An insecticide composition containing isoflualanam and its application

Abstract

The present invention belongs to the technical field of pesticide insecticides and discloses an insecticidal composition containing isoflualanam and its use. The active ingredients of the insecticidal composition include active ingredient A and active ingredient B, wherein active ingredient A is isoflualanam and active ingredient B is either avermectin or emamectin benzoate. The mass ratio of active ingredient A to active ingredient B is 1:32 to 45:1. The insecticidal composition of the present invention has excellent pest control effects and can be used for the integrated control of agricultural, horticultural, and forestry pests.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide killing, and discloses an insecticide composition containing isoflualanam and its application Background Art

[0002] Abamectin belongs to the class of macrolide disaccharide biopesticides, a broad-spectrum insecticide and acaricide effective against a wide range of pests, including those in the Lepidoptera, Coleoptera, and Hemiptera orders. Emamectin benzoate, also known as emamectin benzoate, is a new insecticide synthesized from avermectin B1. Both agents offer advantages such as high efficacy, low toxicity, and easy degradation. However, with widespread use of these two agents, many pests have developed resistance to them.

[0003] To address the above issues, a compounding of agents with different insecticide mechanisms from emamectin benzoate and abamectin can be selected, which not only improves drug efficacy and reduces costs, but is also one of the important methods for overcoming the development of pest resistance. Therefore, the inventors explored the combined effects of abamectin, emamectin benzoate and isoflualanam on various pests, aiming to provide a basis for the rational use of the above two biological insecticides and the coordination of chemical control and environmental protection. However, there are currently no reports on the control of pests by compounding isoflualanam with abamectin or emamectin benzoate and their combinations. Summary of the Invention

[0004] To address the above-mentioned problems in the prior art, the present invention provides an insecticidal composition containing isoflualanam and its application. The insecticidal composition has a significant synergistic effect on various lepidopteran and thymus-fever pests in agriculture, horticulture, and forestry, thereby improving the control effect and reducing the amount of pesticide used.

[0005] In order to achieve the above object, the present invention adopts the following technical solution: an insecticidal composition containing isoflualanam, wherein the active ingredients of the insecticidal composition include active ingredient A and active ingredient B, the active ingredient A is isoflualanam, the active ingredient B is either abamectin or emamectin benzoate, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 45:1.

[0006] Furthermore, the mass ratio of isoflualanam to avermectin is 1:32 to 35:1, or any value within the above numerical range; the mass ratio of isoflualanam to emamectin benzoate is 1:20 to 35:1, or any value within the above numerical range;

[0007] Furthermore, the mass ratio of isoflualanam to avermectin is 1:32 to 35:1, or any value within the above numerical range; the mass ratio of isoflualanam to emamectin benzoate is 1:20 to 32:1, or any value within the above numerical range;

[0008] Furthermore, the mass ratio of isoflualanam to avermectin is 1:22 to 20:1, or any value within the above numerical range; the mass ratio of isoflualanam to emamectin benzoate is 1:16 to 30:1, or any value within the above numerical range;

[0009] Furthermore, the total weight of the insecticide composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 80% of the total weight of the insecticide composition;

[0010] Furthermore, the insecticide composition contains, in addition to the active ingredient, auxiliary ingredients permitted in pesticides, wherein the auxiliary ingredients are selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier;

[0011] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0012] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0013] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0014] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0015] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0016] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0017] Defoaming agent selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0018] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0019] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0020] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0021] Synergists are selected from synergist, piperonyl butoxide; and / or

[0022] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0023] Furthermore, the insecticide composition is prepared in the form of a solid preparation or a liquid preparation;

[0024] The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0025] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions, or microcapsule suspension-suspoemulsions;

[0026] Furthermore, the solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is a suspension, an emulsifiable concentrate, a microemulsion, or an aqueous emulsion.

[0027] The present invention also discloses the use of the insecticide composition for preventing and controlling agricultural, horticultural and forestry pests.

[0028] Furthermore, the pests are Lepidoptera pests and Thysanoptera pests;

[0029] Furthermore, the Lepidoptera includes, but is not limited to, Plutellaxylostella, diamondback moth, Polychrosis viteana (grapeberry moth), Prays endocarpa, Prays oleae (olive moth), Pseudaletia spp. (snoctuids), Pseudaletia unipunctata (armyworm), Pseudoplusia includes (soybean looper), Rachiplusia nu, Scirpophaga incertulas, Sesamia spp. (stemborers), Sesamia inferens (pink rice stem borer), Sesamia nonagrioides), Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp. (snooper moths), Spodoptera exigua (beet armyworm), Spodoptera fugiperda (fall armyworm), Adoxophyes spp., Adoxophyes orana, Agrotis spp.(cutworm), Agrotis ipsilon (black cutworm), Alabama argillacea, Amorbia cuneana, Amyelosis transitella (navel orangeworm), Anarsia lineatella (peach twig borer), Anomissa bulifera (jute looper), Anticarsia gemma talis (velvet bean pelleter), Archips argyrospila (fruittree leafroller), Archips rosana (rose leaf roller), Argyrotaenia spp. (tortricidmoths), Argyrotaenia citrana, Autographa gamma, Bonagota cranaodes, Borbo cinnara), Caloptilia spp. (leaf miners), Capuareticulana, Carposina niponensis (peach fruit moth), Chilospp., Chlumetia transversa (mango shoot borer), Choristoneura rosaceana (obliquebanded leafroller), Chrysodeixis spp., Cnaphalocerus medinalis (grass leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus, Crambus spp.) (Sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydia nignicana (pea moth), Cydia pomonella, Darna diducta, Diaphania spp. (stem borers), Diatraea spp. (stalk borers), Diatraea saccharalis (sugarcane borer), Diatraea graniosella (southwester cornborer), Earias spp. (cotton bollworms), Earias spp. (Egyptian bollworms). insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopophaaurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Ephestia spp. (flour moths), Ephestia cautella (almond moth), Ephestia elutella (tobbaco moth), Ephestia kuehniella (Mediterranean flour moth), Epimeces spp., Epinotia aporema, Erionota thrax (banana skipper), Eupoecilia ambiguella, Euxoa auxiliaris (army cutworm), Feltia spp., Gortyna spp.(stemborers), Grapholita molesta (oriental fruit moth), Hedylepta indicate, bean leaf webber, Helicoverpas pp. (spotworms), Helicoverpa paarmigera, Helicoverpa zea, Heliothis spp. (spotworms), Heliothis virescens, tobacco budworm, Hellula undalis (cabbage webworm), Indarbela spp., root borers, Keiferia lycopersicella, (tomato pinworm), Leucinodes orbonalis (eggplant fruit borer). borer), Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (grape fruit moth), Loxagrotis spp. (southworm), Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), Malacosoma spp.(tent caterpillars), Mamestra brassicae (cabbage armyworm), Maruca testulalis, Metisa plana, Mythimna unipuncta (true armyworm), Neoleucinodes elegantalis (small tomato borer), Operophthera brumata (winter moth), Ostrinia nubilalis (European corn borer), Pandemis cerasana, common currant tortrix, Pandemis heparana (brown apple tortrix), Papilio demodocus, Pectinophora gossypiella (pink bollworm), Peridroma species ( spp. (cutworm), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis citrella, Phyllonorycter spp. (leafminers), Pieris rapae (imported cabbageworm), Plathypena scabra, Plodia interpunctella (Indian mealmoth), Spodoptera oridania (southern armyworm), Synanthedon spp.) (root borers), Thecla basilides, Thermisiagemmatalis, Tineola bisselliella (webbing clothes moth), Trichoplusia ni (rape worm), Tuta absoluta, Yponomeuta spp., Zeuzera coffeae (red branch borer), and Zeuzera pyrina (leopard moth).

[0030] The Thysanoptera pests include, but are not limited to, Frankliniella occidentalis, Thrips spp., Scirtothrips dorsalis, Stenchaetothrips biformis, Frankliniella intonsta, Thrips palmi, Anaphothrips obscurus, Neohydatothrips samayunkur, Dendrothrips minowai, Haplothrips aculeatus, Frankliniella tenuicornis, and Thrips hawaiiensis.

[0031] Furthermore, the lepidopteran pest is rice leaf folder, and the thysanopteran pest is thrips.

[0032] In order to obtain the desired insecticidal effect, the dosage of the insecticidal composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application site, application method, dosage form used, etc.

[0033] The beneficial effects of the present invention are as follows:

[0034] 1) The insecticidal composition of the present invention rationally combines compounds with different mechanisms of action, and has a significant synergistic effect on pests at a certain mass ratio;

[0035] 2) The insecticidal composition of the present invention has excellent control effects on Lepidoptera and Thysanoptera pests in agriculture, horticulture and forestry;

[0036] 3) The insecticide composition of the present invention reduces the amount of pesticides used, has less impact on the environment, and slows down the occurrence of pesticide resistance in pests. DETAILED DESCRIPTION

[0037] Preparation example:

[0038] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0039] Preparation example:

[0040] Preparation Example 1: 12% isoflualanam·avermectin suspension (1:2)

[0041] Formula: 4% isoflualanam, 8% avermectin, 2% sodium lauryl sulfate, 1% naphthalenesulfonate formaldehyde condensate, 2% arylphenol polyoxyethylene ether phosphate, 3% styrylphenol polyoxyethylene ether phosphate, 2% antioxidant BHT, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.1% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0042] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0043] Preparation Example 2: 1.8% isoflualanam·avermectin microemulsion (1:8)

[0044] Formula: 0.2% isoflualanam, 1.6% avermectin, 10% phenylethylphenol polyoxyethylene polyoxypropylene ether, 1% tristyrylphenol ethoxylate phosphate, 6% tristyrylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 18% cyclohexanone, 0.5% antioxidant BHT, 0.01% silicone defoaming agent, and deionized water to make up the balance;

[0045] Preparation method: After completely dissolving the active ingredient in the solvent according to the formula ratio, add an emulsifier to make an oil phase, add a dispersant, deionized water, etc. and stir evenly to make an aqueous phase; add the oil phase to the aqueous phase and stir evenly, shear at high speed until the particle size meets the requirements, add a defoaming agent and stir evenly to obtain the microemulsion product.

[0046] Preparation Example 3: 11% isoflualanam·avermectin emulsifiable concentrate (10:1)

[0047] Formula: 10% isoflualanam, 1% avermectin, 18% propylene glycol methyl ether, 12% sorbitan oleate polyoxyethylene ether, 4% calcium dodecylbenzenesulfonate, 0.5% antioxidant BHT, 10% DMF, 22% cyclohexanone, and methyl oleate makes up the balance.

[0048] Preparation method: According to the formula ratio, the measured active ingredients, solvent and cosolvent are added to a mixing kettle and stirred to dissolve them, then the emulsifier is added and the balance is supplemented with the remaining solvent, and the mixture is stirred evenly in a stirring kettle. After filtering, the desired emulsifiable concentrate of the present invention is obtained.

[0049] Preparation Example 4: 2.2% isoflualanam·avermectin aqueous emulsion (1:1)

[0050] Formula: 1.1% isoflualanam, 1.1% avermectin, 0.5% antioxidant BHT, 10% toluene, 5% dehydrated sorbitan polyoxyethylene ether, 10% acetophenone, 10% cyclohexanone, 1% fatty alcohol polyoxyethylene ether sodium sulfate, 0.25% xanthan gum, 4% glycerol, 0.1% benzoic acid, 0.25% polyoxypropylene polyoxyethylene glycerol ether, and deionized water to make up the balance.

[0051] Preparation method: According to the formula ratio, the active ingredient is added together with the solvent, emulsifier and cosolvent to dissolve into a uniform oil phase; part of the water, antifreeze agent and other pesticide adjuvants are mixed together to form a uniform water phase; while stirring at high speed in a reactor, the oil phase is added to the water phase, and after stirring evenly, a shearing machine is turned on for high-speed shearing, and the remaining water is added and sheared for about half an hour to form an oil-in-water emulsion.

[0052] Preparation Example 5: 16% isoflualanam·avermectin water dispersible granules (3:1)

[0053] Formula: 12% isoflualanam, 4% avermectin, 8% lignin sulfonate, 8% naphthalene sulfonate formaldehyde condensate, 3% sodium succinate sulfonate, 2% antioxidant BHT, 5% white carbon black, 30% starch, and kaolin makes up the balance.

[0054] Preparation method: According to the formula ratio, the active ingredient is added to the carrier, and surfactants and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then kneading, granulation, drying, and screening are carried out to obtain a water-dispersible granule product; or the crushed powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0055] Preparation Example 6: 16.8% isoflualanam·avermectin wettable powder (1:3)

[0056] Formula: 4.2% isoflualanam, 12.6% avermectin, 10% sodium lignin sulfonate, 2% sodium lauryl sulfate, 3% alkylphenol formaldehyde resin polyoxyethylene ether sulfate, 2% antioxidant BHT, 5% white carbon black, 28% starch, and kaolin makes up the balance;

[0057] Preparation method: According to the formula ratio, the active ingredients, dispersants, wetting agents and fillers are mixed, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0058] Preparation Example 7: 10% isoflualanam·emamectin benzoate suspension (3:2)

[0059] Formula: 6% isoflualanam, 4% emamectin benzoate, 2% antioxidant BHT, 2% sorbitan oleate polyoxyethylene ether, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 2% styrenated phenol polyoxyethylene ether phosphate, 1% sodium salt of polycarboxylate, 1.5% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% fumed silica, 1% sodium sorbate, 5% glycerol, 0.5% silicone oil, and deionized water to make up the balance;

[0060] Preparation method: Same as Preparation Example 1.

[0061] Preparation Example 8: 4.5% isoflualanam·emamectin benzoate microemulsion (1:8)

[0062] Formula: 0.5% isoflualanam, 4% emamectin benzoate, 2% antioxidant BHT, 16% xylene, 20% cyclohexanone, 15% castor oil polyoxyethylene ether, 5% EO-PO block copolymer, 2% styrenated phenol polyoxyethylene ether sulfate, 5% ethylene glycol, 0.1% silicone defoamer, and deionized water to make up the balance;

[0063] Preparation method: Same as Preparation Example 2.

[0064] Preparation Example 9: 20% isoflualanam·emamectin benzoate emulsifiable concentrate (4:1)

[0065] Formula: 16% isoflualanam, 4% emamectin benzoate, 2% antioxidant BHT, 15% DMF, 15% glycerol fatty acid ester polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 18% propylene carbonate, and xylene makes up the balance.

[0066] Preparation method: Same as Preparation Example 3.

[0067] Preparation Example 10: 4.5% isoflualanam·emamectin benzoate aqueous emulsion (8:1)

[0068] Formula: 4% isoflualanam, 0.5% emamectin benzoate, 0.2% antioxidant BHT, 2% polyoxyethylene sorbitan monooleate, 6% Guerbet alcohol polyoxyethylene ether, 10% styrylphenol polyoxyethylene ether, 1% calcium dodecylbenzenesulfonate, 20% cyclohexanone, 5% ethylene glycol, 0.05% silicone defoamer, and deionized water to make up the balance;

[0069] Preparation method: same as Preparation Example 4.

[0070] Preparation Example 11: 17% isoflualanam·emamectin benzoate water dispersible granules (1:16)

[0071] Formula: 1% isoflualanam, 16% emamectin benzoate, 3% antioxidant BHT, 5% succinate sulfonate, 3% BX powder, 5% sodium lignin sulfonate, 8% sodium polycarboxylate, 5% white sugar, kaolin makes up the balance;

[0072] Preparation method: Same as Preparation Example 5.

[0073] Preparation Example 12: 16% isoflualanam·emamectin benzoate wettable powder (15:1)

[0074] Formula: 15% isoflualanam, 1% emamectin benzoate, 0.5% antioxidant BHT, 5% sodium lignin sulfonate, 5% dispersant NNO, 2% opening powder BX, 5% white carbon black, kaolin makes up the balance;

[0075] Preparation method: Same as Preparation Example 6.

[0076] Example 1: Indoor biological activity test on lepidopteran pests

[0077] Test basis: The test refers to NY / T-1154.14-2008 "Guidelines for Indoor Biological Testing of Pesticides - Insecticides Part 14: Leaf Dipping Method".

[0078] Test target: 3rd instar larvae of rice leaf folder.

[0079] Test drugs: isoflualanam, avermectin, and emamectin benzoate technical.

[0080] Preparation of test agents: After dissolving the above agents in appropriate solvents, dilute them to appropriate concentrations with water containing 0.1% Tween-80, and use a treatment without agents (containing all organic solvents and emulsifiers) as a blank control.

[0081] Test Method: A rice leaf immersion method was used. Fresh, untreated rice leaves were immersed in each treatment solution for 20 seconds. After removal and air drying at room temperature, the lower portion of the leaves were wrapped with soaked absorbent cotton and placed in a culture cup, with six leaves per cup. Ten third-instar larvae of the rice leaf roller were then inoculated and sealed with gauze. Each treatment was replicated four times. The treated insects were placed in an artificial climate chamber at (26 ± 1)°C with a photoperiod of L:D = 16h:8h.

[0082] Experimental investigation: 72 hours after treatment, the number of dead insects in each treatment was investigated. The criterion for death was that the insect body could not coordinate movement when the insect body was lightly touched with a brush.

[0083] Calculation method:

[0084] Based on the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0085]

[0086] Where:

[0087] P——mortality rate, in percentage (%);

[0088] K——indicates the number of dead insects, the unit is head;

[0089] N——represents the total number of insects processed, in heads.

[0090]

[0091] Where:

[0092] P1——adjusted mortality rate, in percentage (%);

[0093] P t ——Treatment mortality rate, expressed in percentage (%);

[0094] P0 - blank control mortality rate, in percentage (%).

[0095] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0096] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0097] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0098]

[0099] Where:

[0100] ATI - measured toxicity index of mixture;

[0101] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0102] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0103] TTI=TI A ×P A +TI B ×P B

[0104] Where:

[0105] TTI – Theoretical Toxicity Index of Mixtures;

[0106] TI A ——Agent toxicity index;

[0107] P A ——The percentage of agent A in the mixture, in percentage (%);

[0108] TI B ——Toxicity index of agent B;

[0109] P B ——The percentage of agent B in the mixture, in percentage (%).

[0110]

[0111] Where:

[0112] CTC – Co-toxicity coefficient;

[0113] ATI - measured toxicity index of mixture;

[0114] TTI - Theoretical Toxicity Index of Mixture.

[0115] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0116] The indoor test results are shown in the table below:

[0117] Table 1 Results of indoor biological activity test of Isoflualanam and Abamectin against rice leaf roller

[0118]

[0119] The results of indoor bioactivity tests (see Table 1) show that the combination of isoflualanam and avermectin at appropriate mass ratios is highly effective against the lepidopteran pest, Cnaphalocrocis medinalis. When the mass ratio of isoflualanam to avermectin was 1:24 to 35:1, the co-toxicity coefficient against Cnaphalocrocis medinalis was greater than 120, indicating a synergistic effect. When the mass ratio of isoflualanam to avermectin was 1:16 to 26:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect. When the mass ratio of isoflualanam to avermectin was 1:16 to 18:1, the co-toxicity coefficient was greater than 140, indicating a significant synergistic effect.

[0120] Table 2 Results of indoor biological activity test of Isoflualanam and emamectin benzoate against rice leaf roller

[0121]

[0122]

[0123] The results of indoor biological activity tests (Table 2) show that the combination of isoflualanam and emamectin benzoate has significant control effects against the rice leaf folder at appropriate mass ratios. The mass ratio of isoflualanam to emamectin benzoate was 1:20 to 35:1, and the co-toxicity coefficient against the lepidopteran pest, Cnaphalocrocis medinalis, was greater than 120, indicating a synergistic effect. The mass ratio of isoflualanam to emamectin benzoate was 1:16 to 30:1, with a co-toxicity coefficient greater than 130, indicating a significant synergistic effect. The mass ratio of isoflualanam to emamectin benzoate was 1:8 to 25:1, with a co-toxicity coefficient greater than 140, indicating a significant synergistic effect.

[0124] Example 2: Indoor biological activity test against Thysanoptera pests

[0125] Test target: Adult western flower thrips.

[0126] Test agents: compound of formula I, avermectin, and avermectin benzoate technical.

[0127] Preparation of drugs: After dissolving the above drugs in appropriate solvents, dilute them to appropriate concentrations with water containing 0.1% Tween-80. Use the treatment without drugs (containing all organic solvents and emulsifiers) as a blank control.

[0128] Experimental Method: The test used the leaf tube film method. Each concentration of the drug solution was filled into a 1.5 mL centrifuge tube. The tubes were placed on a bench to dry. A 2-3 mm hole was punched in the bottom of the tube using a fine needle. Each tube served as a replicate, and each concentration was repeated four times. A tube immersed in clean water served as a control. Fresh cabbage leaves were punched into circular pieces with a diameter of 1 cm using a hole punch. The leaves were immersed in each concentration of the drug solution for 10 seconds. A blank control was also immersed in clean water. The immersed leaves were removed, dried, and placed in centrifuge tubes containing the corresponding drug solution concentration, with one leaf per tube. Test insects were placed in the centrifuge tubes using a sucker, with 10 test insects per replicate. The tubes were capped and the holes in the bottom of the tubes were sealed with parafilm. The treated insects were placed in an artificial climate chamber at 25°C with a light cycle of L:D = 16h:8h.

[0129] Experimental investigation: The mortality rate was checked after 48 hours. The criterion for judging death was that if the insect crawled no more than one insect length when lightly touched with the tip of a brush, it was considered dead.

[0130] Calculation method:

[0131] Based on the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0132]

[0133] Where:

[0134] P——mortality rate, in percentage (%);

[0135] K——indicates the number of dead insects, the unit is head;

[0136] N——represents the total number of insects processed, in heads.

[0137]

[0138] Where:

[0139] P1——adjusted mortality rate, in percentage (%);

[0140] P t ——Treatment mortality rate, expressed in percentage (%);

[0141] P0 - blank control mortality rate, in percentage (%).

[0142] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0143] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50The activity of the test agent on the biological test material is evaluated by the value.

[0144] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0145]

[0146] Where:

[0147] ATI - measured toxicity index of mixture;

[0148] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0149] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0150] TTI=TI A ×P A +TI B ×P B

[0151] Where:

[0152] TTI – Theoretical Toxicity Index of Mixtures;

[0153] TI A ——Agent toxicity index;

[0154] P A ——The percentage of agent A in the mixture, in percentage (%);

[0155] TI B ——Toxicity index of agent B;

[0156] P B ——The percentage of agent B in the mixture, in percentage (%).

[0157]

[0158] Where:

[0159] CTC – Co-toxicity coefficient;

[0160] ATI - measured toxicity index of mixture;

[0161] TTI - Theoretical Toxicity Index of Mixture.

[0162] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0163] The indoor test results are shown in the table below:

[0164] Table 3 Results of indoor biological activity test on thrips by combining Isoflualanam and avermectin

[0165]

[0166] The results of the indoor bioactivity tests (see Table 3) show that isoflualanam and avermectin, when combined at appropriate mass ratios, are highly effective against the Thysanoptera pest thrips. The mass ratio of isoflualanam to avermectin was 1:32 to 35:1, and the co-toxicity coefficient against thrips was greater than 120, indicating a synergistic effect. The mass ratio of isoflualanam to avermectin was 1:22 to 20:1, and the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect. The mass ratio of isoflualanam to avermectin was 1:15 to 10:1, and the co-toxicity coefficient was greater than 140, indicating a significant synergistic effect.

[0167] Table 4 Results of indoor biological activity test on thrips with Isoflualanam and emamectin benzoate

[0168]

[0169] The results of the indoor bioactivity tests (Table 4) show that isoflualanam and emamectin benzoate, when combined in appropriate mass ratios, exhibit significant control effects against the Thysanoptera pest thrips. The mass ratios of isoflualanam to emamectin benzoate were 1:20 to 32:1, and the cotoxicity coefficient against thrips was greater than 120, indicating a synergistic effect. The mass ratios of isoflualanam to emamectin benzoate were 1:16 to 24:1, and the cotoxicity coefficients were greater than 130, indicating a significant synergistic effect. The mass ratios of isoflualanam to emamectin benzoate were 1:8 to 16:1, and the cotoxicity coefficients were greater than 140, indicating a significant synergistic effect.

[0170] Example 3: Field efficacy test of mixed pesticides against rice leaf roller

[0171] Test basis: The test refers to GB / T 17980.2-2000 "Guidelines for field efficacy tests of pesticides (I) Insecticides for control of rice leaf folders".

[0172] Site Overview: The experiment was conducted in rice paddies in Ganquan Town, Yangzhou City, Jiangsu Province. The previous crop was wheat, and rice leaf rollers are a frequent problem. The soil fertility at the site is medium, with sandy loam and excellent irrigation and drainage facilities, resulting in excellent rice growth. Cultivation conditions and fertilizer and water management were consistent across all plots.

[0173] Test object: Rice leaf roller.

[0174] Experimental design: The experiment was conducted in 8 treatments, with 4 replicates per treatment, a total of 32 plots, and a plot area of ​​20 m 2 The plots are arranged in random blocks, and small ridges are built between the plots to prevent water from flowing through.

[0175] Application time: The test was conducted by applying pesticide once when the rice leaf roller was in the 1st to 2nd instar larvae. The application equipment was Linong-HD400 backpack sprayer. The pesticide dosage per hectare was 750 kg diluted with water and sprayed evenly.

[0176] Experimental investigation: The number of curled leaves in each treatment was investigated 7 and 14 days after application. A five-point sampling method was used, with five rice clumps surveyed at each point. The total number of leaves surveyed and the number of curled leaves were recorded, and the curl rate and control efficacy were calculated.

[0177] Calculation method of drug efficacy:

[0178]

[0179] The results of the field efficacy test are shown below:

[0180] Table 5 Field efficacy test results of mixed pesticides against rice leaf roller

[0181]

[0182] As shown in Table 5, the combined compositions exhibited both rapid and persistent effectiveness against rice leaf rollers. Seven days after application, leaf curl rates were 1.12%, 0.94%, 0.88%, and 0.71% for the 9% isoflualanam·emamectin benzoate EW (8:1), 10% isoflualanam·emamectin benzoate SC (3:2), 22% isoflualanam·avermectin EW (1:1), and 16% isoflualanam·avermectin WDG (3:1), respectively. Fourteen days after application, the control efficacy of the combined formulations was significantly enhanced.

[0183] Example 4: Field efficacy test of mixed pesticides against pepper thrips

[0184] Test basis: The test refers to NY / T 1464.6-2007 "Guidelines for Field Efficacy Tests of Pesticides Part 6: Insecticides for Control of Vegetable Thrips".

[0185] Experimental location: The experiment was conducted in the pepper fields of Sancun, Baixiang, Liuwang Town, Jiaonan City, Shandong Province. The soil fertility of the experimental site was medium, which was in line with local scientific agricultural practices.

[0186] Test target: Thrips (mixed populations of Thrips palmi and Thrips occidentalis).

[0187] Test crop: Pepper (Caesar).

[0188] Experimental design: The experiment set up 5 pesticide treatments, with water spraying as a blank control, and each treatment was repeated 4 times. No other pesticides were used before spraying the test pesticides, and the peppers in all test plots grew uniformly. The plots were arranged randomly, with protective rows added around the plots, and each plot was 20m2. 2 .

[0189] Test method: The test was conducted on May 10, 2024. The pesticide was applied once using a Gongnong-16 backpack sprayer. The plants were evenly sprayed with the pesticide.

[0190] Survey Methods: Survey the base population before application and the number of surviving insects 3 and 7 days after application. Sampling was conducted at five locations per plot, with four pepper plants fixed at each location. Five flowers were randomly selected from each pepper plant, and the number of thrips present was recorded to calculate control efficacy.

[0191] Calculation method of drug efficacy:

[0192]

[0193] Field efficacy test results:

[0194] Table 6 Field efficacy test results of mixed pesticides against pepper thrips

[0195]

[0196]

[0197] The test results show (Table 6) that the combination of isoflualanam with abamectin and emamectin benzoate for the control of thrips has good fast-acting effect and a long lasting effect.

[0198] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is limited by the appended claims and their legal equivalents.

Claims

1. An application of an isoflualanam-containing insecticidal composition for controlling agricultural, horticultural, and forestry pests, characterized in that: The active ingredients of the insecticide composition include active ingredient A and active ingredient B, wherein the active ingredient A is isoflualanam, and the active ingredient B is either abamectin or emamectin benzoate. The mass ratio of isoflualanam to abamectin is 1:24-35:1; the mass ratio of isoflualanam to emamectin benzoate is 1:20-35:1, and the pest is rice leaf roller.

2. The use according to claim 1, characterized in that The mass ratio of isoflualanam to avermectin is 1:16-26:1; the mass ratio of isoflualanam to emamectin benzoate is 1:16-30:

1.

3. The use according to claim 2, characterized in that The mass ratio of isoflualanam to avermectin is 1:16-18:1; the mass ratio of isoflualanam to emamectin benzoate is 1:8-25:

1.

4. The use according to claim 1, characterized in that The total weight of the insecticide composition is 100 wt %, and the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 80% of the total weight of the insecticide composition.

5. The use according to claim 1, characterized in that In addition to the active ingredients, the insecticide composition also contains auxiliary ingredients allowed in pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

6. The use according to claim 1, characterized in that The insecticide composition is prepared in the form of a solid preparation or a liquid preparation.

7. The use according to claim 6, characterized in that The solid preparations are water-dispersible granules and wettable powders, and the liquid preparations are suspensions, emulsifiable concentrates, microemulsions, and aqueous emulsions.

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