A deep-sea animal capturing and long-term culture test device

Abstract

A kind of deep-sea animal capture and long-term culture test device, including culture cabin and capture cabin independent of each other, capture cabin is connected to the top surface of culture cabin by thread, culture cabin is internally installed with culture cabin telescopic rod assembly, the upper end of which is fixed with culture cabin sealing cover, the bottom surface of culture cabin is fixedly installed with culture cabin lower hatch cover by fastener, the lower end of culture cabin telescopic rod assembly is fixed with culture cabin lower hatch cover, and the closure and opening of culture cabin body are realized by controlling the extension and contraction of culture cabin telescopic rod assembly;Capture cabin is internally installed with capture cabin telescopic rod assembly, the lower end of which is installed with capture cabin sealing cover, the top surface of capture cabin is installed with capture cabin hatch cover by fastener, the upper end of capture cabin telescopic rod assembly is fixed with capture cabin hatch cover, and the closure and opening of capture cabin body are realized by controlling the extension and contraction of capture cabin telescopic rod assembly, the capture, transfer and bait feeding of deep-sea animal can be conveniently realized, the overall working reliability is good, the safety factor is high, and the sealing performance is good.

Description

A device for capturing and long-term culture of deep-sea animals Technical Field

[0001] This invention relates to the field of deep-sea animal capture and culture technology, and in particular to an experimental device for deep-sea animal capture and long-term culture. Background Technology

[0002] The deep-sea animal capture and culture experimental device is used for sampling, transferring, and terrestrial culture of deep-sea animals. The device simulates the high-pressure environment of the deep sea and regularly feeds the animals to achieve long-term culture, providing an experimental basis for deep-sea animal research.

[0003] Currently, sampling and culture devices in existing technologies are mostly connected by bolts. When the device is in a high-pressure environment, this places high demands on the structural design of the connection and makes it difficult to seal. Existing culture devices use ball valves for feeding and sample transfer. High-pressure, large-diameter ball valves are costly, have low safety, and are prone to leakage. Summary of the Invention

[0004] In response to the shortcomings of the existing production technologies, the applicant provides a deep-sea animal capture and long-term culture experimental device, which can conveniently realize the capture, transfer and feeding of deep-sea animals, with good overall reliability, high safety factor and good sealing performance.

[0005] The technical solution adopted in this invention is as follows:

[0006] A deep-sea animal capture and long-term culture experimental device includes an independent culture chamber and a capture chamber. The capture chamber is threadedly connected to the top surface of the culture chamber. A culture chamber telescopic rod assembly is installed inside the culture chamber, with a culture chamber sealing cover fixed to the upper end of the telescopic rod assembly. A lower cover of the culture chamber is fixed to the bottom surface of the culture chamber using fasteners. The lower end of the telescopic rod assembly is fixed to the lower cover. The culture chamber is opened and closed by controlling the extension and retraction of the telescopic rod assembly. Similarly, a capture chamber telescopic rod assembly is installed inside the capture chamber, with a capture chamber sealing cover installed to the lower end of the telescopic rod assembly. A capture chamber cover is fixed to the top surface of the capture chamber using fasteners. The upper end of the telescopic rod assembly is fixed to the capture cover. The capture chamber is opened and closed by controlling the extension and retraction of the telescopic rod assembly.

[0007] Its further technical solution lies in:

[0008] The culture chamber adopts an internally hollow spherical structure.

[0009] The capture chamber adopts an internally hollow cylindrical structure.

[0010] When the pressure inside the telescopic rod assembly of the culture chamber is less than the pressure inside the chamber, the telescopic rod retracts and the culture chamber opens. When the pressure inside the telescopic rod assembly of the culture chamber is greater than the pressure inside the chamber, the telescopic rod extends and the culture chamber closes.

[0011] In the sample transfer mode, both the culture chamber and the capture chamber are placed vertically, with the culture chamber at the bottom and the capture chamber at the top, and the two are connected by threads. In the feeding mode and the mode in which the bait enters the culture chamber, the culture chamber and the capture chamber are separated, and the upper cover of the culture chamber is connected to the culture chamber body by threads and a sealing ring. In the sampling mode, the capture chamber and the culture chamber are separated, and the sample is taken through the capture chamber.

[0012] The lower cover of the culture chamber is an integral structure, and the lower cover of the culture chamber is inverted "T" shape. The outer ring of the lower cover of the culture chamber is sealed to the bottom opening of the culture chamber body by the lower cover sealing ring.

[0013] The outer ring of the culture chamber sealing cover is equipped with a culture chamber sealing cover sealing ring.

[0014] The capture chamber cover is an integral structure with a "T" shape. The outer ring of the capture chamber cover is sealed to the top opening of the capture chamber body by a capture chamber cover sealing ring.

[0015] The outer ring of the capture chamber sealing cover is equipped with a capture chamber sealing cover sealing ring.

[0016] The central axes of the culture chamber telescopic rod assembly and the capture chamber telescopic rod assembly are in the same plane.

[0017] The beneficial effects of this invention are as follows:

[0018] This invention features a compact and rational structure, and is easy to operate. The telescopic rod assembly is arranged inside the chamber, reducing the number of external mechanisms. The capture chamber is opened by controlling the retraction of the telescopic rod assembly, thereby trapping animals. The culture chamber is opened by controlling the retraction of the telescopic rod assembly, allowing animal samples or bait to enter. The extension of the telescopic rod assemblies of the capture and culture chambers can be controlled by using a sealing cover to close the capture and culture chambers.

[0019] The present invention features a vertically arranged capture chamber and culture chamber, allowing animal samples and feed to be transferred into the culture chamber under the influence of gravity during sample transfer and feeding operations.

[0020] This invention utilizes two sets of telescopic rod assemblies to achieve baiting and sample transfer, which is safer and cheaper than ball valves.

[0021] The deep-sea animal capture and culture experimental device of the present invention can modify the channel diameter according to the animal samples and bait to make it easier for them to pass through. Attached Figure Description

[0022] Figure 1 is a schematic diagram of the sample transfer working condition of the present invention.

[0023] Figure 2 is a schematic diagram of the internal structure of the sample transfer process of the present invention.

[0024] Figure 3 is a schematic diagram of the internal structure of the sample transfer condition (transfer process) of the present invention.

[0025] Figure 4 is a structural schematic diagram of the capture chamber (sealed state) of the present invention.

[0026] Figure 5 is a schematic diagram of the capture chamber (sampling condition) of the present invention.

[0027] Figure 6 is a schematic diagram of the structure of the culture chamber (pressure-holding culture condition) of the present invention.

[0028] Figure 7 is a schematic diagram of the structure of the culture chamber (feeding condition) of the present invention.

[0029] Figure 8 is a schematic diagram of the structure of the culture chamber of the present invention (the working condition of the feed entering the culture chamber).

[0030] Among them: 1. Culture chamber; 2. Capture chamber;

[0031] 11. Lower cover of the culture chamber; 12. Telescopic rod assembly of the culture chamber; 13. Culture chamber body; 14. Sealing cover of the culture chamber; 15. Upper cover of the culture chamber;

[0032] 111. Sealing ring for the lower cover of the culture chamber;

[0033] 141. Sealing ring for the incubation chamber sealing cover;

[0034] 21. Capture compartment hatch cover; 22. Capture compartment hull; 23. Capture compartment telescopic boom assembly; 24. Capture compartment sealing cover;

[0035] 211. Capture compartment hatch sealing ring;

[0036] 241. Seal ring on the capture chamber cover. Detailed Implementation

[0037] The specific embodiments of the present invention will now be described with reference to the accompanying drawings.

[0038] As shown in Figures 1-8, the deep-sea animal capture and long-term culture experimental device of this embodiment includes an independent culture chamber 1 and a capture chamber 2. The capture chamber 2 is threadedly connected to the top surface of the culture chamber 1. A culture chamber telescopic rod assembly 12 is installed inside the culture chamber 1. A culture chamber sealing cover 14 is fixed to the upper end of the culture chamber telescopic rod assembly 12. A culture chamber lower cover 11 is fixedly installed on the bottom surface of the culture chamber 1 by fasteners. The lower end of the culture chamber telescopic rod assembly 12 is fixed to the culture chamber lower cover 11. The culture chamber body 13 is closed and opened by controlling the extension and retraction of the culture chamber telescopic rod assembly 12. A capture chamber telescopic rod assembly 23 is installed inside the capture chamber 2. A capture chamber sealing cover 24 is installed at the lower end of the capture chamber telescopic rod assembly 23. A capture chamber cover 21 is installed on the top surface of the capture chamber 2 by fasteners. The upper end of the capture chamber telescopic rod assembly 23 is fixed to the capture chamber cover 21. The capture chamber body 22 is closed and opened by controlling the extension and retraction of the capture chamber telescopic rod assembly 23.

[0039] The culture chamber 1 adopts an internally hollow spherical structure.

[0040] The capture chamber 2 adopts an internally hollow cylindrical structure.

[0041] When the pressure inside the culture chamber telescopic rod assembly 12 is less than the pressure inside the chamber, the telescopic rod retracts and the culture chamber 1 opens. When the pressure inside the culture chamber telescopic rod assembly 12 is greater than the pressure inside the chamber, the telescopic rod extends and the culture chamber 1 closes.

[0042] In the sample transfer mode, both the culture chamber 1 and the capture chamber 2 are placed vertically, with the culture chamber 1 located at the bottom and the capture chamber 2 located at the top, and the two are connected by threads. In the feeding mode and the mode in which the bait enters the culture chamber 1, the culture chamber 1 and the capture chamber 2 are separated, and the upper cover 15 of the culture chamber is connected to the culture chamber body 13 by threads and a sealing ring. In the sampling mode, the capture chamber 2 is separated from the culture chamber 1, and the sample is taken through the capture chamber 2.

[0043] The lower cover 11 of the culture chamber is an integral structure. The lower cover 11 of the culture chamber is inverted "T" shape. The outer ring of the lower cover 11 of the culture chamber is sealed to the bottom opening of the culture chamber body 13 by the lower cover sealing ring 111.

[0044] The outer ring of the culture chamber sealing cover 14 is fitted with a culture chamber sealing cover sealing ring 141.

[0045] The capture compartment cover 21 is an integral structure with a "T" shape. The outer ring of the capture compartment cover 21 is sealed to the top opening of the capture compartment body 22 by the capture compartment cover sealing ring 211.

[0046] The outer ring of the capture chamber sealing cover 24 is fitted with a capture chamber sealing cover sealing ring 241.

[0047] The central axes of the culture chamber telescopic rod assembly 12 and the capture chamber telescopic rod assembly 23 are in the same plane.

[0048] The specific structure and function of the deep-sea animal capture and long-term culture experimental device described in this invention are as follows:

[0049] As shown in Figures 1-3, the sample transfer operation of the deep-sea animal capture and culture experimental device is shown. The culture chamber 1 and the capture chamber 2 are placed vertically, with the capture chamber 2 on top and the culture chamber 1 on the bottom, and are connected by threads.

[0050] The upper end of the culture chamber telescopic rod assembly 12 is connected to the culture chamber sealing cover 14, and the lower end is connected to the culture chamber lower cover 11. The telescopic movement is achieved by controlling the pressure inside the culture chamber telescopic rod assembly 12.

[0051] When the pressure inside the culture chamber telescopic rod assembly 12 is slightly less than the pressure inside the chamber, the telescopic rod retracts and the culture chamber 1 opens.

[0052] When the pressure inside the culture chamber telescopic rod assembly 12 is slightly greater than the pressure inside the chamber, the telescopic rod extends, and the culture chamber 1 is sealed.

[0053] The lower end of the capture chamber telescopic rod assembly 23 is connected to the capture chamber sealing cover 24, and the upper end is connected to the capture chamber cover 21. It is the same as the internal structure of the culture chamber 1. The telescopic rod is retracted by changing the pressure inside the assembly, thereby realizing the opening and closing of the capture chamber 2.

[0054] In actual work process:

[0055] (a) The actual working process of sample transfer is as follows:

[0056] Step 1: The culture chamber 1 is vertically fixed, and the capture chamber 2 is connected to it from above by threads. Both the culture chamber telescopic rod assembly 12 and the capture chamber telescopic rod assembly 23 are in the extended state, that is, both the culture chamber 1 and the capture chamber 2 are in the closed state, and the pressure inside the two chambers is the same as the deep-sea environment in which the animals are located.

[0057] Step 2: Pressurize the space between the culture chamber sealing cover 14 and the capture chamber sealing cover 24 to make the pressure in this part the same as that in the two chambers.

[0058] Step 3: Reduce the pressure in the culture chamber telescopic rod assembly 12 and the capture chamber telescopic rod assembly 23, so that the telescopic rods contract under the action of pressure difference, and the culture chamber 1 and the capture chamber 2 are in the open state.

[0059] Step 4: Under the influence of gravity, the animal sample enters the culture chamber 1 from top to bottom.

[0060] Figures 4 and 5 show the structural schematic diagrams of the sealed state and sampling conditions of the capture chamber 2 of the present invention.

[0061] (II) The actual working process of the sampling condition is as follows:

[0062] Step 1: The capture chamber telescopic rod assembly 23 is in the extended state, and the capture chamber 2 is in the closed state. The capture chamber 2 is pressurized to make its internal pressure the same as the deep-sea environment where the animal is located.

[0063] Step 2: When the capture chamber 2 is in the deep sea, reduce the pressure inside the capture chamber telescopic rod assembly 23, the telescopic rod retracts, the capture chamber 2 is in the open state, and the animal is lured into the chamber (the luring mechanism is not shown).

[0064] Step 3: Once a sufficient number of animals have entered the capture chamber 2, increase the pressure inside the capture chamber telescopic rod assembly 23. The telescopic rod extends, and the capture chamber 2 is sealed. The pressure inside the chamber is always the same as the deep-sea environment where the animals are located.

[0065] Figure 6 shows the pressure-maintaining culture condition of culture chamber 1. The upper end of the culture chamber sealing cover 14 is in contact with the air and is under normal pressure, while the lower end is under high pressure. Under the action of the pressure difference between the inside and outside, culture chamber 1 forms a closed space.

[0066] Figures 7 and 8 show the feeding and feeding conditions of the culture chamber 1 and the feeding conditions of the feed entering the culture chamber. The upper cover 15 of the culture chamber is connected to the body 13 of the culture chamber by threads.

[0067] (III) The actual working process of feeding and feeding into the culture chamber is as follows:

[0068] Step 1: The telescopic rod assembly 12 of the culture chamber extends, the culture chamber 1 is in a closed state, feed is put into the space above the sealing cover 14 of the culture chamber, and the upper cover 15 of the culture chamber is tightened.

[0069] Step 2: Pressurize the space between the sealing cover 14 of the culture chamber and the upper cover 15 of the culture chamber to the same pressure as inside the culture chamber 1.

[0070] Step 3: Reduce the pressure inside the telescopic rod assembly 12 of the culture chamber, the telescopic rod retracts, and the feed enters the culture chamber 1 under the action of gravity.

[0071] Step 4: After all the bait has entered the culture chamber 1, increase the pressure inside the culture chamber telescopic rod assembly 12 to make it extend and seal the culture chamber 1.

[0072] Step 5: Reduce the pressure between the culture chamber sealing cover 14 and the upper cover 15 of the culture chamber to normal pressure, and remove the upper cover 15 of the culture chamber.

[0073] The above description is an explanation of the present invention and not a limitation thereof. The scope of the present invention is defined by the claims. Within the scope of protection of the present invention, any form of modification may be made.

Claims

1. A method for using a deep-sea animal capture and long-term culture experimental device, characterized in that: The system includes an independent culture chamber (1) and a capture chamber (2). The capture chamber (2) is threadedly connected to the top surface of the culture chamber (1). A culture chamber telescopic rod assembly (12) is installed inside the culture chamber (1). A culture chamber sealing cover (14) is fixed to the upper end of the culture chamber telescopic rod assembly (12). A culture chamber lower cover (11) is fixed to the bottom surface of the culture chamber (1) by fasteners. The lower end of the culture chamber telescopic rod assembly (12) is fixed to the culture chamber lower cover (11). The culture chamber body (13) is realized by controlling the extension and retraction of the culture chamber telescopic rod assembly (12). The capture chamber (2) is closed and opened; a capture chamber telescopic rod assembly (23) is installed inside the capture chamber (2), a capture chamber sealing cover (24) is installed at the lower end of the capture chamber telescopic rod assembly (23), a capture chamber cover (21) is installed on the top surface of the capture chamber (2) by fasteners, and the upper end of the capture chamber telescopic rod assembly (23) is fixed to the capture chamber cover (21). The capture chamber body (22) is closed and opened by controlling the extension and retraction of the capture chamber telescopic rod assembly (23); when the pressure inside the culture chamber telescopic rod assembly (12) is less than the pressure inside the chamber, the telescopic rod retracts, and the culture chamber ( 1) When the pressure inside the telescopic rod assembly (12) of the culture chamber is greater than the pressure inside the chamber, the telescopic rod extends and the culture chamber (1) is closed. In the sample transfer condition, both the culture chamber (1) and the capture chamber (2) are placed vertically, with the culture chamber (1) located at the bottom and the capture chamber (2) located at the top, and the two are connected by threads. In the feeding condition and the condition where the bait enters the culture chamber (1), the culture chamber (1) and the capture chamber (2) are separated, and the upper cover (15) of the culture chamber and the body (13) of the culture chamber are connected by threads and a sealing ring. In the sampling condition, the capture chamber (2) and the culture chamber are connected by threads and a sealing ring. (1) Separation, sampling is carried out through the capture chamber (2); The actual working process of sample transfer is as follows: First step: The culture chamber (1) is fixed vertically, and the capture chamber (2) is connected to it from above by threads. The culture chamber telescopic rod assembly (12) and the capture chamber telescopic rod assembly (23) are both in the extended state, that is, the culture chamber (1) and the capture chamber (2) are both in the closed state, and the pressure inside the two chambers is the same as the deep-sea environment where the animal is located; Second step: Pressurize the space between the culture chamber sealing cover (14) and the capture chamber sealing cover (24) so ​​that the pressure in this part is the same as that inside the two chambers; Step 3: Reduce the pressure in the culture chamber telescopic rod assembly (12) and the capture chamber telescopic rod assembly (23), so that the telescopic rod contracts under the action of pressure difference, and the culture chamber (1) and the capture chamber (2) are in an open state; Step 4: Under the action of gravity, the animal enters the culture chamber (1) from top to bottom; The actual working process of the sampling condition is as follows: Step 1: The capture chamber telescopic rod assembly (23) is in an extended state, the capture chamber (2) is in a closed state, and the capture chamber (2) is pressurized so that its internal pressure is the same as the deep sea environment where the animal is located; Step 2: When the capture chamber (2) is located in the deep sea, reduce the pressure in the capture chamber telescopic rod assembly (23), the telescopic rod contracts, the capture chamber (2) is in an open state, and the animal is lured into the chamber; Step 3: When enough animals enter the capture chamber (2), increase the pressure inside the capture chamber telescopic rod assembly (23), the telescopic rod extends, the capture chamber (2) is closed, and the pressure inside the chamber is always the same as the deep-sea environment where the animals are located; The actual working process of the feeding condition and the feeding condition of the feed entering the culture chamber is as follows: Step 1: The culture chamber telescopic rod assembly (12) extends, the culture chamber (1) is closed, feed is put into the space above the culture chamber sealing cover (14), and the upper cover of the culture chamber (15) is tightened; Step 2: Pressurize the space between the culture chamber sealing cover (14) and the upper cover of the culture chamber (15) to the same pressure as inside the culture chamber (1); Step 3: Reduce the pressure inside the telescopic rod assembly (12) of the culture chamber, the telescopic rod retracts, and the bait enters the culture chamber (1) under the action of gravity; Step 4: After all the bait has entered the culture chamber (1), increase the pressure inside the telescopic rod assembly (12) of the culture chamber to make it extend and seal the culture chamber (1); Step 5: Reduce the pressure between the sealing cover (14) of the culture chamber and the upper cover (15) of the culture chamber to normal pressure, and remove the upper cover (15) of the culture chamber.

2. The method of using the deep-sea animal capture and long-term culture experimental device as described in claim 1, characterized in that: The culture chamber (1) adopts an internally hollow spherical structure.

3. The method of using the deep-sea animal capture and long-term culture experimental device as described in claim 1, characterized in that: The capture chamber (2) adopts an internally hollow cylindrical structure.

4. The method of using the deep-sea animal capture and long-term culture experimental device as described in claim 1, characterized in that: The lower cover (11) of the culture chamber is an integral structure. The lower cover (11) of the culture chamber is in the shape of an inverted "T". The outer ring of the lower cover (11) of the culture chamber is sealed to the bottom opening of the culture chamber body (13) by the lower cover sealing ring (111).

5. The method of using the deep-sea animal capture and long-term culture experimental device as described in claim 1, characterized in that: The outer ring of the culture chamber sealing cover (14) is equipped with a culture chamber sealing cover sealing ring (141).

6. The method of using the deep-sea animal capture and long-term culture experimental device as described in claim 1, characterized in that: The capture chamber cover (21) is an integral structure. The capture chamber cover (21) is in the shape of a "T". The outer ring of the capture chamber cover (21) and the top opening of the capture chamber body (22) are sealed by the capture chamber cover sealing ring (211).

7. The method of using the deep-sea animal capture and long-term culture experimental device as described in claim 1, characterized in that: The outer ring of the capture chamber sealing cover (24) is fitted with a capture chamber sealing cover sealing ring (241).

8. The method of using the deep-sea animal capture and long-term culture experimental device as described in claim 1, characterized in that: The central axes of the culture chamber telescopic rod assembly (12) and the capture chamber telescopic rod assembly (23) are in the same plane.

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