A slide staining device for biological analysis

Abstract

The present invention relates to a bioanalysis slide staining device in the field of bioanalysis. Through the clamping cylinders which are equidistantly distributed on a rotating ring and used to fix syringes, when dye and cleaning agent are injected, a power mechanism is used to drive the rotating ring to rotate, and the clamping cylinder drives the syringe to perform circular motion and radial movement, so that the syringe to be injected is moved to just above and close to the sample slide, thereby realizing accurate and rapid switching of syringes storing different dyes or cleaning agents on the rotating ring, and realizing accurate squeezing of the syringes by using an electric telescopic rod and a pressing plate, thereby improving the stability and accuracy of the operation, and the operation is simple and quick.

Description

Technical Field

[0001] The invention relates to a slide staining device, in particular to a slide staining device for biological analysis applied in the field of biological analysis. Background Art

[0002] Sampling analysis is often used in the field of biological analysis, which requires the preparation of biological sample slides, and then observation and analysis using a microscope. When preparing sample slides for biological analysis, the biological samples need to be stained to facilitate color observation.

[0003] Since the internal structure of biological samples is generally complex, in order to improve the color analysis effect, it is generally necessary to use different stains to stain the same sample multiple times. Traditional manual multiple staining is not only cumbersome, but also the poor stability of manual operation affects the staining quality. Summary of the invention

[0004] In view of the above-mentioned prior art, the technical problem to be solved by the present invention is that the traditional biological sample carrier slides need to be stained multiple times during the production process, the manual operation is cumbersome and the stability is poor, which affects the quality of the sample carrier slides.

[0005] In order to solve the above problems, the present invention provides a slide staining device for biological analysis, comprising a base, a clamping mechanism for clamping a sample slide is fixedly connected to the base, a staining mechanism is arranged on the outer side of the sample slide, the staining mechanism comprises a mounting ring, a rotating ring rotatably connected to the mounting ring is nested in the mounting ring, a plurality of clamping tubes radially slidably connected to the rotating ring are inserted into the rotating ring, syringes are inserted into the clamping tubes, some of the syringes are filled with dye, and some of the syringes are filled with cleaning agent; a gear ring is fixedly connected to the annular inner wall of the rotating ring, the gear ring is meshed with a driving gear, the driving gear is connected to the output end of a first motor, a first photoelectric encoder is installed on the output shaft of the first motor, and both the first motor and the first photoelectric encoder are connected to the control The controller is electrically connected; a cylindrical groove for radial sliding of the clamping tube is provided on the rotating ring, a sliding rod is fixedly connected to the lower side wall of the clamping tube, the cylindrical groove is connected to a radial sliding groove for radial sliding of the sliding rod, and the mounting ring is provided with a mounting groove for nesting of the rotating ring, and a guide groove is provided on the inner wall of the mounting groove facing the sliding rod; a first electric telescopic rod is fixedly connected to the top of the mounting ring, a pressing plate for pushing the syringe is fixedly connected to the lower end of the first electric telescopic rod, a first infrared distance sensor is nested in the center of the pressing plate, a second infrared distance sensor is provided above the pressing plate, which is arranged opposite to the pressing plate and fixed to the outer end of the first electric telescopic rod, and the first electric telescopic rod, the first infrared distance sensor and the second infrared distance sensor are all electrically connected to the controller.

[0006] In the above-mentioned slide staining device for biological analysis, automatic and accurate filling of various stains and cleaning agents is achieved through syringes and clamping tubes that are equidistantly distributed on the rotating ring and controlled by a power device.

[0007] As a further improvement of the present application, the clamping mechanism includes a clamping frame sleeved on the outside of the sample slide, a rotating rod is fixedly connected to the outside of the clamping frame, the rotating rod is fixedly connected to the second motor, the second motor is fixedly sleeved with a fixed cylinder, and the fixed cylinder is connected to a second electric telescopic rod.

[0008] As a further improvement of the present application, the clamping cylinder is a vertical cylindrical structure with openings at both ends. A through hole is provided on the rotating ring for the needle tube of the syringe to pass through. The through hole is connected to the clamping cylinder. An elastic bushing is bonded to the inner wall of the clamping cylinder. The elastic bushing is made of elastic material and its inner diameter is smaller than the outer diameter of the barrel of the syringe. After the syringe is inserted into the elastic bushing, it has an interference fit with the elastic bushing.

[0009] As a further improvement of the present application, the guide groove includes an arc-shaped groove and a V-shaped groove connected to the arc-shaped groove and located on the upper side of the center line of the mounting ring.

[0010] As a further improvement of the present application, a waste liquid tank is clamped at the bottom of the mounting ring, and the waste liquid tank is an arc-shaped block with an arc-shaped groove.

[0011] As a further improvement of the present application, a touch display panel is provided at the front end of the controller.

[0012] As a further improvement of the present application, a second photoelectric encoder is installed on the output shaft of the second motor, and a third infrared distance sensor arranged opposite to the fixed tube is fixedly connected to the outer wall of the second electric telescopic rod, and both the second photoelectric encoder and the third infrared distance sensor are electrically connected to the controller.

[0013] As a further improvement of the present application, the clamping frame includes a hollow box body with an opening on one side, a group of relatively arranged clamping plates are nested in the box body, and strip grooves for accommodating sample slides are opened on opposite sides of the two clamping plates. The strip grooves pass through the clamping plates and have rounded corners at the ends. A limiting rod passing through the box body is fixedly connected to the outer side of the clamping plate, and a coil spring is sleeved on the limiting rod, and the coil spring is arranged between the outer wall of the clamping plate and the side wall of the box body.

[0014] A slide staining device for biological analysis, the use method of which comprises the following steps:

[0015] Step 1, fixing the sample slide smeared with the biological sample by a clamping mechanism;

[0016] Step 2: Setting the type, filling amount, standing time, filling sequence and other parameters of the dyeing machine and cleaning agent to be filled through the controller;

[0017] Step 3: The controller starts the first motor, which drives the gear ring and the rotating ring to rotate through the driving gear, and the rotating ring drives the syringe to be filled to move to the top of the sample slide; then the controller starts the first electric telescopic rod, and the first electric telescopic rod pushes the pressing plate downward according to the set filling amount, and the pressing plate pushes the syringe to extrude the dye quantitatively;

[0018] Step 4: After a dye is added, the controller counts the rest time. When the set rest time is reached, the controller starts the first motor again, so that the syringe storing the cleaning agent moves to the top of the sample slide, and then starts the first electric telescopic rod to quantitatively inject the cleaning agent, and then the setting time is left to rest again;

[0019] Step 5: Repeat steps 3 to 4 until all dyes and cleaning agents are automatically filled in sequence.

[0020] To summarize, the present invention uses clamping cylinders that are equidistantly distributed on a rotating ring and used to fix syringes. When injecting dyes and cleaning agents, a power mechanism is used to drive the rotating ring to rotate, and the clamping cylinder drives the syringe to perform circular and radial movements, so that the syringe to be injected is moved to the top of the sample slide and close to the sample slide, thereby achieving accurate and rapid switching of syringes with different dyes or cleaning agents stored on the rotating ring, and using an electric telescopic rod and a pressing plate to achieve precise squeezing of the syringe, thereby improving the stability and accuracy of the operation, and the operation is simple and quick. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the three-dimensional structure from the left side perspective of this application;

[0022] Figure 2 This is a schematic diagram of the three-dimensional structure from the right side perspective of this application;

[0023] Figure 3 It is a cross-sectional structural schematic diagram of the dyeing mechanism in this application;

[0024] Figure 4 This is a schematic diagram of the exploded structure of the assembly of the rotating ring and the syringe in this application;

[0025] Figure 5 It is a schematic diagram of the three-dimensional structure of the clamping tube in this application;

[0026] Figure 6 This is a schematic diagram of the assembly of the mounting ring and the rotating ring in this application;

[0027] Figure 7 It is a schematic diagram of the longitudinal cross-sectional structure of the mounting ring in this application;

[0028] Figure 8This is a schematic diagram of the motion state of the clamping tube in this application;

[0029] Fig. 9 This is a schematic diagram of the transverse cross-sectional structure of the present application;

[0030] Fig.10 for Fig. 9 A schematic diagram of the enlarged structure at A in the middle;

[0031] Fig.11 It is a cross-sectional structural schematic diagram of the clamping mechanism in this application;

[0032] Fig.12 This is a schematic diagram of the exploded structure of the clamping frame in this application;

[0033] Fig.13 It is a schematic diagram of the cross-sectional structure of the flushing head in this application.

[0034] Description of the numbers in the figure:

[0035] 1. Base; 2. Clamping mechanism; 3. Sample slide; 4. Staining mechanism; 5. Controller; 6. Mounting ring; 601. Mounting groove; 602. Guide groove; 6021. Arc groove; 6022. V-shaped groove; 7. Rotating ring; 701. Columnar groove; 702. Radial slide groove; 8. Snap-on cylinder; 801. Sliding rod; 802. Elastic bushing; 9. Syringe; 10. Gear ring; 11. Driving gear; 12. First motor; 13. First photoelectric encoder; 14. Pressing plate; 15. first electric telescopic rod; 16. first infrared distance sensor; 17. second infrared distance sensor; 18. clamping frame; 1801. box body; 1802. clamping plate; 1803. limit rod; 1804. coil spring; 19. rotating rod; 20. fixing cylinder; 21. second motor; 22. second photoelectric encoder; 23. second electric telescopic rod; 24. third infrared distance sensor; 25. flushing head; 2501. spray hole; 26. waste liquid tank. DETAILED DESCRIPTION

[0036] The following describes two implementation modes of the present application in detail with reference to the accompanying drawings.

[0037] The first implementation method:

[0038] Figure 1-10A slide staining device for biological analysis is shown, comprising a base 1, a clamping mechanism 2 for clamping a sample slide 3 is fixedly connected to the base 1, a staining mechanism 4 is arranged on the outer side of the sample slide 3, the staining mechanism 4 comprises a mounting ring 6, a rotating ring 7 rotatably connected to the mounting ring 6 is nested in the mounting ring 6, a plurality of clamping tubes 8 radially slidably connected to the rotating ring 7 are inserted, a syringe 9 is inserted in the clamping tube 8, a part of the syringes 9 are filled with a dye, and a part of the syringes 9 are filled with a cleaning agent; a gear ring 10 is fixedly connected to the annular inner wall of the rotating ring 7, the gear ring 10 is meshed with a driving gear 11, the driving gear 11 is connected to the output end of a first motor 12, a first photoelectric encoder 13 is installed on the output shaft of the first motor 12, and both the first motor 12 and the first photoelectric encoder 13 are electrically connected to a controller 5;

[0039] The rotating ring 7 is provided with a columnar groove 701 for radial sliding of the clamping tube 8, a sliding rod 801 is fixedly connected to the lower side wall of the clamping tube 8, the columnar groove 701 is connected with a radial sliding groove 702 for radial sliding of the sliding rod 801, the mounting ring 6 is provided with a mounting groove 601 for the rotating ring 7 to be nested, and a guide groove 602 is provided on the inner wall of the mounting groove 601 on the side facing the sliding rod 801. When the rotating ring 7 drives the clamping tube 8 to make a circular motion, the sliding rod 801 slides in the guide groove 602. When the clamping tube 8 slides to the top of the sample slide 3, the clamping tube 8 drives the syringe 9 to move downward to approach the sample slide 3;

[0040] A first electric telescopic rod 15 is fixedly connected above the mounting ring 6, a pressing plate 14 for pushing the syringe 9 is fixedly connected to the lower end of the first electric telescopic rod 15, a first infrared distance sensor 16 is nested in the center of the pressing plate 14, a second infrared distance sensor 17 is arranged above the pressing plate 14 and is opposite to the pressing plate 14 and fixed to the outer end of the first electric telescopic rod 15, the first electric telescopic rod 15, the first infrared distance sensor 16 and the second infrared distance sensor 17 are all electrically connected to the controller 5.

[0041] For details, please refer to Figure 6-8 , and its use method includes the following steps:

[0042] Step 1: fix the sample slide 3 smeared with the biological sample by the clamping mechanism 2;

[0043] Step 2, setting the parameters such as the type, filling amount, standing time, filling sequence, etc. of the dyeing machine and cleaning agent to be filled through the controller 5;

[0044] Step 3: The controller 5 starts the first motor 12, which drives the gear ring 10 and the rotating ring 7 to rotate through the driving gear 11, and the rotating ring 7 drives the syringe 9 to be filled to move to the top of the sample slide 3; then the controller 5 starts the first electric telescopic rod 15, and the first electric telescopic rod 15 pushes the pressing plate 14 downward according to the set filling amount, and the pressing plate 14 pushes the syringe 9 to extrude the dye quantitatively;

[0045] It should be noted that the first photoelectric encoder 13 is used to monitor the positions of different syringes 9 on the rotating ring 7, and then the required syringes 9 are moved to the top of the sample slide 3 in a set order; in addition, the first infrared distance sensor 16 detects the distance from the pressing plate 14 to the syringe 9, and the second infrared distance sensor 17 monitors the downward movement distance of the pressing plate 14 after contacting the syringe 9, so as to accurately control the injection volume;

[0046] Step 4: After one dye is added, the controller 5 counts the rest time. When the set rest time is reached, the controller 5 starts the first motor 12 again, so that the syringe 9 storing the cleaning agent moves to the top of the sample slide 3, and then starts the first electric telescopic rod 15 to quantitatively inject the cleaning agent, and then the cleaning agent is left to rest for the set time again.

[0047] Step 5: Repeat steps 3 to 4 until all dyes and cleaning agents are automatically filled in sequence.

[0048] Compared with the traditional manual operation of staining biological sample slides multiple times, the present invention uses a rotating ring 7 and a clamping tube 8 installed on the rotating ring 7 to fix the syringe 9. When performing multiple staining operations, different types of dyes and cleaning agents are automatically and quickly switched, and the pressing plate 14 and the first electric telescopic rod 15 are used to realize the extrusion injection of the syringe 9; in addition, the first photoelectric encoder 13 is used to detect the position of the syringe 9 storing different types of injections, and the first infrared distance sensor 16 and the second infrared distance sensor 17 are used to accurately add the injection, so the operation is stable, accurate and efficient.

[0049] See also Figure 3-5 The clamping cylinder 8 is a vertical cylindrical structure with openings at both ends. A through hole is provided on the rotating ring 7 for the needle tube of the syringe 9 to pass through. The through hole is connected to the clamping cylinder 8. An elastic bushing 802 is bonded to the inner wall of the clamping cylinder 8. The elastic bushing 802 is made of elastic material and its inner diameter is smaller than the outer diameter of the barrel of the syringe 9. After the syringe 9 is inserted into the elastic bushing 802, it has an interference fit with it.

[0050] Specifically, the elastic bushing 802 is provided to facilitate disassembly, assembly and replacement of the syringe 9, thereby satisfying dyeing operations with different dyes.

[0051] See also Figure 7 The guide groove 602 includes an arc groove 6021 and a V-shaped groove 6022 connected to the arc groove 6021 and located on the upper side of the center line of the mounting ring 6.

[0052] Specifically, when the sliding rod 801 rotates to the top of the mounting ring 6, it moves from the arc groove 6021 to the V-shaped groove 6022, and the sliding rod 801 drives the clamping cylinder 8 to rotate in a circle and move in a radial direction at the same time.

[0053] See also Figure 3 A waste liquid tank 26 is clamped at the bottom of the mounting ring 6, and the waste liquid tank 26 is an arc-shaped block with an arc-shaped groove.

[0054] Specifically, it is convenient to collect dripping dyes and cleaning agents.

[0055] In this embodiment, a touch display panel is provided at the front end of the controller 5 .

[0056] Specifically, it is convenient to set parameters and realize automated multiple staining operations.

[0057] Second implementation method:

[0058] Figure 11-13 A slide staining device for biological analysis is shown. Based on the first embodiment, the clamping mechanism 2 includes a clamping frame 18 sleeved on the outside of the sample slide 3, a rotating rod 19 is fixedly connected to the outside of the clamping frame 18, the rotating rod 19 is fixedly connected to a second motor 21, the second motor 21 is fixedly sleeved with a fixed cylinder 20, and the fixed cylinder 20 is connected to a second electric telescopic rod 23.

[0059] Specifically, the second motor 21 drives the clamping frame 18 to rotate through the rotating rod 19, which is convenient for adjusting the inclination angle of the sample slide 3, and facilitates the flow of the cleaning agent to one side during cleaning, thereby improving the cleaning effect. After cleaning is completed, the cleaning agent is reciprocated to increase the speed of separation and reduce the static volatilization time. In addition, the second electric telescopic rod 23 drives the clamping frame 18 to move horizontally through the fixed cylinder 20 and the rotating rod 19, which is convenient for adjusting the lateral position of the sample slide 3, facilitating the disassembly and assembly of the sample slide 3, and adjusting the filling position of the stain or cleaning agent, thereby improving the accuracy of the filling.

[0060] See also Fig.11 A second photoelectric encoder 22 is installed on the output shaft of the second motor 21, and a third infrared distance sensor 24 arranged opposite to the fixed tube 20 is fixedly connected to the outer wall of the second electric telescopic rod 23. Both the second photoelectric encoder 22 and the third infrared distance sensor 24 are electrically connected to the controller 5.

[0061] Specifically, the controller 5 monitors the tilt angle and the lateral position of the sample slide 3 to facilitate accurate adjustment.

[0062] See also Fig.12 The clamping frame 18 includes a hollow box body 1801 with an opening on one side, and a group of relatively arranged clamping plates 1802 are nested in the box body 1801. The two clamping plates 1802 have opposite sides with strip grooves for accommodating the sample slide 3. The strip grooves penetrate the clamping plates 1802 and have rounded corners at the ends. A limiting rod 1803 penetrating the box body 1801 is fixedly connected to the outer side of the clamping plate 1802. A coil spring 1804 is sleeved on the limiting rod 1803, and the coil spring 1804 is arranged between the outer wall of the clamping plate 1802 and the side wall of the box body 1801.

[0063] Specifically, when installing the sample slide 3, the sample slide 3 is horizontally inserted into the strip groove of the clamping plate 1802, and the spiral spring 1804 is squeezed to achieve squeezing and fixing of the sample slide 3, which facilitates the disassembly and installation of the sample slide 3.

[0064] See also Fig.13 A flushing head 25 is threadedly connected to the lower end of the needle tube of the syringe 9 filled with the cleaning agent, and the lower end of the flushing head 25 is provided with spray holes 2501 distributed equidistantly around the circumference.

[0065] Specifically, after the cleaning agent enters the rinse head 25, it is sprayed out along the spray hole 2501, so that the sprayed water flow is evenly distributed and avoids direct impact on the dyeing position, thereby avoiding damage to the dyeing sample.

[0066] In view of current practical needs, the above-mentioned implementation mode adopted in this application is not limited to the scope of protection. Various changes made within the knowledge scope of technical personnel in this field without departing from the concept of this application still fall within the scope of protection of the present invention.

Claims

1. A slide staining device for biological analysis, characterized in that: The invention comprises a base (1), a clamping mechanism (2) for clamping a sample glass slide (3) is fixedly connected to the base (1), a dyeing mechanism (4) is arranged outside the sample glass slide (3), the dyeing mechanism (4) comprises a mounting ring (6), a rotating ring (7) is nested in the mounting ring (6) and connected to the rotating ring for rotation, a plurality of clamping cylinders (8) are inserted into the rotating ring (7) and connected to the rotating ring for radial sliding, a syringe (9) is inserted into the clamping cylinder (8), a part of the syringes (9) are filled with dye, and a part of the syringes (9) are filled with cleaning agent; a gear ring (10) is fixedly connected to the annular inner wall of the rotating ring (7), the gear ring (10) is meshed with a driving gear (11), the driving gear (11) is connected to the output end of a first motor (12), a first photoelectric encoder (13) is installed on the output shaft of the first motor (12), and both the first motor (12) and the first photoelectric encoder (13) are electrically connected to a controller (5); The rotating ring (7) is provided with a columnar groove (701) for radial sliding of the clamping cylinder (8); a sliding rod (801) is fixedly connected to the lower side wall of the clamping cylinder (8); the columnar groove (701) is connected to a radial sliding groove (702) for radial sliding of the sliding rod (801); the mounting ring (6) is provided with a mounting groove (601) for the rotating ring (7) to be nested; a guide groove (602) is provided on the inner wall of the mounting groove (601) on the side facing the sliding rod (801); A first electric telescopic rod (15) is fixedly connected to the top of the mounting ring (6); a pressing plate (14) for pushing the syringe (9) is fixedly connected to the bottom of the first electric telescopic rod (15); a first infrared distance sensor (16) is embedded in the center of the pressing plate (14); a second infrared distance sensor (17) is arranged above the pressing plate (14) and is directly opposite to the pressing plate (14) and fixed to the outer end of the first electric telescopic rod (15); the first electric telescopic rod (15), the first infrared distance sensor (16) and the second infrared distance sensor (17) are all electrically connected to the controller (5); The clamping mechanism (2) comprises a clamping frame (18) sleeved on the outside of the sample glass slide (3); a rotating rod (19) is fixedly connected to the outside of the clamping frame (18); the rotating rod (19) is fixedly connected to a second motor (21); the second motor (21) is fixedly sleeved with a fixing cylinder (20); the fixing cylinder (20) is connected to a second electric telescopic rod (23); By means of the clamping cylinders (8) which are equidistantly distributed on the rotating ring (7) and used to fix the syringes (9), when the dye and cleaning agent are injected, the rotating ring (7) is driven to rotate by the power mechanism, and the clamping cylinders (8) drive the syringes (9) to perform circular motion and radial movement, so that the syringe (9) to be injected is moved to the top of the sample glass slide (3) and close to the sample glass slide (3), thereby realizing accurate and rapid switching of the syringes (9) storing different dyes or cleaning agents on the rotating ring (7), and realizing accurate squeezing of the syringes (9) by means of the electric telescopic rod and the pressing plate (14).

2. A bioanalysis slide staining device according to claim 1, characterized in that: The clamping cylinder (8) is a vertical cylindrical structure with openings at both ends. A through hole is provided on the rotating ring (7) for the needle tube of the syringe (9) to pass through. The through hole is connected to the clamping cylinder (8). An elastic bushing (802) is bonded to the inner wall of the clamping cylinder (8). The elastic bushing (802) is made of elastic material and has an inner diameter smaller than the outer diameter of the barrel of the syringe (9). After the syringe (9) is inserted into the elastic bushing (802), it is interference-fitted with the syringe (9).

3. A bioanalysis slide staining device according to claim 1, characterized in that: The guide groove (602) comprises an arc-shaped groove (6021) and a V-shaped groove (6022) which is connected to the arc-shaped groove (6021) and is located on the upper side of the center line of the mounting ring (6).

4. A bioanalysis slide staining device according to claim 1, characterized in that: A waste liquid tank (26) is clamped at the bottom of the mounting ring (6), and the waste liquid tank (26) is an arc-shaped block provided with an arc-shaped groove.

5. A bioanalysis slide staining device according to claim 1, characterized in that: The controller (5) is provided with a touch display panel at the front end.

6. A bioanalysis slide staining device according to claim 1, characterized in that: A second photoelectric encoder (22) is mounted on the output shaft of the second motor (21); a third infrared distance sensor (24) disposed opposite to the fixed cylinder (20) is fixedly connected to the outer wall of the second electric telescopic rod (23); and both the second photoelectric encoder (22) and the third infrared distance sensor (24) are electrically connected to the controller (5).

7. A bioanalysis slide staining device according to claim 1, characterized in that: The clamping frame (18) comprises a hollow box body (1801) with an opening on one side, a group of clamping plates (1802) arranged opposite to each other are nested in the box body (1801), and strip grooves for accommodating sample slides (3) are provided on opposite sides of the two clamping plates (1802), the strip grooves pass through the clamping plates (1802) and have rounded corners at the ends, and a limiting rod (1803) passing through the box body (1801) is fixedly connected to the outer side of the clamping plate (1802), and a coil spring (1804) is sleeved on the limiting rod (1803), and the coil spring (1804) is arranged between the outer wall of the clamping plate (1802) and the side wall of the box body (1801).

8. A bioanalysis slide staining device according to claim 1, characterized in that: The method of use includes the following steps: Step 1: fix the sample glass slide (3) smeared with the biological sample by means of a clamping mechanism (2); Step 2, setting the type, filling amount, standing time, filling sequence and other parameters of the dyeing machine and cleaning agent to be filled through the controller (5); Step 3: The controller (5) starts the first motor (12), and the first motor (12) drives the gear ring (10) and the rotating ring (7) to rotate via the driving gear (11), and the rotating ring (7) drives the syringe (9) to be filled to move to the top of the sample slide (3); then the controller (5) starts the first electric telescopic rod (15), and the first electric telescopic rod (15) pushes the pressing plate (14) downward according to the set filling amount, and the pressing plate (14) pushes the syringe (9) to extrude the dye quantitatively; Step 4: After a dye is added, the controller (5) counts the rest time. When the set rest time is reached, the controller (5) starts the first motor (12) again, so that the syringe (9) storing the cleaning agent is moved to the top of the sample slide (3), and then starts the first electric telescopic rod (15) to quantitatively inject the cleaning agent, and then the cleaning agent is left to rest again for a set time. Step 5: Repeat steps 3 to 4 until all dyes and cleaning agents are automatically filled in sequence.

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