A rapid healing dressing for mastectomy wounds and a preparation method thereof

Abstract

The invention discloses a rapid healing dressing for mastectomy wounds in the technical field of wound dressings and a preparation method thereof, comprising the following components in parts by weight: 20-30 parts of in-situ modified bacterial cellulose, 15-20 parts of thermosensitive polymer precursors, 0.35-0.5 parts of photoinitiators, and 35-50 parts of deionized water. The invention uses bacterial cellulose as the main supporting skeleton of the dressing, and uses a thermosensitive polymer precursor to form a winding structure. The thermosensitive polymer precursor has abundant N and O elements, and is fitted with the skin of the surgical incision by a multi-molecule force. After fitting the skin, the double bond structure in the thermosensitive monomer can quickly undergo polymerization reaction under light treatment conditions, thereby enhancing the fixing effect of the dressing on the wound, and enabling the dressing to have mechanical contraction through temperature response, thereby improving the toughness of the dressing and the speed of wound healing.

Description

Technical Field

[0001] The invention belongs to the technical field of wound dressings, and specifically refers to a dressing for rapid wound healing after mastectomy surgery and a preparation method thereof. Background Art

[0002] The breast is a glandular organ in the human body. Like the thyroid gland and pancreas, it is composed of glandular epithelial cells. When the morphology of the glandular epithelial cells changes, the breast function changes and breast hyperplasia occurs. Due to the change in the morphology of the glandular epithelial cells, the hyperplastic growth cannot be controlled, resulting in the formation of benign breast nodules. For benign breast nodules, the traditional small incision repair technology is generally used, which is simple to operate and has high repeatability. Traditional small incision surgery follows the steps of open surgery to find the mass, resulting in a relatively long operation time, with an average operation time of between 25-35 minutes, which is prone to postoperative complications, such as the formation of intramammary hematoma or intramammary bleeding. In addition, the size of the exposed wound caused by traditional small incision surgery is usually 2 -2.5cm, incision infection is also one of the common complications after surgery, which can easily cause tissue necrosis at the incision site and aggravate the infection; ideal medical dressings should have multiple functions such as protecting the wound, relieving inflammation and pain, promoting new granulation tissue and wound healing, etc. Surgical incision wounds are conventional acute wounds. If the wound can be accurately docked and good bandaging measures are incubated, the scar tissue produced will be very small, and the state of perfect repair can be achieved; usually the wound is docked by surgical suturing, which is easy to cause secondary damage to the wound and requires cumbersome operations such as removing stitches; in the prior art, conventional acute wounds are treated with wound closure dressings, but the strength and stability of the dressings are low, and they cannot quickly and firmly join the wound interface. Summary of the invention

[0003] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a dressing for rapid healing of mastectomy surgical wounds and a preparation method thereof. In order to solve the problem of poor mechanical properties of the dressing, the present invention uses bacterial cellulose as the main supporting skeleton of the dressing, and forms a winding structure on the skeleton with a thermosensitive polymer precursor. The thermosensitive polymer precursor has rich N and O elements, and is bonded to the skin of the surgical incision by multi-molecular forces. After bonding to the skin, the double bond structure in the thermosensitive monomer can quickly undergo polymerization reaction under light treatment conditions, thereby enhancing the dressing's fixation effect on the wound. The dressing can have mechanical contraction through temperature response, thereby improving the toughness of the dressing and increasing the speed of wound healing.

[0004] In order to achieve the above-mentioned object, the technical solution adopted by the present invention is as follows: The present invention provides a dressing for rapid wound healing after mastectomy, wherein the dressing comprises the following components in parts by weight:

[0005] 20-30 parts of in-situ modified bacterial cellulose, 15-20 parts of thermosensitive polymer precursor, 0.35-0.5 parts of photoinitiator, and 35-50 parts of deionized water;

[0006] Preferably, the photoinitiator comprises at least one of Irgacure-2959, Irgacure-184, Irgacure-651, Irgacure-369 and Irgacure-907;

[0007] Preferably, the viscosity of the rapid wound healing dressing is 60-70 Pa·s;

[0008] The present invention also provides a method for preparing a dressing for rapid wound healing in mastectomy surgery, which specifically comprises the following steps:

[0009] S1. Place N-isopropylacrylamide and diethanolamine in a flask, add anhydrous ethanol to dissolve, mix evenly, raise the temperature to 40-50° C. under a nitrogen atmosphere, and react for 2-3 hours to obtain an active monomer;

[0010] Preferably, in step S1, the mass ratio between the diethanolamine and N-isopropylacrylamide is 1:1-1.1;

[0011] Preferably, in step S1, after adding anhydrous ethanol, the mass concentration of N-isopropylacrylamide in anhydrous ethanol is 20-30 g / L;

[0012] S2, placing the active monomer and acrylate prepared in step S1 in a flask, adding dioxane to dissolve, stirring until the mixture is uniformly mixed, adding catalyst DBU under a nitrogen atmosphere, raising the reaction temperature to 80-90° C., reacting for 8-10 hours, washing, and drying to obtain a thermosensitive polymer precursor;

[0013] Preferably, in step S2, the acrylate comprises at least one of neopentyl glycol diacrylate, trimethylolpropane triacrylate and pentaerythritol tetraacrylate; and the mass ratio between the acrylate and the active monomer is 1:2.5-4.2;

[0014] Preferably, in step S2, the mass ratio between the catalyst DBU and the active monomer is 0.3-0.4:1;

[0015] S3, dissolving chloroauric acid in deionized water, heating to boiling, adding sodium citrate, continuing heating and stirring fully, when the solution turns red, cooling the reaction system to room temperature, adding thiol polyethylene glycol solution, reacting at 4°C for 24 hours, dialyzing with deionized water for 2 days, and freeze-drying to obtain polyethylene glycol-modified gold nanoparticles;

[0016] Preferably, in step S3, the mass concentration of chloroauric acid in deionized water is 0.08-0.1 g / L;

[0017] Preferably, in step S3, the mass ratio between the added mass of sodium citrate and chloroauric acid is 4-6:10;

[0018] Preferably, in step S3, in the mercapto polyethylene glycol solution, the mass concentration of mercapto polyethylene glycol in deionized water is 2-4 g / L; the added volume of the mercapto polyethylene glycol solution is 2%-4% of the volume of the chloroauric acid solution;

[0019] S4, inoculating the xylindracenacetobacter seed liquid into a fermentation medium, adding the polyethylene glycol-modified gold nanoparticles prepared in step S3 into the medium, culturing at 30° C. for 2-3 days, transferring to a newly configured fermentation medium and continuing to culture for 6-7 days, washing with a NaOH solution and deionized water, and freeze-drying to obtain in-situ modified bacterial cellulose:

[0020] Preferably, in step S4, the method for preparing the xylinum acetobacter seed solution is to pick xylinum acetobacter from the slant and inoculate it into 100 mL of seed culture medium, and culture it at 30° C. and 120 rpm for 18 hours to obtain the xylinum acetobacter seed solution; the ratio of the seed culture medium includes 10 g / L glucose and 10 g / L yeast powder;

[0021] Preferably, in step S4, the amount of the polyethylene glycol-modified gold nanoparticles added to the fermentation medium is 50-80 mg / L; the fermentation medium comprises 20 g / L glucose, 5 g / L yeast powder, 5 g / L peptone, 2.7 g / L disodium hydrogen phosphate, and 1.15 g / L citric acid;

[0022] Preferably, in step S4, the amount of the xylinacetobacter seed solution in the fermentation medium is 10-20%;

[0023] S5. Place the in-situ modified bacterial cellulose prepared in step S4 and the thermosensitive polymer precursor prepared in step S2 in a flask, add ethanol aqueous solution, raise the temperature to 70-80°C, stir at 400-500 rpm, stir for 12-14 hours, add a photoinitiator under light-proof conditions, remove ethanol, and add deionized water by weight to obtain a rapid wound healing dressing.

[0024] The beneficial effects achieved by the present invention are as follows:

[0025] The present invention provides a dressing for rapid healing of wounds in mastectomy surgery and a preparation method thereof. The present invention uses bacterial cellulose as the main supporting skeleton of the dressing, uses a thermosensitive polymer precursor to form a winding structure, and has rich N and O elements on the thermosensitive polymer precursor. The thermosensitive polymer precursor is attached to the skin of the surgical incision by a multi-molecule force. After attaching to the skin, the double bond structure in the thermosensitive monomer can quickly undergo polymerization reaction under light treatment conditions, thereby enhancing the fixing effect of the dressing on the wound. The dressing can have mechanical contraction through temperature response, thereby improving the toughness of the dressing and the speed of wound healing. The present invention uses N-isopropylacrylamide and diethanolamine double bond addition reaction, and then reacts with acrylates with different functionalities through Michael addition reaction to obtain a hyperbranched polymer monomer with polyamine thermosensitive groups, which is mixed with bacterial cellulose and a photoinitiator to form a The dressing gel can form a complex network structure with a temperature-responsive skeleton under light treatment conditions, thereby expanding the effect of temperature on the shrinkage of the dressing network structure. After being applied to the skin surface, the dressing changes at both ends of its phase change critical temperature, so that the dressing structure is tightened, the toughness of the dressing is increased, the fixation to the wound is increased, and the pulling effect of external force on the wound is reduced, so that the wound heals quickly in a stable environment. The present invention adopts an in-situ modification method to modify the bacterial cellulose, and by adding HS-PEG to the fermentation culture medium of Gluconobacter xylinum, the bacterial cellulose can be more evenly loaded with Au nanoparticles, thereby improving the antibacterial performance of the dressing, so as to avoid the infection of the wound and the occurrence of complications. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is the infrared spectrum of the thermosensitive polymer precursor prepared in step S2 of this embodiment;

[0027] Figure 2 This is a SEM image of the rapid wound healing dressing prepared in Example 1;

[0028] Figure 3 The water absorption results of the rapid wound healing dressings prepared in Examples 1-3 and Comparative Examples 1-3 are shown;

[0029] Figure 4 This is a graph showing the swelling rate results of the rapid wound healing dressings prepared in Examples 1-3 and Comparative Examples 1-3.

[0030] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0032] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0033] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials and test strains used in the following examples are purchased from commercial channels unless otherwise specified.

[0034] The present invention uses the xylinacetic acid bacteria ( Gluconacetobacter xylinus , formerly known as Acetobacter xylinum ) is deposited in China Center for Type Culture Collection (CCICC) with the deposit number CCTCC AB 2016224. Example 1

[0035] This embodiment provides a rapid wound healing dressing for mastectomy surgery, the rapid wound healing dressing comprising the following components in parts by weight: 20 parts of in-situ modified bacterial cellulose, 15 parts of thermosensitive polymer precursor, 0.4 parts of Irgacure-2959 photoinitiator, and 50 parts of deionized water;

[0036] This embodiment also provides a method for preparing a dressing for rapid wound healing in mastectomy surgery, which specifically comprises the following steps:

[0037] S1. Accurately weigh 1.13 g of N-isopropylacrylamide and 1.05 g of diethanolamine, place them in a three-necked flask, add 50 mL of anhydrous ethanol, dissolve N-isopropylacrylamide and diethanolamine by stirring, introduce nitrogen into the three-necked flask, increase the reaction temperature to 40° C., react for 3 hours, evaporate and remove anhydrous ethanol to obtain an active monomer;

[0038] S2, the active monomer prepared in step S1 was transferred to a four-necked flask, 9.0 g of pentaerythritol tetraacrylate was added, and then 30 mL of dioxane was added. After stirring and mixing, nitrogen was introduced into the four-necked flask to completely replace the oxygen in the reaction system, and then 0.6 mL of catalyst DBU was added dropwise, and the reaction temperature was increased to 85° C. After reacting for 9 hours, the dioxane in the reaction system was evaporated, and the organic phase was washed with 1 mol / L dilute hydrochloric acid solution after being redissolved with dichloromethane, and then washed with saturated NaCl solution, and then washed with MgSO 4 Drying to remove water, removing dichloromethane by rotary evaporator, and obtaining a thermosensitive polymer precursor after drying;

[0039] The thermosensitive polymer precursor prepared in step S2 was collected using a Nicilet iS10 infrared spectrometer and measured using the KBr pellet method with a scanning range of 4000-500 cm -1 , Figure 1 This is the infrared spectrum of the thermosensitive polymer precursor prepared in step S2 of this embodiment. As shown in the figure, at 1655-1640cm -1 Previously, the C=O bending vibration peak appeared at 1555-1540cm -1 The NH bond stretching vibration peak appeared between 1390-1360cm -1 There is an isopropyl -CH 3 The characteristic peaks of -CH- and -NH- indicate that the amide group in N-isopropylacrylamide still exists in this compound, 3400cm -1 The stretching vibration peak corresponding to -OH is 2970-2940cm -1 -CH appears between 2 - stretching vibration peak, proving that the thermosensitive polymer precursor was successfully synthesized in this example;

[0040] S3. Accurately weigh 1.0 g of chloroauric acid hydrate and dissolve it in 100 mL of deionized water. Take 1 mL and transfer it to 100 mL of deionized water to prepare a chloroauric acid solution. Prepare sodium citrate into a 1 wt% aqueous solution. Heat the chloroauric acid solution to boiling, add 4 mL of sodium citrate solution dropwise, continue heating and stir thoroughly. When the reaction system turns red, cool it to room temperature, add 2 mL of 2 g / L mercapto polyethylene glycol solution to the reaction system, place it at 4 ° C for 24 hours, and dialyze it with deionized water using a dialysis bag with a cutoff of 5000D for 2 days. After removing unreacted mercapto polyethylene glycol, freeze-dry it to obtain polyethylene glycol-modified gold nanoparticles.

[0041] S4, accurately weigh 1g glucose and 1g yeast powder and dissolve them in deionized water, and sterilize them at 121℃ for 2h to prepare seed culture medium. After the seed culture medium is cooled, place the xylindracenacetobacter picked from the slant in the seed culture medium, and shake and culture at 30℃ and 120rpm for 18h to obtain xylindracenacetobacter seed solution; according to 20g / L glucose, 5g / L yeast powder, 5g / L peptone, 2.7g / L disodium hydrogen phosphate, 1.15 g / L citric acid, 50mg / L polyethylene glycol-modified gold nanoparticles were used to prepare a fermentation medium, which was sterilized at 121°C for 2h. After the fermentation medium was cooled to room temperature, 1mL of xylindoleacetobacterium seed liquid was inoculated into 9mL of fermentation medium, and cultured at 30°C and 120rpm for 2d. The medium was then transferred to 90mL of a newly prepared fermentation medium, and cultured at 30°C for 7d. The medium was sterilized at 75°C, washed repeatedly three times with 1mol / L NaOH solution and deionized water, and freeze-dried to obtain in situ modified bacterial cellulose.

[0042] S5. Place the in situ modified bacterial cellulose prepared in step S4 and the thermosensitive polymer precursor prepared in step S2 in a flask, add 50 mL of ethanol aqueous solution, raise the temperature to 70°C, stir at 500 rpm, and after stirring for 14 hours, add Irgacure-2959 photoinitiator under light-proof conditions, evaporate and remove ethanol, and add deionized water in parts by weight to obtain a rapid wound healing dressing, the viscosity of which is 68.83 Pa·s. Example 2

[0043] This embodiment provides a rapid wound healing dressing for mastectomy surgery, the rapid wound healing dressing comprising the following components in parts by weight: 30 parts of in-situ modified bacterial cellulose, 15 parts of thermosensitive polymer precursor, 0.4 parts of Irgacure-184 photoinitiator, and 45 parts of deionized water;

[0044] This embodiment also provides a method for preparing a dressing for rapid wound healing in mastectomy surgery, which specifically comprises the following steps:

[0045] S1. Accurately weigh 2.3 g of N-isopropylacrylamide and 2.1 g of diethanolamine, place them in a three-necked flask, add 80 mL of anhydrous ethanol, dissolve N-isopropylacrylamide and diethanolamine by stirring, introduce nitrogen into the three-necked flask, increase the reaction temperature to 50° C., react for 2 h, evaporate and remove anhydrous ethanol to obtain an active monomer;

[0046] S2. The active monomer prepared in step S1 was transferred to a four-necked flask, 11.0 g of neopentyl glycol diacrylate and 30 mL of dioxane were added, and after stirring and mixing, nitrogen was introduced into the four-necked flask to completely replace the oxygen in the reaction system, and 0.8 mL of catalyst DBU was added dropwise. The reaction temperature was raised to 80° C. After reacting for 10 h, the dioxane in the reaction system was evaporated and redissolved with dichloromethane. The organic phase was washed with 1 mol / L dilute hydrochloric acid solution, washed with saturated NaCl solution, and then washed with MgSO 4 Drying to remove water, removing dichloromethane by rotary evaporator, and obtaining a thermosensitive polymer precursor after drying;

[0047] S3, accurately weigh 0.8g of chloroauric acid hydrate and dissolve it in 100mL of deionized water, take 1mL and transfer it to 100mL of deionized water to prepare chloroauric acid solution, prepare sodium citrate into a 1wt% aqueous solution, heat the chloroauric acid solution to boiling, add 4.8mL of sodium citrate solution dropwise, continue heating and stir thoroughly, when the reaction system turns red, cool to room temperature, add 2mL of 4g / L mercapto polyethylene glycol solution to the reaction system, place it at 4°C for reaction for 24h, dialyze with deionized water using a dialysis bag with a cutoff of 5000D, the dialysis time is 3d, remove unreacted mercapto polyethylene glycol, and then freeze-dry to obtain polyethylene glycol-modified gold nanoparticles;

[0048] S4, accurately weigh 1g glucose and 1g yeast powder and dissolve them in deionized water, and sterilize them at 121℃ for 2h to prepare seed culture medium. After the seed culture medium is cooled, place the xylindracenacetobacter picked from the slant in the seed culture medium, and shake and culture at 30℃ and 120rpm for 18h to obtain xylindracenacetobacter seed solution; according to 20g / L glucose, 5g / L yeast powder, 5g / L peptone, 2.7g / L disodium hydrogen phosphate, 1.15 g / L citric acid, 60mg / L polyethylene glycol-modified gold nanoparticles were used to prepare a fermentation medium, which was sterilized at 121°C for 2h. After the fermentation medium was cooled to room temperature, 2mL of xylindoleacetobacter seed liquid was inoculated into 8mL of fermentation medium, and cultured at 30°C and 120rpm for 2d. The medium was then transferred to 90mL of a newly prepared fermentation medium, cultured at 30°C for 6d, and sterilized at 75°C. The medium was washed repeatedly three times with 1mol / L NaOH solution and deionized water, and freeze-dried to obtain in situ modified bacterial cellulose.

[0049] S5. Place the in-situ modified bacterial cellulose prepared in step S4 and the thermosensitive polymer precursor prepared in step S2 in a flask, add 50 mL of ethanol aqueous solution, raise the temperature to 80°C, stir at 400 rpm, and after stirring for 12 hours, add Irgacure-2959 photoinitiator under light-proof conditions, evaporate and remove ethanol, and add deionized water by weight to obtain a rapid wound healing dressing having a viscosity of 65.35 Pa•s. Example 3

[0050] This embodiment provides a rapid wound healing dressing for mastectomy surgery, the rapid wound healing dressing comprising the following components in parts by weight: 20 parts of in-situ modified bacterial cellulose, 20 parts of thermosensitive polymer precursor, 0.4 parts of Irgacure-2959 photoinitiator, and 35 parts of deionized water;

[0051] This embodiment also provides a method for preparing a dressing for rapid wound healing in mastectomy surgery, which specifically comprises the following steps:

[0052] S1. Accurately weigh 1.5 g of N-isopropylacrylamide and 1.5 g of diethanolamine, place them in a three-necked flask, add 50 mL of anhydrous ethanol, dissolve N-isopropylacrylamide and diethanolamine by stirring, introduce nitrogen into the three-necked flask, increase the reaction temperature to 45° C., react for 3 h, evaporate and remove anhydrous ethanol to obtain an active monomer;

[0053] S2, the active monomer prepared in step S1 was transferred to a four-necked flask, 10.5 g of trimethylolpropane triacrylate was added, and then 30 mL of dioxane was added. After stirring and mixing, nitrogen was introduced into the four-necked flask to completely replace the oxygen in the reaction system, and then 1.2 mL of catalyst DBU was added dropwise, and the reaction temperature was increased to 90°C. After reacting for 8 hours, the dioxane in the reaction system was evaporated, and the organic phase was washed with 1 mol / L dilute hydrochloric acid solution after being redissolved with dichloromethane, and then washed with saturated NaCl solution, and then washed with MgSO 4 Drying to remove water, removing dichloromethane by rotary evaporator, and obtaining a thermosensitive polymer precursor after drying;

[0054] S3, accurately weigh 0.9g of chloroauric acid hydrate and dissolve it in 100mL of deionized water, take 1mL and transfer it to 100mL of deionized water to prepare chloroauric acid solution, prepare sodium citrate into a 1wt% aqueous solution, heat the chloroauric acid solution to boiling, add 4.5mL of sodium citrate solution dropwise, continue heating and stir thoroughly, when the reaction system turns red, cool to room temperature, add 4mL of 3g / L mercapto polyethylene glycol solution to the reaction system, place it at 4℃ for reaction for 24h, dialyze with deionized water using a dialysis bag with a cutoff of 5000D, the dialysis time is 2d, remove unreacted mercapto polyethylene glycol, and then freeze-dry to obtain polyethylene glycol-modified gold nanoparticles;

[0055] S4, accurately weigh 1g glucose and 1g yeast powder and dissolve them in deionized water, and sterilize them at 121℃ for 2h to prepare seed culture medium. After the seed culture medium is cooled, place the xylindracenacetobacter picked from the slant in the seed culture medium, and shake and culture at 30℃ and 120rpm for 18h to obtain xylindracenacetobacter seed solution; according to 20g / L glucose, 5g / L yeast powder, 5g / L peptone, 2.7g / L disodium hydrogen phosphate, 1.15 g / L citric acid, 80mg / L polyethylene glycol-modified gold nanoparticles were used to prepare a fermentation medium, which was sterilized at 121°C for 2h. After the fermentation medium was cooled to room temperature, 1mL of xylindoleacetobacterium seed liquid was inoculated into 9mL of fermentation medium, and cultured at 30°C and 120rpm for 3d. The medium was then transferred to 90mL of a newly prepared fermentation medium, and cultured at 30°C for 7d. The medium was sterilized at 75°C, washed repeatedly three times with 1mol / L NaOH solution and deionized water, and freeze-dried to obtain in situ modified bacterial cellulose.

[0056] S5. Place the in-situ modified bacterial cellulose prepared in step S4 and the thermosensitive polymer precursor prepared in step S2 in a flask, add 50 mL of ethanol aqueous solution, raise the temperature to 750°C, stir at 500 rpm, and after stirring for 13 hours, add Irgacure-2959 photoinitiator under light-proof conditions, evaporate and remove ethanol, and add deionized water by weight to obtain a rapid wound healing dressing having a viscosity of 61.53 Pa•s.

[0057] Comparative Example 1

[0058] This comparative example provides a wound dressing and a preparation method thereof, which is different from Example 1 only in that the dressing does not include a thermosensitive polymer precursor and a photoinitiator, and the remaining components and component contents are the same as those in Example 1.

[0059] Comparative Example 2

[0060] This comparative example provides a wound dressing and a preparation method thereof. The difference from the remaining embodiment 1 is that, in the preparation method, step S1 is not included, the active monomer in step S2 is replaced by diethanolamine of the same mass to obtain a polymer precursor, and subsequent reactions are carried out. The remaining technical schemes are the same as those in embodiment 1.

[0061] Comparative Example 3

[0062] This comparative example provides a wound dressing and a preparation method thereof. The only difference from the remaining embodiment 1 is that the preparation method does not include step S3, the fermentation medium in step S4 does not contain thiol-polyethylene glycol-modified gold nanoparticles, and after the bacterial cellulose is prepared, it is mixed with gold nanoparticles to form modified bacterial fiber, and then the subsequent preparation is carried out. The remaining technical schemes are the same as those in embodiment 1.

[0063] Experimental Example 1

[0064] In this experimental example, a scanning electron microscope was used to observe the microscopic morphology of the rapid wound healing dressing prepared in Example 1. After the rapid wound healing dressing prepared in Example 1 was freeze-dried, a 460-type scanning electron microscope was used to observe the microstructure. Figure 2 This is a SEM image of the rapid wound healing dressing prepared in Example 1. As shown in the figure, the rapid wound healing dressing prepared in Example 1 has a dense three-dimensional network structure.

[0065] Experimental Example 2

[0066] In this experimental example, the water absorption and swelling properties of the rapid wound healing dressings prepared in Examples 1-3 and Comparative Examples 1-3 were measured. 0.1 g of the rapid wound healing dressing samples prepared in the Examples and Comparative Examples were placed on a glass plate, and the weight was recorded as W0. 5 mL of PBS buffer was added, and the measurement was performed every 1 min. The PBS buffer was appropriately supplemented according to the water absorption, and the sample weight Wt at t was recorded. The water absorption rate (%) was calculated according to the following formula:

[0067] Water absorption (%) = (Wt-W0) / W0×100%;

[0068] Weigh 0.1 g of the rapid wound healing dressing samples prepared in Example and Comparative Example 1, measure the initial volume as V0, place the samples in a 12-well plate containing a sufficient amount of PBS buffer, and soak in a 37° C. constant temperature incubator. Measure the volume Vt of the samples every 1 min, and calculate the swelling rate according to the following formula;

[0069] Swelling rate (%) = (Vt-V0) / V0×100%.

[0070] Figure 3 This is a graph showing the water absorption results of the rapid wound healing dressings prepared in Examples 1-3 and Comparative Examples 1-3. Figure 4 The swelling rate result graph of the rapid wound healing dressings prepared by Examples 1-3 and Comparative Examples 1-3 is shown in the figure. As far as the water absorption rate is concerned, the absorption capacity of the PBS buffer solution by each treatment reaches more than 300%, wherein the water absorption rate of the rapid wound healing dressing described in the example is more than 400%; from the swelling rate point of view, the swelling rate of the rapid wound healing dressing prepared by the example remains in a relatively stable state, and when the water absorption reaches more than 400%, it can maintain a good structure, achieve adhesion at the wound interface, and ensure that the mechanical properties of the dressing due to water absorption decrease; the wound dressing prepared by Comparative Example 2 can reach the maximum swelling volume in about 2 minutes, and can maintain the physical state of the dressing. The physical structure of Comparative Examples 1 and 2 collapses during the swelling process.

[0071] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0072] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A method for preparing a dressing for rapid wound healing in mastectomy surgery, characterized in that: The specific steps include: S1. Place N-isopropylacrylamide and diethanolamine in a flask, add anhydrous ethanol to dissolve, mix evenly, raise the temperature to 40-50° C. under a nitrogen atmosphere, and react for 2-3 hours to obtain an active monomer; S2, placing the active monomer and acrylate prepared in step S1 in a flask, adding dioxane to dissolve, stirring until the mixture is uniformly mixed, adding catalyst DBU under a nitrogen atmosphere, raising the reaction temperature to 80-90° C., reacting for 8-10 hours, washing, and drying to obtain a thermosensitive polymer precursor; S3, dissolving chloroauric acid in deionized water, heating to boiling, adding sodium citrate, continuing heating and stirring fully, when the solution turns red, cooling the reaction system to room temperature, adding thiol polyethylene glycol solution, reacting at 4°C for 24 hours, dialyzing with deionized water for 2 days, and freeze-drying to obtain polyethylene glycol-modified gold nanoparticles; S4, inoculating the xylindracenacetobacter seed liquid into a fermentation medium, adding the polyethylene glycol-modified gold nanoparticles prepared in step S3 into the medium, culturing at 30° C. for 2-3 days, transferring to a newly configured fermentation medium and continuing to culture for 6-7 days, washing with a NaOH solution and deionized water, and freeze-drying to obtain in-situ modified bacterial cellulose: S5. Place the in-situ modified bacterial cellulose prepared in step S4 and the thermosensitive polymer precursor prepared in step S2 in a flask, add ethanol aqueous solution, raise the temperature to 70-80°C, stir at 400-500 rpm, stir for 12-14 hours, add a photoinitiator under light-proof conditions, remove ethanol, and add deionized water by weight to obtain a rapid wound healing dressing.

2. The method for preparing a rapid healing dressing for mastectomy wounds according to claim 1, characterized in that: In step S1, the mass ratio of diethanolamine to N-isopropylacrylamide is 1:1-1.1; after adding anhydrous ethanol, the mass concentration of N-isopropylacrylamide in anhydrous ethanol is 20-30 g / L.

3. The method for preparing a rapid healing dressing for mastectomy wounds according to claim 2, characterized in that: In step S2, the acrylate includes at least one of neopentyl glycol diacrylate, trimethylolpropane triacrylate and pentaerythritol tetraacrylate; the mass ratio of the acrylate to the active monomer is 1:2.5-4.2; and the mass ratio of the catalyst DBU to the active monomer is 0.3-0.4:

1.

4. The method for preparing a rapid healing dressing for mastectomy wounds according to claim 3, characterized in that: In step S3, the mass concentration of the chloroauric acid in deionized water is 0.08-0.1 g / L; the mass ratio between the added mass of the sodium citrate and the chloroauric acid is 4-6:

10.

5. The method for preparing a dressing for rapid wound healing in mastectomy surgery according to claim 4, characterized in that: In step S3, in the mercapto polyethylene glycol solution, the mass concentration of mercapto polyethylene glycol in deionized water is 2-4 g / L; the added volume of the mercapto polyethylene glycol solution is 2%-4% of the volume of the chloroauric acid solution.

6. The method for preparing a dressing for rapid wound healing in mastectomy surgery according to claim 5, characterized in that: In step S4, the method for preparing the xylindracenacetobacter seed liquid is as follows: xylindracenacetobacter is selected from the slant and inoculated into 100 mL of seed culture medium, and then cultured at 30° C. and 120 rpm for 18 hours to obtain xylindracenacetobacter seed liquid; the ratio of the seed culture medium includes 10 g / L glucose and 10 g / L yeast powder.

7. The method for preparing a dressing for rapid wound healing in mastectomy surgery according to claim 6, characterized in that: In step S4, the amount of the polyethylene glycol-modified gold nanoparticles added to the fermentation medium is 50-80 mg / L; the ratio of the fermentation medium includes 20 g / L glucose, 5 g / L yeast powder, 5 g / L peptone, 2.7 g / L disodium hydrogen phosphate, and 1.15 g / L citric acid; the accession amount of the xylinacetobacter seed liquid in the fermentation medium is 10-20%.

8. A dressing for rapid wound healing in mastectomy surgery prepared according to the preparation method according to any one of claims 1 to 7, characterized in that: The rapid wound healing dressing comprises the following components in parts by weight: 20-30 parts of in-situ modified bacterial cellulose, 15-20 parts of thermosensitive polymer precursor, 0.35-0.5 parts of photoinitiator, and 35-50 parts of deionized water.

9. The rapid healing dressing for mastectomy wounds according to claim 8, characterized in that: The photoinitiator includes at least one of Irgacure-2959, Irgacure-184, Irgacure-651, Irgacure-369 and Irgacure-907.

10. The rapid healing dressing for mastectomy wounds according to claim 9, characterized in that: The viscosity of the rapid wound healing dressing is 60-70 Pa•s.

Images