hand, it causes the positioning block 307 to slide outward from the outer wall of the box 1 until the positioning block 307 is completely moved to the outside. At this time, rotating the knob 306 and the positioning block 307 prevents the positioning block 307 from resetting, thus fixing the position of the insertion rod 302. This makes it easier to pull the moving seat 103 upward until the moving seat 103 and the moving bar 301 are pulled to the highest position. Then, rotating the knob 306 and the positioning block 307 again allows the positioning block 307 to reset. Under the action of the limiting spring 305, the end of the insertion rod 302 is inserted into the insertion hole 303 below the moving bar 301, thus fixing the position of the moving seat 103 and the multiple reagent tubes 104.

[0045] Specifically disclosed, an extension frame 4 is embedded above the movable base 103. The extension frame 4 is movably sleeved on the outside of a plurality of reagent tubes 104 located in front, and the ends of the plurality of reagent tubes 104 are in contact with the extension frame 4. An extension rod 401 is fixedly installed at the bottom of the extension frame 4, and the bottom end of the extension rod 401 extends downward through the movable base 103.

[0046] Specifically disclosed, a fixed sleeve 402 is fixedly installed below the movable seat 103, and a sliding ring 403 is slidably arranged inside the fixed sleeve 402. A positioning rod 404 is fixedly inserted into the sliding ring 403.

[0047] Furthermore, one end of the positioning rod 404 is fixedly connected to a pressing block 405, the pressing block 405 is slidably disposed inside the fixed sleeve 402, and the end of the pressing block 405 extends outward and closely abuts against the outside of the extension rod 401. The other end of the positioning rod 404 extends outward through the fixed sleeve 402 and is fixedly connected to a pull rod 406.

[0048] Furthermore, a compression spring 407 is movably sleeved on the outer side of the positioning rod 404, and the two ends of the compression spring 407 are respectively fixedly connected to the side of the sliding ring 403 and the inner wall of the fixed sleeve 402.

[0049] After the movable seat 103 and the multiple reagent tubes 104 are fixed at a high position, pull the lever 406 outward to make the positioning lever 404 slide outward and compress the compression spring 407. At the same time, the compression block 405 at the other end of the positioning lever 404 moves towards the inside of the fixed sleeve 402, thereby separating the compression block 405 from the outside of the extension lever 401. At this time, the extension lever 401 can be pushed upward, thereby driving the extension frame 4 to move upward, and the ends of the multiple reagent tubes 104 are in contact with the extension frame 4, so that the multiple reagent tubes 104 in front can move upward. Then, release the lever 406. Under the action of the compression spring 407, the end of the compression block 405 extends outward again and tightly abuts against the outside of the extension lever 401, thereby fixing the position of the extension lever 401, the extension frame 4 and the multiple reagent tubes 104 in front, so that the multiple reagent tubes 104 in front and behind are placed in a stepped shape, which makes it easier to observe.

[0050] It is particularly important to emphasize that the reagents inside reagent tube 104 consist of trimethylolpropionic acid (MES), 2-chloro-4-nitrobenzene-α-L-fucoside, sodium chloride, and a nonionic surfactant.

[0051] It is important to emphasize that the reagent preparation steps inside reagent tube 104 are as follows:

[0052] Step 1: Dissolve 2-80g of trimethylolpropionic acid (MES), 0.5-10g of 2-chloro-4-nitrobenzene-α-L-fucoside, 0.1-8g of sodium chloride, and 0.1-5ml of nonionic surfactant in distilled water;

[0053] Step 2: Mix thoroughly and adjust the pH to between 6.5 and 8.5 using a 1 mol / L sodium hydroxide solution;

[0054] Step 3: Adjust the volume to the required level using distilled or double-distilled water.

[0055] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of this invention is defined by the appended claims and their equivalents.

Claims

1. An α-L-fucosidase detection kit, comprising a housing (1), wherein a lid (2) is mounted on the top of the housing (1), characterized in that, The box body (1) has a placement cavity (101) inside, and a placement seat (102) is fixedly installed inside the placement cavity (101). A movable seat (103) is provided above the placement seat (102), and multiple reagent tubes (104) are inserted between the movable seat (103) and the placement seat (102). The ends of the multiple reagent tubes (104) are all in contact with the top of the movable seat (103). The bottom of the movable seat (103) is provided with a lifting component (3). The lifting component (3) includes a moving bar (301) and a plug rod (302). The top end of the moving bar (301) is fixedly installed below the movable seat (103), and the bottom end of the moving bar (301) is inserted into the interior of the placement seat (102). The top and bottom of the moving bar (301) are provided with through holes (303) that pass through from left to right. The plug rod (302) is movably inserted into the side of the box (1), and the end of the plug rod (302) is inserted into one of the holes (303). An extension frame (4) is embedded above the movable seat (103). The extension frame (4) is movably sleeved on the outside of a plurality of reagent tubes (104) located in front, and the ends of the plurality of reagent tubes (104) are in contact with the extension frame (4). An extension rod (401) is fixedly installed at the bottom of the extension frame (4), and the bottom end of the extension rod (401) extends downward through the movable seat (103). A fixed sleeve (402) is fixedly installed below the movable seat (103). A sliding ring (403) is slidably arranged inside the fixed sleeve (402), and a positioning rod (404) is fixedly inserted inside the sliding ring (403). One end of the positioning rod (404) is fixedly connected to a pressing block (405), the pressing block (405) is slidably disposed inside the fixed sleeve (402), and the end of the pressing block (405) extends outward and closely abuts against the outside of the extension rod (401). The other end of the positioning rod (404) extends outward through the fixed sleeve (402) and is fixedly connected to a pull rod (406). A compression spring (407) is movably sleeved on the outer side of the positioning rod (404), and the two ends of the compression spring (407) are respectively fixedly connected to the side of the sliding ring (403) and the inner wall of the fixed sleeve (402).

2. The α-L-fucosidase detection kit according to claim 1, characterized in that, The top of the box body (1) is provided with an extrusion groove (105), and an extrusion strip (201) is fixedly installed below the box cover (2). The extrusion strip (201) is tightly abutted against the inner wall of the extrusion groove (105), and a fixing block (202) is fixedly connected to both sides of the box cover (2).

3. The α-L-fucosidase detection kit according to claim 1, characterized in that, A limiting ring (304) is fixedly sleeved on the outside of the insertion rod (302), and the limiting ring (304) is slidably connected above the placement seat (102). A limiting spring (305) is fixedly installed between the side of the limiting ring (304) and the inner wall of the box (1).

4. The α-L-fucosidase detection kit according to claim 1, characterized in that, The insertion rod (302) has a knob (306) rotatably mounted on its external end, and a positioning block (307) is fixedly installed on the side of the knob (306). The positioning block (307) is movably inserted into the outer wall of the box (1).

5. The α-L-fucosidase detection kit according to claim 1, characterized in that, The reagent inside the reagent tube (104) consists of trimethylolpropionic acid (MES), 2-chloro-4-nitrobenzene-α-L-fucoside, sodium chloride and a nonionic surfactant.

6. The α-L-fucosidase detection kit according to claim 5, characterized in that, The reagent preparation steps inside the reagent tube (104) are as follows: Step 1: Dissolve 2-80g of trimethylolpropionic acid (MES), 0.5-10g of 2-chloro-4-nitrobenzene-α-L-fucoside, 0.1-8g of sodium chloride, and 0.1-5ml of nonionic surfactant in distilled water; Step 2: Mix thoroughly and adjust the pH to between 6.5 and 8.5 using a 1 mol / L sodium hydroxide solution; Step 3: Adjust the volume to the required level using distilled or double-distilled water.

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