Termite control composition and preparation method thereof

Abstract

The present application relates to the technical field of pest control, and specifically discloses a termite control composition and its preparation method. A kind of raw materials used in termite control composition include the following components in parts by weight: 0.9-1 parts of ivermectin emulsifiable concentrate; 1-1.2 parts of attractant; 0.5-0.6 parts of anti-photolysis agent; 4.8-5 parts of water; Its preparation method is: all raw materials are mixed evenly under ultrasonic conditions to obtain a termite control composition. The termite control composition of this application can be used for termite control work, with long-term stable and efficient prevention and control effect, and the degradation half-life under ultraviolet irradiation is not less than 4.21h. In the theoretical experiment of termite control within 2 hours, the average knockdown rate of termites can reach 95.96%, the average mortality rate can reach 82.83%, and the average missing weight ratio of wood blocks within 3 months is 9.42-9.43%.

Description

Technical Field

[0001] The present application relates to the technical field of pest control, and in particular to a termite control composition and a preparation method thereof. Background Art

[0002] The rampant spread of pests can have a significant negative impact on human life and cause serious damage to plants and crops. There are many types of pests, each threatening the growth of plants and crops in different ways. For example, leaf-feeding pests, such as the gnawing moth, white moth, and sawfly, will gnaw on the leaves of trees and lawns, weakening the trees. Piercing-sucking pests, such as aphids, psyllids, and scale insects, will suck plant sap and rob them of nutrients. In severe cases, this can cause the entire plant to wither or die, and can also induce sooty mold disease. Boring pests, such as longhorn beetles, wood wasps, and termites, will bore into tree branches, not only damaging the conductive tissues and causing plant death, but also forming crisscrossing tunnels within the wood, reducing the economic value of the wood. These borers, which live in seclusion, have few natural enemies, and are highly adaptable, making them extremely destructive and destructive.

[0003] Currently, efforts are underway to mitigate pest infestations through various methods, including strengthening plant quarantine, biological control, trunk injections, and pest trapping. Biological control, with its advantages of high lethality and long-lasting effectiveness, has become the most effective method of pest control. Fipronil is a phenylpyrazole insecticide that exhibits low termite repellency and high inactivation capacity at low concentrations, leading to its widespread use. However, fipronil is highly biotoxic and poses a serious threat to other animals in the ecosystem, such as butterflies and dragonflies. Consequently, researchers are attempting to replace fipronil with ivermectin, which also possesses highly effective insecticides. However, ivermectin is highly photodegradable and, after exposure to light in natural environments, struggles to maintain its original toxicity, preventing it from achieving long-term, stable termite control. Summary of the Invention

[0004] In order to effectively control termites in a long term, the present application provides a termite control composition and a preparation method thereof.

[0005] In a first aspect, the present application provides a termite control composition, the raw materials used comprising the following components in parts by weight: 0.9-1 parts of ivermectin emulsifiable concentrate; 1-1.2 parts of attractant; 0.5-0.6 parts of anti-photolysis agent; and 4.8-5 parts of water.

[0006] By adopting the above technical solution, the present application adds an anti-photolysis agent to the termite control composition, inhibiting the reaction process of the chain oxidation reaction of ivermectin in the ivermectin emulsifiable concentrate after ultraviolet irradiation in the natural environment, greatly reducing the possibility of destruction of the ivermectin molecular structure, thereby improving the anti-photolysis ability of the ivermectin emulsifiable concentrate, so that the termite control composition of the present application can achieve long-term and stable termite control effects. The present application also adds an attractant to attract termites to touch and gnaw on the termite control composition, assisting the ivermectin emulsifiable concentrate in inactivating termites, thereby achieving a more efficient termite control effect.

[0007] The present application also strictly controls the ratio between the various components, thereby optimizing the comprehensive performance of the termite control composition in controlling termites. The composition has good resistance to photolysis, and experimental data shows that the degradation half-life under ultraviolet irradiation is not less than 4.21 hours; the ability to knock down and inactivate termites is excellent, with an average termite knockdown rate of not less than 95.96% and an average termite mortality rate of not less than 82.83% in a 5-hour control simulation experiment; the termite control composition can effectively protect wood when applied in actual environments, with the average weight loss ratio of wood blocks within 3 months being not more than 9.43%.

[0008] Preferably, the weight ratio of the ivermectin emulsifiable concentrate, the attractant, the anti-photolysis agent and water is 1:1.12:0.55:5 in parts by weight.

[0009] By adopting the above technical solution, the present application further controls the ratio between the components and optimizes the control performance of the termite control composition to the optimal level. Experimental data show that when the weight ratio of ivermectin emulsifiable concentrate, attractant, anti-photolysis agent and water is 1:1.12:0.55:5, the comprehensive control performance of the termite control composition can reach the best state, the degradation half-life under ultraviolet irradiation is 4.22h, the average termite knockdown rate in a 5-hour control simulation experiment is 96.97%, the average termite mortality rate is 83.84%, and the average missing weight ratio of wood blocks within 3 months is as low as 9.21%.

[0010] Preferably, the anti-photolysis agent includes one or more of 2,6-di-tert-butyl-p-cresol, isooctyl salicylate and propyl gallate.

[0011] By adopting the above technical solution, the present application utilizes one or more of 2,6-di-tert-butyl-p-cresol, isooctyl salicylate, and propyl gallate as anti-photolysis agents, which can consume the free radicals necessary for the degradation reaction of ivermectin, significantly inhibiting the degradation process of ivermectin, thereby improving the stability of the control effect of the termite control composition. Moreover, the three anti-photolysis agents used in this application have stronger anti-photolysis capabilities than other anti-photolysis agents (such as benzotriazole UV absorbers), and can extend the ultraviolet photolysis half-life of the termite control composition of this application by at least 4.05 hours.

[0012] Preferably, the raw materials used also include spores of Metarhizium anisopliae at a concentration of 1×10 6 -1.02×10 6 The invention discloses a Metarhizium anisopliae dispersion having a concentration of 1 g / mL, wherein the weight ratio of the Metarhizium anisopliae dispersion to ivermectin emulsifiable concentrate is 1:(60-85).

[0013] Preferably, the Metarhizium anisopliae is any one of Metarhizium alba, Metarhizium luteum and Metarhizium spp.

[0014] By adopting the above technical solution, the present application also blends a certain spore number concentration of green muscardine dispersion with ivermectin emulsifiable concentrate in a certain weight ratio to obtain ivermectin emulsifiable concentrate with a stronger ability to inactivate termites. Among them, the present application utilizes white green muscardine, yellow green green muscardine and scarab green muscardine to induce termites to produce green muscardine disease, and form repeated infection within the termite population. In addition, ivermectin emulsifiable concentrate can greatly reduce the immune ability of termites, making termites more susceptible to infection by green muscardine. The infected termites will lose most of their ability to move and can no longer gnaw and destroy trees. Therefore, a good synergistic effect is produced between ivermectin emulsifiable concentrate and green muscardine, which can significantly improve the control performance of the termite control composition. At the same time, green muscardine will not pose a threat to human health and will hardly cause pollution to the environment. Its application in the termite control composition has high safety and practicality.

[0015] The present application also controls the weight ratio of the Metarhizium dispersion to the ivermectin emulsifiable concentrate, thereby further optimizing the control performance of the termite control composition. If the amount of ivermectin emulsifiable concentrate is too large, it will inhibit the ability of the bacterial flora in the Metarhizium dispersion to produce spores, thereby reducing the insecticidal ability of the Metarhizium dispersion; if the amount of ivermectin emulsifiable concentrate is too small, not only will the insecticidal ability of the entire termite control composition be reduced, but the effect of the ivermectin emulsifiable concentrate itself in reducing the immune ability of termites will also be greatly weakened.

[0016] Preferably, the Metarhizium is Metarhizium anisopliae.

[0017] By adopting the above-mentioned technical scheme, the present application further optimizes the species selection of Metarhizium anisopliae, gives full play to the synergistic effect of Metarhizium anisopliae and ivermectin emulsifiable concentrate, and experimental data proves that Metarhizium anisopliae can play a better role in assisting ivermectin in knocking down and inactivating termites compared with the two types of Metarhizium anisopliae, thereby increasing the average knockdown rate of termites by 2.1% within 5 hours and the average mortality rate by 3.49%.

[0018] Preferably, the weight ratio of the Metarhizium anisopliae dispersion to the ivermectin emulsifiable concentrate is 1:(70-80).

[0019] By adopting the above-mentioned technical solution, the present application further optimizes the weight ratio of green muscardine dispersion and ivermectin emulsifiable concentrate, and improves the comprehensive control ability of the termite control composition to the optimal level. Experimental data show that at this time, the average knockdown rate of termites within 5 hours can reach 98.99%, the average mortality rate can reach 92.93-93.94%, and the average missing weight ratio of wood blocks within 3 months can be as low as 7.01-7.21%.

[0020] Preferably, the attractant is one or more of Eucalyptus globulus bark, fir bark and sugarcane bagasse.

[0021] Preferably, the attractant is Eucalyptus globulus bark.

[0022] By adopting the above-mentioned technical solution, the present application utilizes Eucalyptus bark, fir bark, and sugarcane bagasse as attractants, increasing the likelihood that termites will touch and consume the termite control composition. The Eucalyptus bark and fir bark attract termites by triggering their memory of chewing wood, while the sugarcane bagasse induces termites to chew by emitting a sweet smell. Experiments have shown that, compared to the other two attractants, the use of Eucalyptus bark as an attractant in the present application can further increase the average knockdown rate and average mortality rate of termites, and reduce the average weight loss ratio of wood blocks over a three-month period.

[0023] In a second aspect, the present application provides a method for preparing a termite control composition, comprising the following steps: uniformly mixing all raw materials under ultrasonic conditions to obtain the termite control composition.

[0024] By adopting the above technical solution, the present application obtains a termite control composition with good comprehensive termite control ability after uniformly mixing all raw materials under ultrasonic conditions. The preparation method has easy-to-obtain raw materials, simple steps, and is convenient for industrial production, and has high practical use value.

[0025] In summary, this application has the following beneficial technical effects:

[0026] 1. The termite control composition of the present application has good resistance to photolysis, with a degradation half-life of not less than 4.21 hours under ultraviolet irradiation. It also has excellent termite knockdown and inactivation capabilities, with an average knockdown rate of not less than 95.96% and an average mortality rate of not less than 82.83% within 5 hours. When applied in practical environments, it can effectively protect wood, with an average wood block weight loss ratio of not more than 9.43% within 3 months.

[0027] 2. The preparation method of the termite control composition of the present application has readily available raw materials, simple steps, is convenient for industrial production, and has high practical use value. DETAILED DESCRIPTION

[0028] Material Source

[0029] Unless otherwise specified, the raw materials used in this application are all commercially available products, specifically:

[0030] Peptone, CAS No. 7896-98;

[0031] Sucrose was purchased from Xi'an Jinxiang Pharmaceutical Excipients Co., Ltd., with a residue on ignition of ≤0.1 wt%;

[0032] Tween-80 was purchased from Shandong Xufuyuan Chemical Co., Ltd., with an effective substance content of 99 wt%;

[0033] Ivermectin emulsifiable concentrate was purchased from Shunyi Co., Ltd., with an ivermectin content of 0.5 wt%;

[0034] 2,6-di-tert-butyl-p-cresol was purchased from Wuxi Chunhe Technology Co., Ltd., with an effective substance content of 99 wt%;

[0035] Isooctyl salicylate and propyl gallate were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., and the active substance content was 98 wt%;

[0036] Benzotriazole UV absorber UV-P, CAS No. 2440-22-4;

[0037] Benzotriazole UV absorber UV-1130, CAS No. 104810-48-2;

[0038] Benzotriazole UV absorber UV-328, CAS No. 25973-55-1;

[0039] White Metarhizium anisopliae, yellow-green Metarhizium anisopliae, and scarab Metarhizium anisopliae were provided by the Forest Protection Laboratory of the College of Forestry, Fujian Agriculture and Forestry University; Eucalyptus globulus bark and fir bark were provided by a local wood processing plant and were crushed, pulverized, and passed through a 200-mesh sieve.

[0040] Sugarcane bagasse was provided by a local food processing plant and was crushed, pulverized, and passed through a 200-mesh sieve.

[0041] The present application is further described in detail below with reference to preparation examples, embodiments and comparative examples.

[0042] Preparation Example 1.1

[0043] The preparation method of white Metarhizium anisopliae dispersion comprises the following steps: 10 g of peptone, 20 g of sucrose, 2 mL of Tween-80 and 2 kg of water are uniformly mixed to obtain a spore culture solution; then white Metarhizium anisopliae is placed in the spore culture solution to obtain a spore count concentration of 1×10 6 / mL of white Metarhizium anisopliae dispersion.

[0044] Preparation Example 1.2

[0045] The preparation method of white Metarhizium anisopliae dispersion comprises the following steps: 10 g of peptone, 20 g of sucrose, 2 mL of Tween-80 and 2 kg of water are uniformly mixed to obtain a spore culture solution; then white Metarhizium anisopliae is placed in the spore culture solution to obtain a spore count concentration of 1.02×10 6 / mL of white Metarhizium anisopliae dispersion.

[0046] Preparation Example 2

[0047] The preparation method of the yellow green Metarhizium anisopliae dispersion comprises the following steps: 10 g of peptone, 20 g of sucrose, 2 mL of Tween-80 and 2 kg of water are uniformly mixed to obtain a spore culture solution; then, the yellow green Metarhizium anisopliae is placed in the spore culture solution to obtain a spore count concentration of 1×10 6 / mL of yellow green Metarhizium anisopliae dispersion.

[0048] Preparation Example 3

[0049] The preparation method of the Metarhizium anisopliae dispersion comprises the following steps: 10 g of peptone, 20 g of sucrose, 2 mL of Tween-80 and 2 kg of water are uniformly mixed to obtain a spore culture solution; then, the Metarhizium anisopliae is placed in the spore culture solution to obtain a spore count concentration of 1×10 6 / mL of Metarhizium anisopliae dispersion.

[0050] Example 1.1

[0051] A method for producing a termite control composition comprises the following steps:

[0052] 9 kg of ivermectin emulsifiable concentrate, 12 kg of fir bark, 5 kg of benzotriazole ultraviolet absorber UV-P and 50 kg of water were treated under ultrasonic conditions for 25 minutes to obtain a termite control composition.

[0053] Example 1.2

[0054] A method for producing a termite control composition comprises the following steps:

[0055] 10 kg of ivermectin emulsifiable concentrate, 10 kg of fir bark, 6 kg of benzotriazole ultraviolet absorber UV-P and 48 kg of water were treated under ultrasonic conditions for 25 minutes to obtain a termite control composition.

[0056] Example 1.3

[0057] A method for producing a termite control composition comprises the following steps:

[0058] 10 kg of ivermectin emulsifiable concentrate, 11.2 kg of fir bark, 5.5 kg of benzotriazole ultraviolet absorber UV-P and 50 kg of water were treated under ultrasonic conditions for 25 minutes to obtain a termite control composition.

[0059] Example 1.4

[0060] A method for producing a termite control composition comprises the following steps:

[0061] 9.5 kg of ivermectin emulsifiable concentrate, 10.5 kg of fir bark, 5.9 kg of benzotriazole ultraviolet absorber UV-P and 49 kg of water were treated under ultrasonic conditions for 25 minutes to obtain a termite control composition.

[0062] Example 1.5

[0063] A method for producing a termite control composition comprises the following steps:

[0064] 9.2 kg of ivermectin emulsifiable concentrate, 11.8 kg of fir bark, 5.5 kg of benzotriazole ultraviolet absorber UV-P and 48.5 kg of water were treated under ultrasonic conditions for 25 minutes to obtain a termite control composition.

[0065] Examples 2.1-2.5

[0066] A method for preparing a termite control composition, which differs from Example 1.1 in that the benzotriazole ultraviolet absorber UV-P is replaced by benzotriazole ultraviolet absorber UV-P, benzotriazole ultraviolet absorber UV-1130, 2,6-di-tert-butyl-p-cresol, isooctyl salicylate, and propyl gallate, respectively; otherwise, the method is the same as Example 1.1.

[0067] Example 2.6

[0068] A method for preparing a termite control composition, which differs from Example 1.1 in that 5 kg of benzotriazole UV absorber UV-P is replaced by 2 kg of isooctyl salicylate and 3 kg of propyl gallate, and the rest is the same as Example 1.1.

[0069] Example 2.7

[0070] A method for preparing a termite control composition, which differs from Example 1.1 in that 5 kg of benzotriazole UV absorber UV-P is replaced with 2 kg of isooctyl salicylate and 3 kg of 2,6-di-tert-butyl-p-cresol, and the rest is the same as Example 1.1.

[0071] Example 2.8

[0072] A method for preparing a termite control composition, which differs from Example 1.1 in that 5 kg of benzotriazole ultraviolet absorber UV-P is replaced with 2 kg of 2,6-di-tert-butyl-p-cresol and 3 kg of propyl gallate, and the rest is the same as Example 1.1.

[0073] Example 2.9

[0074] A method for preparing a termite control composition, which differs from Example 1.1 in that 5 kg of benzotriazole UV absorber UV-P is replaced with 2 kg of isooctyl salicylate, 2 kg of 2,6-di-tert-butyl-p-cresol, and 1 kg of propyl gallate; otherwise, the method is the same as Example 1.1.

[0075] Example 3.1

[0076] A method for preparing a termite control composition, which differs from Example 1.1 in that 0.15 kg of the white metarhizium dispersion prepared in Preparation Example 1.1 is also added, and the rest is the same as Example 1.1.

[0077] Example 3.2

[0078] A method for preparing a termite control composition, which differs from Example 1.1 in that 0.106 kg of the white metarhizium dispersion prepared in Preparation Example 1.2 is also added, and the rest is the same as Example 1.1.

[0079] Example 4.1

[0080] A method for preparing a termite control composition, which differs from Example 3.1 in that the white Metarhizium anisopliae dispersion prepared in Preparation Example 1.1 is replaced by the yellow-green Metarhizium anisopliae dispersion prepared in Preparation Example 2, and the rest is the same as Example 3.1.

[0081] Example 4.2

[0082] A method for preparing a termite control composition, which differs from Example 3.1 in that the white Metarhizium anisopliae dispersion prepared in Preparation Example 1.1 is replaced by the Metarhizium anisopliae dispersion prepared in Preparation Example 3, and the rest is the same as Example 3.1.

[0083] Example 5.1

[0084] A method for preparing a termite control composition, which is different from Example 3.1 in that the amount of the white metarhizium dispersion prepared in Preparation Example 1.1 added is 0.106 kg, and the rest is the same as Example 3.1.

[0085] Example 5.2

[0086] A method for preparing a termite control composition, which is different from Example 3.1 in that the amount of the white metarhizium dispersion prepared in Preparation Example 1.1 added is 0.11 kg, and the rest is the same as Example 3.1.

[0087] Example 5.3

[0088] A method for preparing a termite control composition, which is different from Example 3.1 in that the amount of the white metarhizium dispersion prepared in Preparation Example 1.1 added is 0.1125 kg, and the rest is the same as Example 3.1.

[0089] Example 5.4

[0090] A method for preparing a termite control composition, which is different from Example 3.1 in that the amount of the white metarhizium dispersion prepared in Preparation Example 1.1 added is 0.12 kg, and the rest is the same as Example 3.1.

[0091] Example 5.5

[0092] A method for preparing a termite control composition, which is different from Example 3.1 in that the amount of the white metarhizium dispersion prepared in Preparation Example 1.1 added is 0.129 kg, and the rest is the same as Example 3.1.

[0093] Example 5.6

[0094] A method for preparing a termite control composition, which is different from Example 3.1 in that the amount of the white metarhizium dispersion prepared in Preparation Example 1.1 added is 0.14 kg, and the rest is the same as Example 3.1.

[0095] Example 5.7

[0096] A method for preparing a termite control composition, which differs from Example 1.1 in that 0.18 kg of the white metarhizium dispersion prepared in Preparation Example 1.1 is also added, and the rest is the same as Example 1.1.

[0097] Example 5.8

[0098] A method for preparing a termite control composition, which differs from Example 1.1 in that 0.09 kg of the white metarhizium dispersion prepared in Preparation Example 1.1 is also added, and the rest is the same as Example 1.1.

[0099] Example 6.1

[0100] A method for preparing a termite control composition, which differs from Example 1.1 in that the fir bark is replaced with eucalyptus bark, and the rest is the same as Example 1.1.

[0101] Example 6.2

[0102] A method for preparing a termite control composition, which differs from Example 1.1 in that fir bark is replaced with bagasse, and the rest is the same as Example 1.1.

[0103] Example 6.3

[0104] A method for preparing a termite control composition, which differs from Example 1.1 in that all the fir barks are replaced with 6 kg of Eucalyptus globulus bark and 6 kg of sugarcane bagasse, and the rest are the same as Example 1.1.

[0105] Example 6.4

[0106] A method for preparing a termite control composition, which differs from Example 1.1 in that all the fir barks are replaced with 6 kg of Eucalyptus globulus bark and 6 kg of fir bark, and the rest are the same as Example 1.1.

[0107] Example 6.5

[0108] A method for preparing a termite control composition, which differs from Example 1.1 in that all the fir bark is replaced with 6 kg of fir bark and 6 kg of sugarcane bagasse, and the rest is the same as Example 1.1.

[0109] Example 6.6

[0110] A method for preparing a termite control composition, which differs from Example 1.1 in that all the fir barks are replaced with 4 kg of Eucalyptus globulus bark, 4 kg of fir bark, and 4 kg of bagasse, and the rest are the same as Example 1.1.

[0111] Comparative Example 1

[0112] The difference from Example 1.1 is that the benzotriazole ultraviolet absorber UV-P is removed, and the rest is the same as Example 1.1.

[0113] Comparative Example 2

[0114] The difference from Example 1.1 is that the fir bark is removed, and the rest is the same as Example 1.1.

[0115] Comparative Example 3

[0116] The difference from Example 1.1 is that all the ivermectin emulsifiable concentrate is replaced with 0.3 kg of the Metarhizium anisopliae dispersion prepared in Preparation Example 3, and the rest is the same as Example 1.1.

[0117] Comparative Example 4.1

[0118] The difference from Example 1.1 is that the amount of ivermectin emulsifiable concentrate used is 8 kg, the amount of fir bark used is 15 kg, and the amount of benzotriazole ultraviolet absorber UV-P used is 2 kg. The rest is the same as Example 1.1.

[0119] Comparative Example 4.2

[0120] The difference from Example 1.1 is that the amount of ivermectin emulsifiable concentrate used is 12 kg, the amount of fir bark used is 8 kg, and the amount of benzotriazole ultraviolet absorber UV-P used is 8 kg. The rest is the same as Example 1.1.

[0121] Performance testing

[0122] 1. Anti-photolysis effect: Take 5 mL of each sample of the termite control composition prepared in Examples 1.1-6.2 and Comparative Examples 1-2, place them in a culture dish, and place them under a fluorescent lamp for illumination treatment. Sampling is taken regularly every 5 hours to measure the degradation rate. Sampling is stopped when the degradation rate reaches 90%. The half-life of each group is then calculated according to the degradation standard curve of the ivermectin concentration of the corresponding group / h, and are recorded in Table 1, where the illuminance during illumination is 80000 ± 20000 lx.

[0123] 2. Termite Control Effect (Theoretical): 30 mL of each of the termite control compositions prepared in Examples 1.1-6.2 and Comparative Examples 1-3.3 was evenly applied to a clean glass slide (25 cm × 25 cm) with a layer of moistened filter paper on the bottom. The temperature of the glass slide was controlled between 30°C and 35°C. Thirty termites were then transferred to the glass slide, which was then covered with a glass cover. Thirty-three termites were placed on each glass slide. After 2 hours, the number of termites knocked down and killed was recorded. This experiment was repeated three times, and the average knockdown rate and average mortality rate are recorded in Table 1.

[0124] 3. Termite Control Effect (Application): Pine wood blocks (50 mm × 50 mm × 10 mm) that had been kept in a constant temperature drying oven at (60±1)°C for 24 hours were completely immersed in the termite control compositions prepared in Examples 1.1-6.2 and Comparative Examples 1-3.3. Three replicates were set up for each group. After 10 minutes, the test blocks were removed and the surface liquid of the test blocks was gently blotted dry with filter paper. The soaked wood blocks were then naturally air-dried indoors to equilibrium moisture content. They were then randomly buried in forest soil. Three months after burial, the wood blocks were excavated and weighed. The average missing weight ratio (1%) of the wood blocks was calculated as follows and recorded in Table 1.

[0125] Table 1

[0126]

[0127]

[0128] Data Analysis:

[0129] As can be seen from Table 1, the termite control compositions of Examples 1.1-1.5 of the present application have a degradation half-life of not less than 4.21 hours under ultraviolet irradiation at an illuminance of 80,000 ± 20,000 lx. In a theoretical experiment of termite control within 2 hours, the average knockdown rate of termites can reach 95.96%, the average mortality rate can reach 82.83%, and the average weight loss ratio of wood blocks within 3 months is 9.42-9.43%. This proves that the addition of an anti-photolysis agent to the termite control composition of the present application can inhibit the reaction process of the chain oxidation reaction of ivermectin in the ivermectin emulsifiable concentrate after ultraviolet irradiation in the natural environment, greatly reducing the possibility of destruction of the molecular structure of ivermectin, thereby improving the anti-photolysis ability of the ivermectin emulsifiable concentrate. In addition, an attractant is added to attract termites to touch and gnaw on the termite control composition, so that the termite control composition of the present application can achieve a long-term and stable termite control effect.

[0130] The degradation half-life under ultraviolet irradiation of Example 1.3 was longer than that of Example 1.1 and Examples 1.4-1.5, the average termite knockdown rate and average mortality rate were both higher than those of Example 1.1 and Examples 1.4-1.5, and the average missing weight ratio of wood blocks was also lower than that of Example 1.1 and Examples 1.4-1.5. This demonstrates that the present application further optimizes the comprehensive performance of the termite control composition by further controlling the ratio of the raw materials in the composition.

[0131] The degradation half-life under ultraviolet irradiation of Examples 2.1-2.2 is basically the same as that of Example 1.1, and the degradation half-life under ultraviolet irradiation of Examples 2.3-2.9 is higher than that of Examples 1.1 and Examples 2.1-2.2. At the same time, the average loss ratio of wood blocks is much lower than that of Examples 1.1 and Examples 2.1-2.2, and the degradation half-life under ultraviolet irradiation of Example 2.5 can be as long as 13.49h, and the average loss ratio of wood blocks can be as low as 2.07%. It is proved that the present application uses one or more of 2,6-di-tert-butyl-p-cresol, isooctyl salicylate and propyl gallate as anti-photolysis agents, which can consume the free radicals necessary for the degradation reaction of ivermectin and greatly inhibit the degradation reaction process of ivermectin, thereby improving the stability of the control effect of the termite control composition, and the three anti-photolysis agents used in the present application are compared with other anti-photolysis agents (such as Benzotriazole ultraviolet absorber) has stronger anti-photolysis ability; the average loss ratio of wood blocks in Examples 3.1-4.2 is much lower than that in Example 1.1, and the average mortality rate of termites is higher than that in Example 1.1, which proves that the present application blends a green anisopliae dispersion with a certain spore number concentration and ivermectin emulsifiable concentrate in a certain weight ratio to obtain ivermectin emulsifiable concentrate with stronger termite inactivation ability, wherein the present application utilizes white green anisopliae, yellow green green anisopliae and scarab green anisopliae to induce termites to produce green anisopliae disease and form repeated infection in the termite population, and ivermectin emulsifiable concentrate can greatly reduce the immune ability of termites, making termites more susceptible to infection by green anisopliae, so a good synergistic effect is produced between ivermectin emulsifiable concentrate and green anisopliae, which can significantly improve the control performance of the termite control composition, and at the same time, green anisopliae will not pose a threat to human health and will hardly cause pollution to the environment;

[0132] The difference in Examples 5.1-5.8 is that the present application adjusts the amount of Metarhizium anisopliae dispersion added, and according to the experimental data, it can be concluded that the average knockdown rate and average mortality rate of termites in Examples 5.3-5.5 are the highest, and the average knockdown rate and average mortality rate of termites in Examples 5.7-5.8 are lower than those in other groups. This proves that the present application fully exerts the synergistic effect of Metarhizium anisopliae and ivermectin emulsifiable concentrate by strictly controlling the amount of Metarhizium anisopliae dispersion added, thereby further optimizing the control performance of the termite control composition. If the amount of ivermectin emulsifiable concentrate is too large, the ability of the bacterial community in the Metarhizium anisopliae dispersion to produce spores will be inhibited, thereby reducing the insecticidal ability of the Metarhizium anisopliae dispersion. If the amount of ivermectin emulsifiable concentrate is too small, not only will the insecticidal ability of the overall termite control composition be reduced, but the effect of ivermectin emulsifiable concentrate itself on reducing the immunity of termites will also be greatly weakened.

[0133] The average missing weight ratio of wood blocks in Example 6.1 was much lower than that in Example 1.1, and the average knockdown rate and average mortality rate of termites were also higher than those in Example 1.1. The various performances of Examples 6.2-6.6 were all on par with those in Example 1.1. This demonstrates that the use of Eucalyptus globulus bark as an attractant in this application can further improve the average knockdown rate and average mortality rate of termites, and reduce the average missing weight ratio of wood blocks within three months, compared to other attractants or compound attractants. Therefore, the use of Eucalyptus globulus bark in this application can optimize the comprehensive control performance of the termite control composition.

[0134] The degradation half-life of comparative example 1 under ultraviolet irradiation is much lower than that of example 1.1. At the same time, the average weight loss ratio of the wood blocks within 3 months is much higher than that of example 1.1, even as high as 20.96%, proving that the average weight loss ratio of the wood blocks of comparative example 2 of the present application is higher than that of example 1.1. At the same time, the average knockdown rate and average mortality rate of termites are also lower than those of example 1.1, proving that

[0135] The difference between Comparative Example 3 and Example 1.1 is that the present application adds twice the conventional amount of Metarhizium anisopliae dispersion instead of ivermectin emulsifiable concentrate with the same theoretical activity. Experimental data show that even if the amount of Metarhizium anisopliae dispersion, which has the best effect among the Metarhizium anisopliae dispersions, is increased, it cannot achieve the same rapid inactivation effect as ivermectin emulsifiable concentrate. However, according to the average missing weight ratio of wood blocks within 3 months, it can be seen that the Metarhizium anisopliae dispersion does have a certain termite control effect, but the average missing weight ratio of wood blocks is lower than that of Example 4.2, which proves that the ivermectin emulsifiable concentrate of the present application and Metarhizium anisopliae have a good synergistic effect, which can significantly improve the control performance of the termite control composition;

[0136] The average knockdown rate and average mortality rate of termites in Comparative Examples 4.1-4.2 were lower than those in Example 1.1, and the average missing weight ratio of wood blocks was much higher than that in Example 1.1, which proves that the present application can significantly optimize the comprehensive performance of the termite control composition for controlling termites by controlling the ratio of the various raw materials in the termite control composition.

[0137] The embodiments of this specific implementation method are all preferred embodiments of the present application and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A termite control composition, characterized in that: The raw materials used include the following components in parts by weight: 0.9-1 parts of ivermectin emulsifiable concentrate; 1-1.2 parts of an attractant; 0.5-0.6 parts of an anti-photolysis agent; and 4.8-5 parts of water. The anti-photolysis agent includes one or more of 2,6-di-tert-butyl-p-cresol, isooctyl salicylate, and propyl gallate. The raw materials used also include a spore count concentration of 1×10 6 -1.02×10 6 The invention discloses a Metarhizium anisopliae dispersion having a weight ratio of 1:(70-80) to the ivermectin emulsifiable concentrate of 1:3.0; the attractant is one or more of eucalyptus bark, fir bark and sugarcane bagasse.

2. The termite control composition according to claim 1, characterized in that: In parts by weight, the weight ratio of the ivermectin emulsifiable concentrate, the attractant, the anti-photolysis agent and water is 1:1.12:0.55:

5.

3. The termite control composition according to claim 1, characterized in that: The Metarhizium anisopliae is any one of Metarhizium alba, Metarhizium yellow-green and Metarhizium scarabe.

4. The termite control composition according to claim 3, characterized in that: The Metarhizium anisopliae is Metarhizium anisopliae.

5. The termite control composition according to claim 1, characterized in that: The attractant is the bark of Eucalyptus globulus.

6. A method for preparing the termite control composition according to any one of claims 1 to 5, characterized in that: The method comprises the following steps: uniformly mixing all raw materials under ultrasonic conditions to obtain a termite control composition.

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