A strain of candida bombicola and its use

Abstract

The application discloses a strain of Starmerella bombicola and application thereof, and the preservation number of the Starmerella bombicola (Lip-DYH) is CGMCC No. 25797. The Starmerella bombicola provided by the application provides a new kind of yeast and its product can produce a surfactant, and can improve the surface tension of a target solution and improve the antioxidation of the target solution after being mixed with vegetable oil.

Description

Technical Field

[0001] This application belongs to the field of yeast technology, and in particular relates to a strain of bumblebee Candida albicans and its application. Background Technology

[0002] Yeast (saccharomyces) is a commonly used eukaryotic recipient cell in gene cloning experiments, and culturing yeast is as convenient as culturing E. coli. There are also many types of yeast cloning vectors. Yeast also possesses plasmids; these 2μm long plasmids are called 2μm plasmids, approximately 6300 bp in length.

[0003] In related technologies, yeast has the ability to brew wine and ferment. Currently, there are no reports of using yeast and its cultures to improve the performance of cosmetics. Summary of the Invention

[0004] This application provides a bumblebee Candida albicans and its application, which can provide a new yeast and its products that can produce surfactants, improve the surface tension of the target solution contained in the yeast, and enhance its antioxidant properties when mixed with vegetable oils.

[0005] Firstly, this application provides a strain of *Candida albicans* (bumblebee-derived yeast). Starmerella bombicola Lip-DYH, the bumblebee Candida albicans, has the accession number CGMCC No. 25797. The above-mentioned bumblebee Candida albicans ( Starmerella bombicola The deposit date of Lip-DYH is September 26, 2022. The depositary institution is the China Center for Microbial Culture Collection. The deposit address is Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

[0006] According to one embodiment of this application, the gene sequence of *Candida bumblebee* Lip-DYH is shown in SEQ ID NO:1.

[0007] Secondly, embodiments of this application provide a compound microbial agent comprising *Candida bumblebee* Lip-DYH, as described in the first aspect.

[0008] Thirdly, embodiments of this application provide a product containing *Candida bumblebee* Lip-DYH, which is prepared by culturing either *Candida bumblebee* Lip-DYH (as described in the first aspect) or a compound microbial agent (as described in the second aspect).

[0009] According to an embodiment of one aspect of this application, the article comprises at least one of fermentation broth, fermentation broth extract, and surfactant prepared by *Candida bacillus* Lip-DYH.

[0010] According to an embodiment of one aspect of this application, the hydrophilic-lipophilic balance (HLB) of the surfactant is 6-8; the critical micelle concentration of the surfactant is 0.036 g / L.

[0011] According to an embodiment of one aspect of this application, the surfactant comprises mannose erythritol ester.

[0012] Fourthly, embodiments of this application provide a cosmetic composition comprising: oils; and a mixed surfactant prepared by culturing *Candida bumblebee* Lip-DYH, as described in the first aspect.

[0013] According to one embodiment of this application, the oil is a vegetable oil, which includes at least one of rice bran oil, peony seed oil, sacha inchi oil, sunflower seed oil, and prickly pear oil.

[0014] Compared with the prior art, this application has at least the following beneficial effects:

[0015] The bumblebee candidiasis provided in this application ( Starmerella bombicola Lip-DYH can utilize carbon sources such as oils (including vegetable oils), fatty acids, glycerol, glucose, sucrose, and molasses to synthesize surfactants with emulsifying properties. Through fermentation and extraction, fermentation products with good hydrophilic properties and mixed surfactants with good surface activity and emulsifying properties can be obtained. These mixed surfactants can be used in industries such as detergents, daily chemical products, biopesticides, and food to improve oil quality, emulsify, remove contaminants, inhibit bacteria, prevent spoilage, and preserve freshness.

[0016] Furthermore, the antioxidant properties of plant oils can be improved by culturing a mixture of surfactants and plant oils prepared by the first aspect of Candida bacillus Lip-DYH. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments of this application will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This application illustrates the use of *Candida albicans* (a type of yeast) in Example 1. Starmerella bombicola Thin-layer chromatography (TLC) images of the Lip-DYH product;

[0019] Figure 2 This application illustrates the use of *Candida albicans* (a type of yeast) in Example 1. Starmerella bombicola LC-MS analysis spectrum of one of the products of Lip-DYH;

[0020] Figure 3 This application illustrates the use of *Candida albicans* (a type of yeast) in Example 1. Starmerella bombicola NMR spectrum of one of the products of Lip-DYH;

[0021] Figure 4 The embodiments of *Candida albicans* (bumblebee) shown in this application are illustrated. Starmerella bombicola HLB values ​​of surfactants produced by Lip-DYH were measured.

[0022] Figure 5 This application illustrates vegetable oil and *Candida albicans* processed by bumblebee *Candida* (…). Starmerella bombicola A comparison of the hydrophilic diffusion ability of fermented oils after Lip-DYH fermentation;

[0023] Figure 6 This application illustrates vegetable oil and *Candida albicans* processed by bumblebee *Candida* (…). Starmerella bombicola Antioxidant properties of the mixture;

[0024] Figure 7 This application illustrates the use of *Candida albicans* (a type of yeast) in Example 5. Starmerella bombicola A comparison of the emulsification stability of the mixed surfactants generated by Lip-DYH with common emulsifiers. Detailed Implementation

[0025] To make the inventive objectives, technical solutions, and beneficial technical effects of this application clearer, the following detailed description is provided in conjunction with specific embodiments. It should be understood that the embodiments described in this specification are merely illustrative and not intended to limit the scope of this application.

[0026] For simplicity, this paper only explicitly discloses some numerical ranges. However, any lower limit can be combined with any upper limit to form an undefined range; and any lower limit can be combined with other lower limits to form an undefined range, just as any upper limit can be combined with any other upper limit to form an undefined range. Furthermore, although not explicitly stated, every point or individual value between the endpoints of a range is included within that range. Therefore, each point or individual value can serve as its own lower or upper limit and be combined with any other point or individual value, or with other lower or upper limits, to form an undefined range.

[0027] In the description of this article, it should be noted that, unless otherwise stated, "above" and "below" include the number itself, and "several" in "one or several" means two or more.

[0028] The foregoing description of this invention is not intended to describe every disclosed embodiment or implementation. Instead, the following description provides more specific examples of exemplary embodiments. Throughout the application, guidance is provided through a series of embodiments that can be used in various combinations. In each example, the examples are listed only as representative groups and should not be construed as exhaustive.

[0029] Among the related technologies, the microorganisms that have been found to produce biosurfactants are mainly bacteria, while the number of yeasts that produce biosurfactants is relatively small.

[0030] In view of this, this application provides a bumblebee candidiasis yeast that can synthesize a mixed surfactant using various carbon sources.

[0031] Firstly, this application provides a strain of *Candida albicans* (bumblebee-derived yeast). Starmerella bombicola Lip-DYH, the accession number of bumblebee Candida albicans Lip-DYH is CGMCC No. 25797, the accession date is September 26, 2022, the depositary institution is China Culture Collection Center, the depositary address is Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

[0032] According to the embodiments of this application, the colonies of this strain are characterized by being round, smooth, and milky white. Under a microscope, individual cells are oval or round in shape, and budding is visible. *Candida albicans* (bumblebee) Starmerella bombicola Lip-DYH can utilize carbon sources such as oils (including vegetable oils), fatty acids, glycerol, glucose, sucrose, and molasses to synthesize a series of surfactants with emulsifying properties. Through fermentation and extraction, fermentation products with good hydrophilic properties and mixed surfactants with good surface activity and emulsifying properties can be obtained. The extracted fermentation products, including oils and mixed surfactants, can be used in industries such as detergents, daily chemical products, biopesticides, and food for oil improvement, emulsification, detergency, antibacterial and preservative effects, and freshness preservation.

[0033] In some alternative embodiments, the gene sequence of *Candida bumblebee* Lip-DYH is shown in SEQ ID NO:1.

[0034] In some optional embodiments, the method for culturing *Candida albicans* Lip-DYH includes: inoculating *Candida albicans* Lip-DYH into a fermentation medium, fermenting for 72-120 hours under conditions of aeration rate of 0.8-1.2 vvm, stirring speed of 200-600 rpm, and dissolved oxygen ≥10%, to obtain a fermentation broth containing a surfactant. In some embodiments, the amount of *Candida albicans* Lip-DYH inoculated into the fermentation medium is 5%-10% of the fermentation medium. In some embodiments, *Candida albicans* Lip-DYH can be activated *Candida albicans* Lip-DYH. The activation method for *Candida albicans* Lip-DYH includes: culturing *Candida albicans* Lip-DYH seed culture medium at 26-32°C and shaking on a shaker at 200-220 rpm for 20-30 hours.

[0035] The seed culture medium (g / L) comprises, by weight fraction: yeast extract: 2-5 parts, peptone: 3-8 parts, ammonium sulfate: 0.5-2 parts, glycerol: 8-15 parts, and vegetable oil: 10-100 parts. When each of the above components is 1g, add pure metals to 1L to prepare the seed culture medium. Sterilize at pH 6.8-7.0 at 121℃ for 20min.

[0036] The fermentation medium (g / L) comprises, by weight fraction: yeast extract: 2-5 parts, peptone: 1-4 parts, soy protein: 2-5 parts, potassium dihydrogen phosphate: 5-10 parts, dipotassium hydrogen phosphate: 2-5 parts, ammonium sulfate: 0.5-2 parts, glycerol: 8-15 parts, vegetable oil: 300-800 parts, and glucose: 35-40 parts. When each of the above components is 1g, add pure water to a final volume of 1L to prepare the fermentation medium. Sterilize at 121℃ for 30 minutes at pH 6.8-7.0.

[0037] According to the embodiments of this application, the carbon source available in the seed culture medium or fermentation culture medium can be at least one of vegetable oil, waste edible oil, glycerol, molasses, glucose, and sucrose.

[0038] According to the embodiments of this application, Starmerella bombicola Lip-DYH and its related products can be used in the production of detergents and daily chemical products or as ingredients and excipients; they can also be used for antibacterial, antiseptic, and preservation of food and daily chemical raw materials; or as emulsifiers in the pesticide, food, and cosmetic industries; and they can also be used for the treatment of kitchen waste oil such as wastewater containing edible oil, soil, and oily containers.

[0039] Secondly, embodiments of this application provide a compound microbial agent comprising *Candida bumblebee* Lip-DYH, as described in the first aspect.

[0040] Thirdly, embodiments of this application provide a product containing *Candida bumblebee* Lip-DYH, which is prepared by culturing either *Candida bumblebee* Lip-DYH (as described in the first aspect) or a compound microbial agent (as described in the second aspect).

[0041] In some alternative embodiments, the article comprises at least one of fermentation broth, fermentation broth extract, and surfactant prepared by *Candida bacillus* Lip-DYH.

[0042] According to embodiments of this application, the aforementioned surfactant is a metabolite with certain surface activity secreted by *Candida albicans* Lip-DYH under certain conditions during its metabolism. This includes glycolipids, polyglycolipids, lipopeptides, lipoproteins, phospholipids, and neutral lipid derivatives. It possesses the common properties of surfactants, such as solubilization, emulsification, wetting, foaming, dispersion, and surface tension reduction. Compared to other surfactants produced through chemical synthesis or petroleum refining, it also has advantages such as low toxicity, good compatibility with humans and the environment, biodegradability, ecological safety, and high surface activity.

[0043] In some embodiments, the fermentation broth, the fermentation broth extract, and the surfactant prepared by *Candida bumblebee* Lip-DYH each include mannose erythritol esters (MELs). Mannoose erythritol esters are glycolipids with minimum surface tension and interfacial tension reaching 28 mN / m and 2 mN / m, respectively, and also possess antibacterial properties.

[0044] In some alternative embodiments, the surfactant has a hydrophilic-lipophilic balance (HLB) of 6-8 and a critical micelle concentration of 0.036 g / L.

[0045] In some optional embodiments, the surfactant has an antibacterial effect. According to embodiments of this application, by culturing *Candida albicans* (a type of yeast),... Starmerella bombicola The surfactant prepared by Lip-DYH has good antibacterial activity against some Gram-positive bacteria. The minimum inhibitory concentration (MIC) against Streptococcus, Staphylococcus aureus and Bacillus subtilis reached 0.2 mg / ml, 0.5 mg / ml and 1 mg / ml, respectively.

[0046] In some alternative embodiments, the surfactant is prepared by culturing *Candida bumblebee* Lip-DYH in a fermentation medium, optionally with a dissolved oxygen content ≥10%.

[0047] Fourthly, embodiments of this application provide a cosmetic composition comprising: oils; and a mixed surfactant prepared by culturing *Candida bumblebee* Lip-DYH, as described in the first aspect.

[0048] According to the embodiments of this application, when the above-mentioned oils and mixed surfactants are mixed, the surface tension of the oils can be effectively improved to achieve antibacterial effect, and their antioxidant properties can also be improved, giving the cosmetics unique antioxidant properties.

[0049] In some alternative embodiments, the oil is a vegetable oil, including at least one of rice bran oil, peony seed oil, sacha inchi oil, sunflower seed oil, and prickly pear oil. Example

[0050] The following embodiments describe the disclosure of this application in more detail. These embodiments are merely illustrative, as various modifications and variations will be apparent to those skilled in the art within the scope of the disclosure of this application. Unless otherwise stated, all parts, percentages, and ratios reported in the following embodiments are based on weight, and all reagents used in the embodiments are commercially available or synthesized by conventional methods and can be used directly without further processing, and the instruments used in the embodiments are commercially available. Example

[0051] The preserved bacterial strain was activated, and the activated bacterial culture was inoculated into liquid seed culture medium and cultured at 26-32℃ and 200-220 rpm for 24 hours to prepare liquid seed. The prepared liquid seed was transferred into a fermenter containing fermentation medium at an inoculation amount of 5-10%, and fermented at an aeration rate of 0.8-1.2 vvm, a stirring speed of 200-600 rpm, and a dissolved oxygen level of ≥10% for 72-120 hours to obtain a fermentation broth containing surfactant. The fermentation broth was inactivated at 100℃ for 15 minutes, allowed to stand and cool, and the brown precipitate at the bottom was removed using a separatory funnel. 3-5 times the volume of ethanol was added, and the mixture was heated to 50℃ in a water bath and stirred for 30 minutes. The filtrate was filtered and the supernatant was collected. The supernatant was concentrated under reduced pressure at 60℃ to evaporate the solvent and obtain a yellowish-brown oily paste. The obtained product was washed twice with petroleum ether and dried again under reduced pressure to obtain a pale yellow solid. Under the above conditions, a mixed surfactant with an HLB value of 6-8 can be obtained, with a yield of 40-50 g / L. Identified as *Candida bumblebee* by its colony morphology, spore morphology, and the gene shown in SEQ.1. Starmerella bombicola Lip-DYH.

[0052] Seed culture medium (g / L): 2g yeast extract, 5g peptone, 1g ammonium sulfate, 12g glycerol, 50g vegetable oil, added to pure water to a volume of 1L. pH 6.8-7.0, sterilized at 121℃ for 20min;

[0053] Fermentation medium (g / L): 5g yeast extract, 2g peptone, 5g soy protein, 8g potassium dihydrogen phosphate, 1g dipotassium hydrogen phosphate, 12g ammonium sulfate, 12g glycerol, 500g vegetable oil, 40g glucose, add pure water to a volume of 1L, pH 6.8-7.0, sterilize at 121℃ for 30min; the above vegetable oil is prickly ash fruit oil sourced from Shanghai Hongtu Trading Co., Ltd. Example

[0054] The mixed surfactant prepared in Example 1 was analyzed by thin-layer chromatography (TLC), wherein the developing solvent was a mixture of chloroform, methanol, and water, wherein the ratio of chloroform:methanol:water was 65:15:2. After TLC, the mixed surfactant sample was uniformly sprayed with an ethanol solution containing 5% sulfuric acid, the plate was dried, and then baked on a flat plate heater until the spots appeared.

[0055] It was discovered that it possesses a variety of surfactants similar to glycolipids, resulting in... Figure 1 The results are shown. The control substances, namely sucrose fatty acid ester, trehalose lipolipid, rhamnose lipolipid, mannose erythritol ester, and sophorolipid, were purchased from: Aoyuan New Materials Technology Co., Ltd., Shanghai Yusheng Industrial Co., Ltd., Sichuan Sansen Biotechnology Co., Ltd., Shanghai Yusheng Industrial Co., Ltd., and Guangdong Wengjiang Chemical Reagent Co., Ltd., respectively.

[0056] Through separation and purification, Figure 1 As shown, the molecular weight of substance a was determined by LC-MS, and the results are as follows. Figure 2 Substance a was sent to a third party for NMR analysis, and the results were as follows: Figure 3 As shown.

[0057] LC-MS molecular weight determination conditions:

[0058] Using Shimadzu LCMS8045 (EIS source), atomizer flow rate 3L / min, heater flow rate 10L / min, interface temperature 300℃, DL temperature 250℃, heating block temperature 400℃, dry gas flow rate 10L / min, scanning mode: CAN (simultaneous scanning of positive and negative ions m / z: 50-200).

[0059] The results of LC-MS are as follows: Figure 2 The data shows that the sodium ion peak corresponds to a molecular weight of 743-23=720, and the hydrogen anion peak corresponds to 719+1=720, confirming that the corresponding molecular weight is 720. Mannoerythritol esters (MELs) mainly have three structural types, such as... Figure 4 As shown, the molecular weight range is 566-722.

[0060] The NMR 1H spectrum analysis showed the presence of mannose (characteristic shift peaks at 3.57 ppm and 3.60 ppm) and erythritol ester (characteristic shift peak at 3.62 ppm).

[0061] Combining LC-MS and NMR results, *Candida bumblebee* (… Starmerella bombicola The surface-active substances produced by Lip-DYH, CGMCC No. 25797, contain at least one (such as...) Figure 1 The sugar ester (a peak) is mannose erythritol ester, and its structural formula is shown in formula (1). R1 ​​and R2 are independently selected from H- and Ac-, respectively. Ac- represents acetyl group, and n is an integer, n is 6-10.

[0062] Equation (1) Example

[0063] The properties of the hybrid surfactant prepared in Example 1 were tested. Its surface tension, critical micelle concentration (CMC), and HLB value (hydrophilic-lipophilic balance) were measured. The results showed that this hybrid surfactant could reduce the surface tension of water (25°C) from 0.0712 N / m to 0.0387 N / m; its critical micelle concentration was 0.036 g / L. Figure 4 As shown, its HLB value is 6-8, indicating that it has certain hydrophilic properties and can be used as an O / W type emulsifier. Example

[0064] Bumblebee Candida ( Starmerella bombicola The antibacterial effect of Lip-DYH products.

[0065] Bumblebee Candida ( Starmerella bombicola The product of Lip-DYH was prepared into different concentrations of surfactant from 0.2 mg / ml to 500 mg / ml according to the above method, and the minimum inhibitory concentration (MIC) was determined against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus thermophilus, Bacillus subtilis, and Candida albicans, as shown in Table 1.

[0066] Table 1. Minimum inhibitory concentrations of fermentation extracts

[0067]

[0068] In the table, "+" indicates no significant antibacterial effect, and "-" indicates a significant antibacterial effect. Table 1 shows that the mixed surfactants have good antibacterial activity against Staphylococcus aureus, Streptococcus thermophilus, and Bacillus subtilis. They can be used for preservation and antiseptic purposes in food and cosmetics, and can also be used in combination with other biological preservatives. Example

[0069] Improvement of the performance of cosmetic oils: Utilizing bumblebee-derived Candida albicans ( Starmerella bombicola The fermented oil or fermented liquid extract of Lip-DYH, CGMCC No. 25797, is mixed with cosmetic oils at a mass ratio of 1:1000.

[0070] Preserved bumblebee Candida albicans ( Starmerella bombicola The Lip-DYH strain was activated, and the activated culture was inoculated into liquid seed medium and cultured at 26-32℃ and 200-220 rpm for 24 hours to prepare liquid seed. The prepared liquid seed was transferred into a fermenter containing fermentation medium at an inoculation rate of 5-10%. Fermentation was carried out at an aeration rate of 0.8-1.2 vvm, a stirring speed of 200-600 rpm, and dissolved oxygen ≥10% for 72-120 hours. The fermentation broth was inactivated at 100℃ for 15 minutes, allowed to stand and cool, and the upper oil layer was removed using a separatory funnel. 5% diatomaceous earth and 1% anhydrous magnesium sulfate were added to the oil and stirred for 30 minutes. The mixture was then filtered to obtain clear oil.

[0071] This gives the oil better hydrophilic properties compared to the plant oils in the fermentation medium, such as Figure 5 As shown, on a silica gel plate with a certain level of humidity, the diffusion ability of fermented oil is 30% better than that of the original vegetable oil. Therefore, *Candida albicans* (bumblebee yeast) Starmerella bombicola Lip-DYH, CGMCC No. 25797, can be used to improve the properties of plant oils through fermentation, enhance the hydrophilicity of oils, and expand the application of improved fermented oils in skincare products.

[0072] Seed culture medium (g / L): 2g yeast extract, 5g peptone, 1g ammonium sulfate, 12g glycerol, 50g vegetable oil, added to pure water to a volume of 1L. pH 6.8-7.0, sterilized at 121℃ for 20min;

[0073] Fermentation medium (g / L): 5g yeast extract, 2g peptone, 5g soybean protein, 8g potassium dihydrogen phosphate, 1g dipotassium hydrogen phosphate, 12g ammonium sulfate, 12g glycerol, 500ml vegetable oil, 40g glucose, added to pure water to a volume of 1, pH 6.8-7.0, sterilized at 121℃ for 30min; the vegetable oil used in the above seed culture medium and fermentation medium is prickly ash fruit oil from Shanghai Hongtu Trading Co., Ltd. In addition, rice bran oil, peony seed oil, sacha inchi oil and sunflower seed oil also have similar effects.

[0074] Antioxidant properties test:

[0075] DPPH radical scavenging test: 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) radical is a stable, long-lived free radical. Its ethanol solution is deep purple and exhibits strong absorption near 517 nm. In the presence of a DPPH radical scavenger, the light absorption of the DPPH ethanol solution is reduced due to the pairing of unpaired electrons with the radical. The degree of fading of the DPPH ethanol solution is linearly related to the number of electrons it accepts, thus allowing evaluation of the test sample's ability to scavenge free radicals, i.e., the magnitude of its antioxidant activity.

[0076] Table 1. DPPH Experimental Sample Addition Table

[0077]

[0078] The formula for calculating the scavenging rate of DPPH free radicals is as follows:

[0079]

[0080] In the formula:

[0081] T - Absorbance of the sample tube, i.e., the absorbance of the solution after the sample reacts with DPPH;

[0082] T0 - Sample background absorbance;

[0083] The average of three absorbance values ​​of the C-DPPH tube, i.e. the absorbance of the DPPH solution without the addition of sample;

[0084] C0 - Solvent background absorbance.

[0085] The final concentration of the sample in the reaction system was 2%, and the final concentration of the reference vitamin C was 20 ug / ml.

[0086] Bumblebee Candida albicans was cultured using the same vegetable oil in both the seed culture medium and the fermentation medium (Group 1), and with equal amounts of glycerol and glucose. Starmerella bombicola The surfactants obtained by Lip-DYH culture isolation (Group 2) and those obtained by bumblebee Candida albicans ( Starmerella bombicolaThe oils isolated by Lip-DYH culture (plant oils and surfactants, group 3) were subjected to the above antioxidant experiment, and the results are as follows: Figure 6 As shown, this illustrates that the mixture of the two has been processed by bumblebee *Candida* yeast (… Starmerella bombicola Lip-DYH culture, or mixed with *Candida bumblebee* ( Starmerella bombicola The surfactants obtained from Lip-DYH are more effective than those from the original vegetable oils, or even those made solely from bumblebee Candida albicans. Starmerella bombicola Compared with the surfactants obtained from Lip-DYH culture, the antioxidant properties of the plant oil and surfactant group were significantly stronger than those of the other two groups, and the DPPH free radical scavenging rate was significantly improved. This indicates that the combination of cosmetic oils and surfactants obtained from Lip-DYH culture of Starmerella bombicola can comprehensively improve the antioxidant properties of cosmetics.

[0087] Implement column 6

[0088] Using bumblebee Candida albicans ( Starmerella bombicola The fermentation extract of Lip-DYH, CGMCC No. 25797, is used for emulsification in pesticides and cosmetics.

[0089] The preparation method is the same as in Example 1, and the above-mentioned bumblebee Candida albicans ( Starmerella bombicola The emulsification stability of the mixed surfactant prepared by Lip-DYH fermentation was tested. The procedure was as follows: 0.30 g each of Tween 20, polyglycerol-4-laurate, fermentation extract (mixed surfactant), 3.0 g of rice bran oil, and 26.7 g of deionized water were accurately weighed into three test tubes. The mixtures were emulsified at 12000 rpm for 2 min, and the emulsification stability was observed after standing. The results are as follows: Figure 7 As shown.

[0090] according to Figure 3 The results show that the left, middle, and right images represent the initial state of the vegetable oil, the initial state after adding the fermented extract, and the state 10 minutes after emulsification, respectively. From left to right, the images showing the initial state after adding the fermented extract and the state 10 minutes after emulsification represent the groups with Tween 20, polyglycerol-4-laurate, and the group with the fermented extract, respectively. Both the Tween 20 and polyglycerol-4-laurate groups showed significant stratification 10 minutes after emulsification, while the fermented extract (mixed surfactant) group exhibited relatively better emulsification with less noticeable stratification. Therefore, its emulsification stability is superior to that of equal amounts of commonly used emulsifiers such as Tween 20 and polyglycerol-4-laurate.

[0091] It should also be noted that the exemplary embodiments mentioned in this application describe methods or systems based on a series of steps or apparatus. However, this application is not limited to the order of the above steps; that is, the steps can be performed in the order mentioned in the embodiments, or in a different order, or several steps can be performed simultaneously.

[0092] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this application, and these modifications or substitutions should all be covered within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A bumblebee-borne Candida albicans ( Starmerella bombicola Lip-DYH, characterized in that, The bumblebee Candida albicans Lip-DYH has the accession number CGMCC No. 25797.

2. The bumblebee Candida albicans Lip-DYH according to claim 1, characterized in that, The gene sequence of the bumblebee Candida Lip-DYH is shown in SEQ ID NO:

1.

3. A compound microbial agent, characterized in that, It contains the bumblebee Candida Lip-DYH as described in claim 1 or 2.

4. A hybrid surfactant, characterized in that, It was prepared by culturing the bumblebee Candida albicans Lip-DYH as described in claim 1 or 2.

5. The mixed surfactant according to claim 4, characterized in that, The hydrophilic-lipophilic balance (HLB) of the mixed surfactant is 6-8; the critical micelle concentration of the mixed surfactant is 0.036 g / L.

6. The mixed surfactant according to claim 5, characterized in that, The mixed surfactant contains mannose erythritol ester.

7. A cosmetic composition, characterized in that, The cosmetic composition comprises: oils and a mixed surfactant as described in any one of claims 4 to 6.

8. The cosmetic composition according to claim 7, characterized in that, The oil is a vegetable oil, which includes at least one of rice bran oil, peony seed oil, sacha inchi oil, sunflower seed oil, and prickly pear oil.

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