A method for cultivating red nine-spined perch fry with high survival rate

Abstract

The invention discloses a method for cultivating red nine-spined perch fry with a high survival rate, and relates to the technical field of marine fish breeding. The cultivation method comprises the following steps: (1) regulating the light source environment: using a blue light source with a wavelength range of 420-450nm to irradiate the red nine-spined perch fry for 60-75 days during the growth process; (2) transforming the bottom environment: laying a layer of artificial materials on the bottom of the water, introducing a water body with a salinity of 30-35‰ as a breeding water body, and using an oxygen pump for aeration throughout the day; (3) feeding A feed and B feed alternately: first feed A feed for 8-10 days, then feed A feed for 8-10 days, and feed alternately until the juvenile stage; A feed is prepared by adding oxalis to the basic feed, and B feed is prepared by adding loperamide to the basic feed. By means of the three parallel measures of regulating the light source + bottom transformation + alternating feeding, it is beneficial to improve the survival rate of red nine-spined perch fry, thereby improving the economic benefits.

Description

Technical Field

[0001] The invention relates to the technical field of marine fish breeding, and in particular to a method for cultivating red nine-spined sea bass fry with a high survival rate. Background Art

[0002] Red ninethorn perch, ( Cephalopholis sonnerati ), commonly known as netted bream, cross-fish, grouper, red rudder, red melon seed spot, is a fish of the genus Nine-spined Perch of the family Serranidae, order Perciformes, order Perciformes, class Actinopterygii. The body is red, with dark brown reticulation on the head and front end of the back, without spots and stripes. There are no canine teeth in the middle of the lower jaw, and the anal fin has 3 spines and 9 fin rays. The rear edge of the caudal fin is round and has no stripes. It often lives in shallow seas with rocky bottoms, feeds on invertebrates and fish, and is a tropical coral reef and coastal fish. As a high-end edible fish on the existing market, the red nine-spined perch is deeply loved by consumers. At present, the population of wild red nine-spined perch has dropped significantly due to overfishing, and the artificial breeding technology of red nine-spined perch in my country is not yet mature, and there is little research on the artificial breeding method of red nine-spined perch fry. It has not been possible to carry out large-scale artificial breeding of red nine-spined perch fry, especially during the breeding process, the feeding rate of the fry is unstable, resulting in poor growth and development and reproductive capacity of the fry, and low survival rate, which cannot meet the market demand for red nine-spined perch. Therefore, providing a stable breeding method with a high survival rate for red nine-spined seabass fry and realizing large-scale breeding of artificially bred red nine-spined seabass have become important directions that need to be tackled in the aquaculture industry. Summary of the invention

[0003] In view of the above-mentioned defects of the prior art, the present invention proposes a method for cultivating red nine-spined sea bass fry with high survival rate to solve the problems raised by the above-mentioned background technology.

[0004] In order to achieve the above object, the present invention provides the following technical solutions:

[0005] A method for cultivating red nine-spined perch fry with high survival rate, the method comprising the following steps:

[0006] (1) Regulating the light source environment: During the growth of red nine-spined perch fry, a blue light source with a wavelength range of 420-450 nm was used for 60-75 days;

[0007] (2) Underwater environment transformation: lay a layer of artificial materials on the bottom of the water, introduce water with a salinity of 30-35‰ as aquaculture water, and use an oxygen pump for aeration throughout the day;

[0008] (3) Feeding feed A and feed B alternately: first feed feed A for 8-10 days, then feed feed A for 8-10 days, and feed alternately until the juvenile stage; feed A is prepared by adding Daphniphyllum calycinum Benth. A shrub of the genus Daphniphyllum in the family Daphniaceae to the basic feed, and feed B is prepared by adding loperamide to the basic feed;

[0009] The artificial material comprises, by weight, 12 to 20 parts of oyster shell powder-kaolin modified material, 10 to 15 parts of sodium silicate, 5 to 8 parts of superphosphate, and 4 to 10 parts of Clostridium butyricum metabolites.

[0010] Preferably, the breeding density of red nine-thorn perch fry is 100-200 tails / m 2 .

[0011] Preferably, the blue light source is placed 80 to 100 cm above the aquaculture water body.

[0012] Preferably, the light intensity of the blue light source in step (1) is 100-150 lux, and the light cycle is 17L:7D.

[0013] Preferably, the artificial material in step (2) comprises, by weight, 15 parts of oyster shell powder-kaolin modified material, 12 parts of sodium silicate, 6 parts of superphosphate, and 5 parts of Clostridium butyricum metabolites.

[0014] Preferably, Clostridium butyricum is purchased from Shanghai Yiyan Biotechnology Co., Ltd.

[0015] Preferably, the thickness of the artificial material laid in step (2) is 3 to 6 cm.

[0016] Preferably, the preparation method of the oyster shell powder-kaolin modified material in step (2) is: in parts by weight, 2 to 6 parts of oyster shell powder and 5 to 10 parts of kaolin are added to 10 to 20 parts of anhydrous ethanol, and after standing for 10 to 15 minutes, 1 to 3 parts of hexadecyltrimethoxysilane are added, and the mixture is heated to 75 to 82° C. while stirring, and then ultrasonically vibrated for 10 to 20 minutes, filtered, washed with water, and dried to obtain the modified material.

[0017] Preferably, the preparation method of the Clostridium butyricum metabolite in step (2) is as follows: after activating Clostridium butyricum, inoculate it into a reinforced Clostridium culture medium, and culture it anaerobically at a temperature of 30-35°C for 12-16 hours to obtain a bacterial solution; take 1% of the bacterial solution and inoculate it into LB liquid culture medium, and culture it at a temperature of 28-32°C and a rotation speed of 220-260 rpm for 5-8 hours, centrifuge it, and collect the supernatant to obtain the Clostridium butyricum metabolite.

[0018] Preferably, the reinforced Clostridium medium (RCM medium): 3g yeast extract, 10g beef extract, 5g sodium chloride, 3g sodium acetate trihydrate, 0.5g cysteine ​​hydrochloride, 0.2ml 0.5% methylene blue, 1000ml distilled water; adjust the pH value to 7.1±0.1, sterilize at 115°C for 20min and set aside.

[0019] Preferably, LB (Luria-Bertani) liquid culture medium (lysing broth medium): peptone 10g, yeast powder 5g, NaCL 10g, add water to 1000ml, adjust the pH to 7.2-7.4 with NaOH or HCL, sterilize at 115°C for 30min for use.

[0020] Preferably, the preparation method of the artificial material in step (2) is: mixing oyster shell powder-kaolin modified material, sodium silicate and superphosphate to obtain a mixture; adding 2 to 5 times the volume of anhydrous ethanol to the mixture, performing a first ultrasonic oscillation, fully ball milling, and then adding the metabolites of Clostridium butyricum, and performing a second ultrasonic oscillation to obtain the artificial material.

[0021] Preferably, the first ultrasonic oscillation frequency is 20-25 kHz, and the time is 12-20 min; the second ultrasonic oscillation frequency is 30-40 kHz, and the time is 5-10 min.

[0022] Preferably, in step (3), the amount of nelumbinis and loperamide added to feed A and feed B is 5-9wt%, the feeding frequency is 3 times / d, and the daily feeding amount is 3-6% of the fry body weight.

[0023] Preferably, the basic feed is 35% white fish meal, 15% soybean meal, 4% shrimp skin, 4% soybean lecithin, 30.2% protein powder, 2.68% fish oil, 1.32% soybean oil, 6.5% starch, 1% choline, 0.1% Vino multivitamin for fish, 0.1% Vino mycin and multimineral, and 0.1% Vino compound probiotics.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] (1) The present invention utilizes a blue light source of a specific spectrum to regulate the light environment of red nine-spined perch fry, thereby meeting the red nine-spined perch's demand for light, improving the problem of uneven specifications of the red nine-spined perch during growth, and making the growth rate of the red nine-spined perch slightly different within the same period of time, thereby effectively improving the economic benefits of red nine-spined perch farming.

[0026] (2) The underwater artificial material is prepared using oyster shell powder-kaolin modified materials, sodium silicate, superphosphate and Clostridium butyricum metabolites. Oyster shell powder and kaolin have adsorption properties, which can achieve the adsorption and removal of phosphate and ammonia nitrogen in the water body. Because the modified material is placed at the bottom of the water, the increase in permeability will lead to a decrease in its adsorption of harmful substances in the water body. The technical solution uses hexadecyl trimethoxysilane as a modifier to hydrophobically modify the oyster shell powder-kaolin to improve the adsorption property, and cooperates with sodium silicate, superphosphate and Clostridium butyricum metabolites to purify the water body, improve the water environment, and help improve the survival rate of red nine-spined perch fry; and Clostridium butyricum metabolites can promote the growth of probiotics in the intestinal tract of fish fry, inhibit harmful bacteria, correct bacterial flora disorders, reduce toxin production, and regulate the microecological balance of the fish intestine, thereby shortening the breeding time of red nine-spined perch fry and reducing breeding costs.

[0027] (3) The present invention feeds feed A and feed B alternately, adds Chinese medicine to feed A, and adds western medicine to feed B. The Chinese medicine ingredients are consistent with the activity of the fish body and can be fully absorbed and utilized by the body. No drug residue will be produced in the body, thus avoiding the problem of drug residue. Western medicine takes effect quickly and can quickly promote the fish body to digest food and absorb nutrients. The combined use of Chinese medicine and western medicine can produce a synergistic effect, regulate the body's immunity, and improve the problem of low yield of red nine-spined sea bass. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the hatching period of red nine-spined perch;

[0029] Figure 2 This is a schematic diagram of the juvenile stage of red nine-spined perch;

[0030] Figure 3 This is a schematic diagram of the adult stage of red nine-spined perch. DETAILED DESCRIPTION

[0031] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0032] Example 1

[0033] According to the existing indoor cultivation and breeding methods, the water quality indicators are controlled as follows: pH 7.5~7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, nitrite ≤0.05mg / L; red nine-spined sea bass fry are bred according to the following regulation method.

[0034] A method for cultivating red nine-spined perch fry with high survival rate, the method comprising the following steps:

[0035] (1) Regulating the light source environment: During the growth of red nine-spined perch fry, a blue light source with a wavelength range of 420nm and a light intensity of 100lux was used for 60 days, with a photoperiod of 17L:7D. The blue light source was placed 80cm above the aquaculture water body;

[0036] (2) Underwater environment transformation: Lay a layer of artificial materials with a thickness of 3 to 6 cm on the bottom of the water, introduce water with a salinity of 30‰ as aquaculture water, and use an oxygen pump for aeration throughout the day;

[0037] (3) Feed A and feed B were fed alternately: feed A was fed for 8 days first, and then feed A for 8 days, with the feeding frequency of 3 times / day, at 09:00, 13:00 and 18:00 respectively, and the daily feeding amount was 3% of the fry body weight, and the feed was fed alternately until the juvenile stage; feed A was prepared by adding 5wt% of Acer truncatum to the basic feed, and feed B was prepared by adding 5wt% of loperamide to the basic feed;

[0038] The artificial material comprises, by weight, 12 parts of oyster shell powder-kaolin modified material, 10 parts of sodium silicate, 5 parts of superphosphate, and 4 parts of Clostridium butyricum metabolites.

[0039] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 2 parts of oyster shell powder and 5 parts of kaolin are added to 10 parts of anhydrous ethanol, and after standing for 10 minutes, 1 part of hexadecyltrimethoxysilane is added, and the mixture is heated to 75°C while stirring, and then ultrasonically vibrated for 10 minutes, filtered, washed with water, and dried to obtain the modified material.

[0040] Among them, the preparation method of Clostridium butyricum metabolites is as follows: after Clostridium butyricum is activated, it is inoculated into a reinforced Clostridium culture medium, and anaerobically cultured at a temperature of 30°C for 12 hours to obtain a bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and shaken and cultured at a temperature of 28°C and a speed of 220 rpm for 5 hours, centrifuged, and the supernatant is collected to obtain the Clostridium butyricum metabolites.

[0041] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; anhydrous ethanol with a volume twice that of the mixture is added, ultrasonic vibration is performed at a frequency of 20 kHz for 12 minutes, ball milling is performed thoroughly, and then the metabolites of Clostridium butyricum are added, and ultrasonic vibration is performed at a frequency of 30 kHz for 5 minutes for a second time to obtain the artificial material.

[0042] Example 2

[0043] According to the existing indoor cultivation and breeding methods, the water quality indicators are controlled as follows: pH 7.5~7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, nitrite ≤0.05mg / L; red nine-spined sea bass fry are bred according to the following regulation method.

[0044] A method for cultivating red nine-spined perch fry with high survival rate, the method comprising the following steps:

[0045] (1) Regulating the light source environment: During the growth of red nine-spined perch fry, a blue light source with a wavelength range of 450nm and a light intensity of 150lux was used for 75 days, with a photoperiod of 17L:7D. The blue light source was placed 100cm above the aquaculture water body;

[0046] (2) Underwater environment transformation: Lay a layer of artificial materials with a thickness of 3 to 6 cm on the bottom of the water, introduce water with a salinity of 35‰ as aquaculture water, and use an oxygen pump for aeration throughout the day;

[0047] (3) Feed A and feed B were fed alternately: feed A was fed for 10 days first, and then feed A for 10 days, with the feeding frequency of 3 times / day, at 09:00, 13:00 and 18:00 respectively, and the daily feeding amount was 6% of the fry body weight, and the feeds were fed alternately until the juvenile stage; feed A was prepared by adding 9wt% of Acer truncatum to the basic feed, and feed B was prepared by adding 9wt% of loperamide to the basic feed;

[0048] The artificial material comprises, by weight, 20 parts of oyster shell powder-kaolin modified material, 15 parts of sodium silicate, 8 parts of superphosphate, and 10 parts of Clostridium butyricum metabolites.

[0049] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 6 parts of oyster shell powder and 10 parts of kaolin are added to 20 parts of anhydrous ethanol, and after standing for 15 minutes, 3 parts of hexadecyltrimethoxysilane are added, and the mixture is heated to 82° C. while stirring, and then ultrasonically vibrated for 20 minutes, filtered, washed with water, and dried to obtain the modified material.

[0050] The preparation method of the metabolites of Clostridium butyricum is as follows: after activating Clostridium butyricum, it is inoculated into a reinforced Clostridium culture medium, and anaerobically cultured at a temperature of 35°C for 16 hours to obtain a bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and shaken and cultured at a temperature of 32°C and a speed of 260 rpm for 8 hours, centrifuged, and the supernatant is collected to obtain the metabolites of Clostridium butyricum.

[0051] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 5 times the volume of anhydrous ethanol is added to the mixture, ultrasonic vibration is performed at a frequency of 25 kHz for 20 minutes, and the mixture is fully ball-milled; then, the metabolites of Clostridium butyricum are added, and ultrasonic vibration is performed at a frequency of 40 kHz for 10 minutes to obtain the artificial material.

[0052] Example 3

[0053] According to the existing indoor cultivation and breeding methods, the water quality indicators are controlled as follows: pH 7.5~7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, nitrite ≤0.05mg / L; red nine-spined sea bass fry are bred according to the following regulation method.

[0054] A method for cultivating red nine-spined perch fry with high survival rate, the method comprising the following steps:

[0055] (1) Regulating the light source environment: During the growth of red nine-spined perch fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the aquaculture water body;

[0056] (2) Underwater environment transformation: Lay a layer of artificial materials with a thickness of 3 to 6 cm on the bottom of the water, introduce water with a salinity of 32‰ as aquaculture water, and use an oxygen pump for aeration throughout the day;

[0057] (3) Feeding A and B alternately: Feed A for 9 days first, then feed A for 9 days, 3 times / day, at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 5% of the fry body weight, and feeding alternately until the juvenile stage; Feed A was prepared by adding 7wt% of Acer truncatum to the basic feed, and Feed B was prepared by adding 7wt% of Loperamide to the basic feed;

[0058] The artificial material comprises, by weight, 15 parts of oyster shell powder-kaolin modified material, 12 parts of sodium silicate, 6 parts of superphosphate, and 5 parts of Clostridium butyricum metabolites.

[0059] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 5 parts of oyster shell powder and 7 parts of kaolin are added to 15 parts of anhydrous ethanol, and after standing for 13 minutes, 2 parts of hexadecyltrimethoxysilane are added, and the mixture is heated to 80° C. while stirring, and then ultrasonically vibrated for 15 minutes, filtered, washed with water, and dried to obtain the modified material.

[0060] Among them, the preparation method of Clostridium butyricum metabolites is as follows: after Clostridium butyricum is activated, it is inoculated into a reinforced Clostridium culture medium, and anaerobically cultured at a temperature of 32°C for 15 hours to obtain a bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and shaken and cultured at a temperature of 30°C and a speed of 250 rpm for 7 hours, centrifuged, and the supernatant is collected to obtain the Clostridium butyricum metabolites.

[0061] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 4 times the volume of anhydrous ethanol is added to the mixture, ultrasonic vibration is performed for 18 minutes at a frequency of 22 kHz for the first time, and the mixture is fully ball-milled; then the metabolites of Clostridium butyricum are added, and ultrasonic vibration is performed for 9 minutes at a frequency of 35 kHz for the second time to obtain the artificial material.

[0062] Comparative Example 1

[0063] The difference between this comparative example and Example 3 is that the blue light source is replaced by a white LED lamp, and the other components and steps remain unchanged.

[0064] According to the existing indoor cultivation and breeding methods, the water quality indicators are controlled as follows: pH 7.5~7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, nitrite ≤0.05mg / L; red nine-spined sea bass fry are bred according to the following regulation method.

[0065] A method for cultivating red nine-spined perch fry with high survival rate, the method comprising the following steps:

[0066] (1) Regulating the light source environment: During the growth of red nine-spined perch fry, a white light source with a wavelength range of 450nm and a light intensity of 120lux was used for 70 days, with a photoperiod of 17L:7D; the white light source was placed 90cm above the aquaculture water body;

[0067] (2) Underwater environment transformation: Lay a layer of artificial materials with a thickness of 3 to 6 cm on the bottom of the water, introduce water with a salinity of 32‰ as aquaculture water, and use an oxygen pump for aeration throughout the day;

[0068] (3) Feeding A and B alternately: Feed A for 9 days first, then feed A for 9 days, 3 times / day, at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 5% of the fry body weight, and feeding alternately until the juvenile stage; Feed A was prepared by adding 7wt% of Acer truncatum to the basic feed, and Feed B was prepared by adding 7wt% of Loperamide to the basic feed;

[0069] The artificial material comprises, by weight, 15 parts of oyster shell powder-kaolin modified material, 12 parts of sodium silicate, 6 parts of superphosphate, and 5 parts of Clostridium butyricum metabolites.

[0070] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 5 parts of oyster shell powder and 7 parts of kaolin are added to 15 parts of anhydrous ethanol, and after standing for 13 minutes, 2 parts of hexadecyltrimethoxysilane are added, and the mixture is heated to 80° C. while stirring, and then ultrasonically vibrated for 15 minutes, filtered, washed with water, and dried to obtain the modified material.

[0071] Among them, the preparation method of Clostridium butyricum metabolites is as follows: after Clostridium butyricum is activated, it is inoculated into a reinforced Clostridium culture medium, and anaerobically cultured at a temperature of 32°C for 15 hours to obtain a bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and shaken and cultured at a temperature of 30°C and a speed of 250 rpm for 7 hours, centrifuged, and the supernatant is collected to obtain the Clostridium butyricum metabolites.

[0072] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 4 times the volume of anhydrous ethanol is added to the mixture, ultrasonic vibration is performed for 18 minutes at a frequency of 22 kHz for the first time, and the mixture is fully ball-milled; then the metabolites of Clostridium butyricum are added, and ultrasonic vibration is performed for 9 minutes at a frequency of 35 kHz for the second time to obtain the artificial material.

[0073] Comparative Example 2

[0074] The difference between this comparative example and Example 3 is that no artificial material is laid, and the other components and steps remain unchanged.

[0075] According to the existing indoor cultivation and breeding methods, the water quality indicators are controlled as follows: pH 7.5~7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, nitrite ≤0.05mg / L; red nine-spined sea bass fry are bred according to the following regulation method.

[0076] A method for cultivating red nine-spined perch fry with high survival rate, the method comprising the following steps:

[0077] (1) Regulating the light source environment: During the growth of red nine-spined perch fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the aquaculture water body;

[0078] (2) Feed A and feed B were fed alternately: feed A was fed for 9 days first, and then feed A for 9 days. The feeding frequency was 3 times / d, at 09:00, 13:00 and 18:00 respectively. The daily feeding amount was 5% of the body weight of the fry. Feeding was alternated until the juvenile stage. Feed A was prepared by adding 7wt% of Achyranthes bidentata to the basic feed, and feed B was prepared by adding 7wt% of loperamide to the basic feed.

[0079] Comparative Example 3

[0080] The difference between this comparative example and Example 3 is that the oyster shell powder-kaolin in the artificial material is not modified, and the other components and steps remain unchanged.

[0081] According to the existing indoor cultivation and breeding methods, the water quality indicators are controlled as follows: pH 7.5~7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, nitrite ≤0.05mg / L; red nine-spined sea bass fry are bred according to the following regulation method.

[0082] A method for cultivating red nine-spined perch fry with high survival rate, the method comprising the following steps:

[0083] (1) Regulating the light source environment: During the growth of red nine-spined perch fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the aquaculture water body;

[0084] (2) Underwater environment transformation: Lay a layer of artificial materials with a thickness of 3 to 6 cm on the bottom of the water, introduce water with a salinity of 32‰ as aquaculture water, and use an oxygen pump for aeration throughout the day;

[0085] (3) Feeding A and B alternately: Feed A for 9 days first, then feed A for 9 days, 3 times / day, at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 5% of the fry body weight, and feeding alternately until the juvenile stage; Feed A was prepared by adding 7wt% of Acer truncatum to the basic feed, and Feed B was prepared by adding 7wt% of Loperamide to the basic feed;

[0086] The artificial material comprises, by weight, 15 parts of oyster shell powder-kaolin mixed material, 12 parts of sodium silicate, 6 parts of superphosphate, and 5 parts of Clostridium butyricum metabolites.

[0087] Among them, the preparation method of Clostridium butyricum metabolites is as follows: after Clostridium butyricum is activated, it is inoculated into a reinforced Clostridium culture medium, and anaerobically cultured at a temperature of 32°C for 15 hours to obtain a bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and shaken and cultured at a temperature of 30°C and a speed of 250 rpm for 7 hours, centrifuged, and the supernatant is collected to obtain the Clostridium butyricum metabolites.

[0088] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin mixed material, sodium silicate and superphosphate are mixed to obtain a mixture; 4 times the volume of anhydrous ethanol is added to the mixture, ultrasonic vibration is performed at a frequency of 22 kHz for 18 minutes, and the mixture is fully ball-milled; then, the metabolites of Clostridium butyricum are added, and ultrasonic vibration is performed at a frequency of 35 kHz for 9 minutes to obtain the artificial material.

[0089] Comparative Example 4

[0090] The difference between this comparative example and Example 3 is that the metabolites of Clostridium butyricum in the artificial material are replaced with metabolites of lactic acid bacteria, and the other components and steps remain unchanged.

[0091] According to the existing indoor cultivation and breeding methods, the water quality indicators are controlled as follows: pH 7.5~7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, nitrite ≤0.05mg / L; red nine-spined sea bass fry are bred according to the following regulation method.

[0092] A method for cultivating red nine-spined perch fry with high survival rate, the method comprising the following steps:

[0093] (1) Regulating the light source environment: During the growth of red nine-spined perch fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the aquaculture water body;

[0094] (2) Underwater environment transformation: Lay a layer of artificial materials with a thickness of 3 to 6 cm on the bottom of the water, introduce water with a salinity of 32‰ as aquaculture water, and use an oxygen pump for aeration throughout the day;

[0095] (3) Feeding A and B alternately: Feed A for 9 days first, then feed A for 9 days, 3 times / day, at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 5% of the fry body weight, and feeding alternately until the juvenile stage; Feed A was prepared by adding 7wt% of Acer truncatum to the basic feed, and Feed B was prepared by adding 7wt% of Loperamide to the basic feed;

[0096] The artificial material comprises, by weight, 15 parts of oyster shell powder-kaolin modified material, 12 parts of sodium silicate, 6 parts of superphosphate, and 5 parts of lactic acid bacteria metabolites.

[0097] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 5 parts of oyster shell powder and 7 parts of kaolin are added to 15 parts of anhydrous ethanol, and after standing for 13 minutes, 2 parts of hexadecyltrimethoxysilane are added, and the mixture is heated to 80° C. while stirring, and then ultrasonically vibrated for 15 minutes, filtered, washed with water, and dried to obtain the modified material.

[0098] Among them, the preparation method of lactic acid bacteria metabolites is as follows: after the lactic acid bacteria are activated, they are inoculated into an agar medium, and anaerobically cultured at a temperature of 32°C for 15 hours to obtain a bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and shaken and cultured at a temperature of 30°C and a speed of 250 rpm for 7 hours, centrifuged, and the supernatant is collected to obtain the lactic acid bacteria metabolites.

[0099] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 4 times the volume of anhydrous ethanol is added to the mixture, ultrasonic vibration is performed at a frequency of 22 kHz for 18 minutes, and the mixture is fully ball-milled; then, lactic acid bacteria metabolites are added, and ultrasonic vibration is performed at a frequency of 35 kHz for 9 minutes to obtain the artificial material.

[0100] Comparative Example 5

[0101] The difference between this comparative example and Example 3 is that the feed for feeding fry is all basic feed, no nephrolepis and loperamide are added, and the other ingredients and steps remain unchanged.

[0102] According to the existing indoor cultivation and breeding methods, the water quality indicators are controlled as follows: pH 7.5~7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, nitrite ≤0.05mg / L; red nine-spined sea bass fry are bred according to the following regulation method.

[0103] A method for cultivating red nine-spined perch fry with high survival rate, the method comprising the following steps:

[0104] (1) Regulating the light source environment: During the growth of red nine-spined perch fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the aquaculture water body;

[0105] (2) Underwater environment transformation: Lay a layer of artificial materials with a thickness of 3 to 6 cm on the bottom of the water, introduce water with a salinity of 32‰ as aquaculture water, and use an oxygen pump for aeration throughout the day;

[0106] (3) Feeding basic feed: feeding frequency is 3 times / d, at 09:00, 13:00 and 18:00 respectively, and the daily feeding amount is 5% of the fry body weight, and feeding is alternately continued until the juvenile stage; the basic feed is 35% white fish meal, 15% soybean meal, 4% shrimp skin, 4% soybean lecithin, 30.2% protein powder, 2.68% fish oil, 1.32% soybean oil, 6.5% starch, 1% choline, 0.1% Vino fish multivitamin, 0.1% Vino mycobalamin, and 0.1% Vino compound probiotics;

[0107] The artificial material comprises, by weight, 15 parts of oyster shell powder-kaolin modified material, 12 parts of sodium silicate, 6 parts of superphosphate, and 5 parts of Clostridium butyricum metabolites.

[0108] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 5 parts of oyster shell powder and 7 parts of kaolin are added to 15 parts of anhydrous ethanol, and after standing for 13 minutes, 2 parts of hexadecyltrimethoxysilane are added, and the mixture is heated to 80° C. while stirring, and then ultrasonically vibrated for 15 minutes, filtered, washed with water, and dried to obtain the modified material.

[0109] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 4 times the volume of anhydrous ethanol is added to the mixture, ultrasonic vibration is performed for 18 minutes at a frequency of 22 kHz for the first time, and the mixture is fully ball-milled; then the metabolites of Clostridium butyricum are added, and ultrasonic vibration is performed for 9 minutes at a frequency of 35 kHz for the second time to obtain the artificial material.

[0110] The preparation method of the Clostridium butyricum metabolite is as follows: after activating Clostridium butyricum, it is inoculated into a reinforced Clostridium culture medium, and anaerobically cultured at a temperature of 32°C for 15 hours to obtain a bacterial solution; 1% of the bacterial solution is inoculated into an LB liquid culture medium, and shaken and cultured at a temperature of 30°C and a speed of 250 rpm for 7 hours, centrifuged, and the supernatant is collected to obtain the Clostridium butyricum metabolite.

[0111] Comparative Example 6

[0112] The difference between this comparative example and Example 3 is that the fry feeding feed is not fed alternately, and the other ingredients and steps remain unchanged.

[0113] According to the existing indoor cultivation and breeding methods, the water quality indicators are controlled as follows: pH 7.5~7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, nitrite ≤0.05mg / L; red nine-spined sea bass fry are bred according to the following regulation method.

[0114] A method for cultivating red nine-spined perch fry with high survival rate, the method comprising the following steps:

[0115] (1) Regulating the light source environment: During the growth of red nine-spined perch fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the aquaculture water body;

[0116] (2) Underwater environment transformation: Lay a layer of artificial materials with a thickness of 3 to 6 cm on the bottom of the water, introduce water with a salinity of 32‰ as aquaculture water, and use an oxygen pump for aeration throughout the day;

[0117] (3) Feeding a mixed feed of feed A + feed B: feeding frequency 3 times / d, at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 5% of the fry body weight, and feeding alternately until the juvenile stage; the mixed feed was prepared by adding 7wt% of neem and 7wt% of loperamide to the basic feed;

[0118] The artificial material comprises, by weight, 15 parts of oyster shell powder-kaolin modified material, 12 parts of sodium silicate, 6 parts of superphosphate, and 5 parts of Clostridium butyricum metabolites.

[0119] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 5 parts of oyster shell powder and 7 parts of kaolin are added to 15 parts of anhydrous ethanol, and after standing for 13 minutes, 2 parts of hexadecyltrimethoxysilane are added, and the mixture is heated to 80° C. while stirring, and then ultrasonically vibrated for 15 minutes, filtered, washed with water, and dried to obtain the modified material.

[0120] Among them, the preparation method of Clostridium butyricum metabolites is as follows: after Clostridium butyricum is activated, it is inoculated into a reinforced Clostridium culture medium, and anaerobically cultured at a temperature of 32°C for 15 hours to obtain a bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and shaken and cultured at a temperature of 30°C and a speed of 250 rpm for 7 hours, centrifuged, and the supernatant is collected to obtain the Clostridium butyricum metabolites.

[0121] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 4 times the volume of anhydrous ethanol is added to the mixture, ultrasonic vibration is performed for 18 minutes at a frequency of 22 kHz for the first time, and the mixture is fully ball-milled; then the metabolites of Clostridium butyricum are added, and ultrasonic vibration is performed for 9 minutes at a frequency of 35 kHz for the second time to obtain the artificial material.

[0122] Experimental Example 1: Survival rate of fry

[0123] The methods of Examples 1 to 3 and Comparative Examples 1 to 6 were used to culture red nine-spined perch fry, and the number of stocked and harvested perch fry was counted to calculate the survival rate, where the survival rate = the number of harvested perch fry / the number of stocked perch fry. The results are shown in Table 1:

[0124] Table 1 Survival rate of fry culture

[0125]

[0126] As can be seen from Table 1, the survival rate of red nine-spined sea bass fry cultured by the methods of Examples 1 to 3 is high (e.g. Figure 1 and Figure 2As shown), up to 98.0% and above. Comparing Example 3 with Comparative Example 1, it can be seen that after changing to a white light source, the survival rate of the fry decreased, indicating that the use of the blue light source of the technical solution can improve the survival rate of the fry. The blue light source can simulate the light conditions in the natural environment, provide a comfortable and safe growth environment for fish, promote the growth of small fish, and make muscle cells grow stronger; Comparing Example 3 with Comparative Examples 2 to 4, it can be seen that Comparative Example 2 does not lay artificial materials, the oyster shell powder-kaolin in the artificial materials of Comparative Example 3 is not modified, and lactic acid bacteria are used instead of Clostridium butyricum in Comparative Example 4. The survival rate of red nine-spined sea bass fry has decreased significantly, It shows that the metabolites of Clostridium butyricum have specificity in improving the immunity of red nine-spined perch fry. The metabolites obtained by replacing probiotic strains with similar functions have a significantly lower degree of improvement in its immunity. The oyster shell powder-kaolin modified material cooperates with sodium silicate, superphosphate and Clostridium butyricum metabolites to purify the water body, improve the water environment, and help improve the survival rate of red nine-spined perch fry; if the oyster shell powder-kaolin is not modified, the adsorption effect of the oyster shell powder-kaolin mixed material on harmful substances in the water body will be reduced, which is easy to cause turbidity of the water body, reduce the oxygen content of the water body, and thus reduce the survival rate of the fry. By comparing Example 3 with Comparative Examples 5 and 6, it can be seen that the addition of Niu Er Feng and loperamide in ordinary feed is beneficial to improve the feeding rate of fry, enhance physical fitness, enhance the immune ability of fish organisms through the synergistic effect of Chinese and Western medicines, and improve the survival rate after breeding.

[0127] Experimental Example 2: Weight and Total Length Test

[0128] The weight and total length of red nine-spined perch fry were measured during the breeding period, and the growth performance of red nine-spined perch fry was evaluated. 50 red nine-spined perch fry were randomly selected, and the weight and total length of red nine-spined perch fry at 30d, 60d and 90d were measured using a weighing scale with an accuracy of 0.01g and a ruler with an accuracy of 0.01mm. The application number CN202311642700.6, a factory-based artificial breeding method for red nine-spined perch fry, was used as conventional breeding, and the obtained fry were used as the control group. The results are shown in Table 2:

[0129] Table 2 Body weight and total length test results

[0130]

[0131] It can be seen from Table 2 that the method for breeding nine-spined perch fry of the present invention can increase the weight and total length of fish and grow faster than conventional breeding; and it can be seen from the data of Examples 1 to 3 that the fry obtained by using the method for breeding nine-spined perch fry of the present invention are of uniform specifications, and the fry of uniform specifications are more consistent in competing for food and growth rate, reducing the competition and survival pressure caused by individual differences, thereby improving the overall survival rate.

[0132] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for cultivating red nine-spined perch fry with high survival rate, characterized in that: The cultivation method comprises the following steps: (1) Regulating the light source environment: During the growth of red nine-spined perch fry, a blue light source with a wavelength range of 420-450 nm was used for 60-75 days; (2) Underwater environment transformation: lay a layer of artificial materials on the bottom of the water, introduce water with a salinity of 30-35‰ as aquaculture water, and use an oxygen pump for aeration throughout the day; (3) Alternating feeding of feed A and feed B: Alternating feeding until the juvenile stage; feed A is prepared by adding smilax glabra to the basic feed, and feed B is prepared by adding loperamide to the basic feed; The artificial material comprises, by weight, 12 to 20 parts of oyster shell powder-kaolin modified material, 10 to 15 parts of sodium silicate, 5 to 8 parts of superphosphate, and 4 to 10 parts of Clostridium butyricum metabolites; Step (2) The thickness of the artificial material to be laid is 3 to 6 cm; The preparation method of the oyster shell powder-kaolin modified material is as follows: by weight, 2-6 parts of oyster shell powder and 5-10 parts of kaolin are added to 10-20 parts of anhydrous ethanol, and after standing for 10-15 minutes, 1-3 parts of hexadecyltrimethoxysilane are added, and the mixture is heated to 75-82° C. while stirring, and then ultrasonically vibrated for 10-20 minutes, filtered, washed with water, and dried to obtain the modified material; The preparation method of the Clostridium butyricum metabolite is as follows: after activating Clostridium butyricum, the Clostridium butyricum is inoculated into a reinforced Clostridium culture medium, and anaerobically cultured at a temperature of 30-35° C. for 12-16 hours to obtain a bacterial solution; 1% of the bacterial solution is inoculated into an LB liquid culture medium, and cultured at a temperature of 28-32° C. and a rotation speed of 220-260 rpm for 5-8 hours, centrifuged, and the supernatant is collected to obtain the Clostridium butyricum metabolite; The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 2 to 5 times the volume of anhydrous ethanol is added to the mixture, ultrasonic vibration is performed for the first time, ball milling is performed sufficiently, and then the metabolite of Clostridium butyricum is added, and ultrasonic vibration is performed for the second time to obtain the artificial material; The first ultrasonic oscillation frequency is 20-25kHz, and the time is 12-20min; the second ultrasonic oscillation frequency is 30-40kHz, and the time is 5-10min; In step (3), the amount of nephrolepis oxyphylla and loperamide added to feed A and feed B is 5-9wt%, the feeding frequency is 3 times / d, and the daily feeding amount is 3-6% of the fry body weight.

2. The method for cultivating red nine-spined sea bass fry with high survival rate according to claim 1, characterized in that: The blue light source is placed 80~100cm above the aquaculture water.

3. The method for cultivating red nine-spined sea bass fry with high survival rate according to claim 1, characterized in that: The light intensity of the blue light source in step (1) is 100-150 lux, and the light cycle is 17L:7D.

4. The method for cultivating red nine-spined sea bass fry with high survival rate according to claim 1, characterized in that: The artificial material in step (2) comprises, by weight, 15 parts of oyster shell powder-kaolin modified material, 12 parts of sodium silicate, 6 parts of superphosphate, and 5 parts of Clostridium butyricum metabolites.

Images