A method for quickly screening high-embryo materials of radish

By quantitative analysis of the fluorescence of the gene expression of ENHANCER OF SHOOT of the radish variety, the problem of efficient screening of high embryo-out materials in radish microspore culture was solved, and the microspore embryo-out rate was quickly and stably improved.

CN119040505BActive Publication Date: 2025-07-22ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202411272490.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-09-11
Publication Date
2025-07-22
Estimated Expiration
2044-09-11

AI Technical Summary

Technical Problem

In the prior art, haploid embryo induction efficiency during the culture of radish microspores is low, and it is difficult to quickly screen out high embryo materials, especially the culture effect of radish varieties is not significant.

Method used

By quantitative analysis of the expression of ENHANCER OF SHOOT gene among different radish varieties, the radish varieties with the lowest ENHANCER OF SHOOT gene expression were screened as high embryo materials. Fluorescence quantitative PCR technology was used to detect gene expression and screen radish materials with high embryo rates were screened.

Benefits of technology

It realizes rapid and effective screening of radish materials with high embryo yield, improves the efficiency of microspore culture, saves screening time and manpower, and the obtained materials are stable and widely adaptable.

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Abstract

The present invention relates to the technical field of plant cultivation, and provides a method for rapidly screening high-embryo-rate radish materials. The method screens high-embryo-rate materials by analyzing the expression levels of the ENHANCER OF SHOOT gene in different radishes. There is a negative correlation between the expression level of the ENHANCER OF SHOOT gene in the radish and the embryo formation rate of the corresponding microspores. When the expression level of the ENHANCER OF SHOOT gene in the measured radish is the lowest, the corresponding microspore embryo formation rate is the highest, and this material is the screened high-embryo-rate radish material. By counting the embryo formation rates of microspores of different radish varieties, the present invention finds that there is a negative correlation between the embryo formation rate of radish microspores and the expression level of the ENHANCER OF SHOOT gene. Based on this, radish varieties with high embryo formation rates can be rapidly screened, effectively saving the manpower and time for screening high-embryo-rate materials.
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Citation Information

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