Moisturizing repair firming composition and its use in cosmetics

Abstract

The application belongs to the technical field of cosmetics, and particularly relates to a moisturizing, repairing and firming composition and application thereof in cosmetics. Specifically, the composition is prepared by compounding sodium hyaluronate disaccharide, quinoa bran extract and artemisia capillaris extract composition, and radix peucedani extract; and then the composition is used as a main effective component to prepare skin care cosmetics. It is verified by experiments that the composition has good effects of improving skin relaxation and wrinkles, can be used in cosmetics to improve skin wrinkles and relaxation, and has a good application prospect in cosmetics.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a moisturizing and repairing firming composition and application thereof in cosmetics. BACKGROUND

[0002] The information disclosed in the background of the present application is only intended to increase the understanding of the overall background of the present application, and is not necessarily regarded as acknowledging or implying in any form that the information constitutes prior art known to those skilled in the art.

[0003] Skin, as the largest organ of the human body, is composed of epidermis, dermis and subcutaneous tissue, and is a kind of multilayer, highly nonlinear and anisotropic viscoelastic material, each layer has its own composition and mechanical properties. There is a layer called dermal-epidermal junction (DEJ) between the dermis and the epidermis. It is a dense collagen network that forms a corrugated dermal-epidermal junction structure, which provides good structural support for the epidermis and dermis. DEJ controls the structural integrity of the skin and plays multiple roles in skin homeostasis and function. Epidermal cells continuously move upwards, form a stratum corneum in the epidermis, and finally fall off. DEJ can enhance the adhesion and physical interlocking of the epidermis, create niches for epidermal stem cells, and regulate the cell microenvironment; in addition, as a physical boundary layer between fibroblasts and keratinocytes, it provides an exchange barrier for dynamic balance signal transduction. DEJ is also responsible for the exchange of nutrients between the epidermis and the dermis. As a complex signal platform, DEJ controls dermal-epidermal signals and plays an important role in maintaining healthy epidermis and dermis. Without DEJ to maintain the relationship between the epidermis and the dermis, it is impossible to achieve healthy signal exchange from top to bottom.

[0004] Research has found that the application of some active substances that can promote the content of DEJ core components, such as collagen type XVII, collagen type IV, collagen type VII and laminin 332, in daily chemical products can have a good effect on maintaining the structure and homeostasis of DEJ. Collagen type IV, which exists in DEJ, is the most abundant collagen fiber in the dense layer and is the main component. Collagen type IV is produced by keratinocytes and fibroblasts and is the main component of anchoring fibrils, which provides mechanical support for keratinocytes. Collagen type XVII is another structural component of anchoring filaments and plays an important role in the assembly and function of cell matrix adhesion structures, and plays an important role in transmembrane signal transduction and keratinocyte differentiation. Deficiency of collagen type XVII gene can cause skin atrophy and a decrease in the number of epidermal stem cells. DEJ is a good target for skin care products, and ingredients that enhance DEJ repair can improve epidermal-dermal communication and skin homeostasis, thereby strengthening the defense against skin aging.

[0005] Skin barrier plays an important role in preventing trans-epidermal water loss (TEWL), penetration of external immunogens and microorganisms, etc. The stratum corneum, as the outermost layer of the skin barrier, is composed of keratinocytes embedded in intercellular lipid. Natural moisturizing factor (NMF) synthesized in keratinocytes is a key component of skin stratum corneum moisturizing, which is completely degraded into free amino acids by filaggrin (FLG) under the hydrolysis of caspase-14, and finally forms natural moisturizing factor to maintain the relative humidity of the skin. There is a layer of protein shell called corneocyte envelope (CE) around each keratinocyte, and the content of Loricrin (LOR) in the CE directly affects the strength of the skin barrier.

[0006] However, the inventors found that the cosmetics on the market with moisturizing, repairing and firming effects have a single action pathway or poor firming effect. Therefore, there is a need to develop a cosmetic that effectively firms the skin, moisturizes and repairs to improve the problems of dry, red, loose and wrinkled skin. SUMMARY

[0007] In view of the deficiencies of the prior art, the present application provides a moisturizing, repairing and firming composition and its application in cosmetics. Specifically, the present application combines sodium hyaluronate disaccharide, quinoa bran extract and artemisia capillaris extract composition, and radix peucedani extract to prepare a composition; then the composition is used as the main effective ingredient to prepare a skin care cosmetic. It has been verified through experiments that the composition has good effects of improving skin relaxation and wrinkles, and can be used in cosmetics to improve the problems of skin wrinkles and relaxation, thus having good application prospects in the field of cosmetics.

[0008] In order to achieve the above technical purpose, the technical scheme provided by the present application is as follows:

[0009] In a first aspect of the present application, a moisturizing, repairing and firming composition is provided, which comprises the following components in mass fraction:

[0010] sodium hyaluronate disaccharide 0.01-1.0 parts, quinoa bran extract and artemisia capillaris extract composition 1.0-15.0 parts, radix peucedani extract 1.0-15.0 parts;

[0011] Further, the moisturizing, repairing and firming composition comprises the following components in mass fraction:

[0012] sodium hyaluronate disaccharide 0.1-0.6 parts, quinoa bran extract and artemisia capillaris extract composition 5.0-10.0 parts, radix peucedani extract 5.0-10.0 parts.

[0013] The sodium hyaluronate disaccharide can be obtained by commercial means.

[0014] The extract of the Cnidium monnieri is prepared by the following method:

[0015] S1, after the Cnidium monnieri is crushed and sieved, it is immersed in ethanol for ultrasonic extraction, filtered, and the filtrate is collected, and the residue is extracted repeatedly for 2-3 times, and the obtained filtrates are combined;

[0016] S2, the filtrate obtained in step S1 is concentrated under reduced pressure, and ethanol is recovered until there is no alcohol taste, and butanediol is added thereto, stirred uniformly, and filtered to obtain the filtrate;

[0017] In step S1, the sieving treatment is selected from a 30-60 mesh sieve;

[0018] The ethanol is selected from 60%-85% ethanol, the solid-liquid ratio is 1:8-1:10 (g / mL), and the soaking time is controlled to be 20-40 min; during the ultrasonic extraction process, the ultrasonic power is 100-300 W, and the ultrasonic treatment time is 40-60 min;

[0019] In step S2, the reduced pressure concentration is specifically as follows: the ethanol is recovered to no alcohol taste under reduced pressure distillation treatment at 50-60°C to obtain a Cnidium monnieri concentrate, 2-2.5 times the mass of butanediol is added to the Cnidium monnieri concentrate, stirred uniformly, and filtered to obtain the filtrate.

[0020] The quinoa bran extract and the Artemisia capillaris extract composition can be prepared by any one of the following preparation methods:

[0021] Specifically, the extraction method 1: (in the present application, the quinoa bran extract and the Artemisia capillaris extract composition obtained by this method are referred to as KNYC① composition), and the specific method is as follows:

[0022] S1, crude extraction: after the Artemisia capillaris is dried, crushed and sieved, water is added for ultrasonic atomization extraction to obtain an Artemisia capillaris crude extract;

[0023] S2, deacidification: the Artemisia capillaris crude extract obtained in step S1 is filtered, and neutral aluminum oxide is added thereto, and the solid is filtered out to obtain an Artemisia capillaris extract;

[0024] S3, adsorption: the Artemisia capillaris extract is subjected to adsorption treatment using a macroporous resin;

[0025] S4, preparation of eluent: the quinoa bran is mixed with butanediol, and microwave heating low-temperature extraction is carried out, the solid is filtered out, the filtrate is diluted with water to obtain an eluent;

[0026] S5, elution: the eluent obtained in step S4 is used to elute the macroporous resin to which the Artemisia capillaris extract is adsorbed in step S3 to obtain a refined extract;

[0027] S6, purification: the purified extract obtained in step S5 is filtered and purified to obtain the KNYC① composition.

[0028] In step S1, the particle size of the dried and crushed Artemisia capillaris is between 50 and 120 mesh, the mass ratio of the sieved Artemisia capillaris particles to water is 1:6-10 (preferably 1:8), the ultrasonic atomization extraction temperature is controlled at 25-40°C, and the extraction time is 30-35 min.

[0029] In step S2, the mesh number of the neutral alumina is 80-150 mesh, preferably 100 mesh.

[0030] In step S3, the macroporous resin is any one or more of D101, AB-8, and HPD-100, preferably AB-8.

[0031] Further, the macroporous resin is placed in a separation column, which can be a single column or multiple columns connected in series.

[0032] In step S4, the microwave heating extraction temperature is 45-55°C, and the extraction time is 90-100 min.

[0033] Meanwhile, the quinoa bran extract and Artemisia capillaris extract composition can also be obtained by direct mixing extraction in one step, i.e., extraction method 2, (in the present application, the quinoa bran extract and Artemisia capillaris extract composition obtained by this method is referred to as KNYC② composition), and the specific method is as follows:

[0034] S1, after the quinoa bran and Artemisia capillaris are dried and crushed, they are immersed in an ethanol solution for ultrasonic-microwave synergistic extraction, the extract is centrifuged, the supernatant is collected, the filtrate is collected by vacuum filtration, and the residue is extracted repeatedly 2-3 times, and the obtained filtrates are combined;

[0035] S2, the filtrate obtained in step S1 is concentrated under reduced pressure, glycerol is added after concentration, stirred uniformly, and filtered to obtain the product.

[0036] In step S1, the quinoa bran powder is sieved with a 30-60 mesh sieve, and the Artemisia capillaris powder is sieved with a 40-100 mesh sieve; the feeding ratio (mass ratio) of the quinoa bran powder and the Artemisia capillaris powder is 1:0.9-1:1.1.

[0037] The ethanol solution is selected from 50%-80% ethanol solution, the solid-liquid ratio is 1:45-1:60 (g / mL), ultrasonic-microwave synergistic extraction is performed, the ultrasonic power is 100-200 W, the microwave power is 400-500 W, the treatment temperature is 25-45°C, and the treatment time is 1-3 min.

[0038] In the step S2, the reduced pressure concentration is specifically reduced pressure distillation treatment at 50-60 DEG C, and the concentration is 4-6 times of the mass of the quinoa bran and the Artemisia capillaris powder, and the same mass of glycerol as the concentrated solution is added and uniformly stirred.

[0039] The present application is verified by experiments, and the above-mentioned composition can promote the synthesis of collagen type IV and collagen type XI, promote the expression of LOR, and promote the expression of Caspase-14, so as to improve the dryness, redness, relaxation and wrinkles of the skin, and has no toxic side effects. Therefore, the second aspect of the present application provides the use of the above-mentioned moisturizing and repairing and tightening composition in the preparation of cosmetics. Obviously, the cosmetics have the effects of improving the dryness, redness, relaxation and wrinkles of the skin. The cosmetics can be skin care products.

[0040] The third aspect of the present application provides a cosmetic for improving the dryness, redness, relaxation and wrinkles of the skin, which comprises at least the above-mentioned moisturizing and repairing and tightening composition. The cosmetic can be a skin care product.

[0041] The cosmetic according to the present application, wherein the amount of the oil control and soothing skin care moisturizing and repairing and tightening composition is 0.5-15%, preferably 2-8%, such as 1%, 3%, 5%, 7%, 10%, 12%, 14% or 15%, based on the total mass of the cosmetic.

[0042] The cosmetic can also comprise other raw material ingredients allowed to be added in the field of cosmetics, including but not limited to emulsifiers, emollients, moisturizers, thickeners and preservatives, etc.

[0043] Meanwhile, the present application can also prepare different cosmetic dosage forms such as essence water, essence milk, essence cream, etc. by reasonably adding the above-mentioned raw material ingredients, and based on the above-mentioned basic cosmetic categories, further derived other cosmetic categories are obviously within the protection scope of the present application.

[0044] The fourth aspect of the present application provides a preparation method of the above-mentioned cosmetic, which comprises the step of mixing the moisturizing and repairing and tightening composition with other raw material components. This is not specifically limited.

[0045] The above-mentioned one or more technical solutions have the following beneficial technical effects:

[0046] (1) The moisturizing and repairing and tightening composition obtained by the above-mentioned technical solution can significantly promote the synthesis of collagen type IV and collagen type XI, promote the synthesis of LOR and Caspase-14, so as to achieve the effect of effective moisturizing, repairing and tightening;

[0047] (2) The cost of sodium hyaluronate disaccharide is high, and the sodium hyaluronate disaccharide, KNYC ① and the peucedanum decursivum extract are used in combination in the application, so that the use amount of the sodium hyaluronate disaccharide is lower when achieving similar or more significant effects of improving and promoting type IV and type XVII collagen, and the cost is greatly saved;

[0048] (3) It is verified through experiments that the KNYC ① composition has better skin care efficacy than the KNYC ② composition, so that the product has higher efficacy and is more cost-saving.

[0049] (4) The preparation method of the skin care cosmetic prepared by the above technical scheme is simple and easy to implement, cost-saving, has good effects of improving and promoting skin relaxation and wrinkles through human efficacy experiment tests, and therefore has good practical application value. DETAILED DESCRIPTION

[0050] It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the application. Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as would be understood by one of ordinary skill in the art to which the application pertains.

[0051] It should be noted that the terms used herein are only for the purpose of describing specific embodiments, and are not intended to limit the exemplary embodiments according to the application. As used herein, the singular form is intended to include the plural form unless the context clearly indicates otherwise, and furthermore, it should be understood that when the terms "comprise" and / or "include" are used in the specification, there is a presence of the features, steps, operations, devices, components and / or combinations thereof.

[0052] The application will be further described in conjunction with specific examples, and the following examples are only for the purpose of explaining the application and do not limit the content thereof. If the specific conditions of the experiments are not specified in the examples, they are usually carried out under conventional conditions or according to the conditions recommended by the reagent companies; the reagents, consumables, etc. used in the following examples can be obtained from commercial channels if not otherwise specified.

[0053] The application will be further described in conjunction with specific examples, and the following examples are only for the purpose of explaining the application and do not limit the content thereof. If the specific conditions of the experiments are not specified in the examples, they are usually carried out under conventional conditions or according to the conditions recommended by the reagent companies; the reagents, consumables, etc. used in the following examples can be obtained from commercial channels if not otherwise specified.

[0054] The KNYC ① composition in the examples and comparative examples of the application is obtained by the following method:

[0055] S1, crude extraction. After the dry Artemisia capillaris is crushed and sieved twice with 50-mesh and 120-mesh sieves, 10.0 kg of fine powder with a particle size between 50-mesh and 120-mesh is weighed, and 80.0 kg of water is used as the extraction liquid to perform atomization extraction. The extraction temperature is 35℃, and the extraction time is 30 min. After the residue is removed by filtration, the crude Artemisia capillaris extract is obtained;

[0056] S2, deacidification. The crude Artemisia capillaris extract is filtered, and then 100-mesh neutral alumina 0.5 kg is added thereto, and stirred for more than 60 min. The solid is removed by filtration to obtain the Artemisia capillaris extract;

[0057] S3, adsorption. Two-stage serial separation columns are used, and the resin filling height of each stage is not less than 500 mm. AB-8 macroporous adsorption resin with a particle size of 0.4-1.0 mm is used, and the total weight of the resin filling of the two-stage separation columns is 15 kg. The Artemisia capillaris extract is injected from the top end of the first-stage separation column, flows to the top end of the second-stage separation column through the first-stage separation column, and flows out from the bottom end of the second-stage separation column. The flow rate is controlled so that the adsorption time (from the initial injection to the complete elution) is 30-35 min;

[0058] S4, preparation of the eluent. 16.0 kg of particle size less than 100-mesh of chenopodium quinoa bran is taken, 80.0 kg of butanediol is added, and the mixture is thoroughly mixed. Microwave heating is used, the extraction temperature is controlled at 50℃, and the extraction time is 95 min. After filtration, the chenopodium quinoa bran extract is obtained, and the weight is 86.2 kg. 38.8 kg of water is added to make the total weight of the eluent reach 125.0 kg;

[0059] S5, elution. The eluent obtained in step S4 is injected from the top end of the first-stage separation column obtained in step S3, and flows out from the bottom end of the second-stage separation column to perform elution. The total elution time is not less than 30 min, and the refined extract is obtained;

[0060] S6, purification. The refined extract obtained in step S5 is filtered with a 0.22-micron polypropylene membrane, and the weight of the filtrate is 114.0 kg. The product obtained is the KNYC① composition. The total phenylpropanoid concentration of the product is 0.535% W / W, and the total phenylpropanoid content in the product is calculated to be 609.9 g.

[0061] The KNYC② composition in the examples and comparative examples of the present application is prepared by the following method:

[0062] S1, dry Artemisia capillaris to constant temperature, crush and sieve twice with 40 mesh and 100 mesh sieves, and take the fine powder with a particle size between 40 mesh and 100 mesh; dry quinoa bran to constant temperature, crush and sieve with a 60 mesh sieve, and then immerse the sieved quinoa bran powder and Artemisia capillaris powder in an 80% ethanol solution according to a mass ratio of 1:1 for ultrasonic-microwave synergistic extraction, centrifuge the extraction liquid, take the supernatant, and reduce pressure to filter the filtrate; the material-liquid ratio of the quinoa bran powder and Artemisia capillaris powder to the ethanol solution is 1:50 g / mL; during the ultrasonic-microwave synergistic extraction, the ultrasonic power is 150 W, the microwave power is 400 W, the treatment temperature is 35℃, and the treatment time is 2 min;

[0063] S2, concentrate the filtrate obtained in step S1 at 50℃ under reduced pressure, concentrate to 5 times the mass of the quinoa bran and Artemisia capillaris powder, add glycerol in an amount equal to the mass of the concentrated liquid, stir uniformly, and filter to obtain the product.

[0064] In the examples and comparative examples of the present application, the radix peucedani extract is prepared by the following method:

[0065] S1, crush radix peucedani, immerse in an 80% ethanol solution for 30 min after sieving with a 50 mesh sieve, then perform ultrasonic extraction, filter, collect the filtrate, repeat the extraction of the residue twice, and combine the obtained filtrates; the material-liquid ratio of the radix peucedani powder to the ethanol is 1:10 g / mL, and the ultrasonic power is 200 W during the ultrasonic extraction, and the ultrasonic treatment time is 50 min.

[0066] S2, concentrate the filtrate obtained in step S1 at 50℃ under reduced pressure, concentrate to 5 times the mass of the quinoa bran and Artemisia capillaris powder, add glycerol in an amount equal to the mass of the concentrated liquid, stir uniformly, and filter to obtain the product.

[0067] Examples 1-3 and Comparative Examples 1-8:

[0068] The specific scheme is shown in Table 1 below, calculated by mass fraction. The preparation method is as follows: mix hyaluronic acid disaccharide, KNYC① composition, KNYC② composition and radix peucedani extract together, and dissolve in purified water.

[0069] Table 1 lists the ingredients of specific examples and comparative examples

[0070]

[0071]

[0072] Example 4: Moisturizing repair and firming serum

[0073] The above composition is used to prepare the essence cream product containing the moisturizing and firming composition, and the formula is shown in Table 2.

[0074] Table 2 Formula of the essence cream product

[0075]

[0076] Preparation method of the essence cream:

[0077] S1: Preparation of the water phase: the raw materials in phase A are added respectively, and heated to 80-85℃ for stirring and dissolving;

[0078] S2: Preparation of the oil phase: the raw materials in phase B are added respectively, and heated to 75-80℃ for melting;

[0079] S3: Combine phase A and phase B, and start homogenization (3000 rpm) for 12 min, and start cooling;

[0080] S4: When the temperature drops to 65-70℃, add phase C, and stir and dissolve, and continue to cool after dissolution;

[0081] S5: When the temperature drops to 40-45℃, add the ingredients in phase D, and stir and dissolve and disperse uniformly, and the essence cream product is obtained.

[0082] Test Example 1: Stability test

[0083] The samples of Examples 1-3 and Comparative Examples 1-8 are placed at room temperature, -18℃, 4℃, 45℃, and cold-heat cycle (-18℃, 4℃, 45℃, cycle once every 3 days) for corresponding time for stability observation, and the stability of the product is determined by whether there is precipitation phenomenon, if there is no precipitation phenomenon, it is stable, and the specific results are shown in Table 3.

[0084] Table 3 Stability test results

[0085]

[0086] Note: √ means stable, no precipitation phenomenon; × means slight precipitation phenomenon; ×× means serious precipitation phenomenon; ○ means the color of the solution changes, the color becomes slightly darker; ○ means the color of the solution changes, the color becomes darker seriously.

[0087] As can be seen from Table 3, KNYC① composition and KNYC② composition are unstable when they exist alone. However, for KNYC① composition, when it exists simultaneously with sodium hyaluronate and radix peucedani extract, it can pass the stability test. However, for KNYC② composition, when it is mixed with sodium hyaluronate and radix peucedani extract, it cannot pass the stability test.

[0088] Test Example 2: Experiment of promoting expression of Collagen IV and Collagen XlV genes

[0089] Human skin fibroblasts (HSF) were used as a model cell line for the experiment. The cells were inoculated into 6-well plates and incubated at 37°C in a 5% CO2 incubator overnight. A blank control group (BC), a positive control group (PC), and a sample group were set up for each plate. When the cell plating rate reached 40-60% in the 6-well plate, the cells were divided into groups and dosed. The BC group was added with 2 mL of culture medium per well, the PC group was added with 2 mL of culture medium containing TGF-β1 (DMEM, Gibco) per well, and the sample group was added with the test sample according to the test scheme (Table 4). After dosing, the 6-well plates were placed in a CO2 incubator for 24 h. The old liquid was aspirated, washed twice with PBS, 1 mL of RNAiso Plus (Aikangrui Biological Engineering Co., Ltd.) was added to each well, and the cells were lysed by blowing. The sample was collected to extract RNA, and after reverse transcription to cDNA, fluorescence quantitative PCR detection was performed. The results were calculated using the 2- △△CT Method.

[0090]

[0091] GraphPad Prism was used for plotting, and the results were expressed as Mean ± SD. The t-test statistical analysis was used for comparison between groups. The statistical analysis was two-tailed. 0.01 < P < 0.05 was considered to have a significant difference, and P < 0.01 was considered to have a very significant difference. The mRNA amplification fold of the BC group was normalized, and compared with the BC group, the significance * was represented as P-value < 0.05, and P-value < 0.01 was represented as **.

[0092] Table 4 Test scheme

[0093]

[0094] Note: The sample concentration was 1 mg / mL (diluted with deionized water).

[0095] Table 5 Summary of Collagen IV gene content detection results

[0096]

[0097]

[0098] (Note: ## indicates p < 0.01 compared with the blank control; ** indicates p < 0.01 compared with the blank control; * indicates 0.01 < p < 0.05 compared with the blank control.)

[0099] From Table 5, it can be seen that the Collagen IV gene content of the PC group increased significantly compared with the BC group, indicating that the positive control test was effective. The single sodium hyaluronate disaccharide component and the extract of Peucedanum praeruptorum did not have the function of promoting the expression of the Collagen IV gene. Comparative Example 9 contained both the sodium hyaluronate disaccharide and the extract of Peucedanum praeruptorum, and the up-regulation rate of the Collagen IV gene was 10.0%, and the two had a certain synergistic effect. Examples 1-3 all contained the sodium hyaluronate disaccharide and the extract of Peucedanum praeruptorum KNYC composition, and the up-regulation rates were 54.0%, 156.0% and 121.0%, respectively, and the three had a significant synergistic promotion effect. It can also be seen that the efficacy of the KNYC composition is stronger than that of the KNYC composition, and the synergistic promotion effect is also higher than that of the KNYC composition.

[0100] Table 6: Collagen XI I gene content detection result summary table

[0101]

[0102]

[0103] (Note: ## indicates p<0.01 compared with the blank control; ** indicates p<0.01 compared with the blank control; * indicates 0.01<p<0.05 compared with the blank control.)

[0104] From Table 6, it can be seen that the Collagen XI I gene content of the PC group increased significantly compared with the BC group, indicating that the positive control test was effective. Compared with the BC group, the single KNYC composition did not have the ability to promote the expression of the Collagen XI I gene. Examples 1-3 and Comparative Examples 1, 4, 5, 6, 7 and 8 had a certain ability to promote the expression of the Collagen XI I gene. It can also be shown that the composition has the function of synergistically promoting the expression of the Collagen XI I gene. It can also be seen that the efficacy of the KNYC composition is stronger than that of the KNYC composition, and the synergistic promotion effect is also higher than that of the KNYC composition.

[0105] Test Example 3: Experiment of promoting the expression of Caspase-14 and LOR genes

[0106] After reviving keratinocytes, when the cell deposition rate reached approximately 60%, cells were seeded into 6-well plates and incubated overnight at 37°C in a 5% CO2 incubator. Cells were then seeded into 24-well plates, and when the cell deposition rate reached 40%–60%, the cells were divided into groups for drug administration, with three replicates per group. Each plate contained a blank control group (BC), a positive control group (PC), and a sample group, configured according to Table 7. 2 mL of culture medium was added to each well of the blank control group, 2 mL of culture medium containing WY14643 (Sigma) (KcGrowth, Guangdong Boxi Biotechnology) was added to each well of the PC group, and 2 mL of culture medium containing the corresponding concentration of the test sample was added to each well of the sample group. After drug administration, the 6-well plates were incubated in a CO2 incubator (37°C, 5% CO2) for 24 hours. After incubation, the old solution was discarded, and the cells were washed twice with PBS. 1 mL of RNAiso Plus (Aikerui Biotechnology Co., Ltd.) was added to each well. After cell lysis by pipetting, RNA was extracted, reverse transcribed into cDNA, and then detected by quantitative real-time PCR. The method is used to calculate the results.

[0107]

[0108] GraphPad Prism was used for plotting, and the results are expressed as Mean ± SD. t-tests were used for comparisons between groups. All statistical analyses were two-tailed. A p-value of 0.01 < p < 0.05 was considered statistically significant, and p < 0.01 was considered highly statistically significant. The mRNA amplification fold of group BC was normalized, and the significance of comparison with group BC was indicated by *, p < 0.05 by *, and p < 0.01 by **.

[0109] Table 7 Test Plan

[0110]

[0111] Note: Sample concentration 1 mg / mL (diluted with deionized water).

[0112] Table 8 Summary of Caspase-14 gene content detection results

[0113]

[0114]

[0115] (Note: ## indicates p < 0.01 compared to the blank control; ** indicates p < 0.01 compared to the blank control; * indicates p < 0.01 compared to the blank control.) <p<0.05。)

[0116] As can be seen from Table 8, the Caspase-14 gene content of the PC group increased significantly compared with the BC group, indicating that the positive control test was effective. Compared with the BC group, the single Cnidium monnieri extract did not have the effect of promoting Caspase-14 gene expression. Examples 1-3 and Comparative Examples 1, 2, 3, 5, 6, 7, 8 all had the effect of promoting Caspase-14 gene expression. This composition also had the function of synergistically promoting Caspase-14 gene expression. It can also be seen that the efficacy of KNYC① composition is stronger than that of KNYC② composition, and the synergistic promotion effect is also higher than that of KNYC② composition.

[0117] Table 9 LOR gene content detection result summary table

[0118]

[0119]

[0120] (Note: ## indicates p<0.01 compared with the blank control; ** indicates p<0.01 compared with the blank control; * indicates 0.01<p<0.05 compared with the blank control.)

[0121] As can be seen from Table 9, the LOR gene content of the PC group increased significantly compared with the BC group, indicating that the positive control test was effective. Compared with the BC group, the single Cnidium monnieri extract did not have the effect of promoting LOR gene expression. Examples 1-3 and Comparative Examples 1, 2, 3, 5, 6, 7, 8 all had the effect of promoting LOR gene expression. This composition also had the function of synergistically promoting LOR gene expression. It can also be seen that the efficacy of KNYC① composition is stronger than that of KNYC② composition, and the synergistic promotion effect is also higher than that of KNYC② composition.

[0122] Test Example 4: Human body moisturizing, firming and repairing efficacy evaluation

[0123] The human skin moisturizing, firming and repairing efficacy of Example 4 was tested, and a total of 36 people aged 30-55 years old who met the subject volunteer selection and exclusion criteria were tested.

[0124] Method of use: Cleanse the face in the morning and evening and apply an appropriate amount of Example 4 emulsion evenly on the face, gently massage until absorbed, twice a day, once in the morning and once in the evening, for 28 consecutive days.

[0125] Detection time: before use (D0), 14 days after use (D14), and 28 days after use (D28).

[0126] Test method:

[0127] 1. The VISIA-CR facial image analyzer is used to quantify the area contrast of the wrinkle (crow's feet, laugh lines, periorbital lines, glabellar lines, marionette lines, puppet wrinkle) as a parameter to assess the improvement of facial wrinkles.

[0128] 2. Cutometer MPA580 is used to detect skin elasticity and firmness. MPA580 is used to detect skin elasticity and firmness.

[0129] 3. Corneometer is used to detect the relative water content on the skin surface.

[0130] 4. Aqua Flux is used to detect the trans-epidermal water loss (TEWL) value of the skin to assess the skin barrier function.

[0131] Experimental parameters:

[0132] 1. Skin elasticity: The greater the R2, R5, R7 and Q1 values of skin elasticity, the better the skin elasticity. F4 is used to evaluate the skin firmness, the smaller the F4 value, the more firm the skin.

[0133] 2. Skin moisture content: The greater the skin moisture content value, the higher the skin moisture content.

[0134] 3. Trans-epidermal water loss (TEWL) value: The lower the TEWL value, the better the skin barrier.

[0135] Calculation formula:

[0136] SPSS Statistics 25 is used for statistical analysis, two-tailed test, test level α = 0.05.

[0137] According to the normal distribution test results, the difference analysis method is selected: if the measured value is normally distributed, the t-test method is used for statistical analysis; if it is not normally distributed, the rank sum test method is used for statistical analysis.

[0138] The test results are shown in Tables 10-18.

[0139] Table 10 Descriptive statistics results of wrinkle grade evaluation (n = 36)

[0140]

[0141]

[0142] Table 11 Statistical analysis results of wrinkle grade evaluation (n = 36)

[0143]

[0144] Statistical analysis results: " ": no statistically significant difference (P≥0.05); " ": statistically significant difference (0.01≤P<0.05); " ": statistically significant difference (0.001≤P<0.01); " ": statistically significant difference (P<0.001).

[0145] Table 12 Skin elasticity R2 descriptive statistics results (n=36)

[0146]

[0147] Statistical analysis results: " ": no statistically significant difference (P≥0.05); " ": statistically significant difference (0.01≤P<0.05); " ": statistically significant difference (0.001≤P<0.01); " ": statistically significant difference (P<0.001).

[0148] Table 13 Skin elasticity R5 content descriptive statistics results (n=36)

[0149]

[0150] Statistical analysis results: " ": no statistically significant difference (P≥0.05); " ": statistically significant difference (0.01≤P<0.05); " ": statistically significant difference (0.001≤P<0.01); " ": statistically significant difference (P<0.001).

[0151] Table 14 Skin elasticity R7 descriptive statistics results (n=36)

[0152]

[0153] Statistical analysis results: " ": no statistically significant difference (P≥0.05); " ": statistically significant difference (0.01≤P<0.05); " ": statistically significant difference (0.001≤P<0.01); " ": statistically significant difference (P<0.001).

[0154] Table 15 Skin elasticity Q1 descriptive statistics results (n=36)

[0155]

[0156] Statistical analysis results: "-" indicates no statistically significant difference (P≥0.05); "*" indicates statistically significant difference (0.01≤P<0.05); "**" indicates statistically significant difference (0.001≤P<0.01); "***" indicates statistically significant difference (P<0.001).

[0157] Table 16 Descriptive statistics of skin firmness F4 (n=36)

[0158]

[0159] Statistical analysis results: "-" indicates no statistically significant difference (P≥0.05); "*" indicates statistically significant difference (0.01≤P<0.05); "**" indicates statistically significant difference (0.001≤P<0.01); "***" indicates statistically significant difference (P<0.001).

[0160] Table 17 Descriptive statistics of skin moisture content (n=36)

[0161]

[0162] Statistical analysis results: "-" indicates no statistically significant difference (P≥0.05); "*" indicates statistically significant difference (0.01≤P<0.05); "**" indicates statistically significant difference (0.001≤P<0.01); "***" indicates statistically significant difference (P<0.001).

[0163] Table 18 Descriptive statistics of trans-epidermal water loss (TEWL) value (n=36)

[0164]

[0165] Statistical analysis results: "-" indicates no statistically significant difference (P≥0.05); "*" indicates statistically significant difference (0.01≤P<0.05); "**" indicates statistically significant difference (0.001≤P<0.01); "***" indicates statistically significant difference (P<0.001).

[0166] From Tables 10-18, it can be seen that, during the 4-week test period, Example 4 has significant efficacy in improving facial wrinkles and significant tightening efficacy in improving skin elasticity R2, R5, R7 and Q1, and the skin trans-epidermal water loss (TEWL) is significantly reduced, and the skin moisture content is significantly improved, so the essence cream containing the moisturizing and protecting tightening composition has good efficacy in improving facial wrinkles, relaxation, moisturizing and protection.

[0167] The above examples are only for illustrating the technical concept and characteristics of the present application, and the purpose is to enable those skilled in the art to understand the content of the present application and to implement it, and cannot limit the protection scope of the present application. Any equivalent changes or modifications made according to the spirit and essence of the present application shall be covered within the protection scope of the present application.

Claims

1. A moisturizing repair firming composition characterized in that, The moisturizing repair firming composition is made of the following components in mass fraction: 0.01-1.0 parts of sodium hyaluronate disaccharide, 1.0-15.0 parts of quinoa bran extract and artemisia capillaris extract composition, and 1.0-15.0 parts of radix peucedani extract; The preparation method of the quinoa bran extract and artemisia capillaris extract composition is as follows: S1, crude extraction: after the artemisia capillaris is dried, crushed and sieved, water is added to perform ultrasonic atomization extraction to obtain artemisia capillaris crude extract; The particle size of the sieved artemisia capillaris particles is between 50 mesh and 120 mesh, the mass ratio of the sieved artemisia capillaris particles to water is 1:6-10, and the ultrasonic atomization extraction temperature is controlled at 25-40℃, and the extraction time is 30-35 min; S2, deacidification: the artemisia capillaris crude extract obtained in step S1 is filtered, neutral aluminum oxide is added thereto, and the solid is removed by filtration to obtain artemisia capillaris extract; The mesh number of the neutral aluminum oxide is 80 mesh to 150 mesh; S3, adsorption: macroporous resin is used to perform adsorption treatment on the artemisia capillaris extract; The macroporous resin is any one or more of D101, AB-8 and HPD-100; The macroporous resin is placed in a separation column, and the separation column is a single column or a plurality of columns connected in series; S4, preparation of eluent: quinoa bran is mixed with butanediol, microwave heating low-temperature extraction is performed, the solid is removed by filtration, and the filtrate is diluted with water to obtain an eluent; The microwave heating extraction temperature is 45-55℃, and the extraction time is 90-100 min; S5, elution: the eluent obtained in step S4 is used to perform elution treatment on the macroporous resin to which the artemisia capillaris extract is adsorbed, which is obtained in step S3, to obtain refined extract; S6， Purification: the refined extract obtained in step S5 is filtered and purified to obtain the product; The preparation method of the radix peucedani extract is as follows: S1, the radix peucedani is crushed and sieved, then immersed in ethanol for ultrasonic extraction, filtered, and the filtrate is collected, and the filter residue is repeatedly extracted for 2-3 times, and the obtained filtrates are combined; S2, the filtrate obtained in step S1 is reduced pressure concentrated, ethanol is recovered to no alcohol taste, butanediol is added thereto, stirred uniformly, and filtered to obtain the product.

2. The moisturizing repair firming composition of claim 1, wherein The mesh number of the neutral aluminum oxide is 100 mesh.

3. The moisturizing repair firming composition of claim 1, wherein The macroporous resin is AB-8.

4. The moisturizing repair firming composition of claim 1 wherein, The moisturizing repair firming composition is made of the following components in mass fraction: 0.1-0.6 parts of sodium hyaluronate disaccharide, 5.0-10.0 parts of quinoa bran extract and artemisia capillaris extract composition, and 5.0-10.0 parts of radix peucedani extract.

5. The moisturizing repair firming composition of claim 1 wherein, In the preparation method of the radix peucedani extract, the sieving treatment in step S1 is performed by using a 30-60 mesh sieve; The ethanol is selected from 60%-85% ethanol, the solid-liquid ratio is 1:8-1:10 g / mL, the soaking time is controlled at 20-40 min, the ultrasonic power is 100-300 W during the ultrasonic extraction, and the ultrasonic treatment time is 40-60 min; In the preparation method, step S2 of reduced pressure concentration is as follows: reduced pressure distillation treatment is performed at 50-60℃, ethanol is recovered to no alcohol taste to obtain radix peucedani concentrated liquid, butanediol is added in an amount of 2-2.5 times the mass of the radix peucedani concentrated liquid, stirred uniformly, and filtered to obtain the product.

6. Use of the moisturizing repair firming composition according to any one of claims 1 to 5 for the preparation of a cosmetic product, characterized in that, The cosmetic has the effect of improving dryness, redness, sagging, and wrinkles of the skin.

7. A cosmetic for improving dryness, redness, laxity, wrinkles of the skin, characterized by, The cosmetic comprises the moisturizing and firming composition according to any one of claims 1 to 5.

8. The cosmetic product according to claim 7, wherein The cosmetic comprises other non-active ingredients allowed to be added in the cosmetic field.

9. The cosmetic product according to claim 8, wherein The cosmetic comprises an emulsifier, an emollient, a humectant, a thickening agent, and a preservative.

10. Process for the preparation of a cosmetic product according to any one of claims 7 to 9, characterized in that, The preparation method comprises the step of mixing the moisturizing and firming composition according to any one of claims 1 to 5 with other non-active ingredients.

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