A Chinese medicine composition for improving muscle fat infiltration and its preparation method and application

Abstract

The present invention relates to the technical field of traditional Chinese medicine, and more particularly to a Chinese medicine composition for improving muscle fat infiltration, and a preparation method and application thereof. The Chinese medicine composition for improving muscle fat infiltration of the present invention comprises the following raw materials in parts by weight: 10-30g of Rehmannia glutinosa, 10-30g of Psoralea corylifolia, 6-20g of Cistanche deserticola, 6-20g of Eucommia ulmoides, 6-20g of Ginseng, 6-20g of Astragalus, 6-20g of Poria, 6-20g of Atractylodes macrocephala, 6-20g of Lycium barbarum, 6-20g of Angelica sinensis, 6-20g of Carthamus tinctorius, 6-20g of Salvia miltiorrhiza, 6-20g of Cyathula officinalis, and 3-10g of Licorice. The Chinese medicine composition formula has the effect of reducing muscle fat content, and has good safety, can be applied to the clinical treatment of improving muscle fat infiltration, and has broad application prospects.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and in particular to a traditional Chinese medicine composition for improving muscle fat infiltration, and a preparation method and application thereof. Background Art

[0002] As we age, ectopic adipose tissue will infiltrate skeletal muscle, leading to increased intramuscular fat content, which in turn causes muscle strength and function to decline. Increased intramuscular fat content is closely related to many metabolic diseases (such as diabetes and obesity). The fat content in skeletal muscle is significantly increased in diseases such as stroke, ischemic muscle contracture, and Duchenne muscular atrophy, and some musculoskeletal diseases (such as knee osteoarthritis, rotator cuff injury, etc.) are often accompanied by skeletal muscle fat infiltration. At the same time, short-term limb disuse also significantly increases the fat content in the muscle. Due to reduced mobility, fat deposits in the limbs and paraspinal muscles of the elderly, and the fat content is significantly higher than that of young people. However, abnormal intramuscular adipose tissue and lipid metabolism will cause inflammation, and increased muscle fat content will lead to weakened muscle strength and function, further affecting joint stability and functional activities, showing a decrease in body mobility, increasing the risk of falls, fractures, and even death. In addition, intramuscular fat can also affect cartilage and bone metabolism through "muscle-bone" coupling, thereby leading to a series of musculoskeletal diseases, such as knee osteoarthritis and osteoporosis, which seriously endanger people's health.

[0003] Fat infiltration in muscles can lead to weakened muscle function, decreased body mobility, bone and joint diseases, etc., which can lead to limb disuse and further aggravate fat deposition in muscles, forming a vicious cycle. At present, the methods to reduce muscle fat content mainly include exercise therapy and nutritional support, but resistance exercise is difficult to apply clinically for older people with decreased motor ability, and compliance is poor; it is difficult to reduce muscle fat content with nutritional supplementation alone, and nutritional support has the risk of aggravating the condition of patients with other chronic diseases. For example, excessive protein supplementation will increase the burden on the kidneys and cause renal dysfunction. At present, there is no specific drug to reduce intramuscular fat content. Summary of the invention

[0004] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a Chinese medicine composition for improving muscle fat infiltration, which is a kidney-tonifying, spleen-strengthening and blood-activating prescription. It improves muscle quality by reducing muscle fat infiltration, and provides a new treatment method for the prevention, treatment and rehabilitation of musculoskeletal diseases.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] In a first aspect, the present invention provides a traditional Chinese medicine composition for improving muscle fat infiltration, comprising the following raw materials in parts by weight: 10-30 parts of cooked rehmannia, 10-30 parts of psoralea corylifolia, 6-20 parts of Cistanche deserticola, 6-20 parts of Eucommia ulmoides, 6-20 parts of ginseng, 6-20 parts of astragalus, 6-20 parts of Poria, 6-20 parts of Atractylodes macrocephala, 6-20 parts of wolfberry, 6-20 parts of angelica, 6-20 parts of safflower, 6-20 parts of salvia miltiorrhiza, 6-20 parts of Cyathula obtusifolia, and 3-10 parts of licorice.

[0007] Traditional Chinese medicine believes that the kidney stores essence and transforms it into qi, which controls the growth and development of the body; the spleen controls muscles and limbs. The innate essence and the acquired essence transformed by the spleen and stomach are stored in the kidney, and the kidney essence nourishes the spleen. The two are interdependent and mutually useful to maintain the normal function of the muscles. If the kidney essence is insufficient, it cannot nourish the tendons, bones and muscles, which can cause the bones and flesh to be out of touch, and the muscles become weak and soft; if the spleen is deficient, the muscles will have no qi and blood to irrigate, and will be atrophied and weak due to lack of nutrition, and fat will be deposited. At the same time, the kidney is the innate foundation, and the spleen is the acquired foundation. The kidney stores essence and needs spleen essence to supplement it. If the innate kidney qi cannot be nourished by the acquired qi and blood, it will develop into kidney deficiency; the spleen controls transportation and transformation, and depends on the warmth of life fire. If the spleen is not nourished by kidney essence, the spleen qi will be deficient and transportation and transformation will be weak. It can be seen that the spleen and kidney can aggravate the deterioration of muscle quality through mutual influence. In addition, blood stasis blocks the collaterals, qi and blood cannot reach the muscles normally, or the collaterals are weak, and blood stays in the muscles and turns into blood stasis, resulting in muscle malnutrition and muscle atrophy. Therefore, "deficiency of both spleen and kidney and blood stasis" is the root cause of muscle atrophy, and blood stasis is its important pathogenic factor.

[0008] The present invention aims at spleen and kidney deficiency as the key pathogenesis of muscular atrophy, and uses Rehmannia glutinosa and Psoralea corylifolia as the main medicine. Psoralea corylifolia can nourish the kidney and assist yang, and can "warm the water and soil, digest food, and reach the spleen and stomach"; Rehmannia glutinosa can nourish kidney yin, and "Compendium of Materia Medica" "fills bone marrow and grows muscles"; Psoralea corylifolia belongs to the spleen and kidney meridians, and Rehmannia glutinosa belongs to the kidney meridian and is the main medicine, nourishing yin and yang, nourishing the kidney and strengthening the spleen, warming the soil and strengthening the muscles. Cistanche deserticola, Eucommia ulmoides, Polygonatum sibiricum, Ginseng, Astragalus, Poria cocos and Atractylodes macrocephala are used as the main medicines, and they have the effects of strengthening the kidney and essence, and invigorating qi and strengthening the spleen. With Angelica sinensis, Carthamus tinctorius, Salvia miltiorrhiza, and Cyathula obtusifolia, blood circulation and blood stasis are promoted, meridians are warmed and collaterals are dredged, and the remaining troubles due to deficiency and arthralgia are eliminated; Cyathula obtusifolia can nourish the liver and kidney, strengthen the waist and knees, and can lead blood downward. Licorice is used as a messenger to reconcile the medicines. The combination of drugs complements each other and achieves the effects of nourishing the kidney, strengthening the spleen and activating blood circulation, can reduce intramuscular fat content and enhance muscle strength. The weight portion of the Chinese medicine composition is within the scope of the present invention and is used to improve muscle quality with good efficacy.

[0009] Preferably, the Chinese medicine composition for improving muscle fat infiltration comprises the following components in parts by weight: 15 parts of Rehmannia glutinosa, 15 parts of Psoralea corylifolia, 10 parts of Cistanche deserticola, 10 parts of Eucommia ulmoides, 10 parts of ginseng, 10 parts of Astragalus, 10 parts of Poria, 10 parts of Atractylodes macrocephala, 10 parts of Lycium barbarum, 10 parts of Angelica sinensis, 10 parts of Safflower, 10 parts of Salvia miltiorrhiza, 10 parts of Cyathula capitata, and 5 parts of Licorice.

[0010] After a large number of experimental explorations, the present invention found that the Chinese medicine composition for improving muscle fat infiltration has the best effect of increasing the cross-section of muscle fibers and reducing fat cell infiltration when the Chinese medicine composition is in a specific weight portion of the present invention.

[0011] In a second aspect, the present invention provides a method for preparing the above-mentioned Chinese medicine composition for improving muscle fat infiltration, comprising the following steps:

[0012] (1) weighing the raw materials according to weight, soaking them in water, heating them for decoction, and filtering the filtrate;

[0013] (2) adding water to the filter residue of step (1) for secondary decoction, and filtering to obtain the filtrate;

[0014] (3) The filtrates from step (1) and step (2) are combined and concentrated to obtain the Chinese medicine composition for improving muscle fat infiltration.

[0015] Preferably, in step (1), the amount of water added is 10 times the volume of the prepared raw material.

[0016] Preferably, in step (1), the soaking time is 1 hour.

[0017] Preferably, in step (2), the amount of water added is 5 times the volume of the prepared raw materials.

[0018] Preferably, in steps (1) and (2), the decocting time is 30 minutes.

[0019] In a third aspect, the present invention provides use of the above-mentioned Chinese medicine composition for improving muscle fat infiltration in the preparation of a medicament for improving muscle fat infiltration.

[0020] In a fourth aspect, the present invention provides a drug for improving muscle fat infiltration, including the above-mentioned Chinese medicine composition for improving muscle fat infiltration.

[0021] Preferably, pharmaceutically acceptable excipients are also included.

[0022] The beneficial effects of the present invention are:

[0023] Traditional Chinese medicine believes that "the spleen governs muscles" and "the kidney governs bones", and the spleen and kidney are in charge of muscles and bones. Spleen deficiency can lead to disorders in the transportation and transformation of water and grain essence, which in turn causes the kidney essence to lose the nourishment of acquired qi, and the marrow cannot produce bones. At the same time, the "kidney qi" of middle-aged and elderly people gradually declines with age, and the spleen will be affected due to the "spleen and kidney relationship", forming a vicious circle. At the same time, if both the spleen and kidney are deficient, the qi and blood will not flow, stagnate and leave blood stasis, and the meridians will be blocked and cause arthralgia, further aggravating the condition. Therefore, "spleen and kidney deficiency, blood stasis and stagnation" is the TCM pathogenesis of KOA. The present invention takes the kidney-tonifying, spleen-strengthening and blood-activating method as the treatment principle, and the prepared Chinese medicine composition can effectively improve muscle fat infiltration and improve muscle quality. The Chinese medicine composition has good safety and can be applied to the clinical treatment of improving muscle fat infiltration, and has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 HE staining images of muscles in each group. DETAILED DESCRIPTION

[0025] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0026] In the embodiment of the present invention:

[0027] Chinese medicine (Rehmannia glutinosa, Psoralea corylifolia, Cistanche deserticola, Eucommia ulmoides, Ginseng, Astragalus, Poria, Atractylodes macrocephala, Lycium barbarum, Angelica sinensis, Safflower, Salvia miltiorrhiza, Cyathula officinalis, Licorice) was purchased from the pharmacy of Guangdong Second Hospital of Traditional Chinese Medicine.

[0028] Detection kit: Nanjing Jiancheng Biological.

[0029] Embodiment 1:

[0030] An embodiment of the Chinese medicine composition for improving muscle fat infiltration according to the present invention comprises the following raw materials in parts by weight: 15g of Rehmannia glutinosa, 15g of Psoralea corylifolia, 10g of Cistanche deserticola, 10g of Eucommia ulmoides, 10g of Ginseng, 10g of Astragalus, 10g of Poria, 10g of Atractylodes macrocephala, 10g of Lycium barbarum, 10g of Angelica sinensis, 10g of Safflower, 10g of Salvia miltiorrhiza, 10g of Cyathula capitata, and 5g of Licorice.

[0031] The preparation method of the Chinese medicine composition for improving muscle fat infiltration described in this embodiment comprises the following steps:

[0032] (1) Weigh the raw materials according to their weight, add 10 times the volume of water and soak for 1 hour, boil over high heat, then continue to boil over low heat for 30 minutes, and filter through gauze to obtain the filtrate;

[0033] (2) adding 5 times the volume of water to the residue from step (1), bringing to a boil over high heat and continuing to boil for 30 min, and filtering to obtain the filtrate;

[0034] (3) The filtrates of step (1) and step (2) are mixed and concentrated in a rotary evaporator to obtain the Chinese medicine composition for improving muscle fat infiltration.

[0035] Embodiment 2:

[0036] An embodiment of the Chinese medicine composition for improving muscle fat infiltration according to the present invention comprises the following raw materials in parts by weight: 10g of Rehmannia glutinosa, 10g of Psoralea corylifolia, 20g of Cistanche deserticola, 20g of Eucommia ulmoides, 20g of Ginseng, 20g of Astragalus, 20g of Poria, 20g of Atractylodes macrocephala, 6g of Lycium barbarum, 6g of Angelica sinensis, 20g of Carthamus tinctorius, 6g of Salvia miltiorrhiza, 20g of Cyathula capitata, and 10g of Licorice.

[0037] The preparation method of the Chinese medicine composition for improving muscle fat infiltration described in this example is the same as that in Example 1.

[0038] Embodiment 3:

[0039] An embodiment of the Chinese medicine composition for improving muscle fat infiltration according to the present invention comprises the following raw materials in parts by weight: 30g of Rehmannia glutinosa, 30g of Psoralea corylifolia, 6g of Cistanche deserticola, 6g of Eucommia ulmoides, 6g of Ginseng, 6g of Astragalus, 6g of Poria, 6g of Atractylodes macrocephala, 20g of Lycium barbarum, 20g of Angelica sinensis, 6g of Carthamus tinctorius, 20g of Salvia miltiorrhiza, 6g of Cyathula capitata, and 3g of Licorice.

[0040] The preparation method of the Chinese medicine composition for improving muscle fat infiltration described in this example is the same as that in Example 1.

[0041] Test Example 1:

[0042] Experimental animals: 40 male SD rats, SPF grade, weighing (220±20) g.

[0043] Test sample: the Chinese medicine composition prepared in Example 1.

[0044] Modeling: The assistant holds the rat's neck and back with one hand, and fixes the rat's tail and left hind limb with the other hand. Botulinum toxin A is prepared with physiological saline to 10U / mL, and injected intramuscularly at the midpoint of the right rectus muscle, vastus medialis muscle and vastus lateralis muscle at a dose of 10U / Kg. The model is successfully established after only a single injection and the gait change of the rat after modeling. The model is established for 4 weeks.

[0045] Gavage: The experimental mice were randomly divided into 4 groups, with 10 mice in each group. Gavage time: Gavage started on the 29th day after the injection of botulinum toxin and continued for 28 days;

[0046] (1) Normal dose group of Chinese medicine composition: Based on the body surface area conversion method, the dose is 11.25 g (effective substance mass of the Chinese medicine composition) / Kg.

[0047] (2) High-dose group of Chinese medicine composition: Based on the body surface area conversion method, the dose is 22.5 g (effective amount of the Chinese medicine composition) / Kg.

[0048] (3) Blank group: normal mice, intragastrically administered with an equal volume of saline;

[0049] (4) Model group: intragastric administration of an equal volume of normal saline;

[0050] Sampling: Two hours after the end of intragastric administration on the 56th day, the body weight and limb grasping strength of rats in each group were measured, and then the rats were killed. The quadriceps femoris muscles on both sides of the rats in each group were collected and weighed. The right quadriceps femoris muscle of each rat was cross-sectioned into two parts, one half was sectioned and stained with HE, and the other half was measured with a kit to measure the triglyceride and cholesterol content in the muscle tissue.

[0051] Experimental results:

[0052] 1. Muscle HE staining: From the results of HE staining of the sections, the blank group had a larger cross-sectional area of ​​muscle fibers, and the gaps between muscle fibers were uniform and narrow; the model group had a smaller cross-sectional area of ​​muscle fibers, and the gaps between muscle fibers became wider, with local inflammatory infiltration and a large number of vacuolar fat cells. After the intervention of the Chinese medicine composition, the above pathological phenomena were improved, among which the normal dose group of the Chinese medicine composition had the best effect of increasing the cross-sectional area of ​​muscle fibers and reducing the infiltration of fat cells ( Figure 1 ).

[0053] 2. Intramuscular triglyceride and total cholesterol content:

[0054] 50 mg of quadriceps femoris of rats in each group was weighed and homogenized with physiological saline, and the test was performed according to the instructions of the kit (Nanjing Jiancheng Biological, catalog number: A111-1-1, A110-1-1). The results are shown in Table 1. The contents of total cholesterol and triglyceride in the quadriceps femoris of the model group were higher than those in the blank group (all P<0.05), while the contents of total cholesterol and triglyceride in the quadriceps femoris of the high-dose group and the normal-dose group of the Chinese medicine composition were significantly lower than those in the model group, and the differences were statistically significant (all P<0.05).

[0055] Table 1 Determination of total cholesterol and triglyceride content in each group

[0056] Group Total cholesterol Triglycerides High dose group <![CDATA[0.0063±0.00 **## ]]> <![CDATA[0.0167±0.001 **## ]]> Normal dose group <![CDATA[0.0073±0.00 **## ]]> <![CDATA[0.0180±0.002 **## ]]> Model Group 0.0079±0.00 0.024±0.001 Blank Group <![CDATA[0.0060±0.00 **## ]]> 0.005±0.003

[0057] Note: Compared with the blank group, *P<0.05, **P<0.01; compared with the model group, # P<0.05, ## P<0.01.

[0058] 3. Muscle wet weight ratio: The rats were weighed and the weights of the quadriceps femoris on both sides were weighed. The wet weight ratio and wet weight to body weight ratio were calculated according to formula (1) and formula (2). The results are shown in Table 2.

[0059] Wet weight ratio = (right quadriceps weight / left quadriceps weight) × 100% - Formula (1)

[0060] Wet weight to body weight ratio = (right quadriceps wet weight / body weight) × 100%) - Formula (2)

[0061] Table 2 Statistics of wet weight, wet weight ratio and wet weight to body weight ratio of quadriceps in each group (x±s, mmol / gprot)

[0062]

[0063] Note: Compared with the blank group, * P<0.05, ** P<0.01; compared with the model group, # P<0.05, ## P<0.01.

[0064] 4. The grip strength of the limbs was measured, and the grip strength of the limbs and the grip force-to-weight ratio of each group were counted (the grip strength alone may be affected by different body weights, and the conversion to body weight ratio can eliminate the influence of body weight factors to a certain extent). The results are shown in Table 3.

[0065] Table 3 Statistics of limb grip strength and grip strength to body weight ratio in each group (x±s)

[0066]

[0067]

[0068] Note: Compared with the blank group, * P<0.05, ** P<0.01; compared with the model group, # P<0.05, ## P<0.01.

[0069] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A Chinese medicine composition for improving muscle fat infiltration, characterized in that: The invention is prepared from the following raw materials in parts by weight: 10-30 parts of cooked rehmannia, 10-30 parts of psoralea corylifolia, 6-20 parts of cistanche deserticola, 6-20 parts of eucommia bark, 6-20 parts of ginseng, 6-20 parts of astragalus, 6-20 parts of poria, 6-20 parts of atractylodes macrocephala, 6-20 parts of wolfberry, 6-20 parts of angelica, 6-20 parts of safflower, 6-20 parts of salvia miltiorrhiza, 6-20 parts of cyathula and 3-10 parts of liquorice.

2. The Chinese medicine composition for improving muscle fat infiltration as claimed in claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 15 parts of cooked rehmannia, 15 parts of psoralea corylifolia, 10 parts of cistanche deserticola, 10 parts of eucommia bark, 10 parts of ginseng, 10 parts of astragalus, 10 parts of tuckahoe, 10 parts of atractylodes macrocephala, 10 parts of wolfberry, 10 parts of angelica, 10 parts of safflower, 10 parts of salvia miltiorrhiza, 10 parts of cyathula, and 5 parts of liquorice.

3. A method for preparing a Chinese medicine composition for improving muscle fat infiltration as claimed in any one of claims 1 to 2, characterized in that: The steps include: (1) Weigh the raw materials according to their weight, soak them in water, and heat them for decoction. Filter and take the filtrate; (2) adding water to the filter residue of step (1) for secondary decoction, and filtering to obtain the filtrate; (3) The filtrates from step (1) and step (2) are combined and concentrated to obtain the Chinese medicine composition for improving muscle fat infiltration.

4. The method for preparing the Chinese medicine composition for improving muscle fat infiltration as claimed in claim 3, characterized in that: In the step (1), the amount of water added is 10 times the volume of the prepared raw material.

5. The method for preparing the Chinese medicine composition for improving muscle fat infiltration as claimed in claim 3, characterized in that: In the step (1), the soaking time is 1 hour.

6. The method for preparing the Chinese medicine composition for improving muscle fat infiltration as claimed in claim 3, characterized in that: In the step (2), the amount of water added is 5 times the volume of the prepared raw materials.

7. The method for preparing the Chinese medicine composition for improving muscle fat infiltration as claimed in claim 3, characterized in that: In the steps (1) and (2), the boiling time is 30 minutes.

8. Use of the Chinese medicinal composition for improving muscle fat infiltration according to any one of claims 1 to 2 in the preparation of a medicament for improving muscle fat infiltration.

9. A drug for improving muscle fat infiltration, characterized in that: A Chinese medicine composition for improving muscle fat infiltration comprising any one of claims 1-2.

10. The drug for improving muscle fat infiltration according to claim 9, characterized in that: Pharmaceutically acceptable excipients are also included.

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