Skin-whitening and anti-aging plant extracts, their preparation methods and applications

Abstract

The present invention discloses a plant extract for skin whitening and anti-aging, its preparation method and application. The plant extract is composed of the following raw materials in parts by mass: 3 to 30 parts of Salvia miltiorrhiza, 4 to 40 parts of Angelica sinensis, 3 to 30 parts of Atractylodes macrocephala, 3 to 30 parts of Dioscorea opposita, 4 to 40 parts of Rehmannia glutinosa, 3 to 30 parts of Morus alba root bark, 2 to 20 parts of Pueraria lobata, 1 to 10 parts of Coptis chinensis, 1 to 10 parts of Scutellaria baicalensis, and 1 to 10 parts of Phellodendron amurense. Based on the theory of yin and yang in traditional Chinese medicine, the present invention uses Salvia miltiorrhiza and Coptis chinensis to enter the heart, Angelica sinensis and Scutellaria baicalensis to enter the liver, Morus alba root bark and Dioscorea opposita to enter the lung, Atractylodes macrocephala and Pueraria lobata to enter the spleen, and Rehmannia glutinosa and Phellodendron amurense to enter the kidney, so that the active ingredients can be absorbed by the skin, enter the heart, liver, spleen, lung and kidney, regulate the balance of yin and yang, can take into account skin whitening and anti-aging, has low raw material cost and mild drug effect.

Description

Technical Field

[0001] The present invention relates to the technical field of daily-use cosmetics, and particularly relates to a skin-whitening and anti-aging plant extract, a preparation method thereof, and an application thereof. Background Art

[0002] Traditional Chinese medicine theory emphasizes that skin health is closely related to the balance of yin and yang in the body. The imbalance of yin and yang is one of the roots of skin problems, among which yin deficiency and yang deficiency are the main influencing factors. Yin deficiency refers to the deficiency of yin fluid in the body, which cannot moisten and nourish the skin. It is usually manifested as dry skin, lack of luster, dullness, and roughness, and may also lead to a decrease in skin elasticity, the appearance of fine lines and wrinkles. Yang deficiency refers to the deficiency of yang qi in the body, which cannot maintain the normal functions of the skin. It is usually manifested as loose skin, lack of luster, dull color, and may also lead to a decrease in the vitality of skin cells, loss of elasticity and firmness. Therefore, developing pure traditional Chinese medicine skin care products in combination with traditional Chinese medicine theory to regulate the balance of yin and yang in the body can achieve the effect of beautifying the skin and maintaining youth.

[0003] However, most of the so-called traditional Chinese medicine skin care products on the market mainly contain chemical components, and only one or a few traditional Chinese medicines are used as auxiliary components, which are not real traditional Chinese medicine skin care products. And among the few skin care products with pure traditional Chinese medicine as components, precious medicinal materials or multiple potent medicines are used in combination, resulting in increased product costs and enhanced irritation. Moreover, the improper compatibility of traditional Chinese medicines, inaccurate dosage, and failure to extract effective components in current traditional Chinese medicine skin care products lead to poor product efficacy. Summary of the Invention

[0004] The present invention provides a skin-whitening and anti-aging plant extract, a preparation method thereof, and an application thereof, so as to solve the technical problems of high cost, mild medicinal effect, and poor efficacy of current pure traditional Chinese medicine skin care products.

[0005] To solve the above technical problems, in the first aspect, the present invention provides a skin-whitening and anti-aging plant extract, which is composed of the following raw materials in parts by mass:

[0006] Salvia miltiorrhiza 3 parts to 30 parts, Angelica sinensis 4 parts to 40 parts, Atractylodes macrocephala 3 parts to 30 parts, Dioscorea opposita 3 parts to 30 parts, Rehmannia glutinosa 4 parts to 40 parts, Mori cortex 3 parts to 30 parts, Pueraria lobata 2 parts to 20 parts, Coptis chinensis 1 part to 10 parts, Scutellaria baicalensis 1 part to 10 parts, Phellodendron amurense 1 part to 10 parts;

[0007] The preparation method of the skin-whitening and anti-aging plant extract includes the following steps:

[0008] Step 1, weigh each raw material component according to parts by mass, crush to 20 mesh to 60 mesh, and wash;

[0009] Step 2: Add 50% - 90% ethanol aqueous solution to the raw material components. After soaking for 12h - 24h, add 20 - 30 times the total mass of the raw material components of water, heat to 88°C - 95°C while stirring, keep stirring for 70min - 80min, and then filter the filtrate with an ultrafilter;

[0010] Step 3: Mix and evaporate and concentrate the filtrate until the concentration reaches 8% - 12% of the total mass to obtain a concentrated solution;

[0011] Step 4: Cool the concentrated solution to 60°C, adjust the pH to 5 - 5.5 with citric acid, then centrifuge at a speed of 10000r / min - 12000r / min for 25min - 35min, and then filter with an ultrafilter to obtain a plant extract for skin whitening and anti - aging.

[0012] The main components of Salvia miltiorrhiza involved in the present invention are tanshinone II, tanshinone III, cryptotanshinone, dihydrotanshinone I, protocatechualdehyde, protocatechuic acid, danshensu, vitamin E, etc., and have the following effects: it can dilate the coronary artery, increase coronary blood flow, and improve myocardial ischemia; it can dilate peripheral blood vessels, improve blood circulation, anticoagulate, promote fibrinolysis, inhibit platelet aggregation, and inhibit thrombus formation; it can promote liver cell regeneration and has a fibrotic effect; it can improve the body's hypoxia tolerance; it can promote tissue repair, accelerate fracture healing, shorten the recovery period of red blood cells and hemoglobin, and increase reticulocytes; it has an inhibitory effect on a variety of bacteria and Mycobacterium tuberculosis; it can inhibit the central nervous system and has sedative, analgesic, and tranquilizing effects; it has effects such as enhancing immunity, reducing blood lipid, reducing blood sugar, reducing cholesterol, and anti - tumor.

[0013] Angelica sinensis involved in the present invention contains volatile oil. The main components in the oil are ligustilide, angelicone, carvacrol, etc., and also contain components such as ferulic acid, succinic acid, uracil, amino acids, vitamins, and various inorganic elements essential for the human body, and have the following effects: it can inhibit and excite uterine smooth muscle and has a two - way regulatory effect; it has the effects of anti - platelet aggregation and anti - thrombus formation; it can promote the production of hemoglobin and red blood cells and promote bone marrow hematopoietic function; it has a significant effect of dilating the coronary artery, anti - myocardial ischemia, anti - arrhythmia, and dilating blood vessels; it can enhance the body's immune function, promote liver cell regeneration, protect the liver, and restore liver function; it can antibacterial and inhibit the growth of tumor cells; ligustilide has an anti - asthmatic effect; it has effects such as sedative analgesia, anti - inflammation, anti - hypoxia, and anti - radiation damage.

[0014] The Atractylodes macrocephala involved in the present invention contains volatile oil, and the main components in the oil are atractylol, atractylone, atractylenolide, etc. It also contains various amino acids, polysaccharides, alkynes, vitamin A and other components, and has the following effects: It has effects such as strengthening the body, diuresis, hypoglycemic, anticoagulant, vasodilation, blood pressure lowering, etc.; it can protect the liver, prevent the reduction of glycogen caused by carbon tetrachloride, and the polysaccharides contained can enhance the immune function; the volatile oil has an anti-cancer effect.

[0015] The Dioscorea opposita involved in the present invention contains diosgenin, saponins, mucilage, choline, starch, polysaccharides, glycoproteins, mannan, free amino acids, vitamin C and other components, and has the following effects: It has effects such as nourishing and helping digestion; it can relieve cough and reduce phlegm; it has hypoglycemic and androgen-like enhancing effects; the polysaccharides contained can enhance the immune function and have an anti-aging effect.

[0016] The Rehmannia glutinosa involved in the present invention contains catalpol, rehmannin, mannitol, vitamin A substances, various sugars, amino acids and various inorganic elements and other components, and has the following effects: It can strengthen the heart, diuresis, hypoglycemic, blood pressure lowering; it can enhance the immune function; it can prevent the atrophy of the adrenal cortex and promote the synthesis of adrenal cortical hormones; it has effects such as anti-aging, anti-hyperthyroidism, increasing peripheral white blood cells and antibacterial.

[0017] The Morus alba root bark involved in the present invention mainly contains various flavonoid derivatives, such as mulberrin, morachromene, umbelliferone, scopoletin, morafurane A, and also contains volatile oil, mucilage, pectin, sugars, etc., and has the following effects: It has a blood pressure lowering effect; it has a diuretic effect; it has sedative, tranquilizing, anti-convulsive, analgesic and temperature lowering effects on the nervous system; it has an inhibitory effect on Staphylococcus aureus, Escherichia coli, and Shigella dysenteriae.

[0018] The Pueraria lobata involved in the present invention contains flavonoid substances, mainly daidzein, puerarin, etc., and also contains a large amount of starch and other components, and has the following effects: It can dilate coronary vessels and cerebral vessels, increase coronary blood flow, and reduce myocardial oxygen consumption; it can dilate peripheral vessels, lower blood pressure, relieve the "tight neck" symptom of patients, and has a relaxing effect on gastrointestinal smooth muscle; it has a wide range of β-receptor blocking effects; it has antipyretic and hypoglycemic effects.

[0019] The Coptis chinensis involved in the present invention contains various alkaloid components such as berberine (berberine hydrochloride), coptisine, and methylcoptisine, and has the following effects: It has a broad-spectrum antibacterial effect and has a significant inhibitory effect on Bacillus dysenteriae, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, Corynebacterium diphtheriae, Bordetella pertussis, Mycobacterium tuberculosis, Staphylococcus aureus, Neisseria meningitidis, Hemolytic streptococcus, Streptococcus pneumoniae, etc.; it also has an inhibitory effect on Leptospira, Entamoeba histolytica, Trichomonas, influenza virus, and various pathogenic dermatophytes; when the dose of berberine is small, it can excite the heart, enhance its contractility, and increase the coronary blood flow, while when the dose is large, it inhibits the heart and weakens its contractility; it has antihypertensive, cholagogic, antipyretic, sedative, analgesic, antidiuretic, and local anesthetic effects; it can relax vascular smooth muscle and excite the smooth muscle of the uterus, bladder, and gastrointestinal tract; it has an anticancer effect.

[0020] The Scutellaria baicalensis involved in the present invention contains components such as baicalin, wogonoside, baicalein, and wogonin, and has the following effects: It has a broad-spectrum antibacterial effect and has an inhibitory effect on Salmonella typhi, Bacillus dysenteriae, Bordetella pertussis, Staphylococcus aureus, Streptococcus, Streptococcus pneumoniae, Neisseria meningitidis, etc.; it also has an inhibitory effect on influenza virus, Leptospira, and various pathogenic fungi; it has antipyretic and sedative, antihypertensive, diuretic, cholagogic, hepatoprotective, as well as antiplatelet aggregation, anticoagulation, antioxidant, anti-inflammatory, and anti-allergic reaction effects; it has the effect of relieving allergic asthma; it has a relaxing effect on the smooth muscle of the intestinal tract; it has an antitumor effect.

[0021] The Phellodendron amurense involved in the present invention contains components such as berberine, phellodendrine, obacunone, and phellodendrolactone, and has the following effects: It has an antibacterial effect, and its antibacterial spectrum and antibacterial efficacy are similar to those of Coptis chinensis; it has cholagogic, diuretic antihypertensive, antipyretic, etc. effects, but the efficacy is less than that of Coptis chinensis; it has a hypoglycemic and platelet-protecting effect.

[0022] In some of these embodiments, the skin whitening and anti-aging plant extract is composed of the following raw materials by mass:

[0023] Salvia miltiorrhiza 10 parts - 20 parts, Angelica sinensis 10 parts - 20 parts, Atractylodes macrocephala 10 parts - 20 parts, Dioscorea opposita 10 parts - 20 parts, Rehmannia glutinosa 10 parts - 20 parts, Morus alba root bark 2 parts - 5 parts, Pueraria lobata 2 parts - 5 parts, Coptis chinensis 2 parts - 5 parts, Scutellaria baicalensis 2 parts - 5 parts, Phellodendron amurense 2 parts - 5 parts.

[0024] In some of these embodiments, the skin whitening and anti-aging plant extract is composed of the following raw materials by mass:

[0025] Salvia miltiorrhiza 15 parts, Angelica sinensis 15 parts, Atractylodes macrocephala 15 parts, Dioscorea opposita 15 parts, Rehmannia glutinosa 15 parts, Morus alba root bark 2 parts, Pueraria lobata 2 parts, Coptis chinensis 2 parts, Scutellaria baicalensis 2 parts, Phellodendron amurense 2 parts.

[0026] In some of these embodiments, the skin whitening and anti-aging plant extract is composed of the following raw materials by mass:

[0027] 15 parts of Salvia miltiorrhiza, 15 parts of Angelica sinensis, 10 parts of Atractylodes macrocephala, 10 parts of Dioscorea opposita, 10 parts of Rehmannia glutinosa, 5 parts of Mori cortex, 5 parts of Pueraria lobata, 2 parts of Coptis chinensis, 2 parts of Scutellaria baicalensis, 2 parts of Phellodendron amurense.

[0028] In some of these embodiments, the step of adding a 50% - 90% ethanol aqueous solution to the raw material components and soaking for 12h - 24h in step two specifically includes:

[0029] Adding a 90% ethanol aqueous solution to the raw material components, soaking for 6h - 12h, and then adding a 50% ethanol aqueous solution and continuing to soak for 6h - 12h.

[0030] In some of these embodiments, the mass of water in step two is 25 times the total mass parts of the raw material components.

[0031] In some of these embodiments, the stirring temperature in step two is 90°C and the stirring time is 80 min.

[0032] In some of these embodiments, the concentration of the concentrated solution in step three is 10%.

[0033] In some of these embodiments, the centrifugation speed in step four is 11000 min⁻¹ and the centrifugation time is 30 min.

[0034] In a second aspect, the present invention also provides an application of the skin - whitening and anti - aging plant extract as described in the first aspect in cosmetics, and the cosmetics include facial masks, creams, essences, lotions, mud masks or facial cleansers.

[0035] Compared with the prior art, the present invention has at least the following beneficial effects:

[0036] Based on the traditional Chinese medicine theory of yin - yang and five - elements, the present invention combines Salvia miltiorrhiza, Angelica sinensis, Atractylodes macrocephala, Dioscorea opposita, Rehmannia glutinosa, Mori cortex, Pueraria lobata, Coptis chinensis, Scutellaria baicalensis and Phellodendron amurense to form a skin - whitening and anti - aging plant extract. Salvia miltiorrhiza and Coptis chinensis enter the heart, promoting blood circulation to remove blood stasis and clearing heart fire; Angelica sinensis and Scutellaria baicalensis enter the liver, generating blood and nourishing the blood, clearing liver fire and damp - heat; Mori cortex and Dioscorea opposita enter the lung, nourishing nutrient qi and defensive qi, reaching the skin together; Atractylodes macrocephala and Pueraria lobata enter the spleen, clearing heat and drying dampness, nourishing the skin of the acquired constitution; Rehmannia glutinosa and Phellodendron amurense enter the kidney, nourishing the congenital water source and guiding fire back to its origin. Each effective traditional Chinese medicine ingredient of the plant extract of the present invention is absorbed by the skin, enters the heart, liver, spleen, lung and kidney, regulates the balance of yin and yang, can take into account skin whitening and anti - aging; does not require precious materials, reducing the raw material cost; except for using a small amount of strong traditional Chinese medicines (Phellodendron amurense and Coptis chinensis), the others are all traditional Chinese medicines with a neutral or warm nature, making the efficacy of the plant extract mild.

[0037] The preparation method of the present invention is simple to operate, does not require special equipment, can effectively extract the active ingredients of traditional Chinese medicines, and enables the active ingredients of traditional Chinese medicines to interact and cooperate with each other, taking into account skin whitening and anti - aging. Detailed Embodiments

[0038] To make the above objects, features, and advantages of the present invention more apparent and understandable, the following provides a detailed description of the specific embodiments of the present invention. Many specific details are set forth in the following description to facilitate a thorough understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the spirit of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0039] As used herein, the term "prepared from" is synonymous with "comprising". The terms "comprising", "including", "having", "containing", or any other variation thereof used herein are intended to cover non-exclusive inclusion. For example, a composition, step, method, article, or apparatus containing the listed elements is not necessarily limited to those elements, but may include other elements not expressly listed or elements inherent to such composition, step, method, article, or apparatus.

[0040] When an equivalent, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper preferred values and lower preferred values, it should be understood that all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value are specifically disclosed, regardless of whether the ranges are separately disclosed. For example, when the range "1 to 5" is disclosed, the described range should be interpreted to include ranges "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described herein, unless otherwise stated, the range is intended to include its end values and all integers and fractions within the range.

[0041] Furthermore, the indefinite articles "a" and "an" preceding elements or components of the present invention do not limit the quantity requirement (i.e., the number of occurrences) of the elements or components. Therefore, "a" or "an" should be interpreted to include one or at least one, and the singular form of an element or component also includes the plural form, unless the quantity clearly refers to the singular form.

[0042] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this invention belongs. The terms used in the description of the present invention herein are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the associated listed items.

[0043] Example 1

[0044] A plant extract for skin whitening and anti-aging is composed of the following raw materials by mass parts:

[0045] 3 parts of Salvia miltiorrhiza, 4 parts of Angelica sinensis, 3 parts of Atractylodes macrocephala, 3 parts of Dioscorea opposita, 4 parts of Rehmannia glutinosa, 3 parts of Mori cortex, 2 parts of Pueraria lobata, 1 part of Coptis chinensis, 1 part of Scutellaria baicalensis, 1 part of Phellodendron amurense;

[0046] The preparation method of the skin whitening and anti-aging plant extract comprises the following steps:

[0047] Step 1, weighing each raw material component by mass parts, crushing to 40 mesh, and washing;

[0048] Step 2, adding 90% ethanol aqueous solution to the raw material components, infiltrating for 6 h, then adding 50% ethanol aqueous solution with the same mass as the 90% ethanol aqueous solution, continuously infiltrating for 12 h, adding 25 times the total mass parts of water of the raw material components, heating to 90 °C while stirring, keeping warm and stirring for 80 min, and then filtering with an ultrafilter to obtain a filtrate;

[0049] Step 3, performing mixed evaporation and concentration on the filtrate until the concentration reaches 10% of the total mass parts to obtain a concentrated solution;

[0050] Step 4, cooling the concentrated solution to 60 °C, adjusting the pH to 5 - 5.5 with citric acid, then centrifuging at 11000 r / min for 30 min, and filtering with an ultrafilter to obtain the skin whitening and anti-aging plant extract.

[0051] Example 2

[0052] A skin whitening and anti-aging plant extract is composed of the following raw materials by mass parts:

[0053] 10 parts of Salvia miltiorrhiza, 10 parts of Angelica sinensis, 10 parts of Atractylodes macrocephala, 10 parts of Dioscorea opposita, 10 parts of Rehmannia glutinosa, 2 parts of Mori cortex, 2 parts of Pueraria lobata, 2 parts of Coptis chinensis, 2 parts of Scutellaria baicalensis, 2 parts of Phellodendron amurense;

[0054] The preparation method of the skin whitening and anti-aging plant extract comprises the following steps:

[0055] Step 1, weighing each raw material component by mass parts, crushing to 60 mesh, and washing;

[0056] Step 2, adding 90% ethanol aqueous solution to the raw material components, infiltrating for 6 h, then adding 50% ethanol aqueous solution with the same mass as the 90% ethanol aqueous solution, continuously infiltrating for 6 h, adding 20 times the total mass parts of water of the raw material components, heating to 88 °C while stirring, keeping warm and stirring for 80 min, and then filtering with an ultrafilter to obtain a filtrate;

[0057] Step 3, performing mixed evaporation and concentration on the filtrate until the concentration reaches 10% of the total mass parts to obtain a concentrated solution;

[0058] Step 4: Cool the concentrated solution to 60°C, adjust the pH to 5 - 5.5 with citric acid, then centrifuge at 10,000 r / min for 35 min, and filter with an ultrafilter to obtain the skin-whitening and anti-aging plant extract.

[0059] Example 3

[0060] A skin-whitening and anti-aging plant extract is composed of the following raw materials in parts by mass:

[0061] Salvia miltiorrhiza 15 parts, Angelica sinensis 15 parts, Atractylodes macrocephala 10 parts, Dioscorea opposita 10 parts, Rehmannia glutinosa 10 parts, Morus alba root bark 5 parts, Pueraria lobata 5 parts, Coptis chinensis 2 parts, Scutellaria baicalensis 2 parts, Phellodendron amurense 2 parts;

[0062] The preparation method of the skin-whitening and anti-aging plant extract is the same as that in Example 1.

[0063] Example 4

[0064] A skin-whitening and anti-aging plant extract is composed of the following raw materials in parts by mass:

[0065] Salvia miltiorrhiza 15 parts, Angelica sinensis 15 parts, Atractylodes macrocephala 15 parts, Dioscorea opposita 15 parts, Rehmannia glutinosa 15 parts, Morus alba root bark 2 parts, Pueraria lobata 2 parts, Coptis chinensis 2 parts, Scutellaria baicalensis 2 parts, Phellodendron amurense 2 parts;

[0066] The preparation method of the skin-whitening and anti-aging plant extract is the same as that in Example 1.

[0067] Example 5

[0068] The skin-whitening and anti-aging plant extract is composed of the following raw materials in parts by mass:

[0069] Salvia miltiorrhiza 20 parts, Angelica sinensis 20 parts, Atractylodes macrocephala 20 parts, Dioscorea opposita 20 parts, Rehmannia glutinosa 20 parts, Morus alba root bark 5 parts, Pueraria lobata 5 parts, Coptis chinensis 5 parts, Scutellaria baicalensis 5 parts, Phellodendron amurense 5 parts;

[0070] The preparation method of the skin-whitening and anti-aging plant extract includes the following steps:

[0071] Step 1: Weigh each raw material component according to the parts by mass, crush to 20 mesh, and wash.

[0072] Step 2: Add 90% ethanol aqueous solution to the raw material components, soak for 12 h, then add 50% ethanol aqueous solution with the same mass as the 90% ethanol aqueous solution, continue to soak for 12 h, add 30 times the total mass parts of water to the raw material components, heat to 95°C while stirring, keep stirring for 70 min, and then filter the filtrate with an ultrafilter.

[0073] Step 3: Mix and evaporate and concentrate the filtrate until the concentration reaches 10% of the total mass parts to obtain the concentrated solution.

[0074] Step 4: Cool the concentrated liquid to 60°C, adjust the pH to 5 - 5.5 with citric acid, then centrifuge at 12,000 r / min for 25 min, and filter with an ultrafilter to obtain the skin-whitening and anti-aging plant extract.

[0075] Comparative Example 1

[0076] The difference from Example 3 is that Comparative Example 1 does not contain Salvia miltiorrhiza and Coptis chinensis.

[0077] Comparative Example 2

[0078] The difference from Example 3 is that Comparative Example 2 does not contain Angelica sinensis and Scutellaria baicalensis.

[0079] Comparative Example 3

[0080] The difference from Example 3 is that Comparative Example 3 does not contain Mori Cortex and Dioscorea opposita.

[0081] Comparative Example 4

[0082] The difference from Example 3 is that Comparative Example 4 does not contain Atractylodes macrocephala and Pueraria lobata.

[0083] Comparative Example 5

[0084] The difference from Example 3 is that Comparative Example 5 does not contain Rehmannia glutinosa and Phellodendron amurense.

[0085] Comparative Example 6

[0086] The difference from Example 3 is that Comparative Example 6 also contains 15 parts of Ligusticum chuanxiong.

[0087] (I) Whitening efficacy test of plant extract

[0088] Test samples: The skin-whitening and anti-aging plant extracts prepared in Examples 3 and 4, and arbutin was used in the control group.

[0089] Test method: Using 1 mL of 0.5 mmol / L L-tyrosine as the substrate, add the test sample, phosphate buffer solution (PBS) with pH = 6.8, and 0.1% (mass fraction) dopa solution according to Table 1 below, mix well, and keep it at a constant temperature in a water bath at 37°C; then add 110 U / mL of tyrosinase solution, incubate at 37°C for 10 min, quickly transfer it to a cuvette, and measure the absorbance at 475 nm. Tyrosinase inhibition rate: I = [(A1 - A2) - (A3 - A4)] / (A1 - A2) × 100%; A1, A2, A3, and A4 are the absorbances of Group 1 - Group 4 respectively. The test results are shown in Table 2.

[0090] Table 1 Composition of reaction solution

[0091] Reaction group Sample solution / mL PBS solution / mL Dopa solution / mL Tyrosinase / mL Group 1 0 2 1 0.5 Group 2 0 2.5 1 0 Group 3 1 1 1 0.5 Group 4 1 1.5 1 0

[0092] Table 2 Tyrosinase inhibition rate

[0093] Example 3 Example 4 Arbutin Tyrosinase inhibition rate 93.4％ 90.8％ 56.9％

[0094] As can be seen from Table 2, the plant extracts prepared in Examples 3 and 4 have an inhibitory effect on tyrosinase and have a more significant whitening effect compared to arbutin.

[0095] (II) Anti-aging efficacy test of plant extracts

[0096] Test samples: Skin whitening and anti-aging plant extracts prepared in Examples 3 and 4, and vitamin C was used as the control group.

[0097] Test method: Add 3.0 mL of 1×10 -4 mol / L DPPH solution and 0.5 mL of sample solution into a test tube in sequence, mix well, react in the dark for 30 min, measure the absorbance at 517 nm, and measure in parallel 3 times, denoted as As; add 3.0 mL of DPPH solution and 0.5 mL of sample dilution solvent into a test tube, mix well, and measure the absorbance value, denoted as Ao; add 3.0 mL of DPPH solution dilution solvent and 0.5 mL of sample solution into a test tube, mix well, and measure the absorbance value, denoted as Ar. DPPH radical scavenging rate: Y = [1 - (As - Ar) / Ao] × 100%. The test results are shown in Table 3.

[0098] Table 3 DPPH radical scavenging rate

[0099] Example 3 Example 4 Vitamin C DPPH scavenging rate 96.4％ 95.7％ 95.0％

[0100] As can be seen from Table 3, the ability of the plant extracts prepared in Examples 3 and 4 to scavenge DPPH radicals is equivalent to that of vitamin C, and they have the effect of anti-wrinkle.

[0101] (III) Human trial evaluation

[0102] Test samples: Sheet masks prepared from Examples 1 to 5 and Comparative Examples 1 to 6.

[0103] Test subjects: 55 female volunteers aged 35 to 50 years old, and 5 people were assigned to try each test sample.

[0104] Test steps: The subjects cleaned their faces with a commercially available amino acid facial cleanser product, dried their skin with a facial towel, and sat still in a waiting room at a temperature of 25 ± 1°C and a humidity of 50 ± 10% RH for more than 30 minutes; took facial images of the subjects, and used a glossiness probe to measure the basic facial glossiness value of the subjects. The subjects used the facial mask once a day with the same process, and the subjects evaluated whether allergic reactions occurred each time they used it. Took facial images once a day and measured the facial glossiness once a day. According to the facial glossiness, counted the number of days required to brighten the skin tone (skin tone improvement rate greater than 40%); according to the facial images, counted the number of days required for the spot color to fade (spot color reduction rate greater than 30%) and the number of days required for wrinkle reduction (wrinkle reduction rate greater than 30%).

[0105] Test results:

[0106] Example 1: Occasionally, the subjects had slight facial fever during the use process, and the discomfort of the heat sensation disappeared within 5 minutes after application, which belonged to normal drug absorption; the skin tone was brightened within an average of 8 days, the spot color became lighter within an average of 23 days, wrinkles were reduced within an average of 37 days, and the facial relaxation phenomenon was improved.

[0107] Example 2: There were no allergic reactions such as redness, itching, and swelling during the use process; the skin tone was brightened within an average of 9 days, the spot color became lighter within an average of 21 days, wrinkles were reduced within an average of 35 days, and the facial relaxation phenomenon was improved.

[0108] Example 3: There were no allergic reactions such as redness, itching, and swelling during the use process; the skin tone was brightened within an average of 6 days, the spot color became lighter within an average of 13 days, wrinkles were reduced within an average of 30 days, and the facial relaxation phenomenon was improved.

[0109] Example 4: There were no allergic reactions such as redness, itching, and swelling during the use process; the skin tone was brightened within an average of 7 days, the spot color became lighter within an average of 18 days, wrinkles were reduced within an average of 28 days, and the facial relaxation phenomenon was improved

[0110] Example 5: Occasionally, the subjects had slight facial fever during the use process, and the discomfort of the heat sensation disappeared within 5 minutes after application, which belonged to normal drug absorption; the skin tone was brightened within an average of 8 days, the spot color became lighter within an average of 22 days, wrinkles were reduced within an average of 36 days, and the facial relaxation phenomenon was improved.

[0111] Comparative Example 1: There were no allergic reactions such as redness, itching, and swelling during the use process; the skin tone was brightened within an average of 18 days, the spot color became lighter within an average of 32 days, wrinkles were reduced within an average of 60 days, and the facial relaxation phenomenon was improved.

[0112] Comparative Example 2: There were no allergic reactions such as redness, itching, and swelling during the use process; the skin tone was brightened within an average of 15 days, the spot color became lighter within an average of 30 days, wrinkles were reduced within an average of 65 days, and the facial relaxation phenomenon was improved.

[0113] Comparative Example 3: Occasionally, the face of the subject felt slightly warm during use, and the discomfort caused by the heat disappeared within 20 minutes after application, which was normal drug absorption; the skin tone was brightened within an average of 15 days, the color of the spots became lighter within an average of 28 days, the wrinkles were reduced within an average of 55 days, and the phenomenon of facial relaxation was improved.

[0114] Comparative Example 4: Occasionally, the face of the subject felt slightly warm during use, and the discomfort caused by the heat disappeared within 20 minutes after application, which was normal drug absorption; the skin tone was brightened within an average of 18 days, the color of the spots became lighter within an average of 33 days, the wrinkles were reduced within an average of 65 days, and the phenomenon of facial relaxation was improved.

[0115] Comparative Example 5: There was no allergic reaction such as redness, itching, or swelling during use; the skin tone was brightened within an average of 16 days, the color of the spots became lighter within an average of 31 days, the wrinkles were reduced within an average of 60 days, and the phenomenon of facial relaxation was improved.

[0116] Comparative Example 6: There was no allergic reaction such as redness, itching, or swelling during use; the skin tone was not brightened in 10 days, the color of the spots did not become lighter in 22 days, and the wrinkles were not reduced in 35 days.

[0117] It can be seen that, compared with Comparative Examples 1 to 5, Examples 1 to 5 can all significantly whiten and resist aging; compared with Comparative Example 6, it was unexpectedly found that the whitening effect and anti-aging effect when adding Ligusticum chuanxiong on the basis of Example 3 were inferior to those without adding Ligusticum chuanxiong. The compounding of Salvia miltiorrhiza, Angelica sinensis, Atractylodes macrocephala, Dioscorea opposita, Rehmannia glutinosa, Morus alba root bark, Pueraria lobata, Coptis chinensis, Scutellaria baicalensis, and Phellodendron amurense in the present invention can play a synergistic effect and has excellent effects of brightening the skin tone, fading spots, anti-aging, and anti-wrinkle.

[0118] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered to be within the scope described in this specification.

[0119] The above-described specific embodiments have further detailed the purpose, technical solutions, and beneficial effects of the present invention. It should be understood that the above are only specific embodiments of the present invention and are not used to limit the protection scope of the present invention. In particular, it is pointed out that for those skilled in the art, any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A skin whitening and anti-aging plant extract, characterized in that: It is composed of the following raw materials in parts by mass: 3-30 parts of Danshen, 4-40 parts of Angelica, 3-30 parts of Atractylodes, 3-30 parts of Dioscorea, 4-40 parts of Rehmannia, 3-30 parts of Morus alba, 2-20 parts of Pueraria, 1-10 parts of Coptis, 1-10 parts of Scutellaria, 1-10 parts of Phellodendron; The preparation method of the skin whitening and anti-aging plant extract comprises the following steps: Step 1, weigh each raw material component by mass, crush it into 20-60 mesh, and wash it; Step 2: Add 50% to 90% ethanol aqueous solution to the raw material components, soak for 12 hours to 24 hours, add 20 to 30 times the total weight of the raw material components of water, heat to 88°C to 95°C while stirring, keep warm and stir for 70 minutes to 80 minutes, and then filter the filtrate with an ultrafilter; Step 3, mixing and evaporating the filtrate until the concentration reaches 8% to 12% of the total mass to obtain a concentrated solution; Step 4, cooling the concentrated solution to 60° C., adjusting the pH to 5-5.5 with citric acid, centrifuging at 10000 r / min-12000 r / min for 25 min-35 min, and filtering with an ultrafilter to obtain a skin whitening and anti-aging plant extract.

2. The skin whitening and anti-aging plant extract according to claim 1, characterized in that: It is composed of the following raw materials in parts by mass: 10-20 parts of Salvia miltiorrhiza, 10-20 parts of Angelica sinensis, 10-20 parts of Atractylodes macrocephala, 10-20 parts of Dioscorea opposita, 10-20 parts of Rehmannia glutinosa, 2-5 parts of Morus alba bark, 2-5 parts of Pueraria root, 2-5 parts of Coptis chinensis, 2-5 parts of Scutellaria baicalensis, and 2-5 parts of Phellodendron chinense.

3. The skin whitening and anti-aging plant extract according to claim 2, characterized in that: It is composed of the following raw materials in parts by mass: 15 parts of Salvia miltiorrhiza, 15 parts of Angelica sinensis, 15 parts of Atractylodes macrocephala, 15 parts of Dioscorea opposita, 15 parts of Rehmannia glutinosa, 2 parts of Morus alba bark, 2 parts of Pueraria root, 2 parts of Coptis chinensis, 2 parts of Scutellaria baicalensis, and 2 parts of Phellodendron chinense.

4. The skin whitening and anti-aging plant extract according to claim 2, characterized in that: It is composed of the following raw materials in parts by mass: 15 parts of Salvia miltiorrhiza, 15 parts of Angelica sinensis, 10 parts of Atractylodes macrocephala, 10 parts of Dioscorea opposita, 10 parts of Rehmannia glutinosa, 5 parts of Morus alba, 5 parts of Pueraria root, 2 parts of Coptidis rhizome, 2 parts of Scutellaria baicalensis, and 2 parts of Phellodendron chinense.

5. The skin whitening and anti-aging plant extract according to claim 1, characterized in that: The step of adding 50% to 90% ethanol aqueous solution to the raw material components and soaking for 12h to 24h in step 2 specifically includes: Add 90% ethanol aqueous solution to the raw material components, soak for 6h to 12h, then add 50% ethanol aqueous solution, and continue soaking for 6h to 12h.

6. The skin whitening and anti-aging plant extract according to claim 1, characterized in that: The mass of water in step 2 is 25 times the total mass of the raw material components.

7. The skin whitening and anti-aging plant extract according to claim 1, characterized in that: In the step 2, the stirring temperature is 90° C. and the stirring time is 80 min.

8. The skin whitening and anti-aging plant extract according to claim 1, characterized in that: The concentration of the concentrated solution in step 3 is 10%.

9. The skin whitening and anti-aging plant extract according to claim 1, characterized in that: In the step 4, the centrifugal speed is 11000 r / min and the centrifugal time is 30 min.

10. Use of the skin whitening and anti-aging plant extract according to any one of claims 1 to 9 in the preparation of cosmetics, characterized in that: The cosmetics include facial masks, facial creams, essences or lotions.