Production method and application of an organic biological fertilizer for green seedlings and tail vegetables

Abstract

The present invention discloses a production method and application of an organic biological fertilizer from green seedlings and tail vegetables, belonging to the field of agricultural production. After the green seedlings and tail vegetables are crushed, they are mixed with yellow mealworm feces and Lactobacillus plantarum, and anaerobically fermented to obtain fermentation product I; actinomycetes are added to the fermentation product I, and intermittent stirring is carried out for continuous fermentation culture, and the obtained fermentation product II is the organic biological fertilizer from green seedlings and tail vegetables. It is found through experimental verification that the organic biological fertilizer prepared by the present invention can significantly increase the yield of potatoes and the starch content of tubers.

Description

Technical Field

[0001] The present invention relates to the field of agricultural production, and particularly to a production method and application of an organic bio-fertilizer for green seedlings and tail vegetables. Background Art

[0002] Chinese cabbage is a kind of vegetable mainly planted in the north. It was previously planted in summer and harvested in winter. However, with the progress of science and technology, it can be planted throughout the year to provide fresh vegetables for consumers. However, when planting Chinese cabbage, it is generally sown and transplanted. During the seedling stage or the transplanting process, in order to ensure the survival rate of transplanted Chinese cabbage, multiple seedlings are usually transplanted, and in the later stage, the seedlings are removed, and only the seedlings with better growth conditions (commonly known as "green seedlings") are retained. The excess seedlings are generally directly discarded. After the Chinese cabbage matures, it is harvested, transported, processed, etc. During this process, in order to ensure a long storage period and meet the consumers' requirements for appearance, the outer dry leaves, rotten, yellowed and other bad leaves are removed, thus generating a large amount of tail vegetables that cannot be directly eaten. Whether it is green seedlings or tail vegetables, they are essentially Chinese cabbage, and like Chinese cabbage, they contain nutritional components such as dietary fiber and amino acids. For green seedlings or tail vegetables, at present, most of them are directly discarded or used to produce biogas. However, direct discarding will pollute the environment, and the input cost of producing biogas is higher than the output cost, resulting in an unprofitable situation, so that the treatment of green seedlings and tail vegetables cannot be effectively solved fundamentally. In addition, many existing literature and patents report that some wastes such as straw, tail vegetables, and strains are mixed and fermented to prepare bio-fertilizers to improve their application value. However, on the one hand, the raw material preparation is complex and diverse, and on the other hand, using strains for fermentation, due to the differences between strains, the utilization of raw materials is uneven, which in turn leads to limited efficacy and utilization value of the organic fertilizer, and also restricts the reasonable and effective utilization of wastes and other problems.

[0003] Potatoes have multiple characteristics such as being both food, vegetable and fruit, and are one of the important food varieties in many countries in the world. Potatoes contain a large amount of starch, which, as an important food additive, plays an important role in the food and beverage industries. It is not only used as a thickener and stabilizer, but also plays an important role in the production of many processed foods, such as soups, sauces and gravies. In addition, potato starch is also used in industries such as baking raw materials, frozen desserts, and biomedicine to meet consumers' needs for healthy foods and functional foods. It can be seen that the added value of potato starch is very high. However, the starch content in ordinary potatoes is relatively low, and it takes a long time to cultivate potatoes with high starch content. Therefore, how to improve the starch content of existing potato varieties has been a project that researchers have been concerned about and developing. Traditional reasonable fertilization can promote the increase of potato starch content. However, at present, ordinary bio-fertilizers or chemical fertilizers have a certain effect on potato yield, but almost no effect on the increase of potato starch content.

[0004] At present, there is no report on the use of solely green seedlings and tail vegetables as the basic substrates for fermenting and producing organic bio-fertilizers to increase the starch content of potatoes. Summary of the Invention

[0005] The purpose of the present invention is to provide a method for producing an organic bio-fertilizer from green seedlings and tail vegetables, so as to solve the problems existing in the above-mentioned prior art. The organic bio-fertilizer produced by this method can significantly increase the potato yield and the starch content of tubers.

[0006] To achieve the above purpose, the present invention provides the following solutions:

[0007] The present invention provides a method for producing an organic bio-fertilizer from green seedlings and tail vegetables, including the following steps:

[0008] After crushing the green seedlings and tail vegetables, mix them with yellow mealworm feces and Lactobacillus plantarum, and perform anaerobic fermentation to obtain fermentation product I;

[0009] Add actinomycetes to the fermentation product I, and continue fermentation culture with intermittent stirring. The obtained fermentation product II is the organic bio-fertilizer of green seedlings and tail vegetables.

[0010] Preferably, the mass ratio of the green seedlings and tail vegetables, yellow mealworm feces, and Lactobacillus plantarum is (3 - 5):(1 - 2):0.5, and the green seedlings and the tail vegetables are mixed in a mass ratio of 1:1.

[0011] Preferably, the conditions for the anaerobic fermentation culture are: fermentation culture at 32°C for 10 - 15 h.

[0012] Preferably, the mass ratio of the fermentation product I to the actinomycetes is (4 - 5):2.

[0013] Preferably, the intermittent stirring is carried out every 1 h and stirred for 20 min under the condition of 70 - 80 rpm.

[0014] Preferably, the conditions for the continued fermentation culture are: fermentation culture at 30°C for 24 - 48 h.

[0015] Preferably, the Lactobacillus plantarum is Lactobacillus plantarum HM - 20, and the preservation number is CGMCC No.6744; the actinomycetes is actinomycetes SCAUT009, and the preservation number is GDMCC No:61508.

[0016] The present invention also provides an organic bio-fertilizer of green seedlings and tail vegetables, which is prepared by the above production method.

[0017] The present invention also provides the application of the organic bio-fertilizer of green seedlings and tail vegetables in increasing the potato yield.

[0018] The present invention also provides the application of the above-mentioned green rice seedlings and vegetable tail organic bio-fertilizer in increasing the starch content of potatoes.

[0019] The present invention discloses the following technical effects:

[0020] In the present invention, based on Chinese cabbage green rice seedlings and vegetable tails as the basic fermentation substrates, adding Lactobacillus plantarum and yellow mealworm feces can fully release the nutrient components in the green rice seedlings and vegetable tails. At the same time, the yellow mealworm feces can provide sufficient nutrients for Lactobacillus plantarum to promote the growth and reproduction of Lactobacillus plantarum, making Lactobacillus plantarum become the dominant strain. Since the nutrients contained in green rice seedlings, vegetable tails and yellow mealworm feces are difficult to be fully decomposed and utilized by a single strain, based on this, actinomycetes are added to the fermentation products produced by Lactobacillus plantarum fermentation. Because Lactobacillus plantarum can produce various enzymes, small molecule nutrients, etc., under the initial acidic environmental conditions, the growth of actinomycetes is slow, but sufficient nutrients and intermittent stirring can provide advantageous fermentation conditions for actinomycetes, promoting the survival and growth of actinomycetes. As the nutrients are absorbed and utilized, some substances that can neutralize acidic conditions are produced, further promoting the reproduction of actinomycetes, so that actinomycetes and lactobacilli reach an ecological balance and coexist in an environment rich in various small molecule nutrients, that is, the organic bio-fertilizer is obtained. Through experiments, it is found that the organic bio-fertilizer prepared by the present invention can significantly increase the potato yield and tuber starch content during potato planting, providing a basis for the application of potato starch in different fields. Detailed implementation manners

[0021] The various exemplary implementation manners of the present invention will be described in detail below. This detailed description should not be regarded as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and implementation schemes of the present invention.

[0022] It should be understood that the terms used in the present invention are only for describing specific implementation manners and are not used to limit the present invention. In addition, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0023] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0024] Without departing from the scope or spirit of the present invention, various improvements and changes can be made to the specific embodiments of the description of the present invention, which will be obvious to those skilled in the art. Other embodiments obtained from the description of the present invention will be obvious to those skilled in the art. The description and examples of the present invention are merely exemplary.

[0025] Regarding the use of "comprising", "including", "having", "containing", etc. in this article, they are all open-ended terms, that is, they are meant to include but not limited to.

[0026] The main strains involved in the following examples:

[0027] Actinomycetes (Microbacterium testaceum) SCAUT009, with the preservation number of GDMCC No: 61508, purchased from the Guangdong Institute of Microbiology. Lactobacillus plantarum HM-20, with the preservation number of CGMCC No.6744, purchased from the China General Microbiological Culture Collection Center. The public can purchase the above strains from the above preservation units.

[0028] Preparation of Actinomycetes SCAUT009 inoculant: After activating Actinomycetes SCAUT009 on Gao's No.1 solid medium, transfer it to Gao's No.1 liquid medium according to an inoculation amount of 8%, ferment and culture at 30 °C for 4 d, collect the bacterial liquid, and freeze-dry it to make bacterial powder for standby.

[0029] Preparation of Lactobacillus plantarum HM-20 inoculant: After activating Lactobacillus plantarum HM-20 on LB solid medium, transfer it to LB liquid medium according to an inoculation amount of 5% and culture for 3 d, collect the bacterial liquid, and freeze-dry it to make bacterial powder for standby.

[0030] The green seedlings and tail vegetables in the following examples are illustrated by taking Chinese cabbage green seedlings and tail vegetables as an example.

[0031] Example 1 A production method of organic biological fertilizer for green seedlings and tail vegetables

[0032] Mix the green seedlings and tail vegetables according to a mass ratio of 1:1, crush them into small pieces (0.5 cm * 0.5 cm), and then mix them with yellow mealworm feces and Lactobacillus plantarum HM-20, adjust the water content to 50%, and perform anaerobic fermentation at a constant temperature of 32 °C for 10 h to obtain fermentation product I; the mass ratio of the green seedlings and tail vegetables mixture, yellow mealworm feces, and Lactobacillus plantarum HM-20 is 3:1:0.5.

[0033] Add actinomycetes SCAUT009 to the above fermentation product I. Intermittently stir at a speed of 70 rpm for 20 minutes every 1 hour, and continue the constant-temperature fermentation culture at 30 °C for 24 hours after the temperature drops. The obtained fermentation product II is the organic bio-fertilizer for green seedlings and tail vegetables. Among them, the mass ratio of fermentation product I to actinomycetes SCAUT009 is 4:2.

[0034] Example 2 A production method of organic bio-fertilizer for green seedlings and tail vegetables

[0035] Mix green seedlings and tail vegetables according to a mass ratio of 1:1, crush them into small pieces (0.5 cm * 0.5 cm), and then mix them with yellow mealworm feces and Lactobacillus plantarum HM-20. Adjust the water content to 55%, and carry out constant-temperature anaerobic fermentation at 32 °C for 12 hours to obtain fermentation product I. The mass ratio of the green seedling and tail vegetable mixture, yellow mealworm feces, and Lactobacillus plantarum HM-20 is 4:1:0.5.

[0036] Add actinomycetes SCAUT009 to the above fermentation product I. Intermittently stir at a speed of 75 rpm for 20 minutes every 1 hour, and continue the constant-temperature fermentation culture at 30 °C for 30 hours after the temperature drops. The obtained fermentation product II is the organic bio-fertilizer for green seedlings and tail vegetables. Among them, the mass ratio of fermentation product I to actinomycetes SCAUT009 is 4.5:2.

[0037] Example 3 A production method of organic bio-fertilizer for green seedlings and tail vegetables

[0038] Mix green seedlings and tail vegetables according to a mass ratio of 1:1, crush them into small pieces (0.5 cm * 0.5 cm), and then mix them with yellow mealworm feces and Lactobacillus plantarum HM-20. Adjust the water content to 55%, and carry out constant-temperature anaerobic fermentation at 32 °C for 12 hours to obtain fermentation product I. The mass ratio of the green seedling and tail vegetable mixture, yellow mealworm feces, and Lactobacillus plantarum HM-20 is 5:2:0.5.

[0039] Add actinomycetes SCAUT009 to the above fermentation product I. Intermittently stir at a speed of 75 rpm for 20 minutes every 1 hour, and continue the constant-temperature fermentation culture at 30 °C for 36 hours after the temperature drops. The obtained fermentation product II is the organic bio-fertilizer for green seedlings and tail vegetables. Among them, the mass ratio of fermentation product I to actinomycetes SCAUT009 is 5:2.

[0040] Example 4 A production method of organic bio-fertilizer for green seedlings and tail vegetables

[0041] Mix the green seedlings and tail vegetables in a mass ratio of 1:1, crush them into small pieces (0.5 cm * 0.5 cm), and then mix them with yellow mealworm feces and Lactobacillus plantarum HM-20. Adjust the water content to 55%, and carry out anaerobic fermentation at a constant temperature of 32 °C for 12 h to obtain Fermentation Product I; the mass ratio of the green seedling and tail vegetable mixture, yellow mealworm feces, and Lactobacillus plantarum HM-20 is 3:1.5:0.5.

[0042] Add actinomycete SCAUT009 to the above Fermentation Product I. Intermittently stir at a speed of 75 rpm for 20 min every 1 h, and continue the constant-temperature fermentation culture at 30 °C for 40 h. The obtained Fermentation Product II is the organic bio-fertilizer of green seedlings and tail vegetables; among them, the mass ratio of Fermentation Product I and actinomycete SCAUT009 is 4.3:2.

[0043] Example 5 A production method of organic bio-fertilizer for green seedlings and tail vegetables

[0044] Mix the green seedlings and tail vegetables in a mass ratio of 1:1, crush them into small pieces (0.5 cm * 0.5 cm), and then mix them with yellow mealworm feces and Lactobacillus plantarum HM-20. Adjust the water content to 55%, and carry out anaerobic fermentation at a constant temperature of 32 °C for 12 h to obtain Fermentation Product I; the mass ratio of the green seedling and tail vegetable mixture, yellow mealworm feces, and Lactobacillus plantarum HM-20 is 4.8:1.6:0.5.

[0045] Add actinomycete SCAUT009 to the above Fermentation Product I. Intermittently stir at a speed of 75 rpm for 20 min every 1 h, and continue the constant-temperature fermentation culture at 30 °C for 30 h. The obtained Fermentation Product II is the organic bio-fertilizer of green seedlings and tail vegetables; among them, the mass ratio of Fermentation Product I and actinomycete SCAUT009 is 4.5:2.

[0046] Example 6 A production method of organic bio-fertilizer for green seedlings and tail vegetables

[0047] Mix the green seedlings and tail vegetables in a mass ratio of 1:1, crush them into small pieces (0.5 cm * 0.5 cm), and then mix them with yellow mealworm feces and Lactobacillus plantarum HM-20. Adjust the water content to 60%, and carry out anaerobic fermentation at a constant temperature of 32 °C for 15 h to obtain Fermentation Product I; the mass ratio of the green seedling and tail vegetable mixture, yellow mealworm feces, and Lactobacillus plantarum HM-20 is 5:2:0.5.

[0048] Add actinomycete SCAUT009 to the above Fermentation Product I. Intermittently stir at a speed of 80 rpm for 20 min every 1 h, and continue the constant-temperature fermentation culture at 30 °C for 48 h. The obtained Fermentation Product II is the organic bio-fertilizer of green seedlings and tail vegetables; among them, the mass ratio of Fermentation Product I and actinomycete SCAUT009 is 5:2.

[0049] Comparative Example 1

[0050] The difference from Example 2 is that when preparing Fermentation Product I, Tenebrio molitor feces is not used. Other operation steps are the same.

[0051] Comparative Example 2

[0052] The difference from Example 2 is that when preparing Fermentation Product I, Lactobacillus plantarum HM-20 is not used. Other operation steps are the same.

[0053] Comparative Example 3

[0054] The difference from Example 2 is that the addition timing of Lactobacillus plantarum is adjusted. The specific operation is as follows:

[0055] Mix green seedlings and tail vegetables in a mass ratio of 1:1, crush them into small pieces (0.5 cm * 0.5 cm), then mix them with Tenebrio molitor feces, adjust the water content to 55%, and perform anaerobic fermentation at a constant temperature of 32 °C for 12 h to obtain Fermentation Product I; the mass ratio of the green seedling and tail vegetable mixture to Tenebrio molitor feces is 4:1.

[0056] Add Actinomyces SCAUT009 and Lactobacillus plantarum HM-20 to the above Fermentation Product I, intermittently stir at a speed of 75 rpm for 20 min every 1 h, and continue the constant-temperature fermentation culture at a temperature reduced to 30 °C. The obtained Fermentation Product II is the organic bio-fertilizer of green seedlings and tail vegetables; among them, the mass ratio of Fermentation Product I to Actinomyces SCAUT009 is 4.5:2, and the mass ratio of the Lactobacillus plantarum HM-20 to the green seedling and tail vegetable mixture is 0.5:4.

[0057] Comparative Example 4

[0058] The difference from Example 2 is that single-strain Lactobacillus plantarum fermentation is used. The specific steps are as follows:

[0059] Mix green seedlings and tail vegetables in a mass ratio of 1:1, crush them into small pieces (0.5 cm * 0.5 cm), then mix them with Tenebrio molitor feces and Lactobacillus plantarum HM-20, adjust the water content to 55%, and perform anaerobic fermentation at a constant temperature of 32 °C for 12 h to obtain Fermentation Product I; the mass ratio of the green seedling and tail vegetable mixture, Tenebrio molitor feces and Lactobacillus plantarum HM-20 is 4:1:0.5.

[0060] Comparative Example 5

[0061] The difference from Example 2 is that single-strain Actinomyces fermentation is used. The specific operation is as follows:

[0062] Mix the green seedlings and tail vegetables in a mass ratio of 1:1, crush them into small pieces (0.5 cm * 0.5 cm), then mix them with the feces of Tenebrio molitor and Actinomyces SCAUT009, adjust the water content to 55%, and ferment and culture at 30 °C for 30 h. Intermittently stir at a speed of 75 rpm for 20 min every 1 h to obtain Fermentation Product I, which is the organic bio-fertilizer of green seedlings and tail vegetables; the mass ratio of the green seedling and tail vegetable mixture, the feces of Tenebrio molitor, and Actinomyces SCAUT009 is 4:1:0.5.

[0063] Comparative Example 6

[0064] The difference from Example 2 is: no fermentation with bacteria, and the specific operation is as follows:

[0065] Mix the green seedlings and tail vegetables in a mass ratio of 1:1, crush them into small pieces (0.5 cm * 0.5 cm), then mix them with the feces of Tenebrio molitor, adjust the water content to 55%, and carry out constant-temperature anaerobic fermentation at 32 °C for 12 h to obtain Fermentation Product I; the mass ratio of the green seedling and tail vegetable mixture and the feces of Tenebrio molitor is 4:1.

[0066] Comparative Example 7

[0067] The difference from Example 2 is: use the purchased Lactobacillus plantarum and Actinomyces powder to replace each powder respectively, and the other operation steps are the same.

[0068] Adopt the plate counting method to measure the viable bacteria count of the organic bio-fertilizer products of green seedlings and tail vegetables prepared in the above Examples 1-6 and Comparative Examples 1-7. The results are shown in Table 1.

[0069] Table 1 Results of viable bacteria count

[0070] Experimental group Number of viable bacteria (cfu / g bio-fertilizer) Example 1 <![CDATA[1.89×10 10 > Example 2 <![CDATA[2.35×10 10 > Example 3 <![CDATA[1.70×10 10 > Example 4 <![CDATA[1.72×10 10 > Example 5 <![CDATA[1.80×10 10 > Example 6 <![CDATA[1.63×10 10 > Comparative Example 1 <![CDATA[1.76×10 9 > Comparative Example 2 <![CDATA[5.68×10 9 > Comparative Example 3 <![CDATA[1.02×10 10 > Comparative Example 4 <![CDATA[3.20×10 9 > Comparative Example 5 <![CDATA[6.51×10 9 > Comparative Example 6 <![CDATA[1.23×10 3 > Comparative Example 7 <![CDATA[3.54×10 9 >

[0071] As can be seen from the above experimental data, the selection of the strains used in the production process, their dosage, the timing of strain addition, and the selection of the fermentation substrate are crucial for the content of viable bacteria in the organic bio-fertilizer. The selection of strains affects the coexistence state. Too much or too little addition of strains may affect the coexistence state. The timing of addition directly affects the exertion of its function, and thus affects the number of viable bacteria in the final coexistence state. Therefore, the types of strains, addition amounts, addition timing, etc. determined through optimization and improvement in the present invention are all key conditions affecting the quality of the finally produced organic bio-fertilizer. Based on years of experience in organic bio-fertilizers, the inventor infers that for green seedlings and tail vegetables, they contain a large amount of water, a large amount of dietary fiber, as well as various amino acids, vitamins, trace elements such as calcium and zinc. After fermentation by Lactobacillus plantarum, the dietary fiber and other components in the cells of green seedlings and tail vegetables can be released and converted into small molecules that are easy to absorb. Since the dietary fiber in green seedlings and tail vegetables accounts for the vast majority, although it can be decomposed, transformed and utilized by Lactobacillus plantarum, it is not sufficient to provide stable and suitable environmental conditions for the growth and reproduction of Lactobacillus plantarum; while the feces of Tenebrio molitor are extremely dry and can absorb part of the water in green seedlings and tail vegetables. More importantly, the feces of Tenebrio molitor contain 80.35% of crude organic matter, and mainly crude protein. Therefore, the addition of the feces of Tenebrio molitor can provide sufficient nutrients for the growth and reproduction of Lactobacillus plantarum. The ability of single Lactobacillus plantarum to degrade crude protein and crude fiber is limited. With the addition of actinomycetes, it can effectively degrade and transform crude fiber and crude protein. Intermittent stirring reaction can provide good fermentation conditions for actinomycetes and does not affect the survival of lactic acid bacteria at the same time. As the fermentation progresses, it can promote the ecological balance between actinomycetes and Lactobacillus plantarum, coexist in an environment rich in nutrients, and thus provide an organic bio-fertilizer rich in nutrients and containing a high content of composite probiotics.

[0072] Experimental Example 7

[0073] Experimental location: Qianhuai Village, Weichang Manchu and Mongolian Autonomous County, Chengde City.

[0074] Experimental time: July - November 2023.

[0075] Experimental crop: Potato "Zhongshu No. 2".

[0076] Experimental groups: The organic bio-fertilizers prepared in Examples 1 - 6 and Comparative Examples 1 - 7, with the organic bio-fertilizer not prepared using the above groups as the blank control.

[0077] Usage method: Apply the organic bio-fertilizers of each group during the seedling stage, tuber formation stage, and tuber swelling stage respectively (the organic bio-fertilizers of each group are respectively formulated into a dilution of the organic bio-fertilizer, and the total viable bacteria content in the dilution is 1.5×10 9Irrigate the roots with about [[ID=]]cfu / mL (the dosage at each stage is 1.5 L / mu, 2.0 L / mu, and 2.0 L / mu respectively). The irrigation position is 5 - 8 cm away from the root, and the depth is 8 - 10 cm. For the blank control, irrigate the roots with the same amount of water. Select one mu of potatoes planted and managed under the same conditions in each group as the experimental field.

[0078] Determination indicators:

[0079] 1. After harvesting the potatoes by group, count the yield of each group.

[0080] 2. Randomly select 10 kg of potatoes from each group as experimental samples and measure their starch content. The measurement method is as follows:

[0081] (1) Sample treatment: Wash and chop the potatoes in each group, add an appropriate amount of cold water and stir evenly, filter the mixture, and retain the liquid part;

[0082] (2) Starch precipitation: Let the liquid part obtained in the previous step stand for a period of time until the potato starch precipitates to the bottom;

[0083] (3) Starch separation: Gently pour out the upper liquid in the above mixture and retain the potato starch precipitated at the bottom;

[0084] (4) Starch drying: Place the potato starch obtained in the previous step at a low temperature, such as room temperature or in a heater, and let it dry gradually until it is completely dry.

[0085] (5) Weigh the starch: Use a balance instrument to weigh the mass of the dried potato starch.

[0086] The results are shown in Table 2.

[0087] Table 2 Measurement results of the yield and starch content of each group

[0088] Grouping Yield (kg / mu) Starch content (g / 100g potato) Example 1 2307 19.2 Example 2 2550 23.4 Example 3 1920 17.2 Example 4 2100 17.6 Example 5 2201 18.4 Example 6 1858 16.8 Comparative Example 1 950 14.2 Comparative Example 2 1295 14.7 Comparative Example 3 1359 15.2 Comparative Example 4 1002 14.4 Comparative Example 5 1307 15.0 Comparative Example 6 886 14.1 Comparative Example 7 1107 14.5 Blank control 851 14.1

[0089] As can be seen from the above data, the use of the organic bio-fertilizer prepared by the present invention can significantly increase the yield and starch content of potatoes. The inventor infers that the main reason may be that the organic bio-fertilizer prepared by the present invention contains probiotics and the nutrients contained in the survival of probiotics, which can ensure the viable count of probiotics in the organic bio-fertilizer. At the same time, due to the addition of Tenebrio molitor feces, after the organic bio-fertilizer is regularly applied to the soil, the probiotics in the soil can be increased, the bacterial environment in the soil can be balanced, and the beneficial bacteria can be promoted to become the dominant bacteria in the soil, promoting the transformation of the parts of nutrients provided by the soil or externally that are not conducive to absorption, enhancing the absorption and utilization of nutrients by the implanted roots. At the same time, the bacteria themselves produce some enzymes, nutrients, etc., promoting the smooth progress of the plant metabolic pathway and further improving the ability to absorb, utilize and transform nutrients. In addition, the structure of Tenebrio molitor feces similar to fine sand and its good water absorption capacity can also regulate the soil structure, maintain soil moisture, provide a better soil environment for the growth of potato tubers, and be beneficial to their growth. Generally speaking, the organic bio-fertilizer of the present invention is produced under specific preparation conditions, which can provide more superior environmental conditions and nutritional conditions for the growth of potatoes, thereby increasing the potato yield and tuber starch content.

[0090] The embodiments described above are only descriptions of the preferred embodiments of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A production method of organic biological fertilizer for green seedlings and tail vegetables, characterized in that, It includes the following steps: After crushing the green seedlings and tail vegetables, mix them with yellow mealworm feces and Lactobacillus plantarum, and conduct anaerobic fermentation to obtain fermentation product I; Add actinomycetes to the fermentation product I, and continue fermentation culture with intermittent stirring. The obtained fermentation product II is the organic bio-fertilizer of green seedlings and tail vegetables; The mass ratio of the green seedlings and tail vegetables, yellow mealworm feces, and Lactobacillus plantarum is (3 - 5):(1 - 2):0.5, and the green seedlings and the tail vegetables are mixed in a mass ratio of 1:1; The mass ratio of the fermentation product I to the actinomycetes is (4 - 5):2; The Lactobacillus plantarum is Lactobacillus plantarum HM-20, and the preservation number is CGMCC No.6744; the actinomycetes is actinomycetes SCAUT009, and the preservation number is GDMCC No: 61508.

2. The production method according to claim 1, characterized in that, The conditions for the anaerobic fermentation culture are: fermentation culture at 32°C for 10 - 15 h.

3. The production method according to claim 1, characterized in that, The intermittent stirring is to stir for 20 min under the condition of 70 - 80 rpm every 1 h.

4. The production method according to claim 1, characterized in that, The conditions for the continued fermentation culture are: fermentation culture at 30°C for 24 - 48 h.

5. An organic biological fertilizer for green seedlings and tail vegetables, characterized in that, It is prepared by the production method described in any one of claims 1 - 4.

6. Use of the organic bio-fertilizer of green seedlings and tail vegetables as described in claim 5 in increasing the yield of potatoes.

7. Use of the organic bio-fertilizer of green seedlings and tail vegetables as described in claim 5 in increasing the starch content of potatoes.