A hair dye for preventing hair breakage, hypoallergenic and repairing

Abstract

The present invention relates to the technical field of hair dyes, and in particular to a hair dye for preventing hair breakage, hypoallergenicity and repairing. The hair dye of the present invention adopts propylene glycol, polyquaternium salt, camellia oil, caprylic / capric triglyceride, trehalose, glycerin, hydrolyzed wheat protein, ginseng extract, seabuckthorn leaf extract, andrographis paniculata extract, mangosteen bark extract and other active ingredients with moisturizing, anti-inflammatory and hair repairing properties to prepare the hair dye of the present invention, and the hair dye has excellent effects of soothing the skin, moisturizing the skin, and repairing the hair quality and making the hair grow continuously.

Description

Technical Field

[0001] The invention relates to the technical field of hair dyes, and in particular to a hair dye for preventing hair breakage, reducing allergies and repairing hair. Background Art

[0002] Hair dye is a hair cosmetic that changes the color of hair. There are three different types of hair dyes: temporary, semi-permanent, and permanent. Hair dye has a variety of hair colors to choose from. Hair dyeing is not only about dyeing black hair into various colors such as gold, yellow, flax, reddish brown, coffee, brown, etc. that suit your skin color and personality to show your charm, but also about turning the gray hair of the elderly black.

[0003] Currently, hair dyes often use irritating raw materials such as hydrogen peroxide, which makes them highly irritating and poses a challenge to skin safety. Therefore, providing a hair dye that soothes the skin, has low allergy, prevents hair breakage, and repairs hair is a technical problem that needs to be solved. Summary of the invention

[0004] The present invention aims at the above-mentioned technical problems and proposes a technical solution of the present invention, which includes an anti-hair breakage, hypoallergenic and repairing hair dye and a preparation method thereof.

[0005] The hair dye of the present invention adopts propylene glycol, polyquaternium salt, camellia oil, caprylic / capric triglyceride, trehalose, glycerin, hydrolyzed wheat protein, ginseng extract, seabuckthorn leaf extract, andrographis paniculata extract, mangosteen bark extract and other active ingredients with moisturizing, anti-inflammatory and hair repairing effects to prepare the hair dye product of the present invention.

[0006] The hair dye for preventing hair breakage, reducing allergy and repairing hair of the present invention comprises the following components:

[0007] The first component includes propylene glycol, fatty alcohol polyoxyethylene ether, hydrogen peroxide, sodium stannate and water.

[0008] The second component: including p-phenylenediamine, sodium sulfite, polyquaternium, camellia oil, caprylic / capric triglyceride, trehalose, glycerin, hydrolyzed wheat protein, carbomer, triethanolamine, p-aminophenol, coconut acid diethanolamide, alkyl glucoside, ginseng extract, sea buckthorn leaf extract, andrographis paniculata extract, sodium citrate, and water.

[0009] Preferably, the mass ratio of the first component to the second component is 1:0.7-1.2.

[0010] Preferably, in the first component, the weight proportions of the components are: 0.5-2.8 parts of propylene glycol, 2-4 parts of fatty alcohol polyoxyethylene ether, 3-7 parts of hydrogen peroxide, 0.02-0.1 parts of sodium stannate, and 35-55 parts of water.

[0011] Preferably, in the second component, the weight parts of each ingredient are: 0.5-1.5 parts of p-phenylenediamine, 0.7-2.2 parts of sodium sulfite, 0.2-1 parts of polyquaternium salt, 1-3 parts of camellia oil, 1-3 parts of caprylic / capric triglyceride, 2-5 parts of trehalose, 2-4 parts of glycerol, 0.5-1.8 parts of hydrolyzed wheat protein, 1-2 parts of carbomer, 0.5-1.8 parts of triethanolamine, 0.05-0.37 parts of p-aminophenol, 1.5-2.5 parts of coconut acid diethanolamide, 1-2.5 parts of alkyl glycosides, 0.05-1 parts of ginseng extract, 0.05-1 parts of sea buckthorn leaf extract, 0.05-1 parts of andrographis paniculata extract, 0.05-0.8 parts of mangosteen bark extract, 0.2-1 parts of sodium citrate, and 40-60 parts of water.

[0012] Preferably, the mass ratio of the first component to the second component is 1:0.8-1.1.

[0013] Preferably, the mass ratio of the Andrographis paniculata extract to the Mangosteenia mangosteen bark extract is 1.2-2.5:1.

[0014] Preferably, the preparation method of Andrographis paniculata extract comprises the following steps: crushing Andrographis paniculata, adding water and cellulase to obtain a mixed solution, performing a first enzymolysis and inactivating the enzymes; adding neutral protease and trypsin to obtain a mixture, performing a second enzymolysis and inactivating the enzymes to obtain an enzymolysis product; adding ethanol, heating and stirring, coarse filtering, ultrafiltration, and freeze-drying the ultrafiltrate to obtain the Andrographis paniculata extract.

[0015] Preferably, the preparation method of Andrographis paniculata extract comprises the following steps: crushing Andrographis paniculata to obtain a crushed material, adding 10-30 times the mass of water and 0.05-0.2 times the mass of cellulase in the crushed material to obtain a mixed solution, performing enzymolysis at 32-40°C for 5-12 hours, performing the first enzymolysis, inactivating the enzymes, and obtaining an enzyme-inactivated material; adding 0.5-3% of the mass of neutral protease and 0.2-4% of trypsin in the mass of the enzyme-inactivated material to obtain a mixture, performing enzymolysis at 30-38°C for 4-9 hours, inactivating the enzymes, and obtaining an enzymolysis product; adding 0.25-0.6 times the mass of ethanol in the mass of the enzymolysis product, heating to 35-55°C and stirring for 4-10 hours, coarse filtering, ultrafiltration with a 2KDa molecular weight ultrafiltration membrane, and freeze-drying the ultrafiltrate to obtain the Andrographis paniculata extract.

[0016] Preferably, the preparation method of the Mangosteen bark extract comprises the following steps: crushing the Mangosteen bark, adding ethanol aqueous solution, heating and stirring, coarse filtering, ultrafiltration, and freeze-drying the ultrafiltrate to obtain the Mangosteen bark extract.

[0017] Preferably, the preparation method of the bark extract of Mangosteen tree comprises the following steps: crushing the bark of Mangosteen tree, adding 30-45% ethanol aqueous solution with a mass concentration of 12-25 times the mass of the crushed material, heating to 40-58°C and stirring for 6-12 hours, coarse filtering, ultrafiltration with a 1.5KDa molecular weight ultrafiltration membrane, and freeze-drying the ultrafiltrate to obtain the bark extract of Mangosteen tree.

[0018] The present invention also provides a method for preparing the anti-hair breakage, hypoallergenic and repairing hair dye, comprising the following preparation steps: preparing a first component, preparing a second component, and storing the first and second components separately.

[0019] Preferably, preparing the first component comprises adding water into a container, adding propylene glycol, fatty alcohol polyoxyethylene ether, and sodium stannate and stirring, and then adding hydrogen peroxide and continuing to stir to obtain the first component.

[0020] Preferably, the preparation of the second component comprises adding water into a container, adding glycerol, trehalose, carbomer, triethanolamine, coconut oil diethanolamide, alkyl glucoside, polyquaternium salt, p-phenylenediamine, sodium sulfite, p-aminophenol, sodium citrate, stirring; then adding the remaining raw materials, continuing stirring, sterilizing, and obtaining the second component.

[0021] Beneficial effects of the present invention:

[0022] The hair dye of the present invention adopts active ingredients with moisturizing, anti-inflammatory and hair repairing effects, such as propylene glycol, polyquaternium salt, camellia oil, caprylic / capric triglyceride, trehalose, glycerin, hydrolyzed wheat protein, ginseng extract, seabuckthorn leaf extract, andrographis paniculata extract, and mangosteen bark extract, to prepare the hair dye of the present invention. The hair dye has excellent effects of soothing the skin, moisturizing the skin and repairing the hair to ensure hair growth.

[0023] The Andrographis paniculata extract and the Mangosteenia mangosteen bark extract prepared by the present invention both have good anti-inflammatory and skin-soothing effects, and impart low irritation to cosmetic products.

[0024] When the diluted Andrographis paniculata extract prepared by the present invention was subjected to HaCaT cell experiment, the concentrations of TNF-α and IL-1β in the cell supernatant obtained by the test were as low as 576.28 ng / L and 70.43 ng / L. Compared with the model group, the proportion of reducing the concentration of inflammatory factors TNF-α and IL-1β reached 17.36% and 26.50%.

[0025] When the dilution of the extract of the bark of Mangosteen prepared by the present invention is subjected to HaCaT cell experiment, the concentrations of TNF-α and IL-1β in the cell supernatant obtained by the test are as low as 578.73 ng / L and 70.92 ng / L. Compared with the model group, the proportion of reducing the concentration of inflammatory factors TNF-α and IL-1β is 17.01% and 25.99%.

[0026] The present invention adopts neutral protease combined with trypsin to synergistically enhance the anti-inflammatory effect of Andrographis paniculata extract; and when ethanol is added to the enzymatic hydrolysate to adjust the ethanol dosage to 0.25-0.6 times of the enzymatic hydrolysate, the anti-inflammatory effect of Andrographis paniculata extract can also be enhanced. The selection of the process of the present invention gives Andrographis paniculata extract excellent anti-inflammatory skin, and gives the skin a low-sensitivity and low-irritation effect.

[0027] The invention adopts ethanol aqueous solution extraction and combines interception to select extracts with specific molecular weight, thereby giving the mangosteen bark extract a better anti-inflammatory effect.

[0028] The anti-inflammatory effect of the Andrographis paniculata extract and the Mangosteenia oleifera bark extract prepared by the present invention is enhanced after being combined. The mass ratio of the two is in the range of 1.2-2.5:1, and the efficiency of reducing the concentration of inflammatory factors is higher. The best effect can be achieved when the mass ratio is 2:1.

[0029] The hair dye of the present invention has a good hair dyeing effect and has the effect of moisturizing hair, giving the hair a non-dry and rough effect. DETAILED DESCRIPTION

[0030] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0031] 1. Preparation of Andrographis paniculata Extract

[0032] Andrographis extract 1:

[0033] The preparation method comprises the following steps: crushing andrographis paniculata to obtain a crushed material with a particle size of less than 0.1 mm, adding 20 times the mass of water and 0.15 times the mass of cellulase (100,000 U / g) of the crushed material to obtain a mixed solution, stirring at 37°C and 75 rpm for 10 hours for enzymolysis, performing the first enzymolysis, inactivating the enzyme, and obtaining an enzyme-inactivated material; adding 2.5% of the mass of the enzyme-inactivated material, neutral protease (50,000 U / g) and 3% of trypsin (50,000 U / g) to obtain a mixture, stirring at 35°C and 75 rpm for 7.5 hours for the second enzymolysis, inactivating the enzyme, and obtaining an enzymolysis product; adding 0.5 times the mass of the enzymolysis product, anhydrous ethanol, heating to 52°C, stirring at 80 rpm for 8 hours, coarse filtering with a 2-micron filter membrane, ultrafiltration with a 2KDa molecular weight ultrafiltration membrane, freeze-drying the ultrafiltrate at -40°C to a water content of about 4.78wt%, and obtaining an andrographis paniculata extract.

[0034] Andrographis Extract 2:

[0035] The preparation method comprises the following steps: crushing andrographis paniculata to obtain a crushed material with a particle size of less than 0.1 mm, adding 25 times the mass of water and 0.1 times the amount of cellulase (150,000 U / g) of the crushed material to obtain a mixed solution, stirring at 38°C and 80 rpm for enzymolysis for 12 hours, performing a first enzymolysis, inactivating the enzyme, and obtaining an enzyme-inactivated material; adding 2.2% of the mass of the enzyme-inactivated material, neutral protease (60,000 U / g) and 3.5% of trypsin (30,000 U / g) to obtain a mixture, stirring at 38°C and 80 rpm for enzymolysis for 8 hours, inactivating the enzyme, and obtaining an enzymolysis product; adding 0.3 times the mass of the enzymolysis product, anhydrous ethanol, heating to 55°C, stirring at 70 rpm for 7.5 hours, coarse filtering with a 2-micron filter membrane, ultrafiltration with a 2KDa molecular weight ultrafiltration membrane, freeze-drying the ultrafiltrate at -40°C to a water content of about 4.59wt%, and obtaining an andrographis paniculata extract.

[0036] Andrographis extract 3:

[0037] The preparation method comprises the following steps: crushing andrographis paniculata to obtain a crushed material with a particle size of less than 0.1 mm, adding 20 times the mass of water and 0.15 times the mass of cellulase (100,000 U / g) of the crushed material to obtain a mixed solution, stirring at 37°C and 75 rpm for 10 hours for enzymolysis, inactivating the enzyme, and obtaining an enzyme-inactivated material; adding 5.5% of the mass of the enzyme-inactivated material, a neutral protease (50,000 U / g), to obtain a mixture, stirring at 35°C and 75 rpm for 7.5 hours for enzymolysis, inactivating the enzyme, and obtaining an enzyme hydrolysis product; adding 0.5 times the mass of the enzyme hydrolysis product, anhydrous ethanol, heating to 52°C, stirring at 80 rpm for 8 hours, coarse filtering with a 2-micron filter membrane, ultrafiltration with a 2KDa molecular weight ultrafiltration membrane, freeze-drying the ultrafiltrate at -40°C to a water content of about 4.78wt%, and obtaining an andrographis paniculata extract.

[0038] Andrographis extract 4:

[0039] The preparation method comprises the following steps: crushing andrographis paniculata to obtain a crushed material with a particle size of less than 0.1 mm, adding 20 times the mass of water and 0.15 times the mass of cellulase (100,000 U / g) of the crushed material to obtain a mixed solution, stirring at 37°C and 75 rpm for 10 hours for enzymolysis, inactivating the enzyme, and obtaining an enzyme-inactivated material; adding 5.5% of the mass of trypsin (50,000 U / g) of the enzyme-inactivated material to obtain a mixture, stirring at 35°C and 75 rpm for 7.5 hours for enzymolysis, inactivating the enzyme, and obtaining an enzyme hydrolysis product; adding 0.5 times the mass of anhydrous ethanol of the enzyme hydrolysis product, heating to 52°C, stirring at 80 rpm for 8 hours, coarse filtering with a 2-micron filter membrane, ultrafiltration with a 2KDa molecular weight ultrafiltration membrane, freeze-drying the ultrafiltrate at -40°C to a water content of about 4.78wt%, and obtaining an andrographis paniculata extract.

[0040] Andrographis extract 5:

[0041] The preparation method comprises the following steps: crushing andrographis paniculata to obtain a crushed material with a particle size of less than 0.1 mm, adding 20 times the mass of water and 0.15 times the mass of cellulase (100,000 U / g) of the crushed material to obtain a mixed solution, stirring at 37°C and 75 rpm for 10 hours for enzymolysis, performing the first enzymolysis, inactivating the enzyme, and obtaining an enzymolysis product; adding 0.5 times the mass of the enzymolysis product of anhydrous ethanol, heating to 52°C, stirring at 80 rpm for 8 hours, coarse filtering with a 2-micron filter membrane, ultrafiltration with a 2KDa molecular weight ultrafiltration membrane, freeze-drying the ultrafiltrate at -40°C to a water content of about 4.78wt%, and obtaining an andrographis paniculata extract.

[0042] Andrographis paniculata extract6:

[0043] The preparation method comprises the following steps: crushing andrographis paniculata to obtain a crushed material with a particle size of less than 0.1 mm, adding 20 times the mass of water and 0.15 times the mass of cellulase (100,000 U / g) of the crushed material to obtain a mixed solution, stirring at 37°C and 75 rpm for enzymolysis for 10 hours, performing the first enzymolysis, inactivating the enzyme, and obtaining an enzyme-inactivated material; adding 2.5% of the mass of the enzyme-inactivated material, neutral protease (50,000 U / g) and 3% of trypsin (50,000 U / g) to obtain a mixture, stirring at 35°C and 75 rpm for enzymolysis for 7.5 hours, inactivating the enzyme, and obtaining an enzymolysis product; adding 0.5 times the mass of the enzymolysis product, heating to 52°C, stirring at 80 rpm for 8 hours, coarse filtering with a 2-micron filter membrane, ultrafiltration with a 2KDa molecular weight ultrafiltration membrane, freeze-drying the ultrafiltrate at -40°C to a water content of about 4.78wt%, and obtaining an andrographis paniculata extract.

[0044] Andrographis extract 7:

[0045] The preparation method comprises the following steps: crushing andrographis paniculata to obtain a crushed material with a particle size of less than 0.1 mm, adding 20 times the mass of water and 0.15 times the mass of cellulase (100,000 U / g) of the crushed material to obtain a mixed solution, stirring at 37°C and 75 rpm for 10 hours for enzymolysis, performing the first enzymolysis, inactivating the enzyme, and obtaining an enzyme-inactivated material; adding 2.5% of the mass of the enzyme-inactivated material, neutral protease (50,000 U / g) and 3% of trypsin (50,000 U / g) to obtain a mixture, stirring at 35°C and 75 rpm for 7.5 hours for the second enzymolysis, inactivating the enzyme, and obtaining an enzymolysis product; adding 0.1 times the mass of the enzymolysis product, anhydrous ethanol, heating to 52°C, stirring at 80 rpm for 8 hours, coarse filtering with a 2-micron filter membrane, ultrafiltration with a 2KDa molecular weight ultrafiltration membrane, freeze-drying the ultrafiltrate at -40°C to a water content of about 4.78wt%, and obtaining an andrographis paniculata extract.

[0046] Andrographis extract 8:

[0047] The preparation method comprises the following steps: crushing andrographis paniculata to obtain a crushed material with a particle size of less than 0.1 mm, adding 20 times the mass of water and 0.15 times the mass of cellulase (100,000 U / g) of the crushed material to obtain a mixed solution, stirring at 37°C and 75 rpm for 10 hours for enzymolysis, performing the first enzymolysis, inactivating the enzyme, and obtaining an enzyme-inactivated material; adding 2.5% of the mass of the enzyme-inactivated material, neutral protease (50,000 U / g) and 3% of trypsin (50,000 U / g) to obtain a mixture, stirring at 35°C and 75 rpm for 7.5 hours for the second enzymolysis, inactivating the enzyme, and obtaining an enzymolysis product; adding 1 times the mass of the enzymolysis product, anhydrous ethanol, heating to 52°C, stirring at 80 rpm for 8 hours, coarse filtering with a 2-micron filter membrane, ultrafiltration with a 2KDa molecular weight ultrafiltration membrane, freeze-drying the ultrafiltrate at -40°C to a water content of about 4.78wt%, and obtaining an andrographis paniculata extract.

[0048] Andrographis extract 9:

[0049] The preparation method comprises the following steps: crushing andrographis paniculata to obtain a crushed material with a particle size of less than 0.1 mm, adding 20 times the mass of water and 0.15 times the mass of cellulase (100,000 U / g) of the crushed material to obtain a mixed solution, stirring at 37°C and 75 rpm for 10 hours for enzymolysis, performing the first enzymolysis, inactivating the enzyme, and obtaining an enzyme-inactivated material; adding 2.5% of the mass of the enzyme-inactivated material, neutral protease (50,000 U / g) and 3% of trypsin (50,000 U / g) to obtain a mixture, stirring at 35°C and 75 rpm for 7.5 hours for the second enzymolysis, inactivating the enzyme, and obtaining an enzymolysis product; adding 1.5 times the mass of the enzymolysis product, anhydrous ethanol, heating to 52°C, stirring at 80 rpm for 8 hours, coarse filtering with a 2-micron filter membrane, ultrafiltration with a 2KDa molecular weight ultrafiltration membrane, freeze-drying the ultrafiltrate at -40°C to a water content of about 4.78wt%, and obtaining an andrographis paniculata extract.

[0050] 2. Preparation of extract from bark of Mangosteen

[0051] Mangosteen bark extract1:

[0052] The preparation method comprises the following steps: crushing the bark of the koreana mangostana to less than 0.1 mm, adding an ethanol aqueous solution with a mass concentration of 40% which is 20 times the mass of the crushed material, heating to 50° C., stirring at 75 rpm for 8 hours, coarse filtering with a 2-micron filter membrane, ultrafiltration of the coarse filtrate through an ultrafiltration membrane with a molecular weight cutoff of 1.5 KDa, and freeze-drying the ultrafiltrate at -40° C. to a water content of about 4.25 wt%, thereby obtaining the koreana mangostana bark extract.

[0053] Mangosteen bark extract2:

[0054] The preparation method comprises the following steps: crushing the bark of the koreana tree to less than 0.1 mm, adding a 30% ethanol aqueous solution with a mass concentration of 30 times the mass of the crushed material, heating to 47° C., stirring at 75 rpm for 9.5 hours, coarse filtering with a 2-micron filter membrane, ultrafiltration of the coarse filtrate through an ultrafiltration membrane with a molecular weight cutoff of 1.5 KDa, and freeze-drying the ultrafiltrate at -40° C. to a water content of about 4.18 wt%, to obtain the koreana tree bark extract.

[0055] 3. Anti-inflammatory effect test

[0056] 1. HaCaT cells were cultured in DMEM at 37°C and 5% CO2 for 48 hours, and then the culture medium was replaced until the cell confluence reached about 90%. Then, the cells were configured into the following groups according to the specific experimental content:

[0057] Blank group: continue to culture using the above method.

[0058] Model group: LPS was added to make its concentration in the culture medium reach 1 μg / mL, cultured for 24 h, and then replaced with new DMEM cell culture medium and cultured for another 24 h.

[0059] Positive drug group: LPS was added to make its concentration in the culture medium 1 μg / mL, cultured for 24 h, and then replaced with DMEM cell culture medium supplemented with 10 μg / mL dexamethasone and cultured for another 24 h.

[0060] Extract group: a group of the above extracts:

[0061] Andrographis extract 1 group: LPS was added to the culture medium at a concentration of 1 μg / mL, and the cells were cultured for 24 h. The culture medium was then replaced with DMEM cell culture medium supplemented with 200 μg / mL of Andrographis extract 1 and cultured for another 24 h.

[0062] Andrographis extract 2 group: LPS was added to the culture medium at a concentration of 1 μg / mL, and the cells were cultured for 24 h. The culture medium was then replaced with DMEM cell culture medium supplemented with 200 μg / mL of Andrographis extract 2 and cultured for another 24 h.

[0063] The other Andrographis paniculata extract and Mangosteenia mangosteen bark extract groups were prepared and cultured according to the method of Andrographis paniculata extract group 1-2 in the experimental group.

[0064] The cell supernatant after the above culture was taken, and the concentration of TNF-α and IL-1β in the supernatant was detected using an ELISA kit. The results are shown in Table 1.

[0065] Table 1: Concentrations of TNF-α and IL-1β in the supernatant of each group

[0066]

[0067]

[0068] According to the contents of Table 1, the data of each group were converted to calculate the reduction ratio of the concentration of TNF-α and IL-1β in each group compared with the model group. The results are shown in Table 2.

[0069] Table 2: The reduction ratio of TNF-α and IL-1β concentrations in each group compared with the model group

[0070]

[0071]

[0072] According to the test results in Tables 1 and 2, it can be concluded that the Andrographis paniculata extract and the Mangosteenia mangosteen bark extract prepared by the present invention both have good anti-inflammatory and skin-soothing effects, and impart low irritation to the cosmetic products.

[0073] When the diluted Andrographis paniculata extract prepared by the present invention was subjected to HaCaT cell experiment, the concentrations of TNF-α and IL-1β in the cell supernatant obtained by the test were as low as 576.28 ng / L and 70.43 ng / L. Compared with the model group, the proportion of reducing the concentration of inflammatory factors TNF-α and IL-1β reached 17.36% and 26.50%.

[0074] When the dilution of the extract of the bark of Mangosteen prepared by the present invention was subjected to HaCaT cell experiment, the concentrations of TNF-α and IL-1β in the cell supernatant obtained by the test were as low as 578.73 ng / L and 70.92 ng / L. Compared with the model group, the proportion of reducing the concentration of inflammatory factors TNF-α and IL-1β reached 17.01% and 25.99%.

[0075] The test results of Andrographis paniculata extracts 1-9 in Table 1-2 show that the present invention uses neutral protease combined with trypsin to synergistically enhance the anti-inflammatory effect of Andrographis paniculata extract; and when ethanol is added to the enzymatic hydrolysate to adjust the ethanol dosage to 0.25-0.6 times that of the enzymatic hydrolysate, the anti-inflammatory effect of Andrographis paniculata extract can also be enhanced. The selection of the process of the present invention gives Andrographis paniculata extract excellent anti-inflammatory skin, and gives the skin a low-sensitivity and low-irritation effect.

[0076] The invention adopts ethanol aqueous solution extraction and combines interception to select extracts with specific molecular weight, thereby giving the mangosteen bark extract a better anti-inflammatory effect.

[0077] 2. Combine Andrographis paniculata extract 1 and Mangosteen bark extract 1 to study the combination effect of the two. According to the relevant test in the above test 1, set the group: add LPS so that its concentration in the culture medium is 1 μg / mL, culture for 24 hours, and then replace it with DMEM cell culture medium added with 200 μg / mL of extract (the extract is prepared by combining Andrographis paniculata extract 1 and Mangosteen bark extract 1 in different dosage ratios) and continue to culture for 24 hours. Then use the above method to test the concentrations of TNF-α and IL-1β, the anti-inflammatory effect indicators after the combination of the two, and the results are shown in Table 3.

[0078] Table 3: Concentrations of TNF-α and IL-1β in different combinations

[0079]

[0080]

[0081] According to the test results in Table 3, the anti-inflammatory effect of the Andrographis paniculata extract and the Mangosteenia oleifera bark extract prepared by the present invention is enhanced after being combined, and the mass ratio of the two is in the range of 1.2-2.5:1, and the efficiency of reducing the concentration of inflammatory factors is higher, and the best effect can be achieved when the mass ratio is 2:1.

[0082] 4. Preparation and testing of hair dyes

[0083] The composition of the hair dye is shown in Table 4. Camellia oil: Wuhan Lvjing Fenghua Biotechnology Co., Ltd. Trehalose: Zhongyan (Shandong) New Materials Co., Ltd. Hydrolyzed wheat protein: Shenzhen Xingkaiyue Biotechnology Co., Ltd. Ginseng extract: Kunshan Sheng'an Biotechnology Co., Ltd. Seabuckthorn leaf extract: Shaanxi Junhe Biotechnology Co., Ltd.

[0084] Table 4: Composition of hair dye

[0085]

[0086]

[0087] The method for preparing the hair dye comprises the following steps: preparing a first component, preparing a second component, and storing the first and second components separately;

[0088] The first component is prepared by adding water into a container, adding propylene glycol (if present), fatty alcohol polyoxyethylene ether, and sodium stannate, stirring at 80 rpm for 0.2 hours, and then adding hydrogen peroxide and continuing stirring for 0.2 hours to obtain the first component;

[0089] The preparation of the second component comprises adding water into a container, adding glycerol (if present), trehalose (if present), carbomer, triethanolamine, coconut oil diethanolamide, alkyl glycoside, polyquaternium salt (if present), p-phenylenediamine, sodium sulfite, p-aminophenol, sodium citrate, stirring at 75 rpm for 0.5 hour; then adding the remaining raw materials, continuing stirring for 0.3 hour, sterilizing, and obtaining the second component.

[0090] The hair dye was subjected to the following performance test: the first component and the second component were used to dye the isolated hair bundles according to the mass ratio, and then left to stand for 5 minutes, and then treated at 45°C for 20 minutes, cooled naturally, rinsed with water, and blow-dried to complete the experimental steps. Then 20 volunteers were selected to evaluate the coloring effect and wettability of the isolated hair bundles treated with each hair dye, with the highest score being 10 points and the lowest score being 1 point, wherein the higher the evaluation score, the better the coloring effect and the better the hair wetting effect after dyeing. The results of the hair dye effect test are shown in Table 5.

[0091] Table 5: Hair dye effect test

[0092] test Coloring effect evaluation Wettability evaluation Hair Dye 1 9.1 8.7 Hair Dye 2 9.2 8.5 Hair Dye 3 8.9 7.1

[0093] It can be seen from the test results in Table 5 that the hair dye of the present invention has a good hair dyeing effect and has the effect of moisturizing hair, giving the hair a non-dry and rough effect.

[0094] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A hair dye for preventing hair breakage, reducing allergy and repairing, characterized in that: The components include: The first component: includes propylene glycol, fatty alcohol polyoxyethylene ether, hydrogen peroxide, sodium stannate and water; The second component: including p-phenylenediamine, sodium sulfite, polyquaternium salt, camellia oil, caprylic / capric triglyceride, trehalose, glycerin, hydrolyzed wheat protein, carbomer, triethanolamine, p-aminophenol, coconut acid diethanolamide, alkyl glucoside, ginseng extract, sea buckthorn leaf extract, andrographis paniculata extract, mangosteen bark extract, sodium citrate and water; The mass ratio of the first component to the second component is 1:0.7-1.3; In the first component, the weight parts of each component are: 0.5-2.8 parts of propylene glycol, 2-4 parts of fatty alcohol polyoxyethylene ether, 3-7 parts of hydrogen peroxide, 0.02-0.1 parts of sodium stannate and 35-55 parts of water; In the second component, the weight parts of each component are: 0.5-1.5 parts of p-phenylenediamine, 0.7-2.2 parts of sodium sulfite, 0.2-1 parts of polyquaternium salt, 1-3 parts of camellia oil, 1-3 parts of caprylic / capric triglyceride, 2-5 parts of trehalose, 2-4 parts of glycerol, 0.5-1.8 parts of hydrolyzed wheat protein, 1-2 parts of carbomer, 0.5-1.8 parts of triethanolamine, 0.05-0.37 parts of p-aminophenol, 1.5-2.5 parts of coconut acid diethanolamide, 1-2.5 parts of alkyl glucoside, 0.05-1 parts of ginseng extract, 0.05-1 parts of sea buckthorn leaf extract, 0.05-1 parts of andrographis paniculata extract, 0.05-0.8 parts of mangosteen bark extract, 0.2-1 parts of sodium citrate and 40-60 parts of water; The preparation method of the Andrographis paniculata extract comprises the following steps: crushing the Andrographis paniculata to obtain a crushed material, adding water 10-30 times the mass of the crushed material and 0.05-0.2 times the cellulase to obtain a mixed solution, performing enzymolysis at 32-40°C for 5-12 hours, performing a first enzymolysis, inactivating the enzyme, and obtaining an enzyme-inactivated material; adding 0.5-3% of the mass of the enzyme-inactivated material with a neutral protease and 0.2-4% of the trypsin to obtain a mixture, performing enzymolysis at 30-38°C for 4-9 hours, inactivating the enzyme, and obtaining an enzymolysis product; adding ethanol 0.25-0.6 times the mass of the enzymolysis product, heating to 35-55°C and stirring for 4-10 hours, coarse filtering, performing ultrafiltration with a 2KDa molecular weight ultrafiltration membrane, and freeze-drying the ultrafiltrate to obtain the Andrographis paniculata extract; The preparation method of the bark extract of the koreana mangostana comprises the following steps: crushing the bark of the koreana mangostana, adding an ethanol aqueous solution with a mass concentration of 30-45% and a mass of 12-25 times the mass of the crushed material, heating to 40-58° C. and stirring for 6-12 hours, coarse filtering, ultrafiltration with a 1.5KDa molecular weight ultrafiltration membrane, and freeze-drying the ultrafiltrate to obtain the bark extract of the koreana mangostana.

2. The hair dye according to claim 1, characterized in that The mass ratio of the first component to the second component is 1:0.8-1.

25.

3. The hair dye according to claim 1, characterized in that The mass ratio of the Andrographis paniculata extract to the Mangosteenia truncatula bark extract is 1.2-2.5:

1.

4. A method for preparing the hair dye according to any one of claims 1 to 3, characterized in that: The method comprises the following preparation steps: preparing a first component, preparing a second component, and storing the first and second components separately.

5. The preparation method according to claim 4, characterized in that: The preparation of the first component includes adding water into a container, adding propylene glycol, fatty alcohol polyoxyethylene ether, and sodium stannate, stirring, and then adding hydrogen peroxide and continuing to stir to obtain the first component.

6. The preparation method according to claim 4, characterized in that: The preparation of the second component comprises adding water into a container, adding glycerol, trehalose, carbomer, triethanolamine, coconut oil diethanolamide, alkyl glucoside, polyquaternium salt, p-phenylenediamine, sodium sulfite, p-aminophenol, sodium citrate, stirring; then adding the remaining raw materials, continuing stirring, sterilizing, and obtaining the second component.

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