A Chinese medicine compound sponge and mask for relieving anxiety and preparation method thereof

Abstract

The present invention belongs to the technical field of traditional Chinese medicine, and discloses a sponge of a Chinese medicine compound composition for relieving anxiety, a mask and a preparation method thereof. The preparation method comprises the following steps: S1: preparing a filtrate of a Chinese medicine compound composition for relieving anxiety; S2: preparing a sponge layer carrier; S3: preparing a sponge layer of a Chinese medicine compound composition for relieving anxiety. The mask comprises a sponge layer of a Chinese medicine compound composition, and the filtrate of the freeze-dried Chinese medicine compound composition is uniformly filled in the porous structure of the sponge layer carrier, and the effective components of the filtrate Chinese medicine compound composition are slowly released with the airflow passing through the porous structure. The present invention prepares a medicated sponge and a mask from the filtrate of the Chinese medicine compound composition that can be slowly volatilized and released into the respiratory tract, which is convenient for daily use, does not affect normal life, conforms to people's living habits, does not require special medical treatment, and has a good effect on preventing and relieving anxiety; and can quantitatively screen out the optimal Chinese medicine compound composition.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine, and in particular to a Chinese medicine compound sponge and a mask for relieving anxiety and a preparation method thereof. Background Art

[0002] Anxiety disorder, also known as anxiety neurosis, is the most common type of neurosis. It is characterized by the experience of anxiety. It is an emotional disorder characterized by persistent or recurring anxiety. This anxiety is not based on real threats in real life, or is disproportionate to the severity of the threats faced. It can usually be divided into two forms: chronic anxiety, i.e. generalized anxiety, and acute anxiety, i.e. panic attack. The main manifestations are: nervous worry without a clear objective object, restlessness, and symptoms of autonomic dysfunction, such as palpitations, hand tremors, sweating, frequent urination, etc., and motor restlessness. Pay attention to distinguishing normal anxiety. If the severity of anxiety is obviously inconsistent with objective facts or situations, or lasts too long, it may be pathological anxiety. The cause of anxiety disorder is still unclear, and it may be related to genetic factors, personality characteristics, cognitive processes, adverse life events, biochemistry, and physical diseases.

[0003] In daily life, generalized anxiety (chronic anxiety) is the most common. In addition to the characteristics of neurosis, it must also have persistent generalized anxiety as the main clinical phase. Mild anxiety disorder meets the following two points, and severe anxiety meets the third point: (1) Frequent or persistent fear or anxiety without a clear object and fixed content; (2) accompanied by autonomic nervous system symptoms or motor restlessness. (3) Social function is impaired, and the patient feels pain because it is unbearable and cannot be relieved. The course of the disease meets the above symptoms for at least 6 months.

[0004] Anxiety disorders are harmful in many ways. They not only affect the patient's mental state, but also their physical health and quality of life: (1) Harm to mental health: Long-term and severe anxiety may damage the prefrontal cortex, affecting the ability to recognize and control emotions. In severe cases, it may lead to cognitive decline or even dementia. (2) Harm to physical health: Anxiety may cause a variety of physical symptoms, such as insomnia, headaches, back pain, etc., and may increase the risk of diseases such as cardiovascular disease and digestive system diseases. (3) Reduced quality of life: Anxiety may interfere with the patient's daily life and work, reducing work efficiency and life satisfaction. (4) Deterioration of interpersonal relationships: Anxiety may cause patients to be irritable and suspicious, affecting their relationships with family and colleagues.

[0005] Therefore, anxiety is a disease that needs to be taken seriously and treated. If anxiety persists and affects daily life, professional medical help must be sought in time to alleviate symptoms and improve the quality of life. However, in real life, due to the widespread existence of chronic anxiety and people's habitual attitude towards it, as well as the cumbersome and inconvenient medical treatment, people are usually reluctant to go to the hospital for chronic anxiety alone, which often delays the treatment of chronic anxiety and worsens the condition.

[0006] Although there are many drugs for treating and intervening in anxiety disorders in the prior art, they are often not special drugs that are easy to use, carry, and do not affect daily life, and are not in line with people's daily living habits. In addition, there are many Chinese herbal composition formulas for treating and intervening in anxiety disorders, but there is a lack of effective modern effect verification and screening methods, making it difficult to quantitatively determine the formula with the best universality and optimal effect. Summary of the invention

[0007] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a Chinese medicine compound composition sponge and mask for relieving anxiety and a preparation method thereof, which is convenient for daily use, does not affect normal life, conforms to people's living habits, and does not require special medical treatment; and can quantitatively screen out the optimal Chinese medicine compound composition to solve the problems existing in the above-mentioned prior art.

[0008] In order to achieve the above object, the technical solution adopted by the present invention is:

[0009] A method for preparing a sponge layer of a traditional Chinese medicine compound composition for relieving anxiety, characterized in that it comprises the following steps:

[0010] S1: Preparation of filtrate of traditional Chinese medicine compound composition for relieving anxiety

[0011] Preparing a Chinese medicine compound composition, including: Patchouli, Artemisia argyi, Mint, Acorus calamus, Atractylodes macrocephala, Ligusticum chuanxiong, and raw Pollen Typhae;

[0012] Decoction: add water 5 times the weight of the Chinese medicinal materials, heat and reflux three times, soak for 0.5 hours for the first time, heat and reflux for 1.5 hours, heat and reflux for 1 hour for the second and third times, filter and combine the three filtrates, and then reduce the pressure and concentrate the filtrate to a concentration of 1g / mL to obtain the filtrate of the Chinese medicine compound composition, and cool it for later use;

[0013] S2: Preparation of sponge layer carrier

[0014] Preparation of mixed solution: carboxylated chitosan and gelatin were weighed in a mass ratio of 1:1, dissolved in deionized water to prepare a mixed solution with a mass concentration of 2%, heated to 37°C in a water bath, swollen for 24 hours, and then 0.25wt% of genipin and 3wt% of glycerol (both based on the mass of the mixed system) were added and magnetically stirred for 30 minutes to obtain a gelatin mixed solution;

[0015] Low-temperature freezing: Pour the gelatin mixed solution into a layered molding mold with a wavy cross-section (single layer 170mm*90mm*2mm), put it into a low-temperature refrigerator (-40℃) for 24 hours, and then freeze it in a freeze dryer for 48 hours to obtain a sponge layer carrier;

[0016] S3: Preparation of sponge layer of Chinese medicine compound composition

[0017] The sponge layer carrier prepared in step S2 is immersed in the filtrate of the Chinese medicine compound composition, and is allowed to stand for a long time to allow the sponge to absorb the medicinal solution to saturation, and then freeze-dried again to obtain the sponge layer of the Chinese medicine compound composition for relieving anxiety.

[0018] The Chinese medicine compound composition prepared in step S1 specifically comprises: 5-10g of Patchouli, 5-10g of Artemisia argyi, 10-15g of Menthus fragrans, 10-15g of Acorus calamus, 15-20g of Atractylodes lancea, 10-15g of Ligusticum chuanxiong, and 10-15g of raw Pollen Typhae.

[0019] The step S1 also includes the steps of using an animal model to verify the effectiveness of the Chinese medicine compound composition and screening out the Chinese medicine compound composition with the best effect:

[0020] S1-1: Construct a model, divide mice into a control group, a model group, a positive drug group, and low-, medium-, and high-dose groups of different ratios of Chinese herbal compound compositions, feed them with the same feed, and test the behavior of mice in each group;

[0021] S1-2: The serum of mice was collected regularly, and the levels of 5-HT and CORT in the serum of mice were detected using an enzyme-linked immunosorbent assay kit;

[0022] S1-3: Compare the results obtained in S1-1 and S1-2, and screen out a group of Chinese herbal compound compositions with the best ratio of effects.

[0023] The optimal group of Chinese herbal compound compositions screened out in step S1-3 is: 10g of Patchouli, 10g of Artemisia argyi, 15g of Mentha, 15g of Acorus calamus, 20g of Atractylodes, 15g of Ligusticum chuanxiong, and 15g of raw Pollen Typhae.

[0024] A sponge layer of a Chinese herbal compound composition for relieving anxiety prepared by the aforementioned method has a thickness of 1 to 3 mm. The filtrate of the freeze-dried Chinese herbal compound composition is uniformly filled in the porous structure of the sponge layer carrier, and the effective ingredients of the filtrate Chinese herbal compound composition are slowly released along with the air flow passing through the porous structure.

[0025] A Chinese medicine compound composition mask for relieving anxiety, comprising a mask sheet and ear loops, characterized in that the mask sheet is a multi-layer structure, and comprises, from the outside to the inside: an outer non-woven fabric layer, a graphene sponge layer, the Chinese medicine compound composition sponge layer, and an inner non-woven fabric layer; wherein the graphene sponge layer and the Chinese medicine compound composition sponge layer both have wavy cross-sections, and the two sponge layers are parallel to each other, spaced apart, and have gaps between them.

[0026] The graphene sponge layer is prepared by the following method:

[0027] A1: Preparation of mixed solution: carboxylated chitosan and gelatin were weighed in a mass ratio of 1:1, dissolved in deionized water to prepare a mixed solution with a mass concentration of 2%, heated to 37°C in a water bath, swollen for 24 hours, then 0.25% genipin and 3% glycerol (based on the mass of the mixed system) were added, and magnetic stirring was performed for 30 minutes to obtain a gelatin mixed solution;

[0028] A2: Low temperature freezing: Pour the gelatin mixed solution into a layered molding mold (single layer 170mm*90mm*2mm), put it into a low temperature refrigerator (-40℃) for 24 hours, and then freeze it in a freeze dryer for 48 hours to obtain a sponge layer carrier;

[0029] A3: Immerse the sponge layer carrier obtained in step A2 into a graphene oxide solution with a concentration of 10 μg / mL, let it stand for a long time to allow the sponge to absorb the solution to saturation, and then freeze-dry it again to obtain a graphene sponge layer that relieves anxiety.

[0030] In the Chinese medicine compound sponge layer and the graphene sponge layer, the porosity of the sponge is 75%-85%.

[0031] The beneficial effects of the present invention are:

[0032] 1. The anxiety-relieving Chinese herbal compound sponge, mask and preparation method thereof provided by the present invention can prepare the filtrate of the Chinese herbal compound composition that can slowly evaporate and release into the respiratory tract into a medicinal sponge and mask, which is convenient for daily use, does not affect normal life, conforms to people's living habits, does not require special medical treatment, has a good effect on preventing and relieving anxiety; and can quantitatively screen out the optimal Chinese herbal compound composition.

[0033] 2. The anxiety-relieving Chinese herbal compound sponge, mask and preparation method thereof provided by the present invention prepare masks of different groups with filtrates of the Chinese herbal compound composition in different proportions, and then test their efficacy by combining animal modeling and other methods, so as to quantitatively screen out the optimal Chinese herbal compound composition.

[0034] 3. The anxiety-relieving Chinese herbal compound sponge and mask provided by the present invention and the preparation method thereof, wherein the filtrate of the Chinese herbal compound is prepared into a mask, and the results of the Self-Rating Anxiety Scale (SAS) twice before and after wearing the mask show that it can significantly improve people's anxiety. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a schematic diagram of the main structure of the Chinese medicine compound composition mask according to an embodiment of the present invention;

[0036] Figure 2 A schematic diagram of a partial structure of a mask made of a Chinese medicine compound composition according to an embodiment of the present invention;

[0037] Figure 3 This is a schematic diagram of the cross-sectional structure of a mask of a Chinese medicine compound composition in an embodiment of the invention;

[0038] Figure 4 This is a schematic diagram comparing the total distance traveled by each dosage group in an open field experiment of an animal model of an embodiment of the present invention;

[0039] Figure 5 This is a comparative schematic diagram of the central area residence time of each dosage group in the open field test of the animal model of the embodiment of the present invention;

[0040] Figure 6 This is a comparative schematic diagram of the percentage of times each dosage group entered the open arm in the elevated plus maze test of the animal model of the embodiment of the present invention;

[0041] Figure 7 This is a comparative schematic diagram of the percentage of time spent in the open arms of each dosage group in the elevated plus maze test of the animal model of the embodiment of the present invention;

[0042] Figure 8 This is a comparative diagram of 5-HT levels in serum of each dosage group in the animal model of the present invention;

[0043] Fig. 9 This is a schematic diagram comparing the 5-HT levels in the hippocampus of each dosage group in the animal model of the present invention;

[0044] Fig.10 Schematic diagram of comparison of CORT levels in serum of various dosage groups in the animal model of the present invention example.

[0045] exist Figure 4-10 In the figure, * is VS blank group, # is VS model group, 1 is blank group, 2 is model group, 3 is positive drug group, 4 is low-dose group of Chinese medicine compound, 5 is medium-dose group of Chinese medicine compound, and 6 is high-dose group of Chinese medicine compound.

[0046] Figure numerals: 1. mask piece; 2. nose bridge strip; 3. outer non-woven fabric layer; 4. graphene sponge layer; 5. Chinese medicine compound sponge layer; 6. inner non-woven fabric layer; 7. ear hook. DETAILED DESCRIPTION

[0047] The present invention is described in detail below in conjunction with the accompanying drawings and embodiments.

[0048] Example 1

[0049] The method for preparing the sponge layer of the anxiety-relieving traditional Chinese medicine compound composition provided in an embodiment of the present invention comprises the following steps:

[0050] S1: Preparation of filtrate of traditional Chinese medicine compound composition for relieving anxiety

[0051] First, within the following proportion range, 5-10g of Patchouli, 5-10g of Artemisia argyi, 10-15g of Mentha arvense, 10-15g of Acorus calamus, 15-20g of Atractylodes lancea, 10-15g of Ligusticum chuanxiong, and 10-15g of raw Pollen Typhae are prepared according to different proportions of Patchouli, Artemisia argyi, Mentha arvense, Acorus calamus, Atractylodes lancea, Ligusticum chuanxiong, and raw Pollen Typhae, and the Chinese medicine compound compositions are numbered respectively;

[0052] Among them, the formula of the Chinese medicine compound composition No. 1 is: Patchouli 5g, Artemisia argyi 10g, Mentha 12g, Acorus calamus 15g, Atractylodes 20g, Ligusticum chuanxiong 12.5g, and Raw Pollen Typhae 10g;

[0053] The formula of the Chinese medicine compound composition No. 2 is: Patchouli 8g, Artemisia argyi 5g, Mentha 10g, Acorus calamus 10g, Atractylodes 15g, Ligusticum chuanxiong 15g, and Pollen Typhae 12.5g;

[0054] The formula of the Chinese medicine compound composition No. 3 is: Patchouli 10g, Artemisia argyi 8g, Mentha 15g, Acorus calamus 12.5g, Atractylodes lancea 17.5g, Ligusticum chuanxiong 10g, and Pollen Typhae 15g;

[0055] The formula of the Chinese medicine compound composition No. 4 is: Patchouli 10g, Artemisia argyi 10g, Mentha 15g, Acorus calamus 15g, Atractylodes 20g, Ligusticum chuanxiong 15g, and Pollen Typhae 15g;

[0056] Then, the Chinese medicine compound compositions No. 1-4 were decocted in water respectively: 5 times the weight of the Chinese medicinal materials in each composition was added with water, heated and refluxed for three times, the first soaking was 0.5 hours and then heated and refluxed for 1.5 hours, the second and third heating and refluxed for 1 hour, the three filtrates were filtered and combined, and the filtrates were then concentrated under reduced pressure to a concentration of 1 g / mL to obtain the filtrates of the Chinese medicine compound compositions, which were numbered as filtrates No. 1-4 respectively, and cooled for standby use;

[0057] S2: Preparation of sponge layer carrier

[0058] Preparation of mixed solution: carboxylated chitosan and gelatin were weighed in a mass ratio of 1:1, dissolved in deionized water to prepare a solution with a mass concentration of 2% of the mixed solution, heated to 37°C in a water bath, swollen for 24 hours, and then 0.25% of genipin and 3% of glycerol (both based on the mass of the mixed system) were added and magnetically stirred for 30 minutes to obtain a gelatin mixed solution;

[0059] Low-temperature freezing: Pour the gelatin mixed solution into a layered molding mold with a wavy cross-section (single layer 170mm*90mm*2mm), put it into a low-temperature refrigerator (-40℃) for 24 hours, and then freeze it in a freeze dryer for 48 hours to obtain a sponge layer carrier;

[0060] S3: Preparation of sponge layer of Chinese medicine compound composition

[0061] The sponge layer carrier prepared in step S2 is immersed in the filtrate No. 1-4 of the Chinese medicine compound composition respectively, and is allowed to stand for a long time to allow the sponge to absorb the liquid medicine to saturation, and then freeze-dried again to prepare the Chinese medicine compound composition sponge layers for relieving anxiety, which are numbered as Chinese medicine compound composition sponge layers No. 1-4 respectively.

[0062] The step S1 also includes the steps of using an animal model to verify the effectiveness of the Chinese medicine compound composition and screening out the Chinese medicine compound composition with the best effect:

[0063] S1-1: Construct a model, divide mice into a control group, a model group, a positive drug group, and low-, medium-, and high-dose groups of different ratios of Chinese herbal compound compositions, feed them with the same feed, and test the behavior of mice in each group;

[0064] S1-2: The serum of mice was collected regularly, and the levels of 5-HT and CORT in the serum and hippocampus of mice were detected using an enzyme-linked immunosorbent assay kit;

[0065] S1-3: Compare the results obtained in S1-1 and S1-2, and screen out a group of Chinese herbal compound compositions with the best ratio of effects.

[0066] The optimal group of Chinese herbal compound compositions screened out in step S1-3 is: 10g of Patchouli, 10g of Artemisia argyi, 15g of Mentha, 15g of Acorus calamus, 20g of Atractylodes, 15g of Ligusticum chuanxiong, and 15g of raw Pollen Typhae.

[0067] A sponge layer of a Chinese herbal compound composition for relieving anxiety prepared by the aforementioned method, wherein the single layer thickness is 1 to 3 mm and the cross section is wavy. The 1-4 filtrates of the freeze-dried Chinese herbal compound composition are uniformly filled in the porous structure of the sponge layer carrier, and the effective ingredients of the filtrate Chinese herbal compound composition are slowly released along with the airflow passing through the porous structure.

[0068] A Chinese medicine compound composition mask for relieving anxiety, comprising a mask sheet 1 and ear loops 7, wherein the mask sheet 1 is a sandwich structure in which multiple layers of materials are stacked and then sewn, and comprises, from the outside to the inside, an outer non-woven fabric layer 3, a graphene sponge layer 4, a Chinese medicine compound composition sponge layer 5, and an inner non-woven fabric layer 6; wherein the graphene sponge layer 4 and the Chinese medicine compound composition sponge layer 5 both have wavy cross-sections, and the two sponge layers are parallel to each other and arranged at intervals, and a gap for ventilation is left between the outer surfaces of one side corresponding to each other; the outer surfaces of the graphene sponge layer 4 and the Chinese medicine compound composition sponge layer 5 are both rough surfaces with three-dimensional concave-convex structures, the cross-section of the concave-convex structures is arc-shaped, and the height of the concave-convex structures is 0.2-0.5 mm.

[0069] The graphene sponge layer is prepared by the following method:

[0070] A1: Preparation of mixed solution: carboxylated chitosan and gelatin were weighed in a mass ratio of 1:1, dissolved in deionized water to prepare a mixed solution with a mass concentration of 2%, heated to 37°C in a water bath, swollen for 24 hours, then 0.25% genipin and 3% glycerol (based on the mass of the mixed system) were added, and magnetic stirring was performed for 30 minutes to obtain a gelatin mixed solution;

[0071] A2: Low temperature freezing: Pour the gelatin mixed solution into a layered molding mold (single layer 170mm*90mm*2mm), put it into a low temperature refrigerator (-40℃) for 24 hours, and then freeze it in a freeze dryer for 48 hours to obtain a sponge layer carrier;

[0072] A3: Immerse the sponge layer carrier obtained in step A2 into a graphene oxide solution with a concentration of 10 μg / mL, let it stand for a long time to allow the sponge to absorb the solution to saturation, and then freeze-dry it again to obtain a graphene sponge layer that relieves anxiety.

[0073] In the Chinese medicine compound sponge layer and the graphene sponge layer, the porosity of the sponge is 75%-85%.

[0074] Example 2

[0075] See also Figures 1 to 10 The anxiety-relieving Chinese medicine composite sponge, mask and preparation method thereof provided in this embodiment are further described in more detail on the basis of Example 1. The difference between this embodiment and Example 1 is that:

[0076] 1. First, according to the proportions of Example 1, prepare the Chinese medicine compound composition sponge layers and the Chinese medicine compound composition masks numbered 1-4 respectively;

[0077] The components in the filtrate dried product of the Chinese medicine compound composition have the following functions in the compatibility:

[0078] Patchouli has the functions of aromatic dehumidification, harmonizing the stomach and stopping vomiting, relieving summer heat, and expelling filth. When combined with other components, it can ward off evil spirits and resolve epidemic qi; Atractylodes lancea dries dampness and dispels cold, and is often used for epidemic prevention;

[0079] Mugwort leaves are pungent, bitter, and warm in nature; aromatic, warm the meridians and stop bleeding, dispel cold and relieve pain, and remove dampness;

[0080] Both Artemisia argyi and Chuanxiong are pungent and warm, and can dispel dampness and cold;

[0081] Acorus calamus has a fragrant aroma that enters the spleen, awakens the spleen and removes turbidity;

[0082] Raw Puhuang promotes blood circulation and removes blood stasis;

[0083] Mint dispels wind-heat, clears the nasal passages, soothes the liver and relieves depression.

[0084] The above formulas all have aromatic volatile components. After being combined with each other, a small part of the volatile components directly enters the brain through the sense of smell, and most of them enter the human body through the respiratory tract and are absorbed and enter the human blood. They work together to quickly and lastingly relieve anxiety and depression.

[0085] Therefore, the Chinese medicine compound composition sponge layer and the Chinese medicine compound composition mask provided by the embodiments of the present invention, wherein the dried filtrate adsorbed in the Chinese medicine compound composition sponge layer, is based on the Chinese medicine epidemic prevention formula according to the compatibility rules of the Chinese medicine compound monarch, minister, assistant and envoy and the Chinese medicine absorption principle, and is combined with the brain clearing formula for formula optimization, and is further verified through animal experiments and questionnaires to obtain a Chinese medicine epidemic prevention formula that can prevent pathogens, relieve stress and anxiety, and refresh the mask.

[0086] 2. Animal experiments

[0087] The statistical processing method of the experimental data was as follows: GraphPad Prism 8 was used for statistical analysis and drawing. All statistical data were expressed as mean ± standard deviation (`x ± s), and one-way analysis of variance was used for comparison among multiple groups, and t-test was used for pairwise comparison. P < 0.05 was considered statistically significant.

[0088] 2.1 Model preparation and drug administration

[0089] The mice were divided into a control group, a model group, a positive drug group and a low, medium and high dose group of the Chinese medicine formula aromatherapy bag made of the sponge layer of the Chinese medicine compound composition, and were fed with the same feed respectively; the behavior of mice in each group was tested.

[0090] This experiment used chronic restraint stress to construct an anxiety model. Specific modeling method: a ventilation hole was drilled at the bottom of a 50 mL centrifuge tube to make a restraint device. When punching the hole, the hole arm should be as smooth as possible to avoid unnecessary harm to the mice and increase experimental uncertainty. At a fixed time every day, the mice were placed in the processed centrifuge tube to receive 6 hours of restraint stress. After the mice were placed in the centrifuge tube, the tube cap was tightened, and the centrifuge tube was placed horizontally and fixed to keep the pores unobstructed. This modeling lasted for 21 days to establish an anxiety model. The head was slightly raised to allow urine to be discharged smoothly.

[0091] The positive drug group was given 2.0 mg kg per day -1 ·d -1 Diazepam suspension was gavaged, and the blank control group was gavaged with an equal volume of normal saline. The Chinese medicine formula aromatherapy bag components are low, medium and high doses, and the formulas are No. 1-4 respectively. The following animal experiment is carried out using the preparation of the No. 4 Chinese medicine compound composition as an example. The specific formula of the No. 4 Chinese medicine compound composition is: 10g of Patchouli, 10g of Artemisia argyi, 15g of Mint, 15g of Acorus calamus, 20g of Atractylodes, 15g of Chuanxiong, and 15g of raw Pollen Typhae.

[0092] 2.2 Detection indicators

[0093] 2.2.1 Behavioral testing

[0094] 2.2.1.1 Open field experiment

[0095] On the 21st day of the experiment, an open field experiment was conducted. The experimental device consisted of an open field reaction box and an automatic data acquisition and processing system. The bottom area of ​​the open field reaction box was 1m 2 , divided into 4 areas, each of which includes a peripheral area and a central area. In a quiet environment, mice were placed in the center of the bottom surface of the four areas, and the camera above the reaction box was used to record and time the time the mice stayed in the central area within 5 minutes. The ANY-maze animal behavior analysis system was used to record animal activities throughout the process. After the experiment, the animals were taken out and the test area was sprayed with 10% ethanol to remove the remaining excrement and odor of the animals.

[0096] The effect of Chinese herbal medicine combination on open field test indicators is shown in the attached Figure 4-5 As shown in Table 1, the results of the open field test showed that after the modeling group formed anxiety, the time it took to enter the central area was the shortest and the distance it moved was the shortest; after modeling, the three dose groups treated with the No. 4 Chinese herbal compound composition had correspondingly increased residence time in the central area and total distance traveled, which was second only to the positive drug.

[0097] Table 1

[0098]

[0099] 2.2.1.2 Elevated plus maze

[0100] The elevated plus maze test was performed immediately after the open field test. Before the experiment, the mice were placed in the open field for 5 minutes, and then placed in the center of the maze with their heads facing one of the open arms. The activity of the mice in the elevated plus maze was tracked and recorded by a computer for 5 minutes. The percentage of open arm entries (OB%), that is, the value of open arm entries / (open arm entries + closed arm entries) X 100%, was used as an indicator to evaluate the anxiolytic effect of the drug.

[0101] The effect of Chinese herbal medicine combination on the parameters of elevated plus maze test is shown in the attached Figure 6-7 As shown in Table 2, the results of the elevated plus maze experiment showed that after modeling, the percentage of anxious mice entering the open arms and the percentage of time they stayed in the open arms were smaller than those of the blank group. After intervention with positive drugs and Chinese medicine components, the percentage of times they entered the open arms and the percentage of time they stayed in the open arms were significantly increased.

[0102] Table 2

[0103]

[0104] 2.2.1.3 Sugar water preference experiment

[0105] The animals are provided with two drinking solutions. One is sweetened with sucrose (or other sweeteners), while the other is plain drinking water. The animals' preference for the two solutions is analyzed based on a comprehensive measure of total fluid intake, frequency of intake, and time period of intake.

[0106] The effect of the Chinese medicine composition on the test index of sugar water preference is shown in Table 3. In the experiment, two drinking water devices were provided, one containing sucrose solution and the other containing ordinary water, and mice could choose freely. Under normal circumstances, mice tend to choose sweet sucrose solution. However, in the anxiety model caused by chronic stress, the preference of mice for sucrose solution will decrease. By detecting the preference of mice for sucrose solution, it can be used as an effective method to evaluate the symptoms of anhedonia and anxiety of animals.

[0107] Table 3

[0108]

[0109] 3.ELISA detection

[0110] After the behavioral experiment, blood was collected by removing the eyeballs, centrifuged at 4°C, 5000 rpm / min for 5 min, and the supernatant was obtained to obtain the test solution.

[0111] 3.1 Detection of 5-HT content

[0112] The 5-HT content in the above test solution was determined according to the instructions of the ELISA kit. The results of the effect of the Chinese medicine composition on the 5-HT level are shown in the attached Figure 8-9 .

[0113] 3.2 Detection of CORT content

[0114] The CORT content in the above test solution was determined according to the ELISA kit instructions. The results of the effect of the Chinese medicine composition on the CORT level are shown in the attached Fig.10 .

[0115] The 5-HT and cort in the serum of experimental mice, as well as the 5-HT in the hippocampus of the brain were measured by ELISA experiments. The results showed that the 5-HT and cort values ​​of the model group increased after suffering from anxiety, which was improved by medication and aromatherapy. The experiment proved that the Chinese herbal compound can improve the anxiety of mice. See Table 4 for details.

[0116] Table 4

[0117]

[0118] 4. Volunteers try questionnaire survey method

[0119] The compound was prepared into masks and randomly distributed to adult volunteers who felt anxious or had been diagnosed with anxiety in public places such as schools and hospitals for trial and testing. The masks were worn for 21 consecutive days, each time for 6 hours, and replaced with new masks every 7 days. The Self-Rating Anxiety Scale (SAS) was distributed and collected before and after wearing the masks. The data of the Self-Rating Anxiety Scale (SAS) were collected twice, and the results were compared. The preparation of the compound into masks can also improve people's anxiety. The anxiety situation before using the mask is shown in Table 5, and the anxiety situation after using the mask is shown in Table 6.

[0120] Table 5

[0121]

[0122] Table 6

[0123]

[0124] The anxiety-relieving Chinese medicine composite sponge and mask provided in the above embodiments of the present invention have the following indications: (1) patients who repeatedly visit relevant departments of a general hospital for headache, neck, back, waist and limb pain, and whose clinical examination and laboratory test results do not indicate organic lesions; (2) chronic pain caused by mental factors such as anxiety, phobia, hypochondriasis, depression, etc.; (3) chronic systemic pain caused by various reasons; (4) tension headache; (5) migraine; and contraindications include: (1) patients with severe arrhythmia or heart failure during or after myocardial infarction; (2) patients with severe diseases of major organs, such as liver and kidney dysfunction, respiratory failure, cerebral hemorrhage, cerebral infarction, and unstable diabetes; (3) patients in the onset of schizophrenia; (4) patients with severe intellectual disabilities and who do not cooperate with the examination.

[0125] Attachment: Self-Rating Anxiety Scale (SAS)

[0126]

[0127] Scoring and Interpretation:

[0128] 1. The assessment is based on a scoring system of 1 to 4;

[0129] 2. Add the scores of the 20 questions to get the total score, multiply the total score by 1.25, and round it to the nearest integer to get the standard score;

[0130] 3. The cut-off value for anxiety assessment is 50 points, 50-59 points are mild anxiety, 60-69 points are moderate anxiety, and 70 points and above are severe anxiety; the higher the score, the more obvious the anxiety tendency.

[0131] 1. Applicable objects

[0132] The SAS is suitable for adults with anxiety symptoms. Like the SDS, it has a wider applicability.

[0133] 2. Assessment methods and matters needing attention

[0134] Before the self-assessor makes the assessment, he should understand the entire scale filling method and the meaning of each question, and then make an independent self-assessment without being influenced by anyone.

[0135] Before the assessment begins, the staff will point to the SAS scale and tell him: There are 20 items below. Please read each item carefully to understand its meaning, and then tick the appropriate box according to your actual situation in the past week. There are 4 boxes after each item, representing no or very rarely (occurring), a small part of the time, quite a lot of the time, most of the time or all of the time.

[0136] If the assessor has a low level of education and cannot understand or read the SAS questions, the staff can read them to him one by one, and let the assessor make his own assessment independently. Generally, one assessment can be completed within ten minutes.

[0137] It should be noted that:

[0138] 1. The time frame for assessment should be emphasized as “now or in the past week”.

[0139] 2. At the end of the assessment, the staff should carefully check the self-assessment results and remind the self-assessors not to miss any item or tick the same item twice (i.e. do not repeat the assessment).

[0140] 3. SAS should be assessed once by the self-assessor before the start of treatment, and then at least once after treatment (or at the end of the study) so that the change in the self-assessor's symptoms can be analyzed through the change in the total SAS score. If the assessment is conducted during the treatment or study, the interval can be arranged by the researcher.

[0141] 3. Results Analysis

[0142] The main unified indicator of SAS is the total score. After the self-assessor has finished the assessment, the scores of each of the 20 items are added together to get the raw score. The raw score is converted by the following formula: y=int(1.25x); that is, the raw score is multiplied by 1.25 and the integer part is taken to get the standard score (index score, Y). Alternatively, the same conversion can be performed by looking up a table.

[0143] It must be emphasized that among the 20 items in SAS, the scoring of 5 items, namely 5, 9, 13, 17 and 19, must be calculated in reverse order.

[0144] It should be noted that the above embodiments describe only a part of the embodiments of the present invention, rather than all the embodiments. In other embodiments, within the scope of the present invention, the technical solutions obtained by selecting other components, proportions and process parameters can also achieve the technical effects recorded in the present invention, so they are not listed one by one.

[0145] The specific embodiments described above are only an introduction to the optimization method of the present invention, and are not intended to limit the scope of the present invention. It should be noted that, without departing from the spirit of the present invention, those skilled in the art may make appropriate adjustments or modifications to the technical solution of the present invention, which shall fall within the scope of protection determined by the claims of the present invention.

Claims

1. A method for preparing a sponge layer of a Chinese medicine compound composition for relieving anxiety, characterized in that: It includes the following steps: S1: Preparation of filtrate of traditional Chinese medicine compound composition for relieving anxiety Prepare a Chinese medicine compound composition, including: Patchouli, Artemisia argyi, Mint, Acorus calamus, Atractylodes macrocephala, Ligusticum chuanxiong, and raw Pollen Typhae; Decoction: add sufficient amount of water, soak and heat under reflux, combine three filtrates, and then reduce the pressure and concentrate the filtrates to obtain the filtrate of the Chinese medicine compound composition, and cool it for use; S2: Preparation of sponge layer carrier Preparation of mixed solution: weigh carboxylated chitosan and gelatin, dissolve in deionized water to prepare a mixed solution, heat in a water bath, swell, add genipin and glycerol, and stir magnetically to obtain a gelatin mixed solution; Low-temperature freezing: pouring the gelatin mixed solution into a layered molding mold with a wavy cross-section, freezing it in a low-temperature refrigerator, and then freeze-drying it in a freeze dryer to obtain a sponge layer carrier; S3: Preparation of sponge layer of Chinese medicine compound composition The sponge layer carrier prepared in step S2 is immersed in the filtrate of the Chinese medicine compound composition, and is allowed to stand for a long time to allow the sponge to absorb the medicinal solution to saturation, and then freeze-dried again to obtain a sponge layer of the Chinese medicine compound composition for relieving anxiety; The Chinese medicine compound composition prepared in step S1 specifically comprises: 5-10g of Patchouli, 5-10g of Artemisia argyi, 10-15g of Menthus fragrans, 10-15g of Acorus calamus, 15-20g of Atractylodes lancea, 10-15g of Ligusticum chuanxiong, and 10-15g of Pollen Typhae; The step S1 also includes the steps of using an animal model to verify the effectiveness of the Chinese medicine compound composition and screening out the Chinese medicine compound composition with the best effect: S1-1: Construct a model, divide mice into a control group, a model group, a positive drug group, and low-, medium-, and high-dose groups of different ratios of Chinese herbal compound compositions, feed them with the same feed, and test the behavior of mice in each group; S1-2: The serum of mice was collected regularly, and the levels of 5-HT and CORT in the serum of mice were detected using an enzyme-linked immunosorbent assay kit; S1-3: Compare the results obtained in S1-1 and S1-2, and screen out a group of Chinese herbal compound compositions with the best ratio of effects.

2. The method for preparing the sponge layer of the anxiety-relieving traditional Chinese medicine compound composition according to claim 1, characterized in that: The optimal group of Chinese herbal compound compositions screened out in step S1-3 is: 10g of Patchouli, 10g of Artemisia argyi, 15g of Mentha, 15g of Acorus calamus, 20g of Atractylodes, 15g of Ligusticum chuanxiong, and 15g of raw Pollen Typhae.

3. A sponge layer of a Chinese medicine compound composition for relieving anxiety prepared by the method of claim 1 or 2, characterized in that: The thickness is 1-3 mm. The filtrate of the freeze-dried Chinese medicine compound composition is uniformly filled in the porous structure of the sponge layer carrier, and the effective components of the filtrate Chinese medicine compound composition are slowly released along with the air flow passing through the porous structure.

4. A Chinese medicine compound mask for relieving anxiety, comprising a mask sheet and ear loops, characterized in that: The mask sheet is a multi-layer structure, and comprises, from the outside to the inside: an outer non-woven fabric layer, a graphene sponge layer, a Chinese medicine compound composition sponge layer as claimed in claim 3, and an inner non-woven fabric layer; wherein the graphene sponge layer and the Chinese medicine compound composition sponge layer both have wavy cross-sections, and the two sponge layers are parallel to each other, spaced apart, and have gaps between them.

5. The Chinese medicine compound composition mask for alleviating anxiety according to claim 4, characterized in that: The graphene sponge layer is prepared by the following method: A1: Preparation of mixed solution: carboxylated chitosan and gelatin were weighed in a mass ratio of 1:1, dissolved in deionized water to prepare a mixed solution with a mass concentration of 2%, heated to 37°C in a water bath, swollen for 24 hours, then 0.25wt% of genipin and 3wt% of glycerol were added and magnetically stirred for 30 minutes to obtain a gelatin mixed solution; A2: Low temperature freezing: Pour the gelatin mixed solution into a layered molding mold, freeze it in a low temperature refrigerator for 24 hours, and then freeze dry it in a freeze dryer for 48 hours to obtain a sponge layer carrier; A3: Immerse the sponge layer carrier obtained in step A2 into a graphene oxide solution with a concentration of 10 μg / mL, let it stand for a long time to allow the sponge to absorb the solution to saturation, and then freeze-dry it again to obtain a graphene sponge layer that relieves anxiety.

6. The anxiety-relieving Chinese medicinal compound mask according to claim 4, characterized in that: In the Chinese medicine compound sponge layer and the graphene sponge layer, the porosity of the sponge is 75%-85%.

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