Odor-free and refreshing deodorant lotion and preparation method thereof

Abstract

The present application provides a clean and refreshing deodorant and a preparation method thereof. The clean and refreshing deodorant of the present application comprises ingredients such as witch hazel extract, aluminum chlorohydrate, alum, aluminum sulfate, hexylene glycol, p-hydroxyacetophenone, triethyl citrate, propylene glycol, farnesol, a solubilizer and essence, among which the enzymatically hydrolyzed witch hazel extract can provide better antiperspirant and deodorizing effects.

Description

Field of the Invention

[0001] The present application belongs to the field of cosmetics or skin disease treatment drugs. Specifically, the present application provides a clean and refreshing deodorant lotion and a preparation method thereof. Background Art

[0002] Sweat secretion is a normal physiological process of the human body, but in some individuals or groups, the sweat glands are overdeveloped. After exercise, in a hot or poorly ventilated environment, excessive sweat and secretions from the surrounding sebaceous glands can easily breed bacteria, causing problems such as sweat odor or body odor. These problems seriously affect the patient's normal social life, and this group has a continuous demand for antiperspirant and deodorant products.

[0003] At present, the commonly used ingredients of antiperspirant and deodorant products include aluminum chlorohydrate, alum, farnesol, etc. The efficacy of products using these ingredients is still insufficient. There is a need in the field to develop safe and effective new antiperspirant and deodorant ingredients and odor-free deodorant products.

[0004] Witch hazel is a plant of the genus Hamamelis native to North America. Native Americans have a long history of using witch hazel bark and leaves to treat skin and oral diseases. Witch hazel extracts and distillates are rich in tannic acid, gallic acid, catechins, proanthocyanidins, flavonoids and other ingredients, which provide clear anti-inflammatory, antioxidant, astringent, and antipruritic effects. Therefore, witch hazel extracts are currently a common ingredient in cosmetics and skin medicines. Summary of the invention

[0005] On the one hand, the present application provides a clean and refreshing deodorant, which contains witch hazel extract, aluminum chlorohydrate, alum, and aluminum sulfate.

[0006] Furthermore, the clean and refreshing deodorant lotion also contains hexylene glycol, p-hydroxyacetophenone, triethyl citrate, propylene glycol, farnesol, a solubilizer and a fragrance.

[0007] Furthermore, the compatibilizer is CO-40.

[0008] Furthermore, the content of the witch hazel extract in the odor-free and refreshing deodorant is 2-5% v / v.

[0009] Furthermore, the content of witch hazel extract in the odor-free and refreshing deodorant is 3% v / v.

[0010] Furthermore, the preparation method of the Hamamelis Virginiana extract comprises:

[0011] (1) crushing witch hazel leaves into powder;

[0012] (2) adding the powder obtained in step (1) to a 15-30% v / v ethanol aqueous solution having a mass of 5-8 times that of the powder, adding a protease and an emulsifier to the aqueous solution, and mixing well;

[0013] (3) Soak at 25-35°C for 12-16 hours, stirring for 1-3 minutes every 15-30 minutes;

[0014] (4) placing at 85-95° C. for 5-10 minutes to inactivate the enzyme, and cooling to obtain a witch hazel extract.

[0015] Furthermore, the protease is ficin, and the emulsifier is Span 20.

[0016] Furthermore, step (2) is: adding the powder obtained in step (1) into a 15% v / v ethanol aqueous solution which is 6 times the mass of the powder, adding 800 U / mL of ficin and 0.5% w / v of Span 20 into the aqueous solution, and mixing well.

[0017] Furthermore, in step (3), the mixture is immersed at 30° C. for 12 hours.

[0018] Furthermore, in step (4), the enzyme is inactivated at 90° C. for 8 minutes.

[0019] On the other hand, the present application provides the use of the above-mentioned odor-free and refreshing deodorant lotion in the preparation of cosmetics or medicines with antiperspirant and deodorizing functions.

[0020] The clean and refreshing deodorant lotion of the present application effectively enhances the antiperspirant and deodorant effect of the product by scientifically combining several known antiperspirant and deodorant ingredients on the one hand and enzymatically improving the North American witch hazel extract on the other hand. The ingredients used in the preparation process are all types that can be used in food / cosmetics, which effectively ensures the safety of the product. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is the average number of sweat points of rats in each group in Example 2;

[0022] Figure 2 is the average number of sweat points of rats in each group in Example 3;

[0023] Figure 3 This is a comparison chart of the effects of the odor-free and refreshing deodorant lotion and the Ban Yue Qing deodorant. DETAILED DESCRIPTION

[0024] raw material:

[0025] Imported North American witch hazel bark, twigs and leaves are provided by qualified cosmetic raw material suppliers and are manually selected to remove impurities and poor quality parts.

[0026] Experimental animals SD rats were purchased from the Animal Experiment Center of Wuhan University.

[0027] Reserpine injection: Tianjin Jinyao.

[0028] The Wada-Takagaki reagent was homemade: Liquid A was an anhydrous ethanol solution containing 2% iodine, and Liquid B was 50 g of soluble starch mixed in 100 mL of vegetable oil.

[0029] Other reagents are all domestic cosmetic / food grade.

[0030] Example 1 Basic preparation method of Hamamelis Virginiana extract

[0031] The applicant has used witch hazel extract in previous research and products. To further improve the effect, the applicant tried to enzymatically improve the natural composite ingredients in the product, witch hazel extract. According to the prior art, witch hazel, especially the water or alcohol extracts of witch hazel branches and leaves, contain tannic acid, gallic acid, catechins, proanthocyanidins, flavonoids, as well as some water-soluble polysaccharides and protein components. Current research has little research on these protein or polysaccharide components. The applicant tried to enzymatically hydrolyze these protein or polysaccharide components to further improve the antiperspirant and deodorant effect of witch hazel extract.

[0032] The basic extraction method is:

[0033] (1) crushing witch hazel leaves into powder;

[0034] (2) adding the powder obtained in step (1) to a 15-30% v / v ethanol aqueous solution having a mass of 5-8 times that of the powder, adding protease to the aqueous solution, and mixing well;

[0035] (3) Soak at 25-35°C for 12-16 hours, stirring for 1-3 minutes every 15-30 minutes;

[0036] (4) placing at 85-95° C. for 5-10 minutes to inactivate the enzyme, and cooling to obtain a witch hazel extract.

[0037] The enzymatic hydrolysis schemes attempted included the following proteases and polysaccharidase. Considering that 15% ethanol may cause some loss of enzyme activity, the enzyme dosage was appropriately increased based on similar methods.

[0038] Table 1 Enzymatic hydrolysis scheme

[0039] Group Enzymatic hydrolysis protocol Group 1 Papain 800U / mL Group 2 Bromelain 800U / mL Group 3 Ficin 800U / mL Group 4 Trypsin 800U / mL Group 5 Cellulase 1000U / mL Group 6 Pectinase 1000U / mL Group 7 Cellobiase 1000U / mL Group 8 No enzyme modification .

[0040] Example 2 Effect of Hamamelis Virginiana Extract on Sweating in Rats

[0041] With reference to the prior art, a spleen deficiency and sweating rat model was constructed using reserpine injection, and the Hotan-Takagaki reagent was used to detect the sweating of experimental animals.

[0042] The specific process is as follows:

[0043] A total of 100 male SD rats were used and randomly divided into 10 groups. After adaptive feeding, except for the normal control group, the other 9 groups were given 0.2 mg / kg / d of reserpine subcutaneously in the abdomen for 7 consecutive days (the normal control group was given an equal amount of distilled water);

[0044] The first injection day was defined as day 1. On days 5, 6, and 7, 8 of the 9 groups of rats that received reserpine were given the drugs of groups 1 to 8 in Example 1 (applied to the entire hind paw and the surrounding area), counted as groups 1 to 8; group 9 was a model control group that received reserpine and then applied distilled water to the hind paw; group 10 was a normal control group that was not given reserpine;

[0045] On the eighth day, the rats' hind feet and surrounding parts were washed and dried with clean water, and then placed in a closed electric running wheel for forced jogging for 3 minutes (running wheel diameter 40 cm, speed 20 rpm);

[0046] Fix the rat, apply Hotan-Takagaki reagent A solution to the rat's paw skin, and after it dries naturally, apply B solution. Wait for 15 minutes, and use a magnifying glass to observe and count the purple sweat spots.

[0047] The results are as follows Figure 1 As shown: as compared with Group 9 and Group 10, the administration of reserpine effectively amplified the sweating of rats; Hamamelis virginiana extract does have the effect of regulating and inhibiting sweat glands, enzymatic hydrolysis by polysaccharidase does not help to enhance this effect, and enzymatic hydrolysis by ficin and papain slightly improves the antiperspirant effect.

[0048] Example 3 Further Improvement of Enzymatic Hydrolysis Process

[0049] The applicant attempted to use emulsifiers during enzymatic hydrolysis to change the existence form of protein peptide chains and sugar chains to enhance the effect of enzymatic hydrolysis.

[0050] Table 2 Improved enzymatic hydrolysis scheme

[0051]

[0052]

[0053] The rat sweating experiment was conducted again according to the description of Example 2, in which the original protocols of Group 1, Group 3, Group 9, and Group 10 were repeated.

[0054] The results are as follows Figure 2As shown, the addition of emulsifier had little effect on the papain enzymatic group, while Span 20 significantly improved the effect of the fig protease enzymatic group, and the number of sweating points was less than half of that in the model control group.

[0055] Example 4: Fresh and clean deodorant product and its application

[0056] The applicant prepared an actual odor-free and refreshing deodorant product with the enzymatically hydrolyzed witch hazel extract of Group 3-1 in the above Example 3, comprising: 3% v / v witch hazel extract, 30% v / v aluminum chlorohydrate (50%), 21% v / v alum (10%), 3% v / v aluminum sulfate (30%), 0.5% v / v farnesol, and appropriate amounts of antibacterial agent hexylene glycol, antioxidant p-hydroxyacetophenone, enzyme inhibitor triethyl citrate, solvent water and propylene glycol, solubilizer CO-40 and fragrance.

[0057] A number of testers with a keen sense of smell and without any influencing diseases such as rhinitis were selected from the applicant's company. They were trained to score using an aqueous solution of isovaleric acid. After about one week of contact, the testers were able to accurately judge the concentration of the aqueous solution of isovaleric acid (the accuracy rate of each of the five selected testers was greater than 95%).

[0058] Table 3 Relationship between concentration and fraction of isovaleric acid aqueous solution

[0059]

[0060]

[0061] 20 male testers with obvious sweat odor problems in the armpits were selected from the applicant's supplier-related companies, and the left and right armpits were fixed for testing. The testers used unscented soap to wash their armpits several times before going to bed, and applied cotton armpit sweat pads to their armpits. After 12 hours, they were removed and sealed, and then handed over to the judges for scoring (according to the above-mentioned isovaleric acid aqueous solution, a score of 1-5 was given, without decimals; the average of each case was taken after 3 consecutive tests).

[0062] The 20 test subjects were randomly divided into 2 groups and given the above-mentioned refreshing deodorant and the commercially available Ban Yue Qing Armpit Deodorant (Yibao brand) respectively. The average scores before and after using the drug were as follows: Figure 3 As shown, the results show that the refreshing deodorant has a definite antiperspirant and deodorizing effect, and the effect is better than the commonly used Banyueqing medicine.

Claims

1. A clean and refreshing deodorant, characterized in that: The clean and refreshing deodorant contains witch hazel extract, aluminum chlorohydrate, alum and aluminum sulfate; The witch hazel extract is prepared by the following method: (1) crushing witch hazel leaves into powder; (2) adding the powder obtained in step (1) into a 15% v / v ethanol aqueous solution of 6 times the mass of the powder, adding 800 U / mL of ficin and 0.5% w / v of Span 20 into the aqueous solution, and mixing well; (3) Soak at 25-35°C for 12-16 hours, stirring for 1-3 minutes every 15-30 minutes; (4) placing at 85-95° C. for 5-10 minutes to inactivate the enzyme, and cooling to obtain a witch hazel extract.

2. The clean and refreshing deodorant lotion according to claim 1, further comprising hexylene glycol, p-hydroxyacetophenone, triethyl citrate, propylene glycol, farnesol, a solubilizer and a fragrance.

3. The odor-free and refreshing deodorant lotion according to claim 2, wherein the solubilizing agent is CO-40.

4. The odor-free and refreshing deodorant lotion according to claim 1, wherein the content of the witch hazel extract in the odor-free and refreshing deodorant lotion is 2-5% v / v.

5. The odor-free and refreshing deodorant lotion according to claim 4, wherein the content of the witch hazel extract in the odor-free and refreshing deodorant lotion is 3% v / v.

6. The odor-free and refreshing deodorant according to claim 1, wherein in step (3) of the method for preparing the witch hazel extract, the mixture is immersed at 30°C for 12 hours, and in step (4), the enzyme is inactivated at 90°C for 8 minutes.

7. Use of the odor-free and refreshing deodorant lotion according to any one of claims 1 to 6 in the preparation of cosmetics or medicines with antiperspirant and deodorizing functions.

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