Tumor cell culture device

Abstract

The present invention belongs to the field of cell culture technology, and provides a tumor cell culture device, including a box, wherein the box is evenly divided into a number of culture chambers by a number of partitions; a number of lifting platform mechanisms, wherein the number of lifting platform mechanisms are respectively arranged in the number of culture chambers, and the lifting platform mechanism includes a lifting platform and a lifting drive assembly, and an isolation plate is arranged on the side wall of the culture chamber, and a first through hole is opened on the isolation plate, and the lifting platform fits with the first through hole after being raised; a liquid exchange assembly, wherein the liquid exchange assembly includes a column rotatably connected to the center of the box, a rotating drive member is arranged between the column and the box, a liquid exchange arm mechanism is arranged on the column, and the liquid exchange arm mechanism is used to replace the culture fluid in the culture dish, and a number of partitions are provided with isolation door members for the liquid exchange arm mechanism to pass through. The present invention can carry out tumor cell culture under different conditions in one box, and at the same time, liquid exchange and taking out the culture dish have little impact on the culture environment.

Description

Technical Field

[0001] The invention belongs to the technical field of cell culture, and in particular relates to a tumor cell culture device. Background Art

[0002] Tumor cells occupy a core position in tissue culture and are an extremely important means of studying carcinogenesis, anticancer drug detection, and cancer molecular biology, playing an immeasurable role in clarifying and solving cancer. In related technologies, a special culture device is usually used to culture tumor cells. The culture medium and tumor cells are placed in the culture dish for static culture, and the culture efficiency of tumor cells is ensured by adjusting the internal temperature of the device.

[0003] However, the existing culture devices still have the following problems: 1. The culture dishes are all placed in the same culture space with the same culture environment. When some of the culture dishes need to be observed or the fluid needs to be changed, the door needs to be opened, which will cause the inside of the culture device to come into contact with the external environment, affecting the stability of its internal environment and also affecting other culture dishes; 2. When changing the fluid, the culture dishes need to be taken out, causing the tumor cells to come into contact with the external environment, which is not conducive to the cultivation of tumor cells; 3. Since the culture device can only provide one culture environment, horizontal comparative culture operations cannot be performed. Summary of the invention

[0004] The purpose of the present invention is to provide a tumor cell culture device to solve the above problems, so as to achieve the purpose of being able to culture tumor cells under different conditions in one box, while having little impact on the culture environment by changing the liquid and taking out the culture dish.

[0005] To achieve the above object, the present invention provides the following solution: a tumor cell culture device, comprising:

[0006] A box body, wherein the box body is evenly divided into a plurality of culture chambers by a plurality of partitions, a plurality of box doors are arranged on the top of the box body, and the plurality of box doors are respectively arranged corresponding to the plurality of culture chambers;

[0007] A plurality of lifting platform mechanisms, wherein the plurality of lifting platform mechanisms are respectively arranged in the plurality of culture chambers, the lifting platform mechanisms include a lifting platform and a lifting drive assembly, the lifting platform is used to place a culture dish, an isolation plate is arranged on the side wall of the culture chamber, a first through hole is opened on the isolation plate, the lifting platform is fitted with the first through hole after being raised, and the box door is arranged above the isolation plate;

[0008] A fluid exchange component, the fluid exchange component includes a column rotatably connected to the center of the box, a rotating drive member is arranged between the column and the box, a fluid exchange arm mechanism is arranged on the column, the fluid exchange arm mechanism is used to replace the culture fluid in the culture dish, and several of the partitions are provided with isolation door members for the passage of the fluid exchange arm mechanism.

[0009] Preferably, a horizontal partition plate is fixedly connected to the inner side of the box body, the lifting platform and the partition plate are arranged above the horizontal partition plate, the rotating drive member is arranged below the horizontal partition plate, and the bottom of the column passes through the horizontal partition plate and is transmission-connected to the rotating drive member.

[0010] Preferably, the lifting drive assembly includes an electric telescopic rod vertically fixedly connected to the top of the horizontal partition plate, and the telescopic end of the electric telescopic rod is fixedly connected to the top of the lifting platform.

[0011] Preferably, a plurality of guide columns are slidably penetrated through the edge of the lifting platform, and the plurality of guide columns are vertically fixedly connected to the horizontal partition plate.

[0012] Preferably, the rotating drive member includes a turntable horizontally rotatably connected to the bottom of the box and a second motor fixedly connected to the bottom of the box, a gear ring is fixedly sleeved on the side wall of the turntable, a gear is fixedly sleeved on the output shaft of the second motor, the gear is meshed with the gear ring, the column is vertically fixedly connected to the top of the turntable, and the column and the turntable are coaxially arranged.

[0013] Preferably, the fluid exchange arm mechanism includes a fluid exchange arm horizontally fixedly connected to the top of the side wall of the column, a groove is provided at the bottom of the fluid exchange arm, one end of the fluid exchange arm is hingedly connected to the side of the groove away from the column, the groove is used to accommodate the fluid exchange arm, the hinge shaft of the fluid exchange arm is transmission-connected to the first motor, and the first motor is fixedly connected to the fluid exchange arm.

[0014] Preferably, a two-way liquid pump is fixedly connected to the turntable, one connecting port of the two-way liquid pump is connected to one end of a first hose, the other end of the first hose is arranged corresponding to the culture dish through the column, the liquid exchange arm and the liquid exchange support arm, the other connecting port of the two-way liquid pump is connected to one end of a second hose, and the other end of the second hose extends out of the box.

[0015] Preferably, the isolation door member includes a through hole opened on the partition, the through hole is used to ensure the passage of the fluid exchange arm, and the top of the through hole is hinged with an opening and closing partition, and the opening and closing partition closes the through hole when not pushed by the fluid exchange arm.

[0016] Preferably, a second through hole is opened in the center of the lifting platform, and a deflection platform is rotatably connected in the second through hole. The deflection platform is used to place the culture dish, and two groups of elastic bands are fixedly connected to the bottom of the lifting platform. The two groups of elastic bands are respectively fitted with the bottom of the deflection platform, and the two groups of elastic bands are located on both sides of the rotation axis of the deflection platform.

[0017] Preferably, an iron rod is fixedly connected to the bottom of the deflection table, a cylinder is fixedly connected to the turntable, an electromagnet is fixedly connected to the telescopic end of the cylinder, the electromagnet is arranged corresponding to the iron rod, and the telescopic direction of the cylinder is arranged perpendicular to the rotation axis direction of the deflection table.

[0018] Compared with the prior art, the present invention has the following advantages and technical effects: the main function of the partition is to divide the box body into several independent culture chambers, so that the temperature, humidity and other conditions in different culture chambers do not affect each other; the main function of the lifting platform is to drive the culture dish to be raised or lowered. When it is necessary to change the liquid or take and place the culture dish, the lifting platform is lifted, and during the culture process, the lifting platform is lowered to an appropriate height; the main function of the isolation plate is to isolate the space on the upper and lower sides of the lifting platform through the sealing cooperation between the lifting platform and the isolation plate after the lifting platform is lifted, so that the temperature, humidity and other conditions under the lifting platform are maintained stable, and when the box door is opened, the impact on the culture environment is reduced. After the box door is closed, the culture dish can be quickly restored to a suitable culture environment as the lifting platform descends; the main function of the liquid exchange arm mechanism is to extend the pipeline required for liquid exchange into the culture dish; the main function of the rotating drive member is to drive the column to rotate so that the liquid exchange arm mechanism can be moved to the culture dish that needs liquid exchange. On the whole, the present invention can carry out tumor cell culture under different conditions in one box, improve the utilization efficiency, and can provide experimental personnel with comparative culture. At the same time, the culture device of the present invention can complete the liquid replacement work without the culture dish contacting the external environment. In the process of taking and placing the culture dish, the original culture environment can be maintained to the greatest extent without being destroyed, reducing the impact of the external environment on the culture process. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 is a schematic diagram of the culture device of the present invention;

[0021] Figure 2 It is a schematic diagram of the lifting platform mechanism and the liquid changing assembly of the present invention;

[0022] Figure 3 is a schematic diagram of a lifting drive assembly of the present invention;

[0023] Figure 4 A schematic diagram of the interaction between the electromagnet and the iron rod of the present invention;

[0024] Figure 5 It is a schematic diagram of the positions of the column, the rotating drive member and the box body of the present invention;

[0025] Figure 6 It is a schematic diagram of the connection between the column and the rotating driving member of the present invention;

[0026] Figure 7 It is a schematic diagram of the deflection table and the lifting table of the present invention;

[0027] Figure 8 It is a schematic diagram of the position of the bottom hole of the present invention;

[0028] Among them, 1. box body; 2. supporting legs; 3. elastic belt; 4. box door; 5. isolation board; 6. lifting platform; 7. deflection platform; 8. culture dish; 9. guide column; 10. partition; 11. opening and closing partition; 12. liquid exchange arm; 13. liquid exchange support arm; 14. first motor; 15. electric telescopic rod; 16. horizontal partition; 17. turntable; 18. column; 19. cylinder; 20. electromagnet; 21. iron rod; 22. two-way liquid pump; 23. second motor; 24. gear; 25. gear ring; 26. through hole; 27. bottom hole. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0031] Reference Figure 1-Figure 8 The present invention provides a tumor cell culture device, comprising:

[0032] A box body 1, wherein the box body 1 is evenly divided into a plurality of culture chambers by a plurality of partitions 10, and a plurality of box doors 4 are arranged on the top of the box body 1, and the plurality of box doors 4 are respectively arranged corresponding to the plurality of culture chambers;

[0033] A plurality of lifting platform mechanisms, the plurality of lifting platform mechanisms are respectively arranged in a plurality of culture chambers, the lifting platform mechanisms include a lifting platform 6 and a lifting drive assembly, the lifting platform 6 is used to place a culture dish 8, an isolation plate 5 is arranged on the side wall of the culture chamber, a first through hole is opened on the isolation plate 5, the lifting platform 6 is raised to fit with the first through hole, and the box door 4 is arranged above the isolation plate 5;

[0034] The fluid exchange component includes a column 18 rotatably connected to the center of the box body 1, a rotating drive member is arranged between the column 18 and the box body 1, a fluid exchange arm mechanism is arranged on the column 18, the fluid exchange arm mechanism is used to replace the culture fluid in the culture dish 8, and a plurality of partitions 10 are provided with isolation door members for the passage of the fluid exchange arm mechanism.

[0035] The main function of the partition 10 is to divide the box body 1 into several independent culture chambers so that the temperature, humidity and other conditions in different culture chambers do not affect each other; the main function of the box door 4 is to facilitate the operator to take and place the culture dishes in the corresponding culture chamber; the main function of the lifting platform 6 is to drive the culture dish to be raised or lowered. When it is necessary to change the liquid or take and place the culture dish, the lifting platform 6 is lifted. During the culture process, the lifting platform 6 is lowered to a suitable height; the main function of the isolation plate 5 is to isolate the space on the upper and lower sides of the lifting platform 6 through the sealing cooperation between the lifting platform 6 and the isolation plate 5 after the lifting platform 6 is lifted, so that the temperature, humidity and other conditions under the lifting platform 6 are maintained stable, and when the box door 4 is opened, the impact on the culture environment is reduced. After the box door 4 is closed, the culture dish 8 can be quickly restored to a suitable culture environment as the lifting platform 6 descends; the main function of the liquid exchange arm mechanism is to extend the pipeline required for liquid exchange into the culture dish 8; the main function of the rotating drive member is to drive the column 18 to rotate so that the liquid exchange arm mechanism can be moved to the culture dish that needs liquid exchange. On the whole, the present invention can carry out tumor cell culture under different conditions in one box, improve the utilization efficiency, and can provide experimental personnel with comparative culture. At the same time, the culture device of the present invention can complete the liquid replacement work without the culture dish contacting the external environment. In the process of taking and placing the culture dish, the original culture environment can be maintained to the greatest extent without being destroyed, reducing the impact of the external environment on the culture process.

[0036] In a further optimized solution, each culture chamber is provided with a temperature control device, a humidity control device, an illumination device, etc., for adjusting the culture environment in the culture chamber. The temperature control device, the humidity control device, the illumination device, etc. are arranged below the isolation plate 5. Since the temperature control device, the humidity control device, and the illumination device are conventionally arranged, their structures and working principles are not described in detail.

[0037] To further optimize the solution, a control unit is also provided, and the control unit is electrically connected to the lifting drive assembly, the liquid exchange arm mechanism and the rotating drive member.

[0038] To further optimize the solution, the box door 4 can be made of transparent material.

[0039] According to a further optimization scheme, a horizontal partition plate 16 is fixedly connected to the inner side of the box body 1, the lifting platform 6 and the partition plate 10 are arranged above the horizontal partition plate 16, the rotating drive member is arranged below the horizontal partition plate 16, and the bottom of the column 18 passes through the horizontal partition plate 16 and is connected to the rotating drive member.

[0040] According to a further optimized solution, the lifting drive assembly includes an electric telescopic rod 15 vertically fixedly connected to the top of the horizontal partition plate 16 , and the telescopic end of the electric telescopic rod 15 is fixedly connected to the top of the lifting platform 6 .

[0041] According to a further optimized solution, a plurality of guide posts 9 are slidably penetrated through the edge of the lifting platform 6 , and the plurality of guide posts 9 are vertically fixedly connected to the horizontal partition plate 16 .

[0042] like Figure 2 and Figure 3 As shown, the main function of the plurality of guide posts 9 is to maintain the lifting platform 6 in a horizontal state during the lifting process. The electric telescopic rod 15 performs a telescopic action under the control of the control unit to achieve the lifting and lowering of the lifting platform 6.

[0043] like Figure 2 As shown, the box body 1 is divided into four culture chambers by four groups of partitions 10. In the culture chamber on the left side, the lifting platform 6 is lifted to a set height. At this time, the lifting platform 6 is in contact with the isolation board 5. Since the temperature control equipment, humidity control equipment and lighting equipment are arranged below the isolation board 5, the original culture environment will be maintained in the space below the lifting platform 6. At this time, opening the corresponding box door 4 will only affect the environmental conditions in the part of the space above the lifting platform 6. When the box door 4 is closed, as the lifting platform 6 descends, the culture dish 8 will enter the stable environment below. Through the buffering effect of the stable environment below, the tumor cells in the culture dish 8 will quickly enter the appropriate culture environment.

[0044] A further optimized solution is that the rotating drive member includes a turntable 17 horizontally rotatably connected to the bottom of the box body 1 and a second motor 23 fixedly connected to the bottom of the box body 1, a gear ring 25 is fixedly sleeved on the side wall of the turntable 17, a gear 24 is fixedly sleeved on the output shaft of the second motor 23, the gear 24 is meshed with the gear ring 25, and a column 18 is vertically fixedly connected to the top of the turntable 17, and the column 18 and the turntable 17 are coaxially arranged.

[0045] like Figure 5 and Figure 6As shown, the control unit controls the rotation angle of the second motor 23, and the second motor 23 rotates through the gear 24 to drive the ring gear 25 to rotate, thereby driving the turntable 17 to rotate, and the rotation of the turntable 17 drives the column 18, so that the liquid exchange arm mechanism on the column 18 rotates to the culture chamber that needs to change the liquid.

[0046] A further optimized solution is that the fluid exchange arm mechanism includes a fluid exchange arm 12 horizontally fixedly connected to the top of the side wall of the column 18, and a groove is provided at the bottom of the fluid exchange arm 12. One end of a fluid exchange arm 13 is hingedly connected to the side of the groove away from the column 18, and the groove is used to accommodate the fluid exchange arm 13. The hinge shaft of the fluid exchange arm 13 is transmission-connected to the first motor 14, and the first motor 14 is fixedly connected to the fluid exchange arm 12.

[0047] like Figure 2 and Figure 6 As shown, when the liquid exchange arm 12 is rotated into place, the control unit drives the liquid exchange support arm 13 to rotate a certain angle by controlling the rotation angle of the first motor 14, so that the liquid exchange support arm 13 is rotated out of the groove, so that the bottom of the liquid exchange support arm 13 is in the culture dish 8 where the liquid is to be changed.

[0048] A further optimized solution is that a two-way liquid pump 22 is fixedly connected to the turntable 17, one connection port of the two-way liquid pump 22 is connected to one end of a first hose, the other end of the first hose is arranged corresponding to the culture dish 8 through the column 18, the liquid exchange arm 12 and the liquid exchange support arm 13, and the other connection port of the two-way liquid pump 22 is connected to one end of a second hose, and the other end of the second hose extends out of the box 1.

[0049] like Figure 5 As shown, when it is necessary to extract the old culture fluid in the culture dish 8, the control unit controls the liquid exchange arm 13 to extend the first hose into the culture dish 8, starts the two-way liquid pump 22, extracts the old culture fluid through the first hose and discharges it from the box 1 through the second hose. When it is necessary to add new culture fluid to the culture dish 8, the control unit controls the two-way liquid pump 22 to reverse, and pumps the new culture fluid into the culture dish 8 through the second hose and the first hose in turn.

[0050] A further optimized solution is that the isolation door member includes a perforation opened on the partition 10, the perforation is used to ensure that the fluid exchange arm 12 passes through, and the top of the perforation is hinged with an opening and closing partition 11, which closes the perforation when not pushed by the fluid exchange arm 12.

[0051] A further optimized solution is that the interior of the column 18 is a hollow structure, the center of the turntable 17 is provided with an opening, and the bottom hole 27 is provided at the bottom of the box body 1. The end of the second rubber hose away from the two-way liquid pump 22 passes through the column 18, the turntable 17 and the bottom hole 27 to pass out of the box body 1, and the first rubber hose passes through the hollow structure in the column 18 and is fixedly connected to the liquid exchange arm 13.

[0052] like Figure 2 and Figure 5 As shown, the rotating shaft on the opening and closing partition 11 is set at the top thereof. When no external force is applied, the opening and closing partition 11 is in a vertical state due to gravity, overlaps with the partition 10, closes the perforation, and ensures that the environments of the culture chambers do not affect each other. When the column 18 drives the liquid exchange arm 12 to rotate, the liquid exchange arm 12 pushes the opening and closing partition 11 through the perforation on the partition 10, so that the liquid exchange arm 12 can pass through several partitions 10 at will.

[0053] A further optimized solution is that a second through hole is opened in the center of the lifting platform 6, and a deflection table 7 is rotatably connected in the second through hole. The deflection table 7 is used to place a culture dish 8, and two groups of elastic bands 3 are fixedly connected to the bottom of the lifting platform 6. The two groups of elastic bands 3 are respectively fitted with the bottom of the deflection table 7, and the two groups of elastic bands 3 are located on both sides of the rotation axis of the deflection table 7.

[0054] like Figure 7 As shown, the main function of the two groups of elastic bands 3 is to support the deflection table 7 and ensure that the deflection table 7 will not deflect when no external force is applied.

[0055] A further optimized solution is that an iron rod 21 is fixedly connected to the bottom of the deflection table 7, a cylinder 19 is fixedly connected to the turntable 17, an electromagnet 20 is fixedly connected to the telescopic end of the cylinder 19, the electromagnet 20 is arranged corresponding to the iron rod 21, and the telescopic direction of the cylinder 19 is arranged perpendicular to the rotation axis direction of the deflection table 7.

[0056] To further optimize the solution, a through hole 26 is provided at the bottom of the column 18, and the air pipe on the cylinder 19 is connected to the external air supply device through the through hole 26 and the bottom hole 27. The control unit controls the air pressure provided by the external air supply device and controls the circumferential direction of the solenoid valve, so that the telescopic end of the cylinder 19 can perform reciprocating telescopic motion, and the telescopic process is gentle.

[0057] like Figure 4 and Figure 7 As shown, after the culture dish is replaced, the lifting platform 6 is driven down by the electric telescopic rod 15. Then, the turntable 17 is rotated by the second motor 23, so that the electromagnet 20 on the cylinder 19 is rotated to the bottom of the iron rod 21, and the electromagnet is energized by the control unit. Then, the cylinder 19 is controlled by the solenoid valve to make a gentle reciprocating telescopic movement, driving the electromagnet 20 to make a reciprocating motion, and then through magnetic induction, the iron rod 21 is driven to make the deflection table 7 reciprocate around the rotation axis, and the culture dish 8 is shaken, so that the culture fluid is in good contact with the tumor cells in the culture dish.

[0058] The working process of this embodiment is as follows: the control unit controls the electric telescopic rod 15 to raise the lifting platform 6, so that the operator can place the culture dish 8 on the deflection platform 7 through the box door 4. Afterwards, as the electric telescopic rod 15 retracts, the lifting platform 6 descends and tumor cell culture begins.

[0059] When a culture dish needs to be replaced, the control unit can control the second motor 23 to rotate and move the angle, rotate the liquid replacement arm 12 to the corresponding culture chamber, and then control the first motor 14 to open the liquid replacement arm 13 to a vertical state, and then control the electric telescopic rod 15 to lift the lifting platform 6 until the first rubber hose on the liquid replacement arm 13 enters the culture liquid in the culture dish 8, start the two-way liquid pump 22, extract the old culture liquid from the culture dish 8, and discharge it through the second rubber hose. After that, extend the second rubber hose into the new culture liquid, control the two-way liquid pump 22 to reverse, and pump the new culture liquid into the culture dish 8 through the first rubber hose to complete the liquid replacement. The control unit controls the first motor 14 to reset the fluid exchange arm 13, controls the electric telescopic rod 15 to retract, and lowers the lifting platform 6 to the initial position. The second motor 23 is then controlled to drive the turntable 17 to rotate, so that the electromagnet 20 corresponds to the iron rod 21 under the deflection table 7. The control unit energizes the electromagnet 20 and controls the cylinder 19 to reciprocate and retract, thereby shaking the culture dish 8 to ensure uniform contact between the culture fluid and the tumor cells.

[0060] When a culture dish 8 needs to be taken out to observe the tumor cells therein, the electric telescopic rod 15 is controlled to lift the lifting platform 6 to fit with the isolation plate 5, and then the corresponding box door 4 is opened to take out the culture dish 8. After the observation is completed, the culture dish 8 is reset, the box door 4 is closed, and the lifting platform 6 is lowered to the initial position.

[0061] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0062] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A tumor cell culture device, characterized in that: include: A box body (1), wherein the box body (1) is divided into a plurality of independent culture chambers by a plurality of partitions (10), and a plurality of box doors (4) are arranged on the top of the box body (1), and the plurality of box doors (4) are respectively arranged corresponding to the plurality of culture chambers; A plurality of lifting platform mechanisms, wherein the plurality of lifting platform mechanisms are respectively arranged in the plurality of culture chambers, the lifting platform mechanisms comprising a lifting platform (6) and a lifting drive assembly, the lifting platform (6) being used to place a culture dish (8), isolation plates (5) being respectively arranged on the side walls of the plurality of culture chambers, the isolation plates (5) being provided with a first through hole, the lifting platform (6) being fitted with the first through hole after being raised so as to isolate the space on the upper and lower sides of the lifting platform (6), and tumor cells being cultured after the lifting platform (6) is lowered, and the box door (4) is arranged above the isolation plate (5); a fluid exchange assembly, the fluid exchange assembly comprising a column (18) rotatably connected to the center of the box (1), a rotation driving member being provided between the column (18) and the box (1), a fluid exchange arm mechanism being provided on the column (18), the fluid exchange arm mechanism being used to replace the culture fluid in the culture dish (8), and isolation door members for the passage of the fluid exchange arm mechanism being provided on a plurality of the partitions (10); The rotating driving member comprises a turntable (17) horizontally rotatably connected to the bottom of the box body (1) and a second motor (23) fixedly connected to the bottom of the box body (1); a gear ring (25) is fixedly sleeved on the side wall of the turntable (17); a gear (24) is fixedly sleeved on the output shaft of the second motor (23); the gear (24) is meshed with the gear ring (25); the column (18) is vertically fixedly connected to the top of the turntable (17), and the column (18) and the turntable (17) are coaxially arranged; The liquid exchange arm mechanism comprises a liquid exchange arm (12) horizontally fixedly connected to the top of the side wall of the column (18); a groove is provided at the bottom of the liquid exchange arm (12); one end of a liquid exchange support arm (13) is hingedly connected to the side of the groove away from the column (18); the groove is used to receive the liquid exchange support arm (13); the hinge shaft of the liquid exchange support arm (13) is transmission-connected to a first motor (14); the first motor (14) is fixedly connected to the liquid exchange arm (12); The isolation door member comprises a through hole formed on the partition (10), the through hole being used to ensure that the fluid exchange arm (12) passes through, and an opening and closing partition (11) is hingedly connected to the top of the through hole, the opening and closing partition (11) closes the through hole when not pushed by the fluid exchange arm (12).

2. A tumor cell culture device according to claim 1, characterized in that: A horizontal partition plate (16) is fixedly connected to the inner side of the box body (1), the lifting platform (6) and the partition plate (10) are arranged above the horizontal partition plate (16), the rotating drive member is arranged below the horizontal partition plate (16), and the bottom of the column (18) passes through the horizontal partition plate (16) and is in transmission connection with the rotating drive member.

3. A tumor cell culture device according to claim 2, characterized in that: The lifting drive assembly comprises an electric telescopic rod (15) vertically fixedly connected to the top of the horizontal partition plate (16), and the telescopic end of the electric telescopic rod (15) is fixedly connected to the top of the lifting platform (6).

4. A tumor cell culture device according to claim 2, characterized in that: A plurality of guide columns (9) are slidably penetrated through the edge of the lifting platform (6), and the plurality of guide columns (9) are vertically fixedly connected to the horizontal partition plate (16).

5. The tumor cell culture device according to claim 1, characterized in that: A two-way liquid pump (22) is fixedly connected to the turntable (17); one connection port of the two-way liquid pump (22) is connected to one end of a first rubber hose; the other end of the first rubber hose is arranged corresponding to the culture dish (8) through the column (18), the liquid exchange arm (12) and the liquid exchange support arm (13); another connection port of the two-way liquid pump (22) is connected to one end of a second rubber hose; the other end of the second rubber hose extends out of the box (1).

6. The tumor cell culture device according to claim 1, characterized in that: A second through hole is provided in the center of the lifting platform (6), and a deflection platform (7) is rotatably connected in the second through hole. The deflection platform (7) is used to place a culture dish (8). Two groups of elastic bands (3) are fixedly connected to the bottom of the lifting platform (6), and the two groups of elastic bands (3) are respectively attached to the bottom of the deflection platform (7). The two groups of elastic bands (3) are located on both sides of the rotation axis of the deflection platform (7).

7. A tumor cell culture device according to claim 6, characterized in that: An iron rod (21) is fixedly connected to the bottom of the deflection table (7), a cylinder (19) is fixedly connected to the turntable (17), an electromagnet (20) is fixedly connected to the telescopic end of the cylinder (19), the electromagnet (20) is arranged corresponding to the iron rod (21), and the telescopic direction of the cylinder (19) is arranged perpendicular to the rotation axis direction of the deflection table (7).

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